Pub Date : 2025-12-01Epub Date: 2025-11-07DOI: 10.1016/j.talo.2025.100592
Svenja Nathalie Weber , Nadin Ulrich , Jana Mühlenberg , Nils Engler , Michael Nelles
A fast and efficient analytical method for the quantification of antibiotic residues in poultry manure based on ultrasonic assisted extraction (UAE) and QuEChERS cleanup followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. It was successfully validated for amoxicillin, cefaclor, ciprofloxacin, clarithromycin, erythromycin, lincomycin, metronidazole and ofloxacin in terms of linearity, detection and quantification limit, recovery, intra-day and inter-day precision and matrix effect. The overall method performance showed good linearity and recoveries ranging between 80.6 % and 138.1 % with sufficient limits of quantification for the targeted analytes. The method was applied to eleven poultry manure samples. Lincomycin and ciprofloxacin were detected in eight of eleven samples. The results show that the developed fast and efficient method can be employed to analyze poultry manure samples. In addition, antibiotic residues can be found regularly in poultry manure samples. When used in biogas plants or as fertilizer, they can pose a risk to human health and the environment. The method also includes cefaclor, an antibiotic that has, to the best of our knowledge, not yet been analysed in poultry manure.
{"title":"Determination of veterinary antibiotics in German poultry manure by ultrasonic assisted extraction and QuEChERS coupled with LC-MS/MS","authors":"Svenja Nathalie Weber , Nadin Ulrich , Jana Mühlenberg , Nils Engler , Michael Nelles","doi":"10.1016/j.talo.2025.100592","DOIUrl":"10.1016/j.talo.2025.100592","url":null,"abstract":"<div><div>A fast and efficient analytical method for the quantification of antibiotic residues in poultry manure based on ultrasonic assisted extraction (UAE) and QuEChERS cleanup followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. It was successfully validated for amoxicillin, cefaclor, ciprofloxacin, clarithromycin, erythromycin, lincomycin, metronidazole and ofloxacin in terms of linearity, detection and quantification limit, recovery, intra-day and inter-day precision and matrix effect. The overall method performance showed good linearity and recoveries ranging between 80.6 % and 138.1 % with sufficient limits of quantification for the targeted analytes. The method was applied to eleven poultry manure samples. Lincomycin and ciprofloxacin were detected in eight of eleven samples. The results show that the developed fast and efficient method can be employed to analyze poultry manure samples. In addition, antibiotic residues can be found regularly in poultry manure samples. When used in biogas plants or as fertilizer, they can pose a risk to human health and the environment. The method also includes cefaclor, an antibiotic that has, to the best of our knowledge, not yet been analysed in poultry manure.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100592"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145614589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-09-15DOI: 10.1016/j.talo.2025.100560
Aya Barseem , Reem H. Obaydo , Samar H. Elagamy
An environmentally friendly RP-HPLC method was developed for the simultaneous determination of pantoprazole (PAN) and domperidone (DOM). Chromatographic separation was performed using isocratic elution on CORTECS® Shield RP18 column maintained at 25 °C. The mobile phase consisted of ethanol and potassium phosphate buffer (50.0 mM, pH 6.0) in a 30:70 (v/v) ratio, delivered at a flow rate of 1.0 mL/min, with UV detection at 286 nm. The method demonstrated excellent linearity within the concentration ranges of 1.0–25.0 μg/mL for PAN and 3.0–30.0 μg/mL for DOM. It was successfully applied to the analysis of both drugs in bulk and pharmaceutical dosage forms. The greenness of the proposed method was evaluated using the Analytical Eco-Scale, yielding a total score of 83, which indicates minimal environmental impact. The method "whiteness" was assessed using the RGB 12 model, resulting in a high total score of 89.2, reflecting a well-balanced combination of analytical performance, environmental sustainability, and practical implementation. Its "blueness," representing practical applicability, was measured using the BAGI tool and achieved a total score of 82.5. Compared to a previously reported HPLC method, the proposed method offers comparable practical applicability while exhibiting enhanced greenness through the use of ethanol as the organic modifier, rather than environmentally hazardous solvents such as acetonitrile and methanol. The method was fully validated according to ICH Q2(R1) guidelines, confirming its accuracy, precision, specificity, and robustness. Furthermore, the proposed method was statistically compared with a previously reported HPLC method using Student’s t-test and F-test.
{"title":"Eco-friendly HPLC method for simultaneous determination of pantoprazole and domperidone: Comprehensive evaluation of greenness, whiteness, and blueness","authors":"Aya Barseem , Reem H. Obaydo , Samar H. Elagamy","doi":"10.1016/j.talo.2025.100560","DOIUrl":"10.1016/j.talo.2025.100560","url":null,"abstract":"<div><div>An environmentally friendly RP-HPLC method was developed for the simultaneous determination of pantoprazole (PAN) and domperidone (DOM). Chromatographic separation was performed using isocratic elution on CORTECS® Shield RP18 column maintained at 25 °C. The mobile phase consisted of ethanol and potassium phosphate buffer (50.0 mM, pH 6.0) in a 30:70 (v/v) ratio, delivered at a flow rate of 1.0 mL/min, with UV detection at 286 nm. The method demonstrated excellent linearity within the concentration ranges of 1.0–25.0 μg/mL for PAN and 3.0–30.0 μg/mL for DOM. It was successfully applied to the analysis of both drugs in bulk and pharmaceutical dosage forms. The greenness of the proposed method was evaluated using the Analytical Eco-Scale, yielding a total score of 83, which indicates minimal environmental impact. The method \"whiteness\" was assessed using the RGB 12 model, resulting in a high total score of 89.2, reflecting a well-balanced combination of analytical performance, environmental sustainability, and practical implementation. Its \"blueness,\" representing practical applicability, was measured using the BAGI tool and achieved a total score of 82.5. Compared to a previously reported HPLC method, the proposed method offers comparable practical applicability while exhibiting enhanced greenness through the use of ethanol as the organic modifier, rather than environmentally hazardous solvents such as acetonitrile and methanol. The method was fully validated according to ICH Q2(R1) guidelines, confirming its accuracy, precision, specificity, and robustness. Furthermore, the proposed method was statistically compared with a previously reported HPLC method using Student’s <em>t</em>-test and F-test.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100560"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-08-28DOI: 10.1016/j.talo.2025.100538
Xiao-Chen Huang , Ahmed Sobhy Darwish , Wageh Sobhy Darwish , Ri-Meng Chen , Jin-Kui Ma
In addition to providing immediate comfort for dry and irritated eyes, vitamin A (retinol) and E (tocopherol) drops strengthen the eyes' defenses. Optic gels, which are compatible with contact lenses, use vitamins A and E to improve vision, soothe and hydrate dry and sensitive eyes, and provide immediate comfort. It is now feasible to determine the amounts of vitamin A and vitamin E in ocular gels thanks to the development and validation of a thorough, rapid, simple, and eco-friendly stability-indicating LC approach. In compliance with ICH guidelines, the two medications underwent a series of assessments. Vitamin A palmitate, vitamin E acetate, and related degradants were successfully resolved via isocratic separation. Utilizing the octadecylsilyl phase at ambient temperature (25° C ± 5°C), both drugs were quantified and qualified. Constant ratios of filtered water (neutral purified water at pH range 5 to 7) to ethanol comprise the isocratic mobile phase, which has a flow rate of 2.0 mL/min. During the 290 nm detection, the concentrations of vitamin A palmitate and vitamin E acetate varied from 5 to 200 IU/mL and 25 to 1000 μg/mL, respectively. The LC technique's ecological benefits are demonstrated by its overall AES grade of 79, AGREE grade of 0.64, MoGAPI grade of 76, BAGI grade of 80, and final whiteness of 95.8. According to research, the suggested method is accurate, reliable, long-lasting, eco-friendly, and distinctive.
{"title":"Green rapid HPLC method for testing retinol and tocopherol in ophthalmic gels","authors":"Xiao-Chen Huang , Ahmed Sobhy Darwish , Wageh Sobhy Darwish , Ri-Meng Chen , Jin-Kui Ma","doi":"10.1016/j.talo.2025.100538","DOIUrl":"10.1016/j.talo.2025.100538","url":null,"abstract":"<div><div>In addition to providing immediate comfort for dry and irritated eyes, vitamin A (retinol) and E (tocopherol) drops strengthen the eyes' defenses. Optic gels, which are compatible with contact lenses, use vitamins A and E to improve vision, soothe and hydrate dry and sensitive eyes, and provide immediate comfort. It is now feasible to determine the amounts of vitamin A and vitamin E in ocular gels thanks to the development and validation of a thorough, rapid, simple, and eco-friendly stability-indicating LC approach. In compliance with ICH guidelines, the two medications underwent a series of assessments. Vitamin A palmitate, vitamin E acetate, and related degradants were successfully resolved via isocratic separation. Utilizing the octadecylsilyl phase at ambient temperature (25° <em>C</em> ± 5°C), both drugs were quantified and qualified. Constant ratios of filtered water (neutral purified water at pH range 5 to 7) to ethanol comprise the isocratic mobile phase, which has a flow rate of 2.0 mL/min. During the 290 nm detection, the concentrations of vitamin A palmitate and vitamin E acetate varied from 5 to 200 IU/mL and 25 to 1000 μg/mL, respectively. The LC technique's ecological benefits are demonstrated by its overall AES grade of 79, AGREE grade of 0.64, MoGAPI grade of 76, BAGI grade of 80, and final whiteness of 95.8. According to research, the suggested method is accurate, reliable, long-lasting, eco-friendly, and distinctive.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100538"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-05-25DOI: 10.1016/j.talo.2025.100488
Antonia Trisha Zac R, Yao-An Tsai, Chih-Ling Yeh, Hui-Ling Lee
Rational designing of practical and cost-effective electrochemical sensor with simultaneous sensing of ethyl paraben (EPB), diclofenac (DIC), and epinephrine (EPN) remains a challenging aspect. With a motive to solve this concern, a low-cost screen printed electrodes (SPCE) sensor with a novel hybrid Zn-2MI@V-COF-1@MWCNTs modifications was put forward. In this work, an octahedral imidazole based metal organic frameworks (Zn-2MI) is combined with a two-dimensional vinylene based covalent organic frameworks (V-COF-1) to form (Zn-MOF@V-COF-1) through a simple room temperature synthesis. This hybrid is further integrated with multi-walled carbon nanotubes (MWCNTs) for enhanced analyte detection. The structural and crystalline nature of the hybrid materials was analysed using PXRD, SEM, and TEM. The material exhibits ideal porosity and a large electroactive area with multiple adsorption sites, resulting in effective, sensitive detection towards EPN, DIC, and EPB. The sensor achieves low detection limits of 0.05 µM for DIC and EPN and 0.5 µM for EPB. Real samples analysis including chili powder, liquid sweetener and energy drink are conducted using differential pulse voltammetry using satisfactory results ranging recovery rates from 81.54 to 105.8 %. These excellent performance outcomes, attributes to the synergistic effect and the presence of multiple active sites elucidates that novel Zn-2MI@V-COF-1@MWCNTs/SPCE are reliable sensors that offers quick screening, cost effective, sensitive, and allow simultaneous detection of harmful analytes in complex matrices.
{"title":"Heterostructured Zn-2MI@V-COF-1@MWCNTs based electrochemical sensor for the sensitive simultaneous detection of ethyl paraben, diclofenac and epinephrine","authors":"Antonia Trisha Zac R, Yao-An Tsai, Chih-Ling Yeh, Hui-Ling Lee","doi":"10.1016/j.talo.2025.100488","DOIUrl":"10.1016/j.talo.2025.100488","url":null,"abstract":"<div><div>Rational designing of practical and cost-effective electrochemical sensor with simultaneous sensing of ethyl paraben (EPB), diclofenac (DIC), and epinephrine (EPN) remains a challenging aspect. With a motive to solve this concern, a low-cost screen printed electrodes (SPCE) sensor with a novel hybrid Zn-2MI@V-COF-1@MWCNTs modifications was put forward. In this work, an octahedral imidazole based metal organic frameworks (Zn-2MI) is combined with a two-dimensional vinylene based covalent organic frameworks (V-COF-1) to form (Zn-MOF@V-COF-1) through a simple room temperature synthesis. This hybrid is further integrated with multi-walled carbon nanotubes (MWCNTs) for enhanced analyte detection. The structural and crystalline nature of the hybrid materials was analysed using PXRD, SEM, and TEM. The material exhibits ideal porosity and a large electroactive area with multiple adsorption sites, resulting in effective, sensitive detection towards EPN, DIC, and EPB. The sensor achieves low detection limits of 0.05 µM for DIC and EPN and 0.5 µM for EPB. Real samples analysis including chili powder, liquid sweetener and energy drink are conducted using differential pulse voltammetry using satisfactory results ranging recovery rates from 81.54 to 105.8 %. These excellent performance outcomes, attributes to the synergistic effect and the presence of multiple active sites elucidates that novel Zn-2MI@V-COF-1@MWCNTs/SPCE are reliable sensors that offers quick screening, cost effective, sensitive, and allow simultaneous detection of harmful analytes in complex matrices.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100488"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amid rapid industrialization and soaring population growth, there is an ever-increasing global use of medicines that has grown by 14 % over the past five years. The excessive use of pharmaceuticals causes contamination of water bodies through domestic and hospital effluents, as well as by pharmaceutical companies. This in turn threatens global drinking water supplies. Moreover, the biologically active nature of pharmaceutical pollutants (PPs) makes it difficult to degrade in wastewater and hence poses notable risks to both public health and as well as the environment. Given the growing interest in the removal of PPs from wastewater, adsorption is regarded as a simple yet highly effective strategy. In this review, we catalogue recent progress in the adsorptive removal of PPs from wastewater, covering the period from 2013 to the present date. We have systematically covered the comparative overview of existing removal approaches, followed by a detailed examination of various adsorbent materials. Moreover, the influence of porosity, surface area, and functional groups on the adsorption efficiency has been discussed. Likewise, critical considerations, such as optimizing maximum adsorption capacity, the effect of pH, adsorption mechanisms, and adsorbent regeneration, have been analyzed. Moreover, computational and machine-learning–guided molecular design methods in the design of the adsorbent has also been reviewed. Furthermore, the toxicity and hazardous effects of PPs on human health, aquatic ecosystems, and the environment have been extensively discussed. Finally, we outline the disadvantages of the existing adsorbents, elaboration on the research gaps and possible solutions, with the aim to develop more efficient and cost-effective adsorbents in the quest to remove PPs from wastewater.
{"title":"A comprehensive review on the adsorptive removal of pharmaceutical pollutants: Occurrence, toxicology, molecular simulation and mechanistic insights","authors":"Mochamad Lutfi Firmansyah , Yousef Alwan , Nisar Ullah","doi":"10.1016/j.talo.2025.100491","DOIUrl":"10.1016/j.talo.2025.100491","url":null,"abstract":"<div><div>Amid rapid industrialization and soaring population growth, there is an ever-increasing global use of medicines that has grown by 14 % over the past five years. The excessive use of pharmaceuticals causes contamination of water bodies through domestic and hospital effluents, as well as by pharmaceutical companies. This in turn threatens global drinking water supplies. Moreover, the biologically active nature of pharmaceutical pollutants (PPs) makes it difficult to degrade in wastewater and hence poses notable risks to both public health and as well as the environment. Given the growing interest in the removal of PPs from wastewater, adsorption is regarded as a simple yet highly effective strategy. In this review, we catalogue recent progress in the adsorptive removal of PPs from wastewater, covering the period from 2013 to the present date. We have systematically covered the comparative overview of existing removal approaches, followed by a detailed examination of various adsorbent materials. Moreover, the influence of porosity, surface area, and functional groups on the adsorption efficiency has been discussed. Likewise, critical considerations, such as optimizing maximum adsorption capacity, the effect of pH, adsorption mechanisms, and adsorbent regeneration, have been analyzed. Moreover, computational and machine-learning–guided molecular design methods in the design of the adsorbent has also been reviewed. Furthermore, the toxicity and hazardous effects of PPs on human health, aquatic ecosystems, and the environment have been extensively discussed. Finally, we outline the disadvantages of the existing adsorbents, elaboration on the research gaps and possible solutions, with the aim to develop more efficient and cost-effective adsorbents in the quest to remove PPs from wastewater.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100491"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-07-18DOI: 10.1016/j.talo.2025.100519
Ola G. Hussein , Amr M. Mahmoud , Aya A. Mouhamed , Yan Dou , Qiong Nian , Nardine Safwat
Nanozymes engineered to mimic natural enzyme catalysis have emerged as promising alternatives due to their enhanced stability, cost-effectiveness, and tunable catalytic properties compared to biological enzymes. Developing nanozymes with well-defined porous structures and heteroatom doping is key to improving catalytic efficiency and selectivity particularly for applications in biosensing. Nanotechnology has become a key enabler in advancing catalysis, biosensing, and environmental monitoring thanks to the exceptional properties of nanomaterials such as large surface area, customizable optical characteristics, and enhanced catalytic activity. In this study, a novel nanozyme platform based on creatinine–thiourea–FeCl3 nanoparticles (CTF-NPs) was developed via a one-step pyrolysis method using colloidal silica as a porosity template. The synthesized CTF-NPs were uniformly structured as confirmed by SEM, EDX, FT-IR, UV–Vis, and XRD and featured effective nitrogen, sulfur, and iron doping contributing to their catalytic efficiency. The CTF-NPs exhibited strong peroxidase-like activity catalyzing the colorimetric oxidation of o-phenylenediamine (OPD) in the presence of hydrogen peroxide (H2O2). Kinetic studies revealed a Km of 0.819 mM and a Vmax of 1.620 μM/min outperforming several previously reported nanozyme systems. The sensor displayed a linear detection range of 10–700 μM for H2O2 with an excellent correlation coefficient (r= 0.9975) optimal at pH 4, 40 °C, and 10-minute incubation. Upon coupling with glucose oxidase (GOx), the platform enabled indirect glucose detection via enzymatically generated H2O2 showing a linear range of 100–600 μM with a correlation coefficient of r= 0.9953. The system exhibited high selectivity against common interferents, reproducible fabrication, and reliable performance in spiked human serum samples. Integration of the nanozyme system with a portable RGB color sensor (TCS34725) enabled quantitative, user-friendly, and instrument-free detection supporting the development of accessible point-of-care diagnostics. This work introduces a scalable and robust platform for glucose monitoring with promising implications for diabetes management and decentralized healthcare.
{"title":"Arduino-based portable point-of-care colorimetric glucose biosensor using nanozyme with enhanced peroxidase-like activity","authors":"Ola G. Hussein , Amr M. Mahmoud , Aya A. Mouhamed , Yan Dou , Qiong Nian , Nardine Safwat","doi":"10.1016/j.talo.2025.100519","DOIUrl":"10.1016/j.talo.2025.100519","url":null,"abstract":"<div><div>Nanozymes engineered to mimic natural enzyme catalysis have emerged as promising alternatives due to their enhanced stability, cost-effectiveness, and tunable catalytic properties compared to biological enzymes. Developing nanozymes with well-defined porous structures and heteroatom doping is key to improving catalytic efficiency and selectivity particularly for applications in biosensing. Nanotechnology has become a key enabler in advancing catalysis, biosensing, and environmental monitoring thanks to the exceptional properties of nanomaterials such as large surface area, customizable optical characteristics, and enhanced catalytic activity. In this study, a novel nanozyme platform based on creatinine–thiourea–FeCl<sub>3</sub> nanoparticles (CTF-NPs) was developed via a one-step pyrolysis method using colloidal silica as a porosity template. The synthesized CTF-NPs were uniformly structured as confirmed by SEM, EDX, FT-IR, UV–Vis, and XRD and featured effective nitrogen, sulfur, and iron doping contributing to their catalytic efficiency. The CTF-NPs exhibited strong peroxidase-like activity catalyzing the colorimetric oxidation of <em>o</em>-phenylenediamine (OPD) in the presence of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Kinetic studies revealed a K<sub>m</sub> of 0.819 mM and a V<sub>max</sub> of 1.620 μM/min outperforming several previously reported nanozyme systems. The sensor displayed a linear detection range of 10–700 μM for H<sub>2</sub>O<sub>2</sub> with an excellent correlation coefficient (r= 0.9975) optimal at pH 4<strong>,</strong> 40 °C<strong>,</strong> and 10-minute incubation. Upon coupling with glucose oxidase (GOx), the platform enabled indirect glucose detection via enzymatically generated H<sub>2</sub>O<sub>2</sub> showing a linear range of 100–600 μM with a correlation coefficient of r= 0.9953. The system exhibited high selectivity against common interferents, reproducible fabrication, and reliable performance in spiked human serum samples. Integration of the nanozyme system with a portable RGB color sensor (TCS34725) enabled quantitative, user-friendly, and instrument-free detection supporting the development of accessible point-of-care diagnostics. This work introduces a scalable and robust platform for glucose monitoring with promising implications for diabetes management and decentralized healthcare.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100519"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-06-09DOI: 10.1016/j.talo.2025.100499
Ahmed S. El-tahlawy , Abdullah S. Alawam , Hassan A. Rudayn , Ahmed A. Allam , Rehab Mahmoud , Hany Abd El-Raheem , Waleed Alahmad
The integrity of the global food supply is increasingly compromised by sophisticated fraudulent methods such as adulteration, mislabeling, and substitution. These deceptive practices introduce undisclosed and harmful substances that often evade traditional detection, posing significant risks to public health and consumer trust. This review synthesizes cutting-edge advancements in analytical and digital technologies that are redefining food fraud surveillance. We explore the convergence of molecular diagnostics (e.g., DNA barcoding, clustered regularly interspaced short palindromic repeats (CRISPR), next-generation sequencing (NGS)), high-throughput spectroscopic platforms (e.g., Raman spectroscopy, mass spectrometry), biosensors, and digital innovations (e.g., artificial intelligence (AI), Internet of Things (IoT), blockchain, and lab-on-a-chip (LOC) devices). These integrated approaches enable real-time, proactive detection of fraud across complex and globalized supply chains, offering sensitivity, scalability, and traceability previously unattainable. By framing food fraud as a form of emerging contamination, this work aligns with the paradigm shift toward predictive, data-driven risk assessment in contaminant detection. The review also addresses key translational bottlenecks such as cost, standardization, and interoperability, and proposes a systems-level roadmap to bridge scientific innovation with regulatory and industrial application. This interdisciplinary perspective advocates for a future where food authenticity assurance is intelligent, holistic, and resilient in the face of evolving adulteration threats.
{"title":"Advanced analytical and digital approaches for proactive detection of food fraud as an emerging contaminant threat","authors":"Ahmed S. El-tahlawy , Abdullah S. Alawam , Hassan A. Rudayn , Ahmed A. Allam , Rehab Mahmoud , Hany Abd El-Raheem , Waleed Alahmad","doi":"10.1016/j.talo.2025.100499","DOIUrl":"10.1016/j.talo.2025.100499","url":null,"abstract":"<div><div>The integrity of the global food supply is increasingly compromised by sophisticated fraudulent methods such as adulteration, mislabeling, and substitution. These deceptive practices introduce undisclosed and harmful substances that often evade traditional detection, posing significant risks to public health and consumer trust. This review synthesizes cutting-edge advancements in analytical and digital technologies that are redefining food fraud surveillance. We explore the convergence of molecular diagnostics (e.g., DNA barcoding, clustered regularly interspaced short palindromic repeats (CRISPR), next-generation sequencing (NGS)), high-throughput spectroscopic platforms (e.g., Raman spectroscopy, mass spectrometry), biosensors, and digital innovations (e.g., artificial intelligence (AI), Internet of Things (IoT), blockchain, and lab-on-a-chip (LOC) devices). These integrated approaches enable real-time, proactive detection of fraud across complex and globalized supply chains, offering sensitivity, scalability, and traceability previously unattainable. By framing food fraud as a form of emerging contamination, this work aligns with the paradigm shift toward predictive, data-driven risk assessment in contaminant detection. The review also addresses key translational bottlenecks such as cost, standardization, and interoperability, and proposes a systems-level roadmap to bridge scientific innovation with regulatory and industrial application. This interdisciplinary perspective advocates for a future where food authenticity assurance is intelligent, holistic, and resilient in the face of evolving adulteration threats.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100499"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-07-08DOI: 10.1016/j.talo.2025.100511
Hadeer M. Bedair , Mohamed Emara , Tamer M. Samir , Mahmoud A.F. Khalil , Shima Mahmoud Ali , Mahmoud Hamed , Fotouh R. Mansour
Food safety continues to be a critical global challenge, with foodborne pathogens posing significant risks to public health and economic stability. The rapid detection of these pathogens, especially in resource-limited settings, is essential to mitigate outbreaks and enhance food safety systems. This review explores the advancements in paper-based analytical devices (PADs) as a practical, cost-effective, and portable solution for the detection of foodborne pathogens. Various detection platforms, including nucleic acid-based sensors, aptamer-integrated systems, Immunochromatographic assays, and nanoparticle-enhanced biosensors, are evaluated for their effectiveness in rapid and on-site testing. PADs meet the key requirements for point-of-need testing, including simplicity, speed, affordability, and versatility. Their ability to integrate with modern technologies such as smartphones and machine learning enhances their analytical performance and accessibility. Despite their promise, challenges remain, including sensitivity limitations and scalability. Addressing these gaps could unlock the full potential of PADs for widespread use in food safety monitoring and pathogen detection.
{"title":"Paper-based analytical devices for smart foodborne pathogen detection","authors":"Hadeer M. Bedair , Mohamed Emara , Tamer M. Samir , Mahmoud A.F. Khalil , Shima Mahmoud Ali , Mahmoud Hamed , Fotouh R. Mansour","doi":"10.1016/j.talo.2025.100511","DOIUrl":"10.1016/j.talo.2025.100511","url":null,"abstract":"<div><div>Food safety continues to be a critical global challenge, with foodborne pathogens posing significant risks to public health and economic stability. The rapid detection of these pathogens, especially in resource-limited settings, is essential to mitigate outbreaks and enhance food safety systems. This review explores the advancements in paper-based analytical devices (PADs) as a practical, cost-effective, and portable solution for the detection of foodborne pathogens. Various detection platforms, including nucleic acid-based sensors, aptamer-integrated systems, Immunochromatographic assays, and nanoparticle-enhanced biosensors, are evaluated for their effectiveness in rapid and on-site testing. PADs meet the key requirements for point-of-need testing, including simplicity, speed, affordability, and versatility. Their ability to integrate with modern technologies such as smartphones and machine learning enhances their analytical performance and accessibility. Despite their promise, challenges remain, including sensitivity limitations and scalability. Addressing these gaps could unlock the full potential of PADs for widespread use in food safety monitoring and pathogen detection.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100511"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Unlocking the power of nature, this study introduces pyoverdine, a naturally derived bacterial siderophore, as a green solvent for the FRAP assay, marking a significant step toward sustainable and ecofriendly antioxidant testing. This study developed and validated a novel Pyoverdine-Ferric Reducing Antioxidant Power assay for assessing antioxidant activity. Pyoverdine, a secondary metabolite secreted by Pseudomonas taiwanensis R-12–2, was successfully isolated and screened for antioxidant potential. In this study we have evaluated the antioxidant activity of three standard antioxidants like gallic acid, tannic acid, and quercetin using both the traditional Ferric Reducing Antioxidant Power (FRAP) assay and a novel pyoverdine-based Ferric Reducing Antioxidant Power (PFrAP) assay. The FRAP assay demonstrated significant reducing power for all three compounds, with gallic acid showing the highest antioxidant activity, reflected by an EC50 value of 42.28±0.15 μM. Tannic acid and quercetin exhibited EC50 values of 48.03±0.78 μM and 49.81±0.25 μM, respectively. The PFrAP assay showed similar results, with EC50 values of 42.54±0.71 μM for gallic acid, 49.96±3.88 μM for tannic acid, and 50.27±1.40 μM for quercetin. This innovative approach not only reduces environmental impact but also holds broad potential for application in pharmaceutical, nutraceutical, and food industries, paving the way for a new era of green analytical techniques for antioxidant evaluation.
{"title":"Bacterial siderophore as a green solvent for the development of FRAP assay as a potential antioxidant test: A comparative study","authors":"Nisha Nerlekar , Prafull Dandge , Vinod Nandre , Padma Dandge","doi":"10.1016/j.talo.2025.100515","DOIUrl":"10.1016/j.talo.2025.100515","url":null,"abstract":"<div><div>Unlocking the power of nature, this study introduces pyoverdine, a naturally derived bacterial siderophore, as a green solvent for the FRAP assay, marking a significant step toward sustainable and ecofriendly antioxidant testing. This study developed and validated a novel Pyoverdine-Ferric Reducing Antioxidant Power assay for assessing antioxidant activity. Pyoverdine, a secondary metabolite secreted by <em>Pseudomonas taiwanensis</em> R-12–2, was successfully isolated and screened for antioxidant potential. In this study we have evaluated the antioxidant activity of three standard antioxidants like gallic acid, tannic acid, and quercetin using both the traditional Ferric Reducing Antioxidant Power (FRAP) assay and a novel pyoverdine-based Ferric Reducing Antioxidant Power (PFrAP) assay. The FRAP assay demonstrated significant reducing power for all three compounds, with gallic acid showing the highest antioxidant activity, reflected by an EC<sub>50</sub> value of 42.28±0.15 μM. Tannic acid and quercetin exhibited EC<sub>50</sub> values of 48.03±0.78 μM and 49.81±0.25 μM, respectively. The PFrAP assay showed similar results, with EC<sub>50</sub> values of 42.54±0.71 μM for gallic acid, 49.96±3.88 μM for tannic acid, and 50.27±1.40 μM for quercetin. This innovative approach not only reduces environmental impact but also holds broad potential for application in pharmaceutical, nutraceutical, and food industries, paving the way for a new era of green analytical techniques for antioxidant evaluation.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100515"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-06-25DOI: 10.1016/j.talo.2025.100507
Arzum Erdem, Huseyin Senturk, Mehmet Karakus
Molecularly imprinted polymers (MIPs) have emerged in recent years as highly promising materials for sensor design, owing to their high selectivity, stability, and reusability toward target analytes. This review specifically focuses on MIP-based sensor applications aimed at detecting critically important biomolecules such as proteins and DNA, which play essential roles especially in the biomedical field. Although antibody-based immunoassays for protein detection and PCR-based methods for DNA analysis provide high specificity and sensitivity, these conventional approaches have significant limitations, including high costs, limited stability, complex instrumentation, and the necessity of highly skilled personnel. MIPs have recently gained attention as synthetic recognition elements capable of overcoming these limitations. The rationale behind jointly addressing protein and DNA analysis lies primarily in the shared challenges presented by these biomolecules, such as molecular size, structural complexity, and specificity of binding. Furthermore, similar analytical approaches and transduction mechanisms employed in the sensor designs for these two groups allow for a more comprehensive and integrated evaluation. This review thoroughly examines MIP structures integrated into electrochemical, optical, quartz crystal microbalance (QCM), and other sensor platforms. Current limitations such as heterogeneity of binding sites and incomplete removal of template molecules are critically discussed, alongside proposed solutions like incorporation of nanomaterials, computational modeling, and novel polymerization strategies. In conclusion, this review provides an extensive evaluation of recent advances in protein and DNA detection using MIP-based sensors, clearly outlining the current state, encountered challenges, and future perspectives within the field.
{"title":"Molecularly imprinted polymer-based sensors: Design and advances in the analysis of DNA and protein","authors":"Arzum Erdem, Huseyin Senturk, Mehmet Karakus","doi":"10.1016/j.talo.2025.100507","DOIUrl":"10.1016/j.talo.2025.100507","url":null,"abstract":"<div><div>Molecularly imprinted polymers (MIPs) have emerged in recent years as highly promising materials for sensor design, owing to their high selectivity, stability, and reusability toward target analytes. This review specifically focuses on MIP-based sensor applications aimed at detecting critically important biomolecules such as proteins and DNA, which play essential roles especially in the biomedical field. Although antibody-based immunoassays for protein detection and PCR-based methods for DNA analysis provide high specificity and sensitivity, these conventional approaches have significant limitations, including high costs, limited stability, complex instrumentation, and the necessity of highly skilled personnel. MIPs have recently gained attention as synthetic recognition elements capable of overcoming these limitations. The rationale behind jointly addressing protein and DNA analysis lies primarily in the shared challenges presented by these biomolecules, such as molecular size, structural complexity, and specificity of binding. Furthermore, similar analytical approaches and transduction mechanisms employed in the sensor designs for these two groups allow for a more comprehensive and integrated evaluation. This review thoroughly examines MIP structures integrated into electrochemical, optical, quartz crystal microbalance (QCM), and other sensor platforms. Current limitations such as heterogeneity of binding sites and incomplete removal of template molecules are critically discussed, alongside proposed solutions like incorporation of nanomaterials, computational modeling, and novel polymerization strategies. In conclusion, this review provides an extensive evaluation of recent advances in protein and DNA detection using MIP-based sensors, clearly outlining the current state, encountered challenges, and future perspectives within the field.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100507"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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