Pub Date : 2025-01-08DOI: 10.1016/j.snr.2024.100275
Sadaf Maramizonouz , Jeremy J. Hawkes , Mohammad Rahmati , Yong-Qing Fu
In this study, the manipulation, patterning, and alignment of yeast particles were successfully demonstrated inside a continuous flow capillary bridge channel. A numerical model is presented to explore various frequencies and channel configurations, and the numerical results are compared to the experimental findings. The numerical model produced results in excellent agreement with the experimental data. Yeast particles were aligned in linear patterns while continuously flowing parallel to the air-water interfaces of the capillary bridge channel. The width of the fluid-guide significantly affected the acoustic pressure fields and thus the quality of particle patterning. Most frequencies can achieve particle alignment inside the capillary bridge channel, while the liquid flow straightens the particle line. Studies of ultrasound transfer from a solid wave-guide into the liquid in a capillary bridge channel have previously shown that some nodes which formed in the waveguide will cross the solid-fluid interface into the water of the capillary bridge. The nodes formed by this extension mechanism are not easily distinguishable from nodes formed by a resonance in the liquid since particles are attracted to them in the same way and the node-to-node separation is often similar. This paper provides further foundation for the new node extension concept and identifies some of its unique features.
{"title":"Continuous flow acoustofluidics in wall-less capillary bridge channels","authors":"Sadaf Maramizonouz , Jeremy J. Hawkes , Mohammad Rahmati , Yong-Qing Fu","doi":"10.1016/j.snr.2024.100275","DOIUrl":"10.1016/j.snr.2024.100275","url":null,"abstract":"<div><div>In this study, the manipulation, patterning, and alignment of yeast particles were successfully demonstrated inside a continuous flow capillary bridge channel. A numerical model is presented to explore various frequencies and channel configurations, and the numerical results are compared to the experimental findings. The numerical model produced results in excellent agreement with the experimental data. Yeast particles were aligned in linear patterns while continuously flowing parallel to the air-water interfaces of the capillary bridge channel. The width of the fluid-guide significantly affected the acoustic pressure fields and thus the quality of particle patterning. Most frequencies can achieve particle alignment inside the capillary bridge channel, while the liquid flow straightens the particle line. Studies of ultrasound transfer from a solid wave-guide into the liquid in a capillary bridge channel have previously shown that some nodes which formed in the waveguide will cross the solid-fluid interface into the water of the capillary bridge. The nodes formed by this extension mechanism are not easily distinguishable from nodes formed by a resonance in the liquid since particles are attracted to them in the same way and the node-to-node separation is often similar. This paper provides further foundation for the new node extension concept and identifies some of its unique features.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100275"},"PeriodicalIF":6.5,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-06DOI: 10.1016/j.snr.2025.100281
Yusuff Balogun , Ruoyu Yang , Gangli Wang
The formation of cluster aggregates in a (super)saturated solution prior to protein nucleation is crucial to overcoming the thermodynamic energy barrier which enables further growth of single crystals. This process is important for single crystal growth, separation and energy conversion among other important applications. For structural determination of biomacromolecules, neutron crystallography holds unique advantages in resolving hydrogen/proton over other structure determination techniques but faces technical obstacles in requiring large high-quality single crystals and preferentially hydrogen-deuterium exchanges. Herein, we explore protein nucleation in heavy water (D2O) via nanopore-based resistive pulse sensing, with lysozyme as prototype. By controlling localized supersaturation and phase transition at a nanopore through adjusting the potential waveform, a single protein crystal can be grown. Our focus is on understanding the translocation and/or transformation of protein aggregates through nanopores prior to the irreversible nucleation. As expected, higher protein concentrations tend to facilitate nucleation and growth of a single protein crystal with higher supersaturation, consistent with bulk experiments. At lower protein concentrations, individual current spikes are resolved as characteristic single-entity events in resistive pulse sensing. Those transient events are potential-dependent characterized by the peak amplitude, duration and area/charges. Statistical analysis reveals both translocation of protein oligomers and their transformation or further aggregation. This study represents the first step toward elucidating valuable insights into the dynamics of protein translocation and aggregation in heavy water and demonstrates the potential of using nanopores in the detection and characterization of dynamic phase transitions at single-event levels.
{"title":"Resistive pulse sensing of pre-nucleation activities during single-entity lysozyme crystallization on single nanopipettes","authors":"Yusuff Balogun , Ruoyu Yang , Gangli Wang","doi":"10.1016/j.snr.2025.100281","DOIUrl":"10.1016/j.snr.2025.100281","url":null,"abstract":"<div><div>The formation of cluster aggregates in a (super)saturated solution prior to protein nucleation is crucial to overcoming the thermodynamic energy barrier which enables further growth of single crystals. This process is important for single crystal growth, separation and energy conversion among other important applications. For structural determination of biomacromolecules, neutron crystallography holds unique advantages in resolving hydrogen/proton over other structure determination techniques but faces technical obstacles in requiring large high-quality single crystals and preferentially hydrogen-deuterium exchanges. Herein, we explore protein nucleation in heavy water (D<sub>2</sub>O) via nanopore-based resistive pulse sensing, with lysozyme as prototype. By controlling localized supersaturation and phase transition at a nanopore through adjusting the potential waveform, a single protein crystal can be grown. Our focus is on understanding the translocation and/or transformation of protein aggregates through nanopores prior to the irreversible nucleation. As expected, higher protein concentrations tend to facilitate nucleation and growth of a single protein crystal with higher supersaturation, consistent with bulk experiments. At lower protein concentrations, individual current spikes are resolved as characteristic single-entity events in resistive pulse sensing. Those transient events are potential-dependent characterized by the peak amplitude, duration and area/charges. Statistical analysis reveals both translocation of protein oligomers and their transformation or further aggregation. This study represents the first step toward elucidating valuable insights into the dynamics of protein translocation and aggregation in heavy water and demonstrates the potential of using nanopores in the detection and characterization of dynamic phase transitions at single-event levels.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100281"},"PeriodicalIF":6.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Breastmilk is an essential source of nutrients for newborn babies. It contains amino acids, nucleic acids and the essential immunological components required for the neonate to survive. Along with a number of other contaminants, it also includes several biomarkers, which are signs of a diseased state of the host. Thus, it is obvious that by examining the contents of this bodily fluid and how it affects the newborn, we can provide better healthcare solutions to both the mother and the child. There are several methods by which these analytes can be analysed, and they can be broadly classified in two categories; conventional and point-of-care based techniques. These approaches have benefits and drawbacks and are both in use today. However, point-of-care procedures are becoming increasingly popular in the scientific community because of their portability, affordability, and shorter turnaround times. In this review, the significance of employing these techniques to examine the intricacy of breastmilk as well as the contaminants and biomarkers that it contains will be covered. Wherever appropriate, the future of point-of-care approaches, including wearable sensors, has also been discussed, along with the significance, drawbacks, and advantages of combining these two technologies to recognize contaminants and biomarkers in breast milk.
{"title":"Recent advancements in Point-of-Care Detection of Contaminants and Biomarkers in Human Breast Milk: A comprehensive review","authors":"Shruti Janakiraman , Rinky Sha , Naresh Kumar Mani","doi":"10.1016/j.snr.2024.100280","DOIUrl":"10.1016/j.snr.2024.100280","url":null,"abstract":"<div><div>Breastmilk is an essential source of nutrients for newborn babies. It contains amino acids, nucleic acids and the essential immunological components required for the neonate to survive. Along with a number of other contaminants, it also includes several biomarkers, which are signs of a diseased state of the host. Thus, it is obvious that by examining the contents of this bodily fluid and how it affects the newborn, we can provide better healthcare solutions to both the mother and the child. There are several methods by which these analytes can be analysed, and they can be broadly classified in two categories; conventional and point-of-care based techniques. These approaches have benefits and drawbacks and are both in use today. However, point-of-care procedures are becoming increasingly popular in the scientific community because of their portability, affordability, and shorter turnaround times. In this review, the significance of employing these techniques to examine the intricacy of breastmilk as well as the contaminants and biomarkers that it contains will be covered. Wherever appropriate, the future of point-of-care approaches, including wearable sensors, has also been discussed, along with the significance, drawbacks, and advantages of combining these two technologies to recognize contaminants and biomarkers in breast milk.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100280"},"PeriodicalIF":6.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-26DOI: 10.1016/j.snr.2024.100273
Shamim Azimi , Maryam Moridsadat , Bhavin Shastri , Bruce W. Banfield , Carlos Escobedo , Aristides Docoslis
We present a novel plasmonic biosensing method for on-chip detection on viral particles featuring a microelectrode platform that integrates accelerated sampling of virus with surface-enhanced Raman scattering (SERS). We show experimentally that our approach can produce spectacular results owing to the unique incorporation of two key features: (1) Concentration amplification of virus on the SERS-active substrate; (2) Local plasmonic activity enhancement due to the targeted superimposition of silver nanoparticles on the captured virus sites. When tested for the detection of the M13 bacteriophage our “sandwich” assay yielded excellent reproducibility (signal variation <6 %) and a very low limit of detection (1.13 × 102 pfu/ml). Compared with the performance of our standard SERS substrates, SERS signals stronger by at least one order of magnitude are typically achieved. In addition to experimental results, our work also includes finite element (COMSOL Multiphysics) and finite-difference time-domain (FDTD) simulations that provide insights into the mechanisms of concentration amplification and plasmonic activity enhancement, respectively.
{"title":"Fast and sensitive virus detection using a plasmonic sensor that integrates electrokinetically assisted sampling and surface-enhanced Raman scattering","authors":"Shamim Azimi , Maryam Moridsadat , Bhavin Shastri , Bruce W. Banfield , Carlos Escobedo , Aristides Docoslis","doi":"10.1016/j.snr.2024.100273","DOIUrl":"10.1016/j.snr.2024.100273","url":null,"abstract":"<div><div>We present a novel plasmonic biosensing method for on-chip detection on viral particles featuring a microelectrode platform that integrates accelerated sampling of virus with surface-enhanced Raman scattering (SERS). We show experimentally that our approach can produce spectacular results owing to the unique incorporation of two key features: (1) Concentration amplification of virus on the SERS-active substrate; (2) Local plasmonic activity enhancement due to the targeted superimposition of silver nanoparticles on the captured virus sites. When tested for the detection of the M13 bacteriophage our “sandwich” assay yielded excellent reproducibility (signal variation <6 %) and a very low limit of detection (1.13 × 10<sup>2</sup> pfu/ml). Compared with the performance of our standard SERS substrates, SERS signals stronger by at least one order of magnitude are typically achieved. In addition to experimental results, our work also includes finite element (COMSOL Multiphysics) and finite-difference time-domain (FDTD) simulations that provide insights into the mechanisms of concentration amplification and plasmonic activity enhancement, respectively.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100273"},"PeriodicalIF":6.5,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-26DOI: 10.1016/j.snr.2024.100278
Sepideh Shafaei , Rahim Mohammad-Rezaei , Balal Khalilzadeh , Ibrahim Isildak
In this study, an efficient and ultrasensitive biosensor based on platinum nanoparticles decorated on MXene nanosheets (NSs) was reported for the detection of CA15–3 breast cancer biomarker. In order to increase the accuracy and reproducibility of the fabricated biosensor, MXene NSs and platinum nanoparticles (PtNPs) were electrochemically deposited on the surface of glassy carbon electrode (MXene-Pt/GCE). The synergistic effect of MXene and PtNPs caused an increased conductivity, fast electron transfers, amplified sensitivity and improved stabilization of streptavidin and CA15–3 antibody on the electrode surface. According to the electrochemical results, the electroactive surface area of MXene-Pt/GCE was obtained 0.1345 cm2 which was remarkably more than GCE. The detection limit and linear range for the developed CA15–3 breast cancer biosensor were 1 nU and 1 to 100 nU, respectively. Based on the data, the proposed biosensor exhibited unique reproducibility, stability, and selectivity which can be differentiate between the healthy serum samples and patients suffering from breast cancer.
{"title":"Platinum nanoparticles decorated on electrodeposited Ti3C2Tx MXene nanosheets as an ultrasensitive biosensor for CA15–3 detection","authors":"Sepideh Shafaei , Rahim Mohammad-Rezaei , Balal Khalilzadeh , Ibrahim Isildak","doi":"10.1016/j.snr.2024.100278","DOIUrl":"10.1016/j.snr.2024.100278","url":null,"abstract":"<div><div>In this study, an efficient and ultrasensitive biosensor based on platinum nanoparticles decorated on MXene nanosheets (NSs) was reported for the detection of CA15–3 breast cancer biomarker. In order to increase the accuracy and reproducibility of the fabricated biosensor, MXene NSs and platinum nanoparticles (PtNPs) were electrochemically deposited on the surface of glassy carbon electrode (MXene-Pt/GCE). The synergistic effect of MXene and PtNPs caused an increased conductivity, fast electron transfers, amplified sensitivity and improved stabilization of streptavidin and CA15–3 antibody on the electrode surface. According to the electrochemical results, the electroactive surface area of MXene-Pt/GCE was obtained 0.1345 cm<sup>2</sup> which was remarkably more than GCE. The detection limit and linear range for the developed CA15–3 breast cancer biosensor were 1 nU and 1 to 100 nU, respectively. Based on the data, the proposed biosensor exhibited unique reproducibility, stability, and selectivity which can be differentiate between the healthy serum samples and patients suffering from breast cancer.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100278"},"PeriodicalIF":6.5,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Recirculating Aquaculture Systems (RAS) have revolutionized the protein production sector in aquaculture, leading to significant growth and expansion of the industry. Despite the success of RAS in aquaculture, there are challenges related to stress in fish raised in these systems, which can impact their food intake, growth, and overall well-being. One of the major limitations in the aquaculture industry is the lack of smart sensors for real-time detection of stress hormones like cortisol, hindering our ability to understand and effectively manage the welfare of fish in these systems. In this work, a graphene oxide (GO) coated long period grating (LPG) was fabricated into a double-clad optical fiber (DCF) with W-shaped refractive index profile. The working point of the device was tuned to the mode transition region to enhance its sensitivity against outer medium changes. It was further integrated into a microfluidic system and the fiber surface was functionalized with specific anti-cortisol antibodies for the detection of cortisol. Finally, the performance of this immunosensor was evaluated for a cortisol concentration range of 0.01 ng/mL to 100 ng/mL, a wide working range of concentrations of relevant interest, achieving a limit of detection (LOD) of 0.06 ng/mL. Moreover, a selectivity test using testosterone and glucose as interfering substances was carried out.
{"title":"Cortisol detection using a Long Period Fiber Grating Immunosensor coated with Graphene Oxide","authors":"Simone Soares , Ambra Giannetti , Flavio Esposito , Lucia Sansone , Anubhav Srivastava , Stefania Campopiano , Michele Giordano , Margarida Facão , Nuno F. Santos , Agostino Iadicicco , Carlos Marques , Francesco Chiavaioli","doi":"10.1016/j.snr.2024.100279","DOIUrl":"10.1016/j.snr.2024.100279","url":null,"abstract":"<div><div>Recirculating Aquaculture Systems (RAS) have revolutionized the protein production sector in aquaculture, leading to significant growth and expansion of the industry. Despite the success of RAS in aquaculture, there are challenges related to stress in fish raised in these systems, which can impact their food intake, growth, and overall well-being. One of the major limitations in the aquaculture industry is the lack of smart sensors for real-time detection of stress hormones like cortisol, hindering our ability to understand and effectively manage the welfare of fish in these systems. In this work, a graphene oxide (GO) coated long period grating (LPG) was fabricated into a double-clad optical fiber (DCF) with W-shaped refractive index profile. The working point of the device was tuned to the mode transition region to enhance its sensitivity against outer medium changes. It was further integrated into a microfluidic system and the fiber surface was functionalized with specific anti-cortisol antibodies for the detection of cortisol. Finally, the performance of this immunosensor was evaluated for a cortisol concentration range of 0.01 ng/mL to 100 ng/mL, a wide working range of concentrations of relevant interest, achieving a limit of detection (LOD) of 0.06 ng/mL. Moreover, a selectivity test using testosterone and glucose as interfering substances was carried out.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100279"},"PeriodicalIF":6.5,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Here, we present a diagnostic on a chip platform based on laser-induced graphene (LIG) electrochemical immunosensors for serological detection. The electrochemical immunosensors are fabricated through CO2 laser induction on polyimide (PI) sheets. Optimal electrochemical activity of LIG electrodes is obtained under optimized conditions of laser fluence. To verify the application, the serological detection platform was demonstrated. After functionalization with dengue virus (DENV) antigen, the LIG electrochemical immunosensors are able to sense the presence of mouse anti-flavivirus monoclonal (4G2) antibody in a wide linear working range of 25–20,000 ng/ml with the limit of detection (LOD) of 17.41 ng/ml. A specific recognition with 4G2 antibodies against with media protein and isotype is confirmed. Furthermore, the reliability of LIG electrochemical immunosensors compared to conventional enzyme-linked immunosorbent assay (ELISA) is verified through the NS1 antibodies identification in human blood serum clinical samples at room temperature. Our results highlight that the LIG-based electrode is a promising platform for electrochemical immunosensors, aimed at developing reliable and practical diagnostic tools for serological detection. These tools enable early diagnosis of infectious diseases, as well as non-invasive and rapid screening.
{"title":"Laser-induced graphene electrochemical immunosensors for rapid and sensitive serological detection: A case study on dengue detection platform","authors":"Supawee Inlumphan , Winadda Wongwiriyapan , Narathon Khemasiri , Prapakorn Rattanawarinchai , Piyawan Leepheng , Panuwat Luengrojanakul , Tuksadon Wuttikhun , Michiko Obata , Masatsugu Fujishige , Kenji Takeuchi , Mayuree Phonyiem Reilly , Teerayut Uwanno , Mati Horprathum , Supanit Porntheeraphat , Kannika Sitthisuwannakul , Supranee Phanthanawiboon , Annop Klamchuen","doi":"10.1016/j.snr.2024.100276","DOIUrl":"10.1016/j.snr.2024.100276","url":null,"abstract":"<div><div>Here, we present a diagnostic on a chip platform based on laser-induced graphene (LIG) electrochemical immunosensors for serological detection. The electrochemical immunosensors are fabricated through CO<sub>2</sub> laser induction on polyimide (PI) sheets. Optimal electrochemical activity of LIG electrodes is obtained under optimized conditions of laser fluence. To verify the application, the serological detection platform was demonstrated. After functionalization with dengue virus (DENV) antigen, the LIG electrochemical immunosensors are able to sense the presence of mouse anti-flavivirus monoclonal (4G2) antibody in a wide linear working range of 25–20,000 ng/ml with the limit of detection (LOD) of 17.41 ng/ml. A specific recognition with 4G2 antibodies against with media protein and isotype is confirmed. Furthermore, the reliability of LIG electrochemical immunosensors compared to conventional enzyme-linked immunosorbent assay (ELISA) is verified through the NS1 antibodies identification in human blood serum clinical samples at room temperature. Our results highlight that the LIG-based electrode is a promising platform for electrochemical immunosensors, aimed at developing reliable and practical diagnostic tools for serological detection. These tools enable early diagnosis of infectious diseases, as well as non-invasive and rapid screening.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100276"},"PeriodicalIF":6.5,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-20DOI: 10.1016/j.snr.2024.100277
Muhammad Asraf Mansor , Muhammad Asyraf Jamrus , Chong Kar Lok , Mohd Ridzuan Ahmad , Michal Petrů , Seyed Saeid Rahimian Koloor
Microfluidic separation of particles and cells has been extensively developed in recent years in a wide range of applications of sciences, engineering, and industry. At the same time, this area has been explored within the framework of interdisciplinary study, resulting in several experiments conducted in this field. Due to the heterogeneity of cells, label-free methods that utilize the inherent physical properties of cells to separate, offer more advantages including high throughput, simple design, reduced sample volumes, and low device cost. This paper comprehensively studies the latest progress in microfluidic technology for both active and passive cell separation techniques. In order to limit the extent of this work, our primary emphasis is on six common methods of cell separation: acoustophoresis, dielectrophoresis, magnetophoresis, inertial, pinched flow fractionation (PFF), and deterministic lateral displacement (DLD). We elaborate on the efficiency, throughput rate, and clogging in microfluidic devices. In addition, the advantages and limitations of both microfluidics devices were discussed.
{"title":"Microfluidic device for both active and passive cell separation techniques: A review","authors":"Muhammad Asraf Mansor , Muhammad Asyraf Jamrus , Chong Kar Lok , Mohd Ridzuan Ahmad , Michal Petrů , Seyed Saeid Rahimian Koloor","doi":"10.1016/j.snr.2024.100277","DOIUrl":"10.1016/j.snr.2024.100277","url":null,"abstract":"<div><div>Microfluidic separation of particles and cells has been extensively developed in recent years in a wide range of applications of sciences, engineering, and industry. At the same time, this area has been explored within the framework of interdisciplinary study, resulting in several experiments conducted in this field. Due to the heterogeneity of cells, label-free methods that utilize the inherent physical properties of cells to separate, offer more advantages including high throughput, simple design, reduced sample volumes, and low device cost. This paper comprehensively studies the latest progress in microfluidic technology for both active and passive cell separation techniques. In order to limit the extent of this work, our primary emphasis is on six common methods of cell separation: acoustophoresis, dielectrophoresis, magnetophoresis, inertial, pinched flow fractionation (PFF), and deterministic lateral displacement (DLD). We elaborate on the efficiency, throughput rate, and clogging in microfluidic devices. In addition, the advantages and limitations of both microfluidics devices were discussed.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100277"},"PeriodicalIF":6.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-17DOI: 10.1016/j.snr.2024.100274
Mingchuan Wang , Shiya Lv , Yu Wang , Zhaojie Xu , Peiyao Jiao , Yu Liu , Siyu Zhang , Yirong Wu , Mixia Wang , Jinping Luo , Shi Yan , Zhimei Qi , Yilin Song , Juntao Liu , Xinxia Cai
Spatial cognition is crucial for animal survival and reflects higher-order cognitive processes achieved through the coordination of neurons, local networks, and the brain's overall structure. Neural biosensors reliably detect signals in the brain at multiple levels, including neuronal action potentials (spikes), local field potentials (LFP), and blood-oxygen-level-dependent (BOLD) signals, which are interconnected yet distinct. These signals have significantly contributed to spatial cognition research. However, a comprehensive analysis across these levels is lacking, limiting full understanding of neural encoding and decoding mechanisms. This review introduces biosensor methods for detecting neural signals at various levels and discusses their applications in spatial cognition. It aims to inspire future research by promoting multi-modal, multi-level approaches for comprehensive decoding of neural mechanisms.
{"title":"Advances in neural information detection sensors for spatial cognition research: A review","authors":"Mingchuan Wang , Shiya Lv , Yu Wang , Zhaojie Xu , Peiyao Jiao , Yu Liu , Siyu Zhang , Yirong Wu , Mixia Wang , Jinping Luo , Shi Yan , Zhimei Qi , Yilin Song , Juntao Liu , Xinxia Cai","doi":"10.1016/j.snr.2024.100274","DOIUrl":"10.1016/j.snr.2024.100274","url":null,"abstract":"<div><div>Spatial cognition is crucial for animal survival and reflects higher-order cognitive processes achieved through the coordination of neurons, local networks, and the brain's overall structure. Neural biosensors reliably detect signals in the brain at multiple levels, including neuronal action potentials (spikes), local field potentials (LFP), and blood-oxygen-level-dependent (BOLD) signals, which are interconnected yet distinct. These signals have significantly contributed to spatial cognition research. However, a comprehensive analysis across these levels is lacking, limiting full understanding of neural encoding and decoding mechanisms. This review introduces biosensor methods for detecting neural signals at various levels and discusses their applications in spatial cognition. It aims to inspire future research by promoting multi-modal, multi-level approaches for comprehensive decoding of neural mechanisms.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100274"},"PeriodicalIF":6.5,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Herein, ZnS:Ni quantum dots embedded within carbon nanofibers (ZnS:Ni/CNFs) are synthesized via a simple electrospinning technology with a subsequent carbonization process and proposed for biosensing purposes. This hybrid material was applied as a substrate for aptamer immobilization aiming at Trypsin sensing. It is revealed that the electrochemical Trypsin aptasensor developed based on the novel ZnS:Ni/CNFs composite exhibited superior analysis performance to previously reported Trypsin aptasensor, such as a wide linear range from 0.1 fg mL−1 to 600 ng mL−1 and low detection limit of 0.03 fg mL−1 (S/N = 3), excellent reproducibility and selectivity. In addition, apt/ ZnS:Ni CNFs/GCE can detect Trypsin present in human serum samples. This work can inspire researchers to build functional hybrid nanomaterials based on nanofibers for the purpose of generating electrochemical biosensors. Additionally, the implementation of the suggested platform has the potential to be highly beneficial in future studies and extends their applications in biosensors and health monitoring.
{"title":"Electrospinning ZnS:Ni quantum dots into carbon nanofibrous structure as a base for the electrochemical aptasensor for detection of trypsin","authors":"Haniyeh Shantiaei, Mahmoud Roushani, Farzaneh Mohammadi","doi":"10.1016/j.snr.2024.100272","DOIUrl":"10.1016/j.snr.2024.100272","url":null,"abstract":"<div><div>Herein, ZnS:Ni quantum dots embedded within carbon nanofibers (ZnS:Ni/CNFs) are synthesized via a simple electrospinning technology with a subsequent carbonization process and proposed for biosensing purposes. This hybrid material was applied as a substrate for aptamer immobilization aiming at Trypsin sensing. It is revealed that the electrochemical Trypsin aptasensor developed based on the novel ZnS:Ni/CNFs composite exhibited superior analysis performance to previously reported Trypsin aptasensor, such as a wide linear range from 0.1 fg mL<sup>−1</sup> to 600 ng mL<sup>−1</sup> and low detection limit of 0.03 fg mL<sup>−1</sup> (S/<em>N</em> = 3), excellent reproducibility and selectivity. In addition, apt/ ZnS:Ni CNFs/GCE can detect Trypsin present in human serum samples. This work can inspire researchers to build functional hybrid nanomaterials based on nanofibers for the purpose of generating electrochemical biosensors. Additionally, the implementation of the suggested platform has the potential to be highly beneficial in future studies and extends their applications in biosensors and health monitoring.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100272"},"PeriodicalIF":6.5,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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