Sensors and Actuators Reports最新文献_第6页

A high-temperature heat flux sensor using the transverse Seebeck effect in elemental rhenium 单质铼中利用横向塞贝克效应的高温热流传感器

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-10-08 DOI: 10.1016/j.snr.2025.100391

Kenneth McAfee , Peter B. Sunderland , Oded Rabin

Heat flux sensors compatible with hot environments are critical to advance aerospace, materials, and energy generation technologies that cope with extreme thermal conditions. In this work, we report on the development and characterization of a high-temperature heat flux sensor using the transverse Seebeck effect in rhenium single crystals. The sensor leverages refractory alloys and ceramics compatible with temperatures exceeding 1000 °C. The heat flux sensor was characterized from room temperature to 500 °C using a temperature-controlled calibration facility. At constant temperature, the sensor’s voltage output is linear with respect to the absorbed heat flux. The responsivity of the sensor varies with temperature, from 1.3

μ

V/(W/cm

^{2}

) at room temperature to −3.2

μ

V/(W/cm

^{2}

) at 500 °C, increasing monotonically in magnitude after changing sign from positive to negative at approximately 300 °C. The experimental results are in good agreement with analytical predictions of the sensor’s temperature-dependent responsivity, which suggest a further increase in magnitude up to −7.4

μ

V/(W/cm

^{2}

) at 1000 °C. These results highlight the unique characteristics of rhenium as a TSE transducer. The design offers compatibility with a wide range of operating temperatures and yields a measurement sensitivity that increases as the environmental conditions become more challenging.

与热环境兼容的热流传感器对于应对极端热条件的先进航空航天、材料和能源生产技术至关重要。在这项工作中，我们报告了利用铼单晶中的横向塞贝克效应开发和表征高温热流通量传感器。该传感器利用耐火合金和陶瓷兼容温度超过1000°C。热通量传感器在室温至500°C范围内使用温控校准设备进行了表征。在恒定温度下，传感器的电压输出与吸收的热通量成线性关系。传感器的响应度随温度变化，从室温时的1.3 μV/(W/cm2)到500℃时的- 3.2 μV/(W/cm2)，在约300℃时由正变为负，其幅度单调增加。实验结果与传感器的温度相关响应性的分析预测非常吻合，表明在1000°C时，其幅度进一步增加至- 7.4 μV/(W/cm2)。这些结果突出了铼作为TSE换能器的独特特性。该设计具有广泛的工作温度范围的兼容性，并且随着环境条件变得更具挑战性，测量灵敏度也会增加。

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引用次数: 0

In-situ spectroelectrochemical insights into the EC’ mechanism: NADH oxidation catalyzed by an osmium complex 原位光谱电化学洞察EC的机制：由锇络合物催化的NADH氧化

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-10-06 DOI: 10.1016/j.snr.2025.100392

Seokjin Oh , Eun Joong Kim , Chung Mu Kang , Donghoon Han

In-situ analytical techniques serve as powerful tools for visualizing reaction processes. Spectroelectrochemical methods, which integrate spectroscopy with electrochemistry, provide critical insights into electrochemical reactions. Molecules that undergo spectral changes due to oxidation- or reduction-induced alterations in their electronic structures can be monitored in real time, enabling direct confirmation of redox processes. In this study, ultraviolet-visible (UV-vis) absorption spectroscopy was combined with electrochemical analysis to investigate potential-dependent absorbance changes associated with the oxidation of NADH in the presence of an osmium complex serving as an electron transfer mediator. A distinct decrease in NADH-related absorbance was observed at potentials lower than its intrinsic onset potential, attributable to the catalytic activity of the mediator. These findings provide direct visual evidence for an EC’ (electrochemical-catalytic) reaction mechanism, wherein the mediator facilitates electron transfer by promoting NADH oxidation at reduced overpotentials.

原位分析技术是可视化反应过程的有力工具。光谱电化学方法将光谱学与电化学相结合，为电化学反应提供了重要的见解。由于氧化或还原引起的电子结构改变而发生光谱变化的分子可以实时监测，从而直接确认氧化还原过程。在这项研究中，紫外-可见（UV-vis）吸收光谱与电化学分析相结合，研究了在作为电子传递介质的锇络合物存在下NADH氧化相关的电位依赖性吸光度变化。由于介质的催化活性，在电位低于其内在起始电位时，观察到nadh相关吸光度明显下降。这些发现为EC（电化学催化）反应机制提供了直接的视觉证据，其中介质通过在降低过电位下促进NADH氧化来促进电子转移。

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引用次数: 0

Nanoscale heterojunction indium oxide/molybdenum disulphide field-effect transistor: A cost-effective wafer scale fabrication with improved performance 纳米异质结氧化铟/二硫化钼场效应晶体管：具有改进性能的低成本晶圆级制造

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-10-02 DOI: 10.1016/j.snr.2025.100390

Mohamed Taha Amen, Cheah Edward, Duy Phu Tran, Benjamin Thierry

Metal oxide heterojunction thin films are promising building blocks for the fabrication of functional devices in microelectronics, bio-chemical sensors, photovoltaics, and optical displays. However, balancing the large-scale manufacturability with performance, uniformity, and cost-effectiveness remains a significant challenge. Here, we report a wafer-scale fabrication process of bilayer stacks of high-mobility indium oxide and molybdenum disulphide heterojunction thin films and their application for the preparation of high-performance field-effect transistors (FETs). The annealed heterojunction thin film exhibits uniform crystalline structures and good surface roughness across the whole wafer. A simple soft lithography and lift-off process of the heterojunction thin film could produce nanotransistor devices with a remarkable electron mobility enhancement of more than 1100 % compared to indium oxide or molybdenum disulphide single layer devices. The heterojunction FET sensors yielded more than a twofold higher pH sensitivity compared to silicon-based ionic FETs and excellent linearity. These findings coupled with the cost-effective fabrication strategy underscore the potential of indium oxide and molybdenum disulphide heterojunction FET devices.

金属氧化物异质结薄膜是制造微电子、生化传感器、光伏和光学显示器等功能器件的有前途的基石。然而，平衡大规模可制造性与性能、一致性和成本效益仍然是一个重大挑战。本文报道了一种高迁移率氧化铟和二硫化钼异质结薄膜双层堆叠的晶圆级制备工艺及其在高性能场效应晶体管（fet）制备中的应用。退火后的异质结薄膜在整个晶圆上具有均匀的晶体结构和良好的表面粗糙度。与氧化铟或二硫化钼单层器件相比，异质结薄膜的简单软光刻和剥离工艺可以生产出电子迁移率提高1100%以上的纳米晶体管器件。与硅基离子FET相比，异质结FET传感器的pH灵敏度提高了两倍以上，线性度也很好。这些发现加上具有成本效益的制造策略，强调了氧化铟和二硫化钼异质结FET器件的潜力。

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引用次数: 0

Ternary Prussian blue nanozymes with synergistic multimetallic catalysis enable ultrasensitive lateral flow immunoassay for respiratory syncytial virus diagnosis 具有协同多金属催化作用的三元普鲁士蓝纳米酶可用于呼吸道合胞病毒诊断的超灵敏侧流免疫测定

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-09-29 DOI: 10.1016/j.snr.2025.100389

Dinglin Fan , Zhen Ren , Yongli Li , Xukui Li , Zhipiao Tian , Wei Huang , Lin Zhan , Shijia Ding , Yongjie Xu , Fuxun Yu

Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infections in infants and children, and early and accurate diagnosis is crucial for its effective treatment and disease management. Here, we developed a nanozyme-enhanced lateral flow immunoassay (LFIA) that employed a newly generated monoclonal antibody against the conserved RSV nucleocapsid (N) protein. The platform was based on a ternary metal–organic framework nanozyme composed of iron, cobalt, and nickel ions, which exhibited synergistically enhanced peroxidase-like activity. This nanozyme efficiently catalyzed the oxidation of 3,3′ -diaminobenzidine, producing a colorimetric signal on the LFIA strip for visual detection. Due to the catalytic enhancement, the assay achieved a detection limit of 5 pg/mL for recombinant RSV N protein. For live RSV, the biosensor demonstrated a detection threshold of 10^0.875 TCID50/mL, representing a 1000-fold sensitivity improvement over AuNP-based LFIA. Moreover, the strip demonstrated high specificity and no cross-reactivity with other viruses. Owing to its simplicity, speed, and efficiency, the developed LFIA offers a robust point-of-care solution for RSV diagnosis. This platform holds great potential for clinical screening and early detection of viral infections, underscoring its value in future pandemic preparedness and respiratory disease surveillance.

呼吸道合胞病毒（RSV）是婴幼儿下呼吸道感染的主要病因，早期准确诊断对其有效治疗和疾病管理至关重要。在这里，我们开发了一种纳米酶增强的侧流免疫测定（LFIA），该方法使用了一种新生成的针对保守的RSV核衣壳(N)蛋白的单克隆抗体。该平台基于由铁、钴和镍离子组成的三元金属-有机框架纳米酶，具有协同增强的过氧化物酶样活性。该纳米酶有效催化3,3 ' -二氨基联苯胺氧化，在LFIA条带上产生比色信号，用于视觉检测。由于催化增强，该方法对重组RSV N蛋白的检测限为5 pg/mL。对于活RSV，该生物传感器的检测阈值为100.875 TCID50/mL，比基于aunp的LFIA灵敏度提高了1000倍。该条带具有高特异性，与其他病毒无交叉反应性。由于其简单，快速和高效，开发的LFIA为RSV诊断提供了一个强大的护理点解决方案。该平台在临床筛查和早期发现病毒感染方面具有巨大潜力，强调了其在未来大流行防范和呼吸道疾病监测方面的价值。

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引用次数: 0

Centrifugal microfluidic systems for cancer cell separation: Advances, challenges, and applications 用于癌细胞分离的离心微流控系统：进展、挑战和应用

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-09-26 DOI: 10.1016/j.snr.2025.100387

Alireza Farahinia , Wenjun Zhang , Ildiko Badea

Cancer remains a leading global health challenge, with circulating tumor cells (CTCs) playing a pivotal role in metastasis and disease progression. Efficient detection and isolation of CTCs are essential for early diagnosis, therapeutic monitoring, and the advancement of personalized treatment strategies. However, their extreme rarity in peripheral blood presents significant technical challenges for reliable enrichment and analysis. Centrifugal microfluidic systems, or lab-on-a-disc (LOCD) platforms, offer a promising solution by enabling automated, high-throughput, and cost-effective separation of rare cancer cells with minimal manual intervention. This review provides a comprehensive analysis of recent advances in centrifugal microfluidic technologies, with a focus on cancer cell separation for diagnostics and the challenges of clinical translation. Particular attention is given to the optimization of separation techniques, improvements in microchannel design, and strategies to minimize contamination and cell damage while enhancing purity and yield. We critically compare label-free, affinity-based, and hybrid separation approaches, and examine how material selection, surface functionalization, automation, and integrated detection modules in-fluence device performance. Clinical relevance is emphasized throughout, including examples of real patient applications, regulatory challenges, and translational barriers. Furthermore, we propose future directions to address persistent limitations such as clogging, limited specificity, and standardization. While routine clinical implementation remains complex, recent innovations have significantly improved system robustness, reproducibility, and accessibility. This review serves as a resource for researchers and clinicians, summarizing the current state of the field and outlining the path forward for the next generation of centrifugal microfluidic systems tailored for cancer cell separation.

癌症仍然是一个主要的全球健康挑战，循环肿瘤细胞（ctc）在转移和疾病进展中起着关键作用。有效检测和分离CTCs对于早期诊断、治疗监测和个性化治疗策略的推进至关重要。然而，它们在外周血中极其罕见，为可靠的富集和分析带来了重大的技术挑战。离心式微流体系统，或碟上实验室（lod）平台，提供了一个很有前途的解决方案，通过最少的人工干预，实现罕见癌细胞的自动化、高通量和经济高效的分离。本文综述了离心微流控技术的最新进展，重点介绍了用于诊断的癌细胞分离和临床转化的挑战。特别关注分离技术的优化，微通道设计的改进，以及在提高纯度和产量的同时最大限度地减少污染和细胞损伤的策略。我们批判性地比较了无标签、基于亲和力和混合分离方法，并研究了材料选择、表面功能化、自动化和集成检测模块如何影响设备性能。临床相关性强调贯穿始终，包括实际患者应用，监管挑战和翻译障碍的例子。此外，我们提出了未来的方向，以解决持续的限制，如堵塞，有限的特异性和标准化。虽然常规临床实施仍然很复杂，但最近的创新显著提高了系统的稳健性、可重复性和可及性。本综述为研究人员和临床医生提供了资源，总结了该领域的现状，并概述了为癌细胞分离量身定制的下一代离心微流控系统的前进道路。

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引用次数: 0

Interplay of surface nanotopography and tether flexibility towards analyte capture by tethered receptors 表面纳米形貌和系绳弹性对系绳受体捕获分析物的相互作用

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-09-26 DOI: 10.1016/j.snr.2025.100388

Matteo Beggiato, Hugo Payen, Sivashankar Krishnamoorthy

Engineering topography and chemistry at the molecular length scale holds significant potential to control analyte binding at the biosensing interface. Here, we investigate the influence of nanotopography on analyte capture, by receptors tethered to the sensor surface via rigid or flexible tethers. Two different scenarios for analyte binding are investigated, viz. gold nanoparticles (as pseudo analyte) to aminated surfaces and neutravidin to biotinylated surfaces. The findings reveal the impact of nanotopography to be different depending on whether the amine or biotin receptors were bound to surface via rigid or flexible tethers. In case of rigid tethers, the nanotopography was found to have an influence beyond what could be attributed to the increase in surface areas. When flexible tethers were used, the increase in analyte capture could be attributed to the increase in surface areas as well as an increase in analyte capture efficiencies due to flexible tethers. The findings highlight the nanotopography and tether flexibility as having unique potential to maximize analyte capture efficiencies on affinity biosensors. The use of nano-QCM sensors exhibiting well-defined, periodic nanotopographies proved indispensable to real-time, label-free quantification of analyte⬄nanostructure interactions at the nano biosensing interface.

分子长度尺度上的工程地形和化学对于控制生物传感界面上分析物的结合具有重要的潜力。在这里，我们研究了纳米形貌对分析物捕获的影响，通过刚性或柔性绳系在传感器表面的受体。研究了分析物结合的两种不同情况，即金纳米颗粒（作为伪分析物）与胺化表面结合，中性蛋白与生物素化表面结合。研究结果表明，纳米形貌的影响取决于胺或生物素受体是通过刚性还是柔性系链结合在表面上。在刚性系索的情况下，发现纳米形貌的影响超出了可以归因于表面积增加的影响。当使用柔性系绳时，分析物捕获的增加可归因于表面积的增加以及由于柔性系绳而增加的分析物捕获效率。研究结果强调，纳米形貌和系绳灵活性具有独特的潜力，可以最大限度地提高亲和生物传感器的分析物捕获效率。纳米qcm传感器具有良好定义的周期性纳米形貌，对于实时、无标记定量分析分析物⬄纳米结构相互作用在纳米生物传感界面是必不可少的。

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引用次数: 0

Recent advances in sensing technologies for fentanyl and its analogs: Challenges and future directions 芬太尼及其类似物传感技术的最新进展：挑战与未来方向

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-09-23 DOI: 10.1016/j.snr.2025.100386

Farbod Shirinichi, Yanjing Gao, Matthew J. Webber, Yichun Wang

Fentanyl is a highly potent synthetic opioid which, along with structurally related analogs like carfentanil, is a significant driver of the global epidemic of opioid abuse. These compounds are often added in small amounts to other illicit drugs, found in misrepresented samples, or introduced through unintentional cross-contamination. Their low concentration, structural diversity, extreme potency, and occurrence in complex matrices like biological fluids increase the risk of fatal overdose and complicate their detection. The development of accurate, sensitive, and selective detection technologies is therefore crucial for applications in clinical diagnostics, forensics, public health surveillance, and law enforcement. This review discusses advanced sensors and analytical techniques for identifying fentanyl and its analogs, including colorimetric assays, immunoassays, electrochemical sensors, Raman spectroscopy, liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC–MS), nuclear magnetic resonance spectroscopy (NMR), and Fourier-transform infrared spectroscopy (FTIR). Each method is evaluated on the basis of its sensitivity, selectivity, ease of use, compatibility with complex matrices, stability, and ability to distinguish fentanyl from related analogs and other drugs of abuse. By detailing current advances and limitations in detection methodologies, this comprehensive overview of the field of fentanyl detection offers insights to guide the development of more effective and practical technologies tailored for diverse analytical and research settings.

芬太尼是一种强效的合成阿片类药物，与卡芬太尼等结构相关的类似物一起，是全球阿片类药物滥用流行的重要驱动因素。这些化合物通常被少量添加到其他非法药物中，在错误的样品中发现，或通过无意的交叉污染引入。它们的低浓度、结构多样性、极强的效力以及在生物液体等复杂基质中的存在增加了致命过量的风险，并使其检测复杂化。因此，开发准确、敏感和有选择性的检测技术对于临床诊断、法医学、公共卫生监测和执法中的应用至关重要。本文综述了用于鉴定芬太尼及其类似物的先进传感器和分析技术，包括比色法、免疫分析法、电化学传感器、拉曼光谱、液相色谱-质谱法（LC-MS）、气相色谱-质谱法（GC-MS）、核磁共振光谱法（NMR）和傅里叶变换红外光谱法（FTIR）。每一种方法都是根据其灵敏度、选择性、易用性、与复杂基质的相容性、稳定性以及区分芬太尼与相关类似物和其他滥用药物的能力来评估的。通过详细介绍检测方法的当前进展和局限性，本文对芬太尼检测领域的全面概述提供了见解，以指导针对各种分析和研究环境量身定制的更有效和实用的技术的发展。

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引用次数: 0

Machine learning in cancer prognostic and diagnostic biomarkers: A promising approach for early cancer detection 癌症预后和诊断生物标志物中的机器学习：早期癌症检测的一种有前途的方法

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-09-17 DOI: 10.1016/j.snr.2025.100385

Pegah Vosoughi , Seyed Morteza Naghib , Ghasem Takdehghan

Cancer is a multifaceted disease that arises from both genetic and epigenetic alterations that disturb the regulation of cell growth and programmed cell death, also known as apoptosis. This imbalance results in unchecked cell proliferation, ultimately leading to tumor formation. Globally, cancer presents a significant public health issue, causing millions of deaths each year and placing immense pressure on healthcare systems around the world. Early cancer detection is crucial, as it dramatically improves the chances of successful treatment. Consequently, advancements in biomarker research and diagnostic technologies are vital for creating more effective detection strategies that reduce the impact of cancer on patients and healthcare systems. For biomarkers to be helpful in clinical practice, they must thoroughly evaluate their analytical and clinical validity and utility. Analytical validity focuses on the technical accuracy of biomarker assays, encompassing aspects such as sample handling, assay methods, and the reliability of the results. In recent years, machine learning (ML) and deep learning (DL) have become crucial tools for identifying biomarkers in healthcare engineering. The advancements in artificial intelligence (AI) have expanded these technologies' applications across various healthcare domains. This research highlights the latest innovations and methodologies, including advanced feature selection techniques and ML/DL algorithms. This review emphasizes the transformative impact of ML technologies in biomarker discovery. It also encompasses multiple facets of ML-based platforms, detailing their applications and effectiveness in biomarker discovery. Using sophisticated algorithms, these advanced computational methods allow researchers to analyze various data types, from genomic sequences to proteomic profiles. This approach facilitates the identification of novel biomarkers, enhances our understanding of cancer biology, and paves the way for improved patient care through personalized medicine. This review highlights exciting opportunities for future research, encouraging continuous innovation and collaboration across disciplines among data scientists, healthcare experts, and academic researchers.

癌症是一种多方面的疾病，由遗传和表观遗传改变引起，这些改变扰乱了细胞生长和程序性细胞死亡（也称为细胞凋亡）的调节。这种不平衡导致不受控制的细胞增殖，最终导致肿瘤的形成。在全球范围内，癌症是一个重大的公共卫生问题，每年造成数百万人死亡，并给世界各地的卫生保健系统带来巨大压力。早期癌症检测至关重要，因为它能极大地提高成功治疗的机会。因此，生物标志物研究和诊断技术的进步对于创造更有效的检测策略，减少癌症对患者和医疗保健系统的影响至关重要。为了使生物标记物在临床实践中有所帮助，他们必须彻底评估其分析和临床有效性和实用性。分析效度侧重于生物标志物测定的技术准确性，包括样品处理、测定方法和结果可靠性等方面。近年来，机器学习（ML）和深度学习（DL）已成为医疗保健工程中识别生物标志物的重要工具。人工智能（AI）的进步已经将这些技术的应用扩展到各个医疗保健领域。这项研究强调了最新的创新和方法，包括先进的特征选择技术和ML/DL算法。这篇综述强调了机器学习技术在生物标志物发现中的变革性影响。它还涵盖了基于ml的平台的多个方面，详细介绍了它们在生物标志物发现中的应用和有效性。使用复杂的算法，这些先进的计算方法允许研究人员分析各种数据类型，从基因组序列到蛋白质组谱。这种方法有助于识别新的生物标志物，增强我们对癌症生物学的理解，并为通过个性化医疗改善患者护理铺平道路。这篇综述强调了未来研究的激动人心的机会，鼓励数据科学家、医疗保健专家和学术研究人员之间的持续创新和跨学科合作。

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引用次数: 0

Design, fabrication, and characterization of microfluidic-based interferometric porous silicon aptasensors for multiplexed sepsis biomarkers detection 多路脓毒症生物标志物检测微流控干涉多孔硅适体传感器的设计、制造和表征

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-09-17 DOI: 10.1016/j.snr.2025.100382

Shima Mohammadi , Fereshteh Rahimi , Ali Hossein Rezayan , Ali Abouei Mehrizi , Mahsa Sedighi , Mahya Mosayebzadeh

Multiplex biosensors are critical tools for disease diagnosis, as they enable the simultaneous detection of multiple biomarkers, providing a comprehensive assessment of complex conditions such as sepsis, where the involvement of multiple biomarkers and non-specific symptoms poses challenges to conventional diagnostics. This study presents the design and fabrication of a multiplexed microfluidic aptasensor using porous silicon (PSi) thin films as optical transducers in reflectometric interference Fourier transform spectroscopy (RIFTS). The platform targets three key sepsis biomarkers: tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and C-reactive protein (CRP). Detection relies on real-time monitoring of refractive index changes in the PSi layer, induced by biomarker binding to immobilized aptamers within the nanostructure. The manipulated channels design ensured uniform flow velocity across the biosensor zones, enhancing uniform conditions for testing in three microchannels. A microcontroller-programmed motorized XYZ translation stage enabled automated, precise, and simultaneous real-time monitoring at multiple reading points. The microfluidic aptasensor platform for detecting three sepsis biomarkers demonstrated signal variations below 7 % between individual and mixture modes, with not significant cross-reactivity, confirming selective and multiplex performance. This work advances the development of label-free, multiplex biosensing platforms, highlighting their potential for clinical diagnostics and broader applications in diverse fields.

多重生物传感器是疾病诊断的关键工具，因为它们能够同时检测多种生物标志物，为脓毒症等复杂疾病提供全面评估，其中多种生物标志物和非特异性症状的参与对传统诊断提出了挑战。本研究提出了一种多路微流控传感器的设计和制造，使用多孔硅（PSi）薄膜作为反射干涉傅立叶变换光谱（RIFTS）中的光学换能器。该平台针对三种关键的脓毒症生物标志物：肿瘤坏死因子-α (TNF-α)、白细胞介素-6 （IL-6）和c反应蛋白（CRP）。检测依赖于实时监测PSi层的折射率变化，这是由生物标志物与纳米结构内固定化适配体结合引起的。操纵通道设计确保了穿过生物传感器区域的均匀流速，增强了三个微通道测试的均匀条件。一个微控制器编程的机动化XYZ翻译平台可以在多个读取点实现自动化、精确和同步的实时监控。用于检测三种败血症生物标志物的微流控感应传感器平台在个体模式和混合模式之间的信号变化低于7%，没有显著的交叉反应性，证实了选择性和多重性能。这项工作促进了无标签、多重生物传感平台的发展，突出了它们在临床诊断和不同领域更广泛应用的潜力。

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引用次数: 0

A bienzymatic lactate biosensor with co-immobilization of horseradish peroxidase and lactate oxidase on sugarcane-derived three-dimensional carbon nanoballs cross-linked carbon aerogels 一种将辣根过氧化物酶和乳酸氧化酶共同固定在甘蔗基三维碳纳米球交联碳气凝胶上的双酶乳酸生物传感器

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-09-17 DOI: 10.1016/j.snr.2025.100383

Yashuang Hei , Lisi Ba , Ling Yu , Bingxiao Zheng , Sisi Wen , Nan Ma , Zhiju Zhao

In this work, a novel bienzymatic lactate biosensor was developed by co-immobilization of lactate oxidase (LOD) and horseradish peroxidase (HRP) onto the screen-printed electrode (SPE) modified by the three-dimensional (3D) carbon nanoballs cross-linked carbon aerogels (3D-CNCAs), which is derived from the biomass of sugarcane. The 3D-CNCAs, with large surface area and porous structure, play a crucial role in facilitating direct electron transfer between the electroactive center of HRP and the biosensor electrode. The experimental parameters including the amount of 3D-CNCAs suspension, the ratio of the two enzymes, the buffer pH and the operation potential were optimized. The bienzymatic lactate biosensor exhibits linear current response for lactate in the concentration ranges of 1-1690 μM, with a detection limit of 0.23 μM (S/N = 3), which is superior to the one based on monotypic enzyme of LOD. The bienzymatic lactate biosensor also exhibits a high level of selectivity, reproducibility, repeatability and long-term stability (87% of the original value after storage for 30 days), and was further applied in lactate detection in diluted real samples of lactic acid cream and commercial beverage. The outstanding analytical performance of the bienzymatic lactate biosensor demonstrates the preponderance of the bienzymatic over the one based on monotypic enzyme, and holds broad application prospects in the future.

本研究通过将乳酸氧化酶（LOD）和辣根过氧化物酶（HRP）共固定在三维（3D）碳纳米球交联碳气凝胶（3D- cncas）修饰的丝网印刷电极（SPE）上，开发了一种新型的双酶乳酸生物传感器。3D-CNCAs具有较大的表面积和多孔结构，在促进HRP电活性中心与生物传感器电极之间的直接电子转移方面起着至关重要的作用。对3D-CNCAs悬浮液的投加量、两种酶的配比、缓冲液pH和操作电位等实验参数进行了优化。双酶乳酸生物传感器对浓度范围为1 ~ 1690 μM的乳酸具有线性电流响应，检测限为0.23 μM (S/N = 3)，优于基于LOD单型酶的生物传感器。该双酶乳酸生物传感器还具有较高的选择性、再现性、重复性和长期稳定性（保存30天后为原始值的87%），并进一步应用于乳酸乳膏和商业饮料稀释后真实样品的乳酸检测。双酶乳酸生物传感器的优异分析性能表明了双酶乳酸生物传感器相对于单型酶乳酸生物传感器的优势，具有广阔的应用前景。

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