Pub Date : 2024-12-14DOI: 10.1016/j.snr.2024.100271
Priya Vijayaraghavan , Sathyadevi Palanisamy , Yen-Yun Wang , Pamela Berilyn So , Chia-Her Lin , Shey-Cherng Tzou , Shyng-Shiou F. Yuan , Yun-Ming Wang
Clinical diagnosis of diseases like cancer, requires rapid and ultrasensitive screening methods. β-galactosidase (β-gal) is a glycoside hydrolase enzyme, that is upregulated in senescent cells and primary ovarian cancer cells. It is considered a significant biomarker for cellular senescence and primary ovarian cancers. The current study demonstrates the designing of a bimetallic metal-organic framework, (Fe, Cu)-MOF-919, as an ultrasensitive electrochemical immunosensor for investigating ꞵ-galactosidase (β-gal) enzyme on screen-printed carbon electrodes (SPCE) using electrochemical impedance spectroscopy (EIS) in human oral cancer plasma samples. The sensor exhibited a linear response in a wide concentration of β-gal ranging from 10 fg/mL to 1 ng/mL with a limit of detection (LOD) of 4.79 fg/mL and a limit of quantification (LOQ) of 14.53 fg/mL. Furthermore, the sensor confirmed outstanding selectivity and sensitivity against biologically significant interfering molecules. Analyses of β-gal in human oral cancer samples also demonstrated the potential of β-gal for clinical diagnosis. The sensing approach holds substantial clinical relevance by being a promising option for designing latent biosensors.
{"title":"Senescence-associated β-galactosidase detection in human oral cancer samples using bimetallic (Fe, Cu)-MOF-919 impedimetric immunosensor","authors":"Priya Vijayaraghavan , Sathyadevi Palanisamy , Yen-Yun Wang , Pamela Berilyn So , Chia-Her Lin , Shey-Cherng Tzou , Shyng-Shiou F. Yuan , Yun-Ming Wang","doi":"10.1016/j.snr.2024.100271","DOIUrl":"10.1016/j.snr.2024.100271","url":null,"abstract":"<div><div>Clinical diagnosis of diseases like cancer, requires rapid and ultrasensitive screening methods. β-galactosidase (β-gal) is a glycoside hydrolase enzyme, that is upregulated in senescent cells and primary ovarian cancer cells. It is considered a significant biomarker for cellular senescence and primary ovarian cancers. The current study demonstrates the designing of a bimetallic metal-organic framework, (Fe, Cu)-MOF-919, as an ultrasensitive electrochemical immunosensor for investigating ꞵ-galactosidase (β-gal) enzyme on screen-printed carbon electrodes (SPCE) using electrochemical impedance spectroscopy (EIS) in human oral cancer plasma samples. The sensor exhibited a linear response in a wide concentration of β-gal ranging from 10 fg/mL to 1 ng/mL with a limit of detection (LOD) of 4.79 fg/mL and a limit of quantification (LOQ) of 14.53 fg/mL. Furthermore, the sensor confirmed outstanding selectivity and sensitivity against biologically significant interfering molecules. Analyses of β-gal in human oral cancer samples also demonstrated the potential of β-gal for clinical diagnosis. The sensing approach holds substantial clinical relevance by being a promising option for designing latent biosensors.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100271"},"PeriodicalIF":6.5,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-09DOI: 10.1016/j.snr.2024.100270
Saba Mahboubi , Mehdi Evazalipour , Ehsan Zamani , Nozhat Zebardast , Alireza Farokhrouz , Rahim Nosrati
Flap endonuclease 1 (FEN1) is a structure-specific nuclease that plays a role in DNA replication and genome stability. FEN1 is overexpressed in proliferative cancers and correlates with the grade and aggressiveness of the cancer, suggesting that it could potentially be considered a promising biomarker for various cancer types. Due to the initiation of cancer is related to the lack of the activity of FEN1 nuclease, the detection and quantification of FEN1 is significance for cancer diagnosis in early stage. There are various approaches to detect the different types of cancer biomarkers. In this context, biosensing platforms and nanotechnology-based methods have attracted much attention. To date, the sensitivity and performance of biosensors can enhance by nanomaterials/nanoparticles that have been incorporated in biosensing platforms. This review overview a brief classification and description of the advancement of biosensors and nanobiosensors for the detection and quantitation of FEN1 based on optical and electrochemical platforms.
{"title":"Flap endonuclease 1 (FEN1) biomarker as a new diagnostic target for the biosensing of cancer","authors":"Saba Mahboubi , Mehdi Evazalipour , Ehsan Zamani , Nozhat Zebardast , Alireza Farokhrouz , Rahim Nosrati","doi":"10.1016/j.snr.2024.100270","DOIUrl":"10.1016/j.snr.2024.100270","url":null,"abstract":"<div><div>Flap endonuclease 1 (FEN1) is a structure-specific nuclease that plays a role in DNA replication and genome stability. FEN1 is overexpressed in proliferative cancers and correlates with the grade and aggressiveness of the cancer, suggesting that it could potentially be considered a promising biomarker for various cancer types. Due to the initiation of cancer is related to the lack of the activity of FEN1 nuclease, the detection and quantification of FEN1 is significance for cancer diagnosis in early stage. There are various approaches to detect the different types of cancer biomarkers. In this context, biosensing platforms and nanotechnology-based methods have attracted much attention. To date, the sensitivity and performance of biosensors can enhance by nanomaterials/nanoparticles that have been incorporated in biosensing platforms. This review overview a brief classification and description of the advancement of biosensors and nanobiosensors for the detection and quantitation of FEN1 based on optical and electrochemical platforms.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100270"},"PeriodicalIF":6.5,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-07DOI: 10.1016/j.snr.2024.100266
Haeyeon Lee , Su Yong Go , Chae Yun Kim , Eun Joong Kim , Chung Mu Kang , Taek Dong Chung
Indium tin oxide (ITO) is a widely used electrode material in electrochemical biosensors owing to its beneficial characteristics such as transparency and chemical inertness. The exceptionally sluggish kinetics of heterogeneous electron transfer in ITO provides an exceptionally low background current, making electron mediators essential for electrochemical sensors. However, the chemical modification of ITO with molecular mediators is still challenging. Few straightforward and credible methods are available to obtain sufficiently thin mediator films firmly anchored on the ITO without losing its inherent benefits, including low background current and transparency. This study demonstrated the anodic electrografting of three osmium complexes, which allowed the formation of molecular mediator monolayers on ITO. The densely packed monolayer of osmium complexes retained their reversible redox processes over hundreds of potential cycles. We confirmed electron mediation by the modified electrodes for 4-aminophenol oxidation, suggesting a simple protocol for developing ITO-based biosensors functionalized with molecular mediators.
{"title":"Direct anodic electrografting of Osmium complexes on Indium tin oxide","authors":"Haeyeon Lee , Su Yong Go , Chae Yun Kim , Eun Joong Kim , Chung Mu Kang , Taek Dong Chung","doi":"10.1016/j.snr.2024.100266","DOIUrl":"10.1016/j.snr.2024.100266","url":null,"abstract":"<div><div>Indium tin oxide (ITO) is a widely used electrode material in electrochemical biosensors owing to its beneficial characteristics such as transparency and chemical inertness. The exceptionally sluggish kinetics of heterogeneous electron transfer in ITO provides an exceptionally low background current, making electron mediators essential for electrochemical sensors. However, the chemical modification of ITO with molecular mediators is still challenging. Few straightforward and credible methods are available to obtain sufficiently thin mediator films firmly anchored on the ITO without losing its inherent benefits, including low background current and transparency. This study demonstrated the anodic electrografting of three osmium complexes, which allowed the formation of molecular mediator monolayers on ITO. The densely packed monolayer of osmium complexes retained their reversible redox processes over hundreds of potential cycles. We confirmed electron mediation by the modified electrodes for 4-aminophenol oxidation, suggesting a simple protocol for developing ITO-based biosensors functionalized with molecular mediators.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100266"},"PeriodicalIF":6.5,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-07DOI: 10.1016/j.snr.2024.100269
Mohsin Ali , Doowon Lee , Ibtisam Ahmad , Hee-Dong Kim
The progress of both the Internet of Things (IoT) and several technologies requires new gas sensor systems that are small, low-powered, and carefully designed. A noteworthy approach in regard to achieving this goal is to develop gas sensors that are based on memristors. These sensors are often referred to as gasistors, which are gas sensors plus memristors, and are being extensively studied due to their weightlessness, high-speed recovery, and efficient miniaturized features. The aim of this research is to present an overview of the existing simulations and experimental results on the gasistor for the benefit of future investigations. The gasistor array structure is primarily utilized in simulation results in order to offer an outline of the composite gas detection approach. The experimental results introduced the response characteristics, selectivity, response speed, and recovery speed of various gasistor materials. Different recovery methods, which included pulse and trigger, were introduced in the experimental field. The last session briefly presented the future perspectives and challenges of the gasistor.
{"title":"Recent progress in memristor-based gas sensors (Gasistor; gas sensor + memristor): Device modeling, mechanisms, performance, and prospects","authors":"Mohsin Ali , Doowon Lee , Ibtisam Ahmad , Hee-Dong Kim","doi":"10.1016/j.snr.2024.100269","DOIUrl":"10.1016/j.snr.2024.100269","url":null,"abstract":"<div><div>The progress of both the Internet of Things (IoT) and several technologies requires new gas sensor systems that are small, low-powered, and carefully designed. A noteworthy approach in regard to achieving this goal is to develop gas sensors that are based on memristors. These sensors are often referred to as gasistors, which are gas sensors plus memristors, and are being extensively studied due to their weightlessness, high-speed recovery, and efficient miniaturized features. The aim of this research is to present an overview of the existing simulations and experimental results on the gasistor for the benefit of future investigations. The gasistor array structure is primarily utilized in simulation results in order to offer an outline of the composite gas detection approach. The experimental results introduced the response characteristics, selectivity, response speed, and recovery speed of various gasistor materials. Different recovery methods, which included pulse and trigger, were introduced in the experimental field. The last session briefly presented the future perspectives and challenges of the gasistor.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100269"},"PeriodicalIF":6.5,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-06DOI: 10.1016/j.snr.2024.100268
Ijaz Gul , Muhammad Akmal Raheem , Md. Reyad-ul-Ferdous , Xi Yuan , Zhenglin Chen , Chenying lv , Minjiang Chen , Jiansong Ji , Duanpo Wu , Qiang Zhao , Chenggang Yan , Dongmei Yu
Biosensors have garnered substantial attention as an emerging toolbox for analyzing biomarkers, physiological processes, food, and environmental metrices. Rapid, sensitive, and selective detection is pivotal to the practical utility of a biosensing system. Over the last few years, strides have been made in biosensing research to develop next-generation sensing systems that meet WHO's ASSURED criteria. Shorter response times, accuracy, high sensitivity, and selectivity are immensely important parameters of a biosensor, directly impacted by the biosensing signal generation and detection modality. Herein, we critically analyze the state-of-the-art signal amplification strategies for various nucleic acid and non-nucleic acid bioanalytes to improve the analytical performance of biosensing systems. We first provide a brief overview of the key components of biosensors. Next, we provide insights on state-of-the-art signal amplification strategies by dividing them into target preamplification, target enrichment, cascade reactions, biocomponent engineering, exploring functional materials, optimizing reaction conditions, designing novel microfluidic devices, and harnessing AI, along with selected examples. We evaluate the development trends of these signal enhancement approaches and discuss pros and cons of each approach. Finally, we highlight current challenges and future considerations for this emerging interdisciplinary research area.
{"title":"State-of-the-art signal amplification strategies for nucleic acid and non-nucleic acid biosensors","authors":"Ijaz Gul , Muhammad Akmal Raheem , Md. Reyad-ul-Ferdous , Xi Yuan , Zhenglin Chen , Chenying lv , Minjiang Chen , Jiansong Ji , Duanpo Wu , Qiang Zhao , Chenggang Yan , Dongmei Yu","doi":"10.1016/j.snr.2024.100268","DOIUrl":"10.1016/j.snr.2024.100268","url":null,"abstract":"<div><div>Biosensors have garnered substantial attention as an emerging toolbox for analyzing biomarkers, physiological processes, food, and environmental metrices. Rapid, sensitive, and selective detection is pivotal to the practical utility of a biosensing system. Over the last few years, strides have been made in biosensing research to develop next-generation sensing systems that meet WHO's ASSURED criteria. Shorter response times, accuracy, high sensitivity, and selectivity are immensely important parameters of a biosensor, directly impacted by the biosensing signal generation and detection modality. Herein, we critically analyze the state-of-the-art signal amplification strategies for various nucleic acid and non-nucleic acid bioanalytes to improve the analytical performance of biosensing systems. We first provide a brief overview of the key components of biosensors. Next, we provide insights on state-of-the-art signal amplification strategies by dividing them into target preamplification, target enrichment, cascade reactions, biocomponent engineering, exploring functional materials, optimizing reaction conditions, designing novel microfluidic devices, and harnessing AI, along with selected examples. We evaluate the development trends of these signal enhancement approaches and discuss <em>pros</em> and <em>cons</em> of each approach. Finally, we highlight current challenges and future considerations for this emerging interdisciplinary research area.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100268"},"PeriodicalIF":6.5,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this research we report a contribution for the development of low-cost fiber optical biosensors fabricated by the Single Mode-Multi Mode-Single Mode configuration applied for the glucose monitoring considering clinical concentrations ranges in aqueous analytes. Designed devices are evaluated using health standard detection ranges, such as healthy, pre-diabetic, and diabetic stages operating at the visible spectral region. The sensing regions has been prepared by the etching technique in order to improve the interaction between the evanescent wave with the surrounding medium followed by functionalization of enzyme oxidase glucose via the electrostatic self-assembly using by Poly(allylamine hydrochloride) as an immobilizer matrix. The increase of bilayers number over the sensor surface permits us to demonstrate the enhancement of sensitivity and limit of detection. Experimental results permitted the glucose characterization in the range from 0.3 to 2.4 mg mL-1 obtaining a response time of 9 s and a sensitivity of 1.8 nm/(mg mL-1) allowing to detect hypoglycemia and diabetes stages according to the World Health Organization standards.
{"title":"Low-cost optical fiber multimode interference biosensor based on a glucose sensitive Glucose-Oxidase enzyme thin-film","authors":"W.E. Rodríguez-Rodríguez , A.J. Rodríguez-Rodríguez , A. Juárez-Saldivar , C.R. Zamarreño , I.R. Matias","doi":"10.1016/j.snr.2024.100267","DOIUrl":"10.1016/j.snr.2024.100267","url":null,"abstract":"<div><div>In this research we report a contribution for the development of low-cost fiber optical biosensors fabricated by the Single Mode-Multi Mode-Single Mode configuration applied for the glucose monitoring considering clinical concentrations ranges in aqueous analytes. Designed devices are evaluated using health standard detection ranges, such as healthy, pre-diabetic, and diabetic stages operating at the visible spectral region. The sensing regions has been prepared by the etching technique in order to improve the interaction between the evanescent wave with the surrounding medium followed by functionalization of enzyme oxidase glucose via the electrostatic self-assembly using by Poly(allylamine hydrochloride) as an immobilizer matrix. The increase of bilayers number over the sensor surface permits us to demonstrate the enhancement of sensitivity and limit of detection. Experimental results permitted the glucose characterization in the range from 0.3 to 2.4 mg mL-1 obtaining a response time of 9 s and a sensitivity of 1.8 nm/(mg mL-1) allowing to detect hypoglycemia and diabetes stages according to the World Health Organization standards.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100267"},"PeriodicalIF":6.5,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-02DOI: 10.1016/j.snr.2024.100261
Muhammad Fadlan Raihan , Ni Luh Wulan Septiani , Gilang Gumilar , Robeth Viktoria Manurung , Siti Nurul Aisyiyah Jenie , Ahmad Nuruddin , Brian Yuliarto
Mosquito-borne diseases continue to pose a significant threat to global health, encompassing a wide range of illnesses caused by pathogens transmitted by mosquito vectors, including viruses, bacteria, and parasites. Diseases such as malaria, dengue, chikungunya, and Zika underscore the urgent need for effective detection methods, along with appropriate preventive measures to mitigate their severity. In response, electrochemical-based biosensor technologies have emerged as a promising solution for rapid, sensitive, and specific diagnosis. This review comprehensively examines recent advances in the utilization of biorecognition elements in electrochemical biosensor systems for detecting mosquito-borne diseases. The discussion covers an overview of the biorecognition elements employed, diagnostic performance assessments, design considerations, and their potential as point-of-care (POC) biosensors. By synthesizing these developments, this review emphasizes the critical role of electrochemical biosensors in advancing global health initiatives against mosquito-borne diseases. Finally, the discussion also addresses the future prospects of POC features in diagnosing these diseases.
{"title":"Developing point-of-care diagnosis using electrochemical biosensor: Mosquito-borne disease","authors":"Muhammad Fadlan Raihan , Ni Luh Wulan Septiani , Gilang Gumilar , Robeth Viktoria Manurung , Siti Nurul Aisyiyah Jenie , Ahmad Nuruddin , Brian Yuliarto","doi":"10.1016/j.snr.2024.100261","DOIUrl":"10.1016/j.snr.2024.100261","url":null,"abstract":"<div><div>Mosquito-borne diseases continue to pose a significant threat to global health, encompassing a wide range of illnesses caused by pathogens transmitted by mosquito vectors, including viruses, bacteria, and parasites. Diseases such as malaria, dengue, chikungunya, and Zika underscore the urgent need for effective detection methods, along with appropriate preventive measures to mitigate their severity. In response, electrochemical-based biosensor technologies have emerged as a promising solution for rapid, sensitive, and specific diagnosis. This review comprehensively examines recent advances in the utilization of biorecognition elements in electrochemical biosensor systems for detecting mosquito-borne diseases. The discussion covers an overview of the biorecognition elements employed, diagnostic performance assessments, design considerations, and their potential as point-of-care (POC) biosensors. By synthesizing these developments, this review emphasizes the critical role of electrochemical biosensors in advancing global health initiatives against mosquito-borne diseases. Finally, the discussion also addresses the future prospects of POC features in diagnosing these diseases.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100261"},"PeriodicalIF":6.5,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-30DOI: 10.1016/j.snr.2024.100264
Antonio Algarín , Daniel Martín , Paula Daza , Gloria Huertas , Alberto Yúfera
This paper provides an update on the sensors and actuators involved in Stem Cells (SC) differentiation processes based on electric stimulation (STIM), including both current and future progress. These techniques are applied in a range of biological and medical protocols, including cell linage derivation, tissue engineering, cellular therapy, cancer research, and cell motility. The typical methodology of SC electric STIM endeavors to emulate biological processes by applying an electrical signal to the cell culture and evaluating the cell response. Cell metabolism is electrically sensitive, responding in some manner to a given stimulus. The precise mechanism by which this occurs is not fully understood, but it is evident that changes in ion density at the cell membrane proximity must excite the cell metabolism (receptors), thereby activating its “differentiation” in response. In order to gain a deeper insight into the cellular mechanisms involved in this process, the physical variables should be better recognized, measured, and quantified during the protocol execution. This work is contributing to the development of a compilation of proposed systems, and specifications required, to identify and better understand the local conditions within the cell environment that are responsible for the activation of the differentiation processes. It is crucial that STIM systems are optimally designed and that the cell response is correctly understood. Two features will be reviewed: the setup employed and the circuits for STIM and monitoring. The nexus between these two elements are the electrodes, and this work will therefore be devoted to the realization of integrated Micro-Electrode Arrays (MEAs), and the design problems associated with it. The focus will be on MEAs, with the same size scale as the cells, and the design issues related to integrated electrodes, under electric stimulation, voltage or current modes.
{"title":"Integrated sensors for electric stimulation of stem cells: A review on microelectrode arrays (MEAs) based systems","authors":"Antonio Algarín , Daniel Martín , Paula Daza , Gloria Huertas , Alberto Yúfera","doi":"10.1016/j.snr.2024.100264","DOIUrl":"10.1016/j.snr.2024.100264","url":null,"abstract":"<div><div>This paper provides an update on the sensors and actuators involved in Stem Cells (SC) differentiation processes based on electric stimulation (STIM), including both current and future progress. These techniques are applied in a range of biological and medical protocols, including cell linage derivation, tissue engineering, cellular therapy, cancer research, and cell motility. The typical methodology of SC electric STIM endeavors to emulate biological processes by applying an electrical signal to the cell culture and evaluating the cell response. Cell metabolism is electrically sensitive, responding in some manner to a given stimulus. The precise mechanism by which this occurs is not fully understood, but it is evident that changes in ion density at the cell membrane proximity must excite the cell metabolism (receptors), thereby activating its “differentiation” in response. In order to gain a deeper insight into the cellular mechanisms involved in this process, the physical variables should be better recognized, measured, and quantified during the protocol execution. This work is contributing to the development of a compilation of proposed systems, and specifications required, to identify and better understand the local conditions within the cell environment that are responsible for the activation of the differentiation processes. It is crucial that STIM systems are optimally designed and that the cell response is correctly understood. Two features will be reviewed: the setup employed and the circuits for STIM and monitoring. The nexus between these two elements are the electrodes, and this work will therefore be devoted to the realization of integrated Micro-Electrode Arrays (MEAs), and the design problems associated with it. The focus will be on MEAs, with the same size scale as the cells, and the design issues related to integrated electrodes, under electric stimulation, voltage or current modes.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100264"},"PeriodicalIF":6.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-29DOI: 10.1016/j.snr.2024.100262
Kurdistan Fakhraldin Azeez , Abdollah Salimi , Hamed Mohtasham
Amoxicillin (AMX) is commonly used antibiotic with a broad activity against Gram-negative and Gram-positive bacteria. Therefore, it is crucial access to an instant and real-time method for accurate and correct AMX determination. Here, we have developed a three-dimensional hydrogel modified with Europium -Metal organic frameworks (Eu-MOFs) and carbon dots (CDs) as fluorophores, which allows for ratiometric fluorescence detection of AMX. The Eu-MOFs and CDs as an AMX probe trapped in Carboxymethyl cellulose (CMC) based hydrogel. In the presence of AMX, the fluorescence intensity of CDs@Eu-MOFs, enhanced at 448 nm and decreased at 568 nm under 360 nm excitation, due to energy transfer process. Under optimal conditions, AMX determined in the linear range of 10 μM to 106.7 μM, with a detection limit of 1.17 μM in liquid state. While, in the hydrogel state, the linear range for AMX detection is from 0.3 μM to 3.07 μM, with a detection limit of 0.08 μM. So, through applying hydrogel state, assay sensitivity is increased 32-times compared with conventional using liquid state. Furthermore, the change of the fluorescence color under UV irradiation also applied for colorimetric sensing of AMX at concentration up to 106 μM with detection limit 0.63 μM using smartphone RGB color sensing software. In addition, presented assay indicates strong selectivity for AMX over other biomolecules, salts and also other antibiotics. Also, it successfully detects different quantities of AMX in water and milk samples with excellent sensitivity, precision, and reliability in both liquid and hydrogel states.
{"title":"Ratiometric fluorescence quantitation of amoxicillin based on CDs@Eu-MOFs incorporated 3D hydrogel using smartphone-assisted portable dual mode visual sensing","authors":"Kurdistan Fakhraldin Azeez , Abdollah Salimi , Hamed Mohtasham","doi":"10.1016/j.snr.2024.100262","DOIUrl":"10.1016/j.snr.2024.100262","url":null,"abstract":"<div><div>Amoxicillin (AMX) is commonly used antibiotic with a broad activity against Gram-negative and Gram-positive bacteria. Therefore, it is crucial access to an instant and real-time method for accurate and correct AMX determination. Here, we have developed a three-dimensional hydrogel modified with Europium -Metal organic frameworks (Eu-MOFs) and carbon dots (CDs) as fluorophores, which allows for ratiometric fluorescence detection of AMX. The Eu-MOFs and CDs as an AMX probe trapped in Carboxymethyl cellulose (CMC) based hydrogel. In the presence of AMX, the fluorescence intensity of CDs@Eu-MOFs, enhanced at 448 nm and decreased at 568 nm under 360 nm excitation, due to energy transfer process. Under optimal conditions, AMX determined in the linear range of 10 μM to 106.7 μM, with a detection limit of 1.17 μM in liquid state. While, in the hydrogel state, the linear range for AMX detection is from 0.3 μM to 3.07 μM, with a detection limit of 0.08 μM. So, through applying hydrogel state, assay sensitivity is increased 32-times compared with conventional using liquid state. Furthermore, the change of the fluorescence color under UV irradiation also applied for colorimetric sensing of AMX at concentration up to 106 μM with detection limit 0.63 μM using smartphone RGB color sensing software. In addition, presented assay indicates strong selectivity for AMX over other biomolecules, salts and also other antibiotics. Also, it successfully detects different quantities of AMX in water and milk samples with excellent sensitivity, precision, and reliability in both liquid and hydrogel states.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100262"},"PeriodicalIF":6.5,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-28DOI: 10.1016/j.snr.2024.100263
Juhi Jannat Mim , Md. Aminul Islam , Safiullah Khan , Fardin Khan , Md. Ahadul Islam Patwary , Md. Mostafizur Rahman Arup , Nayem Hossain
Mpox, a zoonotic disease caused by the Mpox virus (MPXV), has re-emerged as a significant public health threat, particularly following the outbreak in 2022. Early and rapid detection of MPXV is crucial for controlling viral spread and preventing severe complications, particularly in vulnerable populations. While effective, traditional diagnostic methods like Polymerase Chain Reaction and genome sequencing are often costly and require complex equipment. Nanomaterial-based biosensors offer a promising alternative due to their unique physicochemical properties, high surface area, biocompatibility, and rapid response times. This review explores various nanomaterials—such as carbon nanotubes (CNTs), graphene, quantum dots (QDs), and gold nanoparticles (AuNPs)—and their application in the development of biosensors for MPXV detection, focusing on research from 2008 to 2024. These materials enable sensitive, specific, and portable biosensors that detect MPXV in real time via electrochemical, optical, piezoelectric, and calorimetric mechanisms. Each detection method leverages the virus's interaction with nanomaterial-functionalized surfaces to generate measurable signals. The review also discusses the advantages of nanomaterial-based biosensors, including enhanced sensitivity, cost-effectiveness, and portability, alongside the current challenges and future directions in the field. Nanomaterial-based biosensors could play a vital role in public health efforts to manage Mpox outbreaks by enabling early detection in clinical and point-of-care settings.
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