Talanta Open最新文献_第6页

3D-printed carbon black-based electrode for simultaneous determination of ascorbic acid and kojic acid in dermo-cosmetic products 同时测定皮肤化妆品中抗坏血酸和曲酸的3d打印炭黑基电极

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-08-20 DOI: 10.1016/j.talo.2025.100532

Larissa Gil Lucas , Eduardo Constante Martins , Pãmyla Layene dos Santos , João Paulo Winiarski , Edson Roberto Santana , Iolanda Cruz Vieira , Almir Spinelli

A miniaturized and disposable poly(lactic acid)-carbon black-based working electrode (3D CB-PLA) was developed using 3D printing technology through fused filament fabrication to be applied to the simultaneous analysis of the antioxidant compounds ascorbic acid (vitamin C) and kojic acid. A previous chemical/electrochemical activation of the 3D CB-PLA electrode was carried out in 1.0 mol L⁻¹ NaOH, applying a fixed potential of +1.0 V vs. Ag/AgCl, KCl_(sat) for 1800 s. This procedure was necessary to remove a smooth layer of PLA polymer on the electrode surface and to allow the carbon black conductive sites to be exposed. Morphological and spectroscopic characterizations performed before and after activation revealed a rougher surface with an increased presence of oxygenated functional groups, attributed to both the activation process and the enhanced exposure of carbon black. Using cyclic voltammetry in 0.1 mol L⁻¹ Britton-Robinson buffer (pH 7.0), the irreversible oxidation reactions of ascorbic and kojic acids were observed at +0.30 and +0.87 V vs. Ag/AgCl, KCl_(sat), respectively. With the experimental conditions optimized, calibration curves were constructed for both analytes by square wave voltammetry, with detection limits of 4.80 and 5.40 µmol L⁻¹ for ascorbic acid and kojic acid, respectively. The activated 3D CB-PLA electrode enabled accurate and precise simultaneous quantification of both antioxidant compounds in facial whitening creams, demonstrating its effectiveness and analytical potential.

利用3D打印技术，通过熔丝制作技术，研制了一种小型化的一次性聚乳酸-炭黑工作电极（3D CB-PLA），用于抗氧化剂抗坏血酸（维生素C）和曲酸的同时分析。在1.0 mol L−1 NaOH溶液中，施加+1.0 V vs. Ag/AgCl, KCl（sat）固定电位1800 s，对3D CB-PLA电极进行化学/电化学活化。这一过程是必要的，以去除电极表面的光滑层PLA聚合物，并允许炭黑导电部位暴露出来。在活化前后进行的形态学和光谱表征显示，由于活化过程和炭黑暴露增强，氧化官能团的存在增加，表面更粗糙。采用循环伏安法，在0.1 mol L−1 briton - robinson缓冲液（pH 7.0）中，在+0.30和+0.87 V （Ag/AgCl, KCl）下，观察了抗坏血酸和曲酸的不可逆氧化反应。在优化实验条件的基础上，建立了抗坏血酸和曲酸的方波伏安法校准曲线，检测限分别为4.80和5.40µmol L−1。激活的3D CB-PLA电极能够准确和精确地同时定量面部美白霜中的两种抗氧化化合物，证明了其有效性和分析潜力。

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引用次数: 0

High-throughput microfluidic generation of self-assembled, uniform 3D vascularized and mineralized bone organoids without matrix biomaterials 无基质生物材料的自组装、均匀三维血管化和矿化骨类器官的高通量微流控生成

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-08-15 DOI: 10.1016/j.talo.2025.100531

Xue Li , Zijiao Zhang , Tian Tian , Chen Chen , Xin Xu , Yuzhu He , Yaran Zang , Jianan Hui , Hongju Mao , Huiying Liu

Bone organoids hold great promise for modeling bone-related diseases, improving bone injury repair strategies, and enabling high-throughput drug screening. However, conventional approaches rely heavily on matrix biomaterials—such as Matrigel, collagen gels, or 3D-printed scaffolds—which introduce undefined parameters, pose manufacturing complexities, and raise cost barriers, potentially limiting clinical translation. To address these challenges, we present a novel high-throughput microfluidic chip that generates 540 uniformly sized, scaffold-free 3D bone organoids simultaneously within six independent channels. By co-cultivating human bone marrow mesenchymal stem cells (hBMSCs), human umbilical vein endothelial cells (HUVECs), and mineralized collagen (MC), self-assembled bone organoids formed by the third day and progressively compacted over time, exhibiting enhanced cell viability and proliferation. The inclusion of MC upregulated multiple osteogenic markers (OCN, ALP, COL-1, RUNX2, and BMP-2), while endothelial markers (PECAM-1, HIF-1α, and VEGF) remained consistently expressed, reflecting stable vascularization and mineralization potential. Overall, this high-throughput, matrix-free microfluidic platform offers a biomimetic environment for the investigation of osteogenesis and angiogenesis and holds significant promise for advanced material assessment, drug screening, and disease modeling.

骨类器官在骨相关疾病建模、改善骨损伤修复策略和实现高通量药物筛选方面具有很大的前景。然而，传统的方法严重依赖于基质生物材料，如Matrigel、胶原蛋白凝胶或3d打印支架，这些材料引入了未定义的参数，造成了制造的复杂性，并提高了成本壁垒，潜在地限制了临床转化。为了解决这些挑战，我们提出了一种新型的高通量微流控芯片，它可以在六个独立的通道内同时生成540个均匀大小的无支架3D骨类器官。通过共同培养人骨髓间充质干细胞（hBMSCs）、人脐静脉内皮细胞（HUVECs）和矿化胶原（MC），自组装的类骨器官在第三天形成，并随着时间的推移逐渐压缩，显示出增强的细胞活力和增殖能力。MC的加入上调了多种成骨标志物（OCN、ALP、COL-1、RUNX2和BMP-2），而内皮标志物（PECAM-1、HIF-1α和VEGF）的表达保持一致，反映了稳定的血管化和矿化潜力。总的来说，这种高通量、无基质的微流控平台为研究成骨和血管生成提供了一个仿生环境，并在先进的材料评估、药物筛选和疾病建模方面具有重要的前景。

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引用次数: 0

Nanozyme activity of silver nanowires for colorimetric detection of bisphenol A following salting-out assisted liquid-liquid extraction 盐析辅助液-液萃取比色法检测双酚A的银纳米线纳米酶活性研究

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-10-30 DOI: 10.1016/j.talo.2025.100587

Huda Salem AlSalem , Sara Naif Alharbi , Mohamed A Abdel-Lateef , Rabeea D. Abdel-Rahim , Wesam M. El-Koussi

The increasing use of industrial chemicals has raised major concerns about their environmental and health impacts, with bisphenol A (BPA) being one of the most widely studied contaminants. In this work, we introduce a proof-of-concept approach that combines the peroxidase-like catalytic activity of silver nanowires (AgNWs) with a salting-out assisted liquid–liquid extraction (SALLE) strategy for BPA detection. AgNWs catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in an acidic environment, producing a measurable color change that is effectively suppressed in the presence of BPA. This inhibition enables a simple colorimetric quantification of BPA with a detection limit of 31.62 ng/mL (LOQ = 95.83 ng/mL). The method demonstrated a practical linear response within the range of 200–700 ng/mL, making it suitable for screening moderately contaminated water samples. Furthermore, the work offers good recovery values for the extraction of BPA from spiked environmental water samples. Integrating AgNWs nanoenzyme activity with an eco-friendly pre-concentration step provides a promising low-cost, straightforward, and rapid alternative to conventional chromatographic methods, particularly suited for preliminary screening of contaminated waters.

越来越多的工业化学品的使用引起了人们对其环境和健康影响的关注，双酚A （BPA）是研究最广泛的污染物之一。在这项工作中，我们介绍了一种概念验证方法，该方法将银纳米线（AgNWs）的过氧化物酶样催化活性与盐析辅助液液萃取（SALLE）策略相结合，用于BPA检测。AgNWs在酸性环境中催化3,3 ',5,5 ' -四甲基联苯胺（TMB）的氧化，产生可测量的颜色变化，这种变化在BPA存在下被有效抑制。该抑制作用可实现双酚a的简单比色定量，检出限为31.62 ng/mL （LOQ = 95.83 ng/mL）。该方法在200 ~ 700 ng/mL范围内具有良好的线性响应，适用于中度污染水样的筛选。此外，该工作为从加标环境水样中提取BPA提供了良好的回收率值。将AgNWs纳米酶活性与生态友好的预浓缩步骤相结合，为传统色谱方法提供了一种低成本、简单、快速的替代方法，特别适合于污染水的初步筛选。

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引用次数: 0

Graphene oxide and molecularly imprinted polymer-based sensor for the electrochemical detection of benzene: A novel tactic in early diagnosis of lung cancer 基于氧化石墨烯和分子印迹聚合物的苯电化学检测传感器：肺癌早期诊断的新策略

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-06-25 DOI: 10.1016/j.talo.2025.100506

Warren Rosario , Devesh K. Avasthi , Nidhi Chauhan

Early cancer detection significantly enhances survival rates, making widespread diagnostic screening essential. However, the high cost and limited accessibility of diagnostic tools pose major challenges. Nanomaterial-based sensors offer a promising solution due to their portability, user-friendly design, and cost-effective manufacturing. Volatile organic compounds (VOCs) emitted by the human body are closely linked to the presence of life-threatening diseases, with benzene emerging as a significant biomarker in the breath and blood of individuals at risk of developing lung cancer. Leveraging this correlation, we have developed a sensor capable of detecting benzene with high reliability. This study details the fabrication of an electrochemical sensor on a screen-printed electrode (SPE), modified with graphene oxide (GO) followed by molecularly imprinted polymer (MIP). SPE promotes the development of a miniaturized and user-friendly sensing platform. The incorporation of GO is responsible for improving the sensor's response due to its excellent electrochemical properties. The MIP is synthesized using benzene as a template, which creates imprints of benzene on the polymer. Integration of this MIP significantly improves the sensor’s selectivity. This approach addresses the key limitation of low selectivity in VOC sensors for medical applications. The sensor demonstrated a broad linear range of 0.1–1000 ppb with a sensitivity of 15.5 µA/ppb and exhibited excellent selectivity toward benzene. By enhancing both sensitivity and selectivity, the developed sensor offers a promising solution toward effective, accessible, and early lung cancer diagnosis.

早期癌症检测可显著提高生存率，因此广泛的诊断筛查必不可少。然而，诊断工具的高成本和有限的可及性构成了主要挑战。基于纳米材料的传感器由于其便携性、用户友好的设计和具有成本效益的制造而提供了一个有前途的解决方案。人体排放的挥发性有机化合物（VOCs）与危及生命的疾病密切相关，苯成为有患肺癌风险个体呼吸和血液中的重要生物标志物。利用这种相关性，我们开发了一种能够高可靠性检测苯的传感器。本研究详细介绍了在丝网印刷电极（SPE）上制造电化学传感器的过程，该电极用氧化石墨烯（GO）和分子印迹聚合物（MIP）修饰。SPE促进了小型化和用户友好型传感平台的发展。氧化石墨烯的加入是负责改善传感器的响应，由于其优异的电化学性能。MIP是用苯作为模板合成的，它在聚合物上产生苯的印迹。该MIP的集成显著提高了传感器的选择性。这种方法解决了用于医疗应用的VOC传感器低选择性的关键限制。该传感器具有0.1-1000 ppb的宽线性范围，灵敏度为15.5µa /ppb，对苯具有优异的选择性。通过提高灵敏度和选择性，所开发的传感器为有效、方便和早期肺癌诊断提供了一个有希望的解决方案。

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引用次数: 0

A review on biosensor approaches for the detection of hazardous elements in water 生物传感器在水中有害元素检测中的研究进展

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-08-23 DOI: 10.1016/j.talo.2025.100536

Nidhi Chauhan , Nishtha Sah , Kirti Saxena , Utkarsh Jain

Water is the basis of life on Earth, but due to increased pollution and habitat degradation, water scarcity is increasing day by day. Therefore, it is necessary to detect water pollutants to save water and aquatic diversity. Monitoring of toxic elements present in wastewater effluents is an important factor for further treatment to make it pollutant-free. While various methods have been developed for monitoring these toxic elements, biosensors can overcome the limitations of traditional methods. Biosensors are highly sensitive, selective, low in cost, and simple to use. In this review, we have provided an overview of reported biosensors for the detection of various types of toxic elements that cause water toxicity. A summary of these toxic elements has also been discussed herein. This review aims to provide an overview of advances in biosensing technology used for water monitoring.

水是地球上生命的基础，但由于污染加剧和栖息地退化，水资源短缺日益严重。因此，有必要检测水污染物，以节约用水和水生多样性。监测废水中存在的有毒元素是进一步处理以使其无公害的重要因素。虽然已经开发了各种方法来监测这些有毒元素，但生物传感器可以克服传统方法的局限性。生物传感器具有灵敏度高、选择性强、成本低、使用简单等特点。在这篇综述中，我们提供了报道的生物传感器检测引起水毒性的各种有毒元素的概述。本文还对这些有毒元素作了概述。本文综述了生物传感技术在水监测中的应用进展。

引用次数: 0

Wearable biosensors in modern healthcare: Emerging trends and practical applications 现代医疗保健中的可穿戴生物传感器：新兴趋势和实际应用

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-05-30 DOI: 10.1016/j.talo.2025.100486

Maumita Das Mukherjee , Priyanshi Gupta , Vanshika Kumari , Ishika Rana , Drishti Jindal , Navya Sagar , Jay Singh , Chetna Dhand

Wearable biosensor systems represent a significant breakthrough in the life sciences, offering real-time monitoring and quantitative assessment of various human health parameters. The escalating demand for continuous and immediate surveillance of both acute and chronic conditions, compounded by constraints in clinical infrastructure, has propelled extensive investigation into wearable biosensors. These systems have emerged as pivotal tools for furnishing physiological insights and facilitating non-invasive monitoring. They monitor various analytes through the analysis of sweat, epidermal, Interstitial fluids, breath and tears, and easily incorporated into flexible substrates ensuring long-term wear and continuous monitoring. However, these biosensors penetrate deeper into the body's fluid networks, providing a systemic approach to continuous monitoring of biomarkers, which is particularly useful for individuals with metabolic disorders. This comprehensive review explores recent trends and diverse applications of wearable biosensors. These applications empower individuals to dynamically assess their well-being, enabling informed healthcare decisions tailored to individual needs. The convergence of these approaches in wearable biosensors highlights a comprehensive approach to understand individual health. This integration holds the promise of transforming healthcare practice by providing continuous observance and remote monitoring. The result has been a paradigm shift towards active, personalized health care, leading to improved health outcomes and improved quality of life

可穿戴生物传感器系统代表了生命科学的重大突破，提供了各种人体健康参数的实时监测和定量评估。对急性和慢性疾病持续和即时监测的需求不断增加，加上临床基础设施的限制，推动了对可穿戴生物传感器的广泛研究。这些系统已成为提供生理学见解和促进非侵入性监测的关键工具。它们通过分析汗液，表皮，间质液，呼吸和眼泪来监测各种分析物，并且很容易结合到柔性基板中，确保长期磨损和连续监测。然而，这些生物传感器深入人体的流体网络，提供了一种系统的方法来连续监测生物标志物，这对代谢紊乱的个体特别有用。这篇综合综述探讨了可穿戴生物传感器的最新趋势和各种应用。这些应用程序使个人能够动态评估自己的健康状况，从而根据个人需求做出明智的医疗保健决策。这些方法在可穿戴生物传感器中的融合突出了了解个人健康的综合方法。这种集成有望通过提供持续观察和远程监控来改变医疗保健实践。其结果是向积极、个性化的医疗保健模式转变，从而改善了健康结果，提高了生活质量

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引用次数: 0

Chemical imprinting meets nanotechnology: Ultra-sensitive monitoring of beta-endorphin 化学印迹与纳米技术相结合：超灵敏的内啡肽监测

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-08-12 DOI: 10.1016/j.talo.2025.100529

Ankita Ghosh , Ramesh Chandra , Nidhi Chauhan

Beta-endorphin (BE) is an endogenous opioid peptide (EOP) produced in the anterior pituitary gland and hypothalamus. Beta-endorphin is a vital neurotransmitter involved in physiological processes including relief the pain, boosting memory, and regulating mood. BE is a happy hormone that reduces the chance of depression and anxiety. BE analysed by traditional laboratory techniques like LC-MS, ELISA and radioimmunoassay is reliable; however, there are certain limitations including time consumption, device complexity, cost and availability. This study aims to develop a self-monitoring molecularly imprinted polymer-based (MIP) biosensor for sensitive, selective and rapid detection of BE. The nanocomposite TiO₂/MoS₂ was electrochemically deposited on the screen-printed electrode using cyclic voltammetry (CV) to enhance the conductivity and surface area to immobilize MIP. The MIP was electrochemically deposited at voltage range 0.2 to -0.6 V using CV for 20 cycles at scan rate 50 mV/s on the modified TiO₂/MoS₂/SPE. MIP is a synthetic bio-recognizing element that provides high selectivity for BE over other structurally similar molecules. The developed sensor MIP@TiO₂/MoS₂/SPE exhibits sensitivity of 0.475 µA/pM, the limit of detection (LOD) of 0.1pM and a detection range of 0.1pM and 200pM. This is novel, innovative and cost-effective point of care device developed for the rapid and real time detection of BE.

内啡肽（BE）是一种内源性的阿片肽（EOP），产生于脑垂体前叶和下丘脑。-内啡肽是一种重要的神经递质，参与缓解疼痛、提高记忆力和调节情绪等生理过程。BE是一种快乐激素，可以减少抑郁和焦虑的机会。用LC-MS、ELISA和放射免疫分析法等传统实验室技术分析BE是可靠的；然而，有一些限制，包括时间消耗、设备复杂性、成本和可用性。本研究旨在开发一种基于分子印迹聚合物（MIP）的自监测生物传感器，用于灵敏、选择性和快速检测BE。利用循环伏安法（CV）将纳米复合材料TiO2/MoS2电化学沉积在丝网印刷电极上，以提高电导率和表面积，固定MIP。MIP在修饰后的TiO2/MoS2/SPE上，以50 mV/s的扫描速率，在0.2 ~ -0.6 V的电压范围内，使用CV进行20个循环的电化学沉积。MIP是一种合成的生物识别元件，它比其他结构相似的分子提供高选择性的BE。该传感器MIP@TiO2/MoS2/SPE灵敏度为0.475µA/pM，检出限（LOD）为0.1pM，检测范围为0.1pM和200pM。这是一种新颖，创新和具有成本效益的护理点设备，用于快速和实时检测BE。

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引用次数: 0

Electrochemical detection of enrofloxacin and ciprofloxacin based on mesoporous La2O2CO3 nanostructures – an eco-friendly electrode material for sustainability 基于介孔La2O2CO3纳米结构的可持续性环保电极材料电化学检测恩诺沙星和环丙沙星

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-09-03 DOI: 10.1016/j.talo.2025.100546

Arghya Chakravorty , Sudip Das , Sahil Luktuke , Aarcha Appu Mini , Vimala Raghavan

Administration of antibiotics in the modern world has posed a serious threat to induce antimicrobial resistance (AMR), which concerns the Sustainable Development Goals (SDGs). Fluoroquinolone group antibiotics, namely enrofloxacin and ciprofloxacin, are predominantly responsible for undermining the One Health. In this context, to provide an eco-friendly and robust approach, a green electrochemical sensor has been fabricated with lanthanum oxycarbonate (La₂O₂CO₃) nanoparticles for the detection of enrofloxacin and ciprofloxacin in several complex matrices, related to food safety, environmental monitoring, and human health. The green synthesis methodology was adopted for the synthesis of the oxycarbonate material, while the phytochemicals present in the leaves of Sida acuta played the role of reducing and capping agents. To characterize the crystallinity and morphology of the synthesized material, several spectroscopic (XRD, BET, EDAX) and microscopic (FESEM, TEM) techniques were employed. The fabricated electrodes were characterized by atomic force microscopy (AFM), goniometry, and surface profilometry. The fabricated voltammetric sensor demonstrated an efficient linear range of 2.77 µM to 158.99 µM and 5.98 µM to 57.87 µM in the case of enrofloxacin and ciprofloxacin, respectively. In both cases, cyclic voltammetry (CV), differential pulse voltammetry (DPV), and square wave voltammetry (SWV) have been employed as electrochemical analytical techniques. The limit of detection (LOD) was achieved to be 4.07 µM, 2.91 µM, and 4.98 µM, while the limit of quantification (LOQ) was achieved to be 14.2 µM, 8.83 µM, and 15.1 µM in CV, DPV, and SWV, respectively, for the detection of enrofloxacin. On the other hand, the LOD was achieved to be 2.85 µM, 1.90 µM, and 1.65 µM, while the LOQ was found as 8.64 µM, 5.7 µM, and 5.01 µM in CV, DPV, and SWV, respectively, for the detection of ciprofloxacin.

在现代世界，抗生素的使用对诱发抗菌素耐药性（AMR）构成了严重威胁，这关系到可持续发展目标（sdg）。氟喹诺酮类抗生素，即恩诺沙星和环丙沙星，是破坏“同一个健康”的主要原因。在此背景下，为了提供一种环保和可靠的方法，我们用氧化碳酸镧（La2O2CO3）纳米粒子制作了一种绿色电化学传感器，用于检测几种复杂基质中的恩诺沙星和环丙沙星，这些基质与食品安全、环境监测和人类健康有关。采用绿色合成方法合成碳酸氧材料，同时利用芦笋叶中的植物化学物质发挥还原剂和封盖剂的作用。为了表征合成材料的结晶度和形貌，采用了多种光谱（XRD， BET， EDAX）和微观（FESEM， TEM）技术。用原子力显微镜（AFM）、角形仪和表面轮廓仪对制备的电极进行了表征。所制备的伏安传感器在恩诺沙星和环丙沙星的有效线性范围分别为2.77µM ~ 158.99µM和5.98µM ~ 57.87µM。在这两种情况下，循环伏安法（CV）、差分脉冲伏安法（DPV）和方波伏安法（SWV）都被用作电化学分析技术。恩诺沙星的检出限（LOD）分别为4.07µM、2.91µM和4.98µM，定量限（LOQ）分别为14.2µM、8.83µM和15.1µM。另一方面，环丙沙星在CV、DPV和SWV中的LOQ分别为2.85µM、1.90µM和1.65µM， LOQ分别为8.64µM、5.7µM和5.01µM。

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引用次数: 0

Novel and Ultra-sensitive Harnessing of Native Fluorescence and Bio-Inspired N, S-CQDs: A Greener Path to Pharmaceutical and Biological Analysis of Moexipril with Enhanced Sustainability Profiles 新型超灵敏的天然荧光和生物激发N， S-CQDs：莫西普利药物和生物分析的绿色途径，具有增强的可持续性特征

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-07-11 DOI: 10.1016/j.talo.2025.100516

Neamat T. Barakat, Heba Abd El-Aziz, Manal I. Eid, Fawzia A. Ibrahim

Monitoring the level of Moexipril is crucial for hypertensive patients, therefore two environmentally friendly, rapid and selective analytical techniques have been established for its sensitive measurement. First method relies on direct measurement of its native fluorescence at λ_ex 282 nm / λ_em 315 nm. The second method is based on the efficient and quantitative enhancement of N, S-CQDs fluorescence intensity by Moexipril in the presence of 1% Tween. The remarkably fluorescent N and S-CQDs were developed via 8 minutes microwave-aided one-pot synthesis using promising, cheap and widely available precursors (Arugula leaves) for the first time. The sensor is characterized by excellent doping efficiency (N, 13.01% and S, 12.39%), narrow particle size distribution (2.7±0.6 nm), reproducibility and good fluorescence at λ_ex 320nm / λ_em 430 nm with 68.5% quantum yield. Also, the designed probe selectivity was verified in presence of possible co-existing excipients, various metal ions and co-administered drugs that grants the practical applicability of N, S-CQDs as an environmental probe. Additionally, both methods showed excellent linearity (r = 0.9999) within the specified concentrations range (0.02-0.6 and 0.004-0.7 µg/mL for method I and II, respectively) and they were implemented for the analysis of Moexipril in pharmaceutical tablets and biological fluids, yielding high recovery percentages. The proposed procedures were fully validated in compliance with the ICH Guidelines. Moreover, the greenness, blueness and whiteness profiles of developed methods were evaluated for accomplishment of a complete ecological profile of the designed fluorimetric methodologies and the excellent obtained results confirmed their excellent eco friendless, good applicability and sustainability.

莫艾昔普利的监测对高血压患者至关重要，因此建立了两种环境友好、快速、选择性的分析技术，对其进行灵敏的检测。第一种方法依赖于直接测量其在λex 282 nm / λem 315 nm的天然荧光。第二种方法是基于莫艾西普利在1%吐温存在下对N， S-CQDs荧光强度的有效和定量增强。首次以芝麻菜叶为原料，采用8分钟微波辅助一锅法合成了具有显著荧光的N和S-CQDs。该传感器具有良好的掺杂效率（N为13.01%，S为12.39%）、较窄的粒径分布（2.7±0.6 nm）、重复性好、在λex 320nm / λem 430 nm处具有良好的荧光性和68.5%的量子产率。此外，在可能共存的赋形剂、各种金属离子和共同给药的情况下，验证了所设计的探针的选择性，从而使N， S-CQDs作为环境探针具有实际的适用性。方法1和方法2在规定的浓度范围内（方法1为0.02 ~ 0.6µg/mL，方法2为0.004 ~ 0.7µg/mL）均具有良好的线性关系（r = 0.9999），可用于莫艾昔普利片剂和生物液中莫艾昔普利的分析，回收率高。拟议的程序已根据ICH指南进行了充分验证。此外，对所开发方法的绿度、蓝度和白度曲线进行了评价，以完成所设计的荧光法的完整生态剖面，所获得的优异结果证实了其良好的生态友好性、良好的适用性和可持续性。

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引用次数: 0

Automated MIP-based in-tip dispersive micro-solid phase extraction for determination of ketoprofen in environmental water: A proof of concept and performance evaluation 基于mip的自动化尖端分散微固相萃取法测定环境水中的酮洛芬：概念验证和性能评价

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-07-18 DOI: 10.1016/j.talo.2025.100522

Mohammed T. Shehabeldeen , Fotouh R. Mansour , Samah F. El-Malla , Aya A. Abdella

Automated in-tip dispersive micro-solid phase extraction is a miniaturized technique that could afford advantages of solid phase extraction with fewer steps and reduced environmental hazard. In this work, molecularly imprinted polymer was evaluated as a packing sorbent for a lab-made automated dispersive pipette tip extraction (MIP-DPX). The MIP was prepared using chitosan as the functional monomer, glutaraldehyde as the crosslinker, and ketoprofen (KTP) as the template molecule. The efficiency of KTP extraction using the devised automated tip was studied by investigating the effect of adsorption pH, elution volume, and the number of aspirating/dispensing cycles. High recovery rates were obtained at pH 4 employing 8 cycles of aspiration followed by KTP elution using methanol (500 μL) in a single dispensing cycle. The developed MIP-DPX demonstrated good linearity and reproducibility, making it suitable for KTP determination in environmental water. MIP-DPX was evaluated for environmental friendliness using the Complex Green Analytical Procedure Index (Complex GAPI) and Analytical GREEnness metric (AGREE), as well as practicality using the Blue Applicability Grade Index (BAGI). The developed workflow was proved to be a practical, environmentally friendly, and economic approach for pre-concentration and determination of KTP. The devised in-tip extraction system showed promising results paving the way for expanding the applicability of MIP-based SPE towards their automation.

自动化尖端分散微固相萃取是一种具有固相萃取步骤少、环境危害小等优点的小型化技术。在这项工作中，分子印迹聚合物被评估为实验室自制的自动分散移液管尖端提取（MIP-DPX）的填料吸附剂。以壳聚糖为功能单体，戊二醛为交联剂，酮洛芬（KTP）为模板分子制备了MIP。通过考察吸附pH值、洗脱量、吸气/分配循环次数等因素对KTP提取效率的影响，研究了该自动尖端的萃取效率。在pH为4的条件下，采用8次吸出，然后用500 μL的甲醇进行KTP洗脱，单次分配循环，回收率高。所建立的MIP-DPX具有良好的线性和重现性，适用于环境水体中KTP的测定。使用复杂绿色分析程序指数（Complex GAPI）和分析绿色度量（AGREE）评估MIP-DPX的环境友好性，以及使用蓝色适用性等级指数（BAGI）评估其实用性。实验证明，该流程是一种实用、环保、经济的KTP预富集测定方法。所设计的尖端萃取系统取得了良好的效果，为扩大基于mip的SPE在自动化方面的适用性铺平了道路。

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