Talanta Open最新文献_第6页

Plasmonic fingerprinting: next-generation SERS architectures for sensitive heavy metal quantification 等离子体指纹识别：用于敏感重金属量化的下一代SERS架构

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-09 DOI: 10.1016/j.talo.2025.100554

Muhammad Sarkawi , Rupam Sharma , Geetha Venkatesan , Tripti Ahuja , Soumyanti Panda , Jai Prakash , Hideki Kuramitz , K.S. Shalini devi

The global heavy metal crisis demands sensing technologies that transcend traditional analytical limitations, positioning Surface-Enhanced Raman Scattering (SERS) as the transformative solution for real-world environmental and health monitoring challenges. This comprehensive review illuminates the sophisticated engineering principles underlying modern SERS architectures, where nanoscale precision meets molecular recognition to achieve detection limits that challenge the boundaries of single-molecule spectroscopy. Additionally, a systematic analysis of various SERS sensing strategies, including label-free approaches utilizing direct metal-nanoparticle interactions, molecular probe-based systems employing Raman-active chelating agents, and advanced functionalized nanoparticle platforms incorporating DNA aptamers, peptides, and polymeric recognition elements are discussed. Representative case studies include the successful detection of mercury in contaminated fish samples at sub-regulatory levels, lead quantification in urban drinking water systems, and arsenic monitoring in rice cultivation areas, showcasing the versatility and reliability of SERS-based approaches across diverse analytical challenges. The review concludes with a progressive perspective on the merging of SERS technology with microfluidics, Internet of Things architectures, and distributed sensing networks that will replace the current approach of testing samples periodically with continuous monitoring of heavy metals at specific locations.

全球重金属危机需要超越传统分析限制的传感技术，将表面增强拉曼散射（SERS）定位为现实世界环境和健康监测挑战的变革性解决方案。这篇全面的综述阐明了现代SERS架构背后的复杂工程原理，其中纳米级精度满足分子识别，以实现挑战单分子光谱边界的检测极限。此外，本文还讨论了各种SERS传感策略的系统分析，包括利用直接金属-纳米颗粒相互作用的无标记方法，利用拉曼活性螯合剂的基于分子探针的系统，以及结合DNA适体、肽和聚合物识别元件的高级功能化纳米颗粒平台。代表性案例研究包括成功检测亚监管水平受污染鱼类样本中的汞，城市饮用水系统中的铅量化以及水稻种植区域的砷监测，展示了基于sers的方法在各种分析挑战中的多功能性和可靠性。该综述总结了SERS技术与微流体、物联网架构和分布式传感网络相结合的进步观点，这些技术将取代目前定期检测样品并在特定位置连续监测重金属的方法。

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引用次数: 0

Fc-tagged PLA2R antigen enables high-performance chemiluminescent detection for non-invasive idiopathic membranous nephropathy diagnosis fc标记的PLA2R抗原可用于非侵袭性特发性膜性肾病的高效化学发光检测

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-09 DOI: 10.1016/j.talo.2025.100555

Jiangjian Liu , Qiong Liang , Yanhang Zhuo, Sunhui Chen

Idiopathic membranous nephropathy (IMN) is characterized by anti-phospholipase A2 receptor (PLA2R) antibodies, yet current detection methods lack sensitivity or are invasive. We developed a chemiluminescent assay to address these limitations. Three PLA2R antigens (Fc-tagged full, His-tagged, D3 core segment) were expressed in Expi293E cells and purified. Goat/mouse anti-human IgG antibodies were compared for detection efficiency. Kit stability was evaluated at 4 °C and 37 °C. Clinical validation included 75 biopsy-confirmed cases (50 IMN, 25 non-IMN), comparing the kit’s performance against a commercial ELISA. The PLA2R-Fc antigen demonstrated 100 % sensitivity and specificity, outperforming His-tagged (100 % sensitivity, 97 % specificity) and D3-truncated variants (60 % sensitivity). Coating in pH 6.0 MES buffer achieved the highest signal-to-noise ratio (45.08 vs. 25.76 in HEPES). Goat anti-human IgG enabled lower detection limits (22.5 U mL⁻¹ vs. 45 U mL⁻¹ for monoclonal antibody). The kit retained stable over 12 months at 4 °C. In clinical testing, the chemiluminescent assay showed superior concordance with histopathology, higher sensitivity, and specificity, particularly for low-titer samples. This chemiluminescent anti-PLA2R antibody assay combines high sensitivity, specificity, and stability, enabling non-invasive IMN diagnosis and therapeutic monitoring. Its technical optimizations address key limitations of existing methods, offering a reliable diagnostic tool for IMN.

特发性膜性肾病（IMN）以抗磷脂酶A2受体（PLA2R）抗体为特征，但目前的检测方法缺乏敏感性或具有侵入性。我们开发了一种化学发光试验来解决这些局限性。三种PLA2R抗原（fc标记full， his标记，D3核心段）在Expi293E细胞中表达并纯化。比较山羊/小鼠抗人IgG抗体的检测效率。在4°C和37°C时评估试剂盒的稳定性。临床验证包括75例活检确诊病例（50例IMN， 25例非IMN），将试剂盒的性能与商业ELISA进行比较。PLA2R-Fc抗原表现出100%的敏感性和特异性，优于his标记的（100%敏感性，97%特异性）和d3截断的变体（60%敏感性）。在pH 6.0的MES缓冲液中涂层获得了最高的信噪比（45.08 vs. 25.76 HEPES）。山羊抗人IgG的检出限较低（单克隆抗体为22.5 U mL−1，单克隆抗体为45 U mL−1）。试剂盒在4°C下保持稳定超过12个月。在临床测试中，化学发光法显示出与组织病理学的优越一致性，更高的灵敏度和特异性，特别是对低滴度样品。这种化学发光抗pla2r抗体检测结合了高灵敏度、特异性和稳定性，使非侵入性IMN诊断和治疗监测成为可能。其技术优化解决了现有方法的主要局限性，为IMN提供了可靠的诊断工具。

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引用次数: 0

Validation of combination of protodioscin and shatavarin iv from medicinal extracts for alleviating menopausal symptoms by computational deep learning models 通过计算深度学习模型验证药用提取物中原薯蓣皂苷和沙伐林iv联合用于缓解更年期症状的有效性

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-08 DOI: 10.1016/j.talo.2025.100552

Rithika Naveencharan , Ashwin Sivakumar , Rishi Senthil Kumar , Sheena Christabel Pravin , Reena Monica P , V. Kiruthika , Abhijeet Morde , Arun Balakrishnan , Muralidhara Padigaru , Ninad Puranik , Ravindra Nayakwadi , Tejas Namjoshi

Shatavarin-IV and Protodioscin, saponins of Asparagus racemosus and Trigonella foenum-graecum respectively are phytoestrogens commonly used to alleviate post-menopausal symptoms such as hot flashes, sleeping disorder, and mood sways. While their individual benefits are well-documented, the synergistic effects of these compounds remain underexplored. This study aims to validate the combination of Shatavarin-IV and Protodioscin on molecular mediators of menopausal symptoms using advanced computational analyses. Deep learning model for drug additivity analysis was developed and trained on the DrugComb database using the Loewe’s additivity score as the target variable, with a focus on combinations tested on the MCF7 cell line and validated of the 786-O cell line. Molecular docking simulations were performed using PyRx, PyMOL, FPocket and ChimeraX to investigate the binding affinities of Protodioscin and Shatavarin-IV with key neuronal modulators of hot flashes, including Kisspeptin, Neurokinin B, TRPV1, c-FOS, and GnRH. IC50 values were derived from binding free energies. Dose-response simulations were evaluated across concentrations (0.01 µM to 100 µM) using a four-parameter logistic equation. Absorption, Distribution, Metabolism, and Elimination (ADME) profiling was determined to predict bioavailability and toxicity. The dose-response simulations confirmed significant therapeutic potential at relevant concentrations and additive interaction between both the compounds suggests enhanced therapeutic efficacy. ADME profiling indicated favorable bioavailability, non-toxicity, and efficient metabolism for both compounds and provides novel insights into their molecular mechanism of action in the body. The results of this research indicate that the combined additive effect of Protodioscin and Shatavarin-IV in alleviating menopausal symptoms exhibits favorable pharmacokinetic profile, and could potentially be promising candidates for future human clinical studies.

总状芦笋皂苷和三角芦笋皂苷原薯蓣皂苷分别是植物雌激素，常用于缓解绝经后潮热、睡眠障碍、情绪波动等症状。虽然它们各自的益处已被充分证明，但这些化合物的协同效应仍未得到充分探讨。本研究旨在通过先进的计算分析验证Shatavarin-IV和原薯蓣皂苷对绝经期症状分子介质的联合作用。以Loewe加性评分为目标变量，在DrugComb数据库上开发并训练了药物加性分析的深度学习模型，重点对MCF7细胞系和786-O细胞系进行了组合测试和验证。利用PyRx、PyMOL、FPocket和ChimeraX进行分子对接模拟，研究Protodioscin和Shatavarin-IV与热潮热的关键神经元调节剂Kisspeptin、Neurokinin B、TRPV1、c-FOS和GnRH的结合亲和力。IC50值由束缚自由能导出。使用四参数logistic方程评估不同浓度（0.01µM至100µM）的剂量-反应模拟。吸收、分布、代谢和消除（ADME）分析被确定预测生物利用度和毒性。剂量-反应模拟证实了在相关浓度下显著的治疗潜力，两种化合物之间的加性相互作用表明治疗效果增强。ADME分析表明，这两种化合物具有良好的生物利用度、无毒性和有效的代谢，并为其在体内的分子作用机制提供了新的见解。本研究结果表明，原diooscin和Shatavarin-IV在缓解绝经期症状方面的联合加性作用表现出良好的药代动力学特征，可能是未来人类临床研究的潜在有希望的候选者。

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引用次数: 0

Thorium determination in seawater by inductively coupled plasma mass spectrometry: Method development and oceanographic applications 电感耦合等离子体质谱法测定海水中的钍：方法发展和海洋学应用

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-08 DOI: 10.1016/j.talo.2025.100553

Yingjie Zou , Peng Wang , Lei Xu , Hong Ren , Yiyao Cao

Thorium isotopes in the ocean (including radiogenic isotopes and terrigenous-derived ²³²Th) serve as key tracers for studying land-derived material transport. While high-sensitivity and high-resolution inductively coupled plasma mass spectrometry (ICP-MS) has become the mainstream technique for seawater thorium isotope analysis, its application faces three major challenges: complex pretreatment purification procedures, varying sample volume requirements, and significant instrument memory effects. This review systematically summarizes the technical difficulties and solutions in ICP-MS-based thorium isotope detection and examines its oceanographic applications in quantifying dust inputs and tracing water mass mixing, thereby providing critical data for assessing marine carbon pump efficiency.

海洋中的钍同位素（包括放射性同位素和陆源源的232Th）是研究陆源物质运输的关键示踪剂。虽然高灵敏度、高分辨率电感耦合等离子体质谱（ICP-MS）已成为海水钍同位素分析的主流技术，但其应用面临三大挑战：复杂的预处理纯化程序、不同的样品体积要求和显著的仪器记忆效应。本文系统总结了基于icp - ms的钍同位素检测的技术难点和解决方案，并探讨了其在定量粉尘输入和跟踪水团混合方面的海洋应用，从而为评估海洋碳泵效率提供关键数据。

引用次数: 0

Renewable carbon from flax shives with silver nanoparticles biosynthesized using Eichhornia crassipes extract for green electrochemical detection of hydroxychloroquine 以亚麻籽提取物为原料合成可再生碳纳米银用于绿色电化学检测羟氯喹

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-05 DOI: 10.1016/j.talo.2025.100548

Francisco Contini Barreto , Gloria Tersis Vieira dos Santos , Maria Eduarda Barberis , Naelle Kita Mounienguet , Martin Kássio Leme da Silva , Quan He , Ivana Cesarino

Hydroxychloroquine (HCQ) is an antimalarial drug that was repurposed during the COVID-19 pandemic. However, due to its limited clinical efficacy and notable side effects, its recommendation was later withdrawn. Despite this, HCQ sales increased by over 800 % in Brazil, and its presence has since been detected in aquatic environments, raising concerns regarding potential risks to both environmental and human health. In response, a novel green electrochemical sensor was developed for the determination of HCQ in water samples. The sensor is based on a glassy carbon electrode modified with hydrochar derived from flax shives and silver nanoparticles biosynthesized using Eichhornia crassipes (water hyacinth) extract as a reducing and stabilizing agent (GC/HC-AgNPs). The sensor was characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and cyclic voltammetry (CV). Experimental parameters were optimized, and the device was successfully applied for the detection of HCQ using differential pulse voltammetry (DPV). The sensor exhibited a linear response in the range of 1.0–40.0 µmol L⁻¹, with a limit of detection (LOD) of 0.265 µmol L⁻¹ and a limit of quantification (LOQ) of 0.885 µmol L⁻¹. Excellent repeatability (RSD = 2.27 %) and reproducibility (RSD = 5.75 %) were achieved. Application in tap and lake water samples resulted in recovery values ranging from 89.5 % to 105.0 %, and comparative analysis with UV–vis spectroscopy confirmed the accuracy of the proposed method. The sensor further demonstrated good selectivity even in the presence of relevant interfering species. Green chemistry assessment tools confirmed the sustainable character of the developed method. Overall, the proposed sensor represents a promising, effective, and environmentally friendly platform for HCQ monitoring in water matrices.

羟氯喹（HCQ）是一种抗疟疾药物，在COVID-19大流行期间被重新利用。然而，由于其临床疗效有限，副作用明显，后来撤销了推荐。尽管如此，HCQ在巴西的销量增长了800%以上，此后在水生环境中发现了HCQ的存在，引发了人们对环境和人类健康潜在风险的担忧。为此，研制了一种新型绿色电化学传感器，用于水样中HCQ的测定。该传感器基于一种玻璃碳电极，该电极由亚麻屑中提取的碳氢化合物修饰，而银纳米粒子是用水葫芦提取物作为还原剂和稳定剂（GC/HC-AgNPs）生物合成的。采用扫描电子显微镜（SEM）、能量色散x射线能谱（EDS）和循环伏安法（CV）对传感器进行了表征。对实验参数进行了优化，成功地将该装置应用于差分脉冲伏安法（DPV）检测HCQ。该传感器在1.0-40.0µmol L⁻¹范围内呈线性反应，检测限为0.265µmol L⁻¹，定量限为0.885µmol L⁻¹。重复性好（RSD = 2.27%），重现性好（RSD = 5.75%）。应用于自来水和湖泊水样，回收率为89.5% ~ 105.0%，与紫外-可见光谱对比分析证实了该方法的准确性。该传感器在相关干扰物质存在的情况下也表现出良好的选择性。绿色化学评价工具证实了所开发方法的可持续性。总的来说，所提出的传感器代表了一个有前途的、有效的、环保的平台，用于监测水基质中的HCQ。

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引用次数: 0

Electrochemical detection of enrofloxacin and ciprofloxacin based on mesoporous La2O2CO3 nanostructures – an eco-friendly electrode material for sustainability 基于介孔La2O2CO3纳米结构的可持续性环保电极材料电化学检测恩诺沙星和环丙沙星

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-03 DOI: 10.1016/j.talo.2025.100546

Arghya Chakravorty , Sudip Das , Sahil Luktuke , Aarcha Appu Mini , Vimala Raghavan

Administration of antibiotics in the modern world has posed a serious threat to induce antimicrobial resistance (AMR), which concerns the Sustainable Development Goals (SDGs). Fluoroquinolone group antibiotics, namely enrofloxacin and ciprofloxacin, are predominantly responsible for undermining the One Health. In this context, to provide an eco-friendly and robust approach, a green electrochemical sensor has been fabricated with lanthanum oxycarbonate (La₂O₂CO₃) nanoparticles for the detection of enrofloxacin and ciprofloxacin in several complex matrices, related to food safety, environmental monitoring, and human health. The green synthesis methodology was adopted for the synthesis of the oxycarbonate material, while the phytochemicals present in the leaves of Sida acuta played the role of reducing and capping agents. To characterize the crystallinity and morphology of the synthesized material, several spectroscopic (XRD, BET, EDAX) and microscopic (FESEM, TEM) techniques were employed. The fabricated electrodes were characterized by atomic force microscopy (AFM), goniometry, and surface profilometry. The fabricated voltammetric sensor demonstrated an efficient linear range of 2.77 µM to 158.99 µM and 5.98 µM to 57.87 µM in the case of enrofloxacin and ciprofloxacin, respectively. In both cases, cyclic voltammetry (CV), differential pulse voltammetry (DPV), and square wave voltammetry (SWV) have been employed as electrochemical analytical techniques. The limit of detection (LOD) was achieved to be 4.07 µM, 2.91 µM, and 4.98 µM, while the limit of quantification (LOQ) was achieved to be 14.2 µM, 8.83 µM, and 15.1 µM in CV, DPV, and SWV, respectively, for the detection of enrofloxacin. On the other hand, the LOD was achieved to be 2.85 µM, 1.90 µM, and 1.65 µM, while the LOQ was found as 8.64 µM, 5.7 µM, and 5.01 µM in CV, DPV, and SWV, respectively, for the detection of ciprofloxacin.

在现代世界，抗生素的使用对诱发抗菌素耐药性（AMR）构成了严重威胁，这关系到可持续发展目标（sdg）。氟喹诺酮类抗生素，即恩诺沙星和环丙沙星，是破坏“同一个健康”的主要原因。在此背景下，为了提供一种环保和可靠的方法，我们用氧化碳酸镧（La2O2CO3）纳米粒子制作了一种绿色电化学传感器，用于检测几种复杂基质中的恩诺沙星和环丙沙星，这些基质与食品安全、环境监测和人类健康有关。采用绿色合成方法合成碳酸氧材料，同时利用芦笋叶中的植物化学物质发挥还原剂和封盖剂的作用。为了表征合成材料的结晶度和形貌，采用了多种光谱（XRD， BET， EDAX）和微观（FESEM， TEM）技术。用原子力显微镜（AFM）、角形仪和表面轮廓仪对制备的电极进行了表征。所制备的伏安传感器在恩诺沙星和环丙沙星的有效线性范围分别为2.77µM ~ 158.99µM和5.98µM ~ 57.87µM。在这两种情况下，循环伏安法（CV）、差分脉冲伏安法（DPV）和方波伏安法（SWV）都被用作电化学分析技术。恩诺沙星的检出限（LOD）分别为4.07µM、2.91µM和4.98µM，定量限（LOQ）分别为14.2µM、8.83µM和15.1µM。另一方面，环丙沙星在CV、DPV和SWV中的LOQ分别为2.85µM、1.90µM和1.65µM， LOQ分别为8.64µM、5.7µM和5.01µM。

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引用次数: 0

A fast, reproducible and sensitive human-plasma HPLC-fluorescence method to quantify Carvedilol and the error function 一种快速、重复性好、灵敏的人血浆hplc -荧光定量卡维地洛及其误差函数的方法

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-08-29 DOI: 10.1016/j.talo.2025.100544

Antonio J. Braza, Montserrat Viñas-Bastart, Maria Sureda-Rosich, Cecilia F. Lastra, Eduardo L. Mariño, Pilar Modamio

A robust and sensitive bioanalytical method was developed and fully validated using reverse-phase High-Performance Liquid Chromatography (HPLC) with fluorometric detection for the quantitative analysis of carvedilol in a complex matrix (human plasma). The method demonstrated satisfactory performance within the therapeutic concentration range (3.91–125 ng/mL), showing good linearity (coefficient of variation, CV: 12.05 %), accuracy (relative error, RE: 11.51 %), and precision—both intra-day (CV: 8.31 %) and inter-day (CV: 10.84 %)—as well as a recovery rate of 89.3 %. The lower limit of quantification (LLOQ) and the limit of detection (LOD) were 1.95 ng/mL and 0.98 ng/mL, respectively. Sample stability was confirmed for up to 24 h under autosampler conditions. The method yielded a short retention time of 3.15 minutes, enabling high-throughput analysis. Once validated, an analytical error function, expressed as a standard deviation (SD), was calculated to determine the most appropriate data-weighting approach across the calibration range. The best-fitting function was linear: SD (ng/mL) = 0.322 + 0.086C. An Analytical GREEnness Metric Approach (AGREE) of the method obtained a score of 0.67, indicating a moderately green profile. Due to its simplicity, cost-effectiveness, and rapid turnaround, the method is well suited for routine clinical use and may serve as a practical tool for monitoring medication adherence in healthcare settings, including hospitals.

建立了一种可靠、灵敏的生物分析方法，并充分验证了反相高效液相色谱（HPLC）与荧光检测对复杂基质（人血浆）中卡维地洛的定量分析。该方法在治疗浓度范围（3.91 ~ 125 ng/mL）内具有良好的线性（变异系数：12.05%）、准确度（相对误差：11.51%）、精密度(日内（CV: 8.31%）和日间（CV: 10.84%），回收率为89.3%。定量下限和检出限分别为1.95 ng/mL和0.98 ng/mL。在自动进样器条件下，样品稳定性可达24小时。该方法保留时间短，为3.15分钟，可实现高通量分析。验证后，计算以标准差（SD）表示的分析误差函数，以确定整个校准范围内最合适的数据加权方法。最佳拟合函数为线性：SD (ng/mL) = 0.322 + 0.086C。该方法的分析绿色度量方法（AGREE）得分为0.67，表明适度绿色。由于其简单，成本效益和快速周转，该方法非常适合常规临床使用，并可作为监测医疗机构（包括医院）药物依从性的实用工具。

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引用次数: 0

Application of BSA-modified silica for solid phase extraction of progesterone from river water samples bsa改性二氧化硅固相萃取河水中黄体酮的应用

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-08-29 DOI: 10.1016/j.talo.2025.100545

Mladen M. Đurđević , Miloš P. Pešić , Tatjana Ž. Verbić

This study reports the development and application of bovine serum albumin (BSA)-modified silica gels as selective and durable sorbents for the solid-phase extraction (SPE) of progesterone from river water samples. One of the sorbents was used for SPE preconcentration followed by progesterone quantification in a sample collected from the Danube River. The standard addition method followed by high-performance liquid chromatography (HPLC-DAD) detection enabled the quantification of progesterone at a concentration of 143.0 ± 4.0 ng/L. At elevated progesterone concentrations (1.0 to 2.0 × 10^-7 M), recoveries approached 100 %, demonstrating the high efficiency of the developed extraction sorbents. The overall method recovery ranged from 91.4 to 101.0 %, confirming the method’s accuracy and robustness. Furthermore, under optimized SPE conditions, developed sorbents were highly selective towards progesterone compared to other reproductive hormones. The sorbents exhibited excellent long-term stability, with no significant change in binding efficiency observed over a four-year period (variation lower than 3 %), maintaining consistent recoveries of 94–96 %. Newly developed BSA-modified silica sorbents represent a promising tool for trace analysis of steroid hormones, as well as for their routine environmental monitoring.

本研究报道了牛血清白蛋白（BSA）修饰硅胶作为固相萃取（SPE）河水中黄体酮的选择性和耐用吸附剂的开发和应用。其中一种吸附剂用于多瑙河样品的固相萃取预富集和黄体酮定量。采用标准加入-高效液相色谱法（HPLC-DAD）检测，测定黄体酮浓度为143.0±4.0 ng/L。当黄体酮浓度升高（1.0 ~ 2.0 × 10-7 M）时，回收率接近100%，表明所开发的萃取吸附剂具有较高的效率。总回收率为91.4% ~ 101.0%，证实了该方法的准确性和稳健性。此外，在优化的固相萃取条件下，制备的吸附剂对黄体酮的选择性高于其他生殖激素。吸附剂表现出优异的长期稳定性，在四年的时间内，结合效率没有显著变化（变化低于3%），回收率保持在94 - 96%之间。新开发的bsa改性二氧化硅吸附剂是一种很有前途的工具，用于类固醇激素的痕量分析，以及它们的常规环境监测。

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引用次数: 0

Fabrication of patterned paper-based sensors for highly efficient formaldehyde detection 用于高效甲醛检测的图案纸传感器的制造

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-08-28 DOI: 10.1016/j.talo.2025.100541

Taeuk Ji , Gwangbeom Nam , Shinwon Hwang, Minju Park, Jung Hwal Shin

Paper-based sensors are widely used in formaldehyde (FA) sensing in various studies. The development of paper-based sensors with high accuracy and rapid response is important for FA detection. In this study, paper-based FA sensors (PFASs) were developed using a commercial inkjet printer, with filter paper as a substrate. The substrate was coated with a reactive solution containing two pH indicators: thymol blue (TB) and bromophenol blue (BPB), and hydroxylamine sulfate. To evaluate the performance of PFASs, the intensity of the color change caused by FA vapor was detected via colorimetry and optimized with respect to the TB:BPB ratio of the solution and the pattern formation during printing. The optimum TB:BPB ratio was 10:1. The response time decreased as the pattern line width decreased and the pore size increased, and check patterns showed a faster response than those of regularly spaced lines. The sensor was tested in an environment with a relative humidity of 50 ± 5 % at room temperature to ensure consistent experimental conditions, minimizing any potential interference from external factors. The color change of the sensor was visibly noticeable for FA concentrations above 1 ppm. Thus, the proposed technique shows great promise for rapid, high-precision FA detection.

纸基传感器广泛应用于甲醛（FA）的检测中。开发高精度、快速响应的纸基传感器对FA检测具有重要意义。在本研究中，利用商用喷墨打印机，以滤纸为基材，开发了基于纸张的FA传感器（PFASs）。底物包被含有两种pH指示剂的反应溶液：百里酚蓝（TB）和溴酚蓝（BPB），以及硫酸羟胺。为了评价PFASs的性能，通过比色法检测FA蒸气引起的颜色变化强度，并根据溶液的TB:BPB比和印刷过程中的图案形成进行优化。最佳TB:BPB比为10:1。随着纹线宽度的减小和孔径的增大，响应时间减小，且格子纹的响应速度快于规则间距纹的响应速度。为了保证实验条件的一致性，最大限度地减少外部因素的潜在干扰，传感器在室温下的相对湿度为50±5%的环境中进行测试。当FA浓度高于1ppm时，传感器的颜色变化明显。因此，该技术对快速、高精度的FA检测具有很大的前景。

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引用次数: 0

Green rapid HPLC method for testing retinol and tocopherol in ophthalmic gels 绿色快速高效液相色谱法测定眼用凝胶中视黄醇和生育酚

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-08-28 DOI: 10.1016/j.talo.2025.100538

Xiao-Chen Huang , Ahmed Sobhy Darwish , Wageh Sobhy Darwish , Ri-Meng Chen , Jin-Kui Ma

In addition to providing immediate comfort for dry and irritated eyes, vitamin A (retinol) and E (tocopherol) drops strengthen the eyes' defenses. Optic gels, which are compatible with contact lenses, use vitamins A and E to improve vision, soothe and hydrate dry and sensitive eyes, and provide immediate comfort. It is now feasible to determine the amounts of vitamin A and vitamin E in ocular gels thanks to the development and validation of a thorough, rapid, simple, and eco-friendly stability-indicating LC approach. In compliance with ICH guidelines, the two medications underwent a series of assessments. Vitamin A palmitate, vitamin E acetate, and related degradants were successfully resolved via isocratic separation. Utilizing the octadecylsilyl phase at ambient temperature (25° C ± 5°C), both drugs were quantified and qualified. Constant ratios of filtered water (neutral purified water at pH range 5 to 7) to ethanol comprise the isocratic mobile phase, which has a flow rate of 2.0 mL/min. During the 290 nm detection, the concentrations of vitamin A palmitate and vitamin E acetate varied from 5 to 200 IU/mL and 25 to 1000 μg/mL, respectively. The LC technique's ecological benefits are demonstrated by its overall AES grade of 79, AGREE grade of 0.64, MoGAPI grade of 76, BAGI grade of 80, and final whiteness of 95.8. According to research, the suggested method is accurate, reliable, long-lasting, eco-friendly, and distinctive.

除了为干燥和受刺激的眼睛提供即时的舒适外，维生素A（视黄醇）和E（生育酚）滴剂还能增强眼睛的防御能力。光学凝胶，与隐形眼镜兼容，含有维生素A和E，改善视力，舒缓和滋润干燥和敏感的眼睛，并提供即时的舒适。由于开发和验证了一种彻底、快速、简单、环保的稳定性指示LC方法，现在可以测定眼凝胶中维生素A和维生素E的含量。根据ICH指南，对这两种药物进行了一系列评估。维生素A棕榈酸酯、维生素E醋酸酯和相关的降解物通过等温分离成功地分离出来。在室温（25°C±5°C）下，利用十八烷基硅基相对两种药物进行定量鉴定。过滤水（pH值范围为5至7的中性纯净水）与乙醇的恒定比例组成等密度流动相，其流速为2.0 mL/min。在290 nm检测过程中，维生素A棕榈酸酯和维生素E醋酸酯的浓度分别在5 ~ 200 IU/mL和25 ~ 1000 μg/mL之间变化。LC技术的总体AES等级为79，AGREE等级为0.64，MoGAPI等级为76，BAGI等级为80，最终白度为95.8，证明了LC技术的生态效益。研究表明，该方法准确、可靠、持久、环保、独特。

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引用次数: 0