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Effects of microabrasion and bleaching on color and shear bond strength of simulated stained-remineralized caries lesions 微磨蚀和漂白对模拟染色再矿化龋损颜色和剪切粘接强度的影响
IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-12 DOI: 10.1016/j.sdentj.2024.06.014

Purpose

This study investigated the effects of bleaching and microabrasion on the color and shear bond strength (SBS) of stained-remineralized caries-like lesions (s-RCLs).

Methods

Human enamel specimens were demineralized, stained (coffee/tea), then remineralized (2% NaF and artificial saliva [AS]) to create s-RCLs. Specimens were randomly divided into five groups (n = 18): G1, demineralized/AS; G2, s-RCLs/AS; G3, s-RCLs/at-home bleaching (15% carbamide peroxide [CP)], 6 h/d×7)/AS; G4, s-RCLs/microabrasion (6.6% hydrochloric acid, [1min/3cycles]/AS; and G5, s-RCLs/microabrasion and at-home bleaching/AS. Color was spectrophotometrically measured at baseline, after demineralization, after staining, and after treatment. After two weeks, the SBS was tested using a universal testing machine. Outcomes were analyzed using ANOVA models followed by Tukey’s test (α = 0.05).

Results

The mean colors (ΔE) for demineralization and staining were significant (ΔE ≤ 5.9 and ≤14.4, respectively). G3 (ΔE 23.9) and G5 (ΔE 25.2) were significantly improved compared to G4 (ΔE 12.3). The SBS in G5 had the highest significant value (25.2 MPa), followed by G4 and G3 (21.5–20.6 MPa), which were significantly higher than G2 (16.8 MPa). G2, in turn, was more significant than G1 (10.9 MPa).

Conclusion

At-home bleaching (15% CP) for seven days eliminated and improved organic stains on RCLs. Faster results were achieved when combined with microabrasion. All surface treatments resulted in high SBS.

目的 本研究探讨了漂白和微磨蚀对染色再矿化龋样病变(s-RCLs)的颜色和剪切粘结强度(SBS)的影响。方法 将人类珐琅质标本脱矿、染色(咖啡/茶),然后再矿化(2% NaF 和人工唾液 [AS]),形成 s-RCLs。标本被随机分为五组(n = 18):G1,脱矿/AS;G2,s-RCLs/AS;G3,s-RCLs/居家漂白(15%过氧化卡酰胺[CP],6小时/d×7)/AS;G4,s-RCLs/微磨(6.6%盐酸,[1分钟/3周期]/AS;G5,s-RCLs/微磨和居家漂白/AS。分别在基线、脱矿后、染色后和治疗后用分光光度法测量颜色。两周后,使用万能试验机对 SBS 进行测试。结果脱矿和染色的平均颜色(ΔE)显著(ΔE 分别≤5.9 和≤14.4)。与 G4(ΔE 12.3)相比,G3(ΔE 23.9)和 G5(ΔE 25.2)有明显改善。G5 的 SBS 显著值最高(25.2 兆帕),其次是 G4 和 G3(21.5-20.6 兆帕),明显高于 G2(16.8 兆帕)。结论持续七天的家庭漂白(15% CP)可消除和改善 RCL 上的有机污渍。如果结合微磨,效果会更快。所有的表面处理都能产生较高的 SBS。
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引用次数: 0
Fracture Resistance in Fibre-Reinforced Resin Composite Restorations in Deciduous and Permanent Molars: An Ex Vivo Study 纤维增强树脂复合修复体在落牙和恒磨牙中的抗折性:体外研究
IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-12 DOI: 10.1016/j.sdentj.2024.06.017

Aim

The aim of this study was to compare the fracture resistance levels of restored deciduous teeth and permanent molars restored with different materials, including ultra-polyethylene fibre tape (Ribbond-Ultra), fibre-reinforced resin composite EverX posterior, fibre-reinforced flowable resin composite EverX Flow and bulk-fill flow restorative material (Tetric N-flow) in the posterior region.

Methods

We tested sixty-four caries-free human mandibular molars (32 deciduous molars and 32 permanent molars). Deep and wide Class I cavities were prepared in each tooth. The teeth were embedded in poly(methyl methacrylate) resin up to the cementoenamel junction, leaving the crown completely exposed. Oral temperature fluctuations were then simulated using two thousand thermocycling cycles, each lasting 30 seconds in the hot phase (approximately 35 °C). The loading rate for our force-fracture tests was set at 0.5 mm/min. Direct restorations were made using a spherical tip and applying the force perpendicular to the sample surface. Visual inspection of the fractured specimens, in combination with adhesive scanning electron microscopy (SEM) and finite element analysis (FEA), provided detailed insights into the failure modes and stress distribution at the restoration–tooth interface.

Results

Teeth restored with fibre-reinforced composite (EverX Posterior) had the highest fracture resistance, followed by fibre-reinforced flowable resin composite (EverX Flow). Teeth restored with the ultra- polyethylene fibre tape (Ribbond-Ultra), followed by the flowable bulk-fill composite (Tetric N-flow) had the lowest resistance. Statistical analysis revealed significant differences between the groups, except for EverX Posterior and EverX Flow. A predictive algorithm was also proposed for the likelihood of restoration failure.

Conclusion

Modern fibre-reinforced resin composites, such as EverX Posterior, effectively reinforce teeth against fractures, with success in both restored deciduous and permanent molars. Meanwhile, the use of polyethylene fibre tapes is less effective, and they involve a  time-consuming application.

本研究的目的是比较使用不同材料(包括超聚乙烯纤维带(Ribbond-Ultra)、纤维增强型树脂复合材料 EverX 后牙、纤维增强型流动树脂复合材料 EverX Flow 和后牙区域的大量填充流动修复材料(Tetric N-flow))修复的乳牙和恒磨牙的抗折性水平。在每颗牙齿上都制备了深而宽的 I 级龋洞。将牙齿嵌入聚甲基丙烯酸甲酯树脂中,直至牙本质釉质交界处,使牙冠完全暴露。然后使用两千个热循环周期模拟口腔温度波动,每个热循环周期持续 30 秒(约 35 °C)。力-断裂测试的加载速度设定为 0.5 毫米/分钟。使用球形针尖进行直接修复,施加的力垂直于试样表面。通过对断裂试样进行目视检查,并结合粘接扫描电子显微镜(SEM)和有限元分析(FEA),可以详细了解修复体与牙齿界面的断裂模式和应力分布情况。结果使用纤维增强复合材料(EverX Posterior)修复的牙齿具有最高的抗断裂性,其次是纤维增强可流动树脂复合材料(EverX Flow)。用超聚乙烯纤维带(Ribbond-Ultra)修复的牙齿抗折性最低,其次是可流动的散装填充复合材料(Tetric N-flow)。统计分析显示,除 EverX Posterior 和 EverX Flow 外,各组之间存在明显差异。结论EverX Posterior 等现代纤维增强树脂复合材料能有效加固牙齿,防止牙齿断裂,在修复乳磨牙和恒磨牙方面都取得了成功。与此同时,使用聚乙烯纤维带的效果较差,而且使用起来比较费时。
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引用次数: 0
Aesthetic problems related to dental implants in the aesthetic zone: A systematic review 与美学区种植牙相关的美学问题:系统回顾
IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-03 DOI: 10.1016/j.sdentj.2024.06.010

Background

Patient satisfaction is a crucial indicator of a successful dental implant treatment. Attainment of an aesthetically acceptable final result is central to this success. Hence, the primary objective of this systematic review was to identify aesthetic concerns linked to dental implants within the aesthetic zone.

Methods

An electronic search was conducted on different databases such as PubMed, Medline, and Google Scholar using the key terms “tooth implant,” “anterior implant,” “dental implantation in the aesthetic zone,” and “aesthetic area implant. Following the elimination of duplicate articles and adherence to the inclusion criteria, 14 articles (five randomized clinical trials, four prospective clinical trials, four case series, and one pilot study) published between 2007 and 2020 were deemed suitable for this review.

Results

The selected studies highlighted several aesthetic issues, such as recession, marginal bone loss, gingival loss, and pink aesthetic scores related to dental implants placed in the aesthetic zone.

Conclusion

Addressing these challenges necessitates meticulous treatment planning, effective soft tissue management, regular follow-ups, patient education, expectation management, and collaborative efforts among specialists. A limitation of this systematic review is that it focused solely on studies conducted within a relatively short timeframe (2007–2020). However, it is essential to recognize that significant changes occurred in the treatment procedures and materials within the field during this period. These changes have resulted in notable variances in the treatment protocols utilized across studies, potentially affecting the generalizability of the findings.

背景患者满意度是衡量种植牙治疗成功与否的重要指标。获得美学上可接受的最终结果是成功的关键。因此,本系统性综述的主要目的是确定与美学区内牙科植入物相关的美学问题。方法使用关键术语 "牙科植入物"、"前部植入物"、"美学区内的牙科植入物 "和 "美学区植入物 "在 PubMed、Medline 和 Google Scholar 等不同数据库中进行了电子检索。在剔除重复文章并遵守纳入标准后,2007 年至 2020 年间发表的 14 篇文章(5 篇随机临床试验、4 篇前瞻性临床试验、4 篇病例系列和 1 篇试点研究)被认为适合纳入本综述。结果所选研究强调了几个美学问题,如衰退、边缘骨丧失、牙龈丧失,以及与在美学区植入牙科种植体相关的粉色美学评分。本系统综述的局限性在于,它只关注了相对较短时间内(2007-2020 年)进行的研究。然而,我们必须认识到,在此期间,该领域的治疗程序和材料发生了重大变化。这些变化导致不同研究采用的治疗方案存在明显差异,可能会影响研究结果的可推广性。
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引用次数: 0
The Development and Utilization of an Electronic Assessment Software in Dental Education: A Comprehensive Evaluation 牙科教育中电子评估软件的开发和使用:综合评估
IF 1.8 Q2 Dentistry Pub Date : 2024-05-26 DOI: 10.1016/j.sdentj.2024.05.010
Ali Al Ehaideb , Abdulmohsen Alfadley , Maryam A. Alghilan , Fathima F Farook , Nora Alhazmi , Elgene Castaneda , Fatimah Al Qarni , Muhammad Nadeem , Lubna Alkadi

Purpose/Objectives

This article introduces the Key Electronic Assessment Platform (KEAP), an electronic-based assessment platform created in-house at the College of Dentistry (COD), King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Riyadh, Saudi Arabia. It highlights the platform’s integral value and demand within the educational system.

Methods

The article introduces the KEAP system design and architecture, describes its features, tests, and implementation in addition to its maintenance and security. It also presents the outcomes of KEAP utilization and the level of users’ satisfaction.

Results

The KEAP item bank has been expanded by more than 26,500 questions over the last four academic years and 1,255 exams have been administered. KEAP provides a highly secure structured framework for assessment planning, conduction, and evaluation. More than 90% of students and faculty are satisfied or very satisfied with their experience using the KEAP system indicating that it is well accepted by the end users.

Conclusions

The development of KEAP is based on contextual factors in COD, KSAU-HS with the aim of creating a process for assessment that is valid, efficient, standardized, and highly secure. KEAP’s design and successful implementation can provide a successful example of implementing electronic-based assessment in higher education.

目的/目标本文介绍了关键电子评估平台(KEAP),这是沙特阿拉伯利雅得沙特本阿卜杜勒阿齐兹国王健康科学大学(KSAU-HS)牙科学院(COD)内部创建的电子评估平台。文章介绍了 KEAP 系统的设计和架构,描述了其功能、测试和实施,以及其维护和安全性。结果在过去的四个学年中,KEAP题库已经扩充了26500多道题目,并进行了1255次考试。KEAP 为评估规划、实施和评估提供了一个高度安全的结构化框架。超过 90% 的学生和教师对 KEAP 系统的使用体验表示满意或非常满意,这表明该系统得到了最终用户的广泛认可。KEAP 的设计和成功实施为在高等教育中实施电子评估提供了一个成功的范例。
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引用次数: 0
Health status and visit reasons for children attending the Pediatric Dentistry department in damascus university, damascus, Syria: A retrospective study 叙利亚大马士革大学儿童牙科就诊儿童的健康状况和就诊原因:回顾性研究
IF 1.8 Q2 Dentistry Pub Date : 2024-05-20 DOI: 10.1016/j.sdentj.2024.05.009
Mohamad Bashier Almonaqel , Ramah Eimad Makieh

Objectives

This study aimed to discuss the health status and treatment requirements of children visiting the Department of Pediatric Dentistry at the Faculty of Dentistry.

Materials and Methods

A retrospective study included the records of patients attending the Department of Pediatric Dentistry, from November 29, 2021, to December 14, 2023.

Results

The study reviewed 2417 patient records, excluding 312. The majority of patients were primary school children aged 6 − <12, primarily from Rif-Dimashq governorate. About 18 % displayed negative behavior during examinations, and 8 % had special health care needs. The most frequent type of disability was medical disabilities, followed by neurological disorders, with percentages of 40.2 % and 28.9 %, respectively. Dental caries were the primary reason for visits, with a mean of 3.94 ± 3.13. A negative association was found between the number of decayed teeth and the following: age classification, visit year, and Salviov-Tinawi scale. The percentage of males who came for cosmetic problems was higher than females at 57.2 % and 42.8 %, respectively.

Conclusions

The Department of Pediatric Dentistry at the Faculty of Dentistry, Damascus University, is a public dental clinic that is visited by patients from all governorates, providing free specialized treatments.

Clinical relevance

Unmet dental needs are a significant issue for both healthy children and those with special health care needs. The Department of Pediatric Dentistry serves as a research and educational center, offering comprehensive dental treatments to children from all socioeconomic backgrounds. Therefore, understanding the dental needs of children is crucial for providing effective support.

材料和方法回顾性研究包括 2021 年 11 月 29 日至 2023 年 12 月 14 日期间在儿童牙科就诊的患者记录。结果研究审查了 2417 份患者记录,排除了 312 份。大多数患者是 6 - 12 岁的小学生,主要来自里夫-迪马士格省。约 18% 的患者在检查过程中表现出消极行为,8% 的患者有特殊医疗需求。最常见的残疾类型是内科残疾,其次是神经系统疾病,比例分别为 40.2% 和 28.9%。龋齿是就诊的主要原因,平均为 3.94 ± 3.13。龋齿数量与以下因素呈负相关:年龄分类、就诊年份和 Salviov-Tinawi 评分。结论大马士革大学牙科学院儿童牙科系是一家公立牙科诊所,为来自各省的患者提供免费的专业治疗。临床相关性对于健康儿童和有特殊医疗保健需求的儿童来说,满足他们的牙科需求都是一个重要问题。儿童牙科部是一个研究和教育中心,为来自各种社会经济背景的儿童提供全面的牙科治疗。因此,了解儿童的牙科需求对于提供有效的支持至关重要。
{"title":"Health status and visit reasons for children attending the Pediatric Dentistry department in damascus university, damascus, Syria: A retrospective study","authors":"Mohamad Bashier Almonaqel ,&nbsp;Ramah Eimad Makieh","doi":"10.1016/j.sdentj.2024.05.009","DOIUrl":"10.1016/j.sdentj.2024.05.009","url":null,"abstract":"<div><h3>Objectives</h3><p>This study aimed to discuss the health status and treatment requirements of children visiting the Department of Pediatric Dentistry at the Faculty of Dentistry.</p></div><div><h3>Materials and Methods</h3><p>A retrospective study included the records of patients attending the Department of Pediatric Dentistry, from November 29, 2021, to December 14, 2023.</p></div><div><h3>Results</h3><p>The study reviewed 2417 patient records, excluding 312. The majority of patients were primary school children aged 6 − &lt;12, primarily from Rif-Dimashq governorate. About 18 % displayed negative behavior during examinations, and 8 % had special health care needs. The most frequent type of disability was medical disabilities, followed by neurological disorders, with percentages of 40.2 % and 28.9 %, respectively. Dental caries were the primary reason for visits, with a mean of 3.94 ± 3.13. A negative association was found between the number of decayed teeth and the following: age classification, visit year, and Salviov-Tinawi scale. The percentage of males who came for cosmetic problems was higher than females at 57.2 % and 42.8 %, respectively.</p></div><div><h3>Conclusions</h3><p>The Department of Pediatric Dentistry at the Faculty of Dentistry, Damascus University, is a public dental clinic that is visited by patients from all governorates, providing free specialized treatments.</p></div><div><h3>Clinical relevance</h3><p>Unmet dental needs are a significant issue for both healthy children and those with special health care needs. The Department of Pediatric Dentistry serves as a research and educational center, offering comprehensive dental treatments to children from all socioeconomic backgrounds. Therefore, understanding the dental needs of children is crucial for providing effective support.</p></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S101390522400155X/pdfft?md5=1c31923d29e75b261863513de92c978a&pid=1-s2.0-S101390522400155X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141130454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The association between oral health literacy and oral health-related behaviours among female adolescents in the Kingdom of Saudi Arabia: A cross-sectional study 沙特阿拉伯王国女性青少年口腔健康知识与口腔健康相关行为之间的关系:横断面研究
IF 1.8 Q2 Dentistry Pub Date : 2024-05-18 DOI: 10.1016/j.sdentj.2024.05.007
Muneera Alzeer , AlBandary AlJameel , Kasper Rosing , Esben Øzhayat

Objectives

Oral health literacy (OHL) is suggested as an important denominator of oral health. This study aimed to identify ways to improve oral health by exploring the association between oral health literacy and oral health-related behaviours among female adolescents in the Eastern Province of the Kingdom of Saudi Arabia (KSA).

Methods

This cross-sectional study assessed OHL among 1,889 10th grade female students in the Eastern Province of the KSA. OHL was assessed using an Arabic version of the short version of the Health Literacy in Dentistry scale (A-HeLD-14). Self-reported socio-demographic information and oral health-related behaviours (toothbrushing, dental attendance, and sugary diet consumption) were also collected. The relationship between OHL and oral health-related behaviours was investigated using binary logistic regression, adjusted for socio-demographic information.

Results

The binary logistic regression analyses showed that poor OHL was significantly associated with infrequent toothbrushing (p < 0.001) and irregular dental attendance (p = 0.005) but not with consumption of sugary diets. All A-HeLD-14 domains were significantly associated with infrequent toothbrushing, and the domains concerning access, receptivity, and financial barriers were significantly associated with irregular dental attendance. The highest odds ratios (ORs) for infrequent toothbrushing were found in the domains of receptivity (OR = 4.19) and understanding (OR = 3.85) and for irregular dental attendance in the financial barriers (OR = 1.61) and access (OR = 1.49) domains, followed by the receptivity domain (OR = 1.35).

Conclusion

Poor OHL was significantly associated with infrequent toothbrushing and irregular dental attendance. Interventions focusing on OHL are warranted to improve oral health-related behaviours and thereby oral health in the population. The aspects that seem most vital to target are improved access to dental care, including the reduction of economic barriers, as well as increased awareness of oral health in the population.

目标口腔健康素养(OHL)被认为是口腔健康的重要标准。本研究旨在通过探索沙特阿拉伯王国(KSA)东部省女性青少年的口腔健康素养与口腔健康相关行为之间的关联,找出改善口腔健康的方法。方法 本横断面研究评估了沙特阿拉伯东部省 1889 名十年级女生的口腔健康素养。OHL 采用阿拉伯语版的牙科健康素养简易量表(A-HeLD-14)进行评估。此外,还收集了自我报告的社会人口信息和口腔健康相关行为(刷牙、看牙医和食用含糖饮食)。结果二元逻辑回归分析表明,口腔健康欠佳与不经常刷牙(p < 0.001)和不定期看牙(p = 0.005)显著相关,但与含糖饮食的摄入量无关。A-HeLD-14的所有领域都与不经常刷牙显著相关,而有关获取、接受和经济障碍的领域则与不经常看牙医显著相关。在接受能力(OR = 4.19)和理解能力(OR = 3.85)领域,不经常刷牙的几率比(OR)最高;在经济障碍(OR = 1.61)和获取能力(OR = 1.49)领域,不经常看牙医的几率比(OR)最高,其次是接受能力领域(OR = 1.35)。为了改善口腔健康相关行为,进而改善人口的口腔健康状况,有必要采取以口腔健康相关行为为重点的干预措施。最重要的目标是改善获得牙科保健的途径,包括减少经济障碍,以及提高人们对口腔健康的认识。
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引用次数: 0
Benign fibro-osseous lesions: A retrospective study of sixty-four cases from a single institute, Riyadh, Saudi Arabia 良性纤维骨病变:对沙特阿拉伯利雅得一家研究所六十四例病例的回顾性研究
IF 1.8 Q2 Dentistry Pub Date : 2024-05-11 DOI: 10.1016/j.sdentj.2024.05.005
Asma Almazyad , Adwaa Alhumaidan , Manal AlSheddi

Objective

Benign fibroosseous lesions (BFOLs) encompass a heterogenous collection of bone conditions characterized by replacing normal bone with fibro-collagenous tissue with osteoid or woven bone, and cementicles. Despite their clinical significance, the frequency of BFOLs in Saudi Arabia still needs to be assessed.

Methods

This retrospective study investigated the frequency and demographics of BFOLs in Riyadh, Saudi Arabia, by retrieving all cases recorded between January 1984 and January 2013 from a single Oral Pathology Laboratory archive.

Results

A total of 64 cases were classified as BFOLs, with a predominance in females (67.2 %) and a median age of 21.5 years. The most prevalent condition identified was fibrous dysplasia (45.31 %), followed by cemento-ossifying fibroma (26.56 %). There were significant sex differences between BFOLs, with a p-value of 0.03. FD was predominantly located in the maxilla (65.5 %), whereas COF was predominantly found in the mandible (82.3 %). Recurrence was observed in 17.2 % of patients with FD, in contrast to no reported recurrence in patients with COF.

Conclusion

This study represents the first exploration of BFOL frequency and demographics in Riyadh, Saudi Arabia, highlighting the need for further investigations to comprehensively understand the nature of these lesions in our population.

目的良性骨纤维性病变(Benign fibroosseous lesions,BFOLs)包括一系列不同类型的骨病,其特点是用纤维胶原组织取代正常骨,并伴有骨质或编织骨和骨水泥柱。这项回顾性研究通过检索 1984 年 1 月至 2013 年 1 月期间从单一口腔病理实验室档案中记录的所有病例,调查了沙特阿拉伯利雅得 BFOL 的发病频率和人口统计学特征。最常见的病症是纤维发育不良(45.31%),其次是骨水泥化纤维瘤(26.56%)。BFOL之间存在明显的性别差异,P值为0.03。骨化性纤维瘤主要位于上颌骨(65.5%),而骨化性纤维瘤主要位于下颌骨(82.3%)。该研究首次探讨了沙特阿拉伯利雅得的 BFOL 频率和人口统计学特征,强调了进一步调查以全面了解我国人口中这些病变性质的必要性。
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引用次数: 0
Downregulation of connexin 43 is crucial for basal cell alignment in ameloblastoma and odontogenic keratocyst 连接蛋白 43 的下调对牙釉质母细胞瘤和牙源性角化囊肿的基底细胞排列至关重要
IF 1.8 Q2 Dentistry Pub Date : 2024-05-08 DOI: 10.1016/j.sdentj.2024.05.003
Ahmed Abdelaziz Mohamed Essa

Background

The current study aims at investigating gap junctions which allow cells to connect with one another. Such process is essential for cell differentiation and the preservation of diverse cell functions. It is noticeable that connexin 43 (Cnx43) was differentially expressed in ameloblasts and odontoblasts in the processes of odontogenesis. Moreover, in carcinoma in situ (CIS) and oral squamous cell carcinoma (SCC), Cnx43 expression apparently thought to be a defining feature of the neoplastic state of squamous epithelial cells. Aim: Therefore, the study has postulated that Cnx43 may be involved in the pathophysiology of ameloblastoma and certain odontogenic cysts whose epithelial constituents exhibit squamous cells.

Materials and methods

In order to prove the foregoing hypothesis, the study explored the immunohistochemical profiles of Cnx43 in ameloblastoma as well as some odontogenic cysts to assess Cnx43 trafficking and its relation with characteristic tissue architectures of odontogenic lesions. Results: The study has concluded that Cnx43 was down regulated significantly in follicular ameloblastoma with obvious ameloblasts-like cell components as well as in odontogenic keratocyst with palisaded basal cells. Additionally, other patterns of ameloblastoma (plexiform and desmoplastic) and different types of odontogenic cysts manifest heavy trafficking for Cnx43. Conclusion: Finally, altered Cnx43 expression between various patterns of ameloblastoma and odontogenic cysts might be related to their pathogenesis and is responsible for their morphological diversity.

背景 目前的研究旨在调查能让细胞相互连接的间隙连接。这一过程对于细胞分化和保持细胞的多种功能至关重要。值得注意的是,在牙胚形成过程中,连接蛋白 43(Cnx43)在牙釉质母细胞和牙本质母细胞中有不同程度的表达。此外,在原位癌(CIS)和口腔鳞状细胞癌(SCC)中,Cnx43 的表达显然被认为是鳞状上皮细胞肿瘤状态的一个决定性特征。目的:因此,本研究推测 Cnx43 可能参与了牙釉质母细胞瘤和某些牙源性囊肿的病理生理学,这些囊肿的上皮成分表现为鳞状细胞。材料与方法为了证明上述假设,本研究探讨了牙釉质母细胞瘤和某些牙源性囊肿中 Cnx43 的免疫组化特征,以评估 Cnx43 的贩运及其与牙源性病变的特征性组织结构之间的关系。结果:研究结果表明,在具有明显成釉细胞样细胞成分的滤泡性成釉细胞瘤以及具有基底细胞的牙源性角化囊肿中,Cnx43的调控能力明显下降。此外,其他类型的绒毛状母细胞瘤(丛状和脱屑性)和不同类型的牙源性囊肿也表现出 Cnx43 的大量贩运。结论最后,不同形态的釉母细胞瘤和牙源性囊肿的Cnx43表达改变可能与它们的发病机制有关,也是它们形态多样性的原因。
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引用次数: 0
Bioengineering from the laboratory to clinical translation in oral and maxillofacial reconstruction 口腔颌面重建中从实验室到临床转化的生物工程
IF 1.8 Q2 Dentistry Pub Date : 2024-05-08 DOI: 10.1016/j.sdentj.2024.05.004
Ola M. Maria , Ashraf Heram , Simon D. Tran

Background

Conventional techniques used in oral and maxillofacial reconstruction focus mainly on utilizing autologous tissues that have unquestionably improved function and esthetics for many patients, worldwide. However, the success depends on countless factors such as: donor and recipient sites conditions, patient’s medical history, surgeon’s experience, restricted availability of high-quality autogenous tissues or stem cells, and increased surgical cost and time.

Materials and Methods

Lately, teaming researchers, scientists, surgeons, and engineers, to address these limitations, have allowed tremendous progress in recombinant protein therapy, cell-based therapy, and gene therapy.

Results

Over the past few years, biomedical engineering has been evolving from the laboratory to clinical applications, for replacement of damaged body tissues due to trauma, cancer, congenital or acquired disorders.

Conclusions

This review provides an outlook on the content, benefits, recent advances, limitations, and future expectations of biomedical engineering for salivary glands, oral mucosa, dental structures, and maxillofacial reconstruction.

背景口腔颌面部重建中使用的传统技术主要侧重于利用自体组织,毫无疑问,自体组织改善了全球许多患者的功能和美观。然而,其成功与否取决于无数因素,例如:供体和受体部位的条件、患者的病史、外科医生的经验、高质量自体组织或干细胞的限制性供应以及手术成本和时间的增加。结果在过去几年中,生物医学工程已从实验室发展到临床应用,用于替代因创伤、癌症、先天性或后天性疾病而受损的身体组织。结论本综述对生物医学工程在唾液腺、口腔粘膜、牙科结构和颌面部重建方面的内容、益处、最新进展、局限性和未来预期进行了展望。
{"title":"Bioengineering from the laboratory to clinical translation in oral and maxillofacial reconstruction","authors":"Ola M. Maria ,&nbsp;Ashraf Heram ,&nbsp;Simon D. Tran","doi":"10.1016/j.sdentj.2024.05.004","DOIUrl":"10.1016/j.sdentj.2024.05.004","url":null,"abstract":"<div><h3>Background</h3><p>Conventional techniques used in oral and maxillofacial reconstruction focus mainly on utilizing autologous tissues that have unquestionably improved function and esthetics for many patients, worldwide. However, the success depends on countless factors such as: donor and recipient sites conditions, patient’s medical history, surgeon’s experience, restricted availability of high-quality autogenous tissues or stem cells, and increased surgical cost and time.</p></div><div><h3>Materials and Methods</h3><p>Lately, teaming researchers, scientists, surgeons, and engineers, to address these limitations, have allowed tremendous progress in recombinant protein therapy, cell-based therapy, and gene therapy.</p></div><div><h3>Results</h3><p>Over the past few years, biomedical engineering has been evolving from the laboratory to clinical applications, for replacement of damaged body tissues due to trauma, cancer, congenital or acquired disorders.</p></div><div><h3>Conclusions</h3><p>This review provides an outlook on the content, benefits, recent advances, limitations, and future expectations of biomedical engineering for salivary glands, oral mucosa, dental structures, and maxillofacial reconstruction.</p></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1013905224001500/pdfft?md5=63ff3f3c3d61fcfec06e241b157427c2&pid=1-s2.0-S1013905224001500-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141028281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Histogical changes in oral mucosa (gingiva) as a method for estimating post-mortem interval: A literature review 以口腔黏膜(牙龈)的组织学变化作为估算死后间隔时间的方法:文献综述
IF 1.8 Q2 Dentistry Pub Date : 2024-05-07 DOI: 10.1016/j.sdentj.2024.05.002
Roben Suhadi Pasaribu, Elza Ibrahim Auerkari, Antonius Winoto Suhartono

Estimating the post-mortem interval (PMI) is an essential step in forensic investigations, particularly those involving homicides and unwitnessed deaths. However, traditional methods occasionally yield inconsistent estimates. Histological and molecular techniques are considered crucial in forensic pathology and are frequently employed to estimate the time interval of death. The gingiva is an oral mucosal tissue used to estimate PMI. This review aimed to examine the potential of histological methods to determine PMI using oral mucosal tissue, namely the gingiva, and to investigate changes that occur in oral mucosal tissue at different time intervals when compared with those in normal tissues. The oral mucosa comprises layers of stratified squamous epithelium and connective tissue. Similar to other body tissues, changes are known to occur in the gingiva after death, and these cellular and tissue changes should also be considered. Alterations in the gingiva include homogenisation, karyorrhexis, pyknosis, karyolysis, chromatin clumping, eosinophilia, collagen fibre degradation, and the loss of tissue architecture. Reviews collating the results of original trials have consistently reported how the oral mucosa is altered by autolysis and how such changes can be observed in histological tissue morphology after death. Histology is an acceptably accurate technique for estimating PMIs.

估算死后间隔时间(PMI)是法医调查的重要步骤,尤其是涉及凶杀案和无证人死亡的调查。然而,传统方法偶尔会产生不一致的估计结果。组织学和分子技术被认为是法医病理学的关键,经常被用来估计死亡时间间隔。牙龈是用于估计 PMI 的口腔黏膜组织。本综述旨在研究利用口腔黏膜组织(即牙龈)确定 PMI 的组织学方法的潜力,并研究口腔黏膜组织与正常组织相比在不同时间间隔内发生的变化。口腔黏膜由分层鳞状上皮和结缔组织组成。与身体其他组织类似,已知牙龈在死亡后也会发生变化,这些细胞和组织变化也应考虑在内。牙龈的变化包括:均质化、核分裂、热解、核溶解、染色质结块、嗜酸性粒细胞增多、胶原纤维降解和组织结构丧失。整理原始试验结果的综述一致报告了自溶如何改变口腔黏膜,以及如何在死后的组织形态学中观察到这些变化。组织学是一种可接受的准确估算 PMI 的技术。
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引用次数: 0
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Saudi Dental Journal
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