Talanta Open最新文献

{"title":"Identification of potential volatile markers for characterizing Argentine wine vinegars based on their production process","authors":"Marcelo Wagner ,&nbsp;Jorgelina Zaldarriaga Heredia ,&nbsp;M. Pilar Segura-Borrego ,&nbsp;M. Lourdes Morales ,&nbsp;José M. Camiña ,&nbsp;Silvana M. Azcarate ,&nbsp;Raquel M. Callejón ,&nbsp;Rocío Ríos-Reina","doi":"10.1016/j.talo.2024.100370","DOIUrl":"10.1016/j.talo.2024.100370","url":null,"abstract":"<div><div>In Argentina, the production of quality wine vinegars has been barely exploited. Currently, most of the marketed vinegars are produced using rapid industrial fermentation systems obtaining vinegars with a high production rate, but with low quality and few organoleptic nuances. This work aims to study the volatile profile by headspace solid phase microextraction (HS-SPME) coupled to gas chromatography-mass spectrometry (GC–MS) and chemometrics to differentiate Argentinian wine vinegars according to their production process (industrial or traditional). A total of 92 volatile compounds were identified on the samples by using a strategy based on volatile profile processing using PARADISe® software. The complete volatile profile of all the samples was submitted to partial least squares discriminant analysis (PLS-DA) for the selection of variables with importance in the projection (VIPs). Thus, 37 volatile compounds with the potential to be markers of the manufacturing process were selected. The results obtained revealed, for the first time, the volatile profile of Argentine wine vinegars showing that the compound groups that, on average, exhibited higher relative areas were esters, acids, and alcohols. Thus, certain acids, aldehydes and terpenes were noticeably present in industrial wine vinegar. Conversely, the alcohols were strongly associated with traditional ones. In the case of esters, they were connected to the different types of wine vinegars. These compounds could be considered as potential volatile markers of quality and authenticity of these types of vinegars.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100370"},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142533039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the greenness, blueness, and whiteness of spectroscopic and UPLC techniques for the simultaneous measurement of anti-glaucoma drugs and the preservation agent","authors":"Eman A. Assirey ,&nbsp;Noha S. katamesh ,&nbsp;Mahmoud A. Mohamed","doi":"10.1016/j.talo.2024.100367","DOIUrl":"10.1016/j.talo.2024.100367","url":null,"abstract":"<div><div>The pursuit of sustainable solutions is increasingly crucial in contemporary times. We propose inventive research that fulfills the criteria of whiteness and greenness in analytical chemistry. The endeavor aims to advance environmentally conscious techniques for the simultaneous detection of timolol maleate (TIM), dorzolamide HCl (DOR), and benzalkonium Cl (BNZ). The first method employed a sustainable mobile phase consisting of 0.02 M phosphate buffer: ethanol (68:32, v/v), and a flow rate of 0.6 mL/min with retention times 1.274, 1.798, and 2.483 min for DOR, TIM, and BNZ, respectively, with a UV detector at 210 nm. This methodology was easy, fast, and accurate. The approach showed low processing times, peak symmetry, and satisfactory resolution, with correlation values of 0.999. The linearity was between 0.001–0.064, 0.001–0.060 µg mL^-1, and 0.001–0.050 µg mL^-1for DOR, TIM, and BNZ, respectively. Furthermore, employing water as the solvent, complementary spectrophotometric methods such as Mean Centering of Ratio Spectra (MCR) and Successive Derivative Ratio Spectra (SRDS) were investigated as economical and environmentally friendly substitutes. All procedures demonstrated good linearity (r² &gt; 0.9990), acceptable accuracy, and precision (RSD ≤ 2 %). Furthermore, by proving the superiority of the current work over previously published methods regarding sustainability, analytical performance, economics, and practicality, the real-world implementation of four greenness and whiteness algorithms boosts the approval and overall sustainable development of the proposed methods. The suggested techniques offer low-cost, environmentally friendly substitutes for traditional methods, advancing the field of analytical chemistry towards more sustainable practices and fostering a less destructive future for quality control.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100367"},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142533040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Absolute mass spectrometry: A theoretical analytical technique for quantification","authors":"Ignacio J. Rios,&nbsp;Fabiana C. Gennari","doi":"10.1016/j.talo.2024.100366","DOIUrl":"10.1016/j.talo.2024.100366","url":null,"abstract":"<div><div>Mass spectrometry (MS) is an analytical chemistry technique that requires calibration with standards in order to quantify an analyte in a sample. In many cases the reference standards may be difficult to obtain or unavailable. In fact, when MS is applied to study reactions that yield multiple products, their quantification requires several standards and involves time-consuming and tedious calibration procedures. In other applications like Mass Spectrometry Imaging (MSI), quantification becomes even more tedious when on-tissue standards or mimetic tissue models are required. In this work, we introduce a new measurement technique that we called the Absolute Mass Spectrometry (AbMS), which allows the absolute quantification of an analyte without the need for calibration standards. This technique is based on the “Influence Method” developed in 2015 for nuclear measurements which, when applied to MS, produces this improvement for analyte quantification. Two possible applications of this technique are presented. One is the conceptual development of a mass spectrometer with electronic ionization that does not require calibration to determine the amount of analyte. The other one describes how to apply this technique in a mass spectrometer with other ionization sources like MALDI, DESI, and SIMS, which are used in MSI. AbMS technique uses the conceptual basis of Influence Method and two consecutive measurements to estimate the amount of analyte. Specifically, AbMS can be directly employed as an absolute method for quantification in MSI. Therefore, it constitutes an attractive complement for research applications in pharmaceutical and health sciences.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100366"},"PeriodicalIF":4.1,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142533038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A validated high-performance liquid chromatography-ultraviolet method for the determination of valproic acid derivatives in pharmaceutical formulations with a microbiological suitability evaluation","authors":"Abdullah H. Alluhayb ,&nbsp;Mostafa F. Al-Hakkani ,&nbsp;Mahmoud Abdulnasser ,&nbsp;Bandar R. Alsehli ,&nbsp;Mohammed S. Saddik ,&nbsp;Mohammad H.A. Hassan ,&nbsp;Sayed M Saleh","doi":"10.1016/j.talo.2024.100365","DOIUrl":"10.1016/j.talo.2024.100365","url":null,"abstract":"<div><div>Valproic acid and its derivatives are common drugs used in the treatment of epilepsy, a result of CNS disorders. Because the molecular structure of valproic acid, as a fatty acid, does not contain any chromophore groups, its peak response demanded special conditions to be detected in HPLC, ensuring the best precision results. Still, the current approach illustrates simple, validated procedures for conduction. The conducted chromatographic system consists of the BDS Hypersil C8, 150 × 4.6 mm, 5 µm column using a mobile phase of acetonitrile: phosphate buffer (4:6) at a detection wavelength of 215 nm at room temperature. A full method validation study was conducted and approved to ensure precise, repeatable, and accurate results by implementing system suitability parameters. In microbiological terms, a suitability test is used to validate microbiological testing methods, ensuring their efficacy despite potential interference from antimicrobial properties in the tested materials. The HPLC-UV developed and validated analytical method was evaluated, and it was found to be sensitive for use in the detection of low concentrations of methylparaben, propylparaben, and valproate with an optimum run time of six minutes. limit of detection (LODs) were statistically estimated and found to be 2.27 µg/mL, 39.77 ng/mL, and 1.84 µg/mL for methylparaben, propylparaben, and valproate, respectively. Additionally, the method demonstrated a high recovery of methylparaben, propylparaben, and valproate with an excellent closed-accuracy range (98.84% - 101.34%). An excellent regression coefficient (r) of 0.99924, 0.99998, and 0.99997 for methylparaben, valproate, and propylparaben, respectively, was achieved. Assay determination of various pharmaceutical dosage forms in the local market was implemented, yielding admirable results.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100365"},"PeriodicalIF":4.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development an automated and high -throughput analysis methods for detecting 64 anabolic androgenic steroids in urine using gas chromatography-mass spectrometry","authors":"Zhaobo Wu ,&nbsp;Aoxin Ma ,&nbsp;Lili Xing ,&nbsp;Xiaochen Huang ,&nbsp;Guojun Li ,&nbsp;Kaoqi Lian","doi":"10.1016/j.talo.2024.100362","DOIUrl":"10.1016/j.talo.2024.100362","url":null,"abstract":"<div><div>A gas chromatography tandem mass spectrometry (GC–MS/MS) assay was developed for the simultaneous detection of 64 anabolic androgenic steroids (AAS). Urine is extracted with tertbutyl methyl ether under alkaline conditions, purified with C18, and derived through silanization, Detection is carried out in dynamic multiple reaction monitoring (dMRM) mode, The overall recoveries ranged from 74.31 % to 119.66 %, The relative standard deviation is &lt;10 %, with a linear range of 1–200 ng/ml, The linear range of endogenous androsterone (A) and etiocholanolone (Etio) is 1.7–10,000 ng/ml, with a correlation coefficient 0.9991– 0.9999. The limit of detection (LOD) range of this experiment was 0.2 ng/ml-1.2 ng/ml, and limit of quantification (LOQ) was 0.7 ng/ml-4 ng/ml, The results of testing 100 actual samples show that this method is simple, effective and feasible, and can be used for the detection of AAS, meeting the requirements of the World Anti Doping Agency (WADA)</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100362"},"PeriodicalIF":4.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital identification and adulteration analysis of bupleurum chinense and bupleurum marginatum based on \"digital identity\" and UHPLC-QTOF-MSE","authors":"Xian rui Wang,&nbsp;Jia ting Zhang ,&nbsp;Wen guang Jing,&nbsp;Ming hua Li,&nbsp;Xiao han Guo,&nbsp;Xian long Cheng,&nbsp;Feng Wei","doi":"10.1016/j.talo.2024.100361","DOIUrl":"10.1016/j.talo.2024.100361","url":null,"abstract":"<div><h3>Background and objectives</h3><div>one of the legal sources of <em>Bupleuri Radix</em> (BR) is the <em>Bupleurum chinense</em> (BC), rather than the <em>Bupleurum marginatum</em> (BM). However, fake incidents of BM mixed with BC often occur in the market, which makes it more difficult to supervise the quality of BR and even endanger the life and health of patients. To strengthen the quality control and supervision of BP, we carried out digital identification and adulteration analysis of BC and BM based on the \"digital identity\" and UHPLC-QTOF-MS^E.</div></div><div><h3>Method</h3><div>firstly, the BC and BM were analyzed by UHPLC-QTOF-MS^E to obtain the quantized data characterization of chemical components. Secondly, the shared ions were extracted from different batches of BC and BM's control medicinal materials as their \"data representation of ions\", respectively. Then, the data matrices of unique ions of BC relative to BM and BM relative to BC were screened out, and the Top-N ions were outputted as the \"digital identities\" of BC and BM, sorted by ionic strength. Finally, the above \"digital identities\" of BC and BM were used as benchmarks for matching positive samples and commercial samples to feedback on matching credibility (MC).</div></div><div><h3>Results</h3><div>the results showed that based on the \"digital identities\" of BC and BM, the digital identification of BC, BM, and positive samples can be realized efficiently and accurately at the individual level of Chinese medicine, even if 3 % of BM in the mixed samples can still be identified efficiently and accurately. 10 batches of market samples were identified as adulterated samples. Furthermore, chemometric analysis has proven the reliability of BC and BM-based \"digital identity\" identification.</div></div><div><h3>Conclusion</h3><div>It proved that the identification and adulteration analysis of two herbs can be realized efficiently and quickly through the \"digital identities\" of BC and BM. It has important reference significance for developing non-targeted digital identification of herbal medicines at the individual level of Chinese medicine based on \"digital identity\", which was beneficial to the construction of digital Chinese medicine and digital quality control.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100361"},"PeriodicalIF":4.1,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecularly imprinted polymers-based biosensors for gynecological diagnostics and monitoring","authors":"Faezeh Ghorbanizamani ,&nbsp;Hichem Moulahoum ,&nbsp;Figen Zihnioglu ,&nbsp;Suna Timur","doi":"10.1016/j.talo.2024.100364","DOIUrl":"10.1016/j.talo.2024.100364","url":null,"abstract":"<div><div>Molecularly Imprinted Polymers (MIPs) offer promising advancements in gynecological diagnostics due to their high selectivity, stability, and cost-effectiveness. This review explores the application of MIP-based biosensors in detecting biomarkers for gynecological cancer, infections, and hormonal monitoring. Despite significant progress in MIP technology, its integration into clinical gynecology remains limited. The review provides a deep dive into the synthesis and characterization process of MIPs, current diagnostic methods, and the potential of emerging diagnostic approaches such as microfluidics and nanotechnology. Then, an overview of the various conditions, diseases, and potential biomarkers is explored. Emphasizing the importance of women's health, the review analyzes the latest research in MIP-based biosensing of gynecological conditions and calls for increased research and development to bridge the gap between laboratory innovation and clinical application. The goal is to enhance early detection, improve patient outcomes, and reduce healthcare costs. This advancement is essential for better disease management and personalized treatment in gynecology.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100364"},"PeriodicalIF":4.1,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in e-nose for potential applications in Covid-19 infection","authors":"Jay Rana,&nbsp;Sonal Desai","doi":"10.1016/j.talo.2024.100363","DOIUrl":"10.1016/j.talo.2024.100363","url":null,"abstract":"<div><div>E-nose devices are designed to detect odours and flavours. The versatility and sensitivity of e-nose technology make it a valuable tool in various fields including medical diagnosis, environmental monitoring, and security and safety. The utility of e-nose is extended to detect volatile organic compounds (VOCs) in breath and other bodily fluids that are indicative of COVID-19 infection. Several studies have shown promising results in terms of accuracy and sensitivity of e-nose devices for COVID-19 detection, and this technology has the potential to be used as a non-invasive and rapid screening tool for COVID-19 in high-risk environments such as hospitals and airports. However, further research and development are needed to optimize the sensitivity and specificity of e-nose devices for COVID-19 detection, and large-scale studies are needed to confirm the accuracy and effectiveness of these devices in real-world settings. This article covers the details about the purpose, working principle of e-nose along with its applicability for COVID-19 detection.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100363"},"PeriodicalIF":4.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of marijuana using silver-phosphine ion complexation and a semi-quantitative 1 % decision-point assay","authors":"Alleigh N. Couch ,&nbsp;Jayleigh M. Lanza ,&nbsp;Christopher M. Zall ,&nbsp;J. Tyler Davidson","doi":"10.1016/j.talo.2024.100359","DOIUrl":"10.1016/j.talo.2024.100359","url":null,"abstract":"<div><div>Due to the strict federal regulations concerning ∆⁹-tetrahydrocannabinol (∆⁹-THC) content for the differentiation of hemp and marijuana outlined in the 2018 Farm Bill, the ability to quickly and reliably differentiate cannabis as marijuana or hemp is crucial within both the seized drug community and hemp industry. This study provides a novel direct mass spectrometry approach for the identification of marijuana using Ag-phosphine ion complexation and a semi-quantitative 1 % decision-point assay. The main constituents of hemp and marijuana, cannabidiol (CBD) and ∆⁹-THC, are isomeric and cannot be differentiated using soft ionization mass spectrometry techniques alone. However, the incorporation of [Ag(PPh₃)(OTf)]₂ enables the formation of unique MS/MS product ions at <em>m/z</em> 421/423, <em>m/z</em> 353/355, and <em>m/z</em> 231 for CBD due to differences in binding affinity, allowing CBD to be differentiated from ∆⁹-THC. Likewise, the isomeric cannabinoid precursors ∆⁹-tetrahydrocannabinolic acid (∆⁹-THCA) and cannabidiolic acid (CBDA) can be differentiated due to the formation of unique MS/MS product ions at <em>m/z</em> 465/467 and <em>m/z</em> 379/381, which are specific to CBDA. Eight additional cannabinoids were also characterized utilizing the proposed Ag-phosphine ion complexation approach. To reduce the potential for false positives, a more conservative 1 % decision-point assay was developed by fortifying 1 % weight-by-volume ∆⁹-THC-<em>d₉</em> into methanolic extracts of authentic cannabis plant material, followed by assessing if the total ∆⁹-THC content (composed of ∆⁹-THC and ∆⁹-THCA) was greater than or less than the intensity of the spiked internal standard. When the developed approach was applied to 20 methanolic extracts of authentic cannabis samples with known cannabinoid compositions, 90 % of the samples were correctly identified as marijuana or not marijuana based on the 1 % administrative threshold for the total ∆⁹-THC content. The two lone misidentifications were due to the presence of elevated ∆⁸-THC, which highlights the necessity to explore more selective ligands in the future. This study provides the first application of Ag-ligand ion complexation for the identification of marijuana based on a semi-quantitative 1 % decision-point assay, which shows great promise as an alternative method for the rapid identification of marijuana.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100359"},"PeriodicalIF":4.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel, simple and rapid assay to measure citrate level in bacterial culture for analysis of citrate consumption by bacteria","authors":"Rattiyaporn Kanlaya,&nbsp;Chonnicha Subkod,&nbsp;Visith Thongboonkerd","doi":"10.1016/j.talo.2024.100360","DOIUrl":"10.1016/j.talo.2024.100360","url":null,"abstract":"<div><div>Citrate is essential for Krebs cycle in eukaryotes and serves as a carbon source for some bacteria to survive/grow. Available methods for measuring citrate level rely mainly on enzymatic reactions and/or sophisticated instrumentations. This study aimed to develop a novel, simple and rapid assay to quantify citrate in bacterial culture for analysis of citrate consumption. The assay was initially tested with 0.1–25.6 mM citrate in deionized (dI) water or complete M9 medium without/with 0.25 M HCl. Wavelength scan revealed maximum absorption of citrate at λ210 nm with linear calibration curve (R² = 0.9997–0.9999) when HCl was added. Among negatively charged chemicals tested, only oxalate interfered with the assay. After 24-h inoculation of <em>Klebsiella pneumoniae</em> (the known citrate-utilizing bacterium) in (20 mM) citrate-containing complete M9 medium, the remaining citrate significantly decreased (by 22.20±7.13 % consumption). However, a mild decrease was also observed in the sample without bacterial inoculation, suggesting that some components of the complete medium interfered with the assay. It was clearly evidenced that M9 salt, CaCl₂ and/or MgSO₄ were responsible for such interference. Finally, citrate at low concentrations (0.1–6.4 mM) in M9 medium with CaCl₂ and/or MgSO₄ provided the linear calibration curve (R² = 0.9451–0.9986). At 5 mM, citrate consumption by <em>K. pneumoniae</em> after 24-h culture was 46.88±0.47 %. In summary, we have successfully developed a novel, simple and rapid method for measuring citrate level in bacterial culture. It will be very useful for measuring citrate consumption by typical and atypical citrate-utilizing bacteria for classification, mechanistic and pathophysiologic studies.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100360"},"PeriodicalIF":4.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}