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Laccase-mimicking Cu@CDs-enabled bioinspired immunosensor using CuO nanoparticles for voltametric detection of serum thyroxine level in patients with hyperthyroidism or hypothyroidism

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-01-28 DOI: 10.1016/j.snr.2025.100291

Mohammad Ali Farzin, Seyed Morteza Naghib

In this study, for the first time, a voltammetric bioinspired immunosensor was fabricated to determine free thyroxine (T4) based on the blocking nanozyme-dependent signaling pathway through masking the active sites of the laccase-mimicking Cu-doped CDs (Cu@CDs). The focus shifted to monitoring a vital biomarker for thyroid diseases using a metal-containing nanozyme, showcasing high potential to catalyze the oxidation of phenolic compounds. Thanks to the active sites of Cu@CD nanozyme, the electrocatalytic oxidation of hydroquinone (HQ), as a signal probe, was performed at a high rate. The CuO nanostructures also increased the conductivity as well as specific surface area of electrode. Despite signal amplification of HQ, the binding of antibody to carbon dot reduced enzymatic activity of nanozyme and subsequently resulted in a considerable decrease in the current intensity of GCE/CuO nanoparticles/Cu@CDs-HQ. The bioconjugation of T4 with anti-T4 antibody resulted in the further coverage of the active sites of nanozyme. Therefore, the current of GCE/CuO nanoparticles/Cu@CDs-HQ/Ab decreased proportionally to the T4 concentration in a DLR (dynamic linear range) of 10.0 pM-8.0 nM which comprises the reference range for free T4 (11.5–22.7 pM). The LOD (limit of detection) of 2.8 pM, was lower compared to the values reported in previous studies. Furthermore, this study exhibited the key finding that the highly specific interaction of T4 with immobilized antibody led to a remarkable diminish in the immunosensor signal compared to the interferers. More importantly, spike-recovery studies demonstrated the high potential of the designed immunosensor to detect serum T4 levels in patients with hyperthyroidism or hypothyroidism.

{"title":"Laccase-mimicking Cu@CDs-enabled bioinspired immunosensor using CuO nanoparticles for voltametric detection of serum thyroxine level in patients with hyperthyroidism or hypothyroidism","authors":"Mohammad Ali Farzin, Seyed Morteza Naghib","doi":"10.1016/j.snr.2025.100291","DOIUrl":"10.1016/j.snr.2025.100291","url":null,"abstract":"<div><div>In this study, for the first time, a voltammetric bioinspired immunosensor was fabricated to determine free thyroxine (T4) based on the blocking nanozyme-dependent signaling pathway through masking the active sites of the laccase-mimicking Cu-doped CDs (Cu@CDs). The focus shifted to monitoring a vital biomarker for thyroid diseases using a metal-containing nanozyme, showcasing high potential to catalyze the oxidation of phenolic compounds. Thanks to the active sites of Cu@CD nanozyme, the electrocatalytic oxidation of hydroquinone (HQ), as a signal probe, was performed at a high rate. The CuO nanostructures also increased the conductivity as well as specific surface area of electrode. Despite signal amplification of HQ, the binding of antibody to carbon dot reduced enzymatic activity of nanozyme and subsequently resulted in a considerable decrease in the current intensity of GCE/CuO nanoparticles/Cu@CDs-HQ. The bioconjugation of T4 with anti-T4 antibody resulted in the further coverage of the active sites of nanozyme. Therefore, the current of GCE/CuO nanoparticles/Cu@CDs-HQ/Ab decreased proportionally to the T4 concentration in a DLR (dynamic linear range) of 10.0 pM-8.0 nM which comprises the reference range for free T4 (11.5–22.7 pM). The LOD (limit of detection) of 2.8 pM, was lower compared to the values reported in previous studies. Furthermore, this study exhibited the key finding that the highly specific interaction of T4 with immobilized antibody led to a remarkable diminish in the immunosensor signal compared to the interferers. More importantly, spike-recovery studies demonstrated the high potential of the designed immunosensor to detect serum T4 levels in patients with hyperthyroidism or hypothyroidism.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100291"},"PeriodicalIF":6.5,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface reaction mechanism and characteristics of 2-dimensional TiO2 and 0-dimensional Ag nanocomposites specialized for H2S gas sensing at room temperature

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-01-28 DOI: 10.1016/j.snr.2025.100290

Un Yup Lee , Min Young Kim , Kyu Hyoung Lee , Sol Han , Seung Yong Lee , Ali Mirzaei , Sun-Woo Choi , Myung Sik Choi , Changhyun Jin , Jeong Yun Hwang

In this study, we provide the synthesis of pristine TiO₂ nanosheets (NSs) via a simple flux method and their decoration with Ag nanoparticles (NPs) using a hydrothermal method for room-temperature H₂S gas sensing. Gas sensing experiments were conducted at 25 °C, evaluating the response of the Ag NPs-decorated TiO₂ NSs to various gases. Results demonstrated the gas sensor exhibited a superior response to H₂S gas compared to other gases, outperforming the pristine TiO₂ NSs gas sensor. Additionally, its response to H₂S gas surpassed that of the TiO₂ NSs gas sensor, highlighting the promising role of Ag decoration for H₂S gas detection. Sensing behavior in humid atmosphere was also investigated. The improved H₂S gas sensing performance can be attributed to the catalytic effect of Ag, the formation of Ag/TiO₂ heterojunctions, and the large surface area of the TiO₂ NSs. This study introduces a straightforward approach to enhance the gas sensing capabilities of metal oxide NSs.

{"title":"Surface reaction mechanism and characteristics of 2-dimensional TiO2 and 0-dimensional Ag nanocomposites specialized for H2S gas sensing at room temperature","authors":"Un Yup Lee , Min Young Kim , Kyu Hyoung Lee , Sol Han , Seung Yong Lee , Ali Mirzaei , Sun-Woo Choi , Myung Sik Choi , Changhyun Jin , Jeong Yun Hwang","doi":"10.1016/j.snr.2025.100290","DOIUrl":"10.1016/j.snr.2025.100290","url":null,"abstract":"<div><div>In this study, we provide the synthesis of pristine TiO<sub>2</sub> nanosheets (NSs) via a simple flux method and their decoration with Ag nanoparticles (NPs) using a hydrothermal method for room-temperature H<sub>2</sub>S gas sensing. Gas sensing experiments were conducted at 25 °C, evaluating the response of the Ag NPs-decorated TiO<sub>2</sub> NSs to various gases. Results demonstrated the gas sensor exhibited a superior response to H<sub>2</sub>S gas compared to other gases, outperforming the pristine TiO<sub>2</sub> NSs gas sensor. Additionally, its response to H<sub>2</sub>S gas surpassed that of the TiO<sub>2</sub> NSs gas sensor, highlighting the promising role of Ag decoration for H<sub>2</sub>S gas detection. Sensing behavior in humid atmosphere was also investigated. The improved H<sub>2</sub>S gas sensing performance can be attributed to the catalytic effect of Ag, the formation of Ag/TiO<sub>2</sub> heterojunctions, and the large surface area of the TiO<sub>2</sub> NSs. This study introduces a straightforward approach to enhance the gas sensing capabilities of metal oxide NSs.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100290"},"PeriodicalIF":6.5,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143334064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology controlled fabrication of zinc phosphate hierarchical microspheres for room temperature ammonia gas sensor

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-01-27 DOI: 10.1016/j.snr.2025.100288

Khalida Akhtar , Naila Zubair , Neelam Zeb , Syed Sajjad Ali Shah , Zia Ullah Khan , Hina Khalid

Hierarchical Zinc phosphate nano/microstructures with controlled morphological features were synthesized in aqueous solutions through simple and economical route without using any type of surfactant or template. SEM analysis revealed novel morphologies of the synthesized powders i.e., hierarchical microspheres, urchin like microspheres, nanoflowers and nanoellipsoids and unfolded that produced morphologies strongly depended upon the applied experimental conditions. Selected batches of the synthesized powders i.e., hierarchical microspheres, urchin like microspheres and hierarchical nanoellipsoids were selected for further analysis using XRD, FT-IR, BET analysis and gas sensing application and termed as Zinc Phosphate 1 (ZP1), Zinc Phosphate 2 (ZP2), and Zinc Phosphate 3 (ZP3), respectively. XRD results confirmed the crystalline nature of the as-prepared powders with crystallite sizes 16.5 nm, 30. 15 nm and 37.01 nm, for ZP1, ZP2 and ZP3, respectively. Evaluation of gas sensing properties of the selected samples revealed that sensors based on ZP1 showed highest gas sensing response of 89 % with response recovery time 31/12 s, towards 5 ppm ammonia at room temperature. In addition, the lowest detection limit was investigated to be <1 ppm. Because of the excellent gas sensing performance, the synthesized zinc phosphate structures could be employed as promising candidates for developing highly sensitive and selective room temperature ammonia gas sensor.

{"title":"Morphology controlled fabrication of zinc phosphate hierarchical microspheres for room temperature ammonia gas sensor","authors":"Khalida Akhtar , Naila Zubair , Neelam Zeb , Syed Sajjad Ali Shah , Zia Ullah Khan , Hina Khalid","doi":"10.1016/j.snr.2025.100288","DOIUrl":"10.1016/j.snr.2025.100288","url":null,"abstract":"<div><div>Hierarchical Zinc phosphate nano/microstructures with controlled morphological features were synthesized in aqueous solutions through simple and economical route without using any type of surfactant or template. SEM analysis revealed novel morphologies of the synthesized powders i.e., hierarchical microspheres, urchin like microspheres, nanoflowers and nanoellipsoids and unfolded that produced morphologies strongly depended upon the applied experimental conditions. Selected batches of the synthesized powders i.e., hierarchical microspheres, urchin like microspheres and hierarchical nanoellipsoids were selected for further analysis using XRD, FT-IR, BET analysis and gas sensing application and termed as Zinc Phosphate 1 (ZP1), Zinc Phosphate 2 (ZP2), and Zinc Phosphate 3 (ZP3), respectively. XRD results confirmed the crystalline nature of the as-prepared powders with crystallite sizes 16.5 nm, 30. 15 nm and 37.01 nm, for ZP1, ZP2 and ZP3, respectively. Evaluation of gas sensing properties of the selected samples revealed that sensors based on ZP1 showed highest gas sensing response of 89 % with response recovery time 31/12 s, towards 5 ppm ammonia at room temperature. In addition, the lowest detection limit was investigated to be <1 ppm. Because of the excellent gas sensing performance, the synthesized zinc phosphate structures could be employed as promising candidates for developing highly sensitive and selective room temperature ammonia gas sensor.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100288"},"PeriodicalIF":6.5,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A tactile sensor for recognition of softness using interlocking structure of carbon nanoparticle- polydimethylsiloxane composite

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-01-24 DOI: 10.1016/j.snr.2025.100289

Sangmin Lee, Jaewon Jang, Wanjun Park

This study introduces a novel tactile sensor designed with an interlocking structure of polydimethylsiloxane (PDMS) embedded with carbon nanoparticles (CNP), aimed at enhancing the detection and differentiation of material softness. This sensor emulates the human tactile sense by detecting softness through measuring not only the pressure applied but also the transient state during material deformation to reach the indentation depth, thus providing a comprehensive softness profile. Our results reveal that the sensor effectively recognizes softness across a variety of materials and working environments, considering tolerances against operational variations. This is supported by tests conducted using a one-dimensional convolutional neural network (1D-CNN).

引用次数: 0

A highly sensitive tapered fiber biosensor modified by PDMS combustion product and graphene oxide for MUC1 detection

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-01-23 DOI: 10.1016/j.snr.2025.100287

Bo Wang, Shanren Liu, Mengmeng Gao, Yuanjun Li, Heer Yang, Cai Sun, Qi Guo, Yongsen Yu

It is a critical challenge to develop reliable, stable, and sensitive biosensors for specific detection of mucin 1 (MUC1). In this paper, a label-free biosensor based on polydimethylsiloxane (PDMS) combustion product and graphene oxide (GO) functionalized tapered fiber (PGTF) was proposed and demonstrated for high sensitivity MUC1 detection. The PDMS combustion product is mainly composed of SiO₂, which can be firmly bonded to the surface of the fiber, effectively increasing the specific surface area of the fiber. At the same time, the combination of the PDMS combustion product and the fiber cladding material will generate defects in the cladding, resulting in easier leakage of light inside the fiber core. Therefore, a strong evanescent field and interference effect can be obtained without drawing the tapered fiber waist very thin, which contributes to improving the sensitivity of fiber biosensor. In addition, GO has good biological affinity, a large specific surface area, and abundant functional groups, which can provide more binding sites for the attachment of biomolecule. The detection limit of the PGTF is 0.11 pM when the diameter of the fiber taper is 50 μm and modified with five layers of PDMS combustion product. Experimentally demonstrated sensor specificity and reproducibility, indicating that the sensor has a practical value for real applications. The PGTF has great potential as a simple, sensitive, and label-free assay in the field of cancer marker detection.

{"title":"A highly sensitive tapered fiber biosensor modified by PDMS combustion product and graphene oxide for MUC1 detection","authors":"Bo Wang, Shanren Liu, Mengmeng Gao, Yuanjun Li, Heer Yang, Cai Sun, Qi Guo, Yongsen Yu","doi":"10.1016/j.snr.2025.100287","DOIUrl":"10.1016/j.snr.2025.100287","url":null,"abstract":"<div><div>It is a critical challenge to develop reliable, stable, and sensitive biosensors for specific detection of mucin 1 (MUC1). In this paper, a label-free biosensor based on polydimethylsiloxane (PDMS) combustion product and graphene oxide (GO) functionalized tapered fiber (PGTF) was proposed and demonstrated for high sensitivity MUC1 detection. The PDMS combustion product is mainly composed of SiO<sub>2</sub>, which can be firmly bonded to the surface of the fiber, effectively increasing the specific surface area of the fiber. At the same time, the combination of the PDMS combustion product and the fiber cladding material will generate defects in the cladding, resulting in easier leakage of light inside the fiber core. Therefore, a strong evanescent field and interference effect can be obtained without drawing the tapered fiber waist very thin, which contributes to improving the sensitivity of fiber biosensor. In addition, GO has good biological affinity, a large specific surface area, and abundant functional groups, which can provide more binding sites for the attachment of biomolecule. The detection limit of the PGTF is 0.11 pM when the diameter of the fiber taper is 50 μm and modified with five layers of PDMS combustion product. Experimentally demonstrated sensor specificity and reproducibility, indicating that the sensor has a practical value for real applications. The PGTF has great potential as a simple, sensitive, and label-free assay in the field of cancer marker detection.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100287"},"PeriodicalIF":6.5,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging EM wave sensors for non-invasive glucose monitoring: Review, techniques and developments

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-01-18 DOI: 10.1016/j.snr.2025.100282

Sudershan Dutt , Rajat Thakur , Abhishek Kandwal , Pawan Kumar , Rohit Jasrotia , Natrayan Lakshmaiya , Louis WY Liu , Sachin Kumar , Bancha Luadang , Choon Kit Chan

As a chronic disease, diabetes has impacted millions of individuals across the globe and is spreading at a very fast rate among adults mainly. Continuous observations of essential indicators like pulse rate, arterial pressure, and blood sugar levels, and oxygen saturation are crucial to maintain a healthy life and preventing severe complications associated with these. Using non-invasive method of blood glucose monitoring has paved its way to be one of the most prominent safest technique without harming the skin of the patients. This examination delves into the application of planar resonant sensors utilizing microwaves for quantifying glucose concentrations. The sensors based on RF EM waves mainly operating in the region from few MHz to GHz frequencies have garnered notable attention in the scientific community in recent years due to their significant contributions. They can detect alterations in dielectric properties caused by fluctuations in glucose concentrations. These sensors exhibit electrical reactions that rely on the dielectric properties of their surroundings. This review offers a current assessment of this sensing methodology, categorized by sensing parameters, and suggests typical non-invasive microwave sensor varieties for assessment. It further examines the key aspects of different sensor types, outlines potential future avenues, and addresses challenges in the field of glucose monitoring.

{"title":"Emerging EM wave sensors for non-invasive glucose monitoring: Review, techniques and developments","authors":"Sudershan Dutt , Rajat Thakur , Abhishek Kandwal , Pawan Kumar , Rohit Jasrotia , Natrayan Lakshmaiya , Louis WY Liu , Sachin Kumar , Bancha Luadang , Choon Kit Chan","doi":"10.1016/j.snr.2025.100282","DOIUrl":"10.1016/j.snr.2025.100282","url":null,"abstract":"<div><div>As a chronic disease, diabetes has impacted millions of individuals across the globe and is spreading at a very fast rate among adults mainly. Continuous observations of essential indicators like pulse rate, arterial pressure, and blood sugar levels, and oxygen saturation are crucial to maintain a healthy life and preventing severe complications associated with these. Using non-invasive method of blood glucose monitoring has paved its way to be one of the most prominent safest technique without harming the skin of the patients. This examination delves into the application of planar resonant sensors utilizing microwaves for quantifying glucose concentrations. The sensors based on RF EM waves mainly operating in the region from few MHz to GHz frequencies have garnered notable attention in the scientific community in recent years due to their significant contributions. They can detect alterations in dielectric properties caused by fluctuations in glucose concentrations. These sensors exhibit electrical reactions that rely on the dielectric properties of their surroundings. This review offers a current assessment of this sensing methodology, categorized by sensing parameters, and suggests typical non-invasive microwave sensor varieties for assessment. It further examines the key aspects of different sensor types, outlines potential future avenues, and addresses challenges in the field of glucose monitoring.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100282"},"PeriodicalIF":6.5,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a low-cost flexible potentiometric detector and its integrated system for electrochemical sensing of electrolytes in human sweat

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-01-17 DOI: 10.1016/j.snr.2025.100286

Chaojiang Li , Yuxuan Liu , Guodong Liu , Qifeng Tan , Xiaolong Dou , Yuting Xie , Xianchao Zhang

In the current era of wearable electrochemical sensors, there is a growing demand for a potentiometric detector that exhibits exceptional flexibility, comfortable wearability, and stability in electrolyte sensing. The present study designs and validates a low-cost (costs 17 dollars), small area (size of 4 × 5.8 cm²), highly flexible potentiometric detector, which is facilitated through the utilization of cost-effective components, flexible printed circuit boards, and Bluetooth low energy modules. Electrolyte electrochemical detection has been achieved by integrating wearable microfluidic electrochemical sensing devices and establishing wireless communication with smartphone applications. The efficacy of this designed flexible potentiometric detector is demonstrated in sodium and calcium ions detection, indicating comparable results to those obtained from commercial electrochemical workstations. By comparing the detection results with those obtained from ion chromatography, the flexible potentiometric detector is proved to be useful for a real-time, on-body quantitative analysis of various ion concentrations in human sweat.

{"title":"Development of a low-cost flexible potentiometric detector and its integrated system for electrochemical sensing of electrolytes in human sweat","authors":"Chaojiang Li , Yuxuan Liu , Guodong Liu , Qifeng Tan , Xiaolong Dou , Yuting Xie , Xianchao Zhang","doi":"10.1016/j.snr.2025.100286","DOIUrl":"10.1016/j.snr.2025.100286","url":null,"abstract":"<div><div>In the current era of wearable electrochemical sensors, there is a growing demand for a potentiometric detector that exhibits exceptional flexibility, comfortable wearability, and stability in electrolyte sensing. The present study designs and validates a low-cost (costs 17 dollars), small area (size of 4 × 5.8 cm<sup>2</sup>), highly flexible potentiometric detector, which is facilitated through the utilization of cost-effective components, flexible printed circuit boards, and Bluetooth low energy modules. Electrolyte electrochemical detection has been achieved by integrating wearable microfluidic electrochemical sensing devices and establishing wireless communication with smartphone applications. The efficacy of this designed flexible potentiometric detector is demonstrated in sodium and calcium ions detection, indicating comparable results to those obtained from commercial electrochemical workstations. By comparing the detection results with those obtained from ion chromatography, the flexible potentiometric detector is proved to be useful for a real-time, on-body quantitative analysis of various ion concentrations in human sweat.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100286"},"PeriodicalIF":6.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in the integration of isothermal nucleic acid amplification methods for point-of-care testing in resource-limited settings

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-01-17 DOI: 10.1016/j.snr.2025.100285

Noemi Bellassai , Roberta D'Agata , Giuseppe Spoto

Environmental conditions in low-resource areas exert significant pressure on populations, healthcare personnel, and the tools used for disease diagnosis. Fortunately, advancements in molecular assays now allow for rapid and efficient analysis, leading to effective clinical diagnostics and food safety interventions. Additionally, developing microfluidics and nanotechnologies has facilitated the miniaturization of integrated devices for point-of-care (POC) testing. These innovations have improved sensitivity while significantly reducing the time and cost associated with individual analyzes. This review focuses on the applications of molecular diagnostics for POC devices in low-resource settings, with a particular emphasis on isothermal nucleic acid amplification assays integrated into microfluidic platforms. It begins with an overview of the unmet needs for POC testing and its importance in resource-limited areas. Following this, isothermal amplification methods are described, highlighting their advantages and limitations when implemented in POC platforms, along with real-world applications in biological and food matrices in low- and middle-income countries. We then examine the unique features of POC systems, such as the use of low-cost materials for device fabrication, portability, and detection methods that provide rapid and reliable results near the site of patient care. This capability enables early-stage diagnosis and improved clinical management in low-resource environments.

{"title":"Advancements in the integration of isothermal nucleic acid amplification methods for point-of-care testing in resource-limited settings","authors":"Noemi Bellassai , Roberta D'Agata , Giuseppe Spoto","doi":"10.1016/j.snr.2025.100285","DOIUrl":"10.1016/j.snr.2025.100285","url":null,"abstract":"<div><div>Environmental conditions in low-resource areas exert significant pressure on populations, healthcare personnel, and the tools used for disease diagnosis. Fortunately, advancements in molecular assays now allow for rapid and efficient analysis, leading to effective clinical diagnostics and food safety interventions. Additionally, developing microfluidics and nanotechnologies has facilitated the miniaturization of integrated devices for point-of-care (POC) testing. These innovations have improved sensitivity while significantly reducing the time and cost associated with individual analyzes. This review focuses on the applications of molecular diagnostics for POC devices in low-resource settings, with a particular emphasis on isothermal nucleic acid amplification assays integrated into microfluidic platforms. It begins with an overview of the unmet needs for POC testing and its importance in resource-limited areas. Following this, isothermal amplification methods are described, highlighting their advantages and limitations when implemented in POC platforms, along with real-world applications in biological and food matrices in low- and middle-income countries. We then examine the unique features of POC systems, such as the use of low-cost materials for device fabrication, portability, and detection methods that provide rapid and reliable results near the site of patient care. This capability enables early-stage diagnosis and improved clinical management in low-resource environments.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100285"},"PeriodicalIF":6.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flexible touch and gesture recognition system for curved surfaces with machine learning for assistive applications

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-01-15 DOI: 10.1016/j.snr.2025.100284

Gitansh Verma , Shrutidhara Sarma , Eugen Koch , Andreas Dietzel

Touch is a fundamental mode of human-machine interaction and ability to monitor tactile pressure, recognize gestures and location of touch are crucial for touch-based technologies. However, achieving reliable touch sensing on curved surfaces remains challenging as flexing often disrupts the stability of sensor outputs and diminishes sensitivity, especially in dynamic environments. This study presents the development of a flexible multi-element touch sensing patch that can monitor its bending state as well as detect pressure with a sensitivity of 0.827 kPa⁻¹. The patch is fabricated using resistive strain sensors, screen printed onto a PET sheet with a foam backing. Evaluation electronics were integrated to ensure stable, noise-free signal acquisition, and output was processed with machine learning (ML) algorithms to classify gestures such as single and double finger taps, swipes, and touch locations, with 93 % accuracy, on both flat and curved surfaces. Based on the identified gesture, the system enables users to type text or control external devices with minimal physical effort. Its scalable fabrication, high sensitivity, mechanical resilience and seamless ML integration establishes it as a powerful and efficient tool for assistive technologies, designed to support individuals with limited speech and mobility, such as those with quadriplegia or paralysis.

{"title":"Flexible touch and gesture recognition system for curved surfaces with machine learning for assistive applications","authors":"Gitansh Verma , Shrutidhara Sarma , Eugen Koch , Andreas Dietzel","doi":"10.1016/j.snr.2025.100284","DOIUrl":"10.1016/j.snr.2025.100284","url":null,"abstract":"<div><div>Touch is a fundamental mode of human-machine interaction and ability to monitor tactile pressure, recognize gestures and location of touch are crucial for touch-based technologies. However, achieving reliable touch sensing on curved surfaces remains challenging as flexing often disrupts the stability of sensor outputs and diminishes sensitivity, especially in dynamic environments. This study presents the development of a flexible multi-element touch sensing patch that can monitor its bending state as well as detect pressure with a sensitivity of 0.827 kPa<sup>−1</sup>. The patch is fabricated using resistive strain sensors, screen printed onto a PET sheet with a foam backing. Evaluation electronics were integrated to ensure stable, noise-free signal acquisition, and output was processed with machine learning (ML) algorithms to classify gestures such as single and double finger taps, swipes, and touch locations, with 93 % accuracy, on both flat and curved surfaces. Based on the identified gesture, the system enables users to type text or control external devices with minimal physical effort. Its scalable fabrication, high sensitivity, mechanical resilience and seamless ML integration establishes it as a powerful and efficient tool for assistive technologies, designed to support individuals with limited speech and mobility, such as those with quadriplegia or paralysis.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100284"},"PeriodicalIF":6.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bipolar electrochemically generated fluorescence detector for microchip electrophoresis with and without a potentiostat: Application to reducible analyte detection

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-01-14 DOI: 10.1016/j.snr.2025.100283

Manjula B. Wijesinghe , Indika K. Warnakula , Dulan B. Gunasekara , Susan M. Lunte

Microchip electrophoresis (ME) is a separation method that makes it possible to quickly analyze multiple species in small volume samples in a miniaturized format. Two commonly employed detection approaches for ME are electrochemistry and fluorescence. Electrochemical detection can be employed for direct detection of analytes via redox chemistry, while fluorescence generally offers lower limits of detection but often requires analyte derivatization. The present study uses bipolar electrochemistry to couple the two detection methods into a single device as a detector for that encompasses the advantages of both techniques. This system employs a closed bipolar electrode (BPE) that converts the cathodic amperometric current produced at the separation (sensing) pole to a fluorescence response generated at the reporting pole. Two model analytes, benzoquinone and resazurin, were separated and detected using ME with reductive amperometric detection at the sensing pole of the BPE. A potentiostat was used to control the bipolar electrode. The response was then converted to fluorescence through the oxidation of 2,7-dichlorodihydrofluorescein in the reporting channel. This system was subsequently modified to run without a potentiostat by using the separation voltage to bias the BPE sensing pole. A voltage applied across the reporting channel was used to generate electroosmotic flow as well as bias the corresponding pole of the BPE. This potentiostat-free mode was evaluated for the determination of 3-nitro-l-tyrosine, a reducible biomarker of oxidative stress. Lastly, a sacrificial electrochemically active species, Trolox®, was incorporated into the reporting channel to compensate for background current at the sensing channel and lower the limits of detection.

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