Ternary metal sulfides with suitable band gaps, high physicochemical stability, and unique two-dimensional (2D) nanostructures are expected to be the next-generation high-performance gas sensors following the MOS type. Doping engineering is utilized as an effective strategy to improve the semiconductor surface activity and enhance its gas-sensitive properties. In this paper, the energy band structure and surface chemical oxygen of ZnIn2S4 (ZIS) materials was tuned by selectively introducing substitutional Cd to replace the Zn sites in ZIS crystals. Meanwhile, the introduction of Cd-ions brings more abundant S vacancy defects, enhances the acid-base interactions at the interface, and pushes the extent of surface redox reactions. In addition, by combining the strong adsorption of ZIS to triethylamine, the CdxZn1-xIn2S4 nanosheets achieved highly improved sensing properties, including better response (63.38-100 ppm), enhanced selectivity (STEA/sother = 12.9), and accelerated response/recovery (4 s/32 s). The results confirm the feasibility of developing low-cost, high-performance 2D metal sulfide gas sensing materials through rational structural design and optimization.
三元金属硫化物具有合适的带隙、较高的物理化学稳定性和独特的二维(2D)纳米结构,有望成为继 MOS 型之后的下一代高性能气体传感器。掺杂工程是提高半导体表面活性和增强其气敏特性的有效策略。本文通过选择性地引入替代性镉来取代 ZIS 晶体中的锌位点,从而调整了 ZnIn2S4(ZIS)材料的能带结构和表面化学氧。同时,Cd 离子的引入带来了更丰富的 S 空位缺陷,增强了界面上的酸碱相互作用,推动了表面氧化还原反应的程度。此外,结合 ZIS 对三乙胺的强吸附性,CdxZn1-xIn2S4 纳米片实现了高度改进的传感性能,包括更好的响应(63.38-100 ppm)、更高的选择性(STEA/sother = 12.9)和更快的响应/恢复(4 s/32 s)。这些结果证实了通过合理的结构设计和优化开发低成本、高性能二维金属硫化物气体传感材料的可行性。
{"title":"The synergistic effect of Cd-doped and S-vacancies in Cd<sub>x</sub>Zn<sub>1-x</sub>In<sub>2</sub>S<sub>4</sub> 2D nanosheets for high-performance triethylamine sensing.","authors":"Yizhuo Fan, Jian Fang, Wei Wang, Qilin Wang, Dali Liu, Yu Chen, Shengping Ruan","doi":"10.1016/j.talanta.2024.126625","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126625","url":null,"abstract":"<p><p>Ternary metal sulfides with suitable band gaps, high physicochemical stability, and unique two-dimensional (2D) nanostructures are expected to be the next-generation high-performance gas sensors following the MOS type. Doping engineering is utilized as an effective strategy to improve the semiconductor surface activity and enhance its gas-sensitive properties. In this paper, the energy band structure and surface chemical oxygen of ZnIn<sub>2</sub>S<sub>4</sub> (ZIS) materials was tuned by selectively introducing substitutional Cd to replace the Zn sites in ZIS crystals. Meanwhile, the introduction of Cd-ions brings more abundant S vacancy defects, enhances the acid-base interactions at the interface, and pushes the extent of surface redox reactions. In addition, by combining the strong adsorption of ZIS to triethylamine, the Cd<sub>x</sub>Zn<sub>1-x</sub>In<sub>2</sub>S<sub>4</sub> nanosheets achieved highly improved sensing properties, including better response (63.38-100 ppm), enhanced selectivity (S<sub>TEA</sub>/s<sub>other</sub> = 12.9), and accelerated response/recovery (4 s/32 s). The results confirm the feasibility of developing low-cost, high-performance 2D metal sulfide gas sensing materials through rational structural design and optimization.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, a nanozyme (ZIF-Co-Cys) with high oxidase-like catalytic activity was prepared, and a ratiometric fluorescent/photothermal dual-mode probe was constructed for organophosphorus pesticides (OPs) detection based on the competitive effect of ZIF-Co-Cys and the enzymatic reaction product of acid phosphatase (ACP) on o-phenylenediamine and the inhibition effect of OPs on ACP activity. Using dimethyl dichloroviny phosphate (DDVP) as the model, both the fluorescence intensity ratio and the temperature change of the probe solution exhibited an excellent correlation with OPs concentration. The detection limits were 1.64 ng/mL and 0.084 ng/mL, respectively. Additionally, the detection of DDVP residues in real samples verified the outstanding anti-interference and accuracy of the probe. This work not only provided a complementary dual-mode method for the accurate and rapid detection of OPs residues in complex samples, but also supplied a new insight into the design of a multi-mode sensing platform based on the cascade reaction of nanozyme.
{"title":"A novel fluorescent and photothermal probe based on nanozyme-mediated cascade reaction for detecting organophosphorus pesticide residues.","authors":"Siyu Zhang, Zheng Wang, Yingying Feng, Chuang Jiang, Hui Li, Zhenyu Yu, Yaqing Xiao, Ruyan Hou, Xiaochun Wan, Yingnan Liu","doi":"10.1016/j.talanta.2024.126620","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126620","url":null,"abstract":"<p><p>In this study, a nanozyme (ZIF-Co-Cys) with high oxidase-like catalytic activity was prepared, and a ratiometric fluorescent/photothermal dual-mode probe was constructed for organophosphorus pesticides (OPs) detection based on the competitive effect of ZIF-Co-Cys and the enzymatic reaction product of acid phosphatase (ACP) on o-phenylenediamine and the inhibition effect of OPs on ACP activity. Using dimethyl dichloroviny phosphate (DDVP) as the model, both the fluorescence intensity ratio and the temperature change of the probe solution exhibited an excellent correlation with OPs concentration. The detection limits were 1.64 ng/mL and 0.084 ng/mL, respectively. Additionally, the detection of DDVP residues in real samples verified the outstanding anti-interference and accuracy of the probe. This work not only provided a complementary dual-mode method for the accurate and rapid detection of OPs residues in complex samples, but also supplied a new insight into the design of a multi-mode sensing platform based on the cascade reaction of nanozyme.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-25DOI: 10.1016/j.talanta.2024.126597
Tianyu Liang, Xinyu Chen, Shuling Liu, Hanyang Li, Tianli Du, Yang Li, Mingyu Tian, Chengyan Wu, Xiaofei Sun, Taotao Qiang, Keli Zhong, Lijun Tang
As an important ROS species participating in various physiological and pathological processes, high level of hypochlorite (ClO-) poses significant health and safety concerns, necessitating efficient detection methods. Herein, this study introduces a water-soluble fluorescent nanoprobe Nano-SJD, effectively detect ClO- in both food samples and living cells. The small molecular probe SJD with N, N-dimethylthiocarbamyl (DMTC) as recognition moiety was constructed based on a naphthalene derivative. To further improve the water solubility, SJD was assembled with an amphiphilic copolymer (mPEG-DSPE) to prepare a water soluble fluorescent nanoprobe Nano-SJD. Fortunately, the nanoprobe preserves the excellent properties of small molecules and performs very well optical response to ClO- in aqueous solution, possessing the advantages including ultra-rapid response (within 1 s), minimal interference, low detection limits (0.39 μM) and good pH stability. What's more important, we have also developed smartphone-compatible test paper strips for convenient on-site detection of ClO- in real-water samples. Additionally, the robust fluorescent imaging behavior of Nano-SJD for visualization of ClO- in living cells highlights its broad potential in biosystem applicability.
{"title":"A self-assembled nanoprobe for rapid detection of hypochlorite in pure water and its application in living cells, food and environmental systems.","authors":"Tianyu Liang, Xinyu Chen, Shuling Liu, Hanyang Li, Tianli Du, Yang Li, Mingyu Tian, Chengyan Wu, Xiaofei Sun, Taotao Qiang, Keli Zhong, Lijun Tang","doi":"10.1016/j.talanta.2024.126597","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126597","url":null,"abstract":"<p><p>As an important ROS species participating in various physiological and pathological processes, high level of hypochlorite (ClO<sup>-</sup>) poses significant health and safety concerns, necessitating efficient detection methods. Herein, this study introduces a water-soluble fluorescent nanoprobe Nano-SJD, effectively detect ClO<sup>-</sup> in both food samples and living cells. The small molecular probe SJD with N, N-dimethylthiocarbamyl (DMTC) as recognition moiety was constructed based on a naphthalene derivative. To further improve the water solubility, SJD was assembled with an amphiphilic copolymer (mPEG-DSPE) to prepare a water soluble fluorescent nanoprobe Nano-SJD. Fortunately, the nanoprobe preserves the excellent properties of small molecules and performs very well optical response to ClO<sup>-</sup> in aqueous solution, possessing the advantages including ultra-rapid response (within 1 s), minimal interference, low detection limits (0.39 μM) and good pH stability. What's more important, we have also developed smartphone-compatible test paper strips for convenient on-site detection of ClO<sup>-</sup> in real-water samples. Additionally, the robust fluorescent imaging behavior of Nano-SJD for visualization of ClO<sup>-</sup> in living cells highlights its broad potential in biosystem applicability.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-25DOI: 10.1016/j.talanta.2024.126573
Viktor V Pervukhin, Dmitriy G Sheven
A method is proposed for increasing the number of ions during mass-spectrometric analysis of samples in a nonpolar solvent (benzene). For this purpose, aerodynamic thermal breakup droplet ionization (ATBDI) with the impact of β-radiation on the aerosol droplets used in ATBDI was evaluated. This modification of the method, which we named β-ATBDI, allows to shift a nonvolatile analyte (trinitrotoluene in the negative ionization region and cocaine in the positive ionization region, as an example) into a gas phase as an aerosol at room temperature (in contrast to atmospheric pressure chemical ionization). In addition, β-ATBDI enables a researcher to distinguish mass spectrometric peaks of the compounds located in an aerosol droplet from compounds located outside the droplet, i.e., to identify background peaks. Also briefly discussed the ionization of two antibiotics-azithromycin in methylene chloride and sulfadiazine in salt water with β-ATBDI, ATBDI and electrospray ionization source.
{"title":"Aerodynamic thermal breakup droplet ionization combined with a β-irradiation source for mass-spectrometric analysis of samples in a nonpolar solvent.","authors":"Viktor V Pervukhin, Dmitriy G Sheven","doi":"10.1016/j.talanta.2024.126573","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126573","url":null,"abstract":"<p><p>A method is proposed for increasing the number of ions during mass-spectrometric analysis of samples in a nonpolar solvent (benzene). For this purpose, aerodynamic thermal breakup droplet ionization (ATBDI) with the impact of β-radiation on the aerosol droplets used in ATBDI was evaluated. This modification of the method, which we named β-ATBDI, allows to shift a nonvolatile analyte (trinitrotoluene in the negative ionization region and cocaine in the positive ionization region, as an example) into a gas phase as an aerosol at room temperature (in contrast to atmospheric pressure chemical ionization). In addition, β-ATBDI enables a researcher to distinguish mass spectrometric peaks of the compounds located in an aerosol droplet from compounds located outside the droplet, i.e., to identify background peaks. Also briefly discussed the ionization of two antibiotics-azithromycin in methylene chloride and sulfadiazine in salt water with β-ATBDI, ATBDI and electrospray ionization source.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-25DOI: 10.1016/j.talanta.2024.126619
Denise Biagini, Simona Mrakic-Sposta, Danilo Bondi, Silvia Ghimenti, Alessio Lenzi, Federico Vivaldi, Carmen Santangelo, Vittore Verratti, Tiziana Pietrangelo, Alessandra Vezzoli, Guido Giardini, Camille Oger, Jean-Marie Galano, Laurence Balas, Thierry Durand, Gennaro D'Angelo, Tommaso Lomonaco, Fabio Di Francesco
Oxylipins are powerful signalling compounds derived from polyunsaturated fatty acids (PUFAs) and involved in regulating the immune system response. A mass spectrometry-based method was developed and validated for the targeted profiling of 52 oxylipins (e.g., isoprostanoids, prostaglandins, epoxy- and hydroxy-fatty acids, specialized pro-resolving mediators) and 4 PUFAs in small urinary extracellular vesicles (uEVs). Ultrasound-assisted extraction using a 50:50 v/v MeOH:H2O mixture ensured optimal analytical performances. Limits of detection ranged between 10 and 400 pg/mL for oxylipins and 0.10-3 ng/mL for PUFAs. Satisfactory recoveries (85-116 %) and good intra- and inter-day precisions (RSD ≤15 %) were obtained for all the analytes. The reliability of the procedure was tested in a real case scenario by monitoring ultramarathon runners during the world Tor des Géants® (TDG) race. Both F2- and E2-isoprostanes were detected in small uEVs of the ultramarathon runners, suggesting the onset of an oxidant insult. 5-F2t-IsoP exhibited significant pre- to post-race variations, thus potentially representing a non-invasive marker of in-vivo lipid peroxidation. The presence of specialized pro-resolving mediators suggests the activation of pro-resolution signalling cascade resolving inflammation. These outcomes may help manage post-exercise recovery and improve training.
{"title":"A MEPS-UHPLC-MS/MS analytical platform to detect isoprostanoids and specialized pro-resolving mediators in the urinary extracellular vesicles of mountain ultramarathon runners.","authors":"Denise Biagini, Simona Mrakic-Sposta, Danilo Bondi, Silvia Ghimenti, Alessio Lenzi, Federico Vivaldi, Carmen Santangelo, Vittore Verratti, Tiziana Pietrangelo, Alessandra Vezzoli, Guido Giardini, Camille Oger, Jean-Marie Galano, Laurence Balas, Thierry Durand, Gennaro D'Angelo, Tommaso Lomonaco, Fabio Di Francesco","doi":"10.1016/j.talanta.2024.126619","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126619","url":null,"abstract":"<p><p>Oxylipins are powerful signalling compounds derived from polyunsaturated fatty acids (PUFAs) and involved in regulating the immune system response. A mass spectrometry-based method was developed and validated for the targeted profiling of 52 oxylipins (e.g., isoprostanoids, prostaglandins, epoxy- and hydroxy-fatty acids, specialized pro-resolving mediators) and 4 PUFAs in small urinary extracellular vesicles (uEVs). Ultrasound-assisted extraction using a 50:50 v/v MeOH:H<sub>2</sub>O mixture ensured optimal analytical performances. Limits of detection ranged between 10 and 400 pg/mL for oxylipins and 0.10-3 ng/mL for PUFAs. Satisfactory recoveries (85-116 %) and good intra- and inter-day precisions (RSD ≤15 %) were obtained for all the analytes. The reliability of the procedure was tested in a real case scenario by monitoring ultramarathon runners during the world Tor des Géants® (TDG) race. Both F<sub>2</sub>- and E<sub>2</sub>-isoprostanes were detected in small uEVs of the ultramarathon runners, suggesting the onset of an oxidant insult. 5-F<sub>2t</sub>-IsoP exhibited significant pre- to post-race variations, thus potentially representing a non-invasive marker of in-vivo lipid peroxidation. The presence of specialized pro-resolving mediators suggests the activation of pro-resolution signalling cascade resolving inflammation. These outcomes may help manage post-exercise recovery and improve training.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Peptidyl arginine deiminase 4 (PAD4) plays a critical role in many autoimmune diseases including rheumatoid arthritis. Herein, a trypsin assisted highly immunoassay method was established to determine PAD4 activity and screen potent inhibitors from herbal plants extracts and purified natural products. The method was applied to determine endogenous PAD4 activity in both cell and tissue lysates, as well as the inhibitory effects of 20 herbal plants and 50 purified natural products. The Cinnamomi ramulus extract showed strongest inhibitory potency with IC50 value lower than 5 μg/mL. Meanwhile, pyrroloquinoline quinone (PQQ), widely used as a dietary supplement, was discovered as a promising PAD4 inhibitor with an IC50 value lower than 4 μM. The inhibition kinetic analysis, drug affinity response target stability (DARTS) and molecular docking were performed to confirm the interaction between PQQ and PAD4. This method has great potential for researchers to monitor activities and discover potential inhibitors of PAD4.
{"title":"Screening and identification of peptidyl arginine deiminase 4 inhibitors from herbal plants extracts and purified natural products by a trypsin assisted sensitive immunoassay based on streptavidin magnetic beads.","authors":"Juanjuan Zhao, Shengxiang Zhang, Jianhui Dong, Xufei Chen, Haiyue Zuo, Yanfeng Li, Chunli Gao, Zeyuan Zhao, Xingtai Qiu, Zichao Tang, Nan Deng, Weining Zhao, Junjie Ou, Yangyang Bian","doi":"10.1016/j.talanta.2024.126611","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126611","url":null,"abstract":"<p><p>Peptidyl arginine deiminase 4 (PAD4) plays a critical role in many autoimmune diseases including rheumatoid arthritis. Herein, a trypsin assisted highly immunoassay method was established to determine PAD4 activity and screen potent inhibitors from herbal plants extracts and purified natural products. The method was applied to determine endogenous PAD4 activity in both cell and tissue lysates, as well as the inhibitory effects of 20 herbal plants and 50 purified natural products. The Cinnamomi ramulus extract showed strongest inhibitory potency with IC<sub>50</sub> value lower than 5 μg/mL. Meanwhile, pyrroloquinoline quinone (PQQ), widely used as a dietary supplement, was discovered as a promising PAD4 inhibitor with an IC<sub>50</sub> value lower than 4 μM. The inhibition kinetic analysis, drug affinity response target stability (DARTS) and molecular docking were performed to confirm the interaction between PQQ and PAD4. This method has great potential for researchers to monitor activities and discover potential inhibitors of PAD4.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-24DOI: 10.1016/j.talanta.2024.126616
Jesús Marín-Sáez, Maykel Hernández-Mesa, Germán Cano-Sancho, Ana M García-Campaña
Exposomics aims to measure human exposures throughout the lifespan and the changes they produce in the human body. Exposome-scale studies have significant potential to understand the interplay of environmental factors with complex multifactorial diseases widespread in our society and whose origin remain unclear. In this framework, the study of the chemical exposome aims to cover all chemical exposures and their effects in human health but, today, this goal still seems unfeasible or at least very challenging, which makes the exposome for now only a concept. Furthermore, the study of the chemical exposome faces several methodological challenges such as moving from specific targeted methodologies towards high-throughput multitargeted and non-targeted approaches, guaranteeing the availability and quality of biological samples to obtain quality analytical data, standardization of applied analytical methodologies, as well as the statistical assignment of increasingly complex datasets, or the identification of (un)known analytes. This review discusses the various steps involved in applying the exposome concept from an analytical perspective. It provides an overview of the wide variety of existing analytical methods and instruments, highlighting their complementarity to develop combined analytical strategies to advance towards the chemical exposome characterization. In addition, this review focuses on endocrine disrupting chemicals (EDCs) to show how studying even a minor part of the chemical exposome represents a great challenge. Analytical strategies applied in an exposomics context have shown great potential to elucidate the role of EDCs in health outcomes. However, translating innovative methods into etiological research and chemical risk assessment will require a multidisciplinary effort. Unlike other review articles focused on exposomics, this review offers a holistic view from the perspective of analytical chemistry and discuss the entire analytical workflow to finally obtain valuable results.
{"title":"Analytical challenges and opportunities in the study of endocrine disrupting chemicals within an exposomics framework.","authors":"Jesús Marín-Sáez, Maykel Hernández-Mesa, Germán Cano-Sancho, Ana M García-Campaña","doi":"10.1016/j.talanta.2024.126616","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126616","url":null,"abstract":"<p><p>Exposomics aims to measure human exposures throughout the lifespan and the changes they produce in the human body. Exposome-scale studies have significant potential to understand the interplay of environmental factors with complex multifactorial diseases widespread in our society and whose origin remain unclear. In this framework, the study of the chemical exposome aims to cover all chemical exposures and their effects in human health but, today, this goal still seems unfeasible or at least very challenging, which makes the exposome for now only a concept. Furthermore, the study of the chemical exposome faces several methodological challenges such as moving from specific targeted methodologies towards high-throughput multitargeted and non-targeted approaches, guaranteeing the availability and quality of biological samples to obtain quality analytical data, standardization of applied analytical methodologies, as well as the statistical assignment of increasingly complex datasets, or the identification of (un)known analytes. This review discusses the various steps involved in applying the exposome concept from an analytical perspective. It provides an overview of the wide variety of existing analytical methods and instruments, highlighting their complementarity to develop combined analytical strategies to advance towards the chemical exposome characterization. In addition, this review focuses on endocrine disrupting chemicals (EDCs) to show how studying even a minor part of the chemical exposome represents a great challenge. Analytical strategies applied in an exposomics context have shown great potential to elucidate the role of EDCs in health outcomes. However, translating innovative methods into etiological research and chemical risk assessment will require a multidisciplinary effort. Unlike other review articles focused on exposomics, this review offers a holistic view from the perspective of analytical chemistry and discuss the entire analytical workflow to finally obtain valuable results.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-24DOI: 10.1016/j.talanta.2024.126610
Ahmed Z Alanazi, Khalid Alhazzani, Mohamed M El-Wekil, Al-Montaser Bellah H Ali, Mahmoud Darweesh, Hossieny Ibrahim
For the first time, we introduce a novel disposable and ultrasensitive sensing electrode made up of nanosized ceria uniformly loaded carbon nanofibers (CeNPs@CNF) sol-gel nanoceramic film (CF) wrapped on eco-friendly and inexpensive pencil graphite rods (PGRs) to explore their electro-catalytic detection of the anticancer drug capmatinib (CMB). The as-prepared CeNPs@CNF hybrid nanocomposite was described by XRD, SEM, TEM, HRTEM, and EDX analysis. The CV study clearly demonstrated that, the disposable CeNPs@CNF-CF/PGRE sensor exhibited excellent redox activities in the ideal probe [Fe(CN)6]3-/4-. Due to the outstanding electrochemical properties, larger electrochemically active surface area, and tremendous electro-catalytic activity of CeNPs@CNF, the reduction current of CMB on the CeNPs@CNF-CF/PGRE sensor is considerably higher than that of bare PGRE. The detection conditions, such as supporting electrolyte, pH of the buffer solution, amount of modifier, adsorption potential, and time, were studied and optimized. The sensing platform demonstrated high sensitivity (1.2 μA nM-1 cm-2), an ultralow detection limit (0.6 nM), and a wide linear range of 2.0 nM-400 nM of CMB compared to the bare PGRE. Additionally, the CeNPs@CNF-CF/PGRE sensor showed high selectivity, stability, and simple operation, which provided a promising alternative tool for fast detection of CMB in human body fluids with good recoveries.
{"title":"A novel disposable ultrasensitive sensor based on nanosized ceria uniformly loaded carbon nanofiber nanoceramic film wrapped on pencil graphite rods for electrocatalytic monitoring of a tyrosine kinase inhibitor capmatinib.","authors":"Ahmed Z Alanazi, Khalid Alhazzani, Mohamed M El-Wekil, Al-Montaser Bellah H Ali, Mahmoud Darweesh, Hossieny Ibrahim","doi":"10.1016/j.talanta.2024.126610","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126610","url":null,"abstract":"<p><p>For the first time, we introduce a novel disposable and ultrasensitive sensing electrode made up of nanosized ceria uniformly loaded carbon nanofibers (CeNPs@CNF) sol-gel nanoceramic film (CF) wrapped on eco-friendly and inexpensive pencil graphite rods (PGRs) to explore their electro-catalytic detection of the anticancer drug capmatinib (CMB). The as-prepared CeNPs@CNF hybrid nanocomposite was described by XRD, SEM, TEM, HRTEM, and EDX analysis. The CV study clearly demonstrated that, the disposable CeNPs@CNF-CF/PGRE sensor exhibited excellent redox activities in the ideal probe [Fe(CN)<sub>6</sub>]<sup>3-/4-</sup>. Due to the outstanding electrochemical properties, larger electrochemically active surface area, and tremendous electro-catalytic activity of CeNPs@CNF, the reduction current of CMB on the CeNPs@CNF-CF/PGRE sensor is considerably higher than that of bare PGRE. The detection conditions, such as supporting electrolyte, pH of the buffer solution, amount of modifier, adsorption potential, and time, were studied and optimized. The sensing platform demonstrated high sensitivity (1.2 μA nM<sup>-1</sup> cm<sup>-2</sup>), an ultralow detection limit (0.6 nM), and a wide linear range of 2.0 nM-400 nM of CMB compared to the bare PGRE. Additionally, the CeNPs@CNF-CF/PGRE sensor showed high selectivity, stability, and simple operation, which provided a promising alternative tool for fast detection of CMB in human body fluids with good recoveries.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-23DOI: 10.1016/j.talanta.2024.126561
Yu-Yao Cao, Song-Yu Wu, Liang-Chao Yuan, Wan Su, Xin-Yue Chen, Jian-Cheng Pan, Ya-Xi Ye, Qing-Cai Jiao, Hai-Liang Zhu
Acute lung injury (ALI) is a serious pulmonary inflammatory disease resulting from excessive reactive oxygen species (ROS) which could cause the damage of the alveolar epithelial cells and capillary endothelial cells. Peroxynitrite, as one of short-lived reactive oxygen species, is closely related to the process of ALI. Thus, it is important to monitor the fluctuation of peroxynitrite in living system for understanding the process of ALI. Herein, the novel mitochondria-targeted fluorescent probe BHMT was designed to respond to peroxynitrite and pH with distinct fluorescence properties respectively. The absorption spectrum of the probe BHMT exhibited a notable red shift as the pH value declined from 8.8 to 2.6. Upon reaction with peroxynitrite, BHMT had a significant increase of fluorescence intensity (63-fold) with maintaining a detection limit of only 43.7 nM. Furthermore, BHMT could detect the levels of endogenous peroxynitrite and image the intracellular pH in ratiometric channels utilizing cell imaging. In addition, BHMT was successfully applied to revealing the relationship between the peroxynitrite and the extent of ALI. Thus, these results indicated the probe BHMT could be a potential tool for diagnosing the early stage of ALI and revealed the peroxynitrite was likely to be a crucial therapeutic target in ALI treatment.
急性肺损伤(ALI)是一种严重的肺部炎症性疾病,由过量的活性氧(ROS)导致肺泡上皮细胞和毛细血管内皮细胞损伤。过氧化亚硝酸盐作为短寿命活性氧之一,与 ALI 的发生过程密切相关。因此,监测活体系统中过亚硝酸盐的波动对了解 ALI 的过程非常重要。本文设计了新型线粒体靶向荧光探针 BHMT,该探针分别对过亚硝酸盐和 pH 具有不同的荧光特性。当 pH 值从 8.8 降到 2.6 时,探针 BHMT 的吸收光谱出现明显的红移。与过亚硝酸盐反应后,BHMT 的荧光强度显著增加(63 倍),而检测限仅为 43.7 nM。此外,BHMT 还能检测内源性过氧化亚硝酸盐的水平,并利用细胞成像技术在比率通道中对细胞内 pH 值进行成像。此外,BHMT 还被成功应用于揭示过氧化亚硝酸盐与 ALI 程度之间的关系。因此,这些结果表明 BHMT 探针是诊断 ALI 早期阶段的潜在工具,并揭示了过氧化亚硝酸盐可能是治疗 ALI 的关键治疗靶点。
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