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Modulatory Effect of Whole Flour and Hydroalcoholic Extract of Finger Millet (Elusine coracana) on the Abnormalities Associated with Metabolic Syndrome in Hyperlipidemic Diabetic Rats 全粉和小谷水醇提取物对高脂血症糖尿病大鼠代谢综合征相关异常的调节作用
Pub Date : 2021-06-26 DOI: 10.51129/ujpah-june2021-30-1(6)
U. Singh
Abstract- The present study was designed to evaluate the effect of whole flour and hydroalcoholic extract of finger millet (Elusine coracana) in high fat diet (HFD) and streptozotocin induced metabolic syndrome in rats. The HFD was fed to the rats for a period of 45 days to induce hyperlipidemia. Diabetes was induced by single intraperitoneal injection of streptozotocin (65mg/kg i.p) in 0.1M citrate buffer pH 4.5. Animals with fasting blood sugar level of 250 mg/dl were considered as hyperlipidemic diabetic rats (HDR) and were selected for the study. The HDR were divided into five groups with six animals in each group and one group of normal animals. The HDR received whole flour and hydroalcoholic extract of Elusine coracana at a dose of 100, 200 and 400 mg/kg for a period of 21 days. Body weight, body mass index, fasting blood sugar level, lipid profile and the level of oxidative stress was measured in animals after the treatment. All treatments significantly decreased body weight, BMI, fasting blood sugar and also improved lipid profile in HDR as compared to the toxicant control. The treatments significantly reduced the level of lipid peroxidation and improved superoxide dismutase and reduced glutathione in the pancreas of HDR. Whole flour and hydroalcoholic extract of Elusine coracana at a dose of 200 and 400 mg/kg caused significant alleviation of the abnormalities of metabolic syndrome in rats. Keywords: Finger millet, High fat diet, Hyperlipidemic diabetic rats, Metabolic syndrome.
摘要:本研究旨在探讨高脂饮食(HFD)中全面粉和小谷水醇提取物(Elusine coracana)对链脲佐菌素诱导大鼠代谢综合征的影响。将HFD喂给大鼠45天以诱导高脂血症。在0.1M柠檬酸缓冲液pH 4.5中,单次腹腔注射链脲佐菌素(65mg/kg i.p)诱导糖尿病。将空腹血糖水平为250 mg/dl的动物作为高脂血症糖尿病大鼠(HDR)进行研究。将HDR分为5组,每组6只,1组为正常动物。HDR分别以100、200和400 mg/kg的剂量接受全面粉和海棘水醇提取物的治疗,为期21天。治疗后测定动物体重、体重指数、空腹血糖、血脂及氧化应激水平。与毒物控制相比,所有治疗均显著降低了体重、BMI、空腹血糖,并改善了HDR中的脂质谱。治疗显著降低了HDR胰腺的脂质过氧化水平,改善了超氧化物歧化酶和谷胱甘肽。200和400 mg/kg剂量的全粉和海棘水醇提取物对大鼠代谢综合征异常有显著缓解作用。关键词:小米,高脂饮食,高脂血症糖尿病大鼠,代谢综合征
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引用次数: 0
Antibacterial Activity of Rosmarinus officinalis and Lavandula angustifolia Essential Oils Against Selected Poultry Pathogenic Bacteria 迷迭香和薰衣草精油对禽类致病菌的抑菌活性研究
Pub Date : 2021-06-26 DOI: 10.51129/ujpah-june2021-30-1(7)
F.Moukhfi N.Chadli
Abstract- Poultry is the host of many species of bacteria and the intestine is the privileged place of their colonization and their persistence. These bacteria are the cause of several cases of food poisoning in humans through the consumption of eggs or soiled chicken meat. In addition, these bacteria develop resistance to antibiotics that are adjusted to poultry feed as growth promoters. Essential oils are considered as important secondary metabolites for plant defense by their antimicrobial and antioxidant properties. These essential oils may be considered as a source of natural antimicrobials for the conservation of poultry food. The aim of our study is to isolate and identify bacterial strains isolated from poultry and to determine the antibacterial and antioxidant activities of Rosmarinus officinalis and Lavandula angustifolia essential oils on these bacterial strains.Essential oils of Moroccan Rosmarinus officinalis and Lavandula angustifolia were extracted by hydrodistillation. The identification of their chemical composition are performed by gas chromatography-mass spectrometry. Antimicrobial activity of extracted essential oils against Staphylococcus aureus, Clostridium perfringens, Escherichia coli and Salmonella entiritidis was evaluated by aromatogram test and Microdilution in a liquid medium. The identification of strains are performed by several test: Gram staining, Kligler test, Catalase test, test Mobility and Api Gallery 20E. The results show that essential oils tested have a considerable antibacterial activity against all isolated bacterial strains. Keywords: Poultry feed, Essential oils, Bacterial strains, Antibiotic resistance, Antimicrobial activity.
家禽是许多细菌的宿主,肠道是它们定植和持久存在的特权场所。这些细菌是人类食用鸡蛋或被污染的鸡肉导致食物中毒的几个案例的原因。此外,这些细菌对抗生素产生耐药性,这些抗生素被调整为家禽饲料中的生长促进剂。精油具有抗菌和抗氧化的特性,被认为是植物防御的重要次生代谢物。这些精油可被视为保存家禽食品的天然抗菌剂来源。本研究的目的是分离鉴定禽源细菌,并测定迷迭香和薰衣草精油对这些细菌的抑菌和抗氧化活性。采用水蒸气蒸馏法提取摩洛哥迷迭香和薰衣草精油。采用气相色谱-质谱联用技术对其化学成分进行了鉴定。采用芳香图法和液体稀释法评价精油对金黄色葡萄球菌、产气荚膜梭菌、大肠杆菌和整体沙门氏菌的抑菌活性。通过革兰氏染色、克利格勒试验、过氧化氢酶试验、迁移率试验和Api Gallery 20E试验对菌株进行鉴定。结果表明,所测精油对所有分离菌株均有较强的抑菌活性。关键词:家禽饲料,精油,菌株,抗生素耐药性,抑菌活性
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引用次数: 0
In Vitro Evaluation of Sun Protection Factor of Plant Extracts By Ultraviolet Spectroscopy Method 紫外光谱法体外评价植物提取物的防晒系数
Pub Date : 2021-06-26 DOI: 10.51129/ujpah-june2021-30-1(8)
Geeta Bhandari Garima Negi
Abstract-Acute and chronic exposure to nonphysiological doses of ultraviolet radiation leads to variety of changes of skin ranging from sun-burn, erythema to skin cancer. For skin protection from deleterious effects of sunlight, sunscreen products are used in various forms having chemical and physical filters. However, synthetic sunscreen formulations are reported to cause adverse effects. Thus the present study was planned to evaluate the sun protection factor (SPF) of leaves extract of Murraya koenigii for their application as herbal sunscreens. The results suggested that the occurrence of active compounds responsible for ultraviolet absorption can be extracted for use in sunscreens preparations as better, cheaper and safe alternative to harmful chemical sunscreens. Keywords: UV radiations, SPF, Murraya koenigii
急性和慢性暴露于非生理剂量的紫外线辐射会导致皮肤的各种变化,从晒伤、红斑到皮肤癌。为了保护皮肤免受阳光的有害影响,各种形式的防晒产品都有化学和物理过滤。然而,据报道,合成防晒霜配方会造成不良影响。因此,本研究拟对柯尼沙叶提取物的防晒系数(SPF)进行评价,为其作为草药防晒霜的应用奠定基础。结果表明,可以提取出负责紫外线吸收的活性化合物用于防晒制剂,作为有害化学防晒霜的更好、更便宜和更安全的替代品。关键词:紫外线辐射;SPF;柯尼氏菌
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引用次数: 1
Investigation of the Essential Oil of Ormenis africana from Morocco: Revision of Chemical Composition and Antibacterial Activity 摩洛哥非洲奥梅挥发油的研究:化学成分和抗菌活性的修订
Pub Date : 2021-06-26 DOI: 10.51129/ujpah-june2021-30-1(5)
El Hanbali F Barrero A.F
Abstract- The essential oil composition from the aerial parts of Ormenis africana (Asteraceae), an endemic species from Morocco, has been investigated by GC/MS. A total of 31 compounds were identified, representing 77%. After fractionation by column chromatography, the main compound was isolated and its structure elucidated by NMR spectroscopy. The essential oil was dominated by oxygenated compounds with spathulenol (45.8%) followed by camphor (7.1%), -cadinol (5.9%) and -bisabolol (5.9%) as the main compounds. This oil can be classified as spathulenol-type according to its spathulenol content. In vitro the antibacterial activity of the whole essential oil against three Gram positive (Bacillus cereus, Enterococcus faecalis, Streptococcus C) bacteria and three Gram negative (Proteus vulgaris, Escherichia coli, Pseudomonas aeroginosa) bacteria, showed significant results. Keywords: Asteraceae, Ormenis africana, Essential oil, Spathulenol, Antibacterial activity.
摘要/ Abstract摘要:采用气相色谱/质谱法研究了摩洛哥特有植物Ormenis africana (Asteraceae)地上部分的挥发油成分。共鉴定出31个化合物,占77%。经柱层析分离,分离得到主要化合物,并用核磁共振谱分析了其结构。挥发油中以含氧化合物为主,以spathulenol(45.8%)次之,其次为樟脑(7.1%)、-cadinol(5.9%)和-bisabolol(5.9%)。根据其所含的spathulenol含量,可将其归类为spathulenol型。体外对3种革兰氏阳性菌(蜡样芽孢杆菌、粪肠球菌、C链球菌)和3种革兰氏阴性菌(寻常变形杆菌、大肠杆菌、气绿假单胞菌)的抑菌活性均有显著效果。关键词:Asteraceae, Ormenis africana,精油,Spathulenol,抗菌活性
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引用次数: 0
Design Development and Characterization of Mannosylated Carbon Nanotubes 甘露糖基化碳纳米管的设计、开发与表征
Pub Date : 2021-06-26 DOI: 10.51129/ujpah-june2021-30-1(11)
Sourabh Shukla
Abstract - The proposed study was aimed at developing and exploring the efficiency of novel mannosylated MWCNTs. For selective drug delivery to alveolar macrophages, Paclitaxel was selected for incorporation into mannosylated MWCNTs based on its anticancer activity. Hopefully, this delivery system could be safely administered through i.v. route. We expect that this approach will improve management of drug therapy in cancer patients by delivering the drug at controlled rate for prolonged period of time at a desired site. The drug was found to be white to offwhite, crystalline powder that was similar in physical appearance as mentioned in I.P. 1996. Melting point of Paclitaxel was near to reported value. Solubility of the drug in different solvents at ambient temperature depicted its solubility in methanol, ethanol, acetone, dimethylsulphoxide and chloroform, while insoluble in distilled and PBS (pH 7.4). Drug entrapment efficiency of Paclitaxel loaded mannosylated MWCNTs was found to be increased. Keywords: Paclitaxel, Mannosylated MWCNTs, Anticancer, Solubility
摘要-本研究旨在开发和探索新型甘露糖化MWCNTs的效率。为了向肺泡巨噬细胞选择性递送药物,根据其抗癌活性,选择紫杉醇掺入甘露糖基化MWCNTs中。希望这个给药系统可以通过静脉注射的方式安全地给药。我们期望这种方法通过在期望的部位以控制速率长时间递送药物来改善癌症患者的药物治疗管理。该药物被发现是白色到灰白色的结晶粉末,在物理外观上与I.P. 1996中提到的相似。紫杉醇熔点接近报道值。药物在常温下不同溶剂的溶解度描述了其在甲醇、乙醇、丙酮、二甲基亚砜和氯仿中的溶解度,而在蒸馏水和PBS (pH 7.4)中不溶。发现紫杉醇负载甘露糖化MWCNTs的药物包封效率增加。关键词:紫杉醇,甘露糖化MWCNTs,抗癌,溶解度
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引用次数: 0
Preliminary Phytochemical Screening of Leaves of Ocimum tenuiflorum 荆芥叶片的初步植物化学筛选
Pub Date : 2021-06-26 DOI: 10.51129/ujpah-june2021-30-1(9)
Shweta Tyagi I.P.Pandey
Abstract-The medicinal plants are useful for healing and curing of human diseases. Over 55% of all modern clinical drugs are of natural product origin. The plant, Ocimum tenuiflorum is highly used by the people of whole world specially Indians to cure various disorders because of the presence of phytochemical constituents. Ocimum tenuiflorum plant is known to possess anticancer, antispasmodic, antiviral, insecticide, antiseptic, analgesic, antiinflammatory, antimicrobial, antistress, Immunomodulatory, hypotensive and antioxidant properties. The present study reveals that various secondary metabolites such as glycosides, alkaloids, flavonoids, phenols, terpenoids, tannins, saponins, antraquenone , fats and oils and steroids are present in different leaf extract of the plant. Keywords: Ocimum tenuiflorum , secondary metabolites, clinical drug
摘要:药用植物具有治疗和治疗人类疾病的作用。超过55%的现代临床药物是天然产物。由于植物化学成分的存在,这种植物被全世界的人们,特别是印度人,高度使用来治疗各种疾病。众所周知,川芎具有抗癌、抗痉挛、抗病毒、杀虫、防腐、镇痛、抗炎、抗菌、抗应激、免疫调节、降血压和抗氧化等特性。本研究表明,黄酮类、酚类、萜类、单宁类、皂苷类、蒽醌类、油脂类、类固醇类等次生代谢产物均存在于不同叶提取物中。关键词:芦花;次生代谢物;临床用药
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引用次数: 0
Chemical Composition and Antimicrobial Activity of Essential oil from Scales of Moroccan Juniperus phoenicea 摩洛哥刺柏鳞片精油的化学成分及抑菌活性研究
Pub Date : 2021-06-26 DOI: 10.51129/ujpah-june2021-30-1(1)
Achraf Abdou, M. Dakir
Abstract-The essential oil of Juniperus phoenicea was obtained by hydrodistillation method using a Clevenger-type apparatus with a yield of 1.9 % and was analyzed by gas chromatography coupled to a mass spectrometer (GC-MS). Twelve volatile compounds were identified representing 99,85% of the total oil composition, while the α-pinene (78,31%), β-Myrcene (11,92%) and limonene (3,96%) were the major compounds. This essential oil was evaluated as an antibacterial and antifungal agent. The result showed that the oil presents a high biological activity as an antibacterial agent against the three tested strains Escherichia coli, Staphylococcus aureus and Pseudomonas aeroginos. It's also active as an antifungal agent against the Candida albicans with a zone inhibition of 28 mm. Keywords: Medicinal plant, Juniperus, phoenicea, Essential oil, Chemical composition, Antimicrobial activity.
摘要/ abstract摘要:采用Clevenger-type装置加氢蒸馏法提取杜松精油,得率为1.9%,采用气相色谱-质谱联用(GC-MS)对其进行分析。共鉴定出12种挥发物,占总挥发油成分的99.85%,其中α-蒎烯(78.31%)、β-月桂烯(11.92%)和柠檬烯(3.96%)为主要挥发物。该精油被评价为抗菌和抗真菌剂。结果表明,该精油对大肠杆菌、金黄色葡萄球菌和产气假单胞菌具有较高的抑菌活性。它也是一种抗真菌剂,对白色念珠菌具有28毫米的区域抑制作用。关键词:药用植物,杜松,凤凰,精油,化学成分,抗菌活性
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引用次数: 0
Inhibiting Effect of Moroccan Medicinal Plants on Crystallization of Oxalo-calcic Calculations in vitro 摩洛哥药用植物体外对草酸钙结晶的抑制作用
Pub Date : 2021-06-26 DOI: 10.51129/ujpah-june2021-30-1(3)
Abdelaziz ELAmrani El MostafaMtairag
Abstract- The aim of this work is to study the inhibitory effect of some Moroccan medicinal plants: parsley, nettle, oregano and corn beard on the crystallization of oxalocalcium urinary stones under experimental conditions which simu l a t e t h e u ri n a r y e n v ir o nme n t (physiological concentrations in calcium and oxalate, temperature and pH). The experimental tests were followed by the turbidimetric method using UV-Visible Model SP8-400 spectrophotometry, the response of which restores the concentration of calcium oxalate. The results showed that the potassium and magnesium ions which constitute the main elements of these plants compete with the calcium ions in order to combine with the oxalate ions. All the competitive reactions reflecting the affinities of the different ions towards each other contribute to the observed overall inhibition of the crystallization of calcium oxalate. Keywords: Crystallization, Urinary Calculus, Calcium oxalate, Inhibition, Moroccan Medicinal Plants
摘要/ Abstract摘要:本研究旨在研究摩洛哥药用植物欧芹、荨麻、牛至和玉米须对草酸钙尿路结石结晶的抑制作用,实验条件为:在不同的生理条件下(钙和草酸盐的生理浓度、温度和pH值)。实验后采用SP8-400型紫外可见分光光度法浊度法测定草酸钙浓度。结果表明,构成这些植物主要元素的钾和镁离子与钙离子竞争,以与草酸盐离子结合。所有反映不同离子相互亲和力的竞争反应都有助于观察到草酸钙结晶的整体抑制。关键词:结晶,尿结石,草酸钙,抑制作用,摩洛哥药用植物
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引用次数: 0
Phytochemical Screening of Bombaxceiba Flowers 杉木花的植物化学筛选
Pub Date : 2021-06-01 DOI: 10.51129/ujpah-june2021-30-1(4)
Niki Nautiyal d Sonika Patwal
Abstract- Traditional knowledge of medicinal plants is showing important and significant values to society. Bombaxceiba is an important medicinal plant of tropical and subtropical India. Its medicinal usage has been reported in the traditional systems of medicine such as Ayurveda, Siddha and Unani. The present study includes the detailed exploration of phytochemical properties of various extracts of Bombaxceiba in an attempt to provide a direction for further research. Flowers of Bombaxceiba were air-dried and its coarsely powdered samples were subjected to Soxhlet extraction using diverse solvents (petroleum ether, chloroform , ethanol, ethyl acetate and distilled water)). Freshly prepared extracts were exposed to standard phytochemical analysis for qualitative estimation of phytoconsituents. Phytochemical analysis r e v e a l e d t h e p r e s e n c e o f s e v e r a l phytochemicals viz., alkaloids, flavonoids, steroids, phenol, tannins, steroid, terpenoids and glycocides. The methanolic extract displ ayed the pr e s enc e of highe st phytochemical compounds. It may be due to the higher solubility of active components in this solvent as compared to other solvents. The studies justify the use of Bombaxceiba in traditional medicines. The investigation further propose that the metabolites present in leaf tissue of Bombaxceiba can be potential source of novel natural antibacterial and antioxidant agents which may be of prospective application in food industry as an antioxidant. Keywords: Phytochemical, Bombaxceiba, Antioxidant
摘要:药用植物的传统知识正在显示出重要的社会价值。bombaxeiba是热带和亚热带印度的重要药用植物。据报道,它的药用用途在传统的医学体系中,如阿育吠陀、悉达和乌纳尼。本研究对杉木各种提取物的植物化学性质进行了详细的探讨,试图为进一步的研究提供方向。用不同的溶剂(石油醚、氯仿、乙醇、乙酸乙酯和蒸馏水)对杉木花进行风干和粗粉样索氏提取。新鲜制备的提取物暴露于标准植物化学分析,以定性估计植物成分。植物化学分析是一种常用的植物化学分析方法,主要包括:生物碱、类黄酮、类固醇、酚、单宁、类固醇、萜类和糖酸酯等。甲醇提取物具有较高的植物化学成分含量。这可能是由于与其他溶剂相比,活性成分在这种溶剂中的溶解度更高。这些研究证明了在传统药物中使用bombaxeiba是合理的。该研究进一步表明,棉铃虫叶组织代谢产物可作为新型天然抗菌抗氧化剂的潜在来源,在食品工业中具有广阔的应用前景。关键词:植物化学,棉铃木,抗氧化剂
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引用次数: 0
Evaluation of Antibacterial Activity of Combined Plant Extract of Pyracantha crenulata and Zanthoxylum armatum 火棘和花椒联合提取物抑菌活性评价
Pub Date : 2020-12-24 DOI: 10.51129/ujpah-2020-29-2(5)
Semwal Amit Negi Sweta
Abstract-Medicinal plants represent an essential source of drugs and have played an important role in healthcaresystem.PyracanthacrenulataandZanthoxylum armatumhave been used as traditional medicine. The main aim of the study was to find the antibacterial activity of the selected plants against bacterialspecies:E.coliandPseudomonasaeruginosa. The solvents used for plant extraction were hexane, chloroform, ethanol and aqueous. The in vitro antimicrobial activity was performed by Agar disk diffusion method. The hexane and aqueous extracts showed moderate activity whereas theethanolicextractsshowedasignificantantibacterial activity. In the study Tetracycline was used as standard. The combined ethanolic extract of both the selected plant showed the synergistic effect on the bacterial strain tested. This leads to the conclusion that the combined effect can have possible application in the development of products as antimicrobial.Keywords: Pyracantha crenulata, Zanthoxylum armatum, Antibiotics, MIC, Antimicrobial activity
药用植物是一种重要的药物来源,在卫生保健系统中发挥着重要作用。pyracanthacrenulataaan和zanthoxylum armatum已被用作传统药物。研究了所选植物对大肠杆菌和铜绿假单胞菌的抑菌活性。植物提取的溶剂为己烷、氯仿、乙醇和水。采用琼脂盘扩散法测定其体外抑菌活性。正己烷和水提物具有中等抑菌活性,乙醇提取物具有显著的抑菌活性。本研究以四环素为标准品。所选两种植物的联合乙醇提取物对所选菌株表现出协同作用。由此得出结论,这种联合效应在抗菌产品的开发中具有潜在的应用前景。关键词:火棘,花椒,抗生素,MIC,抗菌活性
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引用次数: 0
期刊
Universities' Journal of Phytochemistry and Ayurvedic Heights
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