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Phytochemical analysis of chloroform extract of leaves of Skimmia laureola 月桂叶氯仿提取物的植物化学分析
Pub Date : 2022-12-24 DOI: 10.51129/ujpah-2022-33-2(6)
D. P. Pandey, S. Saxena
The air-dried and powdered leaves (3 kg) of Skimmia laureola were exhaustively defatted with light petroleum ether (60-800). The petroleum free mass extracted with 90% ethanol. The ethanol extract was concentrated under reduced pressure and a suspension of the residue was made with water, which was washed with diethyl ether for several times and then partitioned with CHCl3:H2O:MeOH (6:4:4) in a separatory funnel. The chloroform layer was separated out and concentrated under reduced pressure to give CHCl3 extract (15g). The chloroform extract (10g) was subjected to repeated CC over Si-gel eluted with different proportions of n-hexane- CHCl3 and CHCl3:MeOH afforded β-stosterol (1), (E)-3’-(4-Hydroxyphenyl)-2’-propenoic methyl ester (2), (E)-3’-(4-Hydroxy-3-methoxyphenyl)-2’-propenoic methyl ester (3), Identified as bergapten (4), Identification of these compounds were made by the analysis of their chemical and spectral data.
用轻石油醚(60-800)对空气干燥和粉末状的月桂叶(3 kg)进行脱脂处理。用90%乙醇提取无油质。将乙醇提取物减压浓缩,用水制成残渣悬浮液,用乙醚洗涤数次,然后用CHCl3:H2O:MeOH(6:4:4)在分离漏斗中进行分馏。将氯仿层分离,减压浓缩得到CHCl3萃取物(15g)。将氯仿提取物(10g)用不同比例的正己烷- CHCl3和CHCl3:MeOH在硅胶上重复CC洗脱,得到β-甾醇(1),(E)-3′-(4-羟基苯基)-2′-丙烯酸甲酯(2),(E)-3′-(4-羟基-3-甲氧基苯基)-2′-丙烯酸甲酯(3),鉴定为bergapten(4),通过化学和光谱分析对这些化合物进行鉴定。
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引用次数: 0
Ayurveda and its concept of dietetics (Pathya ahaar kalpana) – a potential approach to healthy gut Microbiota 阿育吠陀及其营养学概念(Pathya ahaar kalpana) -健康肠道微生物群的潜在途径
Pub Date : 2022-12-24 DOI: 10.51129/ujpah-2022-33-2(5)
D. Semwal, S. Joshi, A. Chauhan
The present article deals with the general history of Ayurveda and its concept of dietetics for healthy microbiota. Ayurveda is an ancient science of life which has been practiced in India for the past thousands of years. In addition to the treatment, it provides knowledge about healthy lifestyles through yoga and diet regimens. In Ayurveda, the essence to live a healthy life is through strengthening the tripods of life which are aahar, nidra and brahmacharya. The aahar on the diet not only provides the material needed by our body to grow and maintain its day-to- day function but also individual conditions mana. Therefore, there is a need to consider food types and food regimens according to dosha and prakriti to enrich healthy microbiota which further will enrich a healthy body and mind.
本文讨论了阿育吠陀的一般历史及其对健康微生物群的营养学概念。阿育吠陀是一门古老的生命科学,在印度已经实践了几千年。除了治疗之外,它还通过瑜伽和饮食方案提供有关健康生活方式的知识。在阿育吠陀中,健康生活的本质是通过加强生命的三脚架,即阿哈、尼德拉和婆罗门。饮食中的饮食不仅提供我们身体生长和维持其日常功能所需的物质,而且还提供个人条件管理。因此,有必要根据dosha和prakriti考虑食物类型和食物方案,以丰富健康的微生物群,从而进一步丰富健康的身心。
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引用次数: 0
Determination of carbohydrate levels in fruits by UV-Visible Spectrophotometer 紫外可见分光光度计测定水果中碳水化合物含量
Pub Date : 2022-12-24 DOI: 10.51129/ujpah-2022-33-2(4)
Sandeep Negi, R. Dhoundiyal, H. Chandra, H. Pant, Deepali Singha
Fruits are very nutrient-dense and incredibly healthy. A rapid method was developed for the quantitative estimation of carbohydrates present in the different natural green leafy vegetables by UV-Visible Spectro-photometer. A study was carried out to determine the soluble carbohydrate content of some selected fruit which include apple, banana, custard apple, guava, papaya, grapes, pineapple, orange, pomegranate, kiwi fruit, mango, strawberries. Soluble carbohydrate was determined by Anthrone method Spectrophotometry at wavelength of 750 nm1. For most people, between 40% and 60% of total calories should come from carbohydrates, preferably from complex carbohydrates (starches) and naturally occurring sugars2. This study was aimed at evaluating the soluble carbohydrate content of some selected fruits consumed locally with a view to determine whether they meet the dietary requirement of consumers. It is envisaged that the findings of the investigation would provide additional information on the nutritional status of the fruits.
水果营养丰富,非常健康。建立了一种紫外可见分光光度计快速定量测定天然绿叶蔬菜中碳水化合物含量的方法。对选定的几种水果进行了一项测定可溶性碳水化合物含量的研究,这些水果包括苹果、香蕉、蛋奶苹果、番石榴、木瓜、葡萄、菠萝、橙子、石榴、猕猴桃、芒果、草莓。用蒽酮分光光度法测定可溶性碳水化合物,波长750 nm1。对大多数人来说,总热量的40%到60%应该来自碳水化合物,最好是复合碳水化合物(淀粉)和天然存在的糖。本研究旨在评估本地食用的一些水果的可溶性碳水化合物含量,以确定它们是否符合消费者的膳食要求。预计调查结果将提供关于水果营养状况的更多资料。
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引用次数: 0
Solubility enhancement of antiprotozoal agent by solid dispersion and herbal tablet method to improve its rate of dissolution 固体分散和中药片剂法提高抗原虫剂溶解度,提高其溶出率
Pub Date : 2022-12-24 DOI: 10.51129/ujpah-2022-33-2(3)
Dr. Sourabh Jain, Ms. Ragini Bundela, Dr. Karunakar Shukla, Limhan Sachin Sadu
Drug dissolution is the rate limiting step for bioavailability of poor aqueous soluble drug that consequently affects the in vivo drug absorption. In the present study solid dispersion based drug delivery system of Atovaquone were successfully developed in the form of tablets with improved dissolution characteristic by forming solid dispersion with PEG 4000. For the Atovaquone formulation, F1 was chosen as it has % drug release about 46 % in 180 min. FTIR spectra of drug with other excipients have not shown any interaction and also selected formulation was stable after stability studies. The in-vitro dissolution studies revealed a considerable boost in dissolution rate of Solid dispersions of Atovaquone in contrast to pure drug. From FTIR spectroscopy, it was concluded that there was no well-defined chemical interaction between Atovaquone and PEG 4000 in Solid dispersions, as no important new peaks could be observed. The Atovaquone solid dispersion based tablet (F1) showed 30.33% drug release within first 20 min. and 46.45% drug release within 180 min.Thus from studies, it could be concluded that solid dispersion of poor aqueous soluble Atovaquone by solvent evaporation technique were effectively formulate during PEG-4000 and PVP-K 30 hydrophilic polymers. Thus, the statement can be given that the rate of dissolution and solubility of poor aqueous soluble Atovaquon can be appreciably improved by solid dispersion by use of water soluble carriers by solvent evaporation technique.
药物溶出度是水溶性差药物生物利用度的限速步骤,影响药物的体内吸收。本研究成功研制了基于固体分散体的阿托伐酮片剂给药体系,通过与PEG 4000形成固体分散体,改善了阿托伐酮的溶出特性。对于阿托伐醌制剂,选择F1,因为其在180 min内释放率约为46%。药物与其他赋形剂的FTIR光谱未显示任何相互作用,并且经稳定性研究选择的制剂是稳定的。体外溶出研究表明,与纯药物相比,阿托伐醌固体分散体的溶出率有相当大的提高。从FTIR光谱分析可知,固体分散体中阿托伐醌与PEG 4000之间没有明确的化学相互作用,没有观察到重要的新峰。阿托伐酮固体分散体片(F1)的前20 min释药率为30.33%,180 min释药率为46.45%。由此可见,溶剂蒸发技术可有效地在PEG-4000和PVP-K - 30亲水性聚合物中制备水溶性差的阿托伐酮固体分散体。因此,可以给出这样的陈述,即通过溶剂蒸发技术使用水溶性载体进行固体分散,可以明显提高水溶性差的阿托伐康的溶解速率和溶解度。
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引用次数: 0
Development of biochar from crofton weed & relationship between biochar properties and its applicability as a heavy metal removal activity 杂草生物炭的开发及生物炭性能与重金属脱除能力的关系
Pub Date : 2022-12-24 DOI: 10.51129/ujpah-2022-33-2(7)
V. Parcha, Amita Sati, Pankaj Bhandari
Biochar is a pyrogenic black carbon produced from thermal degradation of carbon-rich biomass (<700°C) in an oxygen-limited environment, and usually has a porous structure, a surface rich in oxygenated functional groups, strong adsorption capacity, and a certain degree of surface area and stability. Biochar has multiple uses, including agricultural applications for soil remediation and pollution control in water and soil. Biochar has several significant socioeconomic and environmental benefits such as carbon sequestration, pollutant removal, and soil improvement. Pyrolysis temperature affects biochar properties, which in turn determines its application potential. The collected Crofton weed (except for the roots) was washed, air-dried at room temperature, and crushed for passage through a 10-mesh sieve. Here, we examined the properties of Crofton weed biochar (C-BC) produced at different pyrolysis temperatures of 300°C, 400°C, 500°C, and 600°C. We measured the yield, ash content, pH, iodine sorption value (ISV), and elemental composition of C-BC. We also characterized C-BC using scanning electron microscopy (SEM), as well as its ability to remove Pb2+and Cd2+contaminantsfrom an aqueous solution. C- BC yield decreased with increasing pyrolysis temperature, whereas ash content and pH increased. ISV first increased at 300–400°C and decreased at 500–600°C. For C-BC produced at pyrolysis temperatures 300–600°C (C-BC300 to C-BC600, respectively), H, N, and O content decreased, but C, Ca, Mg, P, and K content in- creased with increasing temperature. All C-BCs had a certain number of pore structures. Increasing pyrolysis temperatures decreased the amount of -OH, -COOH, aliphatic C-H, and polar C-O on the C-BC surface. The percentage of Pb2+and Cd2+removed increased with increasing pyrolysis temperatures. Overall, for C-BC, a low pyrolysis temperature was beneficial for producing a more porous biochar and increased content of water-soluble calcium, magnesium, nitrogen, and phosphorus, whereas high pyrolysis temperatures yield biochar that had high alkalinity, aromaticity, and stability, as well as heavy metal removal activity
生物炭是富碳生物质(<700℃)在限氧环境下热降解产生的热原黑炭,通常具有多孔结构,表面富含含氧官能团,吸附能力强,具有一定的表面积和稳定性。生物炭有多种用途,包括用于土壤修复和水和土壤污染控制的农业应用。生物炭具有几个显著的社会经济和环境效益,如固碳、去除污染物和改善土壤。热解温度影响生物炭的性质,进而决定其应用潜力。收集的克罗夫顿杂草(除了根)被清洗,在室温下风干,并粉碎通过一个10目的筛子。在这里,我们研究了在300°C、400°C、500°C和600°C不同热解温度下产生的Crofton weed生物炭(C- bc)的特性。我们测量了产率、灰分含量、pH值、碘吸附值(ISV)和C-BC的元素组成。我们还使用扫描电子显微镜(SEM)表征了C-BC,以及它从水溶液中去除Pb2+和Cd2+污染物的能力。C- BC产率随热解温度升高而降低,而灰分含量和pH值升高。ISV在300-400°C时首先升高,在500-600°C时下降。300 ~ 600℃热解生成的C- bc(分别为C- bc300 ~ C- bc600), H、N、O含量随温度升高而降低,C、Ca、Mg、P、K含量随温度升高而升高。所有的c - bc都具有一定数量的孔隙结构。热解温度的升高降低了C-BC表面-OH、-COOH、脂肪族C-H和极性C-O的含量。随着热解温度的升高,Pb2+和Cd2+的去除率增加。总体而言,对于C-BC,低热解温度有利于制备多孔性更强的生物炭,水溶性钙、镁、氮、磷含量增加,而高热解温度制备的生物炭具有较高的碱度、芳香性和稳定性,以及重金属去除活性
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引用次数: 0
Algal population growth and dynamics of Asan Wetland 牙山湿地藻类种群生长与动态
Pub Date : 2022-12-24 DOI: 10.51129/ujpah-2022-33-2(2)
Amir Khan, F. Ishaq, Umar Farooq
A one year study of the phytoplankton community composition was carried out in the Asan wetland, a reservoir fed by River Yamuna in Uttarakhand India. In terms of bio-volume, phytoplankton community was generally dominated by Bacillariophyceae. Mean phytoplankton standing crops were highest in the wetland. The frequency and severity of algal blooms was increased significantly. To control their expansion, it was essential to identify the factors responsible for blooming of waters. Nutrient enrichment (mainly due to anthropogenic activities) and environmental factors (including the climate change) were considered the major catalyst for onset, proliferation and development of blooms. The phytoplankton of the Asan wetland was studied for one year with physical and chemical variables in relation to a pollution gradient. Analysis of the physical and chemical variables and phytoplankton density indicated that the wetland is experiencing heavy pressure of pollution due to anthropogenic activities. The dominant phytoplankton community mainly comprises of family, Chlorophyceae, Bacillariophyceae and Myxophyceae. Physical factors, though vital, had an indirect effect in facilitating the interaction among various available nutrients. In terms of phytoplankton density and diversity common genera observed include Chlorella, Chlaymydomonas, Spirogyra, Ulothrix, Hydrodictyon, Cladophora, Cosmarium, Chlorococcum, Oedogonium, Microspora, Desmidium, Chara, Zygenema, Syndesmus, Volvox, Ceratoneis, Amphora, Caloneis, Fragilaria, Navicula, Synedra, Diatoms, Gomphonema, Pinnularia, Melosira, Tabellaria, Denticula, Cymbella, Cyclotella, Nostoc, Anabaena, Oscillatoria, Rivularia, Coccochloris and Phormidium. Several genera were found most prominent during the study period having no seasonal impact on their abundance and variation. The spatial and temporal patterns observed in some of these dominant species were attributable to patterns in key environmental variables including temperature, flow, pH, dissolved oxygen and nutrient concentrations.
在印度北阿坎德邦的亚穆纳河水库牙山湿地进行了为期一年的浮游植物群落组成研究。从生物量上看,浮游植物群落总体上以硅藻门为主。湿地平均浮游植物直立作物最高。藻华发生的频率和严重程度显著增加。为了控制它们的扩张,必须确定导致水体开花的因素。营养物质的富集(主要是由于人为活动)和环境因素(包括气候变化)被认为是华花发生、扩散和发展的主要催化剂。对峨山湿地浮游植物进行了为期一年的研究,研究了与污染梯度有关的物理和化学变量。理化变量和浮游植物密度分析表明,湿地受到人为活动的污染压力较大。优势浮游植物群落主要有绿藻科、硅藻科和粘藻科。物理因素虽然至关重要,但在促进各种可用营养物质之间的相互作用方面起间接作用。在浮游植物的密度和多样性方面,观察到的常见属包括小球藻、Chlaymydomonas、Spirogyra、Ulothrix、Hydrodictyon、Cladophora、Cosmarium、Chlorococcum、Oedogonium、Microspora、Desmidium、Chara、Zygenema、Syndesmus、Volvox、Ceratoneis、Amphora、Caloneis、Fragilaria、Navicula、Synedra、Diatoms、Gomphonema、Pinnularia、Melosira、Tabellaria、Denticula、Cymbella、Cyclotella、Nostoc、Anabaena、Oscillatoria、Rivularia、Coccochloris和Phormidium。在研究期间发现了几个最突出的属,它们的丰度和变异没有季节影响。部分优势种的时空格局可归因于温度、流量、pH、溶解氧和养分浓度等关键环境变量的格局。
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引用次数: 0
Effects of Sucrose and Myo-inositol on In Vitro Shoot Multiplicationof Promising Interspecific F1 Hybrid of Eucalyptus (Eucalyptus Tereticornis X Eucalyptus Grandis) 蔗糖和肌醇对桉树苗种间杂交F1苗离体增殖的影响
Pub Date : 2022-06-18 DOI: 10.51129/ujpah-2022-32-1(2)
Barkha Kamal, Sujata Gupta, Meet Bani, KaurI D. Arya, Anshuman Mishra
Abstract –Micropropagation technique is standardized for its multiplication, using nodal segments of 25-30 years old trees i.e. promising interspecific F1 hybrid of Eucalyptus (Eucalyptus tereticornis X Eucalyptus grandis). 0.1% Mercuric chloride solution for 10-15 minutes used for surface sterilization of nodal segments followed by 0.1% fungicide treatment for 1 minute and then washed 4-5 times with sterilized distilled water. These surface sterilized nodal segments were cultured on MS medium combination with auxin and cytokinin (NAA + BAP) for axillary bud proliferation. MS medium with combin- ation of 1.5mg/l BAP + 0.1mg/l NAA gave optimum rate of axillary bud induction. The in vitro shoot were cultured on MS medium with different concentration of BAP (0.1–3.0 mg/l) alone or in combination with NAA (0.1-1.5mg/l) and supplemented with sucrose at 3% level was the best for the growth and development of shoots. These proliferated axillary shoots were excised and subcultured on MS + 1.0 mg/l BAP + 0.1mg/l NAA medium to proliferate in vitro shoots.
摘要/ Abstract摘要:利用25-30年树龄的桉树(Eucalyptus tereticornis X Eucalyptus grandis) F1种间杂交品种的节段进行繁殖,实现了微繁技术的标准化。0.1%氯化汞溶液10-15分钟用于节点段表面灭菌,再用0.1%杀菌剂处理1分钟,然后用灭菌蒸馏水洗涤4-5次。这些表面灭菌的节段在含有生长素和细胞分裂素(NAA + BAP)的MS培养基上培养,用于腋芽增殖。以1.5mg/l BAP + 0.1mg/l NAA组合的MS培养基腋芽诱导率最佳。在MS培养基上分别添加不同浓度的BAP (0.1 ~ 3.0 mg/l)和NAA (0.1 ~ 1.5mg/l),并添加3%水平的蔗糖,对离体苗的生长发育最有利。将这些增生的腋芽切除,在MS + 1.0 mg/l BAP + 0.1mg/l NAA培养基上传代培养,使离体芽增殖。
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引用次数: 0
Pharmacognostic Evaluation and Antimicrobial Activity of Some Medicinal Plants Extracts Commonly Used in Indian Traditional Medicine 印度传统医学中常用药用植物提取物的生药学评价及抗菌活性研究
Pub Date : 2022-06-18 DOI: 10.51129/ujpah-2022-32-1(9)
Mirza Azim Beg, Ragib Ali
Indian traditional medicines have been used to boost health since the time of immemorial and the achievement of contemporary medical science mainly depends on drugs initially obtained from natural resources. In the past, a large number of antimicrobial compounds were discovered from synthetic and natural products for the treatment and control of infectious agents. Adhatodavasica, Tinosporacordifolia, Glycyrrhizaglabra, Boerhaviadiffusa, and Eclipta Alba are ethnomedicinal plant. They are used in different diseases like breathing disorders, burning sensation, Cough, decrease in bone tissue, blood disorders, tuberculosis, as refrigerant, aphrodisiac, in insect bites, rheumatism, as tonic and in general debility. They are vital component of many Ayurvedic formulations. Despite the common utilization of these plants, convincing study required for reporting the pharmacognostic evaluation along with their antimicrobial activity.
印度传统药物自古以来就被用来促进健康,当代医学的成就主要依赖于最初从自然资源中获得的药物。过去,从合成和天然产物中发现了大量用于治疗和控制感染性病原体的抗菌化合物。Adhatodavasica、Tinosporacordifolia、Glycyrrhizaglabra、Boerhaviadiffusa和Eclipta Alba是民族药用植物。它们用于不同的疾病,如呼吸障碍、烧灼感、咳嗽、骨组织减少、血液紊乱、肺结核,作为制冷剂、壮阳药,用于昆虫叮咬、风湿病,作为补品和一般虚弱。它们是许多阿育吠陀配方的重要组成部分。尽管这些植物被广泛利用,但还需要令人信服的研究来报道它们的生药学评价和抗菌活性。
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引用次数: 0
Phytochemical Study and Antioxidant Activities of the Coastal Asteraceae Achillea Maritima 海岸菊科水仙的植物化学研究及抗氧化活性研究
Pub Date : 2022-06-18 DOI: 10.51129/ujpah-2022-32-1(1)
*Noureddine Mazoir
Abstract – This work was focused on the phytochemical study and antioxidant activities of hexane, dichloromethane, ethyl acetate, butanol and aqueous extracts, from the aerial parts of Achillea maritima. The antioxidant activity of Achillea maritima extracts was evaluated using the two methods DPPH and FIC. The DPPH activity revealed that ethyl acetate and butanol extracts have an important antioxidant activity at 0.12 and 0.06 mg/mL for the aerial parts of the plant with a percentage of inhibition ranging from 88.29 to 89.72 %, respectively. The results obtained showed that the ethyl acetate extract exhibited a significantly higher ferrous ion chelation activity (83.1%) than the other extracts compared to the EDTA positive control (100%). Dichloromethane and it aqueous extract showed an average capacity for chelation of Fe2+ (48.88 % and 45.81 %, respectively). However, the hexane and butanol extracts have a low chelating power (9.5% and 11.3%, respectively).
摘要/ Abstract摘要:本文主要研究了水仙(Achillea marima)地上部分的己烷、二氯甲烷、乙酸乙酯、丁醇和水提物的植物化学和抗氧化活性。采用DPPH和FIC两种方法评价水仙提取物的抗氧化活性。DPPH活性表明,乙酸乙酯和丁醇提取物在0.12和0.06 mg/mL浓度下对植物地上部位具有重要的抗氧化活性,抑制率分别为88.29% ~ 89.72%。结果表明,与EDTA阳性对照(100%)相比,乙酸乙酯提取物对亚铁离子的螯合活性(83.1%)显著高于其他提取物。二氯甲烷及其水溶液对Fe2+的螯合能力平均(分别为48.88%和45.81%)。而正己烷和丁醇提取物的螯合能力较低,分别为9.5%和11.3%。
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引用次数: 0
Screening of Antibacterial Potential and Phytochemical Analysis of Medicinal Plant Barleria Prionitis 药用植物朊芽孢杆菌抑菌潜力筛选及植物化学分析
Pub Date : 2022-06-18 DOI: 10.51129/ujpah-2022-32-1(7)
S. Tyagi, Sanjeev Kumar, Ashish S. Kumar
Medicinal and healing proper- ties of herbs are closely related to their chemical components which are classified into some major groups like alkaloids, phenols, terpenoids, steroids, saponins, tannins etc. and getting these chemicals out into the herbal remedy depends upon the solubility of these compounds in various solvents. In the present study aqueous ethanol diethyl ether acetone and methanol extracts of Barleria prionitis leaves were investigated for phyto-chemical and anti-microbial activity. The micro-organisms employed were Staphylococcus aureus Escherichia coli,and Pseudomonas, aeruginosa and Bacillus subtilis. The susceptibility of bacterial strains against the all extracts was determined using the disk diffusion method. The findings showed that potential antibacterial properties of the extracts against the organisms tested. The most susceptible microorganisms were S. aureus, while the least susceptible was E. coli. Aqueous extracts had no activity against the test bacteria the leaves of plant were found abundant with biologically active phytochemicals.
草药的药用和治疗功能与它们的化学成分密切相关,这些化学成分被分为几大类,如生物碱、酚类、萜类、类固醇、皂苷、单宁等,将这些化学物质提取到草药中取决于这些化合物在各种溶剂中的溶解度。本文研究了朊芽孢杆菌叶的乙醇、乙醚、丙酮和甲醇提取物的植物化学活性和抑菌活性。所采用的微生物有金黄色葡萄球菌、大肠杆菌、假单胞菌、铜绿杆菌和枯草芽孢杆菌。采用纸片扩散法测定菌株对各提取物的敏感性。研究结果表明,提取物对测试的微生物具有潜在的抗菌特性。最敏感的是金黄色葡萄球菌,最不敏感的是大肠杆菌。水提液对试验细菌无活性,而植物叶片中含有丰富的生物活性化学物质。
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引用次数: 0
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