Mohammed Abozeid, Ashraf Ahmed, M. Alwany, M. El-Sherbiny, Belal Saleh
Age and Growth of the fish Epinephelus fasciatus (n=443) from Northern Red Sea were investigated, during the period from June 2006 to May 2007. The t-test analysis for the length-weight relationships showed a significant difference between b values, where male are positive allometric while female and sexes combined are isometric. The "Kc" and (Kn) factor was studied monthly and according to size classes, where females usually outnumbered males. The total average of Kc for sexes combined was 1.428 (SE.± 0.4134), this value is indication of a good condition. The value for both Kc and Kn increased in line with increased fish size until the size 31.4, and then decreased in value. On the other hand, both condition factors showed fluctuations among months, where the lowest value was recorded during August (within spawning season). The back calculation method was used to determine the age and growth for E. fasciatus. According to Bhattacharya method eight age group were verified growth parameters L∞=36.75 cm (TL), K= 0.35 year -1 and t0= -0.41121 were derived from Ford and Walford method. The highest range of growth recorded in the first year as it grows about 14.32 cm. Then the rate of growth either slowed down slightly or get closer to being steady.
本文于2006年6月至2007年5月对北红海地区443条石斑鱼(Epinephelus fasciatus)的年龄和生长进行了调查。长度-重量关系的t检验分析显示，b值之间存在显著差异，其中男性为正异速生长，而女性和性别组合为等速生长。“Kc”和(Kn)因子按月研究，并根据体型分类，雌性通常多于雄性。两性合计Kc总平均值为1.428 (SE)。±0.4134)，该值表明状况良好。Kc和Kn值均随鱼体尺寸的增大而增大，直至鱼体尺寸为31.4时均呈下降趋势。另一方面，这两个条件因子在月份之间表现出波动，其中8月份(产卵季节)的值最低。采用反算法测定筋膜棘鱼的年龄和生长情况。根据Bhattacharya法验证8个年龄组的生长参数L∞=36.75 cm (TL)， K= 0.35 year -1和t0= -0.41121由Ford和Walford法得到。最高的生长范围记录在第一年，因为它生长约14.32厘米。然后，增长率要么略微放缓，要么接近稳定。
{"title":"Age and Growth of Epinephelus fasciatus from northern Red Sea","authors":"Mohammed Abozeid, Ashraf Ahmed, M. Alwany, M. El-Sherbiny, Belal Saleh","doi":"10.12816/CAT.2019.28620","DOIUrl":"https://doi.org/10.12816/CAT.2019.28620","url":null,"abstract":"Age and Growth of the fish Epinephelus fasciatus (n=443) from Northern Red Sea were investigated, during the period from June 2006 to May 2007. The t-test analysis for the length-weight relationships showed a significant difference between b values, where male are positive allometric while female and sexes combined are isometric. The \"Kc\" and (Kn) factor was studied monthly and according to size classes, where females usually outnumbered males. The total average of Kc for sexes combined was 1.428 (SE.± 0.4134), this value is indication of a good condition. The value for both Kc and Kn increased in line with increased fish size until the size 31.4, and then decreased in value. On the other hand, both condition factors showed fluctuations among months, where the lowest value was recorded during August (within spawning season). The back calculation method was used to determine the age and growth for E. fasciatus. According to Bhattacharya method eight age group were verified growth parameters L∞=36.75 cm (TL), K= 0.35 year -1 and t0= -0.41121 were derived from Ford and Walford method. The highest range of growth recorded in the first year as it grows about 14.32 cm. Then the rate of growth either slowed down slightly or get closer to being steady.","PeriodicalId":42145,"journal":{"name":"Catrina-The International Journal of Environmental Sciences","volume":"158 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78523283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Metwally R. Kottb, Nadia El-Agroudy, A. Ali, M. Hamed, Heba M. Ezz El-Din
Petroleum hydrocarbons are one of the main pollutants which affected directly on the Gulf of Suez environment due to petroleum products deliveries and production as well as fuel combustion emissions from shipping activities and refineries. There are several Methods for treatment of hydrocarbons such as physical, mechanical, chemical and biological methods. Broth media containing ACF (accommodated fraction), WSF (water soluble fractions) or anthracene were used separately as a sole carbon source. 17 fungal species were isolated from water and sediment of three selected stations (Port-Tawfik, El-Ziaytia and Attaka), then screened in each substrate. Capillary gas chromatography (CGC) analysis used to chemically profiling each substrate after inoculation. Our results showed that, Aspergillus flavus was the most effective of degradation ACF reach to (98.79 %). In case of WSF the isolate Penicillium chrysogenium was the highest percentage of degradation (98.53 %). Anthracene degradation after 2 weeks recorded (56.08 %) by using Cladosporium sp. In conclusion, the Gulf of Suez contain several promising fungal species that could be used in biodegradation of petroleum hydrocarbons as a save alternatives in marine ecosystem.
{"title":"Biodegradation of Some Petroleum Hydrocarbons by Fungi Isolated from Gulf of Suez","authors":"Metwally R. Kottb, Nadia El-Agroudy, A. Ali, M. Hamed, Heba M. Ezz El-Din","doi":"10.12816/CAT.2019.28632","DOIUrl":"https://doi.org/10.12816/CAT.2019.28632","url":null,"abstract":"Petroleum hydrocarbons are one of the main pollutants which affected directly on the Gulf of Suez environment due to petroleum products deliveries and production as well as fuel combustion emissions from shipping activities and refineries. There are several Methods for treatment of hydrocarbons such as physical, mechanical, chemical and biological methods. Broth media containing ACF (accommodated fraction), WSF (water soluble fractions) or anthracene were used separately as a sole carbon source. 17 fungal species were isolated from water and sediment of three selected stations (Port-Tawfik, El-Ziaytia and Attaka), then screened in each substrate. Capillary gas chromatography (CGC) analysis used to chemically profiling each substrate after inoculation. Our results showed that, Aspergillus flavus was the most effective of degradation ACF reach to (98.79 %). In case of WSF the isolate Penicillium chrysogenium was the highest percentage of degradation (98.53 %). Anthracene degradation after 2 weeks recorded (56.08 %) by using Cladosporium sp. In conclusion, the Gulf of Suez contain several promising fungal species that could be used in biodegradation of petroleum hydrocarbons as a save alternatives in marine ecosystem.","PeriodicalId":42145,"journal":{"name":"Catrina-The International Journal of Environmental Sciences","volume":"72 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88525063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Mohamed, M. Elwan, K. El-Hamed, M. Abdel-Salam, A. A. El-Deeb
The present study was conducted during the period 2014-2016 at the greenhouse facilities of the Department of Horticulture, Faculty of Agriculture, Suez Canal University.The experiment tested the effects of growing three daughter plants (ramets) sizes based on crown diameter (large > 7 mm, medium 5-7 mm and small < 5 mm) in combination with three medium types (M1, coco peat + vermiculite + perlite; M2, coco peat + rice hull + sawdust; M3, coco peat + rice hull + rice straw in 1:1:1 v/v) on plug transplant growth in container (84-cell foam trays) of three strawberry cultivars. Results of the main effect of the initial ramet size indicated positive effects of large ramet on obtaining better plug plant vegetative and rooting characteristics than medium or small size ramets. Results of the main effect of medium types showed that both M1 and M2 produced plug plants with better leaf petiole length, leaf fresh weight (FW) and Dry weight (DW) and crown diameter than M3, but root dry mass was not affected by medium types. Plug transplant grown on M3 had better contents of chlorophyll and carotenoids in their leaves than those on M1 or M2. Nutrient analysis of plug plant leaves indicated that plug plants grown on M1 had more P, K, Fe and Cu, and those on M2 had more N, Ca and Mn, while plug plants derived from M3 had more Mg and Zn. Results also indicated that plug transplants were not different in some vegetative and rooting characters among the different strawberry cultivars, especially in number of leaves per transplant, leaves FW and DW, crown DW and root DW, while root length was better in transplants of cv. 'Festival' than 'Tudla' and 'Sweet Charlie'. Leaves of plug plants in cv. 'Tudla' recorded higher chlorophyll and carotenoids than 'Festival' and 'Sweet Charlie' and also had more N, P, and K contents. Generally results indicated that optimum plug transplant production and growth was obtained by the culture of large size ramets in trays filled with any of the tested medium types and cultivars. These results indicated the possibility of producing large number of strawberry plug transplants in Egypt using soilless, suspended growing system as alternative to their production in field nursery to avoid the hazardous effects of soil fumigation and pesticide use.
{"title":"Strawberry Plug Transplant Production in Trays: The Influence of Genotype, Initial Ramet Size and Substrate","authors":"F. Mohamed, M. Elwan, K. El-Hamed, M. Abdel-Salam, A. A. El-Deeb","doi":"10.12816/CAT.2019.28607","DOIUrl":"https://doi.org/10.12816/CAT.2019.28607","url":null,"abstract":"The present study was conducted during the period 2014-2016 at the greenhouse facilities of the Department of Horticulture, Faculty of Agriculture, Suez Canal University.The experiment tested the effects of growing three daughter plants (ramets) sizes based on crown diameter (large > 7 mm, medium 5-7 mm and small < 5 mm) in combination with three medium types (M1, coco peat + vermiculite + perlite; M2, coco peat + rice hull + sawdust; M3, coco peat + rice hull + rice straw in 1:1:1 v/v) on plug transplant growth in container (84-cell foam trays) of three strawberry cultivars. Results of the main effect of the initial ramet size indicated positive effects of large ramet on obtaining better plug plant vegetative and rooting characteristics than medium or small size ramets. Results of the main effect of medium types showed that both M1 and M2 produced plug plants with better leaf petiole length, leaf fresh weight (FW) and Dry weight (DW) and crown diameter than M3, but root dry mass was not affected by medium types. Plug transplant grown on M3 had better contents of chlorophyll and carotenoids in their leaves than those on M1 or M2. Nutrient analysis of plug plant leaves indicated that plug plants grown on M1 had more P, K, Fe and Cu, and those on M2 had more N, Ca and Mn, while plug plants derived from M3 had more Mg and Zn. Results also indicated that plug transplants were not different in some vegetative and rooting characters among the different strawberry cultivars, especially in number of leaves per transplant, leaves FW and DW, crown DW and root DW, while root length was better in transplants of cv. 'Festival' than 'Tudla' and 'Sweet Charlie'. Leaves of plug plants in cv. 'Tudla' recorded higher chlorophyll and carotenoids than 'Festival' and 'Sweet Charlie' and also had more N, P, and K contents. Generally results indicated that optimum plug transplant production and growth was obtained by the culture of large size ramets in trays filled with any of the tested medium types and cultivars. These results indicated the possibility of producing large number of strawberry plug transplants in Egypt using soilless, suspended growing system as alternative to their production in field nursery to avoid the hazardous effects of soil fumigation and pesticide use.","PeriodicalId":42145,"journal":{"name":"Catrina-The International Journal of Environmental Sciences","volume":"32 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76334823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Quail hunting is a major socioeconomic practice in Egyptian coastal areas. The accumulation of cadmium in feathers (chest and tail), muscle, kidneys and livers were analyzed and compared in both wild migratory and domesticated Japanese quails using atomic absorption spectrophotometry. Domesticated Japanese quail recorded higher concentrations of cadmium than the wild one. Cadmium concentration was higher in kidneys and livers than that in the pectoral muscle and feathers. To assess potential risk to human health, daily intake (EDI) and target hazard quotient (THQ) were calculated. The EDI in all tissues was within the permissible tolerable daily intake (PTDI) limit except for kidney values in domesticated species. THQ of all tested organs was less than one, indicating that harmful impacts are not expected. People should avoid eating kidneys and livers of quail in their diets but can safely feed on muscles as they accumulate low percentage of the toxic cadmium.
{"title":"Human Health Risk via Cadmium Concentration in Different Tissues of Domesticated Japanese Quail (Coturnix japonica) and Wild Common Quail (Coturnix coturnix)","authors":"Basma M. Sheta, H. El-Hamid, M. El-Alfy","doi":"10.12816/CAT.2019.28601","DOIUrl":"https://doi.org/10.12816/CAT.2019.28601","url":null,"abstract":"Quail hunting is a major socioeconomic practice in Egyptian coastal areas. The accumulation of cadmium in feathers (chest and tail), muscle, kidneys and livers were analyzed and compared in both wild migratory and domesticated Japanese quails using atomic absorption spectrophotometry. Domesticated Japanese quail recorded higher concentrations of cadmium than the wild one. Cadmium concentration was higher in kidneys and livers than that in the pectoral muscle and feathers. To assess potential risk to human health, daily intake (EDI) and target hazard quotient (THQ) were calculated. The EDI in all tissues was within the permissible tolerable daily intake (PTDI) limit except for kidney values in domesticated species. THQ of all tested organs was less than one, indicating that harmful impacts are not expected. People should avoid eating kidneys and livers of quail in their diets but can safely feed on muscles as they accumulate low percentage of the toxic cadmium.","PeriodicalId":42145,"journal":{"name":"Catrina-The International Journal of Environmental Sciences","volume":"38 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80219506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Today, reverse osmosis membranes are the leading technology for new desalination installations, however, a challenge facing widespread application of RO technology is membrane fouling. In the present study, we used an environmentally friendly green inhibitor as anti-scaling and anti-biofouling in reverse osmosis (RO) desalination plants. The influence of Sargassum sp., Corallina mediterranea, and Avicennia marina on RO membrane mineral scaling was evaluated using gypsum as a model scalant. Antibacterial properties for three marine extracts from Sargassum sp., C. mediterranea, and Avicennia marina were investigated with Gram-positive bacteria (ArthrobactersulfureusYACS14, Staphylococcus aureus) and Gram-negative bacteria (VibrioanguillarumMVM425, Escherichia coli). The antimicrobial results were detected for the two selected extracts as the most potent extracts (ethyl acetate, methanol crude extracts of the Avicennia marina leaves). Data showed that ratios of 3 and 5% recorded the highest suppression percentages (100%) for all tested bacteria including bacterial community collected from Eastern Harbor. On the other side, data confirmed that the anti-scalant properties by 100 ppm of Avicennia marina leave extract giving 85% of scale inhibition. The effect of Avicennia marina leaves extract for calcium sulfate dihydrate (gypsum) scaling on selected reverse osmosis (RO) membrane surfaces was investigated. The effect of different concentrations of Avicennia marina leaves extract was observed in the extent of surface scale coverage and surface crystal size among the membrane studied.
{"title":"Green Inhibitor as Antibacteria and Antiscaling in Reverse Osmosis Desalination Plants","authors":"S. Hamdona, A. El-Aassar, H. A. Ibrahim, A. Ahmed","doi":"10.12816/CAT.2019.28631","DOIUrl":"https://doi.org/10.12816/CAT.2019.28631","url":null,"abstract":"Today, reverse osmosis membranes are the leading technology for new desalination installations, however, a challenge facing widespread application of RO technology is membrane fouling. In the present study, we used an environmentally friendly green inhibitor as anti-scaling and anti-biofouling in reverse osmosis (RO) desalination plants. The influence of Sargassum sp., Corallina mediterranea, and Avicennia marina on RO membrane mineral scaling was evaluated using gypsum as a model scalant. Antibacterial properties for three marine extracts from Sargassum sp., C. mediterranea, and Avicennia marina were investigated with Gram-positive bacteria (ArthrobactersulfureusYACS14, Staphylococcus aureus) and Gram-negative bacteria (VibrioanguillarumMVM425, Escherichia coli). The antimicrobial results were detected for the two selected extracts as the most potent extracts (ethyl acetate, methanol crude extracts of the Avicennia marina leaves). Data showed that ratios of 3 and 5% recorded the highest suppression percentages (100%) for all tested bacteria including bacterial community collected from Eastern Harbor. On the other side, data confirmed that the anti-scalant properties by 100 ppm of Avicennia marina leave extract giving 85% of scale inhibition. The effect of Avicennia marina leaves extract for calcium sulfate dihydrate (gypsum) scaling on selected reverse osmosis (RO) membrane surfaces was investigated. The effect of different concentrations of Avicennia marina leaves extract was observed in the extent of surface scale coverage and surface crystal size among the membrane studied.","PeriodicalId":42145,"journal":{"name":"Catrina-The International Journal of Environmental Sciences","volume":"21 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82134071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohamed Abdel-Sater, N. Hussein, Nashwa A. H. Fetyan, S. Gad
This study was designed to optimize different conditions for improving enzyme production by Penicillium brevicompactum AUMC 10987, and to precipitate and immobilize cellulases by cross-linking method using glutaraldehyde, magnetic nanocarriers and encapsulation using Ca-alginate. Maximum activity of cellulase was achieved in medium containing date palm leaves, sodium nitrate, after 9 days incubation at 30°C and pH 6. Solid state fermentation was more convenient method in cellulase production. After ammonium sulphate precipitation, enzyme activities exceeded two-folded, constructing more stability for the enzyme structure and giving maximum activities at 50℃ and pH 4.8. Hence, precipitated enzyme could work in higher temperature and acidity conditions. The immobilization of cellulases by encapsulation was the most valuable technique retaining the activity of enzyme. Moreover, the immobilized enzyme remained active up to 80°C, giving its maximum activity at 50°C and pH 5.5. Enzyme encapsulated within alginate beads is preferred due to easy for formulation, mild gelation conditions, non-toxic, biocompatibility, low cost and resistance to microbial attack. Magnetic nannocariers immobilized enzyme had opportunity to be reused after 4 cycles/16 hours. The current study could provide a robust and highly valuable immobilized enzyme for different industrial and biotechnological applications.
{"title":"Immobilization of Cellulases Produced by Penicillium brevicompactum AUMC 10987, using Cross-Linkage, Chitosan-Coating and Encapsulation","authors":"Mohamed Abdel-Sater, N. Hussein, Nashwa A. H. Fetyan, S. Gad","doi":"10.12816/CAT.2019.28624","DOIUrl":"https://doi.org/10.12816/CAT.2019.28624","url":null,"abstract":"This study was designed to optimize different conditions for improving enzyme production by Penicillium brevicompactum AUMC 10987, and to precipitate and immobilize cellulases by cross-linking method using glutaraldehyde, magnetic nanocarriers and encapsulation using Ca-alginate. Maximum activity of cellulase was achieved in medium containing date palm leaves, sodium nitrate, after 9 days incubation at 30°C and pH 6. Solid state fermentation was more convenient method in cellulase production. After ammonium sulphate precipitation, enzyme activities exceeded two-folded, constructing more stability for the enzyme structure and giving maximum activities at 50℃ and pH 4.8. Hence, precipitated enzyme could work in higher temperature and acidity conditions. The immobilization of cellulases by encapsulation was the most valuable technique retaining the activity of enzyme. Moreover, the immobilized enzyme remained active up to 80°C, giving its maximum activity at 50°C and pH 5.5. Enzyme encapsulated within alginate beads is preferred due to easy for formulation, mild gelation conditions, non-toxic, biocompatibility, low cost and resistance to microbial attack. Magnetic nannocariers immobilized enzyme had opportunity to be reused after 4 cycles/16 hours. The current study could provide a robust and highly valuable immobilized enzyme for different industrial and biotechnological applications.","PeriodicalId":42145,"journal":{"name":"Catrina-The International Journal of Environmental Sciences","volume":"370 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91123331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present study the ameliorating effects of Si nutrition supplied as 1 mmol L−1 sodium silicate were proved on the seedling growth of canola (Brassica napus L.) seedlings under salinity stress (i.e. 0, 30, 60, 90 and 120 mmol L−1sodium chloride). Salinity obviously disrupted cellular homeostasis, something was evident in the decreased percent of scavenging HO-free radical and Metal chelating %, and hence reduction in photosynthetic pigments contents (chl.a and carotenoids). Silicon nutrition, however, enabled canola cells to balance between the steady-state levels of different ROS through improving the detoxification of the excess ROS. This was prevalent in higher scavenging percent of HO- and metal chelating. In addition, Si maintained membranes integrity through improved levels of lipid peroxidation inhibition %. Si buffered oxidative stress through sustaining the enzymatic and non-enzymatic scavenging capacity of reactive oxygen species in salt-stressed plants. Enzymatic antioxidants such as (CAT, SOD, APX, and POS) activities as well as non-enzymatic antioxidants such as carotenoids contents phenolics contents increased at 120 mmol L−1 sodium chloride. Thus, silicon nutrition alleviated the deleterious effects of salinity on the growth of canola plants through increased reactive oxygen species scavenging capacity (enzymatically and non-enzymatically), maintaining the membrane integrity of seedling cells as evidenced by raising the reducing power contents, sustained higher levels of chlorophyll. Consequently, enhance seedlings growth observations.
{"title":"Ameliorating Effect of Silicon on Growth Vigor, Physiological and biochemical Traits of Salinized Canola Seedlings (Brassica napus L.)","authors":"R. El-Shazoly","doi":"10.12816/cat.2019.28622","DOIUrl":"https://doi.org/10.12816/cat.2019.28622","url":null,"abstract":"In the present study the ameliorating effects of Si nutrition supplied as 1 mmol L−1 sodium silicate were proved on the seedling growth of canola (Brassica napus L.) seedlings under salinity stress (i.e. 0, 30, 60, 90 and 120 mmol L−1sodium chloride). Salinity obviously disrupted cellular homeostasis, something was evident in the decreased percent of scavenging HO-free radical and Metal chelating %, and hence reduction in photosynthetic pigments contents (chl.a and carotenoids). Silicon nutrition, however, enabled canola cells to balance between the steady-state levels of different ROS through improving the detoxification of the excess ROS. This was prevalent in higher scavenging percent of HO- and metal chelating. In addition, Si maintained membranes integrity through improved levels of lipid peroxidation inhibition %. Si buffered oxidative stress through sustaining the enzymatic and non-enzymatic scavenging capacity of reactive oxygen species in salt-stressed plants. Enzymatic antioxidants such as (CAT, SOD, APX, and POS) activities as well as non-enzymatic antioxidants such as carotenoids contents phenolics contents increased at 120 mmol L−1 sodium chloride. Thus, silicon nutrition alleviated the deleterious effects of salinity on the growth of canola plants through increased reactive oxygen species scavenging capacity (enzymatically and non-enzymatically), maintaining the membrane integrity of seedling cells as evidenced by raising the reducing power contents, sustained higher levels of chlorophyll. Consequently, enhance seedlings growth observations.","PeriodicalId":42145,"journal":{"name":"Catrina-The International Journal of Environmental Sciences","volume":"16 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88485631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Clinical infectious diseases are a major public health concerns worldwide, particularly in developing countries, where access to health care is extremely limited. In a context of increased development of antibiotic-resistant microorganisms, extensive research efforts, to explore novel antimicrobial from newly isolated organisms, are in need. Therefore, our study was carried out to isolate actinobacteria, for the first time, from two different soils collected from Cameroon. Fifty-eight out of 81 isolates of actinobacteria showed antimicrobial activity against human pathogens based on agar disc overlay bioassay. Meanwhile, the excreted metabolites of these actinobacteria were also tested for antimicrobial activity using agar disc diffusion test. Nine Gram-positive and negative human bacterial pathogens were used. One fungal pathogen was also tested. Our results showed that the most active actinobacterial isolates are belonging to the genera: Microtetraspora sp. (CSU3), Nocardiopsis sp. (CSU5), Streptomyces sp. (PSP3, PSU28 and PSU30), and Micromonospora sp. (CSD50). Streptomyces sp. and Actinomadura sp. isolates were the most dominant genera producing potent antimicrobial secondary metabolites, indicating that Streptomycetes-phenotype isolates are providing high quality metabolites for drug discovery purposes. Natural occurring biochemical compounds excreted in liquid media were also tested and proved the presence of alkaloids and tannins compounds.
{"title":"Pharmaceutically active secondary metabolites of newly isolated actinobacteria from two Cameroonian soils","authors":"Steve Takoukam Toukam, A. Ngakou, S. Mansour","doi":"10.12816/CAT.2019.27395","DOIUrl":"https://doi.org/10.12816/CAT.2019.27395","url":null,"abstract":"Clinical infectious diseases are a major public health concerns worldwide, particularly in developing countries, where access to health care is extremely limited. In a context of increased development of antibiotic-resistant microorganisms, extensive research efforts, to explore novel antimicrobial from newly isolated organisms, are in need. Therefore, our study was carried out to isolate actinobacteria, for the first time, from two different soils collected from Cameroon. Fifty-eight out of 81 isolates of actinobacteria showed antimicrobial activity against human pathogens based on agar disc overlay bioassay. Meanwhile, the excreted metabolites of these actinobacteria were also tested for antimicrobial activity using agar disc diffusion test. Nine Gram-positive and negative human bacterial pathogens were used. One fungal pathogen was also tested. Our results showed that the most active actinobacterial isolates are belonging to the genera: Microtetraspora sp. (CSU3), Nocardiopsis sp. (CSU5), Streptomyces sp. (PSP3, PSU28 and PSU30), and Micromonospora sp. (CSD50). Streptomyces sp. and Actinomadura sp. isolates were the most dominant genera producing potent antimicrobial secondary metabolites, indicating that Streptomycetes-phenotype isolates are providing high quality metabolites for drug discovery purposes. Natural occurring biochemical compounds excreted in liquid media were also tested and proved the presence of alkaloids and tannins compounds.","PeriodicalId":42145,"journal":{"name":"Catrina-The International Journal of Environmental Sciences","volume":"14 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73792932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The evaluation of hepatoprotective effect of Mustard seed (MS) extract was assessed against paracetamol induced hepatic injury in albino rats. Animals were divided into 4 groups, each of 5 rats. Group I served as control group; group II treated with ethanolic extract from Mustard seed at a dose level of 500 mg/kg body weight orally once for 10 days. Group III represents induced liver injury animals using single dose of paracetamol (PCT). Group IV combined treatment of MS extract and PCT. Elevated serum marker enzymes such as ALT, AST, ALP, GGT and LDH were observed due to paracetamol treatment. Combined administration of MS extract with PCT to rat partially normalized the altered hepatic biochemical markers caused by PCT. Moreover, PCT caused histological changes in livers of rat including swelling and dilation in sinusoidal space, inflammation in portal tract and hepatocytes, as well as, hyperplasia in Kuppfer cells. However, co-administration of MS extract with PCT alleviated to some extent the changes caused by PCT but not as the normal liver. The results of this study strongly indicate that Mustard seed has possible potent hepatoprotective action against paracetamol induced hepatic damage in rats.
{"title":"Possible Hepatoprotective Effects of Mustard Seed Extract Against Paracetamol-Induced Liver Injury in Male Albino Rat","authors":"Howayda E. Khaled","doi":"10.21608/cat.2018.14337","DOIUrl":"https://doi.org/10.21608/cat.2018.14337","url":null,"abstract":"The evaluation of hepatoprotective effect of Mustard seed (MS) extract was assessed against paracetamol induced hepatic injury in albino rats. Animals were divided into 4 groups, each of 5 rats. Group I served as control group; group II treated with ethanolic extract from Mustard seed at a dose level of 500 mg/kg body weight orally once for 10 days. Group III represents induced liver injury animals using single dose of paracetamol (PCT). Group IV combined treatment of MS extract and PCT. Elevated serum marker enzymes such as ALT, AST, ALP, GGT and LDH were observed due to paracetamol treatment. Combined administration of MS extract with PCT to rat partially normalized the altered hepatic biochemical markers caused by PCT. Moreover, PCT caused histological changes in livers of rat including swelling and dilation in sinusoidal space, inflammation in portal tract and hepatocytes, as well as, hyperplasia in Kuppfer cells. However, co-administration of MS extract with PCT alleviated to some extent the changes caused by PCT but not as the normal liver. The results of this study strongly indicate that Mustard seed has possible potent hepatoprotective action against paracetamol induced hepatic damage in rats.","PeriodicalId":42145,"journal":{"name":"Catrina-The International Journal of Environmental Sciences","volume":"11 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87498391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study attempts to carry out a critical revision for the genus Ephedra in Egypt based on morpholo-gical characters for leaf, stem and flower. DNA sequencing data used for the first time for DNA barcoding of the family Ephedraceae in Egypt. One chloroplast marker-intergenic spacer trn-H/psb-A and another nuclear Internal Transcribed Spacer (ITS) were sequenced to authenticate the identification and to reconstruct the phylogenetic relationships of the Egyptian Ephedra species. The results revealed that, the name of Ephedra ciliata Fischer and C. A. Mey., was illegitimate and became a synonym to Ephedra foliata Boiss., which reported here as accepted name. The current work recommends using the DNA barcode as a tool for species identification of Ephedra species as well as other gymnosperms in Egypt.
{"title":"Taxonomic, DNA Barcoding and Phylogenetic Reassessment of the Egyptian Ephedra L. (Ephedraceae)","authors":"A. Faried, A. El-Banhawy, Mesfer Elqahtani","doi":"10.12816/CAT.2018.14292","DOIUrl":"https://doi.org/10.12816/CAT.2018.14292","url":null,"abstract":"This study attempts to carry out a critical revision for the genus Ephedra in Egypt based on morpholo-gical characters for leaf, stem and flower. DNA sequencing data used for the first time for DNA barcoding of the family Ephedraceae in Egypt. One chloroplast marker-intergenic spacer trn-H/psb-A and another nuclear Internal Transcribed Spacer (ITS) were sequenced to authenticate the identification and to reconstruct the phylogenetic relationships of the Egyptian Ephedra species. The results revealed that, the name of Ephedra ciliata Fischer and C. A. Mey., was illegitimate and became a synonym to Ephedra foliata Boiss., which reported here as accepted name. The current work recommends using the DNA barcode as a tool for species identification of Ephedra species as well as other gymnosperms in Egypt.","PeriodicalId":42145,"journal":{"name":"Catrina-The International Journal of Environmental Sciences","volume":"48 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75642473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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