Talanta Open最新文献

{"title":"Bone fragility in Type 2 Diabetes Mellitus. Influence of sex and cardiovascular disease in a pilot study using metabolomics","authors":"Nicolás Redecilla-Montoya ,&nbsp;Cristina García-Fontana ,&nbsp;Tomás Clive Barker-Tejeda ,&nbsp;Andrea Macías-Camero ,&nbsp;Francisco Andújar-Vera ,&nbsp;María Fernanda Rey-Stolle ,&nbsp;Luis Martínez-Heredia ,&nbsp;Iván Iglesias-Baena ,&nbsp;Ana Gradillas ,&nbsp;Coral Barbas ,&nbsp;Beatriz García-Fontana ,&nbsp;Manuel Muñoz-Torres ,&nbsp;Alma Villaseñor","doi":"10.1016/j.talo.2024.100381","DOIUrl":"10.1016/j.talo.2024.100381","url":null,"abstract":"<div><div>Type 2 diabetes mellitus (T2DM) is one of the most common worldwide metabolic disorders, characterized by insulin resistance (IR) and defective insulin secretion by pancreatic β-cells which leads to multiple complications such as bone fragility. This complication might be influenced by other factors such as gender and cardiovascular disease (CVD). However, it is unclear why a certain T2DM group develops bone fragility, and what the molecular mechanism is. Metabolomics is a powerful approach to the study of human metabolism, especially in complex diseases such as T2DM. Thus, this study aimed to identify significant metabolites associated with bone fragility in T2DM patients. To achieve this, 81 individuals were enrolled and classified as T2DM patients (D, n=28), T2DM with bone fragility (D-Frag, n=25), or age-matched non-diabetic subjects (ND, n=28) as the control group. Serum samples were collected and analyzed using liquid chromatography and gas chromatography, both coupled to mass spectrometry (LC-MS and GC-MS, respectively). Samples were compared within four different scenarios: 1) the classical comparison of <strong>D <em>vs</em> ND</strong> to corroborate previous studies; 2) <strong>D-Frag <em>vs</em> D</strong> to explore the metabolites mainly associated with bone fragility; 3) the same comparison using male data (<strong>MD-Frag <em>vs</em> MD</strong>) to study as a more homogeneous model of bone fragility as in women, bone fragility could be mainly associated with hormonal stage and pregnancy; and 4) <strong>MD-Frag-CVD <em>vs</em> MD-CVD</strong> to explore the influence of bone fragility in the male-based model with CVD considering that most of the T2DM patients suffer from CVD. After analysis of these scenarios, our results suggest that acylcarnitines and glycerophospholipids, among other metabolites, are involved in the development of bone fragility in T2DM.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100381"},"PeriodicalIF":4.1,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical–chemical characterization of purified phenol red for spectrophotometric pH measurements in riverine, estuarine, and oceanic waters","authors":"Kalla L. Fleger,&nbsp;Robert H. Byrne,&nbsp;Xuewu Liu","doi":"10.1016/j.talo.2024.100380","DOIUrl":"10.1016/j.talo.2024.100380","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Phenol red (PR) is one of several sulfonephthalein indicators used to provide rapid and precise spectrophotometric pH measurements of seawater and similar solutions. With an approximate pH-indicating range of 5.9 to 7.7, this dye is well suited to fill a critical gap in spectrophotometric pH-measurement capabilities – e.g., the slightly acidic waters of environments low in oxygen or high in carbon dioxide. For highest-quality measurements, the salinity and temperature dependence of indicator behavior must be established, but previous characterizations of PR were for impure indicator powder or for low-salinity solutions only. This work is the first to comprehensively characterize purified phenol red over wide ranges of temperature (&lt;em&gt;T&lt;/em&gt;; absolute temperature in K) and salinity (&lt;em&gt;S&lt;/em&gt;&lt;sub&gt;P&lt;/sub&gt;; practical scale). Measurements of spectrophotometric pH&lt;sub&gt;T&lt;/sub&gt; (total hydrogen ion concentration scale) are given by:&lt;span&gt;&lt;span&gt;&lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mtext&gt;pH&lt;/mtext&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;/msub&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;mtext&gt;log&lt;/mtext&gt;&lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt;&lt;msubsup&gt;&lt;mi&gt;K&lt;/mi&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;/msubsup&gt;&lt;msub&gt;&lt;mi&gt;e&lt;/mi&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;mo&gt;)&lt;/mo&gt;&lt;/mrow&gt;&lt;mo&gt;+&lt;/mo&gt;&lt;mtext&gt;log&lt;/mtext&gt;&lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt;&lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt;&lt;mi&gt;R&lt;/mi&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;msub&gt;&lt;mi&gt;e&lt;/mi&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;/msub&gt;&lt;mo&gt;)&lt;/mo&gt;&lt;/mrow&gt;&lt;mo&gt;/&lt;/mo&gt;&lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;mi&gt;R&lt;/mi&gt;&lt;msub&gt;&lt;mi&gt;e&lt;/mi&gt;&lt;mn&gt;4&lt;/mn&gt;&lt;/msub&gt;&lt;mo&gt;)&lt;/mo&gt;&lt;/mrow&gt;&lt;mo&gt;)&lt;/mo&gt;&lt;/mrow&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;where &lt;em&gt;K&lt;/em&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;sup&gt;T&lt;/sup&gt; is the second dissociation constant of fully protonated PR, and &lt;em&gt;e&lt;/em&gt;&lt;sub&gt;1&lt;/sub&gt;, &lt;em&gt;e&lt;/em&gt;&lt;sub&gt;2&lt;/sub&gt;, and &lt;em&gt;e&lt;/em&gt;&lt;sub&gt;4&lt;/sub&gt; are PR molar absorption coefficient ratios. The term &lt;em&gt;R&lt;/em&gt; is the ratio of absorbances measured in the sample of interest at 558 and 433 nm. In this work, we derived a simplified method for determining the parameter &lt;em&gt;e&lt;/em&gt;&lt;sub&gt;1&lt;/sub&gt; of any sulfonephthalein indicator and also fully characterized PR physical–chemical characteristics for 275.15 ≤ &lt;em&gt;T&lt;/em&gt; ≤ 308.15 K and 0 ≤ &lt;em&gt;S&lt;/em&gt;&lt;sub&gt;P&lt;/sub&gt; ≤ 40, yielding:&lt;span&gt;&lt;span&gt;&lt;span&gt;&lt;math&gt;&lt;mtable&gt;&lt;mtr&gt;&lt;mtd&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;e&lt;/mi&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;/msub&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;mn&gt;2.12261&lt;/mn&gt;&lt;mo&gt;×&lt;/mo&gt;&lt;msup&gt;&lt;mn&gt;10&lt;/mn&gt;&lt;mrow&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;/mrow&gt;&lt;/msup&gt;&lt;mo&gt;+&lt;/mo&gt;&lt;mn&gt;1.37448&lt;/mn&gt;&lt;mo&gt;×&lt;/mo&gt;&lt;msup&gt;&lt;mn&gt;10&lt;/mn&gt;&lt;mrow&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;mn&gt;5&lt;/mn&gt;&lt;/mrow&gt;&lt;/msup&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mo&gt;+&lt;/mo&gt;&lt;mn&gt;3.061&lt;/mn&gt;&lt;mo&gt;×&lt;/mo&gt;&lt;msup&gt;&lt;mn&gt;10&lt;/mn&gt;&lt;mrow&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;mn&gt;10&lt;/mn&gt;&lt;/mrow&gt;&lt;/msup&gt;&lt;msubsup&gt;&lt;mi&gt;S&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;P&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow&gt;&lt;mn&gt;0.5&lt;/mn&gt;&lt;/mrow&gt;&lt;/msubsup&gt;&lt;msup&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msup&gt;&lt;/mrow&gt;&lt;/mtd&gt;&lt;/mtr&gt;&lt;mtr&gt;&lt;mtd&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;e&lt;/mi&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mn&gt;3.6429426&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;mn&gt;2.8139&lt;/mn&gt;&lt;mo&gt;×&lt;/mo&gt;&lt;msup&gt;&lt;mn&gt;10&lt;/mn&gt;&lt;mrow&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;/mrow&gt;&lt;/msup&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;/mrow&gt;&lt;/mtd&gt;&lt;/mtr&gt;&lt;mtr&gt;&lt;mtd&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;e&lt;/mi&gt;&lt;mn&gt;4&lt;/mn&gt;&lt;/msub&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mn&gt;8.0884775&lt;/mn&gt;&lt;mo&gt;×&lt;/mo&gt;&lt;msup&gt;&lt;mn&gt;10&lt;/mn&gt;&lt;mrow&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/mrow&gt;&lt;/msup&gt;&lt;mo&gt;+&lt;/mo&gt;&lt;mn&gt;6.2187&lt;/mn&gt;&lt;mo&gt;×&lt;/mo&gt;&lt;msup&gt;&lt;mn&gt;10&lt;/mn&gt;&lt;mrow&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;mn&gt;5&lt;/mn&gt;&lt;/mrow","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100380"},"PeriodicalIF":4.1,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecularly imprinted biomimetic plasmonic sensor decorated with gold nanoparticles for selective and sensitive detection of bisphenol A","authors":"Ilgım Göktürk ,&nbsp;Süleyman Aşır ,&nbsp;Deniz Türkmen ,&nbsp;Adil Denizli ,&nbsp;Fatma Yılmaz","doi":"10.1016/j.talo.2024.100379","DOIUrl":"10.1016/j.talo.2024.100379","url":null,"abstract":"<div><div>Molecularly imprinted polymers inspired by antigen-antibody interactions have received substantial interest as a biomimetic artificial receptor system in environmental applications. Herein, we present a molecularly imprinted surface plasmon resonance sensor integrated with gold nanoparticles for the identification of bisphenol A (BPA), an endocrine-disrupting chemical. We synthesized BPA-imprinted nanofilm consisting of amino acid-based functional monomers to selectively detect BPA from synthetic wastewater samples. BPA-spiked synthetic wastewater samples were analyzed to ensure the method's reliability and feasibility. Under ideal conditions, the suggested approach performed well in terms of analytical performance to bisphenol A, with a wide linear range of 0.1 to 10 µg/L and LOD of 10 ng/L. The sensor results align well with the Langmuir adsorption model. It has also been shown that repeated use of the sensor can be achieved. According to selectivity studies, bisphenol A adsorbed within the imprinted cavities favorably compared to 4-nitrophenol and phenol. The produced bisphenol A-imprinted surface plasmon resonance sensor provides improved sensitivity based on the signal amplification strategy, unconjugated sensing without the need for labelling, real-time sensing, low sample consumption rates, quantifiable assessment, and outstanding kinetic rate constant calculation in actual samples. Also, because the produced sensor is reusable with relative standard deviations (RSD)&lt;1.25, indicating the sensor's precision, the surface plasmon resonance-based biomimetic BPA sensor is simple to practice and a cost-effective option.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100379"},"PeriodicalIF":4.1,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-burn and post-blast rapid detection of trace and bulk energetics by 3D-printed cone spray ionization mass spectrometry","authors":"Ryan M. Bain ,&nbsp;TJ Pinedo ,&nbsp;Doug J. Klapec ,&nbsp;Christopher C. Mulligan ,&nbsp;Patrick W. Fedick","doi":"10.1016/j.talo.2024.100377","DOIUrl":"10.1016/j.talo.2024.100377","url":null,"abstract":"<div><div>Forensic analysis of post-blast and post-burn materials can be critical to a law enforcement or military investigation. 3D-printed cone spray ionization (3D-PCSI) coupled to a field portable mass spectrometer (MS) has been demonstrated as a rapid and low-cost alternative to traditional laboratory-based techniques (high resolution liquid or gas chromatography mass spectrometry) for the detection and analysis of energetic materials. 3D-PCSI-MS can sample trace or bulk energetics within or upon solid matrices with no sample preparation. Solid samples, in this case post-blast or post-burn energetics, are placed within the hollow cavity of the cone that has an approximately 100 µm spray-based emitter at the apex. An extraction/spray solvent is added, and a high voltage is subsequently applied. When coupled to a field portable MS, post-burn and post-blast analyses can be performed in the field. Double base propellant was burned on a variety of substrates and examined via 3D-PCSI-MS. An energetic material and stabilizers used in propellants were detected from the post-burn samples with no sample preparation. Trinitrotoluene (TNT, used in military and commercial explosives) and triacetone triperoxide (TATP, a homemade explosive) were examined post-blast on the US ton scale and the gram scale, respectively.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100377"},"PeriodicalIF":4.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of engineered Zn-MOF/g-C3N4 based photoelectrochemical system for real-time sensors and removal of naproxen in wastewater","authors":"Abera D. Ambaye ,&nbsp;Sithembela A. Zikalala ,&nbsp;Karabo C. Mashiloane ,&nbsp;Jemal F. Nure ,&nbsp;Mesfin A. Kebede ,&nbsp;Touhami Mokrani ,&nbsp;Edward N. Nxumalo","doi":"10.1016/j.talo.2024.100371","DOIUrl":"10.1016/j.talo.2024.100371","url":null,"abstract":"<div><div>Naproxen-contaminated water may lead to the accumulation of the drug in aquatic organisms and can pose high risks to an aquatic environment and human beings. Therefore, this work aimed to develop photoelectrochemical sensing and degradation of naproxen (NPX) using zinc-metal organic framework /graphitic carbon nitride thin film-based fluorine-doped tin oxide (Zn-MOF/g-C₃N₄/FTO) as anode material for the sensing and degradation of naproxen (NPX). The surface morphology, structure, surface property, surface area, optical property, photocurrent, and charge transfer kinetics abilities were studied using different techniques. The nanocomposites showed a superior photocurrent response (0.815 mA cm^-2) compared to the original g-C₃N₄ (0.328 mA cm^-2). The photo-anode made of Zn-MOF@g-C₃N₄/FTO displayed the highest photocurrent value, indicating that the alignment of the two semiconductor bands prevented the quick recombination of electron-hole pairs. Owing to these attractive features, the Zn-MOF/g-C₃N₄/FTO electrode was applied for photoelectrochemical detection of NPX using chronoamperometry. Interestingly, the nanocomposites-based FTO ascribed a lower detection limit (2.3 ng <span>l</span>^-1) with a wide linear range concentration of NPX (0.5 to 200 µg <span>l</span>^-1). Additionally, the analytical assessment of repeatability and reproducibility demonstrated robust performance, with commendable relative standard deviations (RSD%) of 2.54 % and 2.40 %, respectively. On the other hand, a remarkable degradation efficiency of 97.52 % was attained when employing a bias potential of 0.1 V during a 2 h photoelectrocatalytic oxidation of NPX. The degradation process was primarily driven by the active participation of holes and hydroxyl radicals in ring opening and subsequent cleavage of by-products. The notable effectiveness of this degradation can be attributed to the combined and synergistic effects of both electrochemical and photocatalytic degradation techniques. The current state demonstrates its effectiveness in the photoelectrochemical sensing and removal of NPX using MOF/g-C₃N₄ nanocomposites-based electrode materials in wastewater.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100371"},"PeriodicalIF":4.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Continuous-flow analysis of nitrogen compounds in environmental water using a copper–zinc reduction coil","authors":"Satoshi Morikubo ,&nbsp;Nodoka Takahashi ,&nbsp;Yorihiro Kumazawa ,&nbsp;Yasuhiko Takuma ,&nbsp;Daisuke Enomoto ,&nbsp;Takashi Nishimura ,&nbsp;Ryosei Kanno ,&nbsp;Suguru Okunishi ,&nbsp;Hiroto Maeda","doi":"10.1016/j.talo.2024.100378","DOIUrl":"10.1016/j.talo.2024.100378","url":null,"abstract":"<div><div>The copper–cadmium reduction method has been applied to determine nitrogen compounds in heavily contaminated environmental water. However, there is an international demand to reduce the use of cadmium because it is harmful to humans and environment. Hence, a copper–zinc reduction continuous flow analysis system without using cadmium was developed for nitrogen-compound determination. However, the effects of seawater contaminants on this analytical method remains unknown. Therefore, in this study, the effects of contaminants on the developed method are investigated, and the applicability of the method to environmental water analysis is examined. Resultantly, interference due to magnesium precipitation is confirmed; however, this could be prevented by adjusting the concentration of the analytical reagent, i.e.,. ethylenediaminetetraacetic acid disodium. The measurement of certified reference materials under the new conditions, with precipitation countermeasures, show good results, with an accuracy of over 96 %. Additionally, a recovery study using actual environmental water afforded good results, with recovery of &gt;95 % for all samples. These results indicate that the proposed method is an excellent, cadmium-free analytical method capable of analyzing environmental water as effectively as conventional methods.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100378"},"PeriodicalIF":4.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing isomer specificity in mass spectrometry by combining silver ion adduction and ion mobility","authors":"Varun V. Sharma ,&nbsp;Danjo De Chavez ,&nbsp;Susan E. Slade ,&nbsp;Ingela Lanekoff","doi":"10.1016/j.talo.2024.100373","DOIUrl":"10.1016/j.talo.2024.100373","url":null,"abstract":"<div><h3>Background</h3><div>Identification and characterization of steroids from complex mixtures with isomeric precision is key to studying endocrine-related metabolism and disorders. Whereas the golden standard chromatography, including liquid chromatography and gas chromatography, can be coupled with mass spectrometry to separate steroids prior to ionization, this separation is time-consuming. Contrarily, direct infusion techniques can offer increased throughput; however, these are often hampered by limited structural specificity. Thus, it is important to develop new analytical tools for direct infusion mass spectrometry that will provide isomeric specificity.</div></div><div><h3>Results</h3><div>Herein, we show that direct infusion with electrospray ionization in combination with silver adduction and cyclic ion mobility mass spectrometry (cIMS) enables mobility separation and improves the detectability of steroid isomers. Specifically, silver ion adduction of steroids increases instrumental response up to 14 times and enables almost baseline mobility separation of closely related structural steroid isomers even at low cIMS resolution. By combining experimental and theoretical data, we show that the silver interacts with the steroid isomer at single or multiple sites, which introduces conformational changes that enable mobility separation. Moreover, we show that the combination of cIMS and silver adduct fragmentation in tandem mass spectrometry provides an additional dimension for annotation of steroid isomers. Thus, the simple introduction of silver ions into the electrospray solvent provides a great gain in the analytical discernment of steroid isomers.</div></div><div><h3>Significance</h3><div>For the first time, we show that the use of silver adduction introduces a conformational change in steroids that allows for them to be separated with low-resolution ion mobility spectrometry without any prior derivatization, chromatographic separation, or instrumental modification. This is a new and important tool for analyzing steroid isomers that can unravel their importance in biological systems.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100373"},"PeriodicalIF":4.1,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trace-level quantification of NDMA in levosulpuride active pharmaceutical ingredient and tablet formulation Using UFLC-MS/MS","authors":"Hemanth Vikram P․R ,&nbsp;Gunjan Kumar ,&nbsp;Rajashree Deka ,&nbsp;Umme Hani ,&nbsp;Nazima Haider ,&nbsp;Sirajunisa Talath ,&nbsp;Adil Farooq Wali ,&nbsp;Dilipkumar Reddy Kandula ,&nbsp;Narasimha M. Beeraka ,&nbsp;Sinchana B Gopalaiah ,&nbsp;Devi Sri Chiriki ,&nbsp;Namitha Bannimath ,&nbsp;Pramod Kumar ,&nbsp;Bannimath Gurupadayya","doi":"10.1016/j.talo.2024.100375","DOIUrl":"10.1016/j.talo.2024.100375","url":null,"abstract":"<div><div>Nitrosamine impurities identified in several pharmaceuticals during recent times has raised concerns leading to product recalls worldwide and necessitating sensitive liquid and gas chromatographic methods for trace level detection of nitrosamine impurities. This study developed and validated a ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method for the quantification of NDMA in Levosulpuride drug substance and tablet formulation. Current method utilizes a triple quadrupole analyzer, atmospheric pressure chemical ionization (APCI) ionization source and multiple reaction monitoring (MRM) scan mode for the analysis. Chromatographic separation was achieved on a Gemini NX-C18 column (150 × 4.6 mm, 3 µm) maintained at 40 °C. The mobile phase consisted of a binary gradient of solvent A (0.1 % formic acid in water) and solvent B (methanol), with a total run time of 18 minutes. Current method achieved excellent linearity, recovery, precision, and sensitivity. Greenness of the developed method was evaluated using the GAPI, AGREE, and AES metrics. Current method is sensitive and selective for NDMA in levosulpuride drug substance and tablet formulations and can be employed for routine quality control analysis in pharmaceutical industry.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100375"},"PeriodicalIF":4.1,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142592729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of nanomaterial-supported molecularly imprinted polymer/receptor-like sensor for the detection of rosuvastatin from binary mixtures","authors":"Mahdi Gharibi ,&nbsp;Ensar Piskin ,&nbsp;Nadia Bounoua ,&nbsp;Ahmet Cetinkaya ,&nbsp;Sibel A. Ozkan","doi":"10.1016/j.talo.2024.100376","DOIUrl":"10.1016/j.talo.2024.100376","url":null,"abstract":"<div><div>Statins reduce cholesterol synthesis by inhibiting the enzyme hydroxymethylglutaryl-CoA (HMG-CoA) reductase. Rosuvastatin (ROS) is a statin drug used to prevent cardiovascular diseases and treat abnormal lipids. This study describes the design and fabrication of a nanomaterial-assisted molecularly imprinted polymer (MIP)-based electrochemical sensor for ROS determination. In addition, MIP-based electrochemical sensors were designed to detect ROS in both standard solutions and serum samples. In the MIP-based electrochemical sensor developed via the photopolymerization (PP) technique on the glassy carbon electrode (GCE) surface, were used zinc oxide nanoparticles (ZnO NPs) as a pore former, p-aminobenzoic acid (p-ABA) as functional monomer, ROS as template molecule. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and scanning electron microscopy (SEM) techniques were used to carry out detailed characterizations concerning surface morphology and electrochemistry. Under optimized experimental conditions, the linearity range of the designed sensor was found 1.0 × 10⁻¹³–1.0 × 10⁻¹² M. The superior selectivity of the MIP-based sensor against ROS in the presence of their binary mixtures was confirmed by interference studies. The recovery rates of the MIP-based sensors were calculated as 100.35 % and 99.16 % in commercial tablet form and serum samples, respectively. Moreover, the proposed sensor's relative selectivity coefficient (k′) was calculated, and it provided good selectivity for ROS over the NIP sensor. In conclusion, this newly developed sensor offers an advantageous approach for selective, sensitive, rapid and cost-effective analysis of ROS from binary mixtures. Additionally, this study is the first electrochemical sensor using nanomaterial-assisted MIP technology for ROS analysis, and its sensitivity is higher than that of other studies in the literature.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100376"},"PeriodicalIF":4.1,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142592728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal organic frameworks for bacterial detection in environmental samples","authors":"Hadeer M. Bedair ,&nbsp;Alaa Bedair ,&nbsp;Mahmoud Hamed ,&nbsp;Marcello Locatelli ,&nbsp;Fotouh R. Mansour","doi":"10.1016/j.talo.2024.100374","DOIUrl":"10.1016/j.talo.2024.100374","url":null,"abstract":"<div><div>Pathogenic bacteria pose significant threats to public health. Early and accurate detection of bacteria is crucial for infection control and prevention of outbreaks. This paper reviews recent advances in metal organic framework (MOF)-based methods for bacterial detection. MOFs are promising materials for biosensing due to their large surface areas, customizable properties, and ability to integrate various sensing capabilities. Several colorimetric and electrochemical detection techniques that utilize MOFs are discussed. Colorimetric assays combining MOFs with gold nanoparticles, peroxidase-mimicking activity, and aptamer recognition have achieved sensitive and specific detection of bacteria such as <em>E. coli</em> and <em>S. aureus</em>. Electrochemical biosensors integrating MOFs, antibodies, aptamers and quantum dots have also demonstrated low detection limits for various bacteria. Notably, techniques using MOF peroxidase-like activity coupled with magnetic separation or competitive binding assays show potential for point-of-care pathogen detection. Furthermore, optimization of MOF properties and integration within microfluidic platforms may lead to portable, low-cost and rapid methods suitable for on-site bacterial analysis in diverse settings. Continued exploration of MOF-based sensing strategies holds promise for improved bacteria monitoring and control of infectious diseases.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100374"},"PeriodicalIF":4.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}