Talanta Open最新文献_第10页

Graphene oxide and molecularly imprinted polymer-based sensor for the electrochemical detection of benzene: A novel tactic in early diagnosis of lung cancer 基于氧化石墨烯和分子印迹聚合物的苯电化学检测传感器：肺癌早期诊断的新策略

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-06-25 DOI: 10.1016/j.talo.2025.100506

Warren Rosario , Devesh K. Avasthi , Nidhi Chauhan

Early cancer detection significantly enhances survival rates, making widespread diagnostic screening essential. However, the high cost and limited accessibility of diagnostic tools pose major challenges. Nanomaterial-based sensors offer a promising solution due to their portability, user-friendly design, and cost-effective manufacturing. Volatile organic compounds (VOCs) emitted by the human body are closely linked to the presence of life-threatening diseases, with benzene emerging as a significant biomarker in the breath and blood of individuals at risk of developing lung cancer. Leveraging this correlation, we have developed a sensor capable of detecting benzene with high reliability. This study details the fabrication of an electrochemical sensor on a screen-printed electrode (SPE), modified with graphene oxide (GO) followed by molecularly imprinted polymer (MIP). SPE promotes the development of a miniaturized and user-friendly sensing platform. The incorporation of GO is responsible for improving the sensor's response due to its excellent electrochemical properties. The MIP is synthesized using benzene as a template, which creates imprints of benzene on the polymer. Integration of this MIP significantly improves the sensor’s selectivity. This approach addresses the key limitation of low selectivity in VOC sensors for medical applications. The sensor demonstrated a broad linear range of 0.1–1000 ppb with a sensitivity of 15.5 µA/ppb and exhibited excellent selectivity toward benzene. By enhancing both sensitivity and selectivity, the developed sensor offers a promising solution toward effective, accessible, and early lung cancer diagnosis.

早期癌症检测可显著提高生存率，因此广泛的诊断筛查必不可少。然而，诊断工具的高成本和有限的可及性构成了主要挑战。基于纳米材料的传感器由于其便携性、用户友好的设计和具有成本效益的制造而提供了一个有前途的解决方案。人体排放的挥发性有机化合物（VOCs）与危及生命的疾病密切相关，苯成为有患肺癌风险个体呼吸和血液中的重要生物标志物。利用这种相关性，我们开发了一种能够高可靠性检测苯的传感器。本研究详细介绍了在丝网印刷电极（SPE）上制造电化学传感器的过程，该电极用氧化石墨烯（GO）和分子印迹聚合物（MIP）修饰。SPE促进了小型化和用户友好型传感平台的发展。氧化石墨烯的加入是负责改善传感器的响应，由于其优异的电化学性能。MIP是用苯作为模板合成的，它在聚合物上产生苯的印迹。该MIP的集成显著提高了传感器的选择性。这种方法解决了用于医疗应用的VOC传感器低选择性的关键限制。该传感器具有0.1-1000 ppb的宽线性范围，灵敏度为15.5µa /ppb，对苯具有优异的选择性。通过提高灵敏度和选择性，所开发的传感器为有效、方便和早期肺癌诊断提供了一个有希望的解决方案。

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引用次数: 0

Molecularly imprinted polymer-based sensors: Design and advances in the analysis of DNA and protein 分子印迹聚合物传感器：DNA和蛋白质分析的设计和进展

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-06-25 DOI: 10.1016/j.talo.2025.100507

Arzum Erdem, Huseyin Senturk, Mehmet Karakus

Molecularly imprinted polymers (MIPs) have emerged in recent years as highly promising materials for sensor design, owing to their high selectivity, stability, and reusability toward target analytes. This review specifically focuses on MIP-based sensor applications aimed at detecting critically important biomolecules such as proteins and DNA, which play essential roles especially in the biomedical field. Although antibody-based immunoassays for protein detection and PCR-based methods for DNA analysis provide high specificity and sensitivity, these conventional approaches have significant limitations, including high costs, limited stability, complex instrumentation, and the necessity of highly skilled personnel. MIPs have recently gained attention as synthetic recognition elements capable of overcoming these limitations. The rationale behind jointly addressing protein and DNA analysis lies primarily in the shared challenges presented by these biomolecules, such as molecular size, structural complexity, and specificity of binding. Furthermore, similar analytical approaches and transduction mechanisms employed in the sensor designs for these two groups allow for a more comprehensive and integrated evaluation. This review thoroughly examines MIP structures integrated into electrochemical, optical, quartz crystal microbalance (QCM), and other sensor platforms. Current limitations such as heterogeneity of binding sites and incomplete removal of template molecules are critically discussed, alongside proposed solutions like incorporation of nanomaterials, computational modeling, and novel polymerization strategies. In conclusion, this review provides an extensive evaluation of recent advances in protein and DNA detection using MIP-based sensors, clearly outlining the current state, encountered challenges, and future perspectives within the field.

分子印迹聚合物（MIPs）由于其对目标分析物的高选择性、稳定性和可重复使用性，近年来成为传感器设计中非常有前途的材料。本文综述了基于mip的传感器在检测重要生物分子（如蛋白质和DNA）方面的应用，这些分子在生物医学领域发挥着重要作用。尽管基于抗体的蛋白质检测免疫测定和基于pcr的DNA分析方法具有高特异性和敏感性，但这些传统方法具有显著的局限性，包括成本高、稳定性有限、仪器复杂以及需要高技能人员。最近，MIPs作为能够克服这些限制的合成识别元素而受到关注。联合处理蛋白质和DNA分析的基本原理主要在于这些生物分子所面临的共同挑战，如分子大小、结构复杂性和结合的特异性。此外，在这两组传感器设计中采用了类似的分析方法和转导机制，可以进行更全面和综合的评估。本文综述了集成在电化学、光学、石英晶体微天平（QCM）和其他传感器平台上的MIP结构。目前的限制，如结合位点的异质性和模板分子的不完全去除进行了批判性的讨论，以及提出的解决方案，如纳米材料的掺入，计算建模，和新的聚合策略。总之，本综述对基于mip传感器的蛋白质和DNA检测的最新进展进行了广泛的评估，清楚地概述了该领域的现状、遇到的挑战和未来前景。

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引用次数: 0

3D printed hardware for automation of proteomics sample preparation at the Meso-Scale 用于中观尺度蛋白质组学样品制备自动化的3D打印硬件

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-06-19 DOI: 10.1016/j.talo.2025.100505

Sadie R. Schultz , Matthew M. Champion

Mass spectrometry-based proteomics is the dominant method for measuring peptides and proteins from complex mixtures. In bottom-up approaches, proteins are digested or proteolyzed prior to LC-MS/MS analysis. Peptides are fragmented, and proteins are inferred via peptide spectral matching (PSM). The throughput of this process is surprisingly low; a proteomics core facility might analyse <20 samples/day per instrument using UHPLC-MS/MS. Because of this, automation in proteomics is rare, and virtually all preparation is performed by hand. We developed 3D printed hardware and automated sample preparation modules for a lower-cost Andrew Alliance pipetting robot. The robot operates on simple principles, using traditional pipettes and follows protocols closely resembling manual preparation. Here, we present modular protocols for the major techniques in proteomics preparation: in-solution and S-Trap digestion; Tip and solid-phase extraction (SPE) based desalting. Both approaches yield dense protein identifications from complex proteomes. Automated samples had high reproducibility: ∼60 % of proteins identified from in-solution and S-Trap digested samples had a measured CV of ≤20 %. In contrast, 52 % of in-solution digested and 63 % of S-Trap digested of proteins identified from manually prepared samples had CVs ≤20 %. Automated sample digestion and tip-based desalting had reduced ≅ 70 % and 40 % quantitative yield respectively compared to manual preparation according to the protein label-free quantification (LFQ). Increasing injection amount to normalize the yield restored protein and peptide identifications which demonstrates the differences between manual and automated methods were predominantly due to reduced recovery. Overall, automation of bottom-up proteomics sample preparation at the meso‑scale offers increased reproducibility in non sample-limited applications.

基于质谱的蛋白质组学是测量复杂混合物中多肽和蛋白质的主要方法。在自下而上的方法中，蛋白质在LC-MS/MS分析之前被消化或蛋白水解。肽是碎片化的，通过肽谱匹配（PSM）推断出蛋白质。这个过程的吞吐量低得惊人；蛋白质组学核心设备可能使用UHPLC-MS/MS每天分析20个样品。正因为如此，蛋白质组学的自动化是罕见的，几乎所有的准备工作都是手工完成的。我们开发了3D打印硬件和自动化样品制备模块的低成本安德鲁联盟移液机器人。机器人的操作原理很简单，使用传统的移液器，并遵循与人工准备非常相似的规程。在这里，我们提出了蛋白质组学制备的主要技术的模块化方案：溶液中和S-Trap消化；尖端和固相萃取（SPE）脱盐。这两种方法都能从复杂的蛋白质组中获得密集的蛋白质鉴定。自动化样品具有高重复性：从溶液中和S-Trap消化样品中鉴定的蛋白质中，约60%的测量CV≤20%。相比之下，人工制备样品中鉴定的蛋白质，52%的溶液消化和63%的S-Trap消化的CVs≤20%。根据蛋白质无标签定量（LFQ），与人工制备相比，自动样品消化和基于尖端的脱盐分别降低了70%和40%的定量收率。增加注射量以使产量恢复正常，这表明手动和自动化方法之间的差异主要是由于回收率降低。总体而言，自动化自下而上的蛋白质组学样品制备在中尺度提供了增加的再现性，在非样品限制的应用。

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引用次数: 0

Impact of atmospheric pressure DBD plasma treatment on the biodegradability, hydrophilicity, and mechanical properties of PVA/PEG/Chitosan/HA nanofibers for bone tissue engineering 常压DBD等离子体处理对骨组织工程用PVA/PEG/壳聚糖/HA纳米纤维的生物降解性、亲水性和力学性能的影响

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-06-18 DOI: 10.1016/j.talo.2025.100501

Hartatiek , M.I. Wuriantika , Yudyanto , A. Taufiq , M. Diantoro , Y. Yusuf , M. Taufik , J.F. Fatriansyah

The effectiveness of scaffolds in supporting bone tissue regeneration is highly dependent on surface modification. Atmospheric pressure dielectric barrier (DBD) plasma treatment offers a practical method that offers simplicity, time efficiency, cost effectiveness, and reliable results. In this study, nanofiber scaffolds composed of PVA, PEG, chitosan, and hydroxyapatite were fabricated using electrospinning and treated with DBD plasma at varying distances. Optical emission spectroscopy (OES) confirmed the presence of reactive species such as OH radicals, nitrogen (N₂), and nitrogen ions (N₂⁺). These species caused changes in surface morphology, including an increase in fiber diameter. These surface morphological modifications were associated with mechanical properties and hydrophilicity. Furthermore, scaffolds treated at a distance of 1.5 mm showed a degradation rate of up to 70 % after three weeks. The results highlight the potential of DBD plasma treatment to optimize the performance of bone tissue engineering scaffolds.

支架支持骨组织再生的有效性高度依赖于表面修饰。大气压介质阻挡（DBD）等离子体处理提供了一种实用的方法，具有简单、省时、成本效益和可靠的结果。在本研究中，采用静电纺丝法制备了聚乙烯醇、聚乙二醇、壳聚糖和羟基磷灰石组成的纳米纤维支架，并用DBD等离子体在不同距离上处理。光学发射光谱（OES）证实了OH自由基、氮（N2）和氮离子（N2+）等活性物质的存在。这些物种引起了表面形态的变化，包括纤维直径的增加。这些表面形态的改变与机械性能和亲水性有关。此外，在1.5 mm距离处处理的支架在三周后的降解率高达70%。结果表明，DBD等离子体处理在优化骨组织工程支架性能方面具有潜力。

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引用次数: 0

Structural isomeric aromatic diamine linkers influenced electrocatalysis of AuNPs: Efficient assessment of hydrazine in environmental water samples 结构异构体芳香二胺连接物影响AuNPs的电催化作用：环境水样中肼的有效评价

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-06-14 DOI: 10.1016/j.talo.2025.100503

Karmegam Muthukrishnan , N.S.K. Gowthaman , Bharathi Sinduja , Mathur Gopalakrishnan Sethuraman

This electrochemical research explores the conductive behaviour and sensor functionality of citrate-stabilized gold nanoparticles (AuNPs) constructed on a glassy carbon (GC) electrode. Aromatic diamines (ArDAs) with different structural isomeric forms were used as efficient linkers for the attachment of AuNPs, with particular attention given to the detection of hydrazine (HYZ). Careful analysis of three distinct ArDAs, ortho-phenylenediamine (OPD), meta-phenylenediamine (MPD), and para-phenylenediamine (PPD), revealed the formation of self-assembled monolayers (SAMs) of ArDAs on the GC electrode surface. The growth kinetics of ArDA SAMs on GC surfaces were examined using ATR-FT-IR, focusing on the -NH₂ bending vibration region (1592–1631 cm^-1), and the Langmuir adsorption kinetics described the SAM formation, specifically, faster and easier formation of PPD-SAM than OPD and MPD. The PPD SAM on the electrode surface was found to be more compact than OPD and MPD, according to the multi-method analysis. The synthesized AuNPs were anchored on the electrode surface through the free amine groups present in the SAMs and confirmed through scanning electron microscopy, X-ray photoelectron spectroscopy, and cyclic voltammetry. The AuNPs/PPD/GC electrode exhibited superior sensitivity (562 µA mM^-1 cm^-2) and a low detection limit (30 nM) towards HYZ determination. Further, the practicability of the proposed sensor is evaluated by detecting HYZ in environmental water samples and validating the results with the ultra-high-performance liquid chromatography method.

这项电化学研究探索了构建在玻碳（GC）电极上的柠檬酸稳定金纳米颗粒（AuNPs）的导电行为和传感器功能。采用不同结构异构体形式的芳香二胺（ArDAs）作为连接AuNPs的有效连接剂，特别关注了联氨（HYZ）的检测。仔细分析三种不同的arda，邻苯二胺（OPD），间苯二胺（MPD）和对苯二胺（PPD），发现在GC电极表面形成了arda的自组装单层（sam）。利用ATR-FT-IR研究了ArDA SAMs在GC表面的生长动力学，重点研究了-NH2弯曲振动区（1592-1631 cm-1）， Langmuir吸附动力学描述了SAM的形成，特别是PPD-SAM比OPD和MPD更快、更容易形成。通过多方法分析发现，电极表面的PPD SAM比OPD和MPD更致密。合成的AuNPs通过SAMs中的游离胺基固定在电极表面，并通过扫描电镜、x射线光电子能谱和循环伏安法进行确认。AuNPs/PPD/GC电极对HYZ的检测灵敏度为562µA mM-1 cm-2，检出限较低（30 nM）。此外，通过对环境水样中的HYZ进行检测，并采用超高效液相色谱法对结果进行验证，评价了该传感器的实用性。

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引用次数: 0

Plasmonic and quantitative lateral flow assay for grayscale imaging of neutralizing antibodies SARS-CoV-2 中和抗体SARS-CoV-2灰度成像的等离子体和定量横向流动试验

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-06-14 DOI: 10.1016/j.talo.2025.100504

Karla R. Castro , Isabela A. Mattioli , Graziela C. Sedenho , Thiago Bertaglia , Antônio F.A. Araújo , Manoel J.A. Lima , Beatriz G.R. da Silva , Mona N. Oliveira , Iris Todeschini , Phelipe M. Vitale , Erika R. Manuli , Geovana M. Pereira , Suzete C. Ferreira , Ester C. Sabino , Emanuel Carrilho , Frank N. Crespilho

Lateral flow immunoassays (LFIAs) have modernized decentralized mass testing and significantly impacted the healthcare system. It provides a rapid and accessible method for serological testing, which is an important tool for screening patients with past viral phases by detecting antibodies. Here, the development of a quantitative LFIA is presented, in which the receptor-binding domain of Spike protein-specific antibody (anti-S-RBD) in serum samples from previously infected with SARS-CoV-2 patients form an immune complex with the RBD protein immobilized on gold nanoparticles. For quantitative measurements, a methodology was developed based on the plasmonic behavior of gold nanoparticles and using a digital camera that processes images of the test lines in a closed chamber using the CYMK color system. The limits of detection and quantification were 0.22 and 0.70 µg mL^–1, respectively. The proposed device has a manufacturing cost of less than US$ 1 from synthesizing the bioconjugates, assembly of the LFIA strips, and 3D printing of the cassettes. The platform could detect anti-S-RBD antibodies in human serum samples and can be particularly useful in strategies for monitoring IgG levels in the population.

侧流免疫测定法（LFIAs）使分散的大规模检测现代化，并对医疗保健系统产生了重大影响。它提供了一种快速、方便的血清学检测方法，是通过检测抗体筛选过去病毒期患者的重要工具。本文提出了一种定量LFIA的发展，其中先前感染SARS-CoV-2的患者血清样品中的Spike蛋白特异性抗体（抗s -RBD）的受体结合域与固定在金纳米颗粒上的RBD蛋白形成免疫复合物。对于定量测量，开发了一种基于金纳米粒子等离子体行为的方法，并使用数码相机在封闭的腔室中使用CYMK颜色系统处理测试线的图像。检测限为0.22µg mL-1，定量限为0.70µg mL-1。该装置的制造成本低于1美元，包括合成生物偶联物、组装LFIA条带和3D打印卡带。该平台可以检测人血清样本中的抗s - rbd抗体，在监测人群中IgG水平的策略中特别有用。

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引用次数: 0

Label-free masterful spectrofluorimetric systems for minuscule tracking of natural antioxidant vincamine in the pharmaceutical, environmental, and biological samples: Comparative greenness, whiteness, and blueness appraisal 无标签熟练的荧光光谱系统，用于制药，环境和生物样品中天然抗氧化剂长春胺的微小跟踪：比较绿色，白色和蓝色评估

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-06-13 DOI: 10.1016/j.talo.2025.100502

Eman Yosrey , Heba Elmansi , Shereen Shalan , Jenny Jeehan Nasr

In this work, we present two label-free spectrofluorimetric systems for the swift quantification of vincamine (VN) in various matrices without the need for knotty fluorescent dyes, drastic conditions, or expensive instrumentation, which are key merits of the quantifying procedures. The first method (Method I) relied on the direct mix-and-read assay for exploiting the intrinsic fluorescence of VN, adopting λ_ex/em of 272/334 nm using water as a diluting solvent. The method fitted linearity across the concentrations of 1.00 – 10.00 µg mL⁻¹. In the second method (Method II), upon turning off the photoinduced electron transfer (PET) of tertiary amine in the piperidine moiety to the indole scaffold using 0.1 M hydrochloric acid, the fluorescent signal was augmented up to 158 %. This commendable boost in the fluorescent sensitivity allowed the minute detection of VN in spiked human plasma with a standard deviation not exceeding 1.30. Using the acid-blocked PET-based probe, the fluorescent signal was linearly correlated with VN concentration across 0.25 – 6.00 µg mL⁻¹ using λ_ex/em of 277/323 nm. These streamlined procedures proficiently evaluated VN in both the sole and co-formulated capsules without any significant interference from capsule filler material or the co-formulated medication “piracetam”. In addition, the two submitted procedures were successfully executed to check VN through weight variation testing in the Brain-ox® capsules with good acceptance values. The constructed platforms ensemble estimated VN in the environmental samples (tap and river water) with acceptable percentage recoveries and standard deviations (0.95 – 1.65). The procedures' green, white, and blue fitness were appraised through a plethora of computational tools, including GAPI, AGREE, AGREEprep, the RGB 12 algorithm, and BAGI. The evaluation showed that the proposed works effectively strike a compromise between sustainability and functionality postulates. This highlighted that designed approaches are perfectly suited as ecological frameworks for minuscule assaying of VN in crucial pharmaceutical, biological, and environmental samples.

在这项工作中，我们提出了两种无标记荧光光谱法系统，用于快速定量各种基质中的长春胺（VN），而不需要复杂的荧光染料，苛刻的条件或昂贵的仪器，这是定量程序的关键优点。第一种方法（方法一）采用直接混合-读取法，以水为稀释溶剂，λex/em为272/334 nm，利用VN的本征荧光。该方法在1.00 - 10.00µg mL−¹浓度范围内线性拟合。在第二种方法（方法二）中，使用0.1 M盐酸关闭哌啶部分叔胺到吲哚支架的光致电子转移（PET）后，荧光信号增强到158%。这种值得称赞的荧光灵敏度的提高使得在加药的人血浆中微小地检测到VN，标准偏差不超过1.30。使用酸阻断pet探针，在λex/em为277/323 nm的范围内，荧光信号与VN浓度在0.25 ~ 6.00µg mL−¹范围内呈线性相关。这些简化的程序熟练地评估了单独和共配制胶囊中的VN，而不受胶囊填充材料或共配制药物“吡拉西坦”的任何显著干扰。此外，提交的两个程序成功执行，通过在Brain-ox®胶囊中进行重量变化测试来检查VN，具有良好的接受值。构建的平台集合估算了环境样品（自来水和河水）中的VN，回收率和标准偏差（0.95 - 1.65）可接受。程序的绿色、白色和蓝色适应度通过大量的计算工具进行评估，包括GAPI、AGREE、AGREEprep、RGB 12算法和BAGI。评估表明，提议的作品有效地在可持续性和功能性假设之间达成妥协。这突出表明，设计的方法非常适合作为在关键的制药、生物和环境样品中微量测定VN的生态框架。

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引用次数: 0

Highly selective room temperature detection of NO2 enabled by vanadyl oxygen vacancies in novel bilayer V2O5 利用新型双分子层V2O5中钒氧空位实现NO2的高选择性室温检测

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-06-09 DOI: 10.1016/j.talo.2025.100497

Reshma P R, Arun K Prasad

The primary challenge in developing a gas sensor is achieving high selectivity for the target gas. Most sensor materials respond to multiple gases, making it difficult to discern between various toxic gases. The present study reports the enhancement of the selectivity towards NO₂ gas by introducing vanadyl oxygen (O_I) vacancies in novel 2D V₂O₅. The chemical exfoliation process, which is utilized in the present study to synthesize bilayer nanosheets of V₂O₅, intrinsically generates O_I vacancies. The presence of O-vacancy defects, predominantly O_I vacancies, in the sample is confirmed using X-ray photoelectron spectroscopy, photoluminescence spectroscopy, and Raman spectroscopy. The bilayer 2D V₂O₅ showed a highly selective chemiresistive response towards NO₂ gas at room temperature unlike normally observed higher temperature sensor response by V₂O₅, typically above 100 °C. Along with the effect of high surface to volume ratio, the room temperature gas sensing performance by 2D V₂O₅ stems from the presence of O_I vacancy defects and the consequent increase in the surface activity. In addition, the presence of O_I vacancies leads to highly selective response to NO₂, since NO₂ is a highly oxidizing gas with a pair of lone electrons. Hence, the present study is the first to reveal novel bilayer V₂O₅ sensor with a highly selective response to NO₂ at ambient temperature.

开发气体传感器的主要挑战是实现对目标气体的高选择性。大多数传感器材料对多种气体有反应，因此很难区分各种有毒气体。本文报道了在新型2D V2O5中引入vanadyl氧（OI）空位，提高了对NO2气体的选择性。本研究利用化学剥离工艺合成V2O5双层纳米片，本质上产生OI空位。利用x射线光电子能谱、光致发光能谱和拉曼能谱证实了样品中存在o空位缺陷，主要是OI空位。在室温下，双层二维V2O5对NO2气体表现出高度选择性的化学反应，这与V2O5通常在100°C以上的高温传感器所观察到的反应不同。在高表面体积比的作用下，二维V2O5的室温气敏性能源于OI空位缺陷的存在以及随之而来的表面活性的提高。此外，OI空位的存在导致对NO2的高度选择性反应，因为NO2是一种具有一对孤电子的高氧化性气体。因此，本研究首次揭示了在环境温度下对NO2具有高度选择性响应的新型双层V2O5传感器。

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引用次数: 0

Advanced analytical and digital approaches for proactive detection of food fraud as an emerging contaminant threat 先进的分析和数字方法，主动检测食品欺诈作为一种新兴的污染物威胁

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-06-09 DOI: 10.1016/j.talo.2025.100499

Ahmed S. El-tahlawy , Abdullah S. Alawam , Hassan A. Rudayn , Ahmed A. Allam , Rehab Mahmoud , Hany Abd El-Raheem , Waleed Alahmad

The integrity of the global food supply is increasingly compromised by sophisticated fraudulent methods such as adulteration, mislabeling, and substitution. These deceptive practices introduce undisclosed and harmful substances that often evade traditional detection, posing significant risks to public health and consumer trust. This review synthesizes cutting-edge advancements in analytical and digital technologies that are redefining food fraud surveillance. We explore the convergence of molecular diagnostics (e.g., DNA barcoding, clustered regularly interspaced short palindromic repeats (CRISPR), next-generation sequencing (NGS)), high-throughput spectroscopic platforms (e.g., Raman spectroscopy, mass spectrometry), biosensors, and digital innovations (e.g., artificial intelligence (AI), Internet of Things (IoT), blockchain, and lab-on-a-chip (LOC) devices). These integrated approaches enable real-time, proactive detection of fraud across complex and globalized supply chains, offering sensitivity, scalability, and traceability previously unattainable. By framing food fraud as a form of emerging contamination, this work aligns with the paradigm shift toward predictive, data-driven risk assessment in contaminant detection. The review also addresses key translational bottlenecks such as cost, standardization, and interoperability, and proposes a systems-level roadmap to bridge scientific innovation with regulatory and industrial application. This interdisciplinary perspective advocates for a future where food authenticity assurance is intelligent, holistic, and resilient in the face of evolving adulteration threats.

全球食品供应的完整性日益受到掺假、贴错标签和替代等复杂欺诈手段的损害。这些欺骗性做法引入了未披露的有害物质，这些物质往往能逃避传统检测，对公众健康和消费者信任构成重大风险。本综述综合了分析和数字技术的前沿进展，这些技术正在重新定义食品欺诈监测。我们探索分子诊断（例如，DNA条形码，聚集定期间隔短回文重复序列（CRISPR），下一代测序（NGS）），高通量光谱平台（例如，拉曼光谱，质谱），生物传感器和数字创新（例如，人工智能（AI），物联网（IoT），区块链和芯片实验室（LOC）设备）的融合。这些集成的方法能够实时、主动地检测复杂和全球化供应链中的欺诈行为，提供以前无法实现的灵敏度、可扩展性和可追溯性。通过将食品欺诈视为一种新出现的污染形式，这项工作与污染物检测中向预测性、数据驱动的风险评估的范式转变保持一致。该综述还解决了关键的转化瓶颈，如成本、标准化和互操作性，并提出了一个系统级路线图，将科学创新与监管和工业应用联系起来。这种跨学科的观点提倡未来食品真实性保证是智能的，整体的，面对不断变化的掺假威胁的弹性。

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引用次数: 0

Intrinsic fluorescence in Moringa Oleifera seed extract 辣木籽提取物的本征荧光

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-06-09 DOI: 10.1016/j.talo.2025.100500

Namrah Azmi , Sachin Rathod , Bruno Telli Ceccato , Rasmus Kranold Mikkelsen , Jon Otto Fossum

Autofluorescence, an intrinsic property of biological molecules that emit light upon excitation, is gaining significance as a noninvasive technique for studying plant-derived bioactive compounds. This phenomenon enables detection of biomolecules such as chlorophyll, lignin, flavonoids, and alkaloids without requiring external dyes or destructive extraction processes. Intrinsic fluorescence can also aid in detecting and identifying biomolecules that typically require extensive sample preparation (molecule labelling with external fluorophores), eliminating the need for external probes. The present study aims to report the intrinsic autofluorescence in the Moringa Oleifera plant, highlighting its potential to enhance noninvasive bioactive compound identification and quality assessment in plant-based products.

自体荧光是一种生物分子在激发时发光的固有特性，它作为一种非侵入性技术在研究植物源性生物活性化合物方面越来越重要。这种现象可以检测生物分子，如叶绿素、木质素、类黄酮和生物碱，而不需要外部染料或破坏性的提取过程。本征荧光还可以帮助检测和识别通常需要大量样品制备（用外部荧光团标记分子）的生物分子，从而消除了对外部探针的需要。本研究旨在报道辣木（Moringa Oleifera）植物的固有荧光，强调其在植物性产品的无创生物活性化合物鉴定和质量评价方面的潜力。

引用次数: 0