Pub Date : 1900-01-01DOI: 10.7324/jabb.2022.10s109
V. Yadav, R. C. Yadav, P. Choudhary, S. Sharma, Neeta Bhagat
Drought as an environmental stressor poses threat to crop yields and consequently jeopardizes agricultural sustainability. Microbes harboring in the roots of native plants having ability to promote plant growth can offer a promising tool to combat the drought stress in plants. In this context, drought tolerant Bacillus strains were isolated from the rhizospheric soils from hot arid regions of Jaisalmer district of Rajasthan, India. On screening 120 isolates, two isolates DT-85 and DT-97 identified as Bacillus paramycoides and Bacillus paranthracis, respectively, using 16S rRNA gene sequencing, showed tolerance to high osmotic stress (10–30% polyethylene glycol 6000), salinity (5–15%), and temperature (45°C). Strain DT-97 exhibited efficient plant growth promoting traits such as production of (1) phosphate, (2) siderophores (SPI 2.93), (3) exopolysaccharide (216–244%), (4) indole-3-acetic acid (49–59%), (5) ammonia, and (6) gibberellic acid (110.5%) in comparison to strain DT-85 under both normal watered and drought stress conditions. Drought stress conditions reduced the root-shoot length, leaf area, and chlorophyll content of wheat crops on inoculation of B. paramycoides DT-85 and B. paranthracis DT-97 resulted in mitigation of drought stress by enhanced production of drought combating molecules like superoxide dismutase, peroxidase, catalase and proline. B. paranthracis DT-97 showed better plant growth promoting attributes and thus can be a used as a bioinoculant for mitigating drought in wheat crop.
{"title":"Mitigation of drought stress in wheat (Triticum aestivum L.) by inoculation of drought tolerant Bacillus paramycoides DT-85 and Bacillus paranthracis DT-97","authors":"V. Yadav, R. C. Yadav, P. Choudhary, S. Sharma, Neeta Bhagat","doi":"10.7324/jabb.2022.10s109","DOIUrl":"https://doi.org/10.7324/jabb.2022.10s109","url":null,"abstract":"Drought as an environmental stressor poses threat to crop yields and consequently jeopardizes agricultural sustainability. Microbes harboring in the roots of native plants having ability to promote plant growth can offer a promising tool to combat the drought stress in plants. In this context, drought tolerant Bacillus strains were isolated from the rhizospheric soils from hot arid regions of Jaisalmer district of Rajasthan, India. On screening 120 isolates, two isolates DT-85 and DT-97 identified as Bacillus paramycoides and Bacillus paranthracis, respectively, using 16S rRNA gene sequencing, showed tolerance to high osmotic stress (10–30% polyethylene glycol 6000), salinity (5–15%), and temperature (45°C). Strain DT-97 exhibited efficient plant growth promoting traits such as production of (1) phosphate, (2) siderophores (SPI 2.93), (3) exopolysaccharide (216–244%), (4) indole-3-acetic acid (49–59%), (5) ammonia, and (6) gibberellic acid (110.5%) in comparison to strain DT-85 under both normal watered and drought stress conditions. Drought stress conditions reduced the root-shoot length, leaf area, and chlorophyll content of wheat crops on inoculation of B. paramycoides DT-85 and B. paranthracis DT-97 resulted in mitigation of drought stress by enhanced production of drought combating molecules like superoxide dismutase, peroxidase, catalase and proline. B. paranthracis DT-97 showed better plant growth promoting attributes and thus can be a used as a bioinoculant for mitigating drought in wheat crop.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115446421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.7324/jabb.2023.110213
J. Prashanth, H. Manjunatha
Varying atmospheric temperatures invariably induce thermal stress in the silkworm, Bombyx mori , which influence the functionality of aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Thus, we have investigated AST and ALT activity in the new bivoltine lines (NBL). Interestingly, day-3 fifth instar larvae of NBL-5 being more susceptible to the thermal stress of 45°C showed highest level of AST (11.64 µ m/mL) and ALT (9.83 µ m/mL) activity in their hemolymph. Consequently, on day-5, AST (3.54 µ m/mL) and ALT (1.39 µ m/mL) activity were lower than day-3. Comparatively, between NBL and its parental breeds, the AST and ALT activity were found to be higher in day-3 (12.41 and 10.90 µ m/mL) and day-5 (4.02 and 1.69 µ m/mL) larvae of CSR 2 , while it was 12.93 and 10.14 µ m/mL in CSR 27 on day-3, and 4.31 and 2.03 µ m/mL on day-5, respectively. This salient finding validates the linkage between the rate of cytotoxicity and AST and ALT enzyme activity in relation to the thermotolerance in NBL and its parents. Thus, the silkworm larvae can be considered as a model system for rapid evaluation of cytotoxicity and varied levels of AST and ALT activity as one of the biomarkers to correlate well with the tolerance level to critical temperature.
{"title":"Transaminases activity in the hemolymph: Biomarkers determining the thermal stress in the new bivoltine lines of Bombyx mori.","authors":"J. Prashanth, H. Manjunatha","doi":"10.7324/jabb.2023.110213","DOIUrl":"https://doi.org/10.7324/jabb.2023.110213","url":null,"abstract":"Varying atmospheric temperatures invariably induce thermal stress in the silkworm, Bombyx mori , which influence the functionality of aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Thus, we have investigated AST and ALT activity in the new bivoltine lines (NBL). Interestingly, day-3 fifth instar larvae of NBL-5 being more susceptible to the thermal stress of 45°C showed highest level of AST (11.64 µ m/mL) and ALT (9.83 µ m/mL) activity in their hemolymph. Consequently, on day-5, AST (3.54 µ m/mL) and ALT (1.39 µ m/mL) activity were lower than day-3. Comparatively, between NBL and its parental breeds, the AST and ALT activity were found to be higher in day-3 (12.41 and 10.90 µ m/mL) and day-5 (4.02 and 1.69 µ m/mL) larvae of CSR 2 , while it was 12.93 and 10.14 µ m/mL in CSR 27 on day-3, and 4.31 and 2.03 µ m/mL on day-5, respectively. This salient finding validates the linkage between the rate of cytotoxicity and AST and ALT enzyme activity in relation to the thermotolerance in NBL and its parents. Thus, the silkworm larvae can be considered as a model system for rapid evaluation of cytotoxicity and varied levels of AST and ALT activity as one of the biomarkers to correlate well with the tolerance level to critical temperature.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125061932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.7324/jabb.2023.111162
S. D., Seema J. Patel, Anil H. Shyam Mohan
{"title":"Isolation and identification of killer yeasts from Psidium gujava","authors":"S. D., Seema J. Patel, Anil H. Shyam Mohan","doi":"10.7324/jabb.2023.111162","DOIUrl":"https://doi.org/10.7324/jabb.2023.111162","url":null,"abstract":"","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126004689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Arsenic contamination in the food chain: A threat to food security and human health","authors":"Siril Singh, Rajni Yadav, Sheenu Sharma, A. Singh","doi":"10.7324/jabb.2023.69922","DOIUrl":"https://doi.org/10.7324/jabb.2023.69922","url":null,"abstract":"","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123272428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.7324/jabb.2023.110113
Shubhankar Barua, Anusree Hait, Gourab Shome, Naveduddin Nesar, S. Barua
Groundwater contamination by geogenic arsenic (As) is globally a significant health risk. The availability of As in groundwater as well as its toxicity depends on its oxidation state. Metal reducing bacteria in groundwater-associated aquifer sediments can reduce sediment boundless toxic arsenate (AsV) more toxic arsenite (AsIII) and mobilize them into groundwater. Under anaerobic conditions as prevalent in deep surface sediments, dissimilatory arsenate-respiring prokaryotes are the predominant arsenate-reducing microbial members. However, in anaerobic environments of subsurface sediments of lesser depth, microbial As cycling, and their mobilization are rarely studied. In rural parts of India and Bangladesh, open defecation, use of pit latrines, and soak pits are standard practices, and seepage of fecal matter, as well as coliforms into adjoining sediments, could be of high possibility. Herein, distribution of aquifer sediment-associated arsenate tolerant microbial population in general and coliforms (including fecal coliforms) in particular from shallower depths were studied. The difference in the recovery of total arsenate-resistant microbial count under aerobic and anaerobic conditions was evident. Prevalence of fecal coliforms, specifically Escherichia coli (revealed by molecular characterization), was observed at greater depths due to their facultative anaerobic nature.
{"title":"A study on coliforms in arsenic-contaminated aquifer sediments of Chakdah, West Bengal, India","authors":"Shubhankar Barua, Anusree Hait, Gourab Shome, Naveduddin Nesar, S. Barua","doi":"10.7324/jabb.2023.110113","DOIUrl":"https://doi.org/10.7324/jabb.2023.110113","url":null,"abstract":"Groundwater contamination by geogenic arsenic (As) is globally a significant health risk. The availability of As in groundwater as well as its toxicity depends on its oxidation state. Metal reducing bacteria in groundwater-associated aquifer sediments can reduce sediment boundless toxic arsenate (AsV) more toxic arsenite (AsIII) and mobilize them into groundwater. Under anaerobic conditions as prevalent in deep surface sediments, dissimilatory arsenate-respiring prokaryotes are the predominant arsenate-reducing microbial members. However, in anaerobic environments of subsurface sediments of lesser depth, microbial As cycling, and their mobilization are rarely studied. In rural parts of India and Bangladesh, open defecation, use of pit latrines, and soak pits are standard practices, and seepage of fecal matter, as well as coliforms into adjoining sediments, could be of high possibility. Herein, distribution of aquifer sediment-associated arsenate tolerant microbial population in general and coliforms (including fecal coliforms) in particular from shallower depths were studied. The difference in the recovery of total arsenate-resistant microbial count under aerobic and anaerobic conditions was evident. Prevalence of fecal coliforms, specifically Escherichia coli (revealed by molecular characterization), was observed at greater depths due to their facultative anaerobic nature.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114453948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.7324/jabb.2023.120550
Javeria Uzma, S. Talla, P. Mamidala
{"title":"Insights into the impact of spermidine in reducing salinity stress in Gerbera jamesonii","authors":"Javeria Uzma, S. Talla, P. Mamidala","doi":"10.7324/jabb.2023.120550","DOIUrl":"https://doi.org/10.7324/jabb.2023.120550","url":null,"abstract":"","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114474275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.7324/jabb.2023.113474
J. Yomso, Sandeep Menon, Mustapha Na-Allah Sale, Johnson Yumnam
Mung bean being a leguminous crop is helpful in improving the soil properties. Since it is a pulse crop, it is deprived of proper fertilization. Hence, the study was aimed to determine the best fertilizer combination and cropping system for mung bean production. The experiment comprised eight treatments and three replications arranged in a split-plot design. The results revealed that yield components, namely, number of pods/plant, number of seeds/pod, 1000 seed weight, stover yield, seed yield, and biological yield in sole mung bean were significantly higher when mung bean is grown as an intercrop. While in the case of different fertilizer levels, the treatment with the combination of nanofertilizers and NPK fertilizers produced higher yield attributes than all other treatments. In contrast, the control (no fertilizer) produced lower yield characters. The study concludes that the combination of 50% recommended dose fertilizer + 50% nano NPK fertilizers + sole mung bean produced the highest yield and can be used for higher mung bean production.
{"title":"Performance of mung bean as influenced by different levels of fertilizers and cropping systems in the semi-arid region of India","authors":"J. Yomso, Sandeep Menon, Mustapha Na-Allah Sale, Johnson Yumnam","doi":"10.7324/jabb.2023.113474","DOIUrl":"https://doi.org/10.7324/jabb.2023.113474","url":null,"abstract":"Mung bean being a leguminous crop is helpful in improving the soil properties. Since it is a pulse crop, it is deprived of proper fertilization. Hence, the study was aimed to determine the best fertilizer combination and cropping system for mung bean production. The experiment comprised eight treatments and three replications arranged in a split-plot design. The results revealed that yield components, namely, number of pods/plant, number of seeds/pod, 1000 seed weight, stover yield, seed yield, and biological yield in sole mung bean were significantly higher when mung bean is grown as an intercrop. While in the case of different fertilizer levels, the treatment with the combination of nanofertilizers and NPK fertilizers produced higher yield attributes than all other treatments. In contrast, the control (no fertilizer) produced lower yield characters. The study concludes that the combination of 50% recommended dose fertilizer + 50% nano NPK fertilizers + sole mung bean produced the highest yield and can be used for higher mung bean production.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129808986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Histopathologically, amyloids are known as β -sheet rich extracellular protein deposits that are generally associated with neuro-degenerative diseases of mammals including α -synuclein observed in Parkinson’s disease (PD). Microorganisms have been reported for production of similar proteins with functional physiological traits also termed as “functional amyloids” helping them in invasion, biofilm-formation, host-colonization
{"title":"Effects of gut bacteria and their amyloids on mental health and neurodegeneration in Parkinson’s disease","authors":"Kush Mehta, Radhika R. Bhat, Anoop R. Markande","doi":"10.7324/jabb.2023.16293","DOIUrl":"https://doi.org/10.7324/jabb.2023.16293","url":null,"abstract":"Histopathologically, amyloids are known as β -sheet rich extracellular protein deposits that are generally associated with neuro-degenerative diseases of mammals including α -synuclein observed in Parkinson’s disease (PD). Microorganisms have been reported for production of similar proteins with functional physiological traits also termed as “functional amyloids” helping them in invasion, biofilm-formation, host-colonization","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128432787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.7324/jabb.2023.110117
V. Jha, A. Bhosale, Prakruti Kapadia, Agraj Bhargava, Arpita Marick, Zahra Charania, Omkar Parulekar, Mafiz Shaikh, Bhakti Madaye
Hepatocellular carcinoma (HCC) is the fourth major cause of death worldwide, with a global diminishing survival rate of 19%. Irrespective of the advanced therapeutic strategies against this carcinoma, it persists as one of the most challenging diseases. Moreover, the low efficacy of existing treatment stratagem using synthetic drugs against HCC has led to the urgent investigation of natural alternatives that can result in a more efficient treatment with fewer health side effects than their synthetic counterparts. In this study, a total of 1259 phytochemicals were docked against 25 potential HCC protein targets with the help of PyRx, a virtual screening tool software. The pharmacokinetics and drug-like properties of these chemicals were examined through SWISS ADME webserver. Based on their binding affinity against each protein target, only 250 ligands were shortlisted further for toxicity analysis using the web tools ADMETlab 2.0 and Protox II. In accordance with the bioavailability radar and pharmacokinetic profile analysis, only two non-toxic phytochemicals: Sorgolactone and Alectrol, emerged as the most befitting drug candidates against HCC protein targets 6HH1 and 1ZXM, respectively. The findings of this study suggest that these two phytochemicals can be explored and exploited further for their use as potential HCC drug candidates.
{"title":"Multitargeted molecular docking study of phytochemicals on hepatocellular carcinoma","authors":"V. Jha, A. Bhosale, Prakruti Kapadia, Agraj Bhargava, Arpita Marick, Zahra Charania, Omkar Parulekar, Mafiz Shaikh, Bhakti Madaye","doi":"10.7324/jabb.2023.110117","DOIUrl":"https://doi.org/10.7324/jabb.2023.110117","url":null,"abstract":"Hepatocellular carcinoma (HCC) is the fourth major cause of death worldwide, with a global diminishing survival rate of 19%. Irrespective of the advanced therapeutic strategies against this carcinoma, it persists as one of the most challenging diseases. Moreover, the low efficacy of existing treatment stratagem using synthetic drugs against HCC has led to the urgent investigation of natural alternatives that can result in a more efficient treatment with fewer health side effects than their synthetic counterparts. In this study, a total of 1259 phytochemicals were docked against 25 potential HCC protein targets with the help of PyRx, a virtual screening tool software. The pharmacokinetics and drug-like properties of these chemicals were examined through SWISS ADME webserver. Based on their binding affinity against each protein target, only 250 ligands were shortlisted further for toxicity analysis using the web tools ADMETlab 2.0 and Protox II. In accordance with the bioavailability radar and pharmacokinetic profile analysis, only two non-toxic phytochemicals: Sorgolactone and Alectrol, emerged as the most befitting drug candidates against HCC protein targets 6HH1 and 1ZXM, respectively. The findings of this study suggest that these two phytochemicals can be explored and exploited further for their use as potential HCC drug candidates.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129694060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.7324/jabb.2023.110221
Rachmi Putri, Prasetya Anugerah Gusti, N. Wijayanti
Begomovirus infects various species of the Solanaceae family and reduces crop productivity. It is important to note that Bemisia tabaci is a vector that contributes to increasing infection. Therefore, this study aims to identify and modify DNA extraction methods, as well as detect the presence of Begomovirus in whitefly and eggplants ( Solanum melongena L .) plantations. S. melongena leaves showing infection symptoms were collected from eggplant cultivation in Rejodani, Sleman, D.I. Yogyakarta, Indonesia. The identification of whitefly collected from the leaves was performed microscopically, DNA extraction was carried out using lysis and cetyltrimethylammonium bromide (CTAB) buffer, while the presence of Begomovirus was detected using polymerase chain reaction. Based on the results, the whitefly is a part of Bemisia genus, the DNA quantification showed that Bemisia spp. has low purity, while S. melongena is slightly purer. However, Begomovirus presence was detected in both sample since the DNA band appeared in ±580 bp. It was concluded that DNA from Begomovirus in the whitefly Bemisia spp. and S. melongena can be extracted using lysis and CTAB buffer, respectively. It was also shown that Begomovirus infects S. melongena similarly to its vector, Bemisia spp., in Rejodani eggplant plantations.
begomavirus感染茄科的多种植物并降低作物产量。重要的是要注意，烟粉虱是一种媒介，有助于增加感染。因此，本研究旨在确定和改进DNA提取方法，并检测白蝇和茄子(Solanum melongena L .)种植园中begomvirus的存在。在印度尼西亚日惹省Sleman地区的茄子栽培中采集了显示感染症状的茄蚜叶片。采用显微镜下鉴定白蝇，采用裂解法和十六烷基三甲基溴化铵(CTAB)缓冲液提取DNA，采用聚合酶链反应检测begomvirus是否存在。结果表明，该粉虱属于粉虱属，DNA定量分析表明，粉虱属纯度较低，而黑绒粉虱纯度稍高。但两份样本均检测到begomavirus存在，DNA条带出现在±580 bp。结论:用裂解法和CTAB缓冲液分别可提取白蝇和黑蝇的begomvirus DNA。结果还表明，Begomovirus在茄子园内感染的葡萄球菌与其载体Bemisia spp.相似。
{"title":"Begomovirus detection in the whitefly Bemisia spp. on eggplant Solanum melongena L. leaves","authors":"Rachmi Putri, Prasetya Anugerah Gusti, N. Wijayanti","doi":"10.7324/jabb.2023.110221","DOIUrl":"https://doi.org/10.7324/jabb.2023.110221","url":null,"abstract":"Begomovirus infects various species of the Solanaceae family and reduces crop productivity. It is important to note that Bemisia tabaci is a vector that contributes to increasing infection. Therefore, this study aims to identify and modify DNA extraction methods, as well as detect the presence of Begomovirus in whitefly and eggplants ( Solanum melongena L .) plantations. S. melongena leaves showing infection symptoms were collected from eggplant cultivation in Rejodani, Sleman, D.I. Yogyakarta, Indonesia. The identification of whitefly collected from the leaves was performed microscopically, DNA extraction was carried out using lysis and cetyltrimethylammonium bromide (CTAB) buffer, while the presence of Begomovirus was detected using polymerase chain reaction. Based on the results, the whitefly is a part of Bemisia genus, the DNA quantification showed that Bemisia spp. has low purity, while S. melongena is slightly purer. However, Begomovirus presence was detected in both sample since the DNA band appeared in ±580 bp. It was concluded that DNA from Begomovirus in the whitefly Bemisia spp. and S. melongena can be extracted using lysis and CTAB buffer, respectively. It was also shown that Begomovirus infects S. melongena similarly to its vector, Bemisia spp., in Rejodani eggplant plantations.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130311160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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