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Design and fused deposition modeling of triply periodic minimal surface scaffolds with channels and hydrogel for breast reconstruction. 乳房再造用三周期最小表面通道和水凝胶支架的设计和熔融沉积建模。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.685
Xiaolong Zhu, Feng Chen, Hong Cao, Ling Li, Ning He, Xiaoxiao Han

3D-printed scaffolds that forge a new path for regenerative medicine are widely used in breast reconstruction due to their personalized shape and adjustable mechanical properties. However, the elastic modulus of present breast scaffolds is significantly higher than that of native breast tissue, leading to insufficient stimulation for cell differentiation and tissue formation. In addition, the lack of a tissue-like environment results in breast scaffolds being difficult to promote cell growth. This paper presents a geometrically new scaffold, featuring a triply periodic minimal surface (TPMS) that ensures structural stability and multiple parallel channels that can modulate elastic modulus as required. The geometrical parameters for TPMS and parallel channels were optimized to obtain ideal elastic modulus and permeability through numerical simulations. The topologically optimized scaffold integrated with two types of structures was then fabricated using fused deposition modeling. Finally, the poly (ethylene glycol) diacrylate/gelatin methacrylate hydrogel loaded with human adipose-derived stem cells was incorporated into the scaffold by perfusion and ultraviolet curing for improvement of the cell growth environment. Compressive experiments were also performed to verify the mechanical performance of the scaffold, demonstrating high structural stability, appropriate tissue-like elastic modulus (0.2 - 0.83 MPa), and rebound capability (80% of the original height). In addition, the scaffold exhibited a wide energy absorption window, offering reliable load buffering capability. The biocompatibility was also confirmed by cell live/dead staining assay.

3d打印支架由于其个性化的形状和可调节的力学性能,在乳房重建中得到了广泛的应用,为再生医学开辟了一条新的道路。然而,目前乳腺支架的弹性模量明显高于天然乳腺组织,导致对细胞分化和组织形成的刺激不足。此外,缺乏组织样环境导致乳腺支架难以促进细胞生长。本文提出了一种几何上新的支架,具有三周期最小表面(TPMS),确保结构稳定性和多个平行通道,可以根据需要调节弹性模量。通过数值模拟,优化了TPMS和平行通道的几何参数,获得了理想的弹性模量和渗透率。然后采用熔融沉积建模的方法制备了两种结构集成的拓扑优化支架。最后,将载人脂肪干细胞的聚(乙二醇)二丙烯酸酯/明胶甲基丙烯酸酯水凝胶通过灌注和紫外线固化加入支架中,改善细胞生长环境。压缩实验验证了支架的力学性能,表明支架具有较高的结构稳定性、适宜的类组织弹性模量(0.2 - 0.83 MPa)和回弹能力(原高度的80%)。此外,该支架具有较宽的能量吸收窗口,提供可靠的负载缓冲能力。细胞活/死染色法证实了其生物相容性。
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引用次数: 2
State-of-the-art techniques for promoting tissue regeneration: Combination of three-dimensional bioprinting and carbon nanomaterials. 促进组织再生的最新技术:三维生物打印和碳纳米材料的结合。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.v9i1.635
Iruthayapandi Selestin Raja, Moon Sung Kang, Suck Won Hong, Hojae Bae, Bongju Kim, Yu-Shik Hwang, Jae Min Cha, Dong-Wook Han

181Biofabrication approaches, such as three-dimensional (3D) bioprinting of hydrogels, have recently garnered increasing attention, especially in the construction of 3D structures that mimic the complexity of tissues and organs with the capacity for cytocompatibility and post-printing cellular development. However, some printed gels show poor stability and maintain less shape fidelity if parameters such as polymer nature, viscosity, shear-thinning behavior, and crosslinking are affected. Therefore, researchers have incorporated various nanomaterials as bioactive fillers into polymeric hydrogels to address these limitations. Carbon-family nanomaterials (CFNs), hydroxyapatites, nanosilicates, and strontium carbonates have been incorporated into printed gels for application in various biomedical fields. In this review, following the compilation of research publications on CFNs-containing printable gels in various tissue engineering applications, we discuss the types of bioprinters, the prerequisites of bioink and biomaterial ink, as well as the progress and challenges of CFNs-containing printable gels in this field.

生物制造方法,如水凝胶的三维(3D)生物打印,最近引起了越来越多的关注,特别是在模拟组织和器官复杂性的3D结构的构建方面,具有细胞相容性和打印后细胞发育的能力。然而,如果聚合物性质、粘度、剪切减薄行为和交联等参数受到影响,一些打印凝胶的稳定性较差,形状保真度较低。因此,研究人员将各种纳米材料作为生物活性填料加入到聚合物水凝胶中,以解决这些限制。碳族纳米材料(CFNs)、羟基磷灰石、纳米硅酸盐和碳酸锶已经被加入到打印凝胶中,用于各种生物医学领域。本文综述了含cfns可打印凝胶在各种组织工程中的研究成果,讨论了生物打印机的类型、生物墨水和生物材料墨水的前提条件,以及含cfns可打印凝胶在该领域的进展和挑战。
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引用次数: 3
Combination of 3D printing and electrospinning to develop chitin/gelatin/PVA scaffolds. 结合3D打印和静电纺丝技术开发甲壳素/明胶/聚乙烯醇支架。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.701
Teresa Carranza, Jone Uranga, Ainhoa Irastorza, Ander Izeta, Pedro Guerrero, Koro de la Caba

In this study, novel scaffolds based on natural polymers were developed by combining 3D printing (3DP) and electrospinning (ES) techniques. ES ink was prepared with gelatin and poly(vinyl alcohol) (PVA), while 3DP ink was prepared with gelatin and chitin. Different biopolymers were used to confer unique properties to each ink and obtain a multilayered scaffold suitable for tissue regeneration. First, gelatin is able to exhibit the characteristics needed for both inks since gelatin chains contain arginineglycine-aspartic (RGD) motifs, an important sequence in the promotion of cell adhesion, which gives gelatin an improved biological behavior in comparison to other polymers. Additionally, PVA was selected for ES ink to facilitate gelatin spinnability, and chitin was incorporated into 3DP ink as reinforcement to provide mechanical support and protection to the overall design. In this work, chitin was extracted from fruit fly pupae. The high extraction yield and purity of the chitin obtained from the fruit fly pupae confirmed that this pupa is an alternative source to produce chitin. Once the chitin was characterized, both inks were prepared and rheological analysis was carried out in order to confirm the shear thinning behavior required for additive manufacturing processes. The combination of 3DP and ES processes resulted in porous scaffolds, which were proven biocompatible, highlighting their potential for biomedical applications.

本研究将3D打印(3DP)和静电纺丝(ES)技术相结合,开发了基于天然聚合物的新型支架。用明胶和聚乙烯醇(PVA)制备ES油墨,用明胶和甲壳素制备3DP油墨。不同的生物聚合物被用来赋予每种墨水独特的性能,并获得适合组织再生的多层支架。首先,明胶能够表现出两种油墨所需的特性,因为明胶链含有精氨酸甘氨酸天冬氨酸(RGD)基序,这是促进细胞粘附的重要序列,与其他聚合物相比,这使明胶具有更好的生物行为。此外,ES油墨选择了PVA,以促进明胶的可纺性,并在3d打印油墨中加入几丁质作为增强剂,为整体设计提供机械支撑和保护。本研究从果蝇蛹中提取甲壳素。从果蝇蛹中提取的几丁质提取率高,纯度高,证实了该蛹是生产几丁质的替代来源。一旦甲壳素被表征,两种油墨都被制备,并进行流变分析,以确认增材制造工艺所需的剪切减薄行为。3d打印和ES工艺的结合产生了多孔支架,这些支架被证明具有生物相容性,突出了它们在生物医学应用方面的潜力。
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引用次数: 0
The design and processing of a 3D-printed high-performance biological fixation plate. 3d打印高性能生物固定板的设计与加工。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.v9i2.658
Zhang Guoqing, Li Junxin, Zhou Xiaoyu, Zhou Yongsheng, Yuchao Bai

In order to generate a high-performance personalized biological fixation plate with matching mechanical properties and biocompatibility, reverse reconstruction and fracture reduction of a femur were performed by combining reverse and forward approaches, and the surface was extracted according to the installation position of the plate to complete plate modeling by shifting, thickening, and performing other operations. Subsequently, topology optimization and three-dimensional (3D) printing were performed, and the properties of the manufactured plate were probed. The results showed that the maximum displacement of the plate was 4.13 mm near the femoral head, the maximum stress was 5.15e2 MPa on both sides of the plate across its entire length, and the stress concentration decreased following topology optimization. The plate with optimized topology and filled with porous structure has a good filling effect. The final mass of the H-shaped plate was 12.05 g, while that of the B-shaped plate was 11.05 g, which dropped by 20.93% and 27.49%, respectively, compared with the original plate. The surface of the 3D-printed plate was bright and new, with a clear pore structure and good lap joint. The B-shaped and H-shaped plates were closely dovetailed with the host bone, which met the assembly requirements. This lays a foundation for the direct application of a high-performance personalized biological fixation plate.

为了制作出力学性能和生物相容性匹配的高性能个性化生物固定钢板,采用正向和反向相结合的方法对股骨进行反向重建和骨折复位,并根据钢板的安装位置提取表面,通过移位、加厚等操作完成钢板建模。随后,进行了拓扑优化和三维(3D)打印,并对所制板的性能进行了探测。结果表明,钢板在股骨头附近的最大位移为4.13 mm,整个长度上钢板两侧的最大应力为5.15e2 MPa,拓扑优化后应力集中减小。经优化的拓扑结构和多孔结构填充板具有良好的填充效果。h形板的最终质量为12.05 g, b形板的最终质量为11.05 g,分别比原板下降了20.93%和27.49%。3d打印板表面光洁新颖,孔隙结构清晰,搭接良好。b型板和h型板与宿主骨紧密吻合,满足装配要求。这为高性能个性化生物固定板的直接应用奠定了基础。
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引用次数: 0
Personalized 3D-printed amniotic fornical ring for ocular surface reconstruction. 用于眼表重建的个性化3d打印羊膜正式环。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.713
Nan Zhang, Ruixing Liu, Xiaowu Liu, Songlin Hou, Runan Dou, Xingchen Geng, Yan Li, Jingguo Li, Lei Zhu, Zhanrong Li

In the present work, we used three-dimensional (3D) printing technology to make a polylactic acid (PLA) amniotic fornical ring (AFR) for ocular surface reconstruction. This work is a retrospective and interventional case series of patients with ocular surface diseases who underwent either personalized 3D-printed AFR-assisted amniotic membrane transplantation (AMT) or sutured AMT (SAMT). Patient epidemiology, treatment, operative duration, epithelial healing time, retention time, vision changes, morbidity, and costs were analyzed. Thirty-one patients (40 eyes) and 19 patients (22 eyes) were enrolled in the 3D-printed AFR group and the SAMT group, respectively. The clinical indications of AFR and SAMT were similar, such as corneal and/or conjunctival epithelial defects due to chemical burns, thermal burns, Stevens-Johnson syndrome (SJS), or toxic epidermal necrolysis (TEN). The mean dissolution time was 15 ± 11 days in the AFR group, compared with 14 ± 7 days in the SAMT group. The percentage of healed corneal area was 90.91% (66.10%-100.00%) for AFR and 93.67% (60.23%-100.00%) for SAMT. The median time for corneal epithelial healing was 14 (7-75) days in the AFR group and 30 (14-55) days in the suture AMT group. There were no significant differences in the initial visual acuity, final visual acuity, or improvement in visual acuity between the two groups. The operation duration in the AFR group was significantly shorter than that in the SAMT group. Regarding the cost analysis, the average cost per eye in the AFR group was significantly lower than that in the SAMT group. Furthermore, 3D-printed and sterile AFR showed no obvious side effects on the eyes. Our results suggested that 3D-printed PLA scaffolds could be used as an AFR device for ocular surface disease. In addition, personalized 3D-printed AFR is superior to conventional AMT in operation duration and cost effectiveness, thereby reducing the financial burden on our health care system.

在本工作中,我们采用三维(3D)打印技术制作了用于眼表重建的聚乳酸(PLA)羊膜形环(AFR)。本研究是对接受个性化3d打印afr辅助羊膜移植(AMT)或缝合羊膜移植(SAMT)的眼表疾病患者的回顾性和干预性病例系列。分析患者流行病学、治疗、手术时间、上皮愈合时间、保留时间、视力变化、发病率和费用。3d打印AFR组31例(40只眼),SAMT组19例(22只眼)。AFR和SAMT的临床适应症相似,如化学烧伤、热烧伤、Stevens-Johnson综合征(SJS)或中毒性表皮坏死松解(TEN)所致的角膜和/或结膜上皮缺损。AFR组平均溶出时间为15±11天,SAMT组平均溶出时间为14±7天。AFR组角膜愈合面积占90.91% (66.10% ~ 100.00%),SAMT组角膜愈合面积占93.67%(60.23% ~ 100.00%)。AFR组角膜上皮愈合的中位时间为14(7-75)天,缝合AMT组为30(14-55)天。两组患者的初始视力、最终视力及视力改善均无显著差异。AFR组的手术时间明显短于SAMT组。在成本分析方面,AFR组每只眼的平均成本显著低于SAMT组。此外,3d打印无菌AFR对眼睛没有明显的副作用。我们的研究结果表明,3d打印PLA支架可以作为眼表疾病的AFR装置。此外,个性化3d打印AFR在操作时间和成本效益方面优于传统AMT,从而减轻了医疗保健系统的经济负担。
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引用次数: 1
3D-printed gradient scaffolds for osteochondral defects: Current status and perspectives. 3d打印梯度骨软骨缺损支架:现状与展望。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.724
Jianhang Du, Ziqing Zhu, Jia Liu, Xiaogang Bao, Qian Wang, Changgui Shi, Chaoqian Zhao, Guohua Xu, Dejian Li

Articular osteochondral defects are quite common in clinical practice, and tissue engineering techniques can offer a promising therapeutic option to address this issue.The articular osteochondral unit comprises hyaline cartilage, calcified cartilage zone (CCZ), and subchondral bone.As the interface layer of articular cartilage and bone, the CCZ plays an essentialpart in stress transmission and microenvironmental regulation.Osteochondral scaffolds with the interface structure for defect repair are the future direction of tissue engineering. Three-dimensional (3D) printing has the advantages of speed, precision, and personalized customization, which can satisfy the requirements of irregular geometry, differentiated composition, and multilayered structure of articular osteochondral scaffolds with boundary layer structure. This paper summarizes the anatomy, physiology, pathology, and restoration mechanisms of the articular osteochondral unit, and reviews the necessity for a boundary layer structure in osteochondral tissue engineering scaffolds and the strategy for constructing the scaffolds using 3D printing. In the future, we should not only strengthen the basic research on osteochondral structural units, but also actively explore the application of 3D printing technology in osteochondral tissue engineering. This will enable better functional and structural bionics of the scaffold, which ultimately improve the repair of osteochondral defects caused by various diseases.

关节骨软骨缺损在临床实践中非常常见,组织工程技术可以为解决这一问题提供有前途的治疗选择。关节骨软骨单元包括透明软骨、钙化软骨带(CCZ)和软骨下骨。CCZ作为关节软骨和骨的界面层,在应力传递和微环境调节中起着重要作用。具有界面结构的骨软骨支架是未来组织工程的发展方向。三维打印具有速度快、精度高、个性化定制等优点,可以满足具有边界层结构的关节骨软骨支架几何形状不规则、成分差异化、结构多层次的要求。本文综述了关节骨软骨单元的解剖、生理、病理和修复机制,综述了骨软骨组织工程支架中边界层结构的必要性以及3D打印构建骨软骨支架的策略。未来,我们不仅要加强对骨软骨结构单元的基础研究,还要积极探索3D打印技术在骨软骨组织工程中的应用。这将使支架具有更好的功能和结构仿生学,最终改善各种疾病引起的骨软骨缺损的修复。
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引用次数: 0
Research progress and challenges of bioprinting in wound dressing and healing: Bibliometrics-based analysis and perspectives. 生物打印在伤口敷料和愈合中的研究进展和挑战:基于文献计量学的分析和观点。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.v9i2.653
Shuduan Mao, Junjie Man, Jialei Wang, Li Fu, Chengliang Yin, Hassan Karimi-Maleh

As the body's largest organ, the skin has important roles in barrier function, immune response, prevention of water loss and excretion of waste. Patients with extensive and severe skin lesions would die due to insufficient graftable skin. Commonly used treatments include autologous skin grafts, allogeneic/allogeneic skin grafts, cytoactive factors, cell therapy, and dermal substitutes. However, traditional treatment methods are still inadequate regarding skin repair time, treatment costs, and treatment results. In recent years, the rapid development of bioprinting technology has provided new ideas to solve the above-mentioned challenges. This review describes the principles of bioprinting technology and research advances in wound dressing and healing. This review features a data mining and statistical analysis of this topic through bibliometrics. The annual publications on this topic, participating countries, and institutions were used to understand the development history. Keyword analysis was used to understand the focus of investigation and challenges in this topic. According to bibliometric analysis, bioprinting in wound dressing and healing is in an explosive phase, and future research should focus on discovering new cell sources, innovative bioink development, and developing large-scale printing technology processes.

作为人体最大的器官,皮肤在屏障功能、免疫反应、防止水分流失和排泄废物方面发挥着重要作用。大面积严重皮肤病变的患者会因皮肤移植不足而死亡。常用的治疗方法包括自体皮肤移植、同种异体/异体皮肤移植、细胞活性因子、细胞疗法和真皮替代品。然而,传统的治疗方法在皮肤修复时间、治疗费用和治疗效果等方面仍存在不足。近年来,生物打印技术的快速发展为解决上述挑战提供了新的思路。本文综述了生物打印技术的原理及其在伤口敷料和愈合方面的研究进展。这篇综述的特点是通过文献计量学对这个主题进行数据挖掘和统计分析。利用关于这一主题的年度出版物、参与国和机构来了解发展历史。通过关键词分析来了解本课题的研究重点和面临的挑战。文献计量学分析认为,生物打印在伤口敷料和愈合中的应用正处于爆发式发展阶段,未来的研究重点应放在发现新的细胞来源、创新生物墨水开发、开发大规模打印技术工艺等方面。
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引用次数: 1
Experimental study on repair of cartilage defects in the rabbits with GelMA-MSCs scaffold prepared by three-dimensional bioprinting. 三维生物打印制备GelMA-MSCs支架修复兔软骨缺损的实验研究。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.v9i2.662
Zijie Pei, Mingyang Gao, Junhui Xing, Changbao Wang, Piqian Zhao, Hongtao Zhang, Jing Qu

Cartilage damage is a common orthopedic disease, which can be caused by sports injury, obesity, joint wear, and aging, and cannot be repaired by itself. Surgical autologous osteochondral grafting is often required in deep osteochondral lesions to avoid the later progression of osteoarthritis. In this study, we fabricated a gelatin methacryloyl-marrow mesenchymal stem cells (GelMA-MSCs) scaffold by three-dimensional (3D) bioprinting. This bioink is capable of fast gel photocuring and spontaneous covalent cross-linking, which can maintain high viability of MSCs and provide a benign microenvironment to promote the interaction, migration, and proliferation of cells. In vivo experiments, further, proved that the 3D bioprinting scaffold can promote the regeneration of cartilage collagen fibers and have a remarkable effect on cartilage repair of rabbit cartilage injury model, which may represent a general and versatile strategy for precise engineering of cartilage regeneration system.

软骨损伤是一种常见的骨科疾病,可由运动损伤、肥胖、关节磨损、衰老等原因引起,不能自行修复。手术自体骨软骨移植通常需要在深度骨软骨病变,以避免骨关节炎的后期进展。在这项研究中,我们通过三维生物打印技术制造了明胶甲基丙烯酰-骨髓间充质干细胞(GelMA-MSCs)支架。该生物链接具有快速凝胶光固化和自发共价交联的功能,可以维持MSCs的高活力,并为促进细胞的相互作用、迁移和增殖提供良好的微环境。体内实验进一步证明,生物3D打印支架能够促进软骨胶原纤维的再生,对兔软骨损伤模型的软骨修复效果显著,这可能为软骨再生系统的精确工程提供一种通用的、通用的策略。
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引用次数: 2
Three-dimensional bioprinting of functional β-islet-like constructs. 功能β-胰岛样结构的三维生物打印。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.v9i2.665
Shahram Parvaneh, Lajos Kemény, Ameneh Ghaffarinia, Reza Yarani, Zoltán Veréb

256Diabetes is an autoimmune disease that ensues when the pancreas does not deliver adequate insulin or when the body cannot react to the existing insulin. Type 1 diabetes is an autoimmune disease defined by continuous high blood sugar levels and insulin deficiency due to β-cell destruction in the islets of Langerhans (pancreatic islets). Long-term complications, such as vascular degeneration, blindness, and renal failure, result from periodic glucose-level fluctuations following exogenous insulin therapy. Nevertheless, the shortage of organ donors and the lifelong dependency on immunosuppressive drugs limit the transplantation of the entire pancreas or pancreas islet, which is the therapy for this disease. Although encapsulating pancreatic islets using multiple hydrogels creates a semi-privileged environment to prevent immune rejection, hypoxia that occurs in the core of the capsules is the main hindrance that should be solved. Bioprinting technology is an innovative process in advanced tissue engineering that allows the arranging of a wide array of cell types, biomaterials, and bioactive factors as a bioink to simulate the native tissue environment for fabricating clinically applicable bioartificial pancreatic islet tissue. Multipotent stem cells have the potential to be a possible solution for donor scarcity and can be a reliable source for generating autograft and allograft functional β-cells or even pancreatic islet-like tissue. The use of supporting cells, such as endothelial cells, regulatory T cells, and mesenchymal stem cells, in the bioprinting of pancreatic islet-like construct could enhance vasculogenesis and regulate immune activity. Moreover, scaffolds bioprinted using biomaterials that can release oxygen postprinting or enhance angiogenesis could increase the function of β-cells and the survival of pancreatic islets, which could represent a promising avenue.

糖尿病是一种自身免疫性疾病,当胰腺不能提供足够的胰岛素或身体不能对现有的胰岛素作出反应时就会发生。1型糖尿病是一种自身免疫性疾病,由朗格汉斯胰岛(胰岛)β细胞破坏引起的持续高血糖水平和胰岛素缺乏所定义。长期并发症,如血管变性、失明和肾功能衰竭,是外源性胰岛素治疗后周期性血糖水平波动的结果。然而,器官供体的缺乏和对免疫抑制药物的终身依赖限制了整个胰腺或胰岛的移植,这是治疗本病的方法。虽然使用多种水凝胶包封胰岛可以创造半特权环境来防止免疫排斥,但发生在胶囊核心的缺氧是应该解决的主要障碍。生物打印技术是先进组织工程中的一项创新技术,它允许将多种细胞类型、生物材料和生物活性因子作为生物链接来模拟天然组织环境,以制造临床适用的生物人工胰岛组织。多能干细胞有可能成为供体稀缺的一种可能解决方案,并且可以作为产生自体和异体移植功能β细胞甚至胰岛样组织的可靠来源。使用支持细胞,如内皮细胞、调节性T细胞和间充质干细胞,在胰岛样结构的生物打印中可以增强血管生成和调节免疫活性。此外,利用生物材料打印的支架可以在打印后释放氧气或促进血管生成,可以提高β细胞的功能和胰岛的存活率,这可能是一个有前景的途径。
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引用次数: 1
Tissue-engineered edible bird's nests (TeeBN). 组织工程可食用鸟巢(TeeBN)。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.691
Yu Liu, Yangyang Liu, Jiayue Liu, Yuwei Li, Jian-Bo Wan, Yiming Niu, Lei Dong, Li Du, Chunming Wang

Edible bird's nests (EBN)-the nests of swiftlet birds harvested from the wild- are high-end healthcare food in East Asia, while their excessive harvesting poses increasing ecological, environmental, and food safety concerns. Here, we report for the first time a tissue-engineering (TE) approach for fabricating EBNs substitutes by integrating the technologies of three-dimensional (3D) printing and live cell culture. The engineered products, tissue-engineered edible bird's nests (TeeBN), comprise two layers. The first is a feeding layer that encapsulates epithelial cells in 3D-printed biocompatible gelation scaffolds. These cells secrete bioactive ingredients, e.g., sialic acid and epidermal growth factors (EGF), recapitulating the natural production of these substances by birds. The second is a receiving layer, consisting of foodgrade natural polymers, e.g., polysaccharides, which mimics the building blocks of natural EBNs while biologically stabilizing the factors released from the feeding layer. In vitro characterizations demonstrate that the feeding layer facilitates 3D cell growth and functions, and the receiving layer (as the end product) contains the necessary nutrients expected from natural EBNs-while without harmful substances commonly detected in natural EBNs. Further, in vivo metabolomics studies in mice indicate that TeeBN showed a similar profile of serum metabolites as natural EBN, reflecting comparable nutritional effects. In summary, we innovatively developed a tissue engineering-based substitute for EBNs with comparable metabolic functions and minimized safety risks, opening a new avenue for producing delicacy food from laboratorial cell culture with 3D printing technology.

食用燕窝(EBN)是一种野生燕窝,是东亚地区的高端保健食品,但它们的过度捕捞引起了越来越多的生态、环境和食品安全问题。在这里,我们首次报道了一种组织工程(TE)方法,通过整合三维(3D)打印和活细胞培养技术来制造EBNs替代品。这种工程产品,组织工程可食用鸟巢(TeeBN),由两层组成。第一个是将上皮细胞包裹在3d打印的生物相容性凝胶支架中的喂养层。这些细胞分泌生物活性成分,如唾液酸和表皮生长因子(EGF),再现了鸟类自然产生这些物质的过程。第二层是接收层,由食品级天然聚合物组成,例如多糖,它模仿天然ebn的构建块,同时生物稳定从喂养层释放的因子。体外表征表明,饲养层促进了3D细胞的生长和功能,而接收层(作为最终产品)含有天然ebn所需的营养物质,同时不含天然ebn中常见的有害物质。此外,小鼠体内代谢组学研究表明,TeeBN显示出与天然EBN相似的血清代谢物特征,反映出类似的营养效果。总之,我们创新地开发了一种基于组织工程的ebn替代品,具有相当的代谢功能和最小的安全风险,为利用3D打印技术从实验室细胞培养中生产美味食品开辟了新的途径。
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引用次数: 0
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International Journal of Bioprinting
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