Pub Date : 2023-06-24DOI: 10.51129/ujpah-2022-34-1(5)
V. Parcha, Pankaj Bhandari
Essential oils are important plant products that often have a complicated composition made up of the volatile plant components. Numerous biological actions are reportedly connected to essential oils, and a new class of aromaceuticals is being developed. The market for essential oils has increased since they are used to cure these types of ailments as a result of the emergence of various mental issues due to changing living standards, such as depression, anxiety, sleeplessness, and stress. Studies on animal models have shown that the mode of action of essential oils, which cause physiological changes in the brain, involves a number of neurotransmitter systems. Despite mounting proof that essential oils have quantifiable effects on animal behavior. Therefore the current study is proposed to investigate the physical and chemical characteristics of the essential oil obtained from the rhizome of Cyperus scariosus and assess its anxiolytic effects on the central nervous system in rodent models. Hydro distillation was used to extract the essential oil from the rhizomes of the plant. Total yield, physical properties like viscosity, specific gravity, refractive index, acidity, saponification, iodine no, etc were examined .Further to determine its impact on the central nervous system in a rodent model studies were performed at a dose level of 250 mg/kg body weight and compared to the benchmark medication caffeine @100 mg/kg body weight. The essential oil has a sustainably stimulating impact on the central nervous system in a proportion of 88% compared to caffeine's 68%, according to CNS locomotors count and physicochemical features that were in good accordance with literature value.
{"title":"Cyperus scariosus rhizome essential oil - physio-chemical characteristics and anxiolytic potential","authors":"V. Parcha, Pankaj Bhandari","doi":"10.51129/ujpah-2022-34-1(5)","DOIUrl":"https://doi.org/10.51129/ujpah-2022-34-1(5)","url":null,"abstract":"Essential oils are important plant products that often have a complicated composition made up of the volatile plant components. Numerous biological actions are reportedly connected to essential oils, and a new class of aromaceuticals is being developed. The market for essential oils has increased since they are used to cure these types of ailments as a result of the emergence of various mental issues due to changing living standards, such as depression, anxiety, sleeplessness, and stress. Studies on animal models have shown that the mode of action of essential oils, which cause physiological changes in the brain, involves a number of neurotransmitter systems. Despite mounting proof that essential oils have quantifiable effects on animal behavior. Therefore the current study is proposed to investigate the physical and chemical characteristics of the essential oil obtained from the rhizome of Cyperus scariosus and assess its anxiolytic effects on the central nervous system in rodent models. Hydro distillation was used to extract the essential oil from the rhizomes of the plant. Total yield, physical properties like viscosity, specific gravity, refractive index, acidity, saponification, iodine no, etc were examined .Further to determine its impact on the central nervous system in a rodent model studies were performed at a dose level of 250 mg/kg body weight and compared to the benchmark medication caffeine @100 mg/kg body weight. The essential oil has a sustainably stimulating impact on the central nervous system in a proportion of 88% compared to caffeine's 68%, according to CNS locomotors count and physicochemical features that were in good accordance with literature value.","PeriodicalId":432227,"journal":{"name":"Universities' Journal of Phytochemistry and Ayurvedic Heights","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128525357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-24DOI: 10.51129/ujpah-2022-34-1(1)
N. Prakash, D. Reddy, R. Sundaram, U. Babu, L. Sharath, I. Bindu, Chennu Surendra
A sensitive method coupling high-performance liquid chromatography (HPLC) with diode –array detector (DAD) and electrospray ionization mass spectrometry (MS) was optimized for separation, identification, and quantification of cinnamic acid derivatives in Cichorium intybus seed and its extract. Cinnamic acid derivatives such as chlorogenic acid, caffeic acid and Chicoric acid were quantified using respective standards. Apart from 4-o-Caffeoylqunic acid, other cinnamic acid derivative such as 3-o-caffeoylquinic acid was also identified and quantified by UV and MS/MS spectra and calculated as total caffeoylquinic acids using 4-o-caffeoylqunic acid as standard in the seed and its extract. Other cinnamic acid derivatives such as 1,3-dicaffeoylqunic acid, 1,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 3, 4-dicaffeoylquinic acid, dicaffeoylquinic acid-1 and dicaffeoylquinic acid-2 (two unknown) were identified and quantified by UV and MS spectra and calculated as total dicaffeoylquinic acids using chlorogenic acid standard in the seed and its extract. The total cinnamic acids were quantified by calculating the sum of chlorogenic acid, caffeic acid, chicoric acid, total caffeoylquinic acids(4-o-caffeoylquinic acid and 3-o-caffeoylqunic acid) and total dicaffeoyl-quinic acids(1,3-dicaffeoylqunic acid, 1,4-dicaffeoylquinic acid, 3,5-dicaffeoyl-quinic acid, 3, 4-dicaffeoylquinic acid, dicaffeoylquinic acid-1 and dicaffeoyl-quinic acid-2). The Phytochemical screening of C. intybus seed and its extract revealed that this plant is a rich source of cinnamic acid derivatives so, these markers (cinnamic acid derivatives) can used for routine quality control of Cichorium intybus seed and its extract.
{"title":"Identification and quantification of cinnamic acid derivatives in Cichorium intybus seed and its extract by High- Performance Liquid Chromatography with Diode-Array Detector (HPLC-DAD) and Electrospray Ionization Mass Spectrophotometry (LC-MS/MS)","authors":"N. Prakash, D. Reddy, R. Sundaram, U. Babu, L. Sharath, I. Bindu, Chennu Surendra","doi":"10.51129/ujpah-2022-34-1(1)","DOIUrl":"https://doi.org/10.51129/ujpah-2022-34-1(1)","url":null,"abstract":"A sensitive method coupling high-performance liquid chromatography (HPLC) with diode –array detector (DAD) and electrospray ionization mass spectrometry (MS) was optimized for separation, identification, and quantification of cinnamic acid derivatives in Cichorium intybus seed and its extract. Cinnamic acid derivatives such as chlorogenic acid, caffeic acid and Chicoric acid were quantified using respective standards. Apart from 4-o-Caffeoylqunic acid, other cinnamic acid derivative such as 3-o-caffeoylquinic acid was also identified and quantified by UV and MS/MS spectra and calculated as total caffeoylquinic acids using 4-o-caffeoylqunic acid as standard in the seed and its extract. Other cinnamic acid derivatives such as 1,3-dicaffeoylqunic acid, 1,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 3, 4-dicaffeoylquinic acid, dicaffeoylquinic acid-1 and dicaffeoylquinic acid-2 (two unknown) were identified and quantified by UV and MS spectra and calculated as total dicaffeoylquinic acids using chlorogenic acid standard in the seed and its extract. The total cinnamic acids were quantified by calculating the sum of chlorogenic acid, caffeic acid, chicoric acid, total caffeoylquinic acids(4-o-caffeoylquinic acid and 3-o-caffeoylqunic acid) and total dicaffeoyl-quinic acids(1,3-dicaffeoylqunic acid, 1,4-dicaffeoylquinic acid, 3,5-dicaffeoyl-quinic acid, 3, 4-dicaffeoylquinic acid, dicaffeoylquinic acid-1 and dicaffeoyl-quinic acid-2). The Phytochemical screening of C. intybus seed and its extract revealed that this plant is a rich source of cinnamic acid derivatives so, these markers (cinnamic acid derivatives) can used for routine quality control of Cichorium intybus seed and its extract.","PeriodicalId":432227,"journal":{"name":"Universities' Journal of Phytochemistry and Ayurvedic Heights","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125426238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-24DOI: 10.51129/ujpah-2022-34-1(4)
Naveen Kumar, Shyam Vir Singh, M. Purohit
The current study deals with the soaked wool fabrics were dyed with natural dyes extracted from the bark of Symplocos racemosa. The optimization was carried out for the different variables; concentration of dyematerial, time for extraction of dye, dyeing time, concentration of mordants methodsof mordanting. Out of the three methods of mordanting, the best shades for dye was obtained using pre-mordanting with lemon juice and vinegar andpost-mordanting with aqueous leaves extract of Rhus parviflora and aqueous leaves extract of Erythrina suberosa . The colour fastness tests indicated that all samples change in colour on exposure to light. The light coloured samples were affected more rapidly as compared to the dark ones. The Lodh bark dyed samples exhibited fairly excellent fastness to light and the results of washing fastness tests showed that dyed samples had good to excellent fastness to washing. Keywords: Symplocos racemosa (Lodh), Erythrina suberosa, Rhus parviflora, Wool fabrics, Fastness, Herbal mordants, Natural dye.
{"title":"Extraction and dyeing of eco-friendly natural dye from Lodh bark on wool fabrics and optimization of procedure for dyeing using herbal mordants","authors":"Naveen Kumar, Shyam Vir Singh, M. Purohit","doi":"10.51129/ujpah-2022-34-1(4)","DOIUrl":"https://doi.org/10.51129/ujpah-2022-34-1(4)","url":null,"abstract":"The current study deals with the soaked wool fabrics were dyed with natural dyes extracted from the bark of Symplocos racemosa. The optimization was carried out for the different variables; concentration of dyematerial, time for extraction of dye, dyeing time, concentration of mordants methodsof mordanting. Out of the three methods of mordanting, the best shades for dye was obtained using pre-mordanting with lemon juice and vinegar andpost-mordanting with aqueous leaves extract of Rhus parviflora and aqueous leaves extract of Erythrina suberosa . The colour fastness tests indicated that all samples change in colour on exposure to light. The light coloured samples were affected more rapidly as compared to the dark ones. The Lodh bark dyed samples exhibited fairly excellent fastness to light and the results of washing fastness tests showed that dyed samples had good to excellent fastness to washing. Keywords: Symplocos racemosa (Lodh), Erythrina suberosa, Rhus parviflora, Wool fabrics, Fastness, Herbal mordants, Natural dye.","PeriodicalId":432227,"journal":{"name":"Universities' Journal of Phytochemistry and Ayurvedic Heights","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122313582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-24DOI: 10.51129/ujpah-2022-34-1(7)
S. Tyagi, Ashok Kumar, A. Garg, I. P. Pandey
The aim of the present study was to investigate the presence of phytochemicals in leaves extracts (aqueous, methanol, ethanol) of Hamelia patens, the selected medicinal plant. Ethanolic and methanolic (organic) extract showed good number of compounds compared to aqueous extracts. Phyto-chemical analysis of organic extracts of Hamelia patens has shown the biological compounds like protein and amino acids, carbohydrates, cardiac glycosides, tannin, phenol, alkaloids, steroids, terpenoids, flavonoides and saponin. Quinones, phlobatannins, coumarins, emodins and fatty acids were absent in both the aqueous and organic extracts.
{"title":"Primary and secondary metabolites identification in Hamelia patens leaves","authors":"S. Tyagi, Ashok Kumar, A. Garg, I. P. Pandey","doi":"10.51129/ujpah-2022-34-1(7)","DOIUrl":"https://doi.org/10.51129/ujpah-2022-34-1(7)","url":null,"abstract":"The aim of the present study was to investigate the presence of phytochemicals in leaves extracts (aqueous, methanol, ethanol) of Hamelia patens, the selected medicinal plant. Ethanolic and methanolic (organic) extract showed good number of compounds compared to aqueous extracts. Phyto-chemical analysis of organic extracts of Hamelia patens has shown the biological compounds like protein and amino acids, carbohydrates, cardiac glycosides, tannin, phenol, alkaloids, steroids, terpenoids, flavonoides and saponin. Quinones, phlobatannins, coumarins, emodins and fatty acids were absent in both the aqueous and organic extracts.","PeriodicalId":432227,"journal":{"name":"Universities' Journal of Phytochemistry and Ayurvedic Heights","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131646294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-24DOI: 10.51129/ujpah-2022-34-1(9)
D. Raina, Manish Kumar, Banti Rana, Prachi Mall
The use of Avena sativa, also known as oat straw, in herbal lozenges for smoking cessation shows promise. This herbal ingredient has a long history of medicinal use and has demonstrated potential in reducing cravings and withdrawal symptoms related to quitting smoking. The lozenges were prepared by using Avena sativa sugar and other excipients. The physicochemical properties of the lozenges were evaluated, including weight, thickness, hardness, friability, and dissolution rate. The lozenges were also evaluated for their sensory properties, including taste, texture, and overall acceptability. 100 grams of dark chocolate was melted properly and the drug was added to it and mixed well and then added in the mold for the perfect shape and allowed to cool
{"title":"Formulation and evaluation of Chocolate Lozenges for cessation of smoking","authors":"D. Raina, Manish Kumar, Banti Rana, Prachi Mall","doi":"10.51129/ujpah-2022-34-1(9)","DOIUrl":"https://doi.org/10.51129/ujpah-2022-34-1(9)","url":null,"abstract":"The use of Avena sativa, also known as oat straw, in herbal lozenges for smoking cessation shows promise. This herbal ingredient has a long history of medicinal use and has demonstrated potential in reducing cravings and withdrawal symptoms related to quitting smoking. The lozenges were prepared by using Avena sativa sugar and other excipients. The physicochemical properties of the lozenges were evaluated, including weight, thickness, hardness, friability, and dissolution rate. The lozenges were also evaluated for their sensory properties, including taste, texture, and overall acceptability. 100 grams of dark chocolate was melted properly and the drug was added to it and mixed well and then added in the mold for the perfect shape and allowed to cool","PeriodicalId":432227,"journal":{"name":"Universities' Journal of Phytochemistry and Ayurvedic Heights","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130554854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-24DOI: 10.51129/ujpah-2022-34-1(3)
Shyam Vir Singh, Naveen Kumar, Ankita Singh, J. S. Jangwan
Medicinal Plants have been practised for hundreds of centuries by tribes all over the world. From the earliest times until the end of nineteenth century plants are still the common source of medicinal treatment yet. Using natural, plant-derived medicines that are “healthier” than prescription drugs derived from synthesized products is something that appeals to consumers. In this study, the plant Terminalia bellirica was taken for study due to the mesmerizing medicinal properties of the plant. The antibacterial activities of acetone, ethyl acetate, benzene and methanol extracts of Terminalia bellirica stem bark were tested for four pathogenic bacterial strains by agar disc diffusion method. Among the various extract, acetone extracts showed good antibacterial activity and maximum zone of inhibition was obtained for Staphylococus aureus (zone size 8mm) followed by Pseudomonas aurogenosa (10mm). The results are given in Table-1. Methanol extract showed antibacterial activity against E. coli (zone size 8mm). On the other hand ethyl acetate and benzene extract was found to be totally unaffected against these bacterial strains.
{"title":"Antibacterial activity of stem bark extract of traditionally used Terminalia bellirica (Gaerth.) Roxb.","authors":"Shyam Vir Singh, Naveen Kumar, Ankita Singh, J. S. Jangwan","doi":"10.51129/ujpah-2022-34-1(3)","DOIUrl":"https://doi.org/10.51129/ujpah-2022-34-1(3)","url":null,"abstract":"Medicinal Plants have been practised for hundreds of centuries by tribes all over the world. From the earliest times until the end of nineteenth century plants are still the common source of medicinal treatment yet. Using natural, plant-derived medicines that are “healthier” than prescription drugs derived from synthesized products is something that appeals to consumers. In this study, the plant Terminalia bellirica was taken for study due to the mesmerizing medicinal properties of the plant. The antibacterial activities of acetone, ethyl acetate, benzene and methanol extracts of Terminalia bellirica stem bark were tested for four pathogenic bacterial strains by agar disc diffusion method. Among the various extract, acetone extracts showed good antibacterial activity and maximum zone of inhibition was obtained for Staphylococus aureus (zone size 8mm) followed by Pseudomonas aurogenosa (10mm). The results are given in Table-1. Methanol extract showed antibacterial activity against E. coli (zone size 8mm). On the other hand ethyl acetate and benzene extract was found to be totally unaffected against these bacterial strains.","PeriodicalId":432227,"journal":{"name":"Universities' Journal of Phytochemistry and Ayurvedic Heights","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130486463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-24DOI: 10.51129/ujpah-2022-34-1(2)
Nikhil Singh
The use of cosmetic products has grown many folds in recent years. The consumer and regulatory bodies are more concerned about the hazardous outcome of these products on skin health. The development of alternative and effective safety assessment methodologies to avoid animal testing is an urgent need due to restrictions and ethical considerations set by various regulatory bodies such as OECD, COLIPA, and BIS. The new integrated approaches like in-vitro assays (Cell-based and Cell-free assays) are indispensable for assessing the safety of cosmetic products. In-vitro testing systems provide advantages over animal testing as they are more cost-effective, and less time-consuming. In this article, we present the details of some of the important invitro assays that are routinely used by different laboratories for validation of the safety of cosmetic ingredients.
{"title":"Cell based models for validation of safety of cosmetic products and ingredients","authors":"Nikhil Singh","doi":"10.51129/ujpah-2022-34-1(2)","DOIUrl":"https://doi.org/10.51129/ujpah-2022-34-1(2)","url":null,"abstract":"The use of cosmetic products has grown many folds in recent years. The consumer and regulatory bodies are more concerned about the hazardous outcome of these products on skin health. The development of alternative and effective safety assessment methodologies to avoid animal testing is an urgent need due to restrictions and ethical considerations set by various regulatory bodies such as OECD, COLIPA, and BIS. The new integrated approaches like in-vitro assays (Cell-based and Cell-free assays) are indispensable for assessing the safety of cosmetic products. In-vitro testing systems provide advantages over animal testing as they are more cost-effective, and less time-consuming. In this article, we present the details of some of the important invitro assays that are routinely used by different laboratories for validation of the safety of cosmetic ingredients.","PeriodicalId":432227,"journal":{"name":"Universities' Journal of Phytochemistry and Ayurvedic Heights","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127180994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-24DOI: 10.51129/ujpah-2022-34-1(6)
S. Farooq, Z. Mehmood, A. K. Dixit
Antimicrobial resistance (AMR) is a serious and growing threat to human health. The development of new antibiotics is limited and slow. The tradition of synergy in herbal medicine is being used as a source of research ideas. The in vitro findings were that most of the research reported synergy both within plants and between plants and antibiotics. Whole plant extracts and combinations of compounds were shown to be more effective antimicrobials than isolated constituents. New sources of antimicrobial drugs need to be identified and improved strategy should be developed to combat multidrug resistance problem in pathogenic bacteria. Plant extract and phytochemicals demonstrating antimicrobial action needs to be exploited for their synergistic action between extracts and with antibiotics to exploit it in modern phytomedicine and combinational therapy. In the present study alcoholic extracts of medicinal plants were screened for their antimicrobial efficacy against drug resistant bacteria. The extracts of Hemidesmus indicus,and Carum copticum, showed promising action against one or more drug resistant bacteria with MIC ranged from 0.53 mg/ml to 7.80 mg/ml which has indicated their potential to be exploited in antimicrobial therapy and combination drug therapy after careful evaluation in vivo model.
{"title":"Antimicrobial potentials of plant extracts against drug resistant bacteria","authors":"S. Farooq, Z. Mehmood, A. K. Dixit","doi":"10.51129/ujpah-2022-34-1(6)","DOIUrl":"https://doi.org/10.51129/ujpah-2022-34-1(6)","url":null,"abstract":"Antimicrobial resistance (AMR) is a serious and growing threat to human health. The development of new antibiotics is limited and slow. The tradition of synergy in herbal medicine is being used as a source of research ideas. The in vitro findings were that most of the research reported synergy both within plants and between plants and antibiotics. Whole plant extracts and combinations of compounds were shown to be more effective antimicrobials than isolated constituents. New sources of antimicrobial drugs need to be identified and improved strategy should be developed to combat multidrug resistance problem in pathogenic bacteria. Plant extract and phytochemicals demonstrating antimicrobial action needs to be exploited for their synergistic action between extracts and with antibiotics to exploit it in modern phytomedicine and combinational therapy. In the present study alcoholic extracts of medicinal plants were screened for their antimicrobial efficacy against drug resistant bacteria. The extracts of Hemidesmus indicus,and Carum copticum, showed promising action against one or more drug resistant bacteria with MIC ranged from 0.53 mg/ml to 7.80 mg/ml which has indicated their potential to be exploited in antimicrobial therapy and combination drug therapy after careful evaluation in vivo model.","PeriodicalId":432227,"journal":{"name":"Universities' Journal of Phytochemistry and Ayurvedic Heights","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127325964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-24DOI: 10.51129/ujpah-2022-33-2(1)
M. A. Abu Zaid, M. Chauhan, Swarndeep Chauhan
Withania Somnifera (Ashwagandha) is an important medicinal plant that has been used in Ayurvedic and indigenous medicine. It is also known as Indian ginseng. It exerts antioxidant, anti-inflammatory and immune modulatory activities. In the present study, we studied the free radical scavenging activity of Withania Somnifera root extract. The radical scavenging effect of the extract was studied by its effect on hydroxy radicals generated by Fe-ascorbate-H2O2 system. Our results showed that Withania Somnifera root extract (1-10,000 µg/ ml) resulted in a significant dose dependent increase in percent inhibition of hydroxylation. Further, we studied the in vitro effect of Withania Somnifera root extract on Lipid peroxidation in terms of MDA level of human erythrocytes. Our results showed a significant dose dependent decrease in lipid peroxidation at a concentration of (1-1000 µg/ ml) as evident by decrease in level of MDA. Maximum effect was observed at 100-1000 µg/ ml. Withania Somnifera root extract thus can act as a potential antioxidant containing compound and protects erythrocytes from oxidative damage by either directly scavenging the free radical, sparing it or activating other enzymatic processes. At higher concentration (10,000 µg/ ml), Withania Somnifera extract increased the oxidative stress as evident from erythrocyte MDA level which became significantly more than the maximum effect shown at 1000 µg/ ml of extract.
{"title":"Protective role of Withania somnifera root extract on lipid peroxidation of Erythrocytes","authors":"M. A. Abu Zaid, M. Chauhan, Swarndeep Chauhan","doi":"10.51129/ujpah-2022-33-2(1)","DOIUrl":"https://doi.org/10.51129/ujpah-2022-33-2(1)","url":null,"abstract":"Withania Somnifera (Ashwagandha) is an important medicinal plant that has been used in Ayurvedic and indigenous medicine. It is also known as Indian ginseng. It exerts antioxidant, anti-inflammatory and immune modulatory activities. In the present study, we studied the free radical scavenging activity of Withania Somnifera root extract. The radical scavenging effect of the extract was studied by its effect on hydroxy radicals generated by Fe-ascorbate-H2O2 system. Our results showed that Withania Somnifera root extract (1-10,000 µg/ ml) resulted in a significant dose dependent increase in percent inhibition of hydroxylation. Further, we studied the in vitro effect of Withania Somnifera root extract on Lipid peroxidation in terms of MDA level of human erythrocytes. Our results showed a significant dose dependent decrease in lipid peroxidation at a concentration of (1-1000 µg/ ml) as evident by decrease in level of MDA. Maximum effect was observed at 100-1000 µg/ ml. Withania Somnifera root extract thus can act as a potential antioxidant containing compound and protects erythrocytes from oxidative damage by either directly scavenging the free radical, sparing it or activating other enzymatic processes. At higher concentration (10,000 µg/ ml), Withania Somnifera extract increased the oxidative stress as evident from erythrocyte MDA level which became significantly more than the maximum effect shown at 1000 µg/ ml of extract.","PeriodicalId":432227,"journal":{"name":"Universities' Journal of Phytochemistry and Ayurvedic Heights","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124209740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-24DOI: 10.51129/ujpah-2022-33-2(11)
Sonakshi Chandra
Staphylococcus aureus is one of the most in famous and widespread bacterial pathogen. This pathogen can cause a wide variety of diseases, ranging from moderately severe skin infections to fatal pneumonia and sepsis. Treatment of Staph. aureus infections is complicated due to antibiotic resistance. There has been ongoing and increasing interest in the extraordinarily high number of toxins and other virulence determinants that Staph. aureus produces and how they impact disease. In the past few decades, a more dangerous form of Staph.aureus has emerged. This form is known as Methicillin-Resistant Staph.aureus and usually referred to by the acronym MRSA. Keeping in view of the problem of Antimicrobial Resistance(AMR).This study was designed to explore antibacterial activity of a common kitchen herb Murraya koengii against pathogenic gram positive Staphylococcus aureus.Four different plant extracts were prepared using solvents- Methanol, Hexane, Aqueous and Ethanol+Aqueous (1:1) and tested against Staphylococcus aureus by using Kirby bauer Disc diffusion method. Methicillin antibiotic disc was used as the positive control.
{"title":"Inhibitory activity of organic solvent extracts of Murraya koenigii on Staphylococcus aureus","authors":"Sonakshi Chandra","doi":"10.51129/ujpah-2022-33-2(11)","DOIUrl":"https://doi.org/10.51129/ujpah-2022-33-2(11)","url":null,"abstract":"Staphylococcus aureus is one of the most in famous and widespread bacterial pathogen. This pathogen can cause a wide variety of diseases, ranging from moderately severe skin infections to fatal pneumonia and sepsis. Treatment of Staph. aureus infections is complicated due to antibiotic resistance. There has been ongoing and increasing interest in the extraordinarily high number of toxins and other virulence determinants that Staph. aureus produces and how they impact disease. In the past few decades, a more dangerous form of Staph.aureus has emerged. This form is known as Methicillin-Resistant Staph.aureus and usually referred to by the acronym MRSA. Keeping in view of the problem of Antimicrobial Resistance(AMR).This study was designed to explore antibacterial activity of a common kitchen herb Murraya koengii against pathogenic gram positive Staphylococcus aureus.Four different plant extracts were prepared using solvents- Methanol, Hexane, Aqueous and Ethanol+Aqueous (1:1) and tested against Staphylococcus aureus by using Kirby bauer Disc diffusion method. Methicillin antibiotic disc was used as the positive control.","PeriodicalId":432227,"journal":{"name":"Universities' Journal of Phytochemistry and Ayurvedic Heights","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124434488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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