Pub Date : 2025-06-14DOI: 10.1016/j.talo.2025.100504
Karla R. Castro , Isabela A. Mattioli , Graziela C. Sedenho , Thiago Bertaglia , Antônio F.A. Araújo , Manoel J.A. Lima , Beatriz G.R. da Silva , Mona N. Oliveira , Iris Todeschini , Phelipe M. Vitale , Erika R. Manuli , Geovana M. Pereira , Suzete C. Ferreira , Ester C. Sabino , Emanuel Carrilho , Frank N. Crespilho
Lateral flow immunoassays (LFIAs) have modernized decentralized mass testing and significantly impacted the healthcare system. It provides a rapid and accessible method for serological testing, which is an important tool for screening patients with past viral phases by detecting antibodies. Here, the development of a quantitative LFIA is presented, in which the receptor-binding domain of Spike protein-specific antibody (anti-S-RBD) in serum samples from previously infected with SARS-CoV-2 patients form an immune complex with the RBD protein immobilized on gold nanoparticles. For quantitative measurements, a methodology was developed based on the plasmonic behavior of gold nanoparticles and using a digital camera that processes images of the test lines in a closed chamber using the CYMK color system. The limits of detection and quantification were 0.22 and 0.70 µg mL–1, respectively. The proposed device has a manufacturing cost of less than US$ 1 from synthesizing the bioconjugates, assembly of the LFIA strips, and 3D printing of the cassettes. The platform could detect anti-S-RBD antibodies in human serum samples and can be particularly useful in strategies for monitoring IgG levels in the population.
{"title":"Plasmonic and quantitative lateral flow assay for grayscale imaging of neutralizing antibodies SARS-CoV-2","authors":"Karla R. Castro , Isabela A. Mattioli , Graziela C. Sedenho , Thiago Bertaglia , Antônio F.A. Araújo , Manoel J.A. Lima , Beatriz G.R. da Silva , Mona N. Oliveira , Iris Todeschini , Phelipe M. Vitale , Erika R. Manuli , Geovana M. Pereira , Suzete C. Ferreira , Ester C. Sabino , Emanuel Carrilho , Frank N. Crespilho","doi":"10.1016/j.talo.2025.100504","DOIUrl":"10.1016/j.talo.2025.100504","url":null,"abstract":"<div><div>Lateral flow immunoassays (LFIAs) have modernized decentralized mass testing and significantly impacted the healthcare system. It provides a rapid and accessible method for serological testing, which is an important tool for screening patients with past viral phases by detecting antibodies. Here, the development of a quantitative LFIA is presented, in which the receptor-binding domain of Spike protein-specific antibody (anti-S-RBD) in serum samples from previously infected with SARS-CoV-2 patients form an immune complex with the RBD protein immobilized on gold nanoparticles. For quantitative measurements, a methodology was developed based on the plasmonic behavior of gold nanoparticles and using a digital camera that processes images of the test lines in a closed chamber using the CYMK color system. The limits of detection and quantification were 0.22 and 0.70 µg mL<sup>–1</sup>, respectively. The proposed device has a manufacturing cost of less than US$ 1 from synthesizing the bioconjugates, assembly of the LFIA strips, and 3D printing of the cassettes. The platform could detect anti-S-RBD antibodies in human serum samples and can be particularly useful in strategies for monitoring IgG levels in the population.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100504"},"PeriodicalIF":4.1,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-13DOI: 10.1016/j.talo.2025.100502
Eman Yosrey , Heba Elmansi , Shereen Shalan , Jenny Jeehan Nasr
In this work, we present two label-free spectrofluorimetric systems for the swift quantification of vincamine (VN) in various matrices without the need for knotty fluorescent dyes, drastic conditions, or expensive instrumentation, which are key merits of the quantifying procedures. The first method (Method I) relied on the direct mix-and-read assay for exploiting the intrinsic fluorescence of VN, adopting λex/em of 272/334 nm using water as a diluting solvent. The method fitted linearity across the concentrations of 1.00 – 10.00 µg mL−¹. In the second method (Method II), upon turning off the photoinduced electron transfer (PET) of tertiary amine in the piperidine moiety to the indole scaffold using 0.1 M hydrochloric acid, the fluorescent signal was augmented up to 158 %. This commendable boost in the fluorescent sensitivity allowed the minute detection of VN in spiked human plasma with a standard deviation not exceeding 1.30. Using the acid-blocked PET-based probe, the fluorescent signal was linearly correlated with VN concentration across 0.25 – 6.00 µg mL−¹ using λex/em of 277/323 nm. These streamlined procedures proficiently evaluated VN in both the sole and co-formulated capsules without any significant interference from capsule filler material or the co-formulated medication “piracetam”. In addition, the two submitted procedures were successfully executed to check VN through weight variation testing in the Brain-ox® capsules with good acceptance values. The constructed platforms ensemble estimated VN in the environmental samples (tap and river water) with acceptable percentage recoveries and standard deviations (0.95 – 1.65). The procedures' green, white, and blue fitness were appraised through a plethora of computational tools, including GAPI, AGREE, AGREEprep, the RGB 12 algorithm, and BAGI. The evaluation showed that the proposed works effectively strike a compromise between sustainability and functionality postulates. This highlighted that designed approaches are perfectly suited as ecological frameworks for minuscule assaying of VN in crucial pharmaceutical, biological, and environmental samples.
{"title":"Label-free masterful spectrofluorimetric systems for minuscule tracking of natural antioxidant vincamine in the pharmaceutical, environmental, and biological samples: Comparative greenness, whiteness, and blueness appraisal","authors":"Eman Yosrey , Heba Elmansi , Shereen Shalan , Jenny Jeehan Nasr","doi":"10.1016/j.talo.2025.100502","DOIUrl":"10.1016/j.talo.2025.100502","url":null,"abstract":"<div><div>In this work, we present two label-free spectrofluorimetric systems for the swift quantification of vincamine (VN) in various matrices without the need for knotty fluorescent dyes, drastic conditions, or expensive instrumentation, which are key merits of the quantifying procedures. The first method (Method I) relied on the direct mix-and-read assay for exploiting the intrinsic fluorescence of VN, adopting λ<sub>ex/em</sub> of 272/334 nm using water as a diluting solvent. The method fitted linearity across the concentrations of 1.00 – 10.00 µg mL<sup>−</sup>¹. In the second method (Method II), upon turning off the photoinduced electron transfer (PET) of tertiary amine in the piperidine moiety to the indole scaffold using 0.1 M hydrochloric acid, the fluorescent signal was augmented up to 158 %. This commendable boost in the fluorescent sensitivity allowed the minute detection of VN in spiked human plasma with a standard deviation not exceeding 1.30. Using the acid-blocked PET-based probe, the fluorescent signal was linearly correlated with VN concentration across 0.25 – 6.00 µg mL<sup>−</sup>¹ using λ<sub>ex/em</sub> of 277/323 nm. These streamlined procedures proficiently evaluated VN in both the sole and co-formulated capsules without any significant interference from capsule filler material or the co-formulated medication “piracetam”. In addition, the two submitted procedures were successfully executed to check VN through weight variation testing in the Brain-ox® capsules with good acceptance values. The constructed platforms ensemble estimated VN in the environmental samples (tap and river water) with acceptable percentage recoveries and standard deviations (0.95 – 1.65). The procedures' green, white, and blue fitness were appraised through a plethora of computational tools, including GAPI, AGREE, AGREEprep, the RGB 12 algorithm, and BAGI. The evaluation showed that the proposed works effectively strike a compromise between sustainability and functionality postulates. This highlighted that designed approaches are perfectly suited as ecological frameworks for minuscule assaying of VN in crucial pharmaceutical, biological, and environmental samples.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100502"},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-09DOI: 10.1016/j.talo.2025.100497
Reshma P R, Arun K Prasad
The primary challenge in developing a gas sensor is achieving high selectivity for the target gas. Most sensor materials respond to multiple gases, making it difficult to discern between various toxic gases. The present study reports the enhancement of the selectivity towards NO2 gas by introducing vanadyl oxygen (OI) vacancies in novel 2D V2O5. The chemical exfoliation process, which is utilized in the present study to synthesize bilayer nanosheets of V2O5, intrinsically generates OI vacancies. The presence of O-vacancy defects, predominantly OI vacancies, in the sample is confirmed using X-ray photoelectron spectroscopy, photoluminescence spectroscopy, and Raman spectroscopy. The bilayer 2D V2O5 showed a highly selective chemiresistive response towards NO2 gas at room temperature unlike normally observed higher temperature sensor response by V2O5, typically above 100 °C. Along with the effect of high surface to volume ratio, the room temperature gas sensing performance by 2D V2O5 stems from the presence of OI vacancy defects and the consequent increase in the surface activity. In addition, the presence of OI vacancies leads to highly selective response to NO2, since NO2 is a highly oxidizing gas with a pair of lone electrons. Hence, the present study is the first to reveal novel bilayer V2O5 sensor with a highly selective response to NO2 at ambient temperature.
{"title":"Highly selective room temperature detection of NO2 enabled by vanadyl oxygen vacancies in novel bilayer V2O5","authors":"Reshma P R, Arun K Prasad","doi":"10.1016/j.talo.2025.100497","DOIUrl":"10.1016/j.talo.2025.100497","url":null,"abstract":"<div><div>The primary challenge in developing a gas sensor is achieving high selectivity for the target gas. Most sensor materials respond to multiple gases, making it difficult to discern between various toxic gases. The present study reports the enhancement of the selectivity towards NO<sub>2</sub> gas by introducing vanadyl oxygen (O<sub>I</sub>) vacancies in novel 2D V<sub>2</sub>O<sub>5</sub>. The chemical exfoliation process, which is utilized in the present study to synthesize bilayer nanosheets of V<sub>2</sub>O<sub>5</sub>, intrinsically generates O<sub>I</sub> vacancies. The presence of O-vacancy defects, predominantly O<sub>I</sub> vacancies, in the sample is confirmed using X-ray photoelectron spectroscopy, photoluminescence spectroscopy, and Raman spectroscopy. The bilayer 2D V<sub>2</sub>O<sub>5</sub> showed a highly selective chemiresistive response towards NO<sub>2</sub> gas at room temperature unlike normally observed higher temperature sensor response by V<sub>2</sub>O<sub>5</sub>, typically above 100 °C. Along with the effect of high surface to volume ratio, the room temperature gas sensing performance by 2D V<sub>2</sub>O<sub>5</sub> stems from the presence of O<sub>I</sub> vacancy defects and the consequent increase in the surface activity. In addition, the presence of O<sub>I</sub> vacancies leads to highly selective response to NO<sub>2</sub>, since NO<sub>2</sub> is a highly oxidizing gas with a pair of lone electrons. Hence, the present study is the first to reveal novel bilayer V<sub>2</sub>O<sub>5</sub> sensor with a highly selective response to NO<sub>2</sub> at ambient temperature.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100497"},"PeriodicalIF":4.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-09DOI: 10.1016/j.talo.2025.100499
Ahmed S. El-tahlawy , Abdullah S. Alawam , Hassan A. Rudayn , Ahmed A. Allam , Rehab Mahmoud , Hany Abd El-Raheem , Waleed Alahmad
The integrity of the global food supply is increasingly compromised by sophisticated fraudulent methods such as adulteration, mislabeling, and substitution. These deceptive practices introduce undisclosed and harmful substances that often evade traditional detection, posing significant risks to public health and consumer trust. This review synthesizes cutting-edge advancements in analytical and digital technologies that are redefining food fraud surveillance. We explore the convergence of molecular diagnostics (e.g., DNA barcoding, clustered regularly interspaced short palindromic repeats (CRISPR), next-generation sequencing (NGS)), high-throughput spectroscopic platforms (e.g., Raman spectroscopy, mass spectrometry), biosensors, and digital innovations (e.g., artificial intelligence (AI), Internet of Things (IoT), blockchain, and lab-on-a-chip (LOC) devices). These integrated approaches enable real-time, proactive detection of fraud across complex and globalized supply chains, offering sensitivity, scalability, and traceability previously unattainable. By framing food fraud as a form of emerging contamination, this work aligns with the paradigm shift toward predictive, data-driven risk assessment in contaminant detection. The review also addresses key translational bottlenecks such as cost, standardization, and interoperability, and proposes a systems-level roadmap to bridge scientific innovation with regulatory and industrial application. This interdisciplinary perspective advocates for a future where food authenticity assurance is intelligent, holistic, and resilient in the face of evolving adulteration threats.
{"title":"Advanced analytical and digital approaches for proactive detection of food fraud as an emerging contaminant threat","authors":"Ahmed S. El-tahlawy , Abdullah S. Alawam , Hassan A. Rudayn , Ahmed A. Allam , Rehab Mahmoud , Hany Abd El-Raheem , Waleed Alahmad","doi":"10.1016/j.talo.2025.100499","DOIUrl":"10.1016/j.talo.2025.100499","url":null,"abstract":"<div><div>The integrity of the global food supply is increasingly compromised by sophisticated fraudulent methods such as adulteration, mislabeling, and substitution. These deceptive practices introduce undisclosed and harmful substances that often evade traditional detection, posing significant risks to public health and consumer trust. This review synthesizes cutting-edge advancements in analytical and digital technologies that are redefining food fraud surveillance. We explore the convergence of molecular diagnostics (e.g., DNA barcoding, clustered regularly interspaced short palindromic repeats (CRISPR), next-generation sequencing (NGS)), high-throughput spectroscopic platforms (e.g., Raman spectroscopy, mass spectrometry), biosensors, and digital innovations (e.g., artificial intelligence (AI), Internet of Things (IoT), blockchain, and lab-on-a-chip (LOC) devices). These integrated approaches enable real-time, proactive detection of fraud across complex and globalized supply chains, offering sensitivity, scalability, and traceability previously unattainable. By framing food fraud as a form of emerging contamination, this work aligns with the paradigm shift toward predictive, data-driven risk assessment in contaminant detection. The review also addresses key translational bottlenecks such as cost, standardization, and interoperability, and proposes a systems-level roadmap to bridge scientific innovation with regulatory and industrial application. This interdisciplinary perspective advocates for a future where food authenticity assurance is intelligent, holistic, and resilient in the face of evolving adulteration threats.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100499"},"PeriodicalIF":4.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-09DOI: 10.1016/j.talo.2025.100500
Namrah Azmi , Sachin Rathod , Bruno Telli Ceccato , Rasmus Kranold Mikkelsen , Jon Otto Fossum
Autofluorescence, an intrinsic property of biological molecules that emit light upon excitation, is gaining significance as a noninvasive technique for studying plant-derived bioactive compounds. This phenomenon enables detection of biomolecules such as chlorophyll, lignin, flavonoids, and alkaloids without requiring external dyes or destructive extraction processes. Intrinsic fluorescence can also aid in detecting and identifying biomolecules that typically require extensive sample preparation (molecule labelling with external fluorophores), eliminating the need for external probes. The present study aims to report the intrinsic autofluorescence in the Moringa Oleifera plant, highlighting its potential to enhance noninvasive bioactive compound identification and quality assessment in plant-based products.
{"title":"Intrinsic fluorescence in Moringa Oleifera seed extract","authors":"Namrah Azmi , Sachin Rathod , Bruno Telli Ceccato , Rasmus Kranold Mikkelsen , Jon Otto Fossum","doi":"10.1016/j.talo.2025.100500","DOIUrl":"10.1016/j.talo.2025.100500","url":null,"abstract":"<div><div>Autofluorescence, an intrinsic property of biological molecules that emit light upon excitation, is gaining significance as a noninvasive technique for studying plant-derived bioactive compounds. This phenomenon enables detection of biomolecules such as chlorophyll, lignin, flavonoids, and alkaloids without requiring external dyes or destructive extraction processes. Intrinsic fluorescence can also aid in detecting and identifying biomolecules that typically require extensive sample preparation (molecule labelling with external fluorophores), eliminating the need for external probes. The present study aims to report the intrinsic autofluorescence in the <em>Moringa Oleifera</em> plant, highlighting its potential to enhance noninvasive bioactive compound identification and quality assessment in plant-based products.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100500"},"PeriodicalIF":4.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As one of the representative layered transition metal dichalcogenides (LTMDC), molybdenum disulfide (MoS₂) has been widely researched as a promising nanoplatform for biomedicine as well as green technologies. The features of high surface-to-volume ratio, tunable bandgap, and good biocompatibility make it a promising candidate for therapeutic strategies, biomedical detection, and environmental applications. Low-dimensional MoS₂ has been widely investigated for drug delivery, bioimaging, photothermal therapy (PTT), photodynamic therapy (PDT), biosensing, and environmental innovations in the past few years, owing to its strong interaction with biomolecules and the cellular microenvironment. The utility of MoS₂ as an alternative to traditional nanomaterials has been assisted by various functionalization strategies to improve solubility, stability, and targeted applications. Moreover, MoS₂-based biosensors have demonstrated remarkable sensitivity for detecting biomolecules, pathogens, and disease-specific biomarkers that enable early and accurate disease diagnosis. This ability is critical to precision medicine, which allows for personalized treatment approaches, real-time health monitoring, and target activation or suppression of pathways based on a patient's biological profile. Additionally, it has become a beacon of environmental application innovation, providing long-term answers to urgent ecological problems. Because of special physicochemical characteristics, contaminants, including gases, organic compounds, heavy metals, and radionuclides, can be effectively removed from the environment, leading to cleaner air and water supplies. Nonetheless, industrial translation of MoS₂ requires the resolution of toxicity, long-term stability, and large-scale synthesis issues. This review aims to comprehensively discuss the recent development, biomedical and environmental applications, challenges, and future perspectives of low-dimensional MoS₂ in the field of next-generation sustainable technology.
{"title":"Unlocking the potential of low-dimensional MoS2 as a smart nanoplatform for environmental technologies, therapeutic strategies, and biomedical sensing","authors":"Smriti Gaba, Mridul Sahu, Nidhi Chauhan, Utkarsh Jain","doi":"10.1016/j.talo.2025.100498","DOIUrl":"10.1016/j.talo.2025.100498","url":null,"abstract":"<div><div>As one of the representative layered transition metal dichalcogenides (LTMDC), molybdenum disulfide (MoS₂) has been widely researched as a promising nanoplatform for biomedicine as well as green technologies. The features of high surface-to-volume ratio, tunable bandgap, and good biocompatibility make it a promising candidate for therapeutic strategies, biomedical detection, and environmental applications. Low-dimensional MoS₂ has been widely investigated for drug delivery, bioimaging, photothermal therapy (PTT), photodynamic therapy (PDT), biosensing, and environmental innovations in the past few years, owing to its strong interaction with biomolecules and the cellular microenvironment. The utility of MoS₂ as an alternative to traditional nanomaterials has been assisted by various functionalization strategies to improve solubility, stability, and targeted applications. Moreover, MoS₂-based biosensors have demonstrated remarkable sensitivity for detecting biomolecules, pathogens, and disease-specific biomarkers that enable early and accurate disease diagnosis. This ability is critical to precision medicine, which allows for personalized treatment approaches, real-time health monitoring, and target activation or suppression of pathways based on a patient's biological profile. Additionally, it has become a beacon of environmental application innovation, providing long-term answers to urgent ecological problems. Because of special physicochemical characteristics, contaminants, including gases, organic compounds, heavy metals, and radionuclides, can be effectively removed from the environment, leading to cleaner air and water supplies. Nonetheless, industrial translation of MoS₂ requires the resolution of toxicity, long-term stability, and large-scale synthesis issues. This review aims to comprehensively discuss the recent development, biomedical and environmental applications, challenges, and future perspectives of low-dimensional MoS₂ in the field of next-generation sustainable technology.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100498"},"PeriodicalIF":4.1,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-05DOI: 10.1016/j.talo.2025.100496
Felix Schmauder, Johannes Brockelt, Kim Brettschneider, Stephan Seifert, Marina Creydt, Markus Fischer
Metabolomics-based approaches are a widely used method for determining the origin of food. The aim of this study was to investigate the suitability of metabolomics analysis using direct analysis in real time mass spectrometry (DART-MS) to ascertain the origin of strawberries. In addition, optimization possibilities and limitations were identified by testing different extraction methods, two different sample delivery systems (Quick-Strip and Dip-it approaches), and running some of the measurements in both positive and negative ionization mode proved to be the most suitable. A total of 207 strawberry samples from six countries were measured and classified using random forest with this optimized approach. The classification accuracy was 63.8%, which shows certain limitations of the approach presented. However, another aim of this study was to protect German strawberries from adulteration with foreign strawberries, and this was achieved with a promising accuracy of 90.3%.
{"title":"DART-MS approaches for metabolomics-based authentication: Challenges in determining the geographical origin of strawberries (Fragaria × ananassa)","authors":"Felix Schmauder, Johannes Brockelt, Kim Brettschneider, Stephan Seifert, Marina Creydt, Markus Fischer","doi":"10.1016/j.talo.2025.100496","DOIUrl":"10.1016/j.talo.2025.100496","url":null,"abstract":"<div><div>Metabolomics-based approaches are a widely used method for determining the origin of food. The aim of this study was to investigate the suitability of metabolomics analysis using direct analysis in real time mass spectrometry (DART-MS) to ascertain the origin of strawberries. In addition, optimization possibilities and limitations were identified by testing different extraction methods, two different sample delivery systems (Quick-Strip and Dip-it approaches), and running some of the measurements in both positive and negative ionization mode proved to be the most suitable. A total of 207 strawberry samples from six countries were measured and classified using random forest with this optimized approach. The classification accuracy was 63.8%, which shows certain limitations of the approach presented. However, another aim of this study was to protect German strawberries from adulteration with foreign strawberries, and this was achieved with a promising accuracy of 90.3%.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100496"},"PeriodicalIF":4.1,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Candida species are opportunistic fungi that can lead to a wide range of infections in humans, ranging from superficial to systemic. The diagnosis and treatment of Candida infections present significant challenges due to the diversity and resistance of these species, as well as the lack of rapid and sensitive detection methods. Microfluidics presents an opportunity to develop quick and highly sensitive diagnostic devices, enabling disease detection with minimal sample volumes. This study aimed to design and fabricate a microfluidic chip for the rapid and sensitive detection of Candida albicans in clinical samples. The dimensions of the chip were approximately 3.5 × 3.5 cm. The master chip was fabricated using soft lithography, and replicated in polydimethylsiloxane (PDMS), a biocompatible and transparent elastomer, which was treated with plasma to activate its surface. The study found that 150 samples (75 %) tested positive for Candida species, with Candida albicans being the most prevalent species at 58 %, followed by other non-albicans Candida species. Additionally, the study revealed that Candida infections were more common among women, young adults, the elderly, and patients with gastrointestinal diseases. The microfluidic chip demonstrated the ability to detect Candida albicans in clinical samples with a low detection limit of 10 CFU/mL. The study concluded that the microfluidic chip is a promising tool for diagnosing Candida infections in point-of-care settings and emphasized that Candida infections represent a common and diverse issue among patients.
{"title":"Design and fabrication of a rapid detection biosensor for Candida albicans diagnosis in a microfluidic platform","authors":"Shekoufeh Moradkhah , Mohaddeseh Larypoor , Abdollah Allahverdi","doi":"10.1016/j.talo.2025.100478","DOIUrl":"10.1016/j.talo.2025.100478","url":null,"abstract":"<div><div>Candida species are opportunistic fungi that can lead to a wide range of infections in humans, ranging from superficial to systemic. The diagnosis and treatment of Candida infections present significant challenges due to the diversity and resistance of these species, as well as the lack of rapid and sensitive detection methods. Microfluidics presents an opportunity to develop quick and highly sensitive diagnostic devices, enabling disease detection with minimal sample volumes. This study aimed to design and fabricate a microfluidic chip for the rapid and sensitive detection of <em>Candida albicans</em> in clinical samples. The dimensions of the chip were approximately 3.5 × 3.5 cm. The master chip was fabricated using soft lithography, and replicated in polydimethylsiloxane (PDMS), a biocompatible and transparent elastomer, which was treated with plasma to activate its surface. The study found that 150 samples (75 %) tested positive for Candida species, with <em>Candida albicans</em> being the most prevalent species at 58 %, followed by other non-albicans Candida species. Additionally, the study revealed that Candida infections were more common among women, young adults, the elderly, and patients with gastrointestinal diseases. The microfluidic chip demonstrated the ability to detect <em>Candida albicans</em> in clinical samples with a low detection limit of 10 CFU/mL. The study concluded that the microfluidic chip is a promising tool for diagnosing Candida infections in point-of-care settings and emphasized that Candida infections represent a common and diverse issue among patients.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100478"},"PeriodicalIF":4.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wearable biosensor systems represent a significant breakthrough in the life sciences, offering real-time monitoring and quantitative assessment of various human health parameters. The escalating demand for continuous and immediate surveillance of both acute and chronic conditions, compounded by constraints in clinical infrastructure, has propelled extensive investigation into wearable biosensors. These systems have emerged as pivotal tools for furnishing physiological insights and facilitating non-invasive monitoring. They monitor various analytes through the analysis of sweat, epidermal, Interstitial fluids, breath and tears, and easily incorporated into flexible substrates ensuring long-term wear and continuous monitoring. However, these biosensors penetrate deeper into the body's fluid networks, providing a systemic approach to continuous monitoring of biomarkers, which is particularly useful for individuals with metabolic disorders. This comprehensive review explores recent trends and diverse applications of wearable biosensors. These applications empower individuals to dynamically assess their well-being, enabling informed healthcare decisions tailored to individual needs. The convergence of these approaches in wearable biosensors highlights a comprehensive approach to understand individual health. This integration holds the promise of transforming healthcare practice by providing continuous observance and remote monitoring. The result has been a paradigm shift towards active, personalized health care, leading to improved health outcomes and improved quality of life
{"title":"Wearable biosensors in modern healthcare: Emerging trends and practical applications","authors":"Maumita Das Mukherjee , Priyanshi Gupta , Vanshika Kumari , Ishika Rana , Drishti Jindal , Navya Sagar , Jay Singh , Chetna Dhand","doi":"10.1016/j.talo.2025.100486","DOIUrl":"10.1016/j.talo.2025.100486","url":null,"abstract":"<div><div>Wearable biosensor systems represent a significant breakthrough in the life sciences, offering real-time monitoring and quantitative assessment of various human health parameters. The escalating demand for continuous and immediate surveillance of both acute and chronic conditions, compounded by constraints in clinical infrastructure, has propelled extensive investigation into wearable biosensors. These systems have emerged as pivotal tools for furnishing physiological insights and facilitating non-invasive monitoring. They monitor various analytes through the analysis of sweat, epidermal, Interstitial fluids, breath and tears, and easily incorporated into flexible substrates ensuring long-term wear and continuous monitoring. However, these biosensors penetrate deeper into the body's fluid networks, providing a systemic approach to continuous monitoring of biomarkers, which is particularly useful for individuals with metabolic disorders. This comprehensive review explores recent trends and diverse applications of wearable biosensors. These applications empower individuals to dynamically assess their well-being, enabling informed healthcare decisions tailored to individual needs. The convergence of these approaches in wearable biosensors highlights a comprehensive approach to understand individual health. This integration holds the promise of transforming healthcare practice by providing continuous observance and remote monitoring. The result has been a paradigm shift towards active, personalized health care, leading to improved health outcomes and improved quality of life</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100486"},"PeriodicalIF":4.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-29DOI: 10.1016/j.talo.2025.100494
Anna Lisa Gilioli , Alessio Sacco , Andrea Mario Giovannozzi , Simone Giacosa , Antonella Bosso , Loretta Panero , Silvia Raffaela Barera , Stefano Messina , Marco Lagori , Silvia Motta , Massimo Guaita , Ettore Vittone , Andrea Mario Rossi
Lactic acid is mainly produced during the process of malolactic fermentation and evolution of its concentration is associated with the wine stabilization process and the quality of the final product. The quantitative analysis of lactic acid is carried out offline in the laboratory using various analytical techniques, the most used being high performance liquid chromatography (HPLC). Because of this, there is a clear demand in the winemaking community for analytical tools that allow real-time, fast and inexpensive quantification of lactic acid. An approach using Raman spectroscopy has positioned itself as a feasible alternative in this regard. The primary goal of this work is therefore to monitor the concentration of lactic acid (which changes rapidly during the malolactic fermentation process) in the analysed samples, specifically, Nebbiolo wine samples for making the Barolo wine. The collected Raman spectra using a portable Raman apparatus are processed using an algorithm that applies Partial Least Squares (PLS) regression to determine the lactic acid concentration for each sample. It proves to be a precise and reliable method that leads to the determination of a predictive model characterised by (on the validation set), (on the test set) and RMSE of the lactic acid concentration predicted by the model of 0.22 g/l (on the validation set) and 0.11 g/l (on the test set) respectively. This approach produces results comparable to those obtained via HPLC. Moreover, unlike the latter, it allows rapid and easy monitoring of the lactic acid concentration during fermentation directly in the winery.
{"title":"Raman spectroscopy as a rapid tool for monitoring lactic acid concentration during wine malolactic fermentation directly in the winery","authors":"Anna Lisa Gilioli , Alessio Sacco , Andrea Mario Giovannozzi , Simone Giacosa , Antonella Bosso , Loretta Panero , Silvia Raffaela Barera , Stefano Messina , Marco Lagori , Silvia Motta , Massimo Guaita , Ettore Vittone , Andrea Mario Rossi","doi":"10.1016/j.talo.2025.100494","DOIUrl":"10.1016/j.talo.2025.100494","url":null,"abstract":"<div><div>Lactic acid is mainly produced during the process of malolactic fermentation and evolution of its concentration is associated with the wine stabilization process and the quality of the final product. The quantitative analysis of lactic acid is carried out offline in the laboratory using various analytical techniques, the most used being high performance liquid chromatography (HPLC). Because of this, there is a clear demand in the winemaking community for analytical tools that allow real-time, fast and inexpensive quantification of lactic acid. An approach using Raman spectroscopy has positioned itself as a feasible alternative in this regard. The primary goal of this work is therefore to monitor the concentration of lactic acid (which changes rapidly during the malolactic fermentation process) in the analysed samples, specifically, Nebbiolo wine samples for making the Barolo wine. The collected Raman spectra using a portable Raman apparatus are processed using an algorithm that applies Partial Least Squares (PLS) regression to determine the lactic acid concentration for each sample. It proves to be a precise and reliable method that leads to the determination of a predictive model characterised by <span><math><mrow><msup><mrow><mi>R</mi></mrow><mn>2</mn></msup><mo>=</mo><mn>0.76</mn></mrow></math></span> (on the validation set), <span><math><mrow><msup><mrow><mi>R</mi></mrow><mn>2</mn></msup><mi>░</mi><mo>=</mo><mi>░</mi><mn>0.94</mn></mrow></math></span> (on the test set) and RMSE of the lactic acid concentration predicted by the model of 0.22 g/l (on the validation set) and 0.11 g/l (on the test set) respectively. This approach produces results comparable to those obtained via HPLC. Moreover, unlike the latter, it allows rapid and easy monitoring of the lactic acid concentration during fermentation directly in the winery.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100494"},"PeriodicalIF":4.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号