Objective: This study aimed to compared the effects of different placement techniques to the sealing quality of mineral triokside aggregate (MTA) apical plugs at apexification technique by micro-computed tomography (micro-CT) and compared the bond strength to root dentin of an injectable MTA (BIOfactor MTA), MTA Angelus and AH Plus. Methods: Sixty dentinal root slices were obtained from 20 maxillary centrals.A canal-like hole was drilled into each slices canal space.The samples were divided into 3 groups (n=20).All materials were delivered into the holes. Push-out tests were performed and fracture types were analysed with a strereomicroscope. In the second part of the study,72 maxillary central teeth with standardised artificial divergent open apex were divided into 4 groups; MTA Angelus and BIOfactor MTA were mixed mechanically, and introduced to form 4 mm thick apical plugs by hand condensation or indirect-ultrasonic activation for 10 seconds. Incidence of external voids between dentin walls and MTA apical plugs and porosity inside MTA were determined by volumetric analysis with micro-CT. Results: No significantly difference was found between the bond strength values of the materials (p:0.370; p>0.05).The external voids and porous voids are similar in both MTA (p: 0.685; p>0.05).When indirect-ultrasonic activation was applied,there was significantly less porosity statistically than hand condensation (p:0.00; p<0.05). Conclusion: MTA Angelus and BIOfactor MTA materials showed similar results in terms of bond strength to root dentin, fracture types, adaptation to dentin walls and structural porosity rate. Both MTA materials showed less structural porosity when placed by indirect ultrasonic activation technique compared to manual condensation.
研究目的本研究旨在通过显微计算机断层扫描(micro-CT)技术,比较不同放置技术对三钾矿骨料(MTA)根尖塞封闭质量的影响,并比较可注射 MTA(BIOfactor MTA)、MTA Angelus 和 AH Plus 与根部牙本质的粘结强度。方法从 20 个上颌正中获取 60 个牙本质根切片,在每个切片的根管间隙钻一个管状孔,将样本分为 3 组(n=20)。进行推出试验,并用螺旋显微镜分析断裂类型。在研究的第二部分,72 颗具有标准化人工分歧开放顶点的上颌中牙被分为 4 组;MTA Angelus 和 BIOfactor MTA 以机械方式混合,并通过手动冷凝或间接超声激活 10 秒钟的方式导入以形成 4 毫米厚的顶端塞。牙本质壁和 MTA 根尖塞之间的外部空隙发生率以及 MTA 内部的孔隙率是通过显微 CT 的体积分析确定的。结果:两种材料的粘接强度值无明显差异(p:0.370; p>0.05),两种 MTA 的外部空隙和多孔空隙相似(p:0.685; p>0.05)。结论MTA Angelus 和 BIOfactor MTA 材料在与根部牙本质的粘结强度、断裂类型、对牙本质壁的适应性和结构孔隙率方面表现出相似的结果。与人工冷凝相比,两种 MTA 材料在使用间接超声活化技术时的结构孔隙率都较低。
{"title":"Micro-Computed Tomographic Evaluation of the Sealing Quality and Bond Strength of Different MTA Apical Plugs","authors":"Taibe Tokgöz Kaplan, M. Botsalı","doi":"10.58600/eurjther1919","DOIUrl":"https://doi.org/10.58600/eurjther1919","url":null,"abstract":"Objective: This study aimed to compared the effects of different placement techniques to the sealing quality of mineral triokside aggregate (MTA) apical plugs at apexification technique by micro-computed tomography (micro-CT) and compared the bond strength to root dentin of an injectable MTA (BIOfactor MTA), MTA Angelus and AH Plus. Methods: Sixty dentinal root slices were obtained from 20 maxillary centrals.A canal-like hole was drilled into each slices canal space.The samples were divided into 3 groups (n=20).All materials were delivered into the holes. Push-out tests were performed and fracture types were analysed with a strereomicroscope. In the second part of the study,72 maxillary central teeth with standardised artificial divergent open apex were divided into 4 groups; MTA Angelus and BIOfactor MTA were mixed mechanically, and introduced to form 4 mm thick apical plugs by hand condensation or indirect-ultrasonic activation for 10 seconds. Incidence of external voids between dentin walls and MTA apical plugs and porosity inside MTA were determined by volumetric analysis with micro-CT. Results: No significantly difference was found between the bond strength values of the materials (p:0.370; p>0.05).The external voids and porous voids are similar in both MTA (p: 0.685; p>0.05).When indirect-ultrasonic activation was applied,there was significantly less porosity statistically than hand condensation (p:0.00; p<0.05). Conclusion: MTA Angelus and BIOfactor MTA materials showed similar results in terms of bond strength to root dentin, fracture types, adaptation to dentin walls and structural porosity rate. Both MTA materials showed less structural porosity when placed by indirect ultrasonic activation technique compared to manual condensation.","PeriodicalId":42642,"journal":{"name":"European Journal of Therapeutics","volume":"99 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139174470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The Activity Questionnaire for Adults and Adolescents (AQuAA) is used to evaluate physical activity (PA) levels in different age groups. Its validity and reliability in the Turkish language have not been studied yet. This study aims to adapt the AQuAA into Turkish and to investigate its validity and reliability. Methods: A total of 124 volunteers were included in the study. After the Turkish adaptation of AQuAA, the AQuAA-Tr version was administered to the volunteers for test-retest reliability twice, with an interval of two weeks, and the International Physical Activity Questionnaire Short Form (IPAQ-SF) for criterion validity. For construct validity, the step counts of the volunteers were followed for two weeks with the Samsung Health® smartphone pedometer application. The reliability of the AQuAA-Tr was evaluated with intra-class correlation coefficients (ICC). Spearman correlation coefficients (r) were used to analyze the relationships between continuous variables. Results: A total of 72 adolescents (51 females and 21 males, mean age 14.5 ± 0.1 years) and 52 young adults (32 females and 20 males, mean age 25.8 ± 1.3 years) participated in the test-retest reliability and criterion validity study. Thirty-four adolescents (26 female, 8 male, mean age 14.7 ± 0.2 years) and 39 young adults (27 female, 12 male, mean age 25.6 ± 1.5 years) were included in the construct validity study. The test-retest reliability of the questionnaire was in the range of strong to very strong (ICC = 0.704 to 0.982) in adolescents and moderate to strong (ICC = 0.606 to 0.851) in adults for different levels of PA. In the context of the criterion validity, although there were moderate to strong correlations (r = 0.413 to 0.768) between some PA levels of the IPAQ-SF and AQuAA-Tr in adolescents and moderate correlations (r = 0.422 to 0.525) in adults, the correlations were mostly weak or negligible. In relation to construct validity, although there were moderate correlations (r = 0.435 to 0.504) between the Samsung Health® data and some PA levels of the AQuAA-Tr in adults, the correlations were mostly weak or negligible. There were no correlations between the Samsung Health® data and AQuAA-Tr in adolescents. Conclusion: The reliability of the AQuAA-Tr was confirmed in both adolescents and adults. However, the criterion and construct validity of the AQuAA-Tr were not confirmed for either adolescents or adults. Introducing a PA questionnaire, which can provide detailed information about sedentary, light, moderate, and vigorous PA scores separately and total PA scores and allows the evaluation of PA in different categories, into our language is considered beneficial. Yet, the results of AQuAA-Tr should be interpreted carefully in the clinic.
{"title":"Cross-cultural Adaptation of the Activity Questionnaire for Adults and Adolescents into Turkish and Investigation of its Validity and Reliability","authors":"Akın Süzer, Özlem Çinar Özdemir","doi":"10.58600/eurjther1898","DOIUrl":"https://doi.org/10.58600/eurjther1898","url":null,"abstract":"Objective: The Activity Questionnaire for Adults and Adolescents (AQuAA) is used to evaluate physical activity (PA) levels in different age groups. Its validity and reliability in the Turkish language have not been studied yet. This study aims to adapt the AQuAA into Turkish and to investigate its validity and reliability.\u0000Methods: A total of 124 volunteers were included in the study. After the Turkish adaptation of AQuAA, the AQuAA-Tr version was administered to the volunteers for test-retest reliability twice, with an interval of two weeks, and the International Physical Activity Questionnaire Short Form (IPAQ-SF) for criterion validity. For construct validity, the step counts of the volunteers were followed for two weeks with the Samsung Health® smartphone pedometer application. The reliability of the AQuAA-Tr was evaluated with intra-class correlation coefficients (ICC). Spearman correlation coefficients (r) were used to analyze the relationships between continuous variables.\u0000Results: A total of 72 adolescents (51 females and 21 males, mean age 14.5 ± 0.1 years) and 52 young adults (32 females and 20 males, mean age 25.8 ± 1.3 years) participated in the test-retest reliability and criterion validity study. Thirty-four adolescents (26 female, 8 male, mean age 14.7 ± 0.2 years) and 39 young adults (27 female, 12 male, mean age 25.6 ± 1.5 years) were included in the construct validity study. The test-retest reliability of the questionnaire was in the range of strong to very strong (ICC = 0.704 to 0.982) in adolescents and moderate to strong (ICC = 0.606 to 0.851) in adults for different levels of PA. In the context of the criterion validity, although there were moderate to strong correlations (r = 0.413 to 0.768) between some PA levels of the IPAQ-SF and AQuAA-Tr in adolescents and moderate correlations (r = 0.422 to 0.525) in adults, the correlations were mostly weak or negligible. In relation to construct validity, although there were moderate correlations (r = 0.435 to 0.504) between the Samsung Health® data and some PA levels of the AQuAA-Tr in adults, the correlations were mostly weak or negligible. There were no correlations between the Samsung Health® data and AQuAA-Tr in adolescents.\u0000Conclusion: The reliability of the AQuAA-Tr was confirmed in both adolescents and adults. However, the criterion and construct validity of the AQuAA-Tr were not confirmed for either adolescents or adults. Introducing a PA questionnaire, which can provide detailed information about sedentary, light, moderate, and vigorous PA scores separately and total PA scores and allows the evaluation of PA in different categories, into our language is considered beneficial. Yet, the results of AQuAA-Tr should be interpreted carefully in the clinic.","PeriodicalId":42642,"journal":{"name":"European Journal of Therapeutics","volume":" 37","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138964439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Melike Taşci, Z. Fazlıoğulları, B. Ulusoy, M. Durmaz, Vedat Uslu, N. Unver Dogan, A. Karabulut
Objective: The presence of an ethmomaxillary sinus (EMS) may increase the susceptibility to inflammatory paranasal sinus diseases such as chronic rhinosinusitis (CRS) and cause difficulties in surgical interventions to the paranasal sinuses. Therefore, this study aimed to examine the EMS in patients with and without CRS. Methods: The study included 150 patients (300 sides) diagnosed with CRS by the ear–nose–throat clinic and 151 individuals (302 sides) without CRS. Paranasal sinus computed tomography images were reviewed retrospectively. The presence of an EMS (bilateral or not) and its relationship with age and sex were examined. The severity of CRS was determined with the Lund–Mackay scoring system, and its relationship with EMS was evaluated. Results: The EMS was detected in 7 patients (7/301, 2.32%) and 9 sides (9/602, 1.49%) of 301 patients (602 sides) included. The incidence in the CRS group was 2.6%. Three cases were unilateral, and one was bilateral. The incidence in the control group was 1.98%, two cases were unilateral, and one was bilateral. According to the Lund–Mackay scoring system, the mean CRS severity was 8.62 (±5.47). Its severity was 5.25 (±3.94) in the EMS group and 8.71 (±5.48) in the non-EMS group. Conclusion: No statistically significant difference was found between the groups with and without CRS in terms of the presence of EMS (p = 0.723). No evidence reveals that EMS increased the severity of CRS.
{"title":"Imaging of the Ethmomaxillary Sinus, its Prevalence, and Evaluation of its Relationship with Chronic Rhinosinusitis","authors":"Melike Taşci, Z. Fazlıoğulları, B. Ulusoy, M. Durmaz, Vedat Uslu, N. Unver Dogan, A. Karabulut","doi":"10.58600/eurjther1891","DOIUrl":"https://doi.org/10.58600/eurjther1891","url":null,"abstract":"Objective: The presence of an ethmomaxillary sinus (EMS) may increase the susceptibility to inflammatory paranasal sinus diseases such as chronic rhinosinusitis (CRS) and cause difficulties in surgical interventions to the paranasal sinuses. Therefore, this study aimed to examine the EMS in patients with and without CRS. Methods: The study included 150 patients (300 sides) diagnosed with CRS by the ear–nose–throat clinic and 151 individuals (302 sides) without CRS. Paranasal sinus computed tomography images were reviewed retrospectively. The presence of an EMS (bilateral or not) and its relationship with age and sex were examined. The severity of CRS was determined with the Lund–Mackay scoring system, and its relationship with EMS was evaluated. Results: The EMS was detected in 7 patients (7/301, 2.32%) and 9 sides (9/602, 1.49%) of 301 patients (602 sides) included. The incidence in the CRS group was 2.6%. Three cases were unilateral, and one was bilateral. The incidence in the control group was 1.98%, two cases were unilateral, and one was bilateral. According to the Lund–Mackay scoring system, the mean CRS severity was 8.62 (±5.47). Its severity was 5.25 (±3.94) in the EMS group and 8.71 (±5.48) in the non-EMS group. Conclusion: No statistically significant difference was found between the groups with and without CRS in terms of the presence of EMS (p = 0.723). No evidence reveals that EMS increased the severity of CRS.","PeriodicalId":42642,"journal":{"name":"European Journal of Therapeutics","volume":"19 6","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dear Editor, Anostomotic stricture (AS) is seen in 2-30% of patients after colorectal surgery. Various factors such as tissue ischaemia, anastomotic leakage and radiotherapy have been suggested in its pathogenesis [1,2]. Endoscopic methods (balloon dilatation, bougie dilatation, stents, endoscopic electroincision), digital dilatation, surgical methods (stapler stricturoplasty, transanal circular stapler resection, transabdominal redo-anastomosis) and corticosteroids are used in AS [3,4]. Surgery is generally preferred in complete/near complete AS [1]. Stents; the benefit of stents in AS after oncological surgery has not been shown [3]. However, Philip BC Pangg et al. treated near-total AS non-operatively with the colonic/rectal endoscopic ultrasound (EUS) anastomosis technique and a hot lumen metallic stent [5]. We applied 3-stage bougie dilation to the patient with near complete anastomotic stricture. A 59-year-old female patient who underwent laparoscopic anterior resection due to sigmoid colon tumor was followed up with complaints of abdominal swelling, intermittent abdominal pain and difficulty in defecation. One month later, when colonoscopy was performed, near complete anastomotic stricture was observed. Bougie dilatation was performed with maloney flexible bougie dilators under wire guidance. Bougie dilatation was performed 3 times with fifteen days intervals. After the first (33, 36 and 42 F) and the second bougie dilatation (36, 42 F), the upper segment of the anastomotic stricture was reached by gastroscopy. After dilatation with a bougie (42, 45 F) for the third time, the colonoscope was easily passed through the anastomosis line to the upper segment. Six months later, colonoscopy was performed and the proximal part of the anastomosis was easily passed without the use of bougie dilators. Balloon dilatation is the first method used in AS. However, several repetitions are necessary for the success of the procedure. In addition, the risk of perforation increases when the stricture diameter is <5 mm and length >1 cm. The chances of success in AS are lower compared to bougie dilatation. Endoscopic electroincision is recommended in failure of balloon dilatation. Digital dilatation: used in distal anorectal anostamotic strictures. Corticosteroid application: very large studies are not available. Bougie dilatation in AS provides tactile feedback, allowing the amount of resistance to the passage of the dilator to be estimated and perforation to be avoided. Bougie dilatation method is simple, inexpensive and low risk of complications. Especially Maloney flexible silicone bougie minimise the risk of complications. Bougie dilatators can remain intact for many years and can be reused. But balloon dilatators are not reused. Surgical methods are used in 3-4% (complete/near complete AS) in the failure of endoscopic methods. But mortality risk is high [1,4-6]. Therefore, the alternative method of Philip BC Pang et al. can be applied [5]. However, due t
亲爱的编辑,2-30%的结直肠手术后患者会出现吻合口狭窄(AS)。其发病机制有多种因素,如组织缺血、吻合口漏和放疗等[1,2]。强直性脊柱炎可采用内窥镜方法(球囊扩张术、球囊扩张术、支架、内窥镜电切术)、数字扩张术、外科手术方法(订书机狭窄成形术、经肛门环形订书机切除术、经腹重新吻合术)和皮质类固醇[3,4]。一般来说,完全/接近完全强直性脊柱炎患者首选手术治疗[1]。支架;肿瘤手术后使用支架治疗强直性脊柱炎的益处尚未得到证实[3]。不过,Philip BC Pangg 等人采用结肠/直肠内镜超声(EUS)吻合技术和热腔金属支架,非手术治疗了近完全性强直性脊柱炎[5]。我们对吻合口近乎完全狭窄的患者采用了三段式套管扩张术。一位 59 岁的女性患者因乙状结肠肿瘤接受了腹腔镜前切除术,随访时主诉腹部肿胀、间歇性腹痛和排便困难。一个月后,在进行结肠镜检查时,发现吻合口几乎完全狭窄。在导丝引导下,使用马洛尼柔性套管扩张器进行了套管扩张术。小口扩张术共进行了 3 次,每次间隔 15 天。第一次(33、36 和 42 F)和第二次(36、42 F)扩张后,通过胃镜检查可以看到吻合口狭窄的上段。第三次使用扩张器(42、45 F)扩张后,结肠镜很容易通过吻合口线到达上段。六个月后进行结肠镜检查,吻合口的近端部分在没有使用扩张器的情况下也很容易通过。球囊扩张是强直性脊柱炎患者首先使用的方法。不过,要想手术成功,必须重复几次。此外,当狭窄直径达到 1 厘米时,穿孔的风险就会增加。与套管扩张术相比,强直性脊柱炎的成功几率较低。如果球囊扩张失败,建议采用内窥镜电切术。数字扩张术:用于远端肛门直肠肛门狭窄。皮质类固醇应用:尚无大型研究。强直性脊柱炎的套管扩张术可提供触觉反馈,从而估计扩张器通过的阻力,避免穿孔。扩张器扩张法操作简单、费用低廉、并发症风险低。尤其是马洛尼柔性硅胶扩张器,可将并发症的风险降至最低。套管扩张器可以保持多年完好无损,可以重复使用。但球囊扩张器不能重复使用。在内窥镜方法失败的情况下,3-4%(完全/接近完全 AS)的患者会采用手术方法。但死亡率很高[1,4-6]。因此,可以采用 Philip BC Pang 等人的替代方法[5]。然而,由于内镜超声检查(EUS)中心有限,在吻合口狭窄的情况下,可以使用可弯曲的扩张器进行逐步扩张。您真诚的
{"title":"Alternative Approach in Colorectal Anastomotic Stricture: Bougie Dilatation","authors":"A. K. Taşkın","doi":"10.58600/eurjther1920","DOIUrl":"https://doi.org/10.58600/eurjther1920","url":null,"abstract":"Dear Editor, Anostomotic stricture (AS) is seen in 2-30% of patients after colorectal surgery. Various factors such as tissue ischaemia, anastomotic leakage and radiotherapy have been suggested in its pathogenesis [1,2]. Endoscopic methods (balloon dilatation, bougie dilatation, stents, endoscopic electroincision), digital dilatation, surgical methods (stapler stricturoplasty, transanal circular stapler resection, transabdominal redo-anastomosis) and corticosteroids are used in AS [3,4]. Surgery is generally preferred in complete/near complete AS [1]. Stents; the benefit of stents in AS after oncological surgery has not been shown [3]. However, Philip BC Pangg et al. treated near-total AS non-operatively with the colonic/rectal endoscopic ultrasound (EUS) anastomosis technique and a hot lumen metallic stent [5]. We applied 3-stage bougie dilation to the patient with near complete anastomotic stricture. A 59-year-old female patient who underwent laparoscopic anterior resection due to sigmoid colon tumor was followed up with complaints of abdominal swelling, intermittent abdominal pain and difficulty in defecation. One month later, when colonoscopy was performed, near complete anastomotic stricture was observed. Bougie dilatation was performed with maloney flexible bougie dilators under wire guidance. Bougie dilatation was performed 3 times with fifteen days intervals. After the first (33, 36 and 42 F) and the second bougie dilatation (36, 42 F), the upper segment of the anastomotic stricture was reached by gastroscopy. After dilatation with a bougie (42, 45 F) for the third time, the colonoscope was easily passed through the anastomosis line to the upper segment. Six months later, colonoscopy was performed and the proximal part of the anastomosis was easily passed without the use of bougie dilators. Balloon dilatation is the first method used in AS. However, several repetitions are necessary for the success of the procedure. In addition, the risk of perforation increases when the stricture diameter is <5 mm and length >1 cm. The chances of success in AS are lower compared to bougie dilatation. Endoscopic electroincision is recommended in failure of balloon dilatation. Digital dilatation: used in distal anorectal anostamotic strictures. Corticosteroid application: very large studies are not available. Bougie dilatation in AS provides tactile feedback, allowing the amount of resistance to the passage of the dilator to be estimated and perforation to be avoided. Bougie dilatation method is simple, inexpensive and low risk of complications. Especially Maloney flexible silicone bougie minimise the risk of complications. Bougie dilatators can remain intact for many years and can be reused. But balloon dilatators are not reused. Surgical methods are used in 3-4% (complete/near complete AS) in the failure of endoscopic methods. But mortality risk is high [1,4-6]. Therefore, the alternative method of Philip BC Pang et al. can be applied [5]. However, due t","PeriodicalId":42642,"journal":{"name":"European Journal of Therapeutics","volume":"378 ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139173995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: One of the most studied topics in electronic apex locators (EALs) is the effect of root canal condition on the accuracy of EALs. In this study, the accuracy of Root ZX Mini, Raypex 6, and Apex ID in detecting root perforation was evaluated in a dry environment and in the presence of saline, ethylenediamine tetraacetic acid (EDTA), and sodium hypochlorite (NaOCl) solutions. Methods: The mesiobuccal roots of 64 human maxillary first molars were selected for the study. These root canals were perforated from buccal root surface using a #1 Freze Beutherlock Peeso to form a 0.4 mm cavity. After perforation, the area where #40 K type file appeared was recorded as the actual length (AL) and the length measured by EAL devices both in dry canals and in the presence of solutions was recorded as the electronic measurement (EL). All electronic measurements were statistically compared with the actual length. Results: All EALs achieved AL-consistent results on EL measures. Consistency was determined using Root ZX Mini measurements in dry canals and canals irrigated with saline. The consistency of EL and AL conducted with Raypex 6 and Apex ID in canals irrigated with NaOCl, saline, and EDTA was found. There was a statistically significant difference in ELs with irrigation solutions among all EALs (p<0.05). In the dry environment, there was no statistically significant difference between the EALs (p>0.05). A consistency was discovered between ELs generated with the Root ZX Mini and ALs in both dry and saline-irrigated canals. Consistency was observed between ELs measured with Raypex 6 and Apex ID and ALs in canals irrigated with NaOCl, saline, and EDTA. Conclusion: The accuracy rates of the EALs used in this research were 97%–100% in the 1 mm range and 83%–92% in the 0.5 mm range. Despite the fact that ALs and ELs differed statistically significantly at the 0.05 level, these variations weren't thought to be clinically relevant. In the presence of conditions with different electro conductors, EALs from different generations may be used safely, and in a range of canal situations, these devices can yield measurements that are most similar to the AL.
目的:电子根尖定位仪(EAL)研究最多的课题之一是根管状况对 EAL 精确度的影响。本研究评估了 Root ZX Mini、Raypex 6 和 Apex ID 在干燥环境和生理盐水、乙二胺四乙酸 (EDTA) 和次氯酸钠 (NaOCl) 溶液中检测根穿孔的准确性:研究选取了 64 颗人类上颌第一磨牙的中颊根。使用 1 号 Freze Beutherlock Peeso 从颊面根表面对这些根管进行穿孔,形成 0.4 毫米的空腔。穿孔后,40 号 K 型锉出现的区域被记录为实际长度 (AL),而在干燥根管和溶液存在的情况下通过 EAL 设备测量的长度被记录为电子测量值 (EL)。所有电子测量值都与实际长度进行了统计比较:结果:所有 EAL 在 EL 测量上都达到了与 AL 一致的结果。使用 Root ZX Mini 对干燥的根管和生理盐水灌溉的根管进行测量,可以确定测量结果的一致性。使用 Raypex 6 和 Apex ID 在用 NaOCl、生理盐水和 EDTA 灌溉的根管中进行的 EL 和 AL 测量结果一致。在所有 EAL 中,使用灌溉溶液的 EL 有显著的统计学差异(P0.05)。使用 Root ZX Mini 生成的 ELs 与干燥和盐水灌溉下的 ALs 之间具有一致性。用 Raypex 6 和 Apex ID 测量的 EL 与用 NaOCl、生理盐水和 EDTA 灌溉的牙管中的 AL 之间也有一致性:本研究中使用的 EAL 在 1 毫米范围内的准确率为 97%-100%,在 0.5 毫米范围内的准确率为 83%-92%。尽管 AL 和 EL 在 0.05 的统计学水平上存在显著差异,但这些差异被认为与临床无关。在存在不同电导体的条件下,可以安全地使用不同世代的EAL,而且在一系列的牙槽骨情况下,这些设备可以获得与AL最相似的测量结果。
{"title":"The Accuracy of Different Apex Locator Systems in Detecting Root Perforations in the Presence of Different Irrigation Solutions","authors":"Oğuz Burhan Çetinkaya, Emre Çulha, Uğur Aydın","doi":"10.58600/eurjther1936","DOIUrl":"https://doi.org/10.58600/eurjther1936","url":null,"abstract":"Objective: One of the most studied topics in electronic apex locators (EALs) is the effect of root canal condition on the accuracy of EALs. In this study, the accuracy of Root ZX Mini, Raypex 6, and Apex ID in detecting root perforation was evaluated in a dry environment and in the presence of saline, ethylenediamine tetraacetic acid (EDTA), and sodium hypochlorite (NaOCl) solutions.\u0000Methods: The mesiobuccal roots of 64 human maxillary first molars were selected for the study. These root canals were perforated from buccal root surface using a #1 Freze Beutherlock Peeso to form a 0.4 mm cavity. After perforation, the area where #40 K type file appeared was recorded as the actual length (AL) and the length measured by EAL devices both in dry canals and in the presence of solutions was recorded as the electronic measurement (EL). All electronic measurements were statistically compared with the actual length.\u0000Results: All EALs achieved AL-consistent results on EL measures. Consistency was determined using Root ZX Mini measurements in dry canals and canals irrigated with saline. The consistency of EL and AL conducted with Raypex 6 and Apex ID in canals irrigated with NaOCl, saline, and EDTA was found. There was a statistically significant difference in ELs with irrigation solutions among all EALs (p<0.05). In the dry environment, there was no statistically significant difference between the EALs (p>0.05). A consistency was discovered between ELs generated with the Root ZX Mini and ALs in both dry and saline-irrigated canals. Consistency was observed between ELs measured with Raypex 6 and Apex ID and ALs in canals irrigated with NaOCl, saline, and EDTA.\u0000Conclusion: The accuracy rates of the EALs used in this research were 97%–100% in the 1 mm range and 83%–92% in the 0.5 mm range. Despite the fact that ALs and ELs differed statistically significantly at the 0.05 level, these variations weren't thought to be clinically relevant. In the presence of conditions with different electro conductors, EALs from different generations may be used safely, and in a range of canal situations, these devices can yield measurements that are most similar to the AL.","PeriodicalId":42642,"journal":{"name":"European Journal of Therapeutics","volume":"6 11","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138966534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Grillo, Alexandre Meireles Borba, Y. Slusarenko da Silva, Mariana Aparecida Brozoski Aparecida Brozoski
Objectives: The amount of dermal filler procedures is increasing. Some patients opt to undergo dermal fillers instead of orthognathic surgery to treat unesthetic complaints from dentofacial deformities. The aim of this work is to carry out a literature review with regard to a comparison of role and limitations between aesthetic indications of orthognathic surgery and dermal fillers. Methods: A scoping review was performed according to the PRISMA-ScR guidelines on Pubmed, Web of Science and Google Scholar. A second search was conducted to highlight topics very close subjects to the main subject: the importance of social media and measures to avoid litigation in facial aesthetics. Results: Literature on the subject is very rare. Clinical facial analysis is fundamental to both procedures. Although patients wishes are important issues, facial analysis cannot be neglected due to its objectivity. Body dysmorphic disorder is considered a contraindication for both procedures. Social media is important in patient decision-making, but should not influence treatment planning by experts. Preventing litigation or reducing financial and reputational damage can be accomplished with a few simple steps. Conclusions: A helpful list of indications and particularly contraindications for orthognathic surgery and dermal fillers was drawn up. Dermal fillers must not substitute orthognathic surgery. Further studies are urgently needed to discuss this contemporary issue.
目的:皮肤填充手术的数量正在不断增加。一些患者选择接受皮肤填充术而不是正颌外科手术,以治疗颌面部畸形引起的不美观症状。这项工作的目的是就正颌外科手术和皮肤填充术在美学适应症方面的作用和局限性的比较进行文献综述:方法:根据PRISMA-ScR指南在Pubmed、Web of Science和Google Scholar上进行了范围审查。第二项搜索是为了突出与主题非常接近的主题:社交媒体的重要性和避免面部美容诉讼的措施:有关该主题的文献非常罕见。临床面部分析是两种手术的基础。尽管患者的意愿是重要问题,但面部分析的客观性不容忽视。身体畸形障碍被认为是两种手术的禁忌症。社交媒体对患者的决策很重要,但不应影响专家的治疗计划。通过几个简单的步骤就可以防止诉讼或减少经济和名誉损失:本文列出了正颌外科手术和皮肤填充剂的适应症,尤其是禁忌症。皮肤填充剂不能替代正颌外科手术。我们亟需开展更多的研究来讨论这一当代问题。
{"title":"A Scoping Review of the Role and Limitations of Surgical Versus Non-Surgical Management of Dentofacial Deformities","authors":"R. Grillo, Alexandre Meireles Borba, Y. Slusarenko da Silva, Mariana Aparecida Brozoski Aparecida Brozoski","doi":"10.58600/eurjther1933","DOIUrl":"https://doi.org/10.58600/eurjther1933","url":null,"abstract":"Objectives: The amount of dermal filler procedures is increasing. Some patients opt to undergo dermal fillers instead of orthognathic surgery to treat unesthetic complaints from dentofacial deformities. The aim of this work is to carry out a literature review with regard to a comparison of role and limitations between aesthetic indications of orthognathic surgery and dermal fillers.\u0000Methods: A scoping review was performed according to the PRISMA-ScR guidelines on Pubmed, Web of Science and Google Scholar. A second search was conducted to highlight topics very close subjects to the main subject: the importance of social media and measures to avoid litigation in facial aesthetics.\u0000Results: Literature on the subject is very rare. Clinical facial analysis is fundamental to both procedures. Although patients wishes are important issues, facial analysis cannot be neglected due to its objectivity. Body dysmorphic disorder is considered a contraindication for both procedures. Social media is important in patient decision-making, but should not influence treatment planning by experts. Preventing litigation or reducing financial and reputational damage can be accomplished with a few simple steps.\u0000Conclusions: A helpful list of indications and particularly contraindications for orthognathic surgery and dermal fillers was drawn up. Dermal fillers must not substitute orthognathic surgery. Further studies are urgently needed to discuss this contemporary issue.","PeriodicalId":42642,"journal":{"name":"European Journal of Therapeutics","volume":"14 7","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138966322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Diabetes Mellitus (DM) not only causes hyperglycemia but also leads to clinical challenges involving respiratory functional impairments. The contraction of the diaphragm reduces pleural pressure, thereby contributing significantly to the process of breathing. This study examines the functional impairments in diaphragm muscle isometric contraction parameters due to increased reactive oxygen species (ROS) associated with DM, as well as the effects of MitoTEMPO, a mitochondria-specific antioxidant, on these impairments. Methods: Wistar Albino male rats at 12-14 weeks of age were randomly divided into three groups: the control group (CON, n=6), the diabetes group (DM, n=6), and the diabetes + MitoTEMPO (MT, n=6) group. A single dose of 50 mg/kg streptozotocin (STZ) was administered to the rats in the DM and MT groups. When the rats in the MT group reached a blood glucose level of 300 mg/dl, they were administered MitoTEMPO at a dose of 0.7 mg/kg/day for 28 days. Isometric contraction recordings were obtained from diaphragm muscle preparations isolated from the experimental animals at the end of the 28-day period. Results: Although the effectiveness of mitochondria-specific antioxidants in reducing blood glucose levels in DM is debated in the literature, results for the MT group were interestingly indicative of a statistically significant decrease in blood glucose levels following MitoTEMPO administration at the end of the fourth week. Furthermore, MitoTEMPO exhibited therapeutic effects on diaphragm muscle contraction parameters impaired by DM. Conclusion: The findings suggest that in DM patients, MitoTEMPO could be utilized for blood glucose control and might also be effective in the treatment of DM-induced diaphragm muscle mechanical dysfunction.
{"title":"The Effect of MitoTEMPO on Rat Diaphragm Muscle Contraction Parameters in an Experimental Diabetes Model Induced with Streptozotocin","authors":"Ahmet Akkoca, S. Tuncer, M. Çelen, N. Dalkılıç","doi":"10.58600/eurjther1912","DOIUrl":"https://doi.org/10.58600/eurjther1912","url":null,"abstract":"Objective: Diabetes Mellitus (DM) not only causes hyperglycemia but also leads to clinical challenges involving respiratory functional impairments. The contraction of the diaphragm reduces pleural pressure, thereby contributing significantly to the process of breathing. This study examines the functional impairments in diaphragm muscle isometric contraction parameters due to increased reactive oxygen species (ROS) associated with DM, as well as the effects of MitoTEMPO, a mitochondria-specific antioxidant, on these impairments.\u0000Methods: Wistar Albino male rats at 12-14 weeks of age were randomly divided into three groups: the control group (CON, n=6), the diabetes group (DM, n=6), and the diabetes + MitoTEMPO (MT, n=6) group. A single dose of 50 mg/kg streptozotocin (STZ) was administered to the rats in the DM and MT groups. When the rats in the MT group reached a blood glucose level of 300 mg/dl, they were administered MitoTEMPO at a dose of 0.7 mg/kg/day for 28 days. Isometric contraction recordings were obtained from diaphragm muscle preparations isolated from the experimental animals at the end of the 28-day period.\u0000Results: Although the effectiveness of mitochondria-specific antioxidants in reducing blood glucose levels in DM is debated in the literature, results for the MT group were interestingly indicative of a statistically significant decrease in blood glucose levels following MitoTEMPO administration at the end of the fourth week. Furthermore, MitoTEMPO exhibited therapeutic effects on diaphragm muscle contraction parameters impaired by DM.\u0000Conclusion: The findings suggest that in DM patients, MitoTEMPO could be utilized for blood glucose control and might also be effective in the treatment of DM-induced diaphragm muscle mechanical dysfunction.","PeriodicalId":42642,"journal":{"name":"European Journal of Therapeutics","volume":"485 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138982980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The increasing use of electronic devices, accompanied by advancing technologies, has led to heightened exposure to non-ionizing electromagnetic radiation (EMR). This exposure instigates the accumulation of free radicals and oxidative damage in tissues, consequently impacting biological systems. Notably, the testis is among the tissues adversely affected by EMR. Numerous studies have highlighted the pivotal role of the testis in sperm production, emphasizing the potential implications of any damage on the reproductive system. This study aims to assess the levels of lipid peroxidation through histological evaluation in the testicular tissue of prepubertal male rats exposed to electromagnetic radiation at varying electric field intensities within the 2.45 GHz radiofrequency (RF) range. Methods: The experimental group comprises six subdivisions, including a sham control group, as well as groups exposed to varying electric field strengths (EFS) of 0.6 V/m, 1.9 V/m, 5 V/m, 10 V/m, and 15 V/m, respectively. Excluding the sham control group, the remaining subgroups were subjected to a daily 2.45 GHz RF exposure for 1 hour starting immediately after fertilization. This exposure to different electric field intensities continued for 45 days post-birth. Results: The samples obtained from the RF radiation-exposed rats exhibited elevated malondialdehyde (MDA) values and decreased glutathione (GSH) values in the testicular tissue. Furthermore, a comparative analysis between the microwave radiation-exposed group and the control group revealed distinct histological alterations in the testicular tissue. Conclusion: In conclusion, our findings indicate that exposure to microwave radiation at an electric field intensity of 15 V/m can lead to significant histopathological and oxidative parameter changes in Wistar rats. These results underscore the potential effects of such exposure on human health.
{"title":"Effects of Exposure to Radiofrequency at 2.45 GHz on Structural Changes Associated with Lipid Peroxidation in Prepubertal Rat Testicular Tissue","authors":"Aysegul Karadayi, Nilufer Akgun Unal, Elif Gulbahce Mutlu, Begum Korunur Engiz, Ahmet Akkoca, Salih Varol","doi":"10.58600/eurjther1875","DOIUrl":"https://doi.org/10.58600/eurjther1875","url":null,"abstract":"Objective: The increasing use of electronic devices, accompanied by advancing technologies, has led to heightened exposure to non-ionizing electromagnetic radiation (EMR). This exposure instigates the accumulation of free radicals and oxidative damage in tissues, consequently impacting biological systems. Notably, the testis is among the tissues adversely affected by EMR. Numerous studies have highlighted the pivotal role of the testis in sperm production, emphasizing the potential implications of any damage on the reproductive system. This study aims to assess the levels of lipid peroxidation through histological evaluation in the testicular tissue of prepubertal male rats exposed to electromagnetic radiation at varying electric field intensities within the 2.45 GHz radiofrequency (RF) range.\u0000Methods: The experimental group comprises six subdivisions, including a sham control group, as well as groups exposed to varying electric field strengths (EFS) of 0.6 V/m, 1.9 V/m, 5 V/m, 10 V/m, and 15 V/m, respectively. Excluding the sham control group, the remaining subgroups were subjected to a daily 2.45 GHz RF exposure for 1 hour starting immediately after fertilization. This exposure to different electric field intensities continued for 45 days post-birth.\u0000Results: The samples obtained from the RF radiation-exposed rats exhibited elevated malondialdehyde (MDA) values and decreased glutathione (GSH) values in the testicular tissue. Furthermore, a comparative analysis between the microwave radiation-exposed group and the control group revealed distinct histological alterations in the testicular tissue.\u0000Conclusion: In conclusion, our findings indicate that exposure to microwave radiation at an electric field intensity of 15 V/m can lead to significant histopathological and oxidative parameter changes in Wistar rats. These results underscore the potential effects of such exposure on human health.","PeriodicalId":42642,"journal":{"name":"European Journal of Therapeutics","volume":"39 8","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138596239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objectives: The aim of this study is to evaluate and compare the bond strength of different dentin desensitizers and self-adhesive resin cements to dentin surfaces. Methods: The flat dentin surfaces of 72 wisdom molar teeth were randomly divided into six groups for bond strength analysis (n=12): Group CP: No desensitizer + Primer II A&B+ Panavia F 2.0, Group CM: No desensitizer+ Primer A&B+ Multilink N, Group TP: Tokuyama Shield Force+ Primer II A&B + Panavia F 2.0, Group TM: Tokuyama Shield Force + Primer A&B+ Multilink N, Group UP: Universal dentin sealant + Primer II A&B+ Panavia F 2.0 and Group UM: Universal dentin sealent + Primer A&B+ Multilink N. The shear bond strength test was performed using a universal testing machine (0.5 mm/min). ANOVA test was used to detect significant differences at a p < 0.05. Results: The results indicated that bond strength values varied according to the desensitizing and resin cement materials (p < 0.05). The Tokuyama Shield Force desensitizer did not affect the bond strength of the resin cements to dentin (p > 0.05). Conclusion: The different types of dentin desensitizer applications affected on the shear bond strength results of the self-adhesive resin cements.
目的:本研究的目的是评估和比较不同的牙本质脱敏剂和自粘树脂水泥与牙本质表面的结合强度。方法:将72颗智慧磨牙牙本质平面随机分为6组(n=12)进行结合强度分析:CP组:无脱敏剂+ Primer II A&B+ Panavia F 2.0, CM组:无脱敏剂+ Primer A&B+ Multilink n, TP组:Tokuyama Shield Force+ Primer II A&B+ Panavia F 2.0, TM组:Tokuyama Shield Force+ Primer A&B+ Multilink n, UP组:通用牙本质密封剂+ Primer II A&B+ Panavia F 2.0, UM组:万能牙本质密封剂+底漆A&B+ Multilink N.剪切粘结强度试验采用万能试验机(0.5 mm/min)。采用方差分析(ANOVA)检验,p < 0.05。结果:黏结强度值因脱敏材料和树脂水泥材料的不同而不同(p < 0.05)。Tokuyama盾构力脱敏剂对牙本质黏结强度无显著影响(p > 0.05)。结论:不同类型牙本质脱敏剂的应用对自粘树脂胶结物的剪切粘结强度有影响。
{"title":"The Effect of Different Dentin Desensitizers and Self- Adhesive Resin Cement on Shear Bond Strength: In Vitro Study","authors":"Işıl Keçik Büyükhatipoğlu, Derya Gürsel Sürmelioğlu","doi":"10.58600/eurjther1892","DOIUrl":"https://doi.org/10.58600/eurjther1892","url":null,"abstract":"Objectives: The aim of this study is to evaluate and compare the bond strength of different dentin desensitizers and self-adhesive resin cements to dentin surfaces.\u0000Methods: The flat dentin surfaces of 72 wisdom molar teeth were randomly divided into six groups for bond strength analysis (n=12): Group CP: No desensitizer + Primer II A&B+ Panavia F 2.0, Group CM: No desensitizer+ Primer A&B+ Multilink N, Group TP: Tokuyama Shield Force+ Primer II A&B + Panavia F 2.0, Group TM: Tokuyama Shield Force + Primer A&B+ Multilink N, Group UP: Universal dentin sealant + Primer II A&B+ Panavia F 2.0 and Group UM: Universal dentin sealent + Primer A&B+ Multilink N. The shear bond strength test was performed using a universal testing machine (0.5 mm/min). ANOVA test was used to detect significant differences at a p < 0.05.\u0000Results: The results indicated that bond strength values varied according to the desensitizing and resin cement materials (p < 0.05). The Tokuyama Shield Force desensitizer did not affect the bond strength of the resin cements to dentin (p > 0.05).\u0000Conclusion: The different types of dentin desensitizer applications affected on the shear bond strength results of the self-adhesive resin cements.","PeriodicalId":42642,"journal":{"name":"European Journal of Therapeutics","volume":"43 9","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138596314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mehmet Kemal Tümer, Adem Keskin, Recai Acı, S. Yıgıt
Objective: Odontogenic cysts that cause bone destruction can exhibit various types of metaplasia. Inherited genetic variants in codons 72 and 175, the hotspot codons of p53, known as the guardian of the genome, can cause a wide variety of cancers. We aimed to investigate the effects of the p53 codon 72 and p53 codon 175 variants on odontogenic cyst formation. Methods: This research encompassed 71 individuals with odontogenic cysts and 90 without any conditions as a control group. After DNA was extracting, the p53 codon 72 was detected using PCR techniques, while p53 codon 175 was identified through allele-specific amplification-PCR. Results: The presence of the p53 codon 72 GG genotype and its G allele was less frequent in the group with odontogenic cysts compared to the healthy participants. Conversely, the C allele was found more often in the cyst-afflicted group. For the p53 codon 175, the AA genotype and A allele were more common in the affected group, while the G allele was more predominant in the control group. Conclusion: The p53 codon 175 AA genotype and A allele, p53 codon 72 C allele, and p53 codon 72/codon 175 CCAA combined genotype may be associated with odontagenic cyst formation. Individuals with this allele and genotype can be considered at risk for odontagenic cyst formation.
{"title":"The Relationship Between Odontogenic Cyst and P53 Codon 72 And P53 Codon 175 Variants in Turkish Patients","authors":"Mehmet Kemal Tümer, Adem Keskin, Recai Acı, S. Yıgıt","doi":"10.58600/eurjther1911","DOIUrl":"https://doi.org/10.58600/eurjther1911","url":null,"abstract":"Objective: Odontogenic cysts that cause bone destruction can exhibit various types of metaplasia. Inherited genetic variants in codons 72 and 175, the hotspot codons of p53, known as the guardian of the genome, can cause a wide variety of cancers. We aimed to investigate the effects of the p53 codon 72 and p53 codon 175 variants on odontogenic cyst formation.\u0000Methods: This research encompassed 71 individuals with odontogenic cysts and 90 without any conditions as a control group. After DNA was extracting, the p53 codon 72 was detected using PCR techniques, while p53 codon 175 was identified through allele-specific amplification-PCR.\u0000Results: The presence of the p53 codon 72 GG genotype and its G allele was less frequent in the group with odontogenic cysts compared to the healthy participants. Conversely, the C allele was found more often in the cyst-afflicted group. For the p53 codon 175, the AA genotype and A allele were more common in the affected group, while the G allele was more predominant in the control group.\u0000Conclusion: The p53 codon 175 AA genotype and A allele, p53 codon 72 C allele, and p53 codon 72/codon 175 CCAA combined genotype may be associated with odontagenic cyst formation. Individuals with this allele and genotype can be considered at risk for odontagenic cyst formation.","PeriodicalId":42642,"journal":{"name":"European Journal of Therapeutics","volume":"93 7","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138595870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}