Talanta Open最新文献_第6页

A ratiometric NIR fluorescent probe for simultaneous detection of H2S and viscosity: Applications in environmental monitoring and bioimaging 同时检测H2S和黏度的比例近红外荧光探针：在环境监测和生物成像中的应用

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-23 DOI: 10.1016/j.talo.2025.100564

Sha Li, Qin Li, Zhihui Yang, Wei Wen, Miao Yan, Haixian Ren

Hydrogen sulfide (H₂S), a critical gasotransmitter governing physiological functions, presents significant environmental and food safety hazards at aberrant concentrations. Concurrently, mitochondrial viscosity serves as a vital biomarker for cellular pathologies. To address the unmet need for precise dual-parameter monitoring, we engineered a mitochondria-targeted near-infrared (NIR) ratiometric fluorescent probe, T-N, enabling simultaneous and discriminative detection of H₂S and viscosity through discrete emission channels. The probe exhibits ultrarapid response (<30 s), exceptional sensitivity, and superb selectivity against competing analytes. Leveraging H₂S-triggered chromogenic shifts, T-N-immobilized test strips facilitate instrument-free, semi-quantitative assessment of food spoilage, validated across environmental waters and industrially relevant beer samples. Moreover, T-N achieves synchronous dual-channel imaging of endogenous/exogenous H₂S and viscosity dynamics in live cells, with verified mitochondrial localization. This work establishes a versatile sensing platform for tracking H₂S and viscosity in interconnected environmental, food safety, and biomedical contexts.

硫化氢（H2S）是一种控制生理功能的重要气体递质，在异常浓度下会对环境和食品安全造成重大危害。同时，线粒体粘度作为细胞病理的重要生物标志物。为了满足精确双参数监测的需求，我们设计了一种针对线粒体的近红外（NIR）比例荧光探针T-N，可以通过离散发射通道同时鉴别检测H2S和粘度。该探针具有超快速响应（30秒），卓越的灵敏度和对竞争分析物的极好选择性。利用h2s触发的显色变化，t- n固定试纸可以实现无仪器、半定量的食品腐败评估，并在环境水域和工业相关的啤酒样品中得到验证。此外，T-N在活细胞中实现了内源性/外源性H2S和黏度动力学的同步双通道成像，并验证了线粒体定位。这项工作建立了一个多功能传感平台，用于在相互关联的环境、食品安全和生物医学环境中跟踪H2S和粘度。

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引用次数: 0

Electrochemical sensors for the determination of polyphenols as antioxidants from natural sources: A comprehensive review of sensor development and characterization 电化学传感器用于测定天然来源的抗氧化剂多酚：传感器发展和表征的综合综述

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-22 DOI: 10.1016/j.talo.2025.100562

Soner Çakar , Mahmut Özacar

The rapid, sensitive, and selective quantification of polyphenols has become increasingly important in areas such as food quality control, nutraceutical development, and biomedical diagnostics, due to their exhibiting significant antioxidant properties through their redox-active hydroxyl groups, contributing to a variety of pharmacological effects, including anti-inflammatory, cardioprotective, and chemopreventive activities. This review critically examines recent developments in electrochemical sensor platforms tailored for polyphenol detection in complex biological and environmental samples. Emphasis is placed on nanostructured electrode modifications employing carbon-based nanomaterials (e.g., graphene, carbon nanotubes), metal nanoparticles (Au, Ag, Pt), and metal oxide nanostructures (e.g., TiO₂, ZnO) that facilitate enhanced electron transfer rates, augmented electroactive surface area, and improved sensor stability and reproducibility. The review further explores diverse electrochemical transduction techniques, including cyclic voltammetry, square wave voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy, with comparisons of enzymatic and non-enzymatic sensing approaches. Detailed mechanistic insights into polyphenol electrooxidation pathways, adsorption phenomena, and sensor–analyte interfacial interactions are discussed. Complementary spectroscopic and microscopic characterization methods are highlighted for their roles in elucidating structural, electronic, and surface properties critical to sensor functionality and analytical performance.

多酚通过其氧化还原活性羟基表现出显著的抗氧化特性，有助于多种药理作用，包括抗炎、心脏保护和化学预防活性，因此多酚的快速、敏感和选择性定量在食品质量控制、营养制剂开发和生物医学诊断等领域变得越来越重要。本文综述了电化学传感器平台在复杂生物和环境样品中多酚检测方面的最新进展。重点放在纳米结构电极修饰，采用碳基纳米材料（如石墨烯，碳纳米管），金属纳米颗粒（Au, Ag, Pt）和金属氧化物纳米结构（如TiO₂，ZnO），促进提高电子转移速率，增加电活性表面积，提高传感器的稳定性和可重复性。本文进一步探讨了多种电化学转导技术，包括循环伏安法、方波伏安法、差分脉冲伏安法和电化学阻抗谱，并对酶和非酶传感方法进行了比较。详细的机制见解多酚电氧化途径，吸附现象和传感器-分析物界面的相互作用进行了讨论。互补的光谱和微观表征方法在阐明结构、电子和表面特性方面的作用，对传感器功能和分析性能至关重要。

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引用次数: 0

Rapid forensic characterization of automotive clear coats by ATR-FTIR combined with chemometrics ATR-FTIR结合化学计量学对汽车透明涂层的快速法医鉴定

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-22 DOI: 10.1016/j.talo.2025.100563

Polina M. Ilicheva , Kristina T. Musina , Sergey P. Kurchatkin , Natalia A. Burmistrova , Yulia B. Monakhova

Hit-and-run criminal behavior is a challenge for law enforcement agencies around the world. Paint chips left at a crime scene become important for the criminal investigation process. The clear coat is of great interest since it is most likely to be transferred through contact and therefore found at the crime scene. Fourier transform infrared spectroscopy with attenuated total reflectance (ATR-FTIR) spectroscopy combined with chemometric tools is shown to efficient for forensic examination of clear coats. The possibility of improving the chemometric model generalizability was shown through a dataset encompassing diverse 125 samples. The chemical signatures of melamine cross‐linking and urethane were observed during investigation of spectral data of individual clear coats. The best exploratory principal components analysis (PCA) model was constructed with the first four principal components (PCs), that described 90.3 % of the total data variance. Supervised model classification using partial least squares – discriminant analysis (PLS-DA) showed ssensitivity (SEN) and specificity (SPC) of 95 % and 100 % for external test set (overall accuracy was 92 %). Independent components analysis (ICA) model with 6 independent components (ICs) was used to interpret chemical features responsible for class separation. High predictive ability and interpretability of multivariate approach demonstrates the potential of ATR-FTIR spectroscopy and chemometrics for characterization of automotive clear coats of various manufacturers and models.

肇事逃逸的犯罪行为对世界各地的执法机构来说都是一个挑战。犯罪现场留下的油漆碎片对刑事调查过程非常重要。这件透明外套引起了极大的兴趣，因为它很可能是通过接触转移的，因此在犯罪现场被发现。傅里叶变换红外光谱与衰减全反射（ATR-FTIR）光谱结合化学计量工具被证明是有效的法医检查透明涂层。通过包含125个不同样本的数据集显示了提高化学计量模型泛化性的可能性。通过对单个透明涂层光谱数据的研究，观察到三聚氰胺交联和聚氨酯的化学特征。最佳的探索性主成分分析（PCA）模型是由前四个主成分（PCs）构建的，它们描述了总数据方差的90.3%。使用偏最小二乘判别分析（PLS-DA）的监督模型分类显示，外部测试集的敏感性（SEN）和特异性（SPC）分别为95%和100%（总体准确率为92%）。采用具有6个独立成分的独立成分分析（ICA）模型来解释导致类分离的化学特征。多元方法的高预测能力和可解释性证明了ATR-FTIR光谱和化学计量学在不同制造商和车型的汽车透明涂层表征方面的潜力。

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引用次数: 0

Advancement in electrochemical sensing of chloramphenicol in varying matrixes: A review 氯霉素在不同基质中的电化学传感研究进展

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-20 DOI: 10.1016/j.talo.2025.100561

Kgwadu Percy Mulaudji, Kefilwe Vanessa Mokwebo, Franklin Quelain De Bruin, Keagan Pokpas, Natasha Ross

Chloramphenicol (CAP) have demonstrated its broad effectiveness against bacterial infections. However, its persistence in the environment and potential toxicity led to strict global regulations limiting its use. As a result, rapid and accurate detection methods for CAP are being developed to protect public health and maintain regulatory compliance regarding its presence in the environment. Conventional analytical methods such as High-Performance Liquid Chromatography, Gas Chromatography, and Liquid Chromatography–Mass Spectrometry were commonly used for CAP detection. However, these conventional methods suffer challenges such as time-consumption, fabrication complexity, reproducibility, and cost. With electrochemical sensing techniques presenting varying valuable benefits, such as instant detection, low power consumption, simultaneous analysis, and portability, making them essential for timely monitoring of various analytes. The performance of electrochemical sensor is further boosted by integrating materials such as spinels, metal oxides, and metallic nanoparticles. These materials are broadly investigated as electrode interface components thanks to their features that collectively boost electrochemical sensor such as providing excellent electrical conductivity, distinct catalytic behavior, and larger surface areas. Furthermore, this paper reviews the impact of traditional and standards analytical methods, as well as their recent advancement based on the detection of CAP in aqueous media.

氯霉素（CAP）已被证明对细菌感染具有广泛的有效性。然而，它在环境中的持久性和潜在的毒性导致严格的全球法规限制其使用。因此，正在开发快速和准确的CAP检测方法，以保护公众健康并保持其在环境中存在的法规遵从性。高效液相色谱法、气相色谱法、液相色谱-质谱法等常规分析方法是CAP检测常用的方法。然而，这些传统方法面临着诸如耗时、制造复杂性、可重复性和成本等挑战。随着电化学传感技术呈现出各种有价值的优点，如即时检测，低功耗，同时分析和便携性，使其成为及时监测各种分析物的必要条件。尖晶石、金属氧化物、金属纳米颗粒等材料的集成进一步提高了电化学传感器的性能。这些材料作为电极界面组件被广泛研究，这要归功于它们共同提升电化学传感器的特性，如提供优异的导电性、独特的催化行为和更大的表面积。此外，本文综述了传统分析方法和标准分析方法的影响，以及基于水介质中CAP检测的最新进展。

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引用次数: 0

Eco-friendly HPLC method for simultaneous determination of pantoprazole and domperidone: Comprehensive evaluation of greenness, whiteness, and blueness 环保高效液相色谱法同时测定泮托拉唑和多潘立酮的绿、白、蓝综合评价

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-15 DOI: 10.1016/j.talo.2025.100560

Aya Barseem , Reem H. Obaydo , Samar H. Elagamy

An environmentally friendly RP-HPLC method was developed for the simultaneous determination of pantoprazole (PAN) and domperidone (DOM). Chromatographic separation was performed using isocratic elution on CORTECS® Shield RP18 column maintained at 25 °C. The mobile phase consisted of ethanol and potassium phosphate buffer (50.0 mM, pH 6.0) in a 30:70 (v/v) ratio, delivered at a flow rate of 1.0 mL/min, with UV detection at 286 nm. The method demonstrated excellent linearity within the concentration ranges of 1.0–25.0 μg/mL for PAN and 3.0–30.0 μg/mL for DOM. It was successfully applied to the analysis of both drugs in bulk and pharmaceutical dosage forms. The greenness of the proposed method was evaluated using the Analytical Eco-Scale, yielding a total score of 83, which indicates minimal environmental impact. The method "whiteness" was assessed using the RGB 12 model, resulting in a high total score of 89.2, reflecting a well-balanced combination of analytical performance, environmental sustainability, and practical implementation. Its "blueness," representing practical applicability, was measured using the BAGI tool and achieved a total score of 82.5. Compared to a previously reported HPLC method, the proposed method offers comparable practical applicability while exhibiting enhanced greenness through the use of ethanol as the organic modifier, rather than environmentally hazardous solvents such as acetonitrile and methanol. The method was fully validated according to ICH Q2(R1) guidelines, confirming its accuracy, precision, specificity, and robustness. Furthermore, the proposed method was statistically compared with a previously reported HPLC method using Student’s t-test and F-test.

建立了同时测定泮托拉唑（PAN）和多潘立酮（DOM）含量的环保反相高效液相色谱法。色谱分离采用CORTECS®Shield RP18色谱柱等密度洗脱，保持在25°C。流动相为乙醇和磷酸钾缓冲液（50.0 mM, pH 6.0），体积比为30:70，流速为1.0 mL/min，紫外检测波长为286 nm。PAN在1.0 ~ 25.0 μg/mL、DOM在3.0 ~ 30.0 μg/mL范围内呈良好的线性关系。它成功地应用于原料药和药物剂型的分析。使用分析生态尺度对所提出方法的绿色度进行了评估，得到总分83分，这表明对环境的影响最小。“白度”方法采用RGB 12模型进行评估，得到89.2分的高分，反映了分析性能、环境可持续性和实际实施的良好平衡。它的“蓝度”，代表实用性，是用BAGI工具测量的，得到了82.5分的总分。与先前报道的HPLC方法相比，该方法具有相当的实用性，同时通过使用乙醇作为有机改性剂而不是对环境有害的溶剂（如乙腈和甲醇），显示出更高的绿色。根据ICH Q2（R1）指南对该方法进行了充分验证，确认了其准确性、精密度、特异性和稳健性。此外，采用学生t检验和f检验对所提出的方法与先前报道的HPLC方法进行了统计比较。

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引用次数: 0

A simple and high-throughput ICP-MS method for the determination of 46 elements in urine: Biomonitoring of occupational exposure in automotive workers 一种简单、高通量ICP-MS测定尿液中46种元素的方法：汽车工人职业暴露的生物监测

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-14 DOI: 10.1016/j.talo.2025.100559

Ruirui Dai , Huadong Zhang , Wei Yang , Xiaoxi Wang , Xiaoping Li , Yujie Yu , Fengqiong Chen

Monitoring urinary metal concentrations is crucial for assessing occupational exposure and associated health risks. Inductively coupled plasma mass spectrometry (ICP-MS) is widely considered the method of choice for multi-element analysis due to its high sensitivity, low detection limits, and broad dynamic range. This study presents a simple and rapid ICP-MS method for the simultaneous determination of 46 elements in urine, utilizing 2 % nitric acid and 1.5 % ethanol (v/v) for sample pretreatment. This approach effectively mitigates matrix effects and carbon-based interferences. The method demonstrated excellent linearity (coefficient of determination, R² ≥ 0.9985), precision (relative standard deviation, RSD < 8 %), and accuracy (recoveries 80–120 %). The method was applied to analyze 268 urine samples from automotive manufacturing workers. Several elements of occupational relevance, including Nickel (Ni), tungsten (W), molybdenum (Mo), titanium (Ti) and Cadmium (Cd), as well as essential elements such as iron (Fe), copper (Cu), and zinc (Zn), were elevated compared with general population references, indicating complex multi-metal exposure profiles. This work provides a validated and efficient tool for occupational biomonitoring and delivers valuable elementomic data to support exposure assessment, workplace interventions, and future research on the health effects of multi-metal exposure.

监测尿中金属浓度对于评估职业接触和相关健康风险至关重要。电感耦合等离子体质谱法（ICP-MS）因其灵敏度高、检出限低、动态范围宽等优点被广泛认为是多元素分析的首选方法。采用2%硝酸和1.5%乙醇（v/v）进行样品预处理，建立了简便、快速的ICP-MS同时测定尿液中46种元素的方法。这种方法有效地减轻了基体效应和碳基干扰。该方法具有良好的线性（决定系数，R²≥0.9985）、精密度（相对标准偏差，RSD < 8%）和准确度（加样回收率80 ~ 120%）。该方法应用于268份汽车制造工人的尿样分析。与一般人群相比，与职业相关的几种元素，包括镍（Ni）、钨(W)、钼（Mo）、钛（Ti）和镉（Cd），以及必需元素，如铁（Fe）、铜（Cu）和锌（Zn），都有所升高，表明复杂的多金属暴露谱。这项工作为职业生物监测提供了一个有效的工具，并提供了有价值的元素数据，以支持暴露评估、工作场所干预和未来多金属暴露对健康影响的研究。

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引用次数: 0

Analytical methodologies for antioxidant capacity assessment: An updated review 抗氧化能力评估的分析方法：最新综述

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-14 DOI: 10.1016/j.talo.2025.100558

Mahmoud Hamed , Faisal K. Algethami , Alaa Bedair , Fotouh R. Mansour

Antioxidants are crucial molecules protecting biological systems from harmful oxidation reactions and free radicals. This class of compounds attracted remarkable attention for their potential in functional foods and dietary supplements. The imbalance between oxidants and antioxidants leads to oxidative stress, associated with various chronic diseases. Accurate measurement of antioxidant activity is therefore essential for evaluating potential health-enhancing agents in food science, medicine, and biotechnology. This review provides a comprehensive overview of contemporary analytical methodologies for assessing antioxidant capacity across food, cosmetic, pharmaceutical, and biomedical applications, exploring four major categories of techniques: spectrophotometric methods, including UV–Vis, electron paramagnetic resonance (EPR), and near infrared (NIR) spectroscopy, highlighting their principles and applications in radical scavenging assays and antioxidant characterization. Electrochemical techniques are rapid, sensitive, and cost-effective alternatives, focusing on voltammetry, amperometry, and biosensor development for direct measurement of electron transfer processes. The role of biosensors, employing bioreceptors like enzymes and DNA, for selective and sensitive antioxidant detection is also examined. Chromatographic methods, such as high-performance liquid chromatography (HPLC) and gas chromatography (GC), paired with selective detectors, are used to separate, identify, and quantify antioxidant compounds in complex matrices. This review highlights the principles, advancements, advantages, and limitations of these diverse methodologies in the accurate evaluation of antioxidant potential.

抗氧化剂是保护生物系统免受有害氧化反应和自由基侵害的关键分子。这类化合物因其在功能性食品和膳食补充剂中的潜力而备受关注。氧化剂和抗氧化剂之间的不平衡导致氧化应激，与各种慢性疾病有关。因此，准确测量抗氧化活性对于评估食品科学、医学和生物技术中潜在的健康促进剂至关重要。本文综述了食品、化妆品、制药和生物医学领域抗氧化能力评估的现代分析方法，探讨了四大类技术：分光光度法，包括紫外-可见、电子顺磁共振（EPR）和近红外（NIR）光谱，重点介绍了它们在自由基清除试验和抗氧化表征中的原理和应用。电化学技术是一种快速、灵敏、经济的替代方法，主要集中在伏安法、安培法和生物传感器的开发上，用于直接测量电子转移过程。生物传感器的作用，采用生物受体如酶和DNA，选择性和敏感的抗氧化剂检测也进行了检查。色谱方法，如高效液相色谱（HPLC）和气相色谱（GC），与选择性检测器配对，用于分离、鉴定和定量复杂基质中的抗氧化化合物。本文综述了这些不同方法在准确评价抗氧化潜力方面的原理、进展、优势和局限性。

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引用次数: 0

Unveiling the role of α- and β-CDs in gold nanoparticle gel-based sensors for Fe³⁺ colorimetric detection 揭示α-和β-CDs在用于Fe +比色检测的金纳米颗粒凝胶传感器中的作用

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-13 DOI: 10.1016/j.talo.2025.100557

Agustina Sus Andreani , Farrah Nurkhaliza , Muhammad Ridwan , Salsabila Freya Daniarti , Lilis Rosmaniar , Meiyanti Ratna Kumalasari

The detection of Fe³⁺ in water samples is critical for mitigating potential harm to environmental and human health. This study presents a comparative investigation of α-cyclodextrin (α-CDs) and β-cyclodextrin (β-CDs) as stabilizing agents in the development of gold nanoparticle (AuNPs)-based gel sensors for Fe³⁺ colorimetric sensing. The AuNPs were synthesized using ortho-hydroxybenzoic acid (o-HBA) as a reducing agent. The red-wine colloidal solutions of AuNPs-o-αCDs and AuNPs-o-βCDs were measured by a UV–Vis spectrophotometer, showing a surface plasmon resonance (SPR) band at 517 nm for both. Field emission scanning electron microscopy – energy dispersive X-ray (FESEM-EDX) analysis revealed well-dispersed AuNPs within the gel matrix. Particle size analysis (PSA) and zeta potential measurements further indicated a smaller particle size and superior colloidal stability in the AuNPs-o-βCDs. The analytical performance of both sensors was evaluated through precision, accuracy (%Recovery), and comparison with atomic absorption spectroscopy (AAS) via t-test assessments. Both sensors demonstrated acceptable precision and accuracy, showing no statistically significant difference from AAS results (p > 0.05). Notably, the gel-AuNPs-o-βCDs sensor exhibited enhanced sensitivity (0.20 mg/L) and faster detection, achieving stable readings within 15 min. In contrast, the gel-AuNPs-o-αCDs had a LoD of 0.57 mg/L and required 20 min for stable readout. Additionally, the gel-AuNPs-o-βCDs demonstrated a higher linear correlation with Fe³⁺ concentration (R² = 0.9999) than the gel-AuNPs-o-αCDs (R² = 0.9994). These findings suggested that β-CDs provide superior stabilization and enhanced performance in the gel-based AuNPs sensors, establishing them as a highly promising candidate for rapid, reliable, and accurate Fe³⁺ detection in tap water.

水样中Fe3+的检测对于减轻对环境和人类健康的潜在危害至关重要。本研究比较了α-环糊精（α-CDs）和β-环糊精（β-CDs）作为稳定剂在制备纳米金（AuNPs）凝胶传感器中对Fe3+比色的影响。以邻羟基苯甲酸（o-HBA）为还原剂合成了AuNPs。用紫外-可见分光光度计测定了AuNPs-o-αCDs和AuNPs-o-βCDs的红酒胶体溶液，两者在517 nm处均有表面等离子体共振（SPR）带。场发射扫描电子显微镜-能量色散x射线（FESEM-EDX）分析显示凝胶基质中分布良好的aunp。粒径分析（PSA）和zeta电位测量进一步表明，AuNPs-o-βCDs具有更小的粒径和更好的胶体稳定性。两种传感器的分析性能通过精密度、准确度（回收率%）和通过t检验评估与原子吸收光谱（AAS）的比较来评估。两种传感器的精度和准确度均可接受，与原子吸收光谱结果无统计学差异（p > 0.05）。值得注意的是，凝胶- aunps -o-βCDs传感器具有更高的灵敏度（0.20 mg/L）和更快的检测速度，可在15分钟内获得稳定读数。相比之下，凝胶- aunps -o-αCDs的LoD为0.57 mg/L，需要20 min才能稳定读数。此外，凝胶- aunps -o-βCDs与Fe3+浓度的线性相关（R2 = 0.9999）高于凝胶- aunps -o-αCDs （R2 = 0.9994）。这些发现表明，β-CDs在凝胶型AuNPs传感器中提供了优越的稳定性和增强的性能，使其成为快速、可靠和准确检测自来水中Fe3+的极有希望的候选材料。

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引用次数: 0

Stability and reproducibility study for the development of a potentiometric nitrate sensor for in-situ use 原位使用电位式硝酸盐传感器的稳定性和重复性研究

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-11 DOI: 10.1016/j.talo.2025.100556

Michelle Brandão Silva de Assis , Kathrin Trommer , Alfred Kick , Michael Mertig

This study investigates the stability and reproducibility of a potentiometric nitrate sensor in all-solid-state configuration, consisting of a screen-printed graphite electrode, coated with electropolymerized polypyrrole as solid contact material, and covered by a TDMA-based ion-selective membrane. Special attention is given to the long-term stability, depending on storage and conditioning conditions as the most important factors for the applicability of the sensor. In particular, regression lines from calibration procedures, performed over a period of up to three months, were analyzed to evaluate the sensor performance. The sensor demonstrated superior stability, with minimal, nearly parallel shifts between regression lines. Notably, the sensor retained its ability to reproduce signals accurately even after one-month periods of dry storage, provided that the applied conditioning period was sufficiently long. The sensor was successfully applied for nitrate detection in drinking water samples, with a reproducibility of ± 3 mg/L, making it a promising candidate for real-time nitrate sensing applications. In all stages of the experiments, the sensor performance was compared with that of an all-solid-state sensor system, consisting of a gold electrode coated with poly(3-octylthiophene-2,5-diyl) and molybdenum disulfide nanocomposites as solid contact material.

本研究研究了一种全固态电位硝酸盐传感器的稳定性和再现性，该传感器由丝网印刷石墨电极组成，表面涂有电聚合聚吡咯作为固体接触材料，表面覆盖一层基于tdma的离子选择膜。特别注意的是长期稳定性，这取决于存储和调节条件作为传感器适用性的最重要因素。特别是，对校准过程中的回归线进行了长达三个月的分析，以评估传感器的性能。该传感器表现出优越的稳定性，在回归线之间有最小的几乎平行的位移。值得注意的是，即使在一个月的干燥储存期后，只要施加的调理期足够长，传感器仍能准确地再现信号。该传感器成功应用于饮用水样品的硝酸盐检测，重现性为±3 mg/L，是实时硝酸盐传感应用的理想选择。在实验的各个阶段，传感器的性能与全固态传感器系统的性能进行了比较，全固态传感器系统由涂有聚（3-辛基噻吩-2,5-二酰基）和二硫化钼纳米复合材料作为固体接触材料的金电极组成。

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引用次数: 0

SensEDNA: An innovative optical CRISPR platform for rapid environmental DNA monitoring SensEDNA：一个创新的光学CRISPR平台，用于快速环境DNA监测

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-10 DOI: 10.1016/j.talo.2025.100551

Molly Ann Williams , Ciprian Briciu-Burghina , Sean Power , Joyce O’Grady , Elvira de Eyto , Nigel Kent , Anne Parle-McDermott , Fiona Regan

Biodiversity loss is at an all-time high increasing the need for simple and rapid species monitoring systems. Management and conservation of fish species within aquatic environments requires knowledge of distribution, traditionally gained through visual detection and counting. These methods are expensive, time consuming and can lead to harm of the species of interest. Environmental DNA (eDNA) offers a solution to this through detection of DNA that may be shed into the environment by a given target species. Integrating eDNA based molecular assays to a biosensor device enables onsite sample testing for rapid species assessment.

Herein, a portable fluorometer with incubating capabilities (SensEDNA) was custom designed and built to conduct the simultaneous incubation and fluorescent detection steps required for isothermal eDNA detection. The system features three optical cells to enable triplicate analysis. Each optical cell is set to incubate at 37 °C and control within 0.5 °C. Fluorescence detection with excitation at 485 nm and emission at 535 nm was integrated and optimised to provide highest signal/noise ratio while minimising fluorescence bleaching. The system comprises a simple LED/high pass filter/photodiode configuration and is controlled by a Wixel board. This affordable and user-friendly optical detection platform, coupled with RPA-CRISPR-Cas, provides a crucial step towards on-site single species identification from eDNA.

生物多样性的丧失正处于空前的高度，增加了对简单和快速物种监测系统的需求。水生环境中鱼类的管理和保护需要有关分布的知识，传统上是通过目测和计数获得的。这些方法成本高，耗时长，而且可能导致对感兴趣的物种的伤害。环境DNA （eDNA）通过检测可能由给定目标物种释放到环境中的DNA提供了解决方案。将基于eDNA的分子分析集成到生物传感器设备中，可以进行现场样品测试，以进行快速物种评估。为此，我们定制了一种具有孵育功能的便携式荧光仪（SensEDNA），用于同时进行等温eDNA检测所需的孵育和荧光检测步骤。该系统具有三个光学单元，可以进行三次重复分析。每个光学细胞设置在37°C孵育，控制在0.5°C内。集成并优化了485 nm激发和535 nm发射的荧光检测，以提供最高的信噪比，同时最大限度地减少荧光漂白。该系统包括一个简单的LED/高通滤波器/光电二极管配置，并由一个Wixel板控制。这种价格合理且用户友好的光学检测平台，加上RPA-CRISPR-Cas，为eDNA的现场单物种鉴定提供了关键的一步。

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引用次数: 0