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Novel Quinoline Derivatives as Antitumor Agents Against HepG2 Cells: Synthesis, Characterization, In Silico, In Vitro and Docking Studies 新型喹啉衍生物作为HepG2细胞的抗肿瘤药物:合成、表征、硅、体外和对接研究
IF 0.3 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2020-04-01 DOI: 10.21608/cat.2020.85762
M. Soliman, I. Ali, S. El-Sakka, Omayma El-Sayed Abdel-Basset Mohamed
New quinoline derivatives based on allyl and amino acid were prepared and characterized using elemental analysis (CHN%), HNMR, CNMR spectra. The theoretical calculations which carried out using different computer programs permit proposing an optimized geometry for the formed complexes. The molecular modeling for some representative compounds were evaluated and discussed. The energy of the HOMO and LUMO was calculated and assessed. The most stable structure of the synthesized compounds was suggested and evaluated its energy. The most benefit properties, which play very important role in drug synthesis referred to the surface properties of the compounds, were evaluated and discussed. The application of the DFT, on the target compounds, gave promising properties required for antitumor drugs. Docking of the synthesized compounds with HepG2-code: 5EQG protein, as liver carcinoma cell, gave promising inhibition in silico level. The antitumor activity of the target compounds in vitro level gave activity with some compounds exceeded the market drug.
合成了基于烯丙基和氨基酸的新型喹啉衍生物,并用元素分析(CHN%)、HNMR、CNMR光谱对其进行了表征。使用不同的计算机程序进行的理论计算允许为形成的复合物提出优化的几何形状。对一些代表性化合物的分子模型进行了评价和讨论。计算并评价了HOMO和LUMO的能量。提出了合成化合物的最稳定结构,并对其能量进行了评价。对化合物的表面性质进行了评价和讨论,这些性质在药物合成中起着非常重要的作用。DFT在目标化合物上的应用,为抗肿瘤药物提供了有希望的特性。合成的化合物与HepG2-code: 5EQG蛋白对接,作为肝癌细胞,在硅水平上具有良好的抑制作用。目标化合物的抗肿瘤活性在体外水平上与部分化合物的活性超过了市场上的药物。
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引用次数: 3
Effect of Exogenous Application of 24-Epibrassinosteroids and Hydrogen Peroxide on Some Biochemical Characteristics of Cuminum cyminum L. grown under Drought Stress 外源施用24-表油菜素内酯和过氧化氢对干旱胁迫下小茴香部分生化特性的影响
IF 0.3 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2020-04-01 DOI: 10.21608/cat.2020.85788
H. Abbaspour, N. Roudbari, K. M. Kalantari, A. Aien
To evaluate the responses of cumin plant to different levels of drought stress with exogenous application of either 24-epibrassinolide (24-EBL) or hydrogen peroxide (H2O2) and a mixture of them, a factorial experiment was conducted, in a complete randomized design with three replicas, at the experimental greenhouse. Treatments included three levels of drought stress (100%, 75%, and 50% of FC) in which at fifth week after germination (beginning of reproductive growth) application of H2O2 (0, 0.5 and 1mM) and 24-epibrassinosteroid (0, 0.5 and 1mM) at two stages, first 3 days before applying the then, 15 days after. The results showed that with increasing stress intensity, the amounts of proline, malondialdehyde and superoxide dismutase (SOD) enzyme increased, but decreased the total protein and catalase enzyme in the root. The results of interaction between drought stress and exogenous application of 24-EBL showed the highest total protein content (11.30 mg/l) at 1mM 24-EBL under moderate stress of 75% of FC. Also, the highest of proline content of the root and shoot were obtained (10.15 and 10.91 mMol gFW –l , respectively) under severe stress (50% FC) and spraying with 1mM 24EBL, respectively. However, a decrease in MDA content, with the application of 24-EBL e and H2O2, was recorded. In general, it can report that drought stress reduced the efficiency of photosynthesis and plant production. Moreover, application of 24-EBL and H2O2 was able to improve the photosynthetic system and resistance of the cumin plant to stress.
为了评价外源施用24-表油菜素内酯(24-EBL)或过氧化氢(H2O2)及其混合物对孜然植株不同程度干旱胁迫的响应,采用完全随机设计、3个重复的析因试验。在萌发后第5周(生殖生长开始)施用H2O2(0、0.5和1mM)和24-表油菜素内酯(0、0.5和1mM),分2个阶段处理,分别在前3天和后15天施用。结果表明:随着胁迫强度的增加,根中脯氨酸、丙二醛和超氧化物歧化酶(SOD)含量增加,总蛋白和过氧化氢酶含量降低;干旱胁迫与外源施加24-EBL的交互作用结果表明,在75% FC的中度胁迫下,1mM 24-EBL的总蛋白含量最高(11.30 mg/l)。重度胁迫(50% FC)和1mM 24EBL喷施下,根和茎部脯氨酸含量最高,分别为10.15和10.91 mMol gFW -l。然而,在24-EBL e和H2O2的处理下,MDA含量有所下降。总的来说,可以报道干旱胁迫降低了光合作用效率和植物产量。此外,施用24-EBL和H2O2能改善孜然植株的光合系统,提高其抗逆性。
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引用次数: 2
Ecological Study on some Grasses Growing Naturally in the Deltaic Mediterranean Coast of Egypt 埃及地中海三角洲沿岸天然草类的生态学研究
IF 0.3 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2019-09-01 DOI: 10.12816/CAT.2019.49168
I. Mashaly, Sekina M Ayaad, M. El-Shamy, Esraa A Elsoudy
The present study aims to investigate the floristic composition and vegetation analysis of the floraassociated with some grasses growing naturally on the sand formations in the Deltaic Mediterranean coastof Egypt. The total number of the recorded plant species was 79 species (44 annuals and 35 perennials)belonging to 70 genera and 26 families. The life –form spectra of the recorded species comprised 44therophytes, 13 cryptophytes, eight hemicryptophytes, seven chamaephytes and phanerphytes for each.Chorologically, the Saharo-Sindian element was represented by relatively high number of species (34),followed by Irano-Turanian (32 species), then Euro-Siberian (10 species) and Sudano-Zambezian (9species). Based on the importance alue of each species, the classification of the recorded species in 36sampled stands led to recognition of four vegetation groups (A-D). The characteristics dominant andcodominant species of vegetation groups were Elymus farcatus and Calligonum polygonoides (A),Hordeum murinum (B), Stipagrostis lanata and Echinops spinosus (C) and Lycium schweinfurthii andEchinops spinosus (D). DCA diagram showed overlapping between the identified groups. CCA-biplotexhibited that sulfates, calcium, potassium, magnesium and calcium carbonate were the most effectivesoil variables controlling the abundance of identified vegetation groups.
本研究旨在研究埃及地中海三角洲沿岸沙层上自然生长的几种禾本科植物的区系组成和植被分析。共记录植物种类79种(一年生44种,多年生35种),隶属于26科70属。所记录物种的生命形态谱包括44种有生植物、13种隐植物、8种半隐植物、7种变生植物和显生植物。从地理分布上看,撒哈拉- sinindian区物种较多(34种),其次是伊朗-图拉尼亚区(32种)、欧洲-西伯利亚区(10种)和苏丹-赞比西亚区(9种)。根据每个物种的重要性值,对36个样地的记录物种进行分类,可识别出4个植被类群(A-D)。植被类群的优势种和共优势种分别为:羊角草(Elymus farcatus)和蓼(caligonum polygonoides, A)、鼠耳草(Hordeum murinum, B)、刺蒺藜(Stipagrostis lanata)和刺棘(Echinops spinosus, C)、枸杞(Lycium schweinfurthii)和刺棘(Echinops spinosus, D)。cca - biplod结果表明,硫酸盐、钙、钾、镁和碳酸钙是控制已鉴定植被群丰度的最有效的石油变量。
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引用次数: 1
RAPD-PCR analysis and gene expression of CYP450 in Tribolium castaneum adults in response to different insecticides 不同杀虫剂对木栗成虫CYP450基因表达的影响及RAPD-PCR分析
IF 0.3 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2019-09-01 DOI: 10.21608/cat.2019.47459
S. Mahmoud, A. Kamel
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引用次数: 2
Docking Applications For Some Novel Complexes Derived From Quinoline Derivatives 喹啉衍生物衍生的新型配合物的对接应用
IF 0.3 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2019-09-01 DOI: 10.21608/CAT.2019.48667
I. Ali, S. El-Sakka, M. Soliman, O. Mohamed
The newquinoline derivatives: 2-oxo-1,2-dihydroquinoline-4-carbohydrazide (1),2-(allyloxy)quinoline-4-carbohydrizde (2), 1-allyl-2-oxo-1,2-dihydroquinoline-4-carbohydrazid (3) and 2-(allyl-thio)quinoline-4-carbohydrazide (4)and their Cu(II), Ni(II) and Co(II) complexes were synthesized and characterized by using elemental analysis (CHNM%), FTIR, UV/Vis, 1HNMR, 13CNMRspectra, DTA, TGA, magnetic susceptibility and the conductivity of 0.001 M in DMSO. The FTIRspectra of the synthesized ligands and their complexes were giving the characteristic stretching vibration bands. The weight losswhich appeared in the TG analysis indicates that, there are different types of water molecules in the formed complexes. The theoretical calculations which carried out using different computer programs permit proposing an optimized geometry for the formed complexes. The molecular modeling for the free ligands and their complexes were evaluated and discussed. Docking of the synthesized compounds with HepG2-code:5EQG protein, as liver carcinoma cell, gave promising inhibition in Silico level.The antimicrobial activity of the target compounds with E. Coli, B. Subtils and Asp. Niger,in Vitro level, gave promising result. The interaction of the compounds with the microorganisms was tested in Silico level. E. Coli was used as an example for the target microorganism. The protein which used for docking process was 5C9T.
合成了新的喹啉衍生物:2-氧-1,2-二氢喹啉-4-碳肼(1)、2-(烯丙氧基)喹啉-4-碳肼(2)、1-烯丙基-2-氧-1,2-二氢喹啉-4-碳肼(3)和2-(烯丙基-硫代)喹啉-4-碳肼(4)及其Cu(II)、Ni(II)和Co(II)配合物,并利用元素分析(CHNM%)、FTIR、UV/Vis、1HNMR、13cnmr光谱、差热分析、热重分析、磁化率和DMSO中0.001 M电导率对其进行了表征。合成的配体及其配合物的红外光谱显示出特有的伸缩振动带。热重分析中出现的失重现象表明,形成的配合物中存在不同类型的水分子。使用不同的计算机程序进行的理论计算允许为形成的复合物提出优化的几何形状。对游离配体及其配合物的分子模拟进行了评价和讨论。合成的化合物与HepG2-code:5EQG蛋白对接,作为肝癌细胞,在硅水平上具有良好的抑制作用。目的化合物对大肠杆菌、枯草芽孢杆菌和Asp的抑菌活性。在体外水平,尼日尔给出了令人满意的结果。在硅水平上测试了化合物与微生物的相互作用。以大肠杆菌为例进行目标微生物研究。用于对接过程的蛋白为5C9T。
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引用次数: 0
Anti-microbial and Anti-diabetic Activity of Six Seaweeds Collected from the Red Sea, Egypt 6种产自埃及红海的海藻的抗微生物和抗糖尿病活性
IF 0.3 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2019-09-01 DOI: 10.12816/CAT.2019.49157
N. Osman, Adel A. Siam, Islam M. El-Manawy, Y. Jeon
Marine algae are a well-known source of active compounds with many biological activities. Many studieshad point to the anti-microbial and anti-diabetic activity of seaweeds. Egyptian shores are rich withseaweeds, yet studies concerning their biological activity are inadequate. In the present work, the 80%methanolic extract of six seaweeds (Actinotrichia fragilis, Cystoseira myrica, Hormophysa cuneiformis,Laurencia papillosa, Sargassum cinereum, and Turbinaria turbinate) were tested for their antimicrobialactivity using disc diffusion method and anti-diabetic activity using the Inhibition of α-glucosidasemethod. The six species were collected from Hurghada, Red Sea, Egypt during late December, 2012. Theresults should that the algal extracts were effective against both Gram-positive and Gram-negative bacteriawhen used at 2000μg/disc concentration. On Candida albicuns, both low and high concentrations showedactivity. For anti-diabetic activity, H. cuneiformis was the most active species that reached to 53%inhibition of α-glucosidase at the highest concentration (1000 μg/ml) with IC50 676.9 μg/ml. Inconclusion, the tested seaweeds possess a good anti-microbial and anti-diabetic activity, especially thespecies Hormophysa cuneiformis.
众所周知,海藻是具有多种生物活性的活性化合物的来源。许多研究指出海藻具有抗微生物和抗糖尿病的活性。埃及海岸有丰富的海藻,但关于其生物活性的研究还不充分。采用圆盘扩散法和α-葡萄糖苷抑制法对6种海藻(脆弱放线菌、杨梅囊藻、齿形藻、乳突藻、马尾藻和鼻甲藻)80%甲醇提取物的抗菌活性和抗糖尿病活性进行了研究。6种于2012年12月下旬在埃及红海赫尔格达采集。结果表明,当浓度为2000μg/盘时,藻提取物对革兰氏阳性菌和革兰氏阴性菌均有较好的抑菌效果。对白色念珠菌,低浓度和高浓度均表现出活性。在抗糖尿病活性方面,楔形杉对α-葡萄糖苷酶的抑制率最高(1000 μg/ml),达到53%,IC50为676.9 μg/ml。综上所述,所测海藻具有良好的抗微生物和抗糖尿病活性,尤其是楔形藻。
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引用次数: 10
Biodiversity and habitat use of wintering and breeding waterbirds in Burullus Lake (Ramsar site), Egypt 埃及布鲁勒斯湖(拉姆萨尔遗址)越冬和繁殖水鸟的生物多样性和栖息地利用
IF 0.3 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2019-09-01 DOI: 10.12816/CAT.2019.49179
Basma M. Sheta
The present study seeks to assess the bird biodiversity at the Important Bird Area (IBA) and Ramsar site,Lake Burullus, northern Egypt. Waterbirds biodiversity was assessed at 14 selected sites in the lake duringwinter and spring seasons. The assessment included richness, composition, evenness, abundance, andSimpson and Shannon-Wiener diversity indices. Bird species diversity varied spatially and temporallyamong different sites during the study period. Estimated species richness was 49 species. Winter birdswere mainly migratory waterbirds like waterfowl, distributed in the islets for foraging, which was the mainactivity of 51% of total birds, followed by 36% roosting and 13% breeding at the lake. Evidence ofbreeding was recorded, such as collecting nest material, courtship behavior and food collecting forjuveniles. The hierarchical cluster analysis separated the lake sites into four distinctive habitats: lakeshores, open water, islets and reed beds. Seven bird species showed significant affinities with differenthabitat types: Laughing dove (Streptopelia senegalensis) favored the lake shores habitat (P< 0.04); Littlebittern (Ixobrychus minutus) (P< 0.04), Pied kingfisher (Ceryle rudis) (P< 0.04), Common kingfisher(Alcedo atthis) (P< 0.02), Pied wagtail (Motacilla alba) (P< 0.04), and Graceful prinina (Prinia gracilis)(P< 0.01) were found in islands habitat. Little egret (Egretta garzetta) dominated the reed beds habitatgroup (P< 0.03). The results reflected the importance of Burullus Lake as a wintering and breeding habitatfor birds and suggested that more implemented conservation and management strategies should beadopted.
本研究旨在评估埃及北部Burullus湖重要鸟类区(IBA)和Ramsar遗址的鸟类生物多样性。在冬春两季,选取湖泊14个地点对水鸟生物多样性进行了评估。评价指标包括丰富度、组成、均匀度、丰度、simpson和Shannon-Wiener多样性指数。研究期间不同地点鸟类物种多样性存在时空差异。物种丰富度估计为49种。冬季鸟类以水禽等迁徙水鸟为主,主要在岛上觅食,占总鸟类的51%,其次为栖息,13%在湖中繁殖。记录了繁殖的证据,如收集筑巢材料,求偶行为和为幼鸟收集食物。分层聚类分析将湖泊遗址分为四种不同的栖息地:湖岸、开阔水域、小岛和芦苇床。7种鸟类与不同生境类型有显著的亲缘关系:笑鸽(Streptopelia senegalensis)偏好湖滨生境(P< 0.04);海岛生境主要有小麻鸦(Ixobrychus minutus) (P< 0.04)、花斑翠鸟(Ceryle rudis) (P< 0.04)、普通翠鸟(Alcedo atthis) (P< 0.02)、花斑鹡尾(Motacilla alba) (P< 0.04)和花斑金鸡(Prinia gracilis)(P< 0.01)。芦苇床生境组以小白鹭(Egretta garzetta)为主(P< 0.03)。研究结果反映了布鲁勒斯湖作为鸟类越冬和繁殖栖息地的重要性,并建议采取更有效的保护和管理策略。
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引用次数: 2
On the Ecology of Weeds in the Orchards habitat During Summer Season in Nile Delta, Egypt 埃及尼罗河三角洲夏季果园生境杂草生态学研究
IF 0.3 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2019-09-01 DOI: 10.12816/CAT.2019.47548
I. Mashaly, Sami Al-Barti, A. A. El-Gawad
The present study aims to investigate the ecology of weed communities associated with some orchards habitat using the variations in environmental factors to determine the distribution and abundance of the recognized weed communities in the study areas. The sampled stands were distributed in four Governorates. Field visits were started from January 2014 to September 2015, 45 stands were selected for sampling the vegetation types during summer season. The application of TWINSPAN classification based on the importance values of 49 plant species recorded in selected 45 sampled stands representing the summer orchards sites in the study areas led to the recognition of four vegetation groups (Euphorbia heterophylla, Commelina benghalensis, Cynanchum acutum and Xanthium strumarium were the dominate plants). In summer season, the application of DCA technique proved that, the plant communities recognized by TWINSPAN classification were markedly distinguishable and having clear pattern of segregation on the ordination plane. The application of CCA technique was used to determine the most effective soil variables controlling the abundance and distribution of weed communities associated during summer season.
本研究旨在利用环境因子的变化,研究与某些果园生境相关的杂草群落生态学,以确定研究区已识别的杂草群落的分布和丰度。抽样的林分分布在四个省。2014年1月至2015年9月开展实地考察,选取45个林分进行夏季植被类型采样。采用TWINSPAN分类方法,对研究区45个夏季果园样地的49种植物的重要度进行分类,识别出4个植被类群,优势植物为大戟(Euphorbia heterophylla)、金合欢(Commelina benghalensis)、金合欢(Cynanchum acutum)和苍耳(Xanthium strumarium)。在夏季,DCA技术的应用证明,TWINSPAN分类识别的植物群落具有明显的可区分性,在排序平面上具有明显的分离模式。应用CCA技术确定了控制夏季杂草群落丰度和分布的最有效土壤变量。
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引用次数: 0
Vegetation Ecology Associated with Some Species of Family Zygophyllaceae in Different Regions of Egyptian Desert 埃及沙漠不同地区槐科植物的植被生态学研究
IF 0.3 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2019-09-01 DOI: 10.12816/CAT.2018.6083.1008
I. Mashaly
The Zygophyllaceae is a widespread family of common existence in arid and semi-arid habitats of the Old and New Worlds, especially in seasonally dry deserts. Various members of this family are characterized by high efficient potential for the treatment of broad spectrum health disorders and well known in scientific and folk literature for their medicinal uses. TWINSPAN cluster for the 38 stands yielded four vegetation groups (A, B, C and D) at the 3rd level of classification. These groups were identified after the first dominant species that attained the highest importance value (IV). Group A: Zygophyllum aegyptium, group B: Fagonia cretica, group C: Zygophyllum coccineum and group D: Pulicaria undulata. The vegetation group A characterized sand formation habitats along Deltaic Mediterranean coastal desert, while group B represented roadside habitats of the western Mediterranean coastal desert, group C dominated Wadi Hagul bed and gravel desert habitats of Suez. Furthermore, group D inhabited wadi bed and roadside habitats in Ismailia. Ordination of the vegetation groups along 2 axes was performed using Detrended Correspondence Analysis (DCA), while Canonical Correspondence Analysis (CCA) was utilized to determine the relation between the community types and soil factors.
赤藓科是一个分布广泛的科,普遍存在于新旧世界的干旱和半干旱栖息地,特别是在季节性干旱的沙漠中。该家族的各种成员都具有治疗广谱健康障碍的高效潜力,在科学和民间文献中因其药用用途而闻名。38个林分的TWINSPAN聚类在3级分类中得到4个植被组(A、B、C和D)。在第一个优势种达到最高重要值(IV)后进行分类:A组:埃及柴叶(Zygophyllum aegyptium), B组:柴叶(Fagonia cretica), C组:尾骨柴叶(Zygophyllum coccineum), D组:波状轮(Pulicaria undulata)。A组植被以地中海三角洲滨海沙漠成沙生境为主,B组植被以地中海西部滨海沙漠路边生境为主,C组植被以哈古勒河河床和苏伊士砾石沙漠生境为主。此外,D组居住在伊斯梅利亚的河床和路边栖息地。利用非趋势对应分析(DCA)对2个轴上的植被类群进行排序,利用典型对应分析(CCA)确定群落类型与土壤因子的关系。
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引用次数: 3
Comments on the Egyptian species of Ephedra L. (Ephedraceae) 埃及麻黄属植物(麻黄科)的评述
IF 0.3 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2019-09-01 DOI: 10.12816/CAT.2019.47458
H. Freitag
The article deals critically with several aspects of the recently published article on the Egyptian species of genus Ephedra, published by Faried et al. 2018, in Catrina 17. It provides additional and correcting data on the morphology and distribution of some species including an identification key based on vegetative characters, detailed distribution maps based on the evaluation of material from all relevant herbaria and photos of the respective species taken in the field.
本文批判性地讨论了最近发表的关于埃及麻黄属物种的文章的几个方面,该文章由fared等人于2018年发表在《卡特里娜17》上。它提供了一些物种形态和分布的额外和校正数据,包括基于营养特征的识别密钥,基于所有相关植物标本室材料评估的详细分布图以及在野外拍摄的各自物种的照片。
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引用次数: 0
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Catrina-The International Journal of Environmental Sciences
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