Talanta Open最新文献

{"title":"Nanogram-level green spectrofluorimetric method for simultaneous quantification of tadalafil and silodosin in pharmaceuticals, environmental matrices and content uniformity testing","authors":"Aya Saad Radwan ,&nbsp;Mohamed Hefnawy ,&nbsp;Talha Bin Emran ,&nbsp;Mohamed M. Salim","doi":"10.1016/j.talo.2025.100534","DOIUrl":"10.1016/j.talo.2025.100534","url":null,"abstract":"<div><div>A novel, eco-friendly spectrofluorimetric method has been developed and validated for the first time for simultaneous determination of tadalafil (TDF) and silodosin (SLD) in pharmaceutical dosage forms and environmental water samples without the need for prior separation. This innovative approach exploits the emission fluorescence of both drugs, employing excitation at 270 nm and emission detection at 328 nm for TDF and 454 nm for SLD. The method demonstrated excellent linearity across the concentration ranges of 50.0–1200.0 ng mL^-1 for TDF and 1.0–300.0 ng mL^-1 for SLD (r &gt; 0.9999), with remarkable limits of detection of 10.94 ng mL^-1 and 0.28 ng mL^-1, respectively. The high sensitivity and precision (RSD &lt; 0.70 %), with recovery rates ranging from 99.50 % to 101.00 % in pharmaceuticals and 97.31 % to 102.83 % in environmental water emphasize the method’s robustness and practical applicability for therapeutic drug monitoring and environmental surveillance. Application to content uniformity testing of tablet formulations, in accordance with British Pharmacopoeia guidelines, further demonstrates the method’s versatility. Greenness assessments using the AGREE and complex moGAPI metrics, alongside operational efficiency evaluation through the Click Analytical Chemistry Index (CACI), confirm the method’s sustainability and minimal environmental impact. Fully compliant with ICH Q2(R1) guidelines, this validated protocol offers a pioneering, rapid, and sustainable alternative for routine quality control and environmental monitoring.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100534"},"PeriodicalIF":3.7,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Material matters: evaluating polymer 96-well plates to reduce DAMGO loss in LC-MS/MS-based in vitro μ-opioid structure affinity assay","authors":"Gaia Alluisetti ,&nbsp;Emma Van Impe ,&nbsp;Wolfgang Weinmann ,&nbsp;Katharina Elisabeth Grafinger","doi":"10.1016/j.talo.2025.100543","DOIUrl":"10.1016/j.talo.2025.100543","url":null,"abstract":"<div><div>The peptide DAMGO has been used for decades to determine binding affinity at the μ-opioid receptor in numerous types of receptor binding assays utilizing radiolabeled DAMGO, and more recently, liquid chromatography tandem mass spectrometry (LC-MS/MS)-based procedures with unlabeled DAMGO. While previously there have been numerous reports of non-specific adsorption (NSA) at plastic surfaces of various peptides, this study reports for the first time the NSA of DAMGO at polymer 96-well plates used in receptor binding affinity assays. In the present study, seven different types of polypropylene and one modified polystyrene 96-well plates were assessed for DAMGO NSA. The number of solution transfers, two different incubation temperatures and times were evaluated. NSA and DAMGO signal loss were observed for six out of eight tested plates. Best results were obtained with Thermo Scientific (Cat. No 60180-P133) 96-well deep-well plates, for which no significant signal deviation could be observed compared to the blanks even after six sequential transfers. Coated microtiter plates from Greiner Bio-One (Cat. No 655901) showed significant signal enhancement compared to the blanks, which may also cause issues when performing quantitative analysis of results. This study highlights the necessity of testing laboratory equipment for NSA during assay development, since peptide or analyte adsorption may not be immediately apparent, leading to the issue going unnoticed.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100543"},"PeriodicalIF":3.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in photoelectrochemical sensing of tetracyclines","authors":"Yali Cui ,&nbsp;Yanyuan Zhang ,&nbsp;Lin Wang ,&nbsp;Yuanqiang Hao","doi":"10.1016/j.talo.2025.100542","DOIUrl":"10.1016/j.talo.2025.100542","url":null,"abstract":"<div><div>Tetracyclines (TCs) are widely used antibiotics whose residues pose environmental and health risks, necessitating sensitive and selective detection. Photoelectrochemical (PEC) sensors have emerged as promising analytical tools due to their high sensitivity, low background noise, and cost-effective instrumentation. This review systematically summarizes recent advances in PEC sensing of TCs. We begin by categorizing the photoactive materials employed in PEC sensing and examining their structural design strategies, heterojunction engineering, and photoelectric properties. Subsequently, we elaborate on the fundamental signal transduction mechanisms, including the intrinsic redox activity of TCs, steric hindrance-induced current suppression, and coordination-mediated modulation of interfacial charge transfer. Furthermore, we highlight representative PEC sensing systems reported for TCs detection, classifying them based on recognition strategies such as aptamer binding, molecular imprinting, electrostatic adsorption, and site-specific coordination—each linked to distinct signal generation modes. Finally, current limitations and promising strategies for further performance enhancement of PEC sensors are discussed. This review provides a comprehensive perspective on PEC strategies for TCs detection and offers guidance for future innovations in environmental and food safety monitoring.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100542"},"PeriodicalIF":3.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144911961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality-by-design optimized HPLC approach for the therapeutic drug monitoring of isosorbide dinitrate and sildenafil in human plasma samples","authors":"Mohamed A. Momtaz ,&nbsp;Hanaa S. El-Desoky ,&nbsp;Ahmed Rehab ,&nbsp;Fathalla Belal","doi":"10.1016/j.talo.2025.100540","DOIUrl":"10.1016/j.talo.2025.100540","url":null,"abstract":"<div><div>Isosorbide dinitrate (ISDN) and sildenafil citrate (SIL) are reported to have a potential drug-drug interaction when co-administered clinically. <strong>ISDN</strong> is indicated as first-line therapy for the long-term management of chronic stable angina. <strong>Sildenafil</strong> is the first oral treatment for erectile dysfunction. It is contra-indicated in patients who may require organic nitrates, such as ISDN, because this combination may cause a sudden drop in blood pressure by inhibiting the phosphodiesterase type 5 enzyme. Additionally, sildenafil is effective in treating cardiovascular disorders secondary to endothelial dysfunctions. Therefore, a sensitive, simple, and selective HPLC method is urgently needed for their simultaneous determination in plasma samples for monitoring their therapeutic blood concentration levels in emergency cases. The current study introduces the first HPLC approach for their simultaneous estimation in less than 10 minutes. The optimal separation was achieved using Nova-Pack® C18, 4 µm column at room temperature, with a mobile phase consisting of acetonitrile and acetate buffer (5 mM; pH 5, 39:61 % v/v), delivered at a flow rate of 1.1 mL/min. The injection volume was set at 50 µL, and detection was accomplished at 214 nm. The method demonstrated excellent linearity for ISDN(0.01–10.0µg/mL) and SIL (0.025–10.0µg/mL), with LOQ of 0.01 µg/mL and 0.020 µg/mL, respectively. The proposed method was successfully applied to analyze spiked human samples with recovery rates (104.9 and 105.55%) for ISDN and SIL respectively, confirming its suitability for bio-analytical use. Utilizing the quality-by- design (QbD) approach adopting the two-level full factorial design allowed the optimization of the experimental conditions of the proposed method. The method’s greenness was evaluated using Analytical Eco-Scale, Green Analytical Procedure Index (GAPI), and Analytical Greenness (AGREE). Validation of the proposed method was performed in accordance with ICH and US FDA guidelines.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100540"},"PeriodicalIF":3.7,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on biosensor approaches for the detection of hazardous elements in water","authors":"Nidhi Chauhan ,&nbsp;Nishtha Sah ,&nbsp;Kirti Saxena ,&nbsp;Utkarsh Jain","doi":"10.1016/j.talo.2025.100536","DOIUrl":"10.1016/j.talo.2025.100536","url":null,"abstract":"<div><div>Water is the basis of life on Earth, but due to increased pollution and habitat degradation, water scarcity is increasing day by day. Therefore, it is necessary to detect water pollutants to save water and aquatic diversity. Monitoring of toxic elements present in wastewater effluents is an important factor for further treatment to make it pollutant-free. While various methods have been developed for monitoring these toxic elements, biosensors can overcome the limitations of traditional methods. Biosensors are highly sensitive, selective, low in cost, and simple to use. In this review, we have provided an overview of reported biosensors for the detection of various types of toxic elements that cause water toxicity. A summary of these toxic elements has also been discussed herein. This review aims to provide an overview of advances in biosensing technology used for water monitoring.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100536"},"PeriodicalIF":3.7,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An analytical method to quantify tetrabromobisphenol A (TBBPA) and its conjugated metabolites in rat serum or other biological samples","authors":"Mette Stub,&nbsp;Mikael Pedersen,&nbsp;Louise Ramhøj,&nbsp;Kit Granby,&nbsp;Terje Svingen,&nbsp;Agnieszka Anna Niklas","doi":"10.1016/j.talo.2025.100537","DOIUrl":"10.1016/j.talo.2025.100537","url":null,"abstract":"<div><div>Brominated flame retardants (BFRs) are widely used to enhance fire resistance in consumer and commercial products, but often leach into the environment due to their surface application. Tetrabromobisphenol A (TBBPA), the most applied BFR, has been detected in human serum, urine, and breast milk. TBBPA is readily absorbed from the gastrointestinal tract, undergoing first-pass hepatic metabolism to form the two major metabolites TBBPA-sulfate and TBBPA-glucuronide. Despite evidence linking TBBPA exposure to developmental toxicity, neurobehavioral effects, uterine cancers, and endocrine disrupting potential via thyroid hormone interference, exposure assessments remain challenging. Current methods rely heavily on direct quantification of TBBPA and its metabolites using liquid chromatography-mass spectrometry (LC-MS), but a lack of commercially available TBBPA metabolites at analytical standards is a limiting factor. This study introduces a method to rapidly quantify TBBPA, and after enzymatic deconjugation, its metabolites using LC-Orbitrap. Using this method, TBBPA-glucuronide was the only metabolite detected in rat serum following oral exposure. Native TBBPA was obtained using enzymatic deconjugation of serum. The protocol simplifies exposure evaluation by offering a practical, and rapid, approach for quantifying TBBPA in biological samples.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100537"},"PeriodicalIF":3.7,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144911960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-invasive electrochemical sensing of toluidine blue in human saliva using silver conductive ink stabilized on paper substrate: A new platform in portable sensor technology","authors":"Rokhsareh Ebrahimi ,&nbsp;Mohammad Hasanzadeh ,&nbsp;Nasrin Shadjou","doi":"10.1016/j.talo.2025.100539","DOIUrl":"10.1016/j.talo.2025.100539","url":null,"abstract":"<div><div>In this study, we developed a novel paper-based electrochemical microsensor for label-free TB detection using conducting silver nano-inks stabilized on the on the surface of photography paper. The silver ink stabilized on the surface of paper which maintained suitable conductivity and structural integrity after repeated bending, indicating excellent flexibility. In addition, the electrodes maintained stable conductivity (resistance of 5.2 Ω) and LED performance at 100 °C, indicating the thermal flexibility of the cellulose modified by ink due to the strong interaction of AgNPs with cellulose substrate. Also, exposure to moisture had minimal effect on the electrical properties, such that the brightness and LED resistance (1.7 Ω, ΔR = 2.7 Ω) remained stable, confirming the moisture resistance of the ink. The electrodes also dried immediately after drawing without requiring more time or temperatures, which was one of the advantages of our synthetic ink. The developed microscale sensor can accurately detect the concentration of salivary TB in the linear range of 10–1000 nM, with a lower limit of quantification of 10 nM, which is considered as suitable range for clinical and diagnostic applications.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100539"},"PeriodicalIF":3.7,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144904571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Portable electrochemiluminescent detection system with 3D printed materials and mobile phone detection","authors":"Patrick J. Herchenbach ,&nbsp;Ethan Gomez ,&nbsp;Dale Mee ,&nbsp;Charles S. Henry ,&nbsp;Erin M. Gross","doi":"10.1016/j.talo.2025.100535","DOIUrl":"10.1016/j.talo.2025.100535","url":null,"abstract":"<div><div>3D printing offers advantages and novel opportunities for the analytical chemistry laboratory. The method presented here used 3D printed materials as the substrates for electrochemiluminescent sensors and used 3D printing to construct a portable housing for the sensors that not only aligns the sensor to the detector but provides a light-tight environment for the luminescence measurement. This work evaluated four different 3D-printed plastics as substrate materials for sensors fabricated from stencil-printed carbon-ink electrodes (SPCE’s). SPCE chips were fabricated that incorporated an electrochemical cell onto three different plastics printed via fused deposition modeling and a plastic printed using stereolithography printing. The chips were used to develop an ECL detection method with the luminophore tris(2,2′-bipyridyl)ruthenium(II) [Ru(bpy)₃²⁺] and 2-(dibutylamino)ethanol (DBAE). The ECL reaction between Ru(bpy)₃²⁺ and DBAE was used to optimize a chip-based ECL detection method for amine-containing species. The limits of detection (S/<em>N</em> = 3) for DBAE on the four different substrates were similar, ranging from 3 – 4 μM. The method was applied to the detection of the biogenic amine spermidine. The method had a detection limit of ∼130 μM for spermidine. With goals of accessibility and portability, the method also utilized a mobile phone detector, and portability was demonstrated with the use of a USB power supply to generate the voltage for the ECL reaction.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100535"},"PeriodicalIF":3.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144911959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed carbon black-based electrode for simultaneous determination of ascorbic acid and kojic acid in dermo-cosmetic products","authors":"Larissa Gil Lucas ,&nbsp;Eduardo Constante Martins ,&nbsp;Pãmyla Layene dos Santos ,&nbsp;João Paulo Winiarski ,&nbsp;Edson Roberto Santana ,&nbsp;Iolanda Cruz Vieira ,&nbsp;Almir Spinelli","doi":"10.1016/j.talo.2025.100532","DOIUrl":"10.1016/j.talo.2025.100532","url":null,"abstract":"<div><div>A miniaturized and disposable poly(lactic acid)-carbon black-based working electrode (3D CB-PLA) was developed using 3D printing technology through fused filament fabrication to be applied to the simultaneous analysis of the antioxidant compounds ascorbic acid (vitamin C) and kojic acid. A previous chemical/electrochemical activation of the 3D CB-PLA electrode was carried out in 1.0 mol L⁻¹ NaOH, applying a fixed potential of +1.0 V <em>vs</em>. Ag/AgCl, KCl_(sat) for 1800 s. This procedure was necessary to remove a smooth layer of PLA polymer on the electrode surface and to allow the carbon black conductive sites to be exposed. Morphological and spectroscopic characterizations performed before and after activation revealed a rougher surface with an increased presence of oxygenated functional groups, attributed to both the activation process and the enhanced exposure of carbon black. Using cyclic voltammetry in 0.1 mol L⁻¹ Britton-Robinson buffer (pH 7.0), the irreversible oxidation reactions of ascorbic and kojic acids were observed at +0.30 and +0.87 V <em>vs</em>. Ag/AgCl, KCl_(sat), respectively. With the experimental conditions optimized, calibration curves were constructed for both analytes by square wave voltammetry, with detection limits of 4.80 and 5.40 µmol L⁻¹ for ascorbic acid and kojic acid, respectively. The activated 3D CB-PLA electrode enabled accurate and precise simultaneous quantification of both antioxidant compounds in facial whitening creams, demonstrating its effectiveness and analytical potential.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100532"},"PeriodicalIF":3.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable upcycling of watermelon peels into a nanosensor for the selective detection of mefenamic acid, a nonsteroidal anti-inflammatory drug with antioxidant properties, in biofluids and pharmaceutical formulations","authors":"Ahmed A. Shokeer,&nbsp;Nora A. Abdallah,&nbsp;Fawzia A. Ibrahim","doi":"10.1016/j.talo.2025.100533","DOIUrl":"10.1016/j.talo.2025.100533","url":null,"abstract":"<div><div>Mefenamic acid has recently been identified to possess antioxidant properties, expanding its potential applications beyond traditional pain relief. Emerging studies suggest its possible role in the prevention or management of Alzheimer’s disease through its anti-inflammatory and oxidative stress-reducing effects. This study represents an innovative, highly green eco-friendly method for determination of mefenamic acid in plasma, urine and in its pharmaceutical forms. It involves the transformation of waste watermelon peels into a highly fluorescent switch off nanosensor. For the first time, carbon quantum dots were synthesized from watermelon peel in one step via in situ microwave-assisted carbonization process, completed within 17.0 min without the addition of any external chemicals. The synthesized carbon quantum dots were found to have good fluorescence (λex/em 330.0/420.0 nm), high water solubility and good stability. The synthesized carbon dots were characterized using fluorescence spectroscopy, UV–Visible Spectroscopy, X-ray photoelectron spectroscopy, Zeta potential analysis; Transmission electron microscopy and Fourier transform infrared. The formed Carbon dots appeared to have average particle size of 3.69 - 6.19 nm and their shape was spherical. Mefenamic acid, a nonsteroidal anti-inflammatory anthranilic acid derivative with antioxidant properties, was determined using the synthesized carbon quantum dots by inner filter effect (IFE) and static fluorescence quenching mechanism. The quenching effect on the produced carbon quantum dots was utilized for determination of mefenamic acid over the range of (0.50 – 20.0 µg/mL). The % recovery of the developed method was found to be 100.03 ± 0.776 in pure form, 99.973 ± 0.713 in capsule, 99.70 ± 3.740 in plasma and 99.968 ± 4.005 in urine. Determination of mefenamic acid in the presence of different species indicated high selectivity of the suggested method. The validity of the proposed method was assessed according to the ICH recommendations. Different greenness assessment tools were used for evaluation of the proposed method sustainability. By using watermelon peel, typically regarded as waste, this method offers a green and sustainable route for synthesizing carbon quantum dots from natural sources. Also, using water as solvent without using any other organic solvents; it gives a great advantage to our work as it does not produce any chemical or harmful waste, which makes it safe for the analyst and earth.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100533"},"PeriodicalIF":3.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}