Talanta Open最新文献_第9页

An analytical method to quantify tetrabromobisphenol A (TBBPA) and its conjugated metabolites in rat serum or other biological samples 一种定量大鼠血清或其它生物样品中四溴双酚A （TBBPA）及其共轭代谢物的分析方法

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-08-23 DOI: 10.1016/j.talo.2025.100537

Mette Stub, Mikael Pedersen, Louise Ramhøj, Kit Granby, Terje Svingen, Agnieszka Anna Niklas

Brominated flame retardants (BFRs) are widely used to enhance fire resistance in consumer and commercial products, but often leach into the environment due to their surface application. Tetrabromobisphenol A (TBBPA), the most applied BFR, has been detected in human serum, urine, and breast milk. TBBPA is readily absorbed from the gastrointestinal tract, undergoing first-pass hepatic metabolism to form the two major metabolites TBBPA-sulfate and TBBPA-glucuronide. Despite evidence linking TBBPA exposure to developmental toxicity, neurobehavioral effects, uterine cancers, and endocrine disrupting potential via thyroid hormone interference, exposure assessments remain challenging. Current methods rely heavily on direct quantification of TBBPA and its metabolites using liquid chromatography-mass spectrometry (LC-MS), but a lack of commercially available TBBPA metabolites at analytical standards is a limiting factor. This study introduces a method to rapidly quantify TBBPA, and after enzymatic deconjugation, its metabolites using LC-Orbitrap. Using this method, TBBPA-glucuronide was the only metabolite detected in rat serum following oral exposure. Native TBBPA was obtained using enzymatic deconjugation of serum. The protocol simplifies exposure evaluation by offering a practical, and rapid, approach for quantifying TBBPA in biological samples.

溴化阻燃剂（BFRs）广泛用于提高消费品和商业产品的耐火性，但由于其表面应用，往往会渗入环境中。四溴双酚A （TBBPA）是应用最广泛的BFR，已在人类血清、尿液和母乳中检测到。TBBPA很容易被胃肠道吸收，经过肝脏首过代谢形成两种主要代谢物TBBPA-sulfate和TBBPA-glucuronide。尽管有证据表明TBBPA暴露与发育毒性、神经行为效应、子宫癌和通过甲状腺激素干扰内分泌的潜在干扰有关，但暴露评估仍然具有挑战性。目前的方法严重依赖于使用液相色谱-质谱（LC-MS）直接定量TBBPA及其代谢物，但缺乏市售的分析标准TBBPA代谢物是一个限制因素。本研究介绍了一种快速定量TBBPA的方法，并在酶解后使用LC-Orbitrap测定其代谢产物。采用该方法，tbbpa -葡糖苷是口服暴露后大鼠血清中唯一检测到的代谢物。用酶解血清获得天然TBBPA。该方案通过提供一种实用、快速的方法来量化生物样品中的TBBPA，从而简化了暴露评估。

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引用次数: 0

Analytical methodologies for antioxidant capacity assessment: An updated review 抗氧化能力评估的分析方法：最新综述

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-09-14 DOI: 10.1016/j.talo.2025.100558

Mahmoud Hamed , Faisal K. Algethami , Alaa Bedair , Fotouh R. Mansour

Antioxidants are crucial molecules protecting biological systems from harmful oxidation reactions and free radicals. This class of compounds attracted remarkable attention for their potential in functional foods and dietary supplements. The imbalance between oxidants and antioxidants leads to oxidative stress, associated with various chronic diseases. Accurate measurement of antioxidant activity is therefore essential for evaluating potential health-enhancing agents in food science, medicine, and biotechnology. This review provides a comprehensive overview of contemporary analytical methodologies for assessing antioxidant capacity across food, cosmetic, pharmaceutical, and biomedical applications, exploring four major categories of techniques: spectrophotometric methods, including UV–Vis, electron paramagnetic resonance (EPR), and near infrared (NIR) spectroscopy, highlighting their principles and applications in radical scavenging assays and antioxidant characterization. Electrochemical techniques are rapid, sensitive, and cost-effective alternatives, focusing on voltammetry, amperometry, and biosensor development for direct measurement of electron transfer processes. The role of biosensors, employing bioreceptors like enzymes and DNA, for selective and sensitive antioxidant detection is also examined. Chromatographic methods, such as high-performance liquid chromatography (HPLC) and gas chromatography (GC), paired with selective detectors, are used to separate, identify, and quantify antioxidant compounds in complex matrices. This review highlights the principles, advancements, advantages, and limitations of these diverse methodologies in the accurate evaluation of antioxidant potential.

抗氧化剂是保护生物系统免受有害氧化反应和自由基侵害的关键分子。这类化合物因其在功能性食品和膳食补充剂中的潜力而备受关注。氧化剂和抗氧化剂之间的不平衡导致氧化应激，与各种慢性疾病有关。因此，准确测量抗氧化活性对于评估食品科学、医学和生物技术中潜在的健康促进剂至关重要。本文综述了食品、化妆品、制药和生物医学领域抗氧化能力评估的现代分析方法，探讨了四大类技术：分光光度法，包括紫外-可见、电子顺磁共振（EPR）和近红外（NIR）光谱，重点介绍了它们在自由基清除试验和抗氧化表征中的原理和应用。电化学技术是一种快速、灵敏、经济的替代方法，主要集中在伏安法、安培法和生物传感器的开发上，用于直接测量电子转移过程。生物传感器的作用，采用生物受体如酶和DNA，选择性和敏感的抗氧化剂检测也进行了检查。色谱方法，如高效液相色谱（HPLC）和气相色谱（GC），与选择性检测器配对，用于分离、鉴定和定量复杂基质中的抗氧化化合物。本文综述了这些不同方法在准确评价抗氧化潜力方面的原理、进展、优势和局限性。

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引用次数: 0

A simple and high-throughput ICP-MS method for the determination of 46 elements in urine: Biomonitoring of occupational exposure in automotive workers 一种简单、高通量ICP-MS测定尿液中46种元素的方法：汽车工人职业暴露的生物监测

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-09-14 DOI: 10.1016/j.talo.2025.100559

Ruirui Dai , Huadong Zhang , Wei Yang , Xiaoxi Wang , Xiaoping Li , Yujie Yu , Fengqiong Chen

Monitoring urinary metal concentrations is crucial for assessing occupational exposure and associated health risks. Inductively coupled plasma mass spectrometry (ICP-MS) is widely considered the method of choice for multi-element analysis due to its high sensitivity, low detection limits, and broad dynamic range. This study presents a simple and rapid ICP-MS method for the simultaneous determination of 46 elements in urine, utilizing 2 % nitric acid and 1.5 % ethanol (v/v) for sample pretreatment. This approach effectively mitigates matrix effects and carbon-based interferences. The method demonstrated excellent linearity (coefficient of determination, R² ≥ 0.9985), precision (relative standard deviation, RSD < 8 %), and accuracy (recoveries 80–120 %). The method was applied to analyze 268 urine samples from automotive manufacturing workers. Several elements of occupational relevance, including Nickel (Ni), tungsten (W), molybdenum (Mo), titanium (Ti) and Cadmium (Cd), as well as essential elements such as iron (Fe), copper (Cu), and zinc (Zn), were elevated compared with general population references, indicating complex multi-metal exposure profiles. This work provides a validated and efficient tool for occupational biomonitoring and delivers valuable elementomic data to support exposure assessment, workplace interventions, and future research on the health effects of multi-metal exposure.

监测尿中金属浓度对于评估职业接触和相关健康风险至关重要。电感耦合等离子体质谱法（ICP-MS）因其灵敏度高、检出限低、动态范围宽等优点被广泛认为是多元素分析的首选方法。采用2%硝酸和1.5%乙醇（v/v）进行样品预处理，建立了简便、快速的ICP-MS同时测定尿液中46种元素的方法。这种方法有效地减轻了基体效应和碳基干扰。该方法具有良好的线性（决定系数，R²≥0.9985）、精密度（相对标准偏差，RSD < 8%）和准确度（加样回收率80 ~ 120%）。该方法应用于268份汽车制造工人的尿样分析。与一般人群相比，与职业相关的几种元素，包括镍（Ni）、钨(W)、钼（Mo）、钛（Ti）和镉（Cd），以及必需元素，如铁（Fe）、铜（Cu）和锌（Zn），都有所升高，表明复杂的多金属暴露谱。这项工作为职业生物监测提供了一个有效的工具，并提供了有价值的元素数据，以支持暴露评估、工作场所干预和未来多金属暴露对健康影响的研究。

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引用次数: 0

A ratiometric NIR fluorescent probe for simultaneous detection of H2S and viscosity: Applications in environmental monitoring and bioimaging 同时检测H2S和黏度的比例近红外荧光探针：在环境监测和生物成像中的应用

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-09-23 DOI: 10.1016/j.talo.2025.100564

Sha Li, Qin Li, Zhihui Yang, Wei Wen, Miao Yan, Haixian Ren

Hydrogen sulfide (H₂S), a critical gasotransmitter governing physiological functions, presents significant environmental and food safety hazards at aberrant concentrations. Concurrently, mitochondrial viscosity serves as a vital biomarker for cellular pathologies. To address the unmet need for precise dual-parameter monitoring, we engineered a mitochondria-targeted near-infrared (NIR) ratiometric fluorescent probe, T-N, enabling simultaneous and discriminative detection of H₂S and viscosity through discrete emission channels. The probe exhibits ultrarapid response (<30 s), exceptional sensitivity, and superb selectivity against competing analytes. Leveraging H₂S-triggered chromogenic shifts, T-N-immobilized test strips facilitate instrument-free, semi-quantitative assessment of food spoilage, validated across environmental waters and industrially relevant beer samples. Moreover, T-N achieves synchronous dual-channel imaging of endogenous/exogenous H₂S and viscosity dynamics in live cells, with verified mitochondrial localization. This work establishes a versatile sensing platform for tracking H₂S and viscosity in interconnected environmental, food safety, and biomedical contexts.

硫化氢（H2S）是一种控制生理功能的重要气体递质，在异常浓度下会对环境和食品安全造成重大危害。同时，线粒体粘度作为细胞病理的重要生物标志物。为了满足精确双参数监测的需求，我们设计了一种针对线粒体的近红外（NIR）比例荧光探针T-N，可以通过离散发射通道同时鉴别检测H2S和粘度。该探针具有超快速响应（30秒），卓越的灵敏度和对竞争分析物的极好选择性。利用h2s触发的显色变化，t- n固定试纸可以实现无仪器、半定量的食品腐败评估，并在环境水域和工业相关的啤酒样品中得到验证。此外，T-N在活细胞中实现了内源性/外源性H2S和黏度动力学的同步双通道成像，并验证了线粒体定位。这项工作建立了一个多功能传感平台，用于在相互关联的环境、食品安全和生物医学环境中跟踪H2S和粘度。

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引用次数: 0

High-performance MIL-53(Fe)-incorporated cellulose acetate membranes for efficient dye and wastewater treatment 高性能MIL-53(Fe)-醋酸纤维素膜，用于高效染料和废水处理

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-07-14 DOI: 10.1016/j.talo.2025.100517

Suvarna P. Dhongade , Surya Teja Malkapuram , Shirish H. Sonawane , Sivakumar Manickam

This study reports the development of high-performance cellulose acetate (CA) membranes embedded with MIL-53(Fe), a metal organic framework (MOF), for efficient dye removal from wastewater. MIL-53(Fe) was synthesized via solvothermal methods and comprehensively characterized using XRD, FTIR, FESEM, TEM, BET, and TGA analyses. XRD and FTIR confirmed the successful formation and integration of MIL-53(Fe) within the CA matrix, while BET analysis revealed a surface area of 34.16 m²/g and pore diameter of 3.42 nm. Electron microscopy demonstrated well-defined polyhedral morphologies and layered crystalline domains, with AFM revealing an increase in surface roughness as MOF loading increased. Composite membranes exhibited enhanced thermal stability and mechanical strength, with tensile strength improving by ∼75 % at 0.75 wt.% MOF due to optimal dispersion; higher loadings led to agglomeration and performance decline. The CA/MIL-0.75 membrane achieved superior water permeability and dye rejection, removing 96 % of Safranin-O and 98 % of Direct Yellow-12. Surface densification, improved porosity, and increased hydrophilicity contributed to enhanced separation performance. Real wastewater treatment trials confirmed effective pollutant removal from textile effluent and greywater. These findings underscore the potential of MIL-53(Fe)-modified CA membranes as robust, thermally stable, and efficient materials for sustainable water purification applications.

本研究报道了一种嵌入金属有机骨架（MOF） MIL-53（Fe）的高性能醋酸纤维素（CA）膜的开发，用于高效去除废水中的染料。采用溶剂热法合成了MIL-53(Fe)，并用XRD、FTIR、FESEM、TEM、BET和TGA对其进行了综合表征。XRD和FTIR证实MIL-53（Fe）在CA基体内成功形成并整合，BET分析显示MIL-53（Fe）的表面积为34.16 m2/g，孔径为3.42 nm。电子显微镜显示了明确的多面体形态和层状晶体域，原子力显微镜显示表面粗糙度随着MOF负载的增加而增加。复合膜表现出增强的热稳定性和机械强度，在0.75 wt.% MOF时，由于最佳分散，拉伸强度提高了约75%；较高的负载导致团聚和性能下降。CA/MIL-0.75膜具有优异的透水性和染料去除率，可去除96%的Safranin-O和98%的Direct Yellow-12。表面致密化、孔隙度改善和亲水性增加有助于提高分离性能。实际废水处理试验证实了对纺织废水和中水的有效去除。这些发现强调了MIL-53（Fe）修饰的CA膜作为耐用、热稳定和高效的材料的潜力，可用于可持续的水净化应用。

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引用次数: 0

Recent advancements in microcystin detection: A comprehensive review of immunosensors and nucleic acid-based biosensors for efficient monitoring in drinking water and human samples 微囊藻毒素检测的最新进展：用于饮用水和人体样本有效监测的免疫传感器和基于核酸的生物传感器的综合综述

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-05-29 DOI: 10.1016/j.talo.2025.100495

Anahas Perianaika Matharasi Antonyraj , Prasannabalaji Nainangu , Ayyakannu Sundaram Ganeshraja , Ajay Guru , Kumaran Subramanian , Manju Dhakad Tanwar , Vairavel Parimelazhagan , A. Santhana Krishna Kumar

Cyanobacteria produce harmful peptides called microcystins (MCs), which are a major concern for the public's health and the water supply. The safety of the water supply depends on the accurate identification of these toxins. A state-of-the-art immunosensor that immobilizes MC variants targeted by monoclonal antibodies onto nanostructured substrates allows for fast and selective detection of MC, as discussed in this review article. Incorporating nanomaterials such as gold nanoparticles and carbon nanotubes, the detector improves the effectiveness of the signal, allowing it to identify as low as 0.05 ppb with a response time of <10 min. With its stable performance over time, this type of sensor is excellent for field deployment and ongoing surveillance. Miniaturizing the device, improving its multifaceted tracking capabilities, and testing it in real-world events are the main goals of future research. The integration of novel technologies and nanomaterials is highlighted in the review as an approach to enhance sensitivity, accessibility, and exchange of data in real-time. Miniaturized portable devices, enhancement of signals, and connection with the Internet of Things are highlighted as significant innovations in environmental monitoring and public health protection. To protect ecosystems and public health, next-generation biosensing technologies could decrease MC contamination in water bodies.

蓝藻产生一种叫做微囊藻毒素（MCs）的有害肽，这是公众健康和供水的一个主要问题。供水的安全取决于这些毒素的准确识别。如本文所述，一种先进的免疫传感器可以将单克隆抗体靶向的MC变体固定在纳米结构的底物上，从而可以快速和选择性地检测MC。结合纳米材料，如金纳米颗粒和碳纳米管，探测器提高了信号的有效性，使其能够识别低至0.05 ppb的响应时间为10分钟。随着时间的推移，其稳定的性能，这种类型的传感器非常适合现场部署和持续监测。未来研究的主要目标是使设备小型化，提高其多方面的跟踪能力，并在现实世界的事件中进行测试。新技术和纳米材料的整合在综述中被强调为一种提高灵敏度、可及性和实时数据交换的方法。小型化便携式设备、增强信号和与物联网的连接被强调为环境监测和公共健康保护的重大创新。为了保护生态系统和公众健康，下一代生物传感技术可以减少水体中的MC污染。

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引用次数: 0

Design and synthesis of 8-nitroquinoline azine D-π-A module chemosensors: Fluorogenic onsite detection of sarin gas mimic- DCP 8-硝基喹啉嗪D-π- a模块化学传感器的设计与合成：沙林气体模拟物- DCP的荧光现场检测

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-06-29 DOI: 10.1016/j.talo.2025.100508

Sentamil Selvi Ramasamy , Kaviyarasu Adhigaman , Vandana Nandakumar , Akilesh Muralidharan , Shankar Ramasamy , Suresh Thangaraj

Organophosphorus (OP) chemical warfare agents are highly toxic and directly interrupts the acetylcholine esterase (AChE) enzyme activity in the central nervous system while exposed to those lethal substances. Detection of OP agents is challenging one and existing analytical methods are also having limitations and tedious operating procedures. In this context, we designed two ICT based 8-nitro substituted quinoline unsymmetrical azine sensors (NQH and NQH-1) for the detection of sarin mimic diethylchlorophosphate (DCP) nerve agent. The developed sensors are shown significant orange region emissive responses to DCP molecule in “TURN ON” manner with reliable red shifts. The limit of detection for the probes NQH and NQH-1 were in micromolar level found to be 1.5 and 3.2 µM respectively. The consequent mechanistic pathway proposed for the sensing systems are PET combined with ICT for NQH, while the NQH-1 system functions through ESIPT coupled with ICT processes. The proposed mechanistic gateway further elucidated through ¹H NMR, ³¹P NMR titrations in addition to the dynamics of DFT. Notably, the proposed D-π-A module chemosensors have good fluorescent module for instant field level recognition of nerve agents (surrogates). The significance of the research found in the sensing platform has been achieved by the fabrication of cotton swab and paper disc kits for the real time analysis.

有机磷（OP）化学战剂具有剧毒，当暴露于这些致命物质时，会直接中断中枢神经系统的乙酰胆碱酯酶（AChE）酶活性。OP试剂的检测是一项具有挑战性的工作，现有的分析方法也存在局限性和繁琐的操作程序。在此背景下，我们设计了两个基于ICT的8-硝基取代喹啉不对称嘧啶传感器（NQH和NQH-1），用于检测沙林模拟二乙基氯磷酸（DCP）神经毒剂。所研制的传感器对DCP分子表现出明显的橙色区发射响应，具有可靠的红移。探针NQH和NQH-1在微摩尔水平上的检出限分别为1.5和3.2µM。由此提出的传感系统的机制途径是PET结合ICT实现NQH，而NQH-1系统通过ESIPT结合ICT过程发挥作用。通过1H NMR， 31P NMR滴定以及DFT动力学进一步阐明了所提出的机制通道。值得注意的是，所提出的D-π-A模块化学传感器具有良好的荧光模块，可用于神经毒剂（替代物）的即时现场水平识别。通过制作用于实时分析的棉签和纸盘套件，实现了在传感平台上发现的研究意义。

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引用次数: 0

High-performance ratiometric optical oxygen sensor fabricated via 3Dprinted silicone for biomedical applications 用于生物医学应用的高性能比例光学氧传感器由3d打印硅胶制成

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-07-05 DOI: 10.1016/j.talo.2025.100513

Rispandi , Cheng-Shane Chu , Abdulfatah Abdu Yusuf , Muhammad Imam Ammarullah

A new design of ratiometric optical oxygen sensors demonstrates considerable potential for applications across medical, environmental, and industrial fields. These sensors are recognized for their high sensitivity, specificity, and resistance to interference from external environmental variables. This research introduces an innovative fabrication method using silicone-based 3D printing technology to develop such sensors. The approach integrates a silicone matrix with oxygen-sensitive dyes, specifically platinum(II) meso‑tetrakis(pentafluorophenyl)porphyrin (PtTFPP) or tris(4,7-diphenyl-1,10-phenanthroline), Ru(dpp)₃²⁺, along with reference dyes such as Rhodamine 110 or 7-amino-4-trifluoromethyl coumarin, which are unaffected by oxygen. These phosphorescent compounds are blended into the silicone medium by manual stirring. Using a 405 nm LED as the excitation source, it was verified that the emission spectra of the sensing and reference dyes do not overlap, allowing reliable oxygen detection via a ratiometric method. The effectiveness of the sensors is evaluated by comparing the intensity ratio I_N₂/I_O₂, where I_N₂ and IO₂ correspond to the luminescent outputs in nitrogen and oxygen atmospheres, respectively. The sensors embedded with PtTFPP and Ru(dpp)₃²⁺ show linear Stern–Volmer behavior, with sensitivity values of about 69 and 12, respectively. Furthermore, the PtTFPP-based sensor exhibits response times of 41 s and 64 s for oxygen increase and decrease transitions, respectively, while the Ru(dpp)₃²⁺-based sensor records 45 and 68 s for the same changes. This ratiometric sensing approach enhances detection sensitivity and response consistency, minimizing variations caused by fluctuations in light source intensity or fiber optics. Combining ratiometric oxygen sensing with 3D-printed silicone structures provides a robust foundation for developing next-generation oxygen sensor technologies with improved capabilities. The silicone matrix's biocompatible nature and the sensor components' high performance are a significant step toward integrating additive manufacturing and optical sensing in developing reliable, miniaturized biomedical sensors for future healthcare technologies.

一种新设计的比率光学氧传感器在医疗、环境和工业领域的应用显示出相当大的潜力。这些传感器因其高灵敏度、特异性和对外部环境变量干扰的抵抗力而得到认可。本研究介绍了一种利用硅基3D打印技术开发此类传感器的创新制造方法。该方法将硅基与氧敏感染料集成在一起，特别是铂（II）中四（五氟苯基）卟啉（PtTFPP）或三（4,7-二苯基-1,10-菲罗啉），Ru（dpp）₃2⁺以及参考染料，如罗丹明110或7-氨基-4-三氟甲基香豆素，这些染料不受氧影响。这些磷光化合物通过人工搅拌混合到硅树脂介质中。利用405 nm LED作为激发源，验证了感测染料和参比染料的发射光谱不重叠，从而通过比例法实现了可靠的氧检测。通过比较强度比IN₂/IO₂来评估传感器的有效性，其中IN₂和IO₂分别对应于氮和氧气氛下的发光输出。嵌入PtTFPP和Ru（dpp）₃2⁺的传感器表现出线性的Stern-Volmer行为，灵敏度分别约为69和12。此外，基于pttfpp的传感器对氧的增加和减少变化的响应时间分别为41 s和64 s，而基于Ru（dpp）₃2⁺的传感器对相同变化的响应时间分别为45 s和68 s。这种比例传感方法提高了检测灵敏度和响应一致性，最大限度地减少了由光源强度或光纤波动引起的变化。将比例氧传感与3d打印有机硅结构相结合，为开发具有改进功能的下一代氧传感器技术提供了坚实的基础。有机硅基质的生物相容性和传感器组件的高性能是集成增材制造和光学传感的重要一步，为未来的医疗保健技术开发可靠的小型化生物医学传感器。

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引用次数: 0

Nanogram-level green spectrofluorimetric method for simultaneous quantification of tadalafil and silodosin in pharmaceuticals, environmental matrices and content uniformity testing 纳克级绿色荧光光谱法同时定量药品、环境基质中他达拉非和西洛多辛含量均匀性试验

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-08-27 DOI: 10.1016/j.talo.2025.100534

Aya Saad Radwan , Mohamed Hefnawy , Talha Bin Emran , Mohamed M. Salim

A novel, eco-friendly spectrofluorimetric method has been developed and validated for the first time for simultaneous determination of tadalafil (TDF) and silodosin (SLD) in pharmaceutical dosage forms and environmental water samples without the need for prior separation. This innovative approach exploits the emission fluorescence of both drugs, employing excitation at 270 nm and emission detection at 328 nm for TDF and 454 nm for SLD. The method demonstrated excellent linearity across the concentration ranges of 50.0–1200.0 ng mL^-1 for TDF and 1.0–300.0 ng mL^-1 for SLD (r > 0.9999), with remarkable limits of detection of 10.94 ng mL^-1 and 0.28 ng mL^-1, respectively. The high sensitivity and precision (RSD < 0.70 %), with recovery rates ranging from 99.50 % to 101.00 % in pharmaceuticals and 97.31 % to 102.83 % in environmental water emphasize the method’s robustness and practical applicability for therapeutic drug monitoring and environmental surveillance. Application to content uniformity testing of tablet formulations, in accordance with British Pharmacopoeia guidelines, further demonstrates the method’s versatility. Greenness assessments using the AGREE and complex moGAPI metrics, alongside operational efficiency evaluation through the Click Analytical Chemistry Index (CACI), confirm the method’s sustainability and minimal environmental impact. Fully compliant with ICH Q2(R1) guidelines, this validated protocol offers a pioneering, rapid, and sustainable alternative for routine quality control and environmental monitoring.

本文首次建立了一种新型、环保的荧光光谱法，可同时测定药物剂型和环境水样中的他达拉非（TDF）和西洛多辛（SLD），而无需事先分离。这种创新的方法利用了两种药物的发射荧光，采用270 nm的激发和328 nm的发射检测，TDF和454 nm的SLD。该方法在TDF浓度范围为50.0 ~ 1200.0 ng mL-1和SLD浓度范围为1.0 ~ 300.0 ng mL-1 （r > 0.9999）具有良好的线性关系，检测限分别为10.94 ng mL-1和0.28 ng mL-1。该方法具有较高的灵敏度和精密度（RSD < 0.70%），在药品和环境水中的回收率分别为99.50% ~ 101.00%和97.31% ~ 102.83%，具有较好的鲁棒性和实用性。应用于片剂配方的含量均匀性测试，根据英国药典指南，进一步证明了该方法的通用性。使用AGREE和复杂的moGAPI指标进行的绿色评估，以及通过点击分析化学指数（CACI）进行的操作效率评估，证实了该方法的可持续性和最小的环境影响。该方案完全符合ICH Q2（R1）指南，为常规质量控制和环境监测提供了一种开创性、快速和可持续的替代方案。

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引用次数: 0

Plasmonic fingerprinting: next-generation SERS architectures for sensitive heavy metal quantification 等离子体指纹识别：用于敏感重金属量化的下一代SERS架构

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 Epub Date: 2025-09-09 DOI: 10.1016/j.talo.2025.100554

Muhammad Sarkawi , Rupam Sharma , Geetha Venkatesan , Tripti Ahuja , Soumyanti Panda , Jai Prakash , Hideki Kuramitz , K.S. Shalini devi

The global heavy metal crisis demands sensing technologies that transcend traditional analytical limitations, positioning Surface-Enhanced Raman Scattering (SERS) as the transformative solution for real-world environmental and health monitoring challenges. This comprehensive review illuminates the sophisticated engineering principles underlying modern SERS architectures, where nanoscale precision meets molecular recognition to achieve detection limits that challenge the boundaries of single-molecule spectroscopy. Additionally, a systematic analysis of various SERS sensing strategies, including label-free approaches utilizing direct metal-nanoparticle interactions, molecular probe-based systems employing Raman-active chelating agents, and advanced functionalized nanoparticle platforms incorporating DNA aptamers, peptides, and polymeric recognition elements are discussed. Representative case studies include the successful detection of mercury in contaminated fish samples at sub-regulatory levels, lead quantification in urban drinking water systems, and arsenic monitoring in rice cultivation areas, showcasing the versatility and reliability of SERS-based approaches across diverse analytical challenges. The review concludes with a progressive perspective on the merging of SERS technology with microfluidics, Internet of Things architectures, and distributed sensing networks that will replace the current approach of testing samples periodically with continuous monitoring of heavy metals at specific locations.

全球重金属危机需要超越传统分析限制的传感技术，将表面增强拉曼散射（SERS）定位为现实世界环境和健康监测挑战的变革性解决方案。这篇全面的综述阐明了现代SERS架构背后的复杂工程原理，其中纳米级精度满足分子识别，以实现挑战单分子光谱边界的检测极限。此外，本文还讨论了各种SERS传感策略的系统分析，包括利用直接金属-纳米颗粒相互作用的无标记方法，利用拉曼活性螯合剂的基于分子探针的系统，以及结合DNA适体、肽和聚合物识别元件的高级功能化纳米颗粒平台。代表性案例研究包括成功检测亚监管水平受污染鱼类样本中的汞，城市饮用水系统中的铅量化以及水稻种植区域的砷监测，展示了基于sers的方法在各种分析挑战中的多功能性和可靠性。该综述总结了SERS技术与微流体、物联网架构和分布式传感网络相结合的进步观点，这些技术将取代目前定期检测样品并在特定位置连续监测重金属的方法。

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