Talanta Open最新文献_第5页

Analytical methodologies for antioxidant capacity assessment: An updated review 抗氧化能力评估的分析方法：最新综述

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-14 DOI: 10.1016/j.talo.2025.100558

Mahmoud Hamed , Faisal K. Algethami , Alaa Bedair , Fotouh R. Mansour

Antioxidants are crucial molecules protecting biological systems from harmful oxidation reactions and free radicals. This class of compounds attracted remarkable attention for their potential in functional foods and dietary supplements. The imbalance between oxidants and antioxidants leads to oxidative stress, associated with various chronic diseases. Accurate measurement of antioxidant activity is therefore essential for evaluating potential health-enhancing agents in food science, medicine, and biotechnology. This review provides a comprehensive overview of contemporary analytical methodologies for assessing antioxidant capacity across food, cosmetic, pharmaceutical, and biomedical applications, exploring four major categories of techniques: spectrophotometric methods, including UV–Vis, electron paramagnetic resonance (EPR), and near infrared (NIR) spectroscopy, highlighting their principles and applications in radical scavenging assays and antioxidant characterization. Electrochemical techniques are rapid, sensitive, and cost-effective alternatives, focusing on voltammetry, amperometry, and biosensor development for direct measurement of electron transfer processes. The role of biosensors, employing bioreceptors like enzymes and DNA, for selective and sensitive antioxidant detection is also examined. Chromatographic methods, such as high-performance liquid chromatography (HPLC) and gas chromatography (GC), paired with selective detectors, are used to separate, identify, and quantify antioxidant compounds in complex matrices. This review highlights the principles, advancements, advantages, and limitations of these diverse methodologies in the accurate evaluation of antioxidant potential.

抗氧化剂是保护生物系统免受有害氧化反应和自由基侵害的关键分子。这类化合物因其在功能性食品和膳食补充剂中的潜力而备受关注。氧化剂和抗氧化剂之间的不平衡导致氧化应激，与各种慢性疾病有关。因此，准确测量抗氧化活性对于评估食品科学、医学和生物技术中潜在的健康促进剂至关重要。本文综述了食品、化妆品、制药和生物医学领域抗氧化能力评估的现代分析方法，探讨了四大类技术：分光光度法，包括紫外-可见、电子顺磁共振（EPR）和近红外（NIR）光谱，重点介绍了它们在自由基清除试验和抗氧化表征中的原理和应用。电化学技术是一种快速、灵敏、经济的替代方法，主要集中在伏安法、安培法和生物传感器的开发上，用于直接测量电子转移过程。生物传感器的作用，采用生物受体如酶和DNA，选择性和敏感的抗氧化剂检测也进行了检查。色谱方法，如高效液相色谱（HPLC）和气相色谱（GC），与选择性检测器配对，用于分离、鉴定和定量复杂基质中的抗氧化化合物。本文综述了这些不同方法在准确评价抗氧化潜力方面的原理、进展、优势和局限性。

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引用次数: 0

Unveiling the role of α- and β-CDs in gold nanoparticle gel-based sensors for Fe³⁺ colorimetric detection 揭示α-和β-CDs在用于Fe +比色检测的金纳米颗粒凝胶传感器中的作用

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-13 DOI: 10.1016/j.talo.2025.100557

Agustina Sus Andreani , Farrah Nurkhaliza , Muhammad Ridwan , Salsabila Freya Daniarti , Lilis Rosmaniar , Meiyanti Ratna Kumalasari

The detection of Fe³⁺ in water samples is critical for mitigating potential harm to environmental and human health. This study presents a comparative investigation of α-cyclodextrin (α-CDs) and β-cyclodextrin (β-CDs) as stabilizing agents in the development of gold nanoparticle (AuNPs)-based gel sensors for Fe³⁺ colorimetric sensing. The AuNPs were synthesized using ortho-hydroxybenzoic acid (o-HBA) as a reducing agent. The red-wine colloidal solutions of AuNPs-o-αCDs and AuNPs-o-βCDs were measured by a UV–Vis spectrophotometer, showing a surface plasmon resonance (SPR) band at 517 nm for both. Field emission scanning electron microscopy – energy dispersive X-ray (FESEM-EDX) analysis revealed well-dispersed AuNPs within the gel matrix. Particle size analysis (PSA) and zeta potential measurements further indicated a smaller particle size and superior colloidal stability in the AuNPs-o-βCDs. The analytical performance of both sensors was evaluated through precision, accuracy (%Recovery), and comparison with atomic absorption spectroscopy (AAS) via t-test assessments. Both sensors demonstrated acceptable precision and accuracy, showing no statistically significant difference from AAS results (p > 0.05). Notably, the gel-AuNPs-o-βCDs sensor exhibited enhanced sensitivity (0.20 mg/L) and faster detection, achieving stable readings within 15 min. In contrast, the gel-AuNPs-o-αCDs had a LoD of 0.57 mg/L and required 20 min for stable readout. Additionally, the gel-AuNPs-o-βCDs demonstrated a higher linear correlation with Fe³⁺ concentration (R² = 0.9999) than the gel-AuNPs-o-αCDs (R² = 0.9994). These findings suggested that β-CDs provide superior stabilization and enhanced performance in the gel-based AuNPs sensors, establishing them as a highly promising candidate for rapid, reliable, and accurate Fe³⁺ detection in tap water.

水样中Fe3+的检测对于减轻对环境和人类健康的潜在危害至关重要。本研究比较了α-环糊精（α-CDs）和β-环糊精（β-CDs）作为稳定剂在制备纳米金（AuNPs）凝胶传感器中对Fe3+比色的影响。以邻羟基苯甲酸（o-HBA）为还原剂合成了AuNPs。用紫外-可见分光光度计测定了AuNPs-o-αCDs和AuNPs-o-βCDs的红酒胶体溶液，两者在517 nm处均有表面等离子体共振（SPR）带。场发射扫描电子显微镜-能量色散x射线（FESEM-EDX）分析显示凝胶基质中分布良好的aunp。粒径分析（PSA）和zeta电位测量进一步表明，AuNPs-o-βCDs具有更小的粒径和更好的胶体稳定性。两种传感器的分析性能通过精密度、准确度（回收率%）和通过t检验评估与原子吸收光谱（AAS）的比较来评估。两种传感器的精度和准确度均可接受，与原子吸收光谱结果无统计学差异（p > 0.05）。值得注意的是，凝胶- aunps -o-βCDs传感器具有更高的灵敏度（0.20 mg/L）和更快的检测速度，可在15分钟内获得稳定读数。相比之下，凝胶- aunps -o-αCDs的LoD为0.57 mg/L，需要20 min才能稳定读数。此外，凝胶- aunps -o-βCDs与Fe3+浓度的线性相关（R2 = 0.9999）高于凝胶- aunps -o-αCDs （R2 = 0.9994）。这些发现表明，β-CDs在凝胶型AuNPs传感器中提供了优越的稳定性和增强的性能，使其成为快速、可靠和准确检测自来水中Fe3+的极有希望的候选材料。

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引用次数: 0

Stability and reproducibility study for the development of a potentiometric nitrate sensor for in-situ use 原位使用电位式硝酸盐传感器的稳定性和重复性研究

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-11 DOI: 10.1016/j.talo.2025.100556

Michelle Brandão Silva de Assis , Kathrin Trommer , Alfred Kick , Michael Mertig

This study investigates the stability and reproducibility of a potentiometric nitrate sensor in all-solid-state configuration, consisting of a screen-printed graphite electrode, coated with electropolymerized polypyrrole as solid contact material, and covered by a TDMA-based ion-selective membrane. Special attention is given to the long-term stability, depending on storage and conditioning conditions as the most important factors for the applicability of the sensor. In particular, regression lines from calibration procedures, performed over a period of up to three months, were analyzed to evaluate the sensor performance. The sensor demonstrated superior stability, with minimal, nearly parallel shifts between regression lines. Notably, the sensor retained its ability to reproduce signals accurately even after one-month periods of dry storage, provided that the applied conditioning period was sufficiently long. The sensor was successfully applied for nitrate detection in drinking water samples, with a reproducibility of ± 3 mg/L, making it a promising candidate for real-time nitrate sensing applications. In all stages of the experiments, the sensor performance was compared with that of an all-solid-state sensor system, consisting of a gold electrode coated with poly(3-octylthiophene-2,5-diyl) and molybdenum disulfide nanocomposites as solid contact material.

本研究研究了一种全固态电位硝酸盐传感器的稳定性和再现性，该传感器由丝网印刷石墨电极组成，表面涂有电聚合聚吡咯作为固体接触材料，表面覆盖一层基于tdma的离子选择膜。特别注意的是长期稳定性，这取决于存储和调节条件作为传感器适用性的最重要因素。特别是，对校准过程中的回归线进行了长达三个月的分析，以评估传感器的性能。该传感器表现出优越的稳定性，在回归线之间有最小的几乎平行的位移。值得注意的是，即使在一个月的干燥储存期后，只要施加的调理期足够长，传感器仍能准确地再现信号。该传感器成功应用于饮用水样品的硝酸盐检测，重现性为±3 mg/L，是实时硝酸盐传感应用的理想选择。在实验的各个阶段，传感器的性能与全固态传感器系统的性能进行了比较，全固态传感器系统由涂有聚（3-辛基噻吩-2,5-二酰基）和二硫化钼纳米复合材料作为固体接触材料的金电极组成。

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引用次数: 0

SensEDNA: An innovative optical CRISPR platform for rapid environmental DNA monitoring SensEDNA：一个创新的光学CRISPR平台，用于快速环境DNA监测

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-10 DOI: 10.1016/j.talo.2025.100551

Molly Ann Williams , Ciprian Briciu-Burghina , Sean Power , Joyce O’Grady , Elvira de Eyto , Nigel Kent , Anne Parle-McDermott , Fiona Regan

Biodiversity loss is at an all-time high increasing the need for simple and rapid species monitoring systems. Management and conservation of fish species within aquatic environments requires knowledge of distribution, traditionally gained through visual detection and counting. These methods are expensive, time consuming and can lead to harm of the species of interest. Environmental DNA (eDNA) offers a solution to this through detection of DNA that may be shed into the environment by a given target species. Integrating eDNA based molecular assays to a biosensor device enables onsite sample testing for rapid species assessment.

Herein, a portable fluorometer with incubating capabilities (SensEDNA) was custom designed and built to conduct the simultaneous incubation and fluorescent detection steps required for isothermal eDNA detection. The system features three optical cells to enable triplicate analysis. Each optical cell is set to incubate at 37 °C and control within 0.5 °C. Fluorescence detection with excitation at 485 nm and emission at 535 nm was integrated and optimised to provide highest signal/noise ratio while minimising fluorescence bleaching. The system comprises a simple LED/high pass filter/photodiode configuration and is controlled by a Wixel board. This affordable and user-friendly optical detection platform, coupled with RPA-CRISPR-Cas, provides a crucial step towards on-site single species identification from eDNA.

生物多样性的丧失正处于空前的高度，增加了对简单和快速物种监测系统的需求。水生环境中鱼类的管理和保护需要有关分布的知识，传统上是通过目测和计数获得的。这些方法成本高，耗时长，而且可能导致对感兴趣的物种的伤害。环境DNA （eDNA）通过检测可能由给定目标物种释放到环境中的DNA提供了解决方案。将基于eDNA的分子分析集成到生物传感器设备中，可以进行现场样品测试，以进行快速物种评估。为此，我们定制了一种具有孵育功能的便携式荧光仪（SensEDNA），用于同时进行等温eDNA检测所需的孵育和荧光检测步骤。该系统具有三个光学单元，可以进行三次重复分析。每个光学细胞设置在37°C孵育，控制在0.5°C内。集成并优化了485 nm激发和535 nm发射的荧光检测，以提供最高的信噪比，同时最大限度地减少荧光漂白。该系统包括一个简单的LED/高通滤波器/光电二极管配置，并由一个Wixel板控制。这种价格合理且用户友好的光学检测平台，加上RPA-CRISPR-Cas，为eDNA的现场单物种鉴定提供了关键的一步。

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引用次数: 0

Transforming a traditional ethnic beverage into highly emissive CDs as a sensitive nanosensor for the anti-malignant hyperthermia drug dantrolene sodium in pharmaceuticals and plasma. An economic and sustainable approach 将传统民族饮料转化为高发射cd，作为药物和血浆中抗恶性热疗药物丹曲林钠的敏感纳米传感器。经济和可持续的方法

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-09 DOI: 10.1016/j.talo.2025.100549

Samar Salem, Rania El-Shaheny, Nora A. Abdallah

This work provides a cost-effective, environmentally benign, rapidly prepared, highly fluorescent carbon dots (CDs) using renewable antioxidants-rich feedstock (Tamarindus Indica L.) as the precursor. A home-available and less energy-consuming microwave oven synthesizes CDs within 5 min. The created CDs have the highest fluorescence intensity at 402 nm after being excited at 328 nm, conferring a high luminescence quantum yield of 33.5 %. The prepared CDs provide ultra-sensitive and selective determination of dantrolene sodium (DAN), the only synthetic drug that can manage malignant hyperthermia up to now, and of extraordinary antioxidant properties. The determination is over a wide concentration range of (1.25–100.0 µM) with a limit of detection (LOD) and limit of quantification (LOQ) of 0.07 µM and 0.21 µM, respectively. DAN was estimated in two different pharmaceutical dosage forms, yielding percentage recoveries of 99.49 ± 1.50 and 99.74 ± 0.97. The proposed method was also successfully applied for the assay of DAN in spiked human plasma with a % recovery of 99.55 ± 3.13. Various greenness metrics were used to elucidate the greenness of the nanoprobe. The greenness of this work stems from the fabrication of CDs from natural, widespread, cheap, and edible fruit using a less energy and time-consuming, easily operated, and safe reactor. Moreover, avoiding harsh chemicals and solvents and using distilled water as the diluent renders the method greener. The above-mentioned facts about the proposed method prove it to be a validated analytical approach for different pharmaceutical and biological applications.

本研究提供了一种经济、环保、快速制备的高荧光碳点（CDs），该碳点以可再生的富含抗氧化剂的原料为前体。家用的低能耗微波炉可以在5分钟内合成cd。所制备的CDs在328 nm处激发后，在402 nm处荧光强度最高，发光量子产率高达33.5%。所制备的CDs具有优异的抗氧化性能，是迄今为止唯一用于治疗恶性高热的合成药物丹曲林钠（dantrolene sodium， DAN）的超灵敏和选择性测定。测定范围宽（1.25 ~ 100.0µM），检测限（LOD）为0.07µM，定量限（LOQ）为0.21µM。测定了两种不同剂型的DAN，回收率分别为99.49±1.50和99.74±0.97。该方法可用于加标血浆中DAN的测定，回收率为99.55±3.13 %。采用不同的绿色度指标来说明纳米探针的绿色度。这项工作的绿色源于用天然、广泛、廉价和可食用的水果制作cd，使用更少的能源和时间，易于操作和安全的反应器。此外，避免了苛刻的化学品和溶剂，并使用蒸馏水作为稀释剂，使该方法更加环保。上述事实证明，该方法是一种有效的分析方法，适用于不同的制药和生物学应用。

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引用次数: 0

Plasmonic fingerprinting: next-generation SERS architectures for sensitive heavy metal quantification 等离子体指纹识别：用于敏感重金属量化的下一代SERS架构

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-09 DOI: 10.1016/j.talo.2025.100554

Muhammad Sarkawi , Rupam Sharma , Geetha Venkatesan , Tripti Ahuja , Soumyanti Panda , Jai Prakash , Hideki Kuramitz , K.S. Shalini devi

The global heavy metal crisis demands sensing technologies that transcend traditional analytical limitations, positioning Surface-Enhanced Raman Scattering (SERS) as the transformative solution for real-world environmental and health monitoring challenges. This comprehensive review illuminates the sophisticated engineering principles underlying modern SERS architectures, where nanoscale precision meets molecular recognition to achieve detection limits that challenge the boundaries of single-molecule spectroscopy. Additionally, a systematic analysis of various SERS sensing strategies, including label-free approaches utilizing direct metal-nanoparticle interactions, molecular probe-based systems employing Raman-active chelating agents, and advanced functionalized nanoparticle platforms incorporating DNA aptamers, peptides, and polymeric recognition elements are discussed. Representative case studies include the successful detection of mercury in contaminated fish samples at sub-regulatory levels, lead quantification in urban drinking water systems, and arsenic monitoring in rice cultivation areas, showcasing the versatility and reliability of SERS-based approaches across diverse analytical challenges. The review concludes with a progressive perspective on the merging of SERS technology with microfluidics, Internet of Things architectures, and distributed sensing networks that will replace the current approach of testing samples periodically with continuous monitoring of heavy metals at specific locations.

全球重金属危机需要超越传统分析限制的传感技术，将表面增强拉曼散射（SERS）定位为现实世界环境和健康监测挑战的变革性解决方案。这篇全面的综述阐明了现代SERS架构背后的复杂工程原理，其中纳米级精度满足分子识别，以实现挑战单分子光谱边界的检测极限。此外，本文还讨论了各种SERS传感策略的系统分析，包括利用直接金属-纳米颗粒相互作用的无标记方法，利用拉曼活性螯合剂的基于分子探针的系统，以及结合DNA适体、肽和聚合物识别元件的高级功能化纳米颗粒平台。代表性案例研究包括成功检测亚监管水平受污染鱼类样本中的汞，城市饮用水系统中的铅量化以及水稻种植区域的砷监测，展示了基于sers的方法在各种分析挑战中的多功能性和可靠性。该综述总结了SERS技术与微流体、物联网架构和分布式传感网络相结合的进步观点，这些技术将取代目前定期检测样品并在特定位置连续监测重金属的方法。

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引用次数: 0

Fc-tagged PLA2R antigen enables high-performance chemiluminescent detection for non-invasive idiopathic membranous nephropathy diagnosis fc标记的PLA2R抗原可用于非侵袭性特发性膜性肾病的高效化学发光检测

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-09 DOI: 10.1016/j.talo.2025.100555

Jiangjian Liu , Qiong Liang , Yanhang Zhuo, Sunhui Chen

Idiopathic membranous nephropathy (IMN) is characterized by anti-phospholipase A2 receptor (PLA2R) antibodies, yet current detection methods lack sensitivity or are invasive. We developed a chemiluminescent assay to address these limitations. Three PLA2R antigens (Fc-tagged full, His-tagged, D3 core segment) were expressed in Expi293E cells and purified. Goat/mouse anti-human IgG antibodies were compared for detection efficiency. Kit stability was evaluated at 4 °C and 37 °C. Clinical validation included 75 biopsy-confirmed cases (50 IMN, 25 non-IMN), comparing the kit’s performance against a commercial ELISA. The PLA2R-Fc antigen demonstrated 100 % sensitivity and specificity, outperforming His-tagged (100 % sensitivity, 97 % specificity) and D3-truncated variants (60 % sensitivity). Coating in pH 6.0 MES buffer achieved the highest signal-to-noise ratio (45.08 vs. 25.76 in HEPES). Goat anti-human IgG enabled lower detection limits (22.5 U mL⁻¹ vs. 45 U mL⁻¹ for monoclonal antibody). The kit retained stable over 12 months at 4 °C. In clinical testing, the chemiluminescent assay showed superior concordance with histopathology, higher sensitivity, and specificity, particularly for low-titer samples. This chemiluminescent anti-PLA2R antibody assay combines high sensitivity, specificity, and stability, enabling non-invasive IMN diagnosis and therapeutic monitoring. Its technical optimizations address key limitations of existing methods, offering a reliable diagnostic tool for IMN.

特发性膜性肾病（IMN）以抗磷脂酶A2受体（PLA2R）抗体为特征，但目前的检测方法缺乏敏感性或具有侵入性。我们开发了一种化学发光试验来解决这些局限性。三种PLA2R抗原（fc标记full， his标记，D3核心段）在Expi293E细胞中表达并纯化。比较山羊/小鼠抗人IgG抗体的检测效率。在4°C和37°C时评估试剂盒的稳定性。临床验证包括75例活检确诊病例（50例IMN， 25例非IMN），将试剂盒的性能与商业ELISA进行比较。PLA2R-Fc抗原表现出100%的敏感性和特异性，优于his标记的（100%敏感性，97%特异性）和d3截断的变体（60%敏感性）。在pH 6.0的MES缓冲液中涂层获得了最高的信噪比（45.08 vs. 25.76 HEPES）。山羊抗人IgG的检出限较低（单克隆抗体为22.5 U mL−1，单克隆抗体为45 U mL−1）。试剂盒在4°C下保持稳定超过12个月。在临床测试中，化学发光法显示出与组织病理学的优越一致性，更高的灵敏度和特异性，特别是对低滴度样品。这种化学发光抗pla2r抗体检测结合了高灵敏度、特异性和稳定性，使非侵入性IMN诊断和治疗监测成为可能。其技术优化解决了现有方法的主要局限性，为IMN提供了可靠的诊断工具。

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引用次数: 0

Validation of combination of protodioscin and shatavarin iv from medicinal extracts for alleviating menopausal symptoms by computational deep learning models 通过计算深度学习模型验证药用提取物中原薯蓣皂苷和沙伐林iv联合用于缓解更年期症状的有效性

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-08 DOI: 10.1016/j.talo.2025.100552

Rithika Naveencharan , Ashwin Sivakumar , Rishi Senthil Kumar , Sheena Christabel Pravin , Reena Monica P , V. Kiruthika , Abhijeet Morde , Arun Balakrishnan , Muralidhara Padigaru , Ninad Puranik , Ravindra Nayakwadi , Tejas Namjoshi

Shatavarin-IV and Protodioscin, saponins of Asparagus racemosus and Trigonella foenum-graecum respectively are phytoestrogens commonly used to alleviate post-menopausal symptoms such as hot flashes, sleeping disorder, and mood sways. While their individual benefits are well-documented, the synergistic effects of these compounds remain underexplored. This study aims to validate the combination of Shatavarin-IV and Protodioscin on molecular mediators of menopausal symptoms using advanced computational analyses. Deep learning model for drug additivity analysis was developed and trained on the DrugComb database using the Loewe’s additivity score as the target variable, with a focus on combinations tested on the MCF7 cell line and validated of the 786-O cell line. Molecular docking simulations were performed using PyRx, PyMOL, FPocket and ChimeraX to investigate the binding affinities of Protodioscin and Shatavarin-IV with key neuronal modulators of hot flashes, including Kisspeptin, Neurokinin B, TRPV1, c-FOS, and GnRH. IC50 values were derived from binding free energies. Dose-response simulations were evaluated across concentrations (0.01 µM to 100 µM) using a four-parameter logistic equation. Absorption, Distribution, Metabolism, and Elimination (ADME) profiling was determined to predict bioavailability and toxicity. The dose-response simulations confirmed significant therapeutic potential at relevant concentrations and additive interaction between both the compounds suggests enhanced therapeutic efficacy. ADME profiling indicated favorable bioavailability, non-toxicity, and efficient metabolism for both compounds and provides novel insights into their molecular mechanism of action in the body. The results of this research indicate that the combined additive effect of Protodioscin and Shatavarin-IV in alleviating menopausal symptoms exhibits favorable pharmacokinetic profile, and could potentially be promising candidates for future human clinical studies.

总状芦笋皂苷和三角芦笋皂苷原薯蓣皂苷分别是植物雌激素，常用于缓解绝经后潮热、睡眠障碍、情绪波动等症状。虽然它们各自的益处已被充分证明，但这些化合物的协同效应仍未得到充分探讨。本研究旨在通过先进的计算分析验证Shatavarin-IV和原薯蓣皂苷对绝经期症状分子介质的联合作用。以Loewe加性评分为目标变量，在DrugComb数据库上开发并训练了药物加性分析的深度学习模型，重点对MCF7细胞系和786-O细胞系进行了组合测试和验证。利用PyRx、PyMOL、FPocket和ChimeraX进行分子对接模拟，研究Protodioscin和Shatavarin-IV与热潮热的关键神经元调节剂Kisspeptin、Neurokinin B、TRPV1、c-FOS和GnRH的结合亲和力。IC50值由束缚自由能导出。使用四参数logistic方程评估不同浓度（0.01µM至100µM）的剂量-反应模拟。吸收、分布、代谢和消除（ADME）分析被确定预测生物利用度和毒性。剂量-反应模拟证实了在相关浓度下显著的治疗潜力，两种化合物之间的加性相互作用表明治疗效果增强。ADME分析表明，这两种化合物具有良好的生物利用度、无毒性和有效的代谢，并为其在体内的分子作用机制提供了新的见解。本研究结果表明，原diooscin和Shatavarin-IV在缓解绝经期症状方面的联合加性作用表现出良好的药代动力学特征，可能是未来人类临床研究的潜在有希望的候选者。

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引用次数: 0

Thorium determination in seawater by inductively coupled plasma mass spectrometry: Method development and oceanographic applications 电感耦合等离子体质谱法测定海水中的钍：方法发展和海洋学应用

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-08 DOI: 10.1016/j.talo.2025.100553

Yingjie Zou , Peng Wang , Lei Xu , Hong Ren , Yiyao Cao

Thorium isotopes in the ocean (including radiogenic isotopes and terrigenous-derived ²³²Th) serve as key tracers for studying land-derived material transport. While high-sensitivity and high-resolution inductively coupled plasma mass spectrometry (ICP-MS) has become the mainstream technique for seawater thorium isotope analysis, its application faces three major challenges: complex pretreatment purification procedures, varying sample volume requirements, and significant instrument memory effects. This review systematically summarizes the technical difficulties and solutions in ICP-MS-based thorium isotope detection and examines its oceanographic applications in quantifying dust inputs and tracing water mass mixing, thereby providing critical data for assessing marine carbon pump efficiency.

海洋中的钍同位素（包括放射性同位素和陆源源的232Th）是研究陆源物质运输的关键示踪剂。虽然高灵敏度、高分辨率电感耦合等离子体质谱（ICP-MS）已成为海水钍同位素分析的主流技术，但其应用面临三大挑战：复杂的预处理纯化程序、不同的样品体积要求和显著的仪器记忆效应。本文系统总结了基于icp - ms的钍同位素检测的技术难点和解决方案，并探讨了其在定量粉尘输入和跟踪水团混合方面的海洋应用，从而为评估海洋碳泵效率提供关键数据。

引用次数: 0

Renewable carbon from flax shives with silver nanoparticles biosynthesized using Eichhornia crassipes extract for green electrochemical detection of hydroxychloroquine 以亚麻籽提取物为原料合成可再生碳纳米银用于绿色电化学检测羟氯喹

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-05 DOI: 10.1016/j.talo.2025.100548

Francisco Contini Barreto , Gloria Tersis Vieira dos Santos , Maria Eduarda Barberis , Naelle Kita Mounienguet , Martin Kássio Leme da Silva , Quan He , Ivana Cesarino

Hydroxychloroquine (HCQ) is an antimalarial drug that was repurposed during the COVID-19 pandemic. However, due to its limited clinical efficacy and notable side effects, its recommendation was later withdrawn. Despite this, HCQ sales increased by over 800 % in Brazil, and its presence has since been detected in aquatic environments, raising concerns regarding potential risks to both environmental and human health. In response, a novel green electrochemical sensor was developed for the determination of HCQ in water samples. The sensor is based on a glassy carbon electrode modified with hydrochar derived from flax shives and silver nanoparticles biosynthesized using Eichhornia crassipes (water hyacinth) extract as a reducing and stabilizing agent (GC/HC-AgNPs). The sensor was characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and cyclic voltammetry (CV). Experimental parameters were optimized, and the device was successfully applied for the detection of HCQ using differential pulse voltammetry (DPV). The sensor exhibited a linear response in the range of 1.0–40.0 µmol L⁻¹, with a limit of detection (LOD) of 0.265 µmol L⁻¹ and a limit of quantification (LOQ) of 0.885 µmol L⁻¹. Excellent repeatability (RSD = 2.27 %) and reproducibility (RSD = 5.75 %) were achieved. Application in tap and lake water samples resulted in recovery values ranging from 89.5 % to 105.0 %, and comparative analysis with UV–vis spectroscopy confirmed the accuracy of the proposed method. The sensor further demonstrated good selectivity even in the presence of relevant interfering species. Green chemistry assessment tools confirmed the sustainable character of the developed method. Overall, the proposed sensor represents a promising, effective, and environmentally friendly platform for HCQ monitoring in water matrices.

羟氯喹（HCQ）是一种抗疟疾药物，在COVID-19大流行期间被重新利用。然而，由于其临床疗效有限，副作用明显，后来撤销了推荐。尽管如此，HCQ在巴西的销量增长了800%以上，此后在水生环境中发现了HCQ的存在，引发了人们对环境和人类健康潜在风险的担忧。为此，研制了一种新型绿色电化学传感器，用于水样中HCQ的测定。该传感器基于一种玻璃碳电极，该电极由亚麻屑中提取的碳氢化合物修饰，而银纳米粒子是用水葫芦提取物作为还原剂和稳定剂（GC/HC-AgNPs）生物合成的。采用扫描电子显微镜（SEM）、能量色散x射线能谱（EDS）和循环伏安法（CV）对传感器进行了表征。对实验参数进行了优化，成功地将该装置应用于差分脉冲伏安法（DPV）检测HCQ。该传感器在1.0-40.0µmol L⁻¹范围内呈线性反应，检测限为0.265µmol L⁻¹，定量限为0.885µmol L⁻¹。重复性好（RSD = 2.27%），重现性好（RSD = 5.75%）。应用于自来水和湖泊水样，回收率为89.5% ~ 105.0%，与紫外-可见光谱对比分析证实了该方法的准确性。该传感器在相关干扰物质存在的情况下也表现出良好的选择性。绿色化学评价工具证实了所开发方法的可持续性。总的来说，所提出的传感器代表了一个有前途的、有效的、环保的平台，用于监测水基质中的HCQ。

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