Olajide Emmanuel Adedeji, Ju Hyun Min, Gi Eon Park, Hye Jee Kang, Ji-Young Choi, Mariam Omowunmi Aminu, Ocheme Boniface Ocheme, S. Joo, K. Moon, Young Hoon Jung
A biomaterial ink suitable for three-dimensional (3D) printing was developed using cellulose microfibrils (CMFs, 1% w/v) and guar gum (1–7 g/100 mL CMFs), and the post-printing stability and antioxidant functionality of the borax-treated construct were investigated. Rheological analysis, Fourier transform infrared spectrometry, X-ray diffractometry, and scanning electron microscopy revealed the suitability of the two polymers to form an interpenetrating composite hydrogel that would facilitate printability. The produced composite hydrogel showed good structural, morphological, thermal, and textural properties. CMFs with 5% guar gum showing optimal surface properties and rheological properties were printed with the least dimensional errors at 50% infill density, 10 mm/s printing speed, 0.8 mm nozzle diameter, and 0.5 mm layer height. The treatment with borax showed good shape fidelity during 12 h storage. The treated construct also showed considerably increased mechanical properties and antioxidant activities in comparison with the untreated construct. A stable 3D construct suitable for a variety of applications could be produced using CMFs and guar gum-based ink.
采用纤维素微纤维(CMFs, 1% w/v)和瓜尔胶(1-7 g/100 mL CMFs)制备了一种适合三维打印的生物材料墨水，并研究了硼砂处理结构体的打印后稳定性和抗氧化功能。流变分析、傅里叶变换红外光谱、x射线衍射和扫描电子显微镜显示了这两种聚合物形成互穿复合水凝胶的适宜性，这将有利于印刷。制备的复合水凝胶具有良好的结构、形态、热性能和织构性能。在填充密度为50%、打印速度为10 mm/s、喷嘴直径为0.8 mm、层高为0.5 mm的条件下，打印出具有最佳表面性能和流变性能的5%瓜尔胶复合材料，尺寸误差最小。硼砂处理在12 h的贮藏过程中表现出良好的保真度。与未处理的结构相比，处理后的结构也显示出显著增加的机械性能和抗氧化活性。使用CMFs和瓜尔胶基油墨可以产生适合各种应用的稳定3D结构。
{"title":"Development of a 3D-printable matrix using cellulose microfibrils/guar gum-based hydrogels and its post-printing antioxidant activity ","authors":"Olajide Emmanuel Adedeji, Ju Hyun Min, Gi Eon Park, Hye Jee Kang, Ji-Young Choi, Mariam Omowunmi Aminu, Ocheme Boniface Ocheme, S. Joo, K. Moon, Young Hoon Jung","doi":"10.36922/ijb.0164","DOIUrl":"https://doi.org/10.36922/ijb.0164","url":null,"abstract":"A biomaterial ink suitable for three-dimensional (3D) printing was developed using cellulose microfibrils (CMFs, 1% w/v) and guar gum (1–7 g/100 mL CMFs), and the post-printing stability and antioxidant functionality of the borax-treated construct were investigated. Rheological analysis, Fourier transform infrared spectrometry, X-ray diffractometry, and scanning electron microscopy revealed the suitability of the two polymers to form an interpenetrating composite hydrogel that would facilitate printability. The produced composite hydrogel showed good structural, morphological, thermal, and textural properties. CMFs with 5% guar gum showing optimal surface properties and rheological properties were printed with the least dimensional errors at 50% infill density, 10 mm/s printing speed, 0.8 mm nozzle diameter, and 0.5 mm layer height. The treatment with borax showed good shape fidelity during 12 h storage. The treated construct also showed considerably increased mechanical properties and antioxidant activities in comparison with the untreated construct. A stable 3D construct suitable for a variety of applications could be produced using CMFs and guar gum-based ink.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81431369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Congenital abnormalities or acquired trauma to the auricle can result in a need for ear reconstruction and negatively impact a person’s quality of life. Autografting, alloplastic implants, and prostheses are available to treat these issues, but each requires multiple surgical stages and has limitations and complications. Three-dimensional (3D) bioprinting promises to allow the creation of living, patient-specific ear substitutes that could reduce operative morbidity. In this review, we evaluate the current state of 3D bioprinting methods through a systematic search and review of 27 studies, aiming to examine this emerging technology within the context of existing reconstructive options. The included studies were all non-randomized experimental studies, except for a single pilot clinical trial. Most of these studies involved both in vitro and in vivo experiments demonstrating the potential of 3D bioprinting to create functional and anatomically accurate engineered cartilaginous frameworks for surgical implantation. Various ways of optimizing printing were identified, from choosing the most suitable material and cell type for the construct to addressing scaffold deformation and shrinkage issues. 3D printing has the potential to revolutionize reconstructive ear surgery by creating functional and aesthetically pleasing auricles. While more research into printing parameters, bioinks, cell types, and materials could optimize results, the next step is to conduct long-term in vivo clinical trials in humans.
{"title":"3D bioprinting for auricular reconstruction: A review and future perspectives","authors":"Anna Onderková, Deepak M. Kalaskar","doi":"10.36922/ijb.0898","DOIUrl":"https://doi.org/10.36922/ijb.0898","url":null,"abstract":"Congenital abnormalities or acquired trauma to the auricle can result in a need for ear reconstruction and negatively impact a person’s quality of life. Autografting, alloplastic implants, and prostheses are available to treat these issues, but each requires multiple surgical stages and has limitations and complications. Three-dimensional (3D) bioprinting promises to allow the creation of living, patient-specific ear substitutes that could reduce operative morbidity. In this review, we evaluate the current state of 3D bioprinting methods through a systematic search and review of 27 studies, aiming to examine this emerging technology within the context of existing reconstructive options. The included studies were all non-randomized experimental studies, except for a single pilot clinical trial. Most of these studies involved both in vitro and in vivo experiments demonstrating the potential of 3D bioprinting to create functional and anatomically accurate engineered cartilaginous frameworks for surgical implantation. Various ways of optimizing printing were identified, from choosing the most suitable material and cell type for the construct to addressing scaffold deformation and shrinkage issues. 3D printing has the potential to revolutionize reconstructive ear surgery by creating functional and aesthetically pleasing auricles. While more research into printing parameters, bioinks, cell types, and materials could optimize results, the next step is to conduct long-term in vivo clinical trials in humans.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81734475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Perini, V. Palmieri, M. D’Ascenzo, C. Colussi, C. Grassi, G. Friggeri, A. Augello, Li-ying Cui, M. Papi, M. De Spirito
Nerve damage is a prevalent and debilitating condition with limited treatment options. Recent years have seen an increased incidence of neural damage due to factors such as aging populations and traumatic brain injuries. Addressing the urgent need for effective therapies, this study explores the controlled delivery of mesenchymal stem cells (MSCs) secretome, a complex mixture of bioactive factors, which is currently being investigated for its potential in nerve regeneration. The secretome offers significant advantages over stem cells themselves, as it can be more easily characterized and controlled, enabling precise regulation of therapeutic interventions. However, the challenge lies in delivering the secretome specifically to the target anatomical region. To overcome this limitation, we propose a novel approach utilizing near-infrared (NIR) radiation-responsive bioprinted alginate-graphene oxide (AGO) microbeads. Graphene oxide (GO) is a highly biocompatible material with unique properties, including NIR responsiveness, enabling controlled release of therapeutic agents upon NIR exposure. We hypothesized that AGO microbeads could encapsulate MSCs secretome and release it in a controlled manner using NIR radiation. To investigate our hypothesis, controlled damage was induced to hippocampal neurons, and MSCs secretome was encapsulated within AGO microbeads. Subsequently, NIR radiation was applied to trigger the release of the secretome. We compared the efficacy of MSCs secretome with that of astrocytes, which also possess nerve growth and proliferation-promoting capabilities. Our findings demonstrated that the controlled release of MSCs secretome from AGO microbeads through non-invasive NIR radiation significantly promoted the proliferation and regeneration of neurons following nerve injury. AGO microbeads offer multiple advantages over conventional delivery methods, including precise control over the timing, location, and dosage of therapeutic agents. Furthermore, the potential for reduced immunogenicity and tumorigenicity enhances the safety profile of the therapy. Consequently, this study presents a promising avenue for the development of MSC-based therapies for nerve regeneration, with implications for the treatment of various neuropathies and injuries.
{"title":"Near-infrared controlled release of mesenchymal stem cells secretome from bioprinted graphene-based microbeads for nerve regeneration","authors":"G. Perini, V. Palmieri, M. D’Ascenzo, C. Colussi, C. Grassi, G. Friggeri, A. Augello, Li-ying Cui, M. Papi, M. De Spirito","doi":"10.36922/ijb.1045","DOIUrl":"https://doi.org/10.36922/ijb.1045","url":null,"abstract":" Nerve damage is a prevalent and debilitating condition with limited treatment options. Recent years have seen an increased incidence of neural damage due to factors such as aging populations and traumatic brain injuries. Addressing the urgent need for effective therapies, this study explores the controlled delivery of mesenchymal stem cells (MSCs) secretome, a complex mixture of bioactive factors, which is currently being investigated for its potential in nerve regeneration. The secretome offers significant advantages over stem cells themselves, as it can be more easily characterized and controlled, enabling precise regulation of therapeutic interventions. However, the challenge lies in delivering the secretome specifically to the target anatomical region. To overcome this limitation, we propose a novel approach utilizing near-infrared (NIR) radiation-responsive bioprinted alginate-graphene oxide (AGO) microbeads. Graphene oxide (GO) is a highly biocompatible material with unique properties, including NIR responsiveness, enabling controlled release of therapeutic agents upon NIR exposure. We hypothesized that AGO microbeads could encapsulate MSCs secretome and release it in a controlled manner using NIR radiation. To investigate our hypothesis, controlled damage was induced to hippocampal neurons, and MSCs secretome was encapsulated within AGO microbeads. Subsequently, NIR radiation was applied to trigger the release of the secretome. We compared the efficacy of MSCs secretome with that of astrocytes, which also possess nerve growth and proliferation-promoting capabilities. Our findings demonstrated that the controlled release of MSCs secretome from AGO microbeads through non-invasive NIR radiation significantly promoted the proliferation and regeneration of neurons following nerve injury. AGO microbeads offer multiple advantages over conventional delivery methods, including precise control over the timing, location, and dosage of therapeutic agents. Furthermore, the potential for reduced immunogenicity and tumorigenicity enhances the safety profile of the therapy. Consequently, this study presents a promising avenue for the development of MSC-based therapies for nerve regeneration, with implications for the treatment of various neuropathies and injuries.\u0000 ","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84844099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Senmao Wang, Di Wang, Liya Jia, Y. Yue, Genli Wu, Yuyun Chu, Qian Wang, Bo Pan, Haiyue Jiang, Lin Lin
Patient-based training is difficult in ear reconstruction surgery; therefore, costal cartilage models are required for surgical education and pre-operative simulation. Here, we aimed to fabricate personalized models with mechanical and structural similarity to native costal cartilage to simulate ear reconstruction in microtia patients. To achieve this, the stiffness, hardness, and suture retention ability of both native costal cartilage and printed silicone were experimentally examined in vitro. Rheological tests and three-dimensional (3D) comparison methods were used to evaluate the printing ability and outcomes. The printed silicone models were used by residents to practice ear framework handcrafting during ear reconstruction surgery, and the residents’ learning curves were analyzed. In addition, the models were used for pre-operative simulation to study and optimize the surgical plan. The results showed that the consistency of mechanical properties within cartilage and silicone was verified. Printable silicone had good shear-thinning properties, and the printed structures had almost perfect printing fidelity. Residents who used printed silicone models enjoyed great progress and confidence after training. The pre-operative simulation optimized the carving scheme, reduced trauma in the operative site, and avoided wasting necessary cartilage tissue. Overall, fine-fidelity models created in this study were intended for surgical education and pre-operative simulation by applying 3D-printable (3DP) silicone, facilitating the optimization of surgical plans. Surgeons were satisfied with this kind of model and recognized the efficacy and great application value of 3D-printed silicone models for clinical practice.
{"title":"3D printing of costal cartilage models with fine fidelity and biomimetic mechanical performance for ear reconstruction simulation","authors":"Senmao Wang, Di Wang, Liya Jia, Y. Yue, Genli Wu, Yuyun Chu, Qian Wang, Bo Pan, Haiyue Jiang, Lin Lin","doi":"10.36922/ijb.1007","DOIUrl":"https://doi.org/10.36922/ijb.1007","url":null,"abstract":" Patient-based training is difficult in ear reconstruction surgery; therefore, costal cartilage models are required for surgical education and pre-operative simulation. Here, we aimed to fabricate personalized models with mechanical and structural similarity to native costal cartilage to simulate ear reconstruction in microtia patients. To achieve this, the stiffness, hardness, and suture retention ability of both native costal cartilage and printed silicone were experimentally examined in vitro. Rheological tests and three-dimensional (3D) comparison methods were used to evaluate the printing ability and outcomes. The printed silicone models were used by residents to practice ear framework handcrafting during ear reconstruction surgery, and the residents’ learning curves were analyzed. In addition, the models were used for pre-operative simulation to study and optimize the surgical plan. The results showed that the consistency of mechanical properties within cartilage and silicone was verified. Printable silicone had good shear-thinning properties, and the printed structures had almost perfect printing fidelity. Residents who used printed silicone models enjoyed great progress and confidence after training. The pre-operative simulation optimized the carving scheme, reduced trauma in the operative site, and avoided wasting necessary cartilage tissue. Overall, fine-fidelity models created in this study were intended for surgical education and pre-operative simulation by applying 3D-printable (3DP) silicone, facilitating the optimization of surgical plans. Surgeons were satisfied with this kind of model and recognized the efficacy and great application value of 3D-printed silicone models for clinical practice.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2023-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82322553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cardiovascular disease is the world’s leading cause of death, and there is a substantial clinical need for transplantable blood vessels. Through tissue vascular engineering technology, large blood vessel grafts with significant clinical effects have been synthesized. However, synthesizing vascular valves, small vessels up to 6 mm in diameter, and capillary networks up to 500 μm in diameter remains challenging due to the lack of precise manufacturing techniques. In particular, constructing a microvascular network in thick tissue is the technical bottleneck of organ transplantation. Three-dimensional (3D) bioprinting is a computer-assisted layer-by-layer deposition method that can deposit cells and biomaterials at a predetermined location, according to an accurate digital 3D model, to build a delicate and complex bionic structure. This review discusses the progress and limitations of 3D bioprinting in preparing large vessels and valves, small-diameter vessels, and microvascular networks. This paper focuses on improved printing technology and innovative bio-ink materials. The future application of 3D bioprinting is prospected in generating artificial blood vessel grafts and vascularized organs with full biological function.
{"title":"3D bioprinting for vascular grafts and microvasculature","authors":"Junpeng Zhu, Xinwang Wang, Lin Lin, W. Zeng","doi":"10.36922/ijb.0012","DOIUrl":"https://doi.org/10.36922/ijb.0012","url":null,"abstract":"Cardiovascular disease is the world’s leading cause of death, and there is a substantial clinical need for transplantable blood vessels. Through tissue vascular engineering technology, large blood vessel grafts with significant clinical effects have been synthesized. However, synthesizing vascular valves, small vessels up to 6 mm in diameter, and capillary networks up to 500 μm in diameter remains challenging due to the lack of precise manufacturing techniques. In particular, constructing a microvascular network in thick tissue is the technical bottleneck of organ transplantation. Three-dimensional (3D) bioprinting is a computer-assisted layer-by-layer deposition method that can deposit cells and biomaterials at a predetermined location, according to an accurate digital 3D model, to build a delicate and complex bionic structure. This review discusses the progress and limitations of 3D bioprinting in preparing large vessels and valves, small-diameter vessels, and microvascular networks. This paper focuses on improved printing technology and innovative bio-ink materials. The future application of 3D bioprinting is prospected in generating artificial blood vessel grafts and vascularized organs with full biological function. ","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77661715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liang Chen, Guowei Huang, Ming-Han Yu, Yang Liu, Tao Cheng, Aiguo Li, Wen Wang, Shengnan Qin
The structure and composition of articular cartilage is complex, and its self-healing ability is limited, and thus, it is difficult to achieve ideal healing once the articular cartilage is damaged. Recently, three-dimensional (3D) printing technology has provided a new possibility for the repair of articular cartilage. Engineered cartilage tissues can be fabricated by superimposing customized inks, considering different geometric structures and components of tissues. 3D printing can be effectively used to manufacture high-precision structures with complex geometry, solving the shortcomings of traditional scaffold fabrication techniques. Gelatin methacrylate (GelMA) is modified gelatin and is currently a widely used 3D printing ink due to its photocrosslinking properties. With good biocompatibility and tunable physical properties, it can provide a good scaffold platform for cell proliferation and growth factor release. Given that the role of 3D printing technology in cartilage repair has been widely reported, this article reviews the research progress of 3D-printed GelMA-based biomaterials in articular cartilage tissue engineering. We focus primarily on how 3D printing technology addresses the existing challenges inherent to the field of articular cartilage tissue engineering. We accentuate the modifications implemented in GelMA-based 3D printing scaffolds to optimize articular cartilage regeneration. Additionally, we provide a comprehensive summary of the utilization of GelMA-based biomaterials incorporating various cells, growth factors, or other tissue components and highlight how these adaptations, in conjunction with the benefits of 3D printing technology, facilitate improvements the articular cartilage repair.
{"title":"Recent progress on 3D-printed gelatin methacrylate-based biomaterials for articular cartilage repair ","authors":"Liang Chen, Guowei Huang, Ming-Han Yu, Yang Liu, Tao Cheng, Aiguo Li, Wen Wang, Shengnan Qin","doi":"10.36922/ijb.0116","DOIUrl":"https://doi.org/10.36922/ijb.0116","url":null,"abstract":"The structure and composition of articular cartilage is complex, and its self-healing ability is limited, and thus, it is difficult to achieve ideal healing once the articular cartilage is damaged. Recently, three-dimensional (3D) printing technology has provided a new possibility for the repair of articular cartilage. Engineered cartilage tissues can be fabricated by superimposing customized inks, considering different geometric structures and components of tissues. 3D printing can be effectively used to manufacture high-precision structures with complex geometry, solving the shortcomings of traditional scaffold fabrication techniques. Gelatin methacrylate (GelMA) is modified gelatin and is currently a widely used 3D printing ink due to its photocrosslinking properties. With good biocompatibility and tunable physical properties, it can provide a good scaffold platform for cell proliferation and growth factor release. Given that the role of 3D printing technology in cartilage repair has been widely reported, this article reviews the research progress of 3D-printed GelMA-based biomaterials in articular cartilage tissue engineering. We focus primarily on how 3D printing technology addresses the existing challenges inherent to the field of articular cartilage tissue engineering. We accentuate the modifications implemented in GelMA-based 3D printing scaffolds to optimize articular cartilage regeneration. Additionally, we provide a comprehensive summary of the utilization of GelMA-based biomaterials incorporating various cells, growth factors, or other tissue components and highlight how these adaptations, in conjunction with the benefits of 3D printing technology, facilitate improvements the articular cartilage repair.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80578932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fan Xu, Shunli Rui, Cheng Yang, Xiaoyan Jiang, Wei Wu, Xianlun Tang, David G Armstrong, Yu Ma, Wu Deng
Most conventional therapies have limitations in the repair of complex wounds caused by chronic inflammation in patients with diabetic foot ulcers (DFUs). In response to the demand for more biotechnology strategies, bioprinting has been explored in the regeneration field in recent years. However, challenges remain regarding the structure of complex models and the selection of proper biomaterials. The purpose of this review is to introduce the current applications of bioprinting technology in chronic diabetic foot wound healing. First, the most common application of bioprinting in producing skin equivalents to promote wound healing is introduced; second, functional improvements in the treatment of chronic and difficult-to-heal DFU wounds facilitated by bioprinting applications are discussed; and last but not least, bioprinting applications in addressing unique diabetic foot disease characteristics are summarized. Furthermore, the present work summarizes material selection and correlations between three-dimensional (3D) bioprinting and a variety of biomimetic strategies for accelerating wound healing. Novel, biotechnological tools such as organoids for developing new biomaterials for bioprinting in the future are also discussed.
{"title":"Bioprinting technology for the management of diabetic foot disease: Emerging applications, challenges, and prospects","authors":"Fan Xu, Shunli Rui, Cheng Yang, Xiaoyan Jiang, Wei Wu, Xianlun Tang, David G Armstrong, Yu Ma, Wu Deng","doi":"10.36922/ijb.0142","DOIUrl":"https://doi.org/10.36922/ijb.0142","url":null,"abstract":"Most conventional therapies have limitations in the repair of complex wounds caused by chronic inflammation in patients with diabetic foot ulcers (DFUs). In response to the demand for more biotechnology strategies, bioprinting has been explored in the regeneration field in recent years. However, challenges remain regarding the structure of complex models and the selection of proper biomaterials. The purpose of this review is to introduce the current applications of bioprinting technology in chronic diabetic foot wound healing. First, the most common application of bioprinting in producing skin equivalents to promote wound healing is introduced; second, functional improvements in the treatment of chronic and difficult-to-heal DFU wounds facilitated by bioprinting applications are discussed; and last but not least, bioprinting applications in addressing unique diabetic foot disease characteristics are summarized. Furthermore, the present work summarizes material selection and correlations between three-dimensional (3D) bioprinting and a variety of biomimetic strategies for accelerating wound healing. Novel, biotechnological tools such as organoids for developing new biomaterials for bioprinting in the future are also discussed.\u0000 ","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82175129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ruiquan Liu, Litao Jia, Jianguo Chen, Y. Long, Jinshi Zeng, Siyu Liu, Bo Pan, Xia Liu, Haiyue Jiang
Three-dimensional (3D) bioprinting has brought new promising strategies for the regeneration of cartilage with specific shapes. In cartilage bioprinting, chondrocyte-laden hydrogels are the most commonly used bioinks. However, the dispersion of cells and the dense texture of the hydrogel in the conventional bioink may limit cell–cell/ cell–extracellular matrix (ECM) interactions, counting against cartilage regeneration and maturation. To address this issue, in this study, we developed a functional bioink for cartilage bioprinting based on chondrocyte spheroids (CSs) and microporous hydrogels, in which CSs as multicellular aggregates can provide extensive cell– cell/cell–ECM interactions to mimic the natural cartilage microenvironment, and microporous hydrogels can provide space and channel for the growth and fusion of the CSs. Firstly, we used a non-adhesive microporous system to produce homogeneous self-assembled CSs in high-throughput and evaluated the influence of different CSs preparation parameters (cell number and culture time) on CSs, which aids in the preparation of bioink suitable for cartilage bioprinting. Then, polyethylene oxide (PEO) was introduced into gelatin methacrylate (GelMA) to prepare microporous hydrogel. Finally, the CS-laden microporous hydrogels were printed, and the constructs were implanted into nude mice. The results showed that the CSs with 500 cells cultured for 1 day exhibited better proliferation and growth ability in microporous hydrogels compared to those with more cells and cultured for longer time. In addition, the results also demonstrated that the CS-laden bioink can be successfully printed into predefined lattice-shape constructs with little cell damage and regenerated cartilage tissue in vivo with a structure similar to natural cartilage characterized by typical lacunae structure and abundant cartilage-specific ECM deposition. In summary, our study verified the feasibility and advantages of using CSs as building blocks in cartilage bioprinting, which provides novel strategies for the fabrication and regeneration of patient-specific shaped cartilage.
{"title":"Chondrocyte spheroid-laden microporous hydrogel-based 3D bioprinting for cartilage regeneration","authors":"Ruiquan Liu, Litao Jia, Jianguo Chen, Y. Long, Jinshi Zeng, Siyu Liu, Bo Pan, Xia Liu, Haiyue Jiang","doi":"10.36922/ijb.0161","DOIUrl":"https://doi.org/10.36922/ijb.0161","url":null,"abstract":"Three-dimensional (3D) bioprinting has brought new promising strategies for the regeneration of cartilage with specific shapes. In cartilage bioprinting, chondrocyte-laden hydrogels are the most commonly used bioinks. However, the dispersion of cells and the dense texture of the hydrogel in the conventional bioink may limit cell–cell/ cell–extracellular matrix (ECM) interactions, counting against cartilage regeneration and maturation. To address this issue, in this study, we developed a functional bioink for cartilage bioprinting based on chondrocyte spheroids (CSs) and microporous hydrogels, in which CSs as multicellular aggregates can provide extensive cell– cell/cell–ECM interactions to mimic the natural cartilage microenvironment, and microporous hydrogels can provide space and channel for the growth and fusion of the CSs. Firstly, we used a non-adhesive microporous system to produce homogeneous self-assembled CSs in high-throughput and evaluated the influence of different CSs preparation parameters (cell number and culture time) on CSs, which aids in the preparation of bioink suitable for cartilage bioprinting. Then, polyethylene oxide (PEO) was introduced into gelatin methacrylate (GelMA) to prepare microporous hydrogel. Finally, the CS-laden microporous hydrogels were printed, and the constructs were implanted into nude mice. The results showed that the CSs with 500 cells cultured for 1 day exhibited better proliferation and growth ability in microporous hydrogels compared to those with more cells and cultured for longer time. In addition, the results also demonstrated that the CS-laden bioink can be successfully printed into predefined lattice-shape constructs with little cell damage and regenerated cartilage tissue in vivo with a structure similar to natural cartilage characterized by typical lacunae structure and abundant cartilage-specific ECM deposition. In summary, our study verified the feasibility and advantages of using CSs as building blocks in cartilage bioprinting, which provides novel strategies for the fabrication and regeneration of patient-specific shaped cartilage.\u0000 ","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78358794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Ianchiș, Maria Minodora Marin, Rebeca Leu Alexa, Ioana Catalina Gifu, E. Alexandrescu, G. Pîrcălăbioru, George Mihail Vlasceanu, George Mihail Teodorescu, A. Serafim, S. Preda, Cristina Lavinia Nistor, C. Petcu
The main objective of the present work was to produce three-dimensional (3D)- printable nanocomposite hydrogels based on two kinds of marine-sourced polysaccharides doped with nanoclay with potential biomedical application. First part of the research study investigated the preparation of the polysaccharide bicomponent hydrogel formulations followed by the selection of the optimal ratio of polysaccharides concentrations which ensured proper morphostructural stability of the 3D-printed constructs. Second step aimed to generate 3D scaffolds with high printing fidelity by modulating the nanoclay amount doped within the previously selected biopolymer ink. In compliance with the additive manufacturing experiments, the alginate–salecan hydrogels enriched with the highest nanofiller concentrations demonstrated the highest suitability for 3D printing process. The morphological and structural studies confirmed the ability of the nanocomposite formulations to efficiently produce porous 3D-printed constructs with improved fidelity. The morphostructural findings underlined the implication of choosing the appropriate ratio between components, as they have a considerable impact on the functionality of printing formulations and subsequent 3D-printed structures. Hence, from the obtained results, these novel hydrogel nanocomposites inks are considered valuable biomaterials with suitable features for applications in the additive manufacturing of 3D structures with precise shape for customized regenerative therapy.
{"title":"Nanoclay-reinforced alginate/salecan composite inks for 3D printing applications","authors":"R. Ianchiș, Maria Minodora Marin, Rebeca Leu Alexa, Ioana Catalina Gifu, E. Alexandrescu, G. Pîrcălăbioru, George Mihail Vlasceanu, George Mihail Teodorescu, A. Serafim, S. Preda, Cristina Lavinia Nistor, C. Petcu","doi":"10.36922/ijb.0967","DOIUrl":"https://doi.org/10.36922/ijb.0967","url":null,"abstract":"The main objective of the present work was to produce three-dimensional (3D)- printable nanocomposite hydrogels based on two kinds of marine-sourced polysaccharides doped with nanoclay with potential biomedical application. First part of the research study investigated the preparation of the polysaccharide bicomponent hydrogel formulations followed by the selection of the optimal ratio of polysaccharides concentrations which ensured proper morphostructural stability of the 3D-printed constructs. Second step aimed to generate 3D scaffolds with high printing fidelity by modulating the nanoclay amount doped within the previously selected biopolymer ink. In compliance with the additive manufacturing experiments, the alginate–salecan hydrogels enriched with the highest nanofiller concentrations demonstrated the highest suitability for 3D printing process. The morphological and structural studies confirmed the ability of the nanocomposite formulations to efficiently produce porous 3D-printed constructs with improved fidelity. The morphostructural findings underlined the implication of choosing the appropriate ratio between components, as they have a considerable impact on the functionality of printing formulations and subsequent 3D-printed structures. Hence, from the obtained results, these novel hydrogel nanocomposites inks are considered valuable biomaterials with suitable features for applications in the additive manufacturing of 3D structures with precise shape for customized regenerative therapy.\u0000 ","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79926442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haoyu Li, Huixing Zhou, Chongwen Xu, Yen Wei, Xiuying Tang
The biofabrication of multi-cellular tissues or organoids (MTOs) has been challenging in regenerative medicine for decades. Currently, two primary technological approaches are being explored: scaffold-based strategies utilizing three-dimensional (3D) bioprinting techniques and scaffold-free strategies employing bioassembly techniques. 3D bioprinting techniques include jetting-based, extrusion-based, and vat photopolymerization-based methods, and bioassembly techniques include Kenzan, fluid-based manipulation and microfluid, bioprinting-assisted tissue emergence, and aspiration-assisted technology methods. Scaffold-based strategies primarily concentrate on the construction of scaffold structures to provide an extracellular environment, while scaffold-free strategies primarily emphasize the assembly methods of building blocks. Different biofabrication technologies have their advantages and limitations. This review provides an overview of the mechanisms, advantages, and limitations of scaffold-based and scaffold-free strategies in tissue engineering. It also compares the strengths and weaknesses of these two strategies, along with their respective suitability under different conditions. Moreover, the significant challenges in the future development of convergence strategies, specifically the integration of scaffold-based and scaffold-free approaches, are examined in an objective manner. This review concludes that integrating scaffold-based and scaffold-free strategies could overcome the problems in the biofabrication of MTOs. A novel fabrication method, the BioMicroMesh method, is proposed based on the convergence strategy. Concurrently, the development of a desktop-scale integrated intelligent biofabrication device, the BioMicroMesh system, is underway. This system is tailored to the BioMicroMesh method and incorporates cell aggregate spheroids preparation, 3D bioprinting, bioassembly, and multi-organoid co-culture functions, providing an objective perspective on its capabilities.
{"title":"3D bioprinting and scaffold-free strategies for fabrication of multi-cellular tissues or organoids","authors":"Haoyu Li, Huixing Zhou, Chongwen Xu, Yen Wei, Xiuying Tang","doi":"10.36922/ijb.0135","DOIUrl":"https://doi.org/10.36922/ijb.0135","url":null,"abstract":"The biofabrication of multi-cellular tissues or organoids (MTOs) has been challenging in regenerative medicine for decades. Currently, two primary technological approaches are being explored: scaffold-based strategies utilizing three-dimensional (3D) bioprinting techniques and scaffold-free strategies employing bioassembly techniques. 3D bioprinting techniques include jetting-based, extrusion-based, and vat photopolymerization-based methods, and bioassembly techniques include Kenzan, fluid-based manipulation and microfluid, bioprinting-assisted tissue emergence, and aspiration-assisted technology methods. Scaffold-based strategies primarily concentrate on the construction of scaffold structures to provide an extracellular environment, while scaffold-free strategies primarily emphasize the assembly methods of building blocks. Different biofabrication technologies have their advantages and limitations. This review provides an overview of the mechanisms, advantages, and limitations of scaffold-based and scaffold-free strategies in tissue engineering. It also compares the strengths and weaknesses of these two strategies, along with their respective suitability under different conditions. Moreover, the significant challenges in the future development of convergence strategies, specifically the integration of scaffold-based and scaffold-free approaches, are examined in an objective manner. This review concludes that integrating scaffold-based and scaffold-free strategies could overcome the problems in the biofabrication of MTOs. A novel fabrication method, the BioMicroMesh method, is proposed based on the convergence strategy. Concurrently, the development of a desktop-scale integrated intelligent biofabrication device, the BioMicroMesh system, is underway. This system is tailored to the BioMicroMesh method and incorporates cell aggregate spheroids preparation, 3D bioprinting, bioassembly, and multi-organoid co-culture functions, providing an objective perspective on its capabilities.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77178097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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