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Phytochemical and Antimicrobial Activity Screening of StemExtracts of Tinospora cordifolia 堇青花茎提取物的植物化学和抗菌活性筛选
Pub Date : 2020-12-24 DOI: 10.51129/ujpah-2020-29-2(6)
Pooja Bhat Niki Nautiyal
Abstract-Tinosporacordifoliaisapopularmedicinal plant which is used in several traditional medicines to cure various diseases. The common names are Amrita and Guduchi, belonging to the family of Menispermaceae. The aim of the study was to study the stem extract of traditional medicinal plant, T. cordifolia for qualitative estimation of phytoconsituents and subsequently to determine its antibacterialactivityagainsttwotestmicroorganisms Escherichia coliand Pseudomonas aeruginosato authenticate its use in traditional medicines. Stems were air-dried and coarsely powdered samples were subjectedto Soxhlet extractionusingdiversesolvents(Hexane,chloroform, ethyl acetate and methanol and water). Freshly prepared extracts were exposed to standard phytochemical analysis for qualitative estimation of phytoconsituents. The antibacterial activity of the stem extract of T. cordifolia was determined by agar well diffusion method. Phytochemical analysis revealed the presence of several phytochemicals viz., alkaloids, flavonoids, steroids, phenol, tannins, steroid, terpenoids, saponins and sugars. The methanolic extract displayed the presence of highest number of phytochemical compounds. It may be due to the higher solubility of active components in this solvent as compared to other solvents. The results revealed that the methanolic extract exhibit the effective antibacterial activity against the tested bacterial species . The studies justify the use of T. cordifolia in traditional medicines. The investigation further proposed that the metabolites present in leaf tissue of T. cordifolia can be potential source of novel natural antibacterial and antioxidant agents and can be prospective applications in food industry as an antioxidant
摘要:天麻是一种常用的药用植物,在几种传统药物中用于治疗各种疾病。常见的名字是Amrita和Guduchi,属于menispermacae家族。本研究旨在对传统药用植物堇青花茎提取物进行定性评价,并测定其对大肠杆菌和铜绿假单胞菌的抑菌活性,以验证其在中药中的应用价值。茎干风干,粗粉末状样品用不同溶剂(己烷、氯仿、乙酸乙酯、甲醇和水)进行索氏提取。新鲜制备的提取物暴露于标准植物化学分析,以定性估计植物成分。采用琼脂孔扩散法测定了菖蒲茎提取物的抑菌活性。植物化学分析显示,植物化学物质包括生物碱、类黄酮、类固醇、酚、单宁、类固醇、萜类、皂苷和糖。甲醇提取物中植物化学成分含量最高。这可能是由于与其他溶剂相比,活性成分在这种溶剂中的溶解度更高。结果表明,甲醇提取物对被试细菌具有良好的抑菌活性。这些研究证明了在传统药物中使用堇叶霉是合理的。研究进一步表明,堇青叶组织代谢产物可作为新型天然抗菌抗氧化剂的潜在来源,在食品工业中具有广阔的应用前景
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引用次数: 0
Comparative Analysis of Gluten Content of Raw and Processed Materials 原料与加工物料面筋含量的比较分析
Pub Date : 2020-12-24 DOI: 10.51129/ujpah-2020-29-2(7)
S. Chandra
Abstract-Gluten intolerance /allergies is now a silently rising problem world-wide. Gluten is a protein composite found in cereals, wheat, rice, barley and certain oat varieties. It also causes Celiac disease which is an autoimmune disease (most severe form of gluten intolerance), resulting from glutenintoleranceandisbasedongeneticintolerance. The only therapeutic treatment for the patients with gluten allergies and celiac disease is a strict gluten free diet. Rising demand for gluten free products is a task for the bakers and manufacturers to eliminate gluten completely from their products which is technically not possible. This draws the attention to the fact that mandatory analysis in this field is required. The aim of this work is to find a way to reduce the gluten content in the available materials, and also to find the source of gluten contamination after processing of raw materials. It wasnotedthatgarlicandsugarcontainingcombination were found effective and showed decrease in gluten content and that they were better additivities in the processing process than rest of others.
摘要-麸质不耐症/过敏现在是一个无声的全球上升的问题。谷蛋白是一种蛋白质复合物,存在于谷物、小麦、大米、大麦和某些燕麦品种中。它还会导致乳糜泻,这是一种自身免疫性疾病(麸质不耐症的最严重形式),由麸质不耐症和非遗传性不耐症引起。对麸质过敏和乳糜泻患者的唯一治疗方法是严格的无麸质饮食。对无谷蛋白产品的需求不断增长,这对面包师和制造商来说是一项任务要从他们的产品中完全去除谷蛋白这在技术上是不可能的。这提请注意这一事实,即需要对这一领域进行强制性分析。本工作的目的是寻找一种降低现有材料中面筋含量的方法,并找到原料加工后面筋污染的来源。值得注意的是,大蒜和含糖的混合物被发现是有效的,可以降低面筋含量,并且在加工过程中它们是比其他添加剂更好的添加剂。
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引用次数: 0
Inhibitory activity of ImmunoboosterTea against pathogens 免疫增强茶对病原菌的抑制作用
Pub Date : 2020-06-20 DOI: 10.51129/ujpah-2020-28-1(4)
ABSTRACT-Indian medicinal plants manifest miraculous effects in curing a vast range of diseases and disorders among humans and can be better called as “elixirs of life.” Currently, there is much growing interest in the use of these medicinal plants as immunomodulators of the complex immune system.Through a number of vast researches conducted in the area, it is being explored that many of the chemicals in the form of alkaloids, flavonoids, terpenoids,polysaccharides,lactones,andglycoside products are responsible to cause alterationsintheimmunomodulatoryproperties. Keeping in mind, the tremendous potential of the medicinal plants and their derived drugs, a study is designed to evaluate theinhibitoryantibacterialactivityofaimmunoboosterteaformulationagainstdifferent bacterial strains using methanol, Hexane and water as solvents.
印度药用植物在治疗人类广泛的疾病和失调方面表现出奇迹般的效果,可以更好地称为“长生不老药”。目前,人们对这些药用植物作为复杂免疫系统的免疫调节剂越来越感兴趣。通过在该领域进行的大量研究,人们正在探索生物碱、类黄酮、萜类、多糖、内酯和糖苷产品等形式的许多化学物质会引起免疫调节特性的改变。考虑到药用植物及其衍生药物的巨大潜力,我们设计了一项研究,以甲醇、己烷和水为溶剂,评估免疫增强剂配方对不同菌株的抑制和抗菌活性。
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引用次数: 0
Phytochemical Analysis and Evaluation of Anti-inflammatory Activity of Bignonia venusta(Ker Gawl.) Miers Flower Extracts 大竹藤抗炎活性的植物化学分析与评价迈尔斯花提取物
Pub Date : 2020-06-20 DOI: 10.51129/ujpah-2020-28-1-5
Abstract-The inflammatory response involves a complex array of enzyme activation, mediator release, fluid extra vasations, cell migration, tissue breakdown and repair which are aimed at host defence and usually activated in disease condition. Currently much interest has been shown in the searching of medicinal plants with anti-inflammatory activity which may lead to the discovery of new therapeutic agent that is not only used to suppress the inflammation but also used in diverse disease conditions where the inflammation response is amplifying in the disease process. In the present study, the selectionofBignoniavenustaplantforevaluation was based on its traditional usages. Preparation of different extracts from non polar and polar solvent were prepared to study the phytochemical analysis in different extracts and antiinflammatoryactivitiesofdifferentextracts. Methanol extract was found to be the richest extract for phytoconstituents and from the comparison with the standard drug aspirin, it was observed that the concentration of 2000 μg/ml of methanol extract showed maximum activity (58.0%) at 560 nm while the other extract from petroleum ether, in comparison with standard drug aspirin shows no activity.
炎症反应包括一系列复杂的酶激活、介质释放、液体额外血管、细胞迁移、组织破坏和修复,这些都是针对宿主防御的,通常在疾病状态下激活。目前,寻找具有抗炎活性的药用植物已引起人们极大的兴趣,这可能导致发现新的治疗药物,不仅用于抑制炎症,而且用于疾病过程中炎症反应放大的各种疾病条件。在本研究中,根据其传统用途,对其进行了优选。采用非极性溶剂和极性溶剂制备不同提取物,研究不同提取物的植物化学分析和抗炎活性。甲醇提取物是提取植物成分最丰富的提取物,与标准药阿司匹林对比发现,浓度为2000 μg/ml的甲醇提取物在560 nm处活性最高(58.0%),而另一种石油醚提取物与标准药阿司匹林相比没有活性。
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引用次数: 1
Phytochemical Aspects of Indian Valerian 'Tagara' (Valeriana Wallichii) from Uttarakhand, Western Himalaya 喜马拉雅西部北阿坎德邦印度缬草“塔加拉”的植物化学特征
Pub Date : 2020-06-20 DOI: 10.51129/ujpah-2020-28-1-6
Abstract-Valeriana Wallichiiis also known as TagarafoundintemperateregionsofHimalayas. It is used as herb and well documented in Ayurvedic system as well as modern medicinal system. In the present study investigation on different phytochemical constituents screening was carried out from medicinal plant,V. Wallichii. Preliminary phytochemical screening reveals the presence of different bioactive compounds such as alkaloids, flavonoids, terpenes, saponin, tannin, phytosterol, gylcosides, steroids and phenolics. GC-MSandFTIRanalysisshowedthatimportant phytochemical compounds, bond and important functional groups are present in Valeriana wallichii. There is need to study on the comprehensive phytochemical composition and pharmacological characteristics ofV.wallichii along with analysis of the biomedical researches on this multipurpose herbaceous species in near future.
摘要/ abstract摘要:缬草(valeriana wallichichi)又称塔加拉弗地(tagarafound),分布在喜马拉雅山脉的温带地区。它被用作草药,在阿育吠陀体系和现代医学体系中都有很好的记载。本研究从药用植物紫花花中筛选不同的植物化学成分。Wallichii。初步的植物化学筛选显示存在不同的生物活性化合物,如生物碱、类黄酮、萜烯、皂素、单宁、植物甾醇、苷类、类固醇和酚类物质。gc - ms和ftir分析表明,缬草中存在重要的植物化学化合物、键和重要的官能团。有必要对其综合植物化学成分和药理特性进行研究。并对该多用途草本植物的生物医学研究进行了展望。
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引用次数: 0
Formulation and Evaluation of Anti-inflammatory Herbal gel using Sarcosteema acidum 酸肌藻抗炎草药凝胶的研制与评价
Pub Date : 2020-06-20 DOI: 10.51129/ujpah-2020-28-1-10
Abstract-Inflammation is a pathophysiological response of living tissues to injuries that leads to the local accumulation of plasmatic fluid and blood cells. Although it is a defense mechanism, the complex events and mediators involved the inflammatory reaction can induce, maintain or aggravate many diseases. Therefore, the uses of anti-inflammatory agents are helpful in the therapeutic treatment of these pathologies. In this context, medicinal plants are widely used in folk medicine of many countries to treat differentinflammatoryconditionsand,inparticular, skin inflammations. Present work was designed to formulate herbal gel using extract of Sarcosteema acidumstem which provide better efficacy and to ensure biological evaluation of prepared herbal gel for safety and efficacy.
摘要:炎症是活体组织对损伤的一种病理生理反应,可导致血浆和血细胞的局部积聚。虽然是一种防御机制,但炎症反应所涉及的复杂事件和介质可诱发、维持或加重许多疾病。因此,使用抗炎药有助于这些病理的治疗。在这种情况下,药用植物在许多国家的民间医学中被广泛用于治疗不同的炎症状况,特别是皮肤炎症。本研究旨在利用酸肌提取物制备具有较好疗效的草药凝胶,并对制备的草药凝胶进行安全性和有效性的生物学评价。
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引用次数: 0
Modulatory Effect of Paste of Dried seeds of Prunus amygdaluson Ovotoxicity Induced Postmenopausal Complications in Rat 杏仁桃干籽糊剂对大鼠绝经后并发症卵毒性的调节作用
Pub Date : 2020-06-20 DOI: 10.51129/ujpah-2020-28-1-11
ABSTRACT-Menopause is a natural process in women's reproductive life at the age of 40 to 50 years and leads to deficiency of estrogen causing various complications in women's health. Prunus amygdalusnuts are abundant source of various phytoestrogens and produces effect similar to that of estrogen and can be used in menopausal women in place of HRT. In the present study ovotoxicity and menopause wasinducedinfemaleratsby4-vinylcyclohexane diepoxide (165 mg/kg, i.p. five times a week for 15 days). After induction menopause the animals were treated with the paste of dried seeds of Prunus amygdalusat the doses of 100, 200 and 400 mg/kg, p.o. for 45 days. Blood estrogen level was checked before and after administration of VCD to confirm induction of menopause. After the treatment period of 45 days blood was collected from the animals, centrifuged and serum was separated and used for the estimation of estrogen, cholesterol,triglyceride,HDL,LDLandVLDL. Ovaries were isolated from the treated animals,keptinicecoldtrisbuffer,homogenized and centrifuged. The supernatant was used for the estimation of malondialdehyde (MDA) and antioxidant enzyme (superoxide dismutase and glutathione peroxidase). Results indicate that administration of seeds of Prunus amygdalus at different dose levels do not have anyeffectonserumestrogenlevelbutsignificantlyimprovedlipidprofileanddecreased atherogenic index in postmenopausal rats. Asignificant decrease in the MDAand increase in antioxidant enzymes level was observed in postmenopausal rats after treatment with paste of dried seeds of Prunus amygdalus. Results conclude that administration of seeds of Prunus amygdalus for a long period of time may improve the cardiovascular complications of women's health after menopause.
绝经期是40 ~ 50岁女性生殖生命的自然过程,绝经期导致雌激素缺乏,对女性健康造成各种并发症。杏仁李是多种植物雌激素的丰富来源,具有与雌激素类似的作用,可用于更年期妇女替代激素替代疗法。在本研究中,用4-乙烯基二氧化环己烷(165 mg/kg,每周5次,连续15天)诱导雌性小鼠卵毒性和绝经。诱导停经后,分别用100、200、400 mg/kg的杏仁桃干籽糊处理45 d。在服用VCD前后检测血雌激素水平,以确定是否绝经。治疗45 d后采集动物血液,离心分离血清,测定雌激素、胆固醇、甘油三酯、HDL、LDLandVLDL。从处理过的动物中分离卵巢,冷藏,匀浆,离心。上清液用于丙二醛(MDA)和抗氧化酶(超氧化物歧化酶和谷胱甘肽过氧化物酶)的测定。结果表明,不同剂量的杏仁核对绝经后大鼠血清雌激素水平没有影响,但明显改善了血脂水平,降低了动脉粥样硬化指数。杏仁桃干籽糊处理后,绝经后大鼠血清mda水平明显降低,抗氧化酶水平明显升高。结果表明,长期服用杏仁核可改善绝经后妇女心血管并发症的健康状况。
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引用次数: 0
Azadirachta Indica:Indian Neem,A Natural Killer of Microorganisms 印度印楝:印度印楝,微生物的天然杀手
Pub Date : 2020-06-20 DOI: 10.51129/ujpah-2020-28-1-1
Abstract-Medicinal Plants are always of immense vital component for pharmaceutical word. Azadirachta indica, commonly known as neem, has attracted worldwide prominence in recent years owing to its wide range of medicinal properties. Neem is a medicinal plant which is always at top from ancient world to modern world. The importance of the neem tree has been recognized by the US National Academy of Sciences, which published a report in 1992 entitled 'Neem- a tree for solving global problems'. Various parts of neem are sources for mankind to treat many severe diseases. More than 140 compounds have been isolated from different parts of neem. All parts of the neem tree- leaves, flowers, seeds, fruits, roots and bark have been used traditionally for the treatment of inflammation, infections, fever, skin diseases and dental disorders. Neem leafanditsconstituentshavebeendemonstrated to exhibit immunomodulatory, anti-inflammatory,antihyperglycaemic,antiulcer,antimalarial,antifungal,antibacterial,antiviral,antioxidant,antimutagenicandanticarcinogenicproperties. Keeping these points in view the present study is aimed to study antimicrobial activity of plant extracts prepared from leaves of neem plant against Klebsiella pneumoniae,Pseudomonasaeruginosa,Bacillus subtilis, Staphylococcus aureus and Escherichia coli. There is a significant antimicrobial activity observed by neem leave extract against these microbial strains.
药用植物一直是医药世界的重要组成部分。印楝,俗称印楝,近年来因其广泛的药用特性而吸引了全世界的关注。印度楝树是一种药用植物,从古代到现代,它一直处于世界的顶端。印度楝树的重要性已经得到了美国国家科学院的认可,并于1992年发表了一份题为“印度楝树——一棵解决全球问题的树”的报告。印楝树的不同部分是人类治疗许多严重疾病的来源。从印楝树的不同部位分离出140多种化合物。印度楝树的所有部分——叶子、花、种子、果实、根和树皮——传统上都被用于治疗炎症、感染、发烧、皮肤病和牙齿疾病。印度楝叶及其成分已被证明具有免疫调节、抗炎、抗高血糖、抗溃疡、抗疟疾、抗真菌、抗菌、抗病毒、抗氧化、抗诱变和抗癌的特性。在此基础上,研究了印楝叶提取物对肺炎克雷伯菌、铜绿假单胞菌、枯草芽孢杆菌、金黄色葡萄球菌和大肠杆菌的抑菌活性。印楝叶提取物对这些微生物具有显著的抑菌活性。
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引用次数: 0
Phenylhydrazine Induced Haematotoxicity and Its Amelioration by Ethanolic Extract of Momordica charantia 苯肼诱导的血液毒性及苦瓜乙醇提取物的改善作用
Pub Date : 2020-06-20 DOI: 10.51129/ujpah-2020-28-1-7
Abstract-It has been established that several plants serve as haemoprotective agents. The mechanism is that they bind to the oxidative radicalswhichisproducedduringthehaemolysis of red blood cells and the binding restores the actual form of these cells. This way, the degenerative effect of plants as the blood is ameliorated by plants. In our study, phenyl hydrazinecausedsignificantanaemiainCharles Foster rats which was recovered by ethanolic extract of Momordica charantia.
摘要:已有研究表明,几种植物具有血液保护剂的作用。其机制是,它们与红细胞溶血过程中产生的氧化自由基结合,这种结合恢复了这些细胞的实际形态。这样一来,植物作为血液的退化作用被植物所改善。在我们的研究中,苯肼引起了查尔斯福斯特大鼠明显的贫血,并由苦瓜乙醇提取物恢复。
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引用次数: 0
In vitro antimicrobial activity of Lemon grass oil by MIC and agar well diffusion method 用MIC法和琼脂孔扩散法研究柠檬草油的体外抑菌活性
Pub Date : 2020-06-20 DOI: 10.51129/ujpah-2020-28-1-12
Abstract-Foodpreservativesareusedtoincrease the shelf life of food and to maintain the quality for longer time. In the last scenario no herbal ayurvedic preservative had been considered with respect to the use of chemical preservative. Due to increasing demands for naturalandpreservativefreecompoundspromoted an idea of the replacement of synthetic preservatives with essential oils of antimicrobial properties.Essentialoilsfrommedicinalplantsarepotentialsourceofnovelantimicrobialcompounds especially against food spoilage pathogens. The aim of this project was to study antimicrobial activity of essential oil from Lemongrass (Cymbopogan citratus)oil against food spoilage organisms Escherichia coli, Micrococcus luteus, Staphylococcus aureus andBacillus cereus and fungus Aspergillus brasilliensis, Candida albicans, Chaetomium globosum and Penicillium funiculosumusing agar well diffusion method. The antimicrobial activity was evaluated by measuring the zone of inhibition. The oil at30% concentration completely/partially inhibited the growth of foodspoilagepathogens.Thesuccessfuleffectiveness of Lemon grass oil could also play amajorroleinreplacingthechemicalpreservative.
摘要:食品防腐剂用于延长食品的保质期,延长食品的保质期。在最后一种情况下,在使用化学防腐剂方面没有考虑到草药阿育吠陀防腐剂。由于对天然和不含防腐剂的化合物的需求不断增加,人们提出了用具有抗菌特性的精油代替合成防腐剂的想法。药用植物的精油是新型抗菌化合物的潜在来源,特别是对食物腐败病原体。本课题采用琼脂孔扩散法研究柠檬草精油对食品腐败菌大肠杆菌、黄体微球菌、金黄色葡萄球菌、蜡样芽孢杆菌和真菌巴西曲霉、白色念珠菌、球毛菌和真菌青霉的抑菌活性。通过测定抑菌区来评价其抑菌活性。30%浓度的油脂完全/部分抑制了食品腐败致病菌的生长。柠檬草精油的成功功效也可以在替代化学防腐剂方面发挥重要作用。
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引用次数: 2
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Universities' Journal of Phytochemistry and Ayurvedic Heights
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