Journal of Oral Biosciences最新文献_第8页

Synergistic effect of Toll-like receptor 2 ligands and alendronate on proinflammatory cytokine production in mouse macrophage-like RAW-ASC cells is accompanied by upregulation of MyD88 expression Toll样受体2配体和阿仑膦酸钠对小鼠巨噬细胞样RAW-ASC细胞促炎细胞因子产生的协同作用伴随着MyD88表达的上调。

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-06-01 DOI: 10.1016/j.job.2024.04.003

Reiko Akaho , Yusuke Kiyoura , Riyoko Tamai

Objectives

Toll-like receptors (TLRs) recognize whole cells or components of microorganisms. Alendronate (ALN) is an anti-bone-resorptive drug that has inflammatory side effects. The aim in this study was to examine whether ALN augments TLR2 ligand-induced proinflammatory cytokine production using mouse macrophage-like RAW264.7 cells transfected with murine apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) gene (hereafter, referred to as “RAW-ASC cells”).

Methods

RAW-ASC cells were pretreated with or without ALN and then incubated with or without TLR2 ligands. The levels of secreted mouse IL-1α, IL-1β, IL-6, and tumor necrosis factor-α (TNF-α) in culture supernatants and the activation of activator protein-1 (AP-1) or nuclear factor-κB (NF-κB) were measured using enzyme-linked immunosorbent assay (ELISA). The expressions of myeloid differentiation factor 88 (MyD88), caspase-11, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3), ASC, NF-κB p65, and actin were analyzed via Western blotting. TLR2 expression was analyzed using flow cytometry.

Results

ALN substantially upregulated the Pam₃CSK₄-induced release of IL-1α, IL-1β, IL-6, and TNF-α and MyD88 expression in RAW-ASC cells. ST-2825, a MyD88 inhibitor, inhibited the ALN-augmented release of these cytokines. Pretreatment with ALN augmented Pam₃CSK₄-induced NF-κB activation in RAW-ASC cells and upregulated AP-1 activation. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) S protein and ALN synergically upregulated the release of IL-1α, IL-1β, IL-6 and TNF-α in RAW-ASC cells.

Conclusions

Our findings suggest that ALN augments TLR2 ligand-induced proinflammatory cytokine production via the upregulation of MyD88 expression, and this augmentation is accompanied by the activation of NF-κB and AP-1 in RAW-ASC cells.

目的类oll受体（TLRs）可识别整个细胞或微生物的成分。阿仑膦酸钠（ALN）是一种抗骨吸收药物，具有炎症副作用。本研究的目的是利用转染了含有卡巴酶募集结构域（ASC）基因的小鼠凋亡相关斑点样蛋白的小鼠巨噬细胞样 RAW264.7 细胞（以下简称 "RAW-ASC 细胞"），研究 ALN 是否会增加 TLR2 配体诱导的促炎细胞因子的产生。用酶联免疫吸附试验（ELISA）测定培养上清液中小鼠分泌的 IL-1α、IL-1β、IL-6 和肿瘤坏死因子-α（TNF-α）的水平，以及活化蛋白-1（AP-1）或核因子-κB（NF-κB）的活化情况。通过 Western 印迹分析了髓样体分化因子 88（MyD88）、caspase-11、核苷酸结合寡聚域样受体家族含 pyrin 域 3（NLRP3）、ASC、NF-κB p65 和肌动蛋白的表达。结果ALN大大提高了Pam3CSK4诱导的IL-1α、IL-1β、IL-6和TNF-α的释放以及RAW-ASC细胞中MyD88的表达。MyD88抑制剂ST-2825抑制了ALN促进的这些细胞因子的释放。ALN预处理增强了Pam3CSK4诱导的NF-κB在RAW-ASC细胞中的活化，并上调了AP-1的活化。我们的研究结果表明，ALN 通过上调 MyD88 的表达增强了 TLR2 配体诱导的促炎细胞因子的产生，这种增强伴随着 RAW-ASC 细胞中 NF-κB 和 AP-1 的活化。

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引用次数: 0

Title Page (with cities) 扉页（含城市）

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-06-01 DOI: 10.1016/S1349-0079(24)00098-7

引用次数: 0

Influence of feeding a soft diet on proteoglycan expression in rat temporomandibular joint discs 喂食软质食物对大鼠颞下颌关节椎间盘蛋白多糖表达的影响

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-06-01 DOI: 10.1016/j.job.2024.05.009

Objectives

Extracellular matrix components play a significant role in maintaining tissue integrity and pathological processes of the temporomandibular joint (TMJ). This study aimed to evaluate the influence of a soft diet on the mRNA expression of proteoglycans and glycosaminoglycans (GAGs) linked to proteoglycan core proteins in rat TMJ discs.

Methods

Thirty 4-week-old male Wistar rats were assigned to one of two groups: a control group fed a regular pellet diet and a soft diet group fed a powdered diet for 4 weeks. The mRNA expression levels of 12 proteoglycans in TMJ discs were evaluated using real-time polymerase chain reaction (PCR). In addition, histomorphometric and biochemical analyses were performed to evaluate the thickness and deoxyribonucleic acid (DNA), GAG, and water content of the TMJ discs.

Results

The TMJ disc thickness in the anterior, intermediate, and posterior bands decreased significantly in the soft diet group. The GAG content decreased significantly in the soft-diet group, whereas no significant differences in DNA content or water content ratio were observed between the groups. Real-time PCR indicated that the expression levels of aggrecan, versican, biglycan, decorin, fibromodulin, lumican, and chondroadherin decreased in the soft diet group. The expression levels of all versican isoforms decreased in the soft diet group.

Conclusions

These results indicate that the biomechanical environment of the TMJ caused by a soft diet is closely related to the expression of proteoglycans in TMJ discs, which may eventually increase the fragility of the TMJ discs.

目的：细胞外基质成分在维持颞下颌关节（TMJ）组织完整性和病理过程中发挥着重要作用。本研究旨在评估软质饮食对大鼠颞下颌关节盘中蛋白聚糖和与蛋白聚糖核心蛋白相连的糖胺聚糖（GAGs）mRNA 表达的影响：将 30 只 4 周大的雄性 Wistar 大鼠分为两组：对照组和软食组，对照组喂食普通颗粒食物，软食组喂食粉状食物 4 周。使用实时聚合酶链反应（PCR）评估了颞下颌关节盘中 12 种蛋白多糖的 mRNA 表达水平。此外，还进行了组织形态计量学和生化分析，以评估颞下颌关节盘的厚度、脱氧核糖核酸（DNA）、凝胶体（GAG）和水分含量：结果：软质饮食组颞下颌关节盘前、中、后带的厚度明显减少。软质饮食组的 GAG 含量明显下降，而 DNA 含量和含水量比在组间无明显差异。实时 PCR 显示，软饮食组中 aggrecan、versican、biglycan、decolin、fibromodulin、lumican 和 chondroadherin 的表达水平下降。软质饮食组中所有 versican 同工酶的表达水平均有所下降：这些结果表明，软质饮食导致的颞下颌关节生物力学环境与颞下颌关节盘中蛋白多糖的表达密切相关，最终可能会增加颞下颌关节盘的脆性。

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引用次数: 0

Spontaneous regeneration after resection of various lengths of hypoglossal nerve in rats 大鼠不同长度舌下神经切除后的自发性再生。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-05-29 DOI: 10.1016/j.job.2024.05.010

Objectives

The objective of this study was to investigate spontaneous neural regeneration and functional recovery after resection of various lengths of the hypoglossal (XII) nerve in adult rats.

Methods

Twelve weeks after XII nerve resection at lengths ranging from 0.0 to 15.8 mm, the tongue deviation angle of rats was measured to evaluate the severity of paralysis; thereafter, the XII neurons in the XII nucleus were labeled with Fluoro-Gold (FG), which was injected into the tongue to visualize regenerated XII neurons re-innervating the tongue muscles.

Results

In the XII nerve-resected rats, the regenerative rates, that is, the percentage of the total number of FG-positive neurons on the injured side relative to that on the uninjured side, were divided into two groups; the regenerative rates were more than 77% and less than 6%, respectively. Upon comparing the two groups, the boundary resection length was approximately 10.0 mm. Moreover, the former and latter groups demonstrated tongue deviation angles less than or greater than 15°, respectively.

Conclusions

The critical nerve gap length for spontaneous neural regeneration was approximately 10.0 mm in XII nerve-resected adult rats, and nerve regeneration occurred in both morphological and functional aspects after resection at less than the critical length.

研究目的本研究旨在调查成年大鼠舌下神经（XII）不同长度切除后的自发性神经再生和功能恢复情况：方法：在大鼠舌下神经（XII）切除0.0-15.8毫米后12周，测量大鼠的舌偏角以评估麻痹的严重程度；然后用氟金（FG）标记XII核中的XII神经元，并将其注入舌部以观察再生的XII神经元重新支配舌肌的情况：结果：切除 XII 神经的大鼠的再生率（即损伤侧 FG 阳性神经元总数占未损伤侧神经元总数的百分比）分为两组，再生率分别为 77% 以上和 6% 以下。两组比较，边界切除长度约为 10.0 毫米。此外，前一组和后一组的舌偏角分别小于或大于 15°：结论：切除 XII 神经的成年大鼠自发性神经再生的临界神经间隙长度约为 10.0 毫米，在小于临界长度时，切除后的神经在形态和功能方面都会发生再生。

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引用次数: 0

Unraveling the cognitive implications among individuals with co-occurring chronic periodontitis and type 2 diabetes mellitus: A cross-sectional study 揭示同时患有慢性牙周炎和 2 型糖尿病患者的认知影响：一项横断面研究。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-05-28 DOI: 10.1016/j.job.2024.05.008

Objectives

Chronic periodontitis and type 2 diabetes mellitus (T2DM) are associated with cognitive decline when examined individually. To gain deeper insight into the combined effects of these conditions on cognitive decline, the present study aimed to examine the cognitive status of individuals with co-occurring T2DM and chronic periodontitis.

Methods

We recruited 220 participants categorized into four groups: Group I, healthy subjects; Group II, individuals with chronic periodontitis; Group III, individuals with T2DM; and Group IV, individuals with both T2DM and chronic periodontitis. Medical histories were recorded for all participants, followed by periodontal examination and evaluation of cognitive status using the Montreal Cognitive Assessment (MoCA) scale. Finger dexterity was assessed using the nine-hole peg test.

Results

A statistically significant increase in the proportion of mild cognitive impairment (MCI) was observed between groups I and IV (p < 0.001). Logistic regression analysis revealed that, among the parameters assessed in this study, the adjusted odds ratio (OR) was significant for age, finger dexterity scores, and co-occurrence of T2DM and periodontitis.

Conclusions

The findings of this study suggest that the co-occurrence of chronic periodontitis and T2DM can have a detrimental effect on the cognitive abilities of an individual. Subsequent research should include longitudinal monitoring of the cognitive status in patients with concurrent conditions during treatment to gain deeper prognostic insights into the relationship between these co-occurring conditions and cognitive decline.

目的：单独研究慢性牙周炎和 2 型糖尿病（T2DM）会导致认知能力下降。为了深入了解这些疾病对认知能力下降的综合影响，本研究旨在调查同时患有 T2DM 和慢性牙周炎的个体的认知状况：我们招募了 220 名参与者，分为四组：方法：我们招募了 220 名参与者，分为四组：第一组，健康受试者；第二组，慢性牙周炎患者；第三组，T2DM 患者；第四组，T2DM 和慢性牙周炎患者。对所有参与者记录病史，然后进行牙周检查，并使用蒙特利尔认知评估（MoCA）量表评估认知状况。使用九孔钉测试评估手指灵活性：结果：在第一组和第四组之间观察到轻度认知障碍（MCI）的比例有明显增加（p）：本研究结果表明，慢性牙周炎和 T2DM 同时存在会对人的认知能力产生不利影响。后续研究应包括在治疗期间对并发症患者的认知状况进行纵向监测，以便更深入地了解这些并发症与认知能力下降之间的预后关系。

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引用次数: 0

Epithelial-mesenchymal transition in oral cancer cells induced by prolonged and persistent Fusobacterium nucleatum stimulation 核酸分枝杆菌长期持续刺激诱导口腔癌细胞发生上皮-间质转化。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-05-21 DOI: 10.1016/j.job.2024.05.006

Objectives

Several studies have reported the effects of Fusobacterium nucleatum stimulation on oral cancer cells. However, given that these studies typically span a stimulation period of three days to eight days, the in vitro studies conducted to date may not fully mimic the oral cancer environment, which involves constant exposure to oral commensal bacteria. This study aimed to elucidate the effects of prolonged and persistent Fusobacterium nucleatum infection on oral cancer cells.

Methods

Human tongue squamous cell carcinoma (SCC) cells were continuously stimulated with Fusobacterium nucleatum for two or four weeks, then experimentally evaluated.

Results

Prolonged, persistent Fusobacterium nucleatum stimulation increased the cells’ proliferative, invasive, and migratory capacities, decreased their expression of epithelial markers, and increased their expression of mesenchymal markers progressively with time. The cells also adopted a spindle-shaped morphology and cell-to-cell contact dependence was progressively lost, suggesting time-dependent occurrence of epithelial-mesenchymal transition. Furthermore, mRNA levels of CD44, a cancer stem cell marker, were time-dependently upregulated. When SCC cells were stimulated with Fusobacterium nucleatum for four weeks in the presence of dexamethasone, Fusobacterium nucleatum induced epithelial-mesenchymal transition was inhibited.

Conclusions

Epithelial-mesenchymal transition in human tongue SCC cells was time-dependently induced by prolonged, persistent Fusobacterium nucleatum stimulation and inhibited by dexamethasone. Routine decontamination of the oral cavity may be crucial for controlling tumor invasion and metastasis.

目的：有几项研究报告了核酸镰刀菌刺激对口腔癌细胞的影响。然而，鉴于这些研究的刺激期通常为 3 天至 8 天，迄今为止进行的体外研究可能无法完全模拟口腔癌环境，因为口腔癌环境涉及持续暴露于口腔共生细菌。本研究旨在阐明长时间持续感染核酸镰刀菌对口腔癌细胞的影响。方法：用核酸镰刀菌持续刺激人舌鳞状细胞癌（SCC）细胞两周或四周，然后进行实验评估：结果：随着时间的推移，长时间持续的核酸镰刀菌刺激增加了细胞的增殖、侵袭和迁移能力，降低了细胞上皮标志物的表达，增加了细胞间质标志物的表达。细胞还呈现纺锤形形态，细胞间的接触依赖性逐渐丧失，这表明上皮-间质转化的发生与时间有关。此外，癌症干细胞标记物 CD44 的 mRNA 水平也随时间而上调。在地塞米松存在的情况下，用核分枝杆菌刺激SCC细胞四周，核分枝杆菌诱导的上皮-间质转化受到抑制：结论：人舌SCC细胞的上皮-间质转化与核酸镰刀菌长时间、持续刺激的诱导和地塞米松的抑制有时间依赖性。口腔的常规净化可能是控制肿瘤侵袭和转移的关键。

{"title":"Epithelial-mesenchymal transition in oral cancer cells induced by prolonged and persistent Fusobacterium nucleatum stimulation","authors":"","doi":"10.1016/j.job.2024.05.006","DOIUrl":"10.1016/j.job.2024.05.006","url":null,"abstract":"<div><h3>Objectives</h3><p>Several studies have reported the effects of <span><span>Fusobacterium nucleatum</span></span><span> stimulation on oral cancer cells. However, given that these studies typically span a stimulation period of three days to eight days, the </span><em>in vitro</em> studies conducted to date may not fully mimic the oral cancer environment, which involves constant exposure to oral commensal bacteria. This study aimed to elucidate the effects of prolonged and persistent <em>Fusobacterium nucleatum</em> infection on oral cancer cells.</p></div><div><h3>Methods</h3><p><span>Human tongue squamous cell carcinoma (SCC) cells were continuously stimulated with </span><em>Fusobacterium nucleatum</em> for two or four weeks, then experimentally evaluated.</p></div><div><h3>Results</h3><p>Prolonged, persistent <em>Fusobacterium nucleatum</em><span> stimulation increased the cells’ proliferative, invasive, and migratory capacities, decreased their expression of epithelial markers, and increased their expression of mesenchymal markers progressively with time. The cells also adopted a spindle-shaped morphology and cell-to-cell contact dependence was progressively lost, suggesting time-dependent occurrence of epithelial-mesenchymal transition. Furthermore, mRNA levels of CD44, a cancer stem cell marker, were time-dependently upregulated. When SCC cells were stimulated with </span><em>Fusobacterium nucleatum</em><span> for four weeks in the presence of dexamethasone, </span><em>Fusobacterium nucleatum</em> induced epithelial-mesenchymal transition was inhibited.</p></div><div><h3>Conclusions</h3><p>Epithelial-mesenchymal transition in human tongue SCC cells was time-dependently induced by prolonged, persistent <em>Fusobacterium nucleatum</em><span><span> stimulation and inhibited by dexamethasone. Routine decontamination of the </span>oral cavity may be crucial for controlling tumor invasion and metastasis.</span></p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 3","pages":"Pages 594-604"},"PeriodicalIF":2.6,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Periostin regulates integrin expression in gingival epithelial cells 牙周蛋白调节牙龈上皮细胞中整合素的表达。

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-03-01 DOI: 10.1016/j.job.2023.11.009

Reika Hirata , Tomoyuki Iwata , Tsuyoshi Fujita , Takayoshi Nagahara , Shinji Matsuda , Shinya Sasaki , Yuri Taniguchi , Yuta Hamamoto , Kazuhisa Ouhara , Yasusei Kudo , Hidemi Kurihara , Noriyoshi Mizuno

Objective

Human gingival epithelial cells (HGECs) function as a mechanical barrier against invasion by pathogenic organisms through epithelial cell–cell junction complexes, which are complex components of integrin. Integrins play an important role in the protective functions of HGECs. Human periodontal ligament (HPL) cells regulate periodontal homeostasis. However, periodontitis results in the loss of HPL cells. Therefore, as replenishment, HPL cells or mesenchymal stem cells (MSCs) can be transplanted. Herein, HPL cells and MSCs were used to elucidate the regulatory mechanisms of HGECs, assuming periodontal tissue homeostasis.

Methods

Human gingival fibroblasts (HGFs), HGECs, HPL cells, and MSCs were cultured, and the conditioned medium was collected. With or without silencing periostin mRNA, HGECs were cultured under normal conditions or in a conditioned medium. Integrin and periostin mRNA expression was determined using real-time polymerase chain reaction. Integrin protein expression was analyzed using flow cytometry, and periostin protein expression was determined via western blotting.

Results

The conditioned medium affected integrin expression in HGECs. Higher expression of periostin was observed in MSCs and HPL cells than in HGFs. The conditioned medium that contained periostin protein regulated integrin expression in HGECs. After silencing periostin in MSCs and HPL cells, periostin protein was not detected in the conditioned medium, and integrin expression in HGECs remained unaffected.

Conclusions

Integrins in HGECs are regulated by periostin secreted from HPL cells and MSCs. This result suggests that periostin maintains gingival cell adhesion and regulates bacterial invasion/infection. Therefore, the functional regulation of periostin-secreting cells is important in preventing periodontitis.

目的:人牙龈上皮细胞(HGECs)通过上皮细胞-细胞连接复合物(整合素的复杂组分)作为抵抗病原生物入侵的机械屏障。整合素在hgec的保护功能中发挥重要作用。人牙周韧带(HPL)细胞调节牙周稳态。然而，牙周炎导致HPL细胞的损失。因此，作为补充，HPL细胞或间充质干细胞(MSCs)可以移植。本研究假设牙周组织稳态，利用HPL细胞和MSCs来阐明hgcs的调控机制。方法:培养人牙龈成纤维细胞(HGFs)、hgcs、HPL细胞和MSCs，并收集条件培养基。在正常条件下或条件培养基中，分别沉默或不沉默periostin mRNA培养hgcs。实时聚合酶链反应检测整合素和骨膜蛋白mRNA表达。流式细胞术检测整合素蛋白表达，western blotting检测骨膜蛋白表达。结果:条件培养基影响hgec中整合素的表达。骨膜蛋白在MSCs和HPL细胞中的表达高于HGFs。含periostin蛋白的条件培养基可调节hgec中整合素的表达。在MSCs和HPL细胞中沉默骨膜蛋白后，在条件培养基中检测不到骨膜蛋白，hgcs中整合素的表达不受影响。结论:hgcs中的整合素受HPL细胞和MSCs分泌的骨膜蛋白的调控。结果表明，牙周素具有维持牙龈细胞粘附和调节细菌侵袭/感染的作用。因此，分泌牙周炎细胞的功能调控在预防牙周炎中具有重要意义。

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引用次数: 0

A quantitative study of the development of taste pores in mice 小鼠味孔发育的定量研究。

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-03-01 DOI: 10.1016/j.job.2024.01.013

Atsuko Yamashita, Masato S. Ota

Objectives

This study determined the early development of taste buds by observing the changes in the three-dimensional structures of taste pores and microvilli in the circumvallate papillae (CVP) of mice, from pre- and postnatal stages to the adult stages.

Methods

Fragments of mouse CVP tissue were collected on embryonic day (E) 18 and postnatal days (P) 0, 3, 6, 7, 14, 21, 28, and 56. The surfaces of the tissue fragments located pore apertures via scanning electron microscopy, and the sizes of the CVP and maximum diameters of the pores were estimated from the recorded images. Likewise, changes in the structures of the epithelium around the pore aperture and microvilli protruding from the pores were examined.

Results

The size of the CVP exhibited a linear increase with age from E18 to P56. The epithelium around the pore aperture demonstrated changes to form microridges, indicating a characteristic pattern during CVP development. The size of the pore aperture also increased with age from E18 to P56. Furthermore, an increase in the number of pores with protruding microvilli was observed at the base of the epithelial trench. A significant positive correlation was observed between the maximum diameter of the pore and the size of the CVP.

Conclusions

The expansion in the lateral view of the CVP was associated with the developmental stage from E18 to P56, suggesting that the growth of the CVP leads to the opening and enlargement of the taste pores with microvillus projections during these stages.

研究目的本研究通过观察小鼠周壁乳头（CVP）味孔和微绒毛三维结构从出生前、出生后到成年阶段的变化，确定味蕾的早期发育过程：方法：在胚胎第（E）18天和出生后第（P）0、3、6、7、14、21、28和56天采集小鼠CVP组织片段。通过扫描电子显微镜对组织碎片表面的孔隙进行定位，并根据记录的图像估算 CVP 的大小和孔隙的最大直径。同样，还检测了孔径周围上皮细胞结构的变化以及从孔隙中伸出的微绒毛：结果：从 E18 到 P56，CVP 的大小随着年龄的增长呈线性增长。孔径周围的上皮细胞发生了变化，形成了微嵴，这表明了CVP发育过程中的特征模式。从 E18 到 P56，孔径的大小也随着年龄的增长而增加。此外，在上皮沟槽基部还观察到带有突出微绒毛的孔数量增加。孔隙的最大直径与 CVP 的大小呈明显的正相关：结论：CVP侧视图的扩大与E18至P56的发育阶段有关，这表明在这些阶段，CVP的生长会导致带有微绒毛突起的味孔的打开和扩大。

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引用次数: 0

Comparison between hangeshashinto and dexamethasone for IL-1α and β-defensin 1 production by human oral keratinocytes 比较恒沙欣多和地塞米松对人类口腔角质细胞产生 IL-1α 和 β-defensin 1 的作用。

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-03-01 DOI: 10.1016/j.job.2024.01.007

Hiroyuki Hato , Atsushi Kaneko , Chiho Maeda , Ken-ichiro Sakata , Yusuke Ono , Yusuke Mizukami , Toru Kono , Yoshimasa Kitagawa

Objective

Human β-defensin 1 (hBD-1) is a antimicrobial peptide that is constantly secreted by oral tissues. Hangeshashinto (HST), a traditional Japanese medicine, has been reported to be effective against stomatitis. This study aimed to clarify the profile of HST by comparing the system of production of interleukin-1α (IL-1α) and hBD-1 in human oral mucosal epithelial cells with dexamethasone (DEX), a steroid used for the treatment of stomatitis.

Methods

Human oral keratinocytes (HOK) were treated with HST, DEX, or HST components (baicalein, baicalin, berberine, and glycyrrhizin) for 24 h, and subsequently cultured for 24 h with or without Pam3CSK4 or lipopolysaccharide (LPS). The cell supernatants, total RNA, and intracellular proteins were collected, and changes in IL-1α and hBD-1 protein production and gene expression were evaluated using ELISA and RT-PCR. The phosphorylation of NF-kB and the cell proliferative ability of HOK were evaluated by western blotting and XTT assay, respectively.

Results

DEX (0.01–10 μM) significantly suppressed IL-1α and hBD-1 production induced by either Pam3CSK4 or LPS, and also decreased cell growth. In contrast, HST inhibited Pam3CSK4- and LPS-induced IL-1α production at a concentration range of 12.5–100 μg/mL without affecting the cell proliferative capacity and hBD-1 production of HOK. Baicalein and baicalin, which are flavonoid ingredients of HST, showed anti-IL-1α production.

Conclusion

HST may be useful as a therapeutic agent for stomatitis and other inflammatory diseases of the oral cavity.

目的：人 β防御素 1（hBD-1）是口腔组织不断分泌的一种抗菌肽。据报道，日本传统药物 Hangeshashinto（HST）对口腔炎有效。本研究旨在通过比较白细胞介素-1α（IL-1α）和 hBD-1 在人类口腔黏膜上皮细胞中的分泌系统与地塞米松（DEX）（一种用于治疗口腔炎的类固醇），来阐明 HST 的特性。方法：用 HST、DEX 或 HST 成分（黄芩素、黄芩苷、小檗碱和甘草甜素）处理人口腔角质细胞（HOK）24 小时，然后用或不用 Pam3CSK4 或脂多糖（LPS）培养 24 小时。收集细胞上清、总 RNA 和细胞内蛋白，并用 ELISA 和 RT-PCR 评估 IL-1α 和 hBD-1 蛋白生成和基因表达的变化。结果显示：DEX（0.01-10 μg/L）和HOK（0.01-10 μg/L）对HOK细胞增殖能力和NF-kB磷酸化均有影响：结果：DEX（0.01-10 μM）能明显抑制 Pam3CSK4 或 LPS 诱导的 IL-1α 和 hBD-1 的产生，并能降低细胞的生长。相反，在 12.5-100 μg/mL 的浓度范围内，HST 可抑制 Pam3CSK4 和 LPS 诱导的 IL-1α 的产生，但不影响 HOK 的细胞增殖能力和 hBD-1 的产生。黄芩中的黄芩素和黄芩苷具有抗 IL-1α 的作用：结论：黄芩苷和黄芩素可以作为口腔炎和其他口腔炎症的治疗药物。

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引用次数: 0

Medicinal herbs, especially Hibiscus sabdariffa, inhibit oral pathogenic bacteria 药用草本植物，尤其是木槿，可抑制口腔致病菌。

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-03-01 DOI: 10.1016/j.job.2024.01.006

Kazuya Takada , Shizuki Nakano , Reina Nishio , Daichi Muku , Shinichi Mochizuki , Inori Inui , Kaede Okita , Ayaka Koga , Koji Watanabe , Yoshie Yoshioka , Wataru Ariyoshi , Ryota Yamasaki

Objectives

Medicinal herbs are plants with potential medicinal and health benefits. In recent years, they are being increasingly used as a treatment alternative owing to their effectiveness against various diseases. In this study, we investigated the inhibitory effects of 15 medicinal herbs on causative bacteria for dental caries and periodontal disease.

Methods

This study evaluated the effects of the extracts of 15 medicinal herbs on growth and biofilm formation in five oral pathogenic bacterial strains. The herbs were processed into extracts, and bacterial strains were cultured. Then, bacterial growth and biofilm formation were assessed using various methods. Finally, the extract of the herb Hibiscus sabdariffa (hibiscus) was analyzed using high-performance liquid chromatography.

Results

Incubation of bacteria with the herbal extracts showed that hibiscus exerted a significant inhibitory effect on all the oral pathogenic bacterial strains evaluated in this study. In addition, the pigment delphinidin-3-sambubioside, which is found in hibiscus extract, was identified as a particularly important inhibitory component.

Conclusions

These results lay the ground work for the potential development of novel therapeutic or preventive agents against dental caries and periodontal disease, two major oral diseases.

目的：药草是具有潜在药用和保健功效的植物。近年来，由于药草对各种疾病的疗效显著，越来越多的人将其作为一种替代治疗方法。本研究调查了 15 种药草对龋齿和牙周病致病菌的抑制作用：本研究评估了 15 种药草提取物对五种口腔致病菌株的生长和生物膜形成的影响。将草药加工成提取物，培养细菌菌株。然后，使用各种方法评估细菌的生长和生物膜的形成。最后，使用高效液相色谱法分析了木槿（Hibiscus sabdariffa）的提取物：结果：用草药提取物培养细菌的结果表明，木槿对本研究中评估的所有口腔致病细菌菌株都有明显的抑制作用。此外，木槿提取物中的色素delphinidin-3-sambubioside被鉴定为一种特别重要的抑制成分：这些结果为开发新型治疗或预防龋齿和牙周病这两种主要口腔疾病的药物奠定了基础。

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