Talanta Open最新文献_第5页

Simultaneous quantification and natural 13C abundance of fatty acids in breast cancer tissues and serum by GC-C-IRMS for tumor characterization GC-C-IRMS同时定量测定乳腺癌组织和血清中脂肪酸的天然13C丰度，用于肿瘤表征

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-10 DOI: 10.1016/j.talo.2025.100573

Louise Mangeon , Romain Le Balch , Olivier L. Mantha , Cyrille Guimaraes-carneiro , Michelle Pinault , Régis Hankard , Arnaud De Luca , Illa Tea

Background

Breast cancer (BrCa) is a heterogeneous disease which complicates early detection, subtyping, and treatment selection. Metabolic alterations are hallmarks of cancer, but current methods to study metabolism, especially stable isotope analysis, involve complex workflows, derivatization steps, and have limited clinical use. Lipid metabolism is altered in BrCa, yet sensitive, minimally invasive tools to quantify fatty acid (FA) isotope signatures are lacking. A gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) method allowing direct, high-precision analysis of underivatized FAs in tissues and serum is needed for improved cancer phenotyping.

Results

A novel GC-C-IRMS method is developed for direct δ¹³C (carbon isotope composition) and concentration analysis of underivatized FAs in tissues and serum, with excellent repeatability, reproducibility, and minimal matrix effects. The optimized method involves simple sample preparation by lipid hydrolysis without requiring derivatization, followed by FA separation using a polar GC column. The method is validated for both quantification and isotopic analysis using internal standards and applied to human tissue and serum samples from patients with different BrCa subtypes. Significant natural ¹³C enrichment is observed in C16:0, C16:1, and C18:1, alongside a decrease in C14:0 and C16:1 concentrations in cancerous tissue compared to adjacent non-cancerous tissue, reflecting shifts in lipid metabolism during carinogenesis. Importantly, δ¹³C values of C18:0 and C18:2 in both cancerous tissue and serum differed betwen BrCa subtypes.

Significance

This is the first GC-C-IRMS method enabling high-precision, direct analysis of underivatized FAs in tissues and serum with minimal sample preparation. The approach offers a promising, minimally invasive tool for characterizing FA metabolism and improving breast cancer detection, subtyping, and metabolic phenotyping without isotopic labeling.

乳腺癌（BrCa）是一种异质性疾病，它使早期发现、亚型分型和治疗选择变得复杂。代谢改变是癌症的标志，但目前研究代谢的方法，特别是稳定同位素分析，涉及复杂的工作流程，衍生化步骤，并且临床应用有限。脂质代谢在BrCa中发生改变，但缺乏敏感的、微创的工具来量化脂肪酸（FA）同位素特征。气相色谱-燃烧-同位素比值质谱（GC-C-IRMS）方法可以直接，高精度地分析组织和血清中未活化的FAs，这是改善癌症表型所必需的。结果建立了一种新的GC-C-IRMS方法，用于组织和血清中欠活化FAs的δ13C（碳同位素组成）和浓度的直接分析，具有良好的重复性、再现性和最小的基质效应。优化后的方法包括通过脂质水解制备样品，无需衍生化，然后使用极性气相色谱柱分离FA。使用内部标准验证了该方法的定量和同位素分析，并应用于不同BrCa亚型患者的人体组织和血清样本。在C16:0、C16:1和C18:1中观察到显著的天然13C富集，同时与邻近非癌组织相比，癌组织中C14:0和C16:1浓度降低，反映了癌变过程中脂质代谢的变化。重要的是，癌组织和血清中C18:0和C18:2的δ¹³C值在BrCa亚型之间存在差异。这是第一个GC-C-IRMS方法，可以高精度，直接分析组织和血清中未酸化的FAs，只需最少的样品制备。该方法提供了一种有前途的微创工具，用于表征FA代谢，改善乳腺癌检测、亚型分型和代谢表型，而无需同位素标记。

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引用次数: 0

A novel sampling strategy for metal ions determination in water: application to the determination of cadmium 水中金属离子测定的新取样策略：在镉测定中的应用

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-08 DOI: 10.1016/j.talo.2025.100574

Francisco A.P. Rodrigues, Tânia C.F. Ribas, Raquel B.R. Mesquita, António O.S.S. Rangel

This work proposes a novel, portable, and reusable device for in situ water sampling, sample preparation, and analyte enrichment in a single step. For this purpose, a commercial cationic exchange resin (Chelex 100), was used to obtain a solid-phase extraction column for cadmium (II) retention and enrichment. The optimization of key parameters such as sample volume, reagents concentration, and flow-rate, was performed to maximize cadmium retention and elution efficiency. The method was applied to real samples, and the results agreed with those obtained with reference methodologies, with no significant differences (< 10%). Additionally, recovery rates of 97.8 ± 3.5% were achieved. This method integrates, in one step, the sampling and sample preparation procedures and employs reusable devices, contributing to providing a sustainable solution for metal ions monitoring in aquatic environments.

这项工作提出了一种新的，便携式的，可重复使用的装置，用于原位水采样，样品制备和分析物富集在一个步骤中。为此，使用商业阳离子交换树脂（Chelex 100）获得镉（II）保留和富集的固相萃取柱。对样品体积、试剂浓度和流速等关键参数进行了优化，以最大限度地提高镉的保留和洗脱效率。将该方法应用于实际样品，结果与参考方法一致，无显著差异（< 10%）。回收率为97.8±3.5%。该方法将采样和样品制备程序整合在一起，采用可重复使用的设备，有助于为水生环境中的金属离子监测提供可持续的解决方案。

引用次数: 0

Analytical techniques and source apportionment for heavy metal(loid)s in groundwater: A comprehensive review 地下水中重金属（样态）分析技术及来源分析综述

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-02 DOI: 10.1016/j.talo.2025.100572

Jelena Vesković, Antonije Onjia

Groundwater contamination by heavy metal(loid)(HM) is a pervasive global issue threatening drinking water quality and public health. Effective mitigation requires robust analytical methodologies and predictive modeling frameworks. This review critically assesses cutting-edge instrumental techniques and source apportionment methods for detecting and understanding HM pollution in groundwater. Advanced laboratory techniques, exemplified by inductively coupled plasma mass spectrometry (ICP-MS), now achieve extraordinary sensitivity with detection limits in the sub-μg/L range and exhibit high precision (relative standard deviations between 2–3 %). Furthermore, hyphenated chromatography-spectrometry techniques, such as ion chromatography coupled to ICP-MS, facilitate detailed chemical speciation, effectively resolving redox-sensitive pairs such as Cr(III)/Cr(VI) and As(III)/As(V) at environmental concentrations (1–50 µg/L). Meanwhile, emerging electrochemical portable sensors enable on-site, real-time monitoring of groundwater quality. For source apportionment, multivariate statistical methods (including receptor models), isotopic fingerprinting, and machine learning algorithms have been increasingly integrated to disentangle complex mixtures of natural versus anthropogenic metal sources. By combining improved detection limits, enhanced speciation insights, and powerful data analytics and modeling tools, modern approaches support groundwater monitoring and pollution source tracking. Nevertheless, challenges such as matrix interferences, instability of metal species during sampling, and overlapping source signatures continue to complicate analysis, underscoring the need for integrated approaches that merge analytical innovation with data-driven tools to refine source attribution and ultimately ensure groundwater safety. Critical knowledge gaps are identified, including the need for more consistent monitoring approaches, closer integration of new analytical tools with field practice, and stronger policies to make sure scientific progress actually leads to better groundwater protection.

地下水重金属污染是一个普遍存在的全球性问题，威胁着饮用水质量和公众健康。有效的缓解需要稳健的分析方法和预测建模框架。这篇综述批判性地评估了用于检测和理解地下水中HM污染的尖端仪器技术和来源分配方法。以电感耦合等离子体质谱法（ICP-MS）为例的先进实验室技术现在实现了非凡的灵敏度，检测限在亚μg/L范围内，并具有很高的精度（相对标准偏差在2 - 3%之间）。此外，联用色谱光谱技术，如离子色谱与ICP-MS耦合，有助于详细的化学形态形成，有效地分辨环境浓度（1-50 μ g/L）下的氧化还原敏感对，如Cr(III)/Cr（VI）和as (III)/ as (V)。同时，新兴的电化学便携式传感器使现场实时监测地下水质量成为可能。对于来源分配，多元统计方法（包括受体模型）、同位素指纹和机器学习算法已经越来越多地集成在一起，以解开自然与人为金属来源的复杂混合物。通过结合改进的检测极限、增强的物种形成洞察力以及强大的数据分析和建模工具，现代方法支持地下水监测和污染源跟踪。然而，诸如基质干扰、采样过程中金属种类的不稳定性以及源特征重叠等挑战继续使分析复杂化，强调了将分析创新与数据驱动工具相结合的综合方法的必要性，以改进源属性，最终确保地下水安全。报告指出了关键的知识差距，包括需要更一致的监测方法、更紧密地将新的分析工具与实地实践结合起来，以及更强有力的政策，以确保科学进步实际上带来更好的地下水保护。

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引用次数: 0

Strategies for resin reuse in Sr/matrix separation prior to isotope analysis 同位素分析前Sr/基质分离中树脂再利用策略

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-02 DOI: 10.1016/j.talo.2025.100571

Agnese Aguzzoni, Giulio Voto, Werner Tirler

One of the key passages to achieve an accurate and precise Sr isotope ratio (⁸⁷Sr/⁸⁶Sr) measurement is the Sr/matrix separation. Traditionally this step is accomplished using a Sr-spec resin which allows the Sr recovery eliminating the main interferent species (namely Ca, Rb, Pb). While this application is consolidated, few details are available in literature regarding the possibility to reuse the resin for the consecutive separation of Sr from multiple samples. To deepen this aspect, in this study different tests were conducted both using certified and in-house reference materials, to check the performance of the separation in different working conditions and testing solutions of HNO₃ and HCl for the resin washing between consecutive use cycles. These tests confirmed that resin reuse guarantees average Sr recovery higher than 95 %, with a negligible presence of interfering species in the final solution, and no effect on the Sr isotope ratios. However, since in no case a quantitative Sr recovery was achieved, the presence of Sr residues on the resin might limit this application if different samples are treated on the same resin. Tests with batches of real samples showed that using a mild washing with HNO₃ (0.05 M), although residual cross-contamination was present, no significant alteration of the sample ⁸⁷Sr/⁸⁶Sr ratios occurred, in case of homogeneous ratios. At increasing levels of heterogeneity, even the use of HCl in the resin washing might be not sufficient. This study demonstrated the feasibility of resin reuse, reducing its consumption, associated costs, and lab workload, without affecting the accuracy of the results when working with sample replicates or when a low-to-medium difference in the ⁸⁷Sr/⁸⁶Sr ratio of consecutive samples is expected.

实现Sr同位素比值（87Sr/86Sr）精确测量的关键环节之一是Sr/基质分离。传统上，这一步是使用Sr-spec树脂完成的，该树脂允许Sr回收消除主要干扰物质（即Ca， Rb, Pb）。虽然这种应用得到了巩固，但文献中很少有关于从多个样品中连续分离Sr的树脂的可能性的细节。为了深化这方面的研究，在本研究中，使用认证和内部标准物质进行了不同的测试，以检查不同工况下的分离性能，并在连续使用周期之间测试HNO3和HCl溶液用于树脂洗涤。这些测试证实，树脂重复使用保证Sr的平均回收率高于95%，最终溶液中干扰物质的存在可以忽略不计，并且对Sr同位素比率没有影响。然而，由于在任何情况下都没有实现定量的Sr回收，如果在同一树脂上处理不同的样品，树脂上Sr残留物的存在可能会限制这种应用。对一批实际样品进行的测试表明，使用HNO3 （0.05 M）温和洗涤，尽管存在残留的交叉污染，但在均匀比例的情况下，样品87Sr/86Sr比率没有发生显着变化。在非均质性增加的情况下，即使在树脂洗涤中使用盐酸也可能是不够的。本研究证明了树脂重复使用的可行性，减少了树脂的消耗、相关成本和实验室工作量，并且在处理样品重复或预期连续样品的87Sr/86Sr比例存在中低差异时，不会影响结果的准确性。

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引用次数: 0

Quantitative results of tocopherols, sterols, triterpene esters, and lipid degradation products in shea kernels using native or quantifier ion intensity corrected analogue standards 定量结果生育酚，甾醇，三萜酯，脂质降解产物在乳木果仁使用原生或定量离子强度校正类似物标准

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-30 DOI: 10.1016/j.talo.2025.100570

Kit Granby , Paula Toma , Peter Christensen , Ella Walbeogo , Yaya Dao , Tore K Ravn , Jan H Christensen , Nikoline J Nielsen

In shea kernels from the Vitellaria Paradoxa tree, lipids make up approximately half of the mass. The lipid portion includes a significant proportion of non-glyceridic lipids, also known as unsaponifiable matter (USM). Shea kernel samples from a controlled study in Burkina Faso, investigating local storage conditions in covered soil pits, were analyzed for USM compounds using GC–MS. The quantification was performed using external calibration curves of native (exact compound) or analogue (chemically similar) standards, and relative intensity corrections of quantifier ions were based on EI mass spectra of standards, samples, and MS libraries. The USM compounds amounted to 2.9 ± 0.4 g/100 g (n = 19) of the shea kernel samples. They included three tocopherols, two Δ⁷-sterols, squalene, and triterpenes, where the triterpenes accounted for an average of 98 % of the total quantified beneficial compounds. The triterpenes included four triterpene alcohols, comprising 1 %, ten triterpene acetates, and eight triterpene cinnamates, comprising 97 % of the quantified unsaponifiable extractables. The concentration of the four predominant tetra- and pentacyclic triterpene cinnamate esters were: α-amyrin 907 ± 110 mg/100 g kernels, lupeol 577 ± 73 mg/100 g, butyrospermol 264 ± 44 mg/100 g, and β-amyrin 130 ± 17 mg/100 g. The triterpene acetates were less abundant; the four dominant were of the triterpenes: α-amyrin 268 ± 38 mg/100 g, lupeol 203 ± 39 mg/100 g, butyrospermol 165 ± 32 mg/100 g, and β-amyrin 68 ± 9 mg/100 g. The quantified USM compounds are known to possess antioxidative, anti-inflammatory, and related health effects. Of particular interest are the tetracyclic and pentacyclic triterpene esters due to their exceptionally high levels in shea butter compared to other oils and fats.

在牛油果核从Vitellaria Paradoxa树，脂质约占一半的质量。脂质部分包括非甘油脂质的显著比例，也被称为不可皂化物质（USM）。在布基纳法索的一项对照研究中，研究了当地覆盖土壤坑的储存条件，使用气相色谱-质谱分析了USM化合物。采用天然（精确化合物）或类似物（化学相似）标准品的外部校准曲线进行定量，定量剂离子的相对强度校正基于标准品、样品和质谱库的EI质谱。在乳木果仁样品中，USM化合物含量为2.9±0.4 g/100 g （n = 19）。它们包括三种生育酚，两种Δ7-sterols，角鲨烯和三萜，其中三萜平均占总量化有益化合物的98%。三萜包括四种三萜醇，含1%，十种三萜乙酸酯和八种三萜肉桂酸酯，含97%的定量不可皂化萃取物。肉桂酸四、五环三萜酯的含量分别为：α-amyrin 907±110 mg/ 100g， luppeol 577±73 mg/ 100g, butyrospermol 264±44 mg/ 100g, β-amyrin 130±17 mg/ 100g。乙酸三萜含量较少；结果表明：α-amyrin（268±38 mg/100 g）、luppeol（203±39 mg/100 g）、butyrosspermol（165±32 mg/100 g）、β-amyrin（68±9 mg/100 g）占主导地位。已知定量USM化合物具有抗氧化，抗炎和相关的健康作用。特别令人感兴趣的是四环和五环三萜酯，因为与其他油和脂肪相比，乳木果油中的含量异常高。

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引用次数: 0

Sensitive detection Cu/Zn SOD mRNA with CRISPR/Cas9 利用CRISPR/Cas9灵敏检测Cu/Zn SOD mRNA

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-29 DOI: 10.1016/j.talo.2025.100568

Geng Qiuyu , Xing Hanxin , Cang Song , Fan Ronghua , Zheng Anqi

Cu/Zn SOD mRNA plays a critical role in protecting cells from oxygen toxicity by regulating the expression of the Cu/Zn SOD enzyme. In this work, we developed a biosensor, termed the Cas9-sgRNA/blocker system, for the analysis of Cu/Zn SOD mRNA. The biosensor was assembled by hybridizing a crRNA with a complementary blocker strand, which was then complexed with the Cas9 nuclease. In the presence of the target mRNA, competitive binding between the blocker strand and the mRNA restored Cas9-sgRNA cleavage activity, enabling enzymatic cleavage of adjacent fluorescent dsDNA reporters. Fluorescence measurements were performed at λex/λem = 488/525 nm, yielding a detection limit of 1.40 nmol L⁻¹. This method demonstrated excellent selectivity for Cu/Zn SOD mRNA, as evidenced by its successful application to the detection of Cu/Zn SOD mRNA in real samples.

Cu/Zn SOD mRNA通过调节Cu/Zn SOD酶的表达，在保护细胞免受氧毒性中起着关键作用。在这项工作中，我们开发了一种生物传感器，称为Cas9-sgRNA/阻断剂系统，用于分析Cu/Zn SOD mRNA。通过将crRNA与互补的阻滞剂链杂交组装生物传感器，然后将其与Cas9核酸酶络合。在目标mRNA存在的情况下，阻断链和mRNA之间的竞争性结合恢复了Cas9-sgRNA的切割活性，使相邻的荧光dsDNA报告基因能够被酶切。荧光测量在λex/λem = 488/525 nm处进行，检测限为1.40 nmol L⁻¹。该方法对Cu/Zn SOD mRNA具有良好的选择性，并成功应用于实际样品中Cu/Zn SOD mRNA的检测。

引用次数: 0

Addition of apricot powder and its extracts on the physicochemical properties and sensory attributes of Reconstituted Tobacco 杏粉及其提取物的添加对再造烟草理化特性和感官特性的影响

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-28 DOI: 10.1016/j.talo.2025.100567

Gaofeng Dong , Weiyao Hu , Jing Liu , Guiyuan Zhou , Kai Li , Jin Xu , Olivier Berthou , Huifan Li , Yuxin Liu

Reconstituted tobacco (RT), is a product for reducing tar and harm in cigarettes. It is also the main means of resource utilization of tobacco waste. However, RT products are often limited in their wide application due to the traditional monotonic flavor from the poor stability of added flavor agents during processing. It is of considerable interest to investigate flavor diversification of RT. In this study, the effects of ultra-fine apricot powder with different particle sizes and addition rates on the characteristics of RT were investigated. The result shows to optimize the performance of physical and chemical indexes of RT, an 800 mesh is the most appropriate particle size and the powder addition rate should be below 15 %. The uniformity of dry weight improved 26.0 %, color difference (L*, a*, b*) 29.1 %, 23.5, 33.8 % respectively. The color difference (ΔE) between it and the standard sample of natural tobacco leaves optimized by 38.7 %. 14 % apricot powder added in forming pulp and 0.15 % apricot extracts added in coating liquid is the best combination in obtaining RT finished products with apricot characteristic aroma and natural fruity taste. These results reveal a potential method for developing high-quality natural plant flavored RT.

再造烟草（RT）是一种降低卷烟中焦油含量和危害的产品。也是烟草废弃物资源化利用的主要手段。然而，由于在加工过程中添加的风味剂稳定性差，传统的风味单调，制约了RT产品的广泛应用。研究杏粉的风味多样性是研究杏粉风味多样性的重要课题。本研究研究了不同粒径和添加量的超细杏粉对杏粉风味的影响。结果表明，为优化RT的理化指标性能，800目为最佳粒度，粉料添加量应低于15%。干重均匀度提高26.0%，色差（L*、a*、b*）分别提高29.1%、23.5%、33.8%。与天然烟叶标准样品的色差（ΔE）优化为38.7%。成浆中添加14%杏粉，包衣液中添加0.15%杏提取物，可获得具有杏特有香气和天然果味的RT成品。这些结果揭示了一种开发高品质天然植物风味RT的潜在方法。

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引用次数: 0

Analysis of antioxidant properties of pomegranate (Punica granatum L.) Peel extract: Voltammetric, spectrophotometric, and in silico studies as a potential antidiabetic candidate 石榴的抗氧化性能分析果皮提取物：伏安法，分光光度法和硅片研究作为潜在的抗糖尿病候选者

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-28 DOI: 10.1016/j.talo.2025.100569

Ari Yuniarto , Junaidin , Abdul Aziz Setiawan , Dadang Juanda , Ardi Ardiansyah , Vienna Saraswaty

Pomegranate (Punica granatum L.) peel, a common byproduct of the agroindustry, contains important antioxidant compounds. This study investigates the antioxidant and antidiabetic properties of pomegranate peel water extract (PPE) using both in vitro and in silico approaches. We evaluated the antioxidant activity using the voltammetric method and DPPH assays, supported by the determination of total phenolic content (TPC) and total flavonoid content (TFC). The direct antioxidant voltammetric evaluation revealed the presence of strong antioxidant compounds with an electrochemical index of 1.12 µA/mV. When the PPE was tested indirectly using K₃Fe(CN)₆ solution, the electrochemical index was increased up to 168.0 µA/mV, indicating a synergistic effect of both electroactive and non-electroactive antioxidant compounds. The DPPH assays also support the presence of strong antioxidant compounds with an IC₅₀ of 17 µg/mL. TPC and TFC values were 8.14 ± 0.373 µg GAE/g dry extract and 1.69 ± 0.003 µg QE/g dry extract, respectively. The antidiabetic potency was analyzed via α-glucosidase inhibition assay, yielding an IC₅₀ of 176.73 µg/mL for PPE. The molecular docking demonstrated that punicalagin, a major bioactive compound present in PPE, exhibited strong binding affinity to α-glucosidase enzyme (−10.42 kcal/mol), surpassing that of acarbose (−10.16 kcal/mol). These findings suggest that PPE is a promising natural source of antioxidant and antidiabetic agents, with potential applications in functional food or therapeutic formulations.

石榴皮是一种常见的农业副产品，含有重要的抗氧化化合物。研究了石榴皮水提取物（PPE）的抗氧化和抗糖尿病作用。采用伏安法和DPPH法测定其抗氧化活性，并测定其总酚含量（TPC）和总黄酮含量（TFC）。直接抗氧化伏安评价表明，存在强抗氧化化合物，电化学指数为1.12µA/mV。在K3Fe(CN)6溶液中间接测试PPE时，电化学指标提高到168.0µA/mV，表明电活性和非电活性抗氧化化合物具有协同作用。DPPH分析也支持强抗氧化化合物的存在，IC50为17µg/mL。TPC值为8.14±0.373µg GAE/g干提取物，TFC值为1.69±0.003µg QE/g干提取物。通过α-葡萄糖苷酶抑制实验分析其抗糖尿病功效，得到PPE的IC₅₀为176.73µg/mL。分子对接结果表明，槟榔苷对α-葡萄糖苷酶具有较强的结合亲和力（- 10.42 kcal/mol），优于阿卡波糖（- 10.16 kcal/mol）。这些发现表明，PPE是一种很有前景的抗氧化和抗糖尿病药物的天然来源，在功能性食品或治疗制剂中具有潜在的应用前景。

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引用次数: 0

The versatility of metal-organic frameworks-based biosensor for antioxidant detection 基于金属-有机框架的抗氧化剂检测生物传感器的多功能性

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-26 DOI: 10.1016/j.talo.2025.100566

Siti Unvaresi Misonia Beladona , Rizki Rachmad Saputra , Aep Patah , Meiyanti Ratna Kumalasari

Antioxidants are crucial in mitigating oxidative stress caused by reactive oxygen and nitrogen species (ROS/RNS), contributing to chronic diseases and biomolecular damage. Beyond their biological significance, antioxidants are widely used in food, cosmetics, and pharmaceuticals to enhance stability and shelf life. Conventional detection methods—such as spectrophotometric assays (DPPH, ABTS, ORAC) and chromatographic techniques (HPLC, LC-MS)—are accurate but suffer from high costs, complex workflows, and limited portability. Biosensors offer a promising alternative, combining high sensitivity, rapid analysis, and real-time monitoring. Among these, metal-organic framework (MOFs) based biosensors stand out due to their tunable porosity, high surface area, and multifunctional design, enabling precise antioxidant detection via fluorescent, colorimetric, electrochemical, or hybrid mechanisms. These sensors achieve enhanced selectivity and signal amplification by integrating MOFs with various functional materials (e.g., graphene, gold nanoparticles (AuNPs)) or biomimetic catalysts (nanozymes), even in complex matrices like biological fluids or food extracts. However, challenges remain in improving commercial applications’ selectivity, stability, and scalability. This review examines MOF-based biosensing platforms, their design strategies, and detection mechanisms while addressing key obstacles in transitioning from lab-scale to real-world deployment. By highlighting recent advances and unmet needs, we aim to guide the development of next-generation biosensors for antioxidant monitoring in clinical, industrial, and environmental settings.

抗氧化剂在减轻由活性氧和氮物种（ROS/RNS）引起的氧化应激中起着至关重要的作用，有助于慢性疾病和生物分子损伤。除了其生物学意义外，抗氧化剂还广泛用于食品、化妆品和药品中，以提高稳定性和保质期。传统的检测方法，如分光光度法（DPPH， ABTS， ORAC）和色谱技术（HPLC, LC-MS），都是准确的，但成本高，工作流程复杂，便携性有限。生物传感器提供了一种很有前途的替代方案，结合了高灵敏度、快速分析和实时监测。其中，基于金属有机框架（mof）的生物传感器因其可调孔隙率、高表面积和多功能设计而脱颖而出，能够通过荧光、比色、电化学或混合机制进行精确的抗氧化剂检测。这些传感器通过将mof与各种功能材料（如石墨烯、金纳米颗粒（AuNPs））或仿生催化剂（纳米酶）集成，甚至在生物流体或食物提取物等复杂基质中，也能实现增强的选择性和信号放大。然而，在提高商业应用程序的选择性、稳定性和可伸缩性方面仍然存在挑战。本文综述了基于mof的生物传感平台，它们的设计策略和检测机制，同时解决了从实验室规模过渡到现实世界部署的关键障碍。通过强调最近的进展和未满足的需求，我们的目标是指导下一代生物传感器的发展，用于临床，工业和环境环境中的抗氧化剂监测。

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引用次数: 0

A straightforward HPLC approach to testing butylated hydroxytoluene, an antioxidant, in pure and topical anti-burn gels; Evaluation of greenness, blueness, and whiteness grades 一种直接的高效液相色谱方法测试纯和局部抗烧伤凝胶中的抗氧化剂丁基羟基甲苯；评价绿色、蓝色和白色等级

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-23 DOI: 10.1016/j.talo.2025.100565

Jin-Kui Ma , Xiao-Yang Chen , Nan Zhang , Ahmed Sobhy Darwish , Ayman A. Gouda , Ragaa El Sheikh , Xiao-Chen Huang

In lieu of being used in anti-burn gels for medicinal reasons, butylated hydroxytoluene is used as a stabilizer and antioxidant to prolong the gel's lifetime and safeguard its effectiveness by halting the oxidation and degradation of other ingredients, especially fats and oils. It shields the product's texture, color, and smell against alterations brought on by light and air exposure. Antioxidants can be used to guard the product quality during the manufacturing process only and may not need to be controlled during its shelf life. A comprehensive, rapid, clear, and environmentally friendly stability-indicating liquid chromatography (LC) technique has been developed and validated, enabling the quantification of butylated hydroxytoluene levels in topical gels. The pharmaceutical compound underwent a series of evaluations in line with ICH standards. Through isocratic separation, butylated hydroxytoluene and its associated degradants were successfully distinguished. The antioxidant was quantified and validated utilizing the Kromasil C-18 stationary phase at a temperature of 45° C. The isocratic mobile phase functions at a flow rate of 2.0 mL/min. and comprises fixed proportions of filtered water, acetonitrile, and methanol. The overall AES grade of 77, AGREE grade of 0.6, AGREEprep grade of 0.6, CACI grade of 65, AGSA grade of 63.89, MoGAPI grade of 70, BAGI grade of 77.5, and ultimate whiteness of 96.3 collectively underscore the environmental benefits of the LC method. Research suggests that the proposed approach is distinctive, accurate, reliable, robust, and ecologically advantageous, rendering it appropriate for routine quality control assessments.

代替用于抗烧伤凝胶的药用原因，丁基羟基甲苯被用作稳定剂和抗氧化剂，以延长凝胶的使用寿命，并通过阻止其他成分，特别是脂肪和油的氧化和降解来保障其有效性。它可以保护产品的质地、颜色和气味不受光线和空气暴露的影响。抗氧化剂仅在生产过程中用于保护产品质量，在保质期内可能不需要控制。建立并验证了一种全面、快速、清晰、环保的稳定性指示液相色谱（LC）技术，可以定量测定外用凝胶中丁基羟基甲苯的含量。该药物化合物经过了一系列符合ICH标准的评估。通过等密度分离，成功地分离出了丁基羟基甲苯及其伴生降解物。采用Kromasil C-18固定相，在45℃的温度下对抗氧化剂进行定量和验证，等压流动相的流速为2.0 mL/min。由固定比例的过滤水、乙腈和甲醇组成。AES总等级为77，AGREE等级为0.6，AGREEprep等级为0.6，CACI等级为65，AGSA等级为63.89，MoGAPI等级为70，BAGI等级为77.5，最终白度为96.3，共同强调了LC法的环境效益。研究表明，该方法具有独特、准确、可靠、稳健和生态优势，适用于常规质量控制评估。

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