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Historical perspective and new avenues to control the myiasis-causing fly Cochliomyia hominivorax in Uruguay 乌拉圭蝇蛆病防治的历史展望与新途径
Pub Date : 2021-11-11 DOI: 10.31285/agro.25.974
Fresia, S. Pimentel, V. Iriarte, U. Cuore, A. Menchaca
‘ Mosca de la bichera ’ or simply ‘ bichera ’ are common names given in Uruguay and the region to the primary myiasis-causing species Cochliomyia hominivorax , the New World Screwworm (NWS) fly (Diptera: Calliphori-dae ). Myiasis happens when dipteran larvae infest live animals at least during some developmental phase to feed on host’s flesh and fluids. For the NWS fly it is mandatory that all three larvae phases develop on living tissues of warm-blooded vertebrates, including humans. Unsurprisingly, this parasitic behavior causes great profit losses to the livestock industry and is also considered a neglected public health issue. NWS is endemic from the tropics and subtropics of the Americas, but has been eradicated from North and Central America through a Sterile Insect Technique (SIT) based Area Wide – Integrated Pest Management (AW-IPM) program that lasted more than 50 years. Since 2004, a permanent barrier is actively maintained in Darien, along the Panama-Colombian border, by releasing 14 million sterile flies per week to avoid reintroductions. Due to its direct and indirect impact on the national economy, the logistic complexity and the cost of SIT control programs, much discussion is underway in Uruguay about NWS fly eradication. Direct economic losses due to myiasis in Uruguay oscillate between USD 40 and 154 million annually (i.e., between 2-8% of livestock Gross Domestic Product, GDP). Currently, the Food and Agriculture Organization of the UN/International Atomic Energy Agency (FAO/IAEA) and the US Department of Agriculture/Panama United States Commission for the Eradication and Prevention of Screwworm/ Ministry of Livestock, Agriculture and Fisheries (USDA/COPEG/MGAP) have been working on eradication proposals for Uruguay. Cost-benefit analysis of each group concluded that a net present value of around USD 98 million and USD 146 million could be achieved, respectively, supporting the positive impact of NWS fly eradication at local farmers and the whole livestock sector levels. The main challenge of this endeavor is to find a way to keep the myiasis-free status of Uruguay in case that its neighbors, Argentina and Brazil, do not engage in a similar program, at least for their southernmost region. Here we review the bulk of bibliography produced since the beginning of NWS eradication programs in North America during the 40s decade, its life cycle and parasitic lifestyle as well as many aspects of its population genetics and ecology. We further discuss promising biotechnological approaches under active development based on transgenesis and CRISPR/Cas genome-editing, that are considered the new avenue in insect-control strategies. Balance among innovation and regulation framework is considered based on lessons learned. Currently, a CRISPR/Cas gene editing strategy for gene drive is being investigated in Uruguay, a development conducted with national funds, what guarantees its complete control and local institutions, authorities and ultima
“Mosca de la bichera”或简称为“bichera”,是乌拉圭和该地区主要引起蝇蛆病的物种,即新世界螺旋蝇(NWS)蝇(双翅目:螺旋蝇科)的常见名称。蝇蛆病发生时,双翅目幼虫感染活的动物,至少在某些发育阶段,以宿主的肉和液体为食。对于NWS蝇来说,所有三个幼虫阶段都必须在温血脊椎动物(包括人类)的活组织上发育。不出所料,这种寄生行为给畜牧业造成巨大的利润损失,也被认为是一个被忽视的公共卫生问题。NWS是美洲热带和亚热带地区的地方病,但通过一项持续50多年的基于昆虫不育技术(SIT)的区域综合有害生物管理(AW-IPM)计划,已从北美和中美洲根除。自2004年以来,巴拿马-哥伦比亚边境沿线的达连积极维持了一个永久性屏障,每周释放1400万只不育苍蝇,以避免再次引入。由于其对国民经济的直接和间接影响、物流复杂性和SIT控制计划的成本,乌拉圭正在进行许多关于NWS苍蝇根除的讨论。乌拉圭每年因蝇蛆病造成的直接经济损失在4000万至1.54亿美元之间(即占畜牧业国内生产总值的2-8%)。目前,联合国粮食及农业组织/国际原子能机构(粮农组织/原子能机构)和美国农业部/巴拿马美国根除和预防螺旋蝇委员会/畜牧、农业和渔业部(USDA/COPEG/MGAP)正在为乌拉圭制定根除建议。对每一组的成本效益分析得出的结论是,净现值分别可达到约9800万美元和1.46亿美元,支持在当地农民和整个畜牧业层面消灭NWS苍蝇的积极影响。这项努力的主要挑战是找到一种方法来保持乌拉圭无蝇蛆病的地位,以防其邻国阿根廷和巴西不参与类似的计划,至少在其最南端地区。在这里,我们回顾了自40年代北美NWS根除计划开始以来产生的大量参考文献,它的生命周期和寄生生活方式以及它的种群遗传学和生态学的许多方面。我们进一步讨论了基于转基因和CRISPR/Cas基因组编辑的积极开发的有前途的生物技术方法,这些方法被认为是昆虫控制策略的新途径。在吸取经验教训的基础上考虑创新和监管框架之间的平衡。目前,乌拉圭正在研究一种用于基因驱动的CRISPR/Cas基因编辑策略,这是一项由国家基金开展的进展,确保其完全控制,并确保地方机构、当局和最终的牲畜生产者可以成为生物技术的所有者。最后,我们强调将产生的专门知识,为在当地为与兽医和公共卫生高度相关的其他寄生虫和/或病媒昆虫制定新的基于遗传的控制战略提供了可能性。
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引用次数: 2
Deficit irrigation in soybeans, the effect on grain yield and water productivity in temperate climates 温带条件下大豆亏缺灌溉对籽粒产量和水分生产力的影响
Pub Date : 2021-10-01 DOI: 10.31285/agro.25.500
R. Hayashi, S. Dogliotti
In regions with a temperate and humid climate, deficit irrigation improves the profitability and sustainability of production systems, when properly implemented. It is necessary to generate knowledge on the response of crops to deficit irrigation. Aiming to improve the design and management of irrigation equipment used in soybean cultivation in Uruguay, an experiment was proposed where three maximum depths of irrigation replacement (3 mm, 6 mm and 9 mm day-1) and non-irrigated control were evaluated. A randomized complete block design was used, with four treatments and four replications. Drip irrigation was applied, which allowed a good adjustment of the different irrigation depths used and reduced the experimental area. Non-irrigated and 3 mm suffered water stress from V10 to grain filling, producing lower yields. The 6 mm treatment yielded the same as the 9 mm in Year 1, but lower in Year 2. This study indicates that irrigation is necessary when rainfall is less than the average for the region or its distribution is inadequate. The 9 mm treatment showed the highest water productivity (WUE: 0.86 Kg m-3) and irrigation (IWUE: 0.54 Kg m-3), with less use of rainfall, compared to 6mm.
在气候温和湿润的地区,亏欠灌溉如果实施得当,可提高生产系统的盈利能力和可持续性。有必要了解作物对亏缺灌溉的反应。为了改进乌拉圭大豆种植灌溉设备的设计和管理,提出了一项试验,对3种最大灌溉深度(3 mm、6 mm和9 mm day-1)和非灌溉控制进行了评价。采用随机完全区组设计,4个处理,4个重复。采用滴灌,可以很好地调节不同灌溉深度,减少了试验面积。从V10期到灌浆期,非灌水和3mm灌浆均遭受水分胁迫,产量较低。6 mm处理的产量与第1年的9 mm处理相同,但第2年较低。这项研究表明,当降雨量低于该地区的平均降雨量或其分布不足时,灌溉是必要的。与6mm处理相比,9mm处理表现出最高的水分生产力(WUE: 0.86 Kg m-3)和灌溉(IWUE: 0.54 Kg m-3),降雨利用较少。
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引用次数: 0
Effect of sowing density on the productivity of a native grassland restored with Bromus auleticus 播种密度对灰雀稗恢复原生态草地生产力的影响
Pub Date : 2021-09-06 DOI: 10.31285/AGRO.25.333
Rodolfo Andrés Franco Aquino, M. Jaurena, R. Reyno
High stocking rate livestock management has determined the decline of native species of high forage value as Bromus auleticus Trin ex Nees in the Campos region of the Rio de la Plata grasslands. The species has some agronomic and ecological characteristics that define it as of high potential to be reintroduced in degraded natural grasslands. However, Bromus auleticus has not been sufficiently described, since commercial seeds and information regarding the establishment phase are scarce. Two field experiments were carried out in 2013 and 2014, reintroducing Bromus auleticus into native grasslands in the Basaltic region, aiming to evaluate the impact of sowing density in the forage productivity and their seasonal distribution. The treatments consisted of different sowing densities: 20, 40, 60 and 80 kg ha-1 and one control treatment. Considering the management and the evaluated period (4 years for experiments 2013, and 3 years for experiments 2014), different sowing densities did not modify the forage production in any of the plantings for the evaluated period and compared to the control. However, the use of sowing densities of 40 kg ha-1 or higher allowed modifying the seasonal distribution of forage production with a better contribution in autumn-winter, and more stable production throughout the year.
在拉普拉塔河草原坎波斯地区,高放养率的畜牧业管理导致了高牧草价值的本地物种Bromus auleticus Trin ex Nees的减少。该物种具有一定的农艺学和生态学特征,具有在退化的天然草地中重新引入的高潜力。然而,由于商业种子和关于建立阶段的信息很少,因此没有充分描述过凤梨。在2013年和2014年进行了两次野外试验,在玄武岩地区的原生草地上重新引入黄雀稗,旨在评估播种密度对牧草生产力及其季节分布的影响。各处理分别为20、40、60、80 kg hm -1和1个对照处理。考虑到管理和评估期(2013年试验4年,2014年试验3年),与对照相比,不同的播种密度在评估期没有改变任何种植的饲料产量。然而,使用40 kg ha-1或更高的播种密度可以改变牧草生产的季节分布,秋冬季的贡献更好,全年的产量更稳定。
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引用次数: 0
Nitrogen fertilization strategies for center-pivot irrigated maize crop 中心-枢纽灌溉玉米作物氮肥施肥策略研究
Pub Date : 2021-08-26 DOI: 10.31285/agro.25.412
Juan Manuel Ernst, A. Beretta, Mónica M. Barbazán, L. Puppo
In the last 15 years, there has been an expansion of the use of center-pivots for irrigation in Uruguay. This equipment allows the application of diluted fertilizer in the irrigation water. The study aimed to generate information on fertilization strategies with nitrogen (N) in irrigated maize crops. A study was performed where five treatments were evaluated: T0, without additional nitrogen fertilization to the basal sowing fertilization; C0, cover fertilization with 150 Kg N ha-1 split in three (V3) and seven leaves (V7); F1, idem “C0” but with liquid fertilizer in water; F2, 150 Kg N ha-1 as liquid fertilizer in water split weekly from V3 to reproductive stage (R1); F3, idem "F2" but with 225 Kg N ha-1. Irrigation was done according to water balance and the soil moisture was measured with an FDR probe. The N plant level and biomass were evaluated in states V3, V6, R1 and physiological maturity (R6). The grain yield was determined at harvest. The correlation between the color values and Color Index (InCol) was analyzed in R1 using aerial photography and software, with: % N; biomass; Nitrogen Sufficiency Index (NSI); and grain yield (kg ha-1). The yield was higher in the treatment with the highest N dose, with no significant differences. No differences were found between the sources of N (sulfur urea and Fertec®) for the evaluated variables. A high correlation was obtained between the colors and the InCol and the cultivation variables.
在过去的15年里,乌拉圭扩大了对中心支点灌溉的使用。这种设备可以在灌溉水中施用稀释的肥料。本研究旨在为玉米灌溉作物氮素施肥策略提供信息。进行了一项研究,其中评估了五种处理:T0,在基础播种施肥的基础上不额外施肥;C0,覆盖施肥150 Kg N ha-1,分三叶(V3)和七叶(V7);F1,即“C0”,但在水中加入液肥;F2, 150 Kg N ha-1作为液肥,从V3期到繁殖期(R1)每周分水;F3,命名为“F2”,但添加225 Kg N ha-1。根据水分平衡进行灌溉,并用FDR探针测量土壤水分。在V3、V6、R1和生理成熟(R6)状态下评价植株氮素水平和生物量。粮食产量是在收获时确定的。利用航拍和软件分析R1中颜色值与颜色指数(InCol)的相关性,其中:% N;生物质能;氮充足指数;籽粒产量(kg ha-1)。施氮量最高的处理产量较高,但差异不显著。在评估变量中,没有发现氮源(硫脲和Fertec®)之间的差异。颜色与InCol和培养变量之间存在高度相关。
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引用次数: 0
Unraveling factors affecting consumers' liking of novel Uruguayan mandarins 揭示影响消费者对新型乌拉圭橘子喜爱的因素
Pub Date : 2021-08-18 DOI: 10.31285/agro.25.540
J. Lado, A. I. Moltini, P. Pintos, E. Luque, Lucía Goncalvez, F. Rivas, Florencia Alcaire, G. Ares
Mandarin cultivars show a great diversity on fruit-quality and sensory characteristics, together with an extended harvest season. Citrus breeding is focused on exploiting season niches for higher prices as well as nutritional and sensory aspects, among others. In this context, the sensory characterization of new hybrids and the identification of key characteristics of consumers´ liking provide valuable information towards breeding efforts and marketing strategies. Previous works showed that sensory characteristics, and specially flavor, play a key role in consumer acceptance of mandarins. Two studies were carried out during mandarin harvest season (mid and late) applying “check all that apply” (CATA) questions with at least 100 consumers. Overall liking scores of the most liked hybrids and cultivars ranged between 6.5 to 7.7 in the 9-point hedonic scale, evidencing a positive hedonic reaction. Local hybrids showed similar or higher values than the reference cultivars Tango, Gold Nugget, Murcott and Ortanique. Results showed that orange color, regular shape, smoothness, sweetness, juiciness, intense and typical flavor are the key drivers of consumer's liking of mandarins, and that external appearance is not a good predictor of mandarin tasting experience. Hybrids F3P8, F5P8 and F2P3 were described using terms related to positive sensory characteristics and received liking scores similar or higher to reference cultivars such as Tango, stressing the importance of sensory and consumer science as an integral part of breeding strategies.
柑桔品种在果实品质和感官特征上表现出极大的多样性,同时收获季节延长。柑橘育种的重点是开发季节利基,以获得更高的价格,以及营养和感官方面,等等。在这种情况下,新杂交种的感官特征和消费者喜欢的关键特征的识别为育种工作和营销策略提供了有价值的信息。以往的研究表明,感官特征,特别是味道,在消费者接受柑橘的过程中起着关键作用。在柑橘收获季节(中期和后期)进行了两项研究,对至少100名消费者进行了“检查所有适用的”(CATA)问题。在9分的享乐主义量表中,最受欢迎的杂种和品种的总体喜欢得分在6.5到7.7之间,表明了积极的享乐主义反应。地方杂交种表现出与参比品种Tango、Gold Nugget、Murcott和Ortanique相似或更高的值。结果表明,橘色、形状规则、光滑度、甜度、多汁性、浓郁和典型的味道是消费者喜欢柑橘的主要驱动因素,而外观并不能很好地预测柑橘的品尝体验。杂交品种F3P8、F5P8和F2P3使用与积极感官特性相关的术语进行描述,并获得与参考品种如Tango相似或更高的喜欢分数,强调了感官和消费者科学作为育种策略不可或缺的一部分的重要性。
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引用次数: 0
A major challenge for the Uruguayan dairy industry sustainable growth 乌拉圭乳业可持续增长面临的主要挑战
Pub Date : 2021-08-08 DOI: 10.31285/agro.25.970
P. Chilibroste
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引用次数: 2
Compatibility of Orius insidiosus (Hemiptera: Anthocoridae) with Amblyseius swirskii (Acari: Phytoseiidae) for control of Flankliniella occidentalis (Thysanoptera: Thripidae) in pepper 隐翅螟(半翅目:蠓科)与威氏钝绥螨(蜱螨目:蠓科)在辣椒中防治西翅螟的配伍性研究
Pub Date : 2021-07-29 DOI: 10.31285/agro.25.838
Maria Eugenia Lorenzo Larregui, Leticia Bao, G. Grille, L. Méndez, Oliver Bonato, C. Basso
Biological control programs in greenhouses increasingly resort to the release of several natural enemies simultaneously. In Uruguay, pest management in peppers is based on the release of Orius insidiosus and Amblyseius swirskii, to control Frankliniella occidentalis and Bemisia tabaci. Amblyseius swirskii can feed on both preys. Zoophytophagous predators not only feed on pests or plant food, but also on natural enemies with which they share prey (intraguild predation). Hence, the combined release of generalist predators could have a negative result in the control of the target pest. Thus, it was important to evaluate the compatibility of O. insidiosus and A. swirskii as predators of F. occidentalis. Studies carried out at Petri dishes level assessed: (1) the effect of the density and the composition of the prey on their predation by O. insidiosus, (2) the change of prey by O. insidiosus and (3) the effect of the genus of O. insidiosus on prey preference. Results show that if the relative densities of F. occidentalis and A. swirskii varied, O. insidiosus always changed to the most abundant prey. Male and female O. insidiosus preferred nymphs of F. occidentalis over A. swirskii, and females killed more prey than males. Since O. insidiosus fed on A. swirskii, it is advisable to be cautious when combining anthocorids and phytoseid mites for the F. occidentalis control. Studies at greenhouse will be necessary to clarify the final effect of both predators.
温室中的生物防治项目越来越多地采用同时释放几种天敌的方法。在乌拉圭,对辣椒进行虫害管理的基础是释放阴险稻瘟螨和威氏钝绥螨,以控制西方富兰克林蝇和烟粉虱。斯威氏钝绥螨可以以两种猎物为食。植食性捕食者不仅以害虫或植物为食,而且还以与它们共享猎物的天敌为食(野生动物捕食)。因此,综合释放多面手捕食者可能对目标害虫的控制产生负面影响。因此,对两种捕食者的相容性进行评价具有重要意义。研究在培养皿水平进行评估:(1)的密度和成分的影响由o . insidiosus捕食的猎物,(2)由o . insidiosus猎物的变化,(3)的影响属o . insidiosus猎物的偏好。结果表明,在西蠓与黄蠓相对密度发生变化的情况下,黑蠓总是向最丰富的猎物转变。雄、雌隐蝽对西蠓若虫的偏好高于雄蠓,且雌蚊对猎物的杀伤多于雄蚊。由于西腹粉蚧以黄纹姬螨为食,故在联合使用花甲螨和植籽螨防治西腹粉蚧时应谨慎。为了弄清这两种捕食者的最终影响,有必要在温室里进行研究。
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引用次数: 1
Evaluation of technical production in agricultural sciences a new certification scheme in Uruguay 农业科学技术生产评价乌拉圭新的认证计划
Pub Date : 2021-07-10 DOI: 10.31285/agro.25.491
Federico Vasen, M. Sierra, J. Paruelo, C. Negro, Federico Nolla, Joaquín Lapetina, Marcelo Salvagno
The evaluation of technological production in agricultural sciences presents specific challenges. Unlike scientific publications, for which there are standardized evaluation criteria, technological developments require a more multidimensional and situated approach. This article analyzes a technology certification scheme developed by the National Institute of Agricultural Research (INIA by its Spanish acronym) in Uruguay. The process aims to validate the developments based on the perspective of potential users. Based on a literature review and interviews with participants, we reviewed the process design and the first implementation round in 2019. Given the study results, we reported on the innovative nature of the process, both at national and regional levels. At the same time, we highlight the importance of incorporating a variety of stakeholders and prioritizing feedback and learning over bureaucratic control. Finally, we recommend linking this process with analogous instances that may exist in other institutions within the local science system.
农业科学技术成果的评价提出了具体的挑战。与有标准化评价标准的科学出版物不同,技术发展需要一种更多方面和更有针对性的方法。本文分析了乌拉圭国家农业研究所(INIA)开发的一项技术认证计划。这个过程的目的是根据潜在用户的观点来验证这些发展。基于文献综述和对参与者的访谈,我们回顾了2019年的流程设计和第一轮实施。鉴于研究结果,我们在国家和区域两级报告了这一进程的创新性质。与此同时,我们强调整合各种利益相关者以及优先考虑反馈和学习而不是官僚控制的重要性。最后,我们建议将这一过程与当地科学系统内其他机构中可能存在的类似实例联系起来。
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引用次数: 3
Assessing MODIS16A2 actual evapotranspiration across three spatial resolutions in Uruguay 评估乌拉圭三个空间分辨率的MODIS16A2实际蒸散量
Pub Date : 2021-06-25 DOI: 10.31285/agro.25.429
R. Navas, G. Tiscornia, A. Berger, Á. Otero
Evapotranspiration (ET) is a key process in hydrological systems and, consequently, in agroecosystems. It can be measured or derived with a large variety of models at scales ranging from leaf to catchment. MODIS16A2 is a satellite ET product with 500 meters / 8-day spatio-temporal resolution worldwide. It is based on the Penman-Monteith equation and considers the effect of vegetation dynamics, albedo and land cover. This technical paper compares the ET estimated from MODIS16A2 against the ET estimated at different scales from three reference methods: (1) the INIA-GRAS Water Balance on a country-scale, (2) the SWAT model of the Santa Lucia basin on the catchment scale, and (3) the Eddy Covariance Flux located in Colonia on a farmer scale. The analysis shows similarities between MODIS16A2 and the reference methods depending on seasonality, geographic location and scale of ET estimation. The assumptions about vegetation cover, vegetation dynamics, meteorological forcing and soil characteristics of the reference methods compared with MODIS16A2 ones could explain some deviations in the ET estimations. The results of this work contribute with a first approximation towards the quantification of the uncertainty of MODIS16A2 in Uruguay.
蒸散发(ET)是水文系统的关键过程,因此也是农业生态系统的关键过程。它可以用从叶片到流域的各种尺度的模型来测量或推导。MODIS16A2是全球500米/ 8天时空分辨率的卫星ET产品。该模型以Penman-Monteith方程为基础,考虑了植被动态、反照率和土地覆盖的影响。本文将MODIS16A2估算的ET与三种参考方法估算的不同尺度的ET进行了比较:(1)国家尺度上的nia - gras水平衡,(2)流域尺度上的Santa Lucia流域SWAT模型,以及(3)农民尺度上的Colonia涡旋相关通量。分析表明,MODIS16A2与参考方法在季节性、地理位置和ET估算尺度等方面存在相似之处。参考方法对植被覆盖、植被动态、气象强迫和土壤特征的假设与MODIS16A2方法的比较可以解释蒸散发估算的一些偏差。这项工作的结果有助于对乌拉圭MODIS16A2不确定性的量化作出初步近似。
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引用次数: 1
Uruguayan consumers' perception of mandarins insights for selection and marketing of new cultivars 乌拉圭消费者对柑桔的认知对新品种选择和营销的见解
Pub Date : 2021-06-06 DOI: 10.31285/agro.25.530
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引用次数: 1
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Agrociencia-Uruguay
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