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3D printing and 3D-printed electronics: Applications and future trends in smart drug delivery devices. 3D打印和3D打印电子产品:智能药物输送设备的应用和未来趋势。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.725
Wai Cheung Ma, Guo Liang Goh, Balasankar Meera Priyadarshini, Wai Yee Yeong

Drug delivery devices which can control the release of drugs on demand allow for improved treatment to a patient. These smart drug delivery devices allow for the release of drugs to be turned on and off as needed, thereby increasing the control over the drug concentration within the patient. The addition of electronics to the smart drug delivery devices increases the functionality and applications of these devices. Through the use of 3D printing and 3D-printed electronics, the customizability and functions of such devices can also be greatly increased. With the development in such technologies, the applications of the devices will be improved. In this review paper, the application of 3D-printed electronics and 3D printing in smart drug delivery devices with electronics as well as the future trends of such applications are covered.

可以根据需要控制药物释放的药物输送装置可以改善对患者的治疗。这些智能药物输送装置允许根据需要打开和关闭药物释放,从而增加对患者体内药物浓度的控制。在智能药物输送设备中添加电子设备增加了这些设备的功能和应用。通过使用3D打印和3D打印电子产品,这些设备的可定制性和功能也可以大大增加。随着这些技术的发展,器件的应用将得到进一步的提高。本文综述了3D打印电子技术和3D打印技术在电子智能给药装置中的应用以及未来的发展趋势。
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引用次数: 1
Design and biomechanical analysis of patientspecific porous tantalum prostheses for knee joint revision surgery. 膝关节翻修手术患者专用多孔钽假体的设计与生物力学分析。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.735
Shilong Mao, Yang Liu, Fuyou Wang, Peng He, Xianzhe Wu, Xingshuang Ma, Yanfeng Luo

Artificial joint revision surgery, as an increasingly common surgery in orthopedics, often requires patient-specific prostheses to repair the bone defect. Porous tantalum is a good candidate due to its excellent abrasion and corrosion resistance and good osteointegration. Combination of 3D printing technology and numerical simulation is a promising strategy to design and prepare patient-specific porous prostheses. However, clinical design cases have rarely been reported, especially from the viewpoint of biomechanical matching with the patient's weight and motion and specific bone tissue. This work reports a clinical case on the design and mechanical analysis of 3D-printed porous tantalum prostheses for the knee revision of an 84-year-old male patient. Particularly, standard cylinders of 3D-printed porous tantalum with different pore size and wire diameters were first fabricated and their compressive mechanical properties were measured for following numerical simulation. Subsequently, patientspecific finite element models for the knee prosthesis and the tibia were constructed from the patient's computed tomography data. The maximum von Mises stress and displacement of the prostheses and tibia and the maximum compressive strain of the tibia were numerically simulated under two loading conditions by using finite element analysis software ABAQUS. Finally, by comparing the simulated data to the biomechanical requirements for the prosthesis and the tibia, a patient-specific porous tantalum knee joint prosthesis with a pore diameter of 600 μm and a wire diameter of 900 μm was determined. The Young's modulus (5719.32 ± 100.61 MPa) and yield strength (172.71 ± 1.67 MPa) of the prosthesis can produce both sufficient mechanical support and biomechanical stimulation to the tibia. This work provides a useful guidance for designing and evaluating a patient-specific porous tantalum prosthesis.

人工关节翻修手术作为骨科中越来越常见的手术,往往需要患者特异性的假体来修复骨缺损。多孔钽具有优异的耐磨损、耐腐蚀性能和良好的骨整合性,是一种很好的候选材料。将3D打印技术与数值模拟相结合是设计和制备患者特异性多孔假体的一种很有前途的策略。然而,临床设计案例很少有报道,特别是从生物力学与患者体重、运动和特定骨组织匹配的角度来看。这项工作报告了一个临床病例的设计和力学分析的3d打印多孔钽假体膝关节翻修的84岁男性患者。首先制备了不同孔径和线径的3d打印多孔钽标准圆柱体,并对其压缩力学性能进行了测量,进行了数值模拟。随后,根据患者的计算机断层扫描数据构建了膝关节假体和胫骨的特定有限元模型。利用有限元分析软件ABAQUS对两种载荷条件下假体和胫骨的最大von Mises应力和位移以及胫骨的最大压缩应变进行数值模拟。最后,通过将模拟数据与假体和胫骨的生物力学要求进行比较,确定了孔径为600 μm、丝径为900 μm的患者专用多孔钽膝关节假体。该假体的杨氏模量(5719.32±100.61 MPa)和屈服强度(172.71±1.67 MPa)可为胫骨提供足够的机械支撑和生物力学刺激。这项工作为设计和评估患者特异性多孔钽假体提供了有用的指导。
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引用次数: 0
3D bioprinting of ultrashort self-assembling peptides to engineer scaffolds with different matrix stiffness for chondrogenesis. 超短自组装肽的3D生物打印技术用于不同基质刚度的软骨形成支架。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.719
Dana M Alhattab, Zainab Khan, Salwa Alshehri, Hepi H Susapto, Charlotte A E Hauser

62Articular cartilage is a nonvascularized and poorly cellularized tissue with a low self-repair capacity. Therefore, damage to this tissue due to trauma or degenerative joint diseases such as osteoarthritis needs a high-end medical intervention. However, such interventions are costly, have limited healing capacity, and could impair patients' quality of life. In this regard, tissue engineering and three-dimensional (3D) bioprinting hold great potential. However, identifying suitable bioinks that are biocompatible, with the desired mechanical stiffness, and can be used under physiological conditions is still a challenge. In this study, we developed two tetrameric self-assembling ultrashort peptide bioinks that are chemically well-defined and can spontaneously form nanofibrous hydrogels under physiological conditions. The printability of the two ultrashort peptides was demonstrated; different shape constructs were printed with high shape fidelity and stability. Furthermore, the developed ultrashort peptide bioinks gave rise to constructs with different mechanical properties that could be used to guide stem cell differentiation toward specific lineages. Both ultrashort peptide bioinks demonstrated high biocompatibility and supported the chondrogenic differentiation of human mesenchymal stem cells. Additionally, the gene expression analysis of differentiated stem cells with the ultrashort peptide bioinks revealed articular cartilage extracellular matrix formation preference. Based on the different mechanical stiffness of the two ultrashort peptide bioinks, they can be used to fabricate cartilage tissue with different cartilaginous zones, including the articular and calcified cartilage zones, which are essential for engineered tissue integration.

62关节软骨是一种无血管化和细胞化不良的组织,自我修复能力低。因此,由于创伤或退行性关节疾病(如骨关节炎)导致的该组织损伤需要高端的医疗干预。然而,这样的干预是昂贵的,有有限的愈合能力,并可能损害患者的生活质量。在这方面,组织工程和三维生物打印具有巨大的潜力。然而,确定合适的生物墨水是生物相容性的,具有所需的机械刚度,并且可以在生理条件下使用仍然是一个挑战。在这项研究中,我们开发了两种四聚体自组装超短肽生物墨水,它们具有良好的化学定义,可以在生理条件下自发形成纳米纤维水凝胶。证实了这两种超短肽的可打印性;打印出不同形状的结构体,具有较高的形状保真度和稳定性。此外,开发的超短肽生物墨水产生了具有不同机械特性的构建物,可用于引导干细胞向特定谱系分化。两种超短肽生物墨水均表现出较高的生物相容性,支持人间充质干细胞成软骨分化。此外,利用超短肽生物墨水对分化干细胞进行基因表达分析,揭示了关节软骨细胞外基质形成的偏好。基于两种超短肽生物墨水不同的机械刚度,它们可以用来制造具有不同软骨带的软骨组织,包括关节软骨带和钙化软骨带,这是工程组织整合所必需的。
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引用次数: 2
Metrological characterization of porosity graded β-Ti21S triply periodic minimal surface cellular structure manufactured by laser powder bed fusion. 激光粉末床熔合制备孔隙度梯度β-Ti21S三周期最小表面胞状结构的计量表征。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.729
Lorena Emanuelli, Raffaele De Biasi, Huijuan Fu, Anton du Plessis, Carlo Lora, Alireza Jam, Matteo Benedetti, Massimo Pellizzari

The design of a functionally graded porous structure (FGPS) for use in prosthetic devices is crucial for meeting both mechanical and biological requirements. One of the most commonly used cellular structures in FGPS is the triply periodic minimal surface (TPMS) structure due to its ability to be defined by implicit equations, which allows for smooth transitions between layers. This study evaluates the feasibility of using a novel β-Ti21S alloy to fabricate TPMS-based FGPS. This beta titanium alloy exhibits low elastic modulus (53 GPa) and good mechanical properties in as-built condition. Two TPMS FGPSs with relative density gradients of 0.17, 0.34, 0.50, 0.66, and 0.83 and unit cell sizes of 2.5 mm and 4 mm were designed and fabricated using laser powder bed fusion (LPBF). The as-manufactured structures were analyzed using scanning electron microscopy (SEM) and X-ray micro-computed tomography (μ-CT), and the results were compared to the design. The analysis revealed that the pore size and ligament thickness were undersized by less than 5%. Compression tests showed that the stabilized elastic modulus was 4.1 GPa for the TPMS with a 2.5 mm unit cell size and 10.7 GPa for the TPMS with a 4 mm unit cell size. A finite element simulation was performed to predict the specimen's elastic properties, and a lumped model based on lattice homogenized properties was proposed and its limitations were explored.

用于假肢装置的功能梯度多孔结构(FGPS)的设计对于满足机械和生物要求至关重要。FGPS中最常用的细胞结构之一是三周期最小表面(TPMS)结构,因为它能够由隐式方程定义,从而允许层之间的平滑过渡。本研究评估了利用新型β-Ti21S合金制备tpms基FGPS的可行性。该β钛合金具有较低的弹性模量(53 GPa)和良好的力学性能。采用激光粉末床熔合(LPBF)技术,设计并制备了相对密度梯度分别为0.17、0.34、0.50、0.66和0.83,晶胞尺寸分别为2.5 mm和4 mm的TPMS FGPSs。利用扫描电子显微镜(SEM)和x射线微计算机断层扫描(μ-CT)对制备的结构进行了分析,并与设计结果进行了比较。分析表明,孔大小和韧带厚度小于5%。压缩试验结果表明,2.5 mm晶胞尺寸的TPMS稳定弹性模量为4.1 GPa, 4 mm晶胞尺寸的TPMS稳定弹性模量为10.7 GPa。采用有限元模拟方法对试件的弹性特性进行了预测,提出了基于点阵均匀化特性的集总模型,并对其局限性进行了探讨。
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引用次数: 1
219Three-dimensional printing as a cutting-edge, versatile and personalizable vascular stent manufacturing procedure: Toward tailor-made medical devices. 三维打印作为一种尖端的、通用的和个性化的血管支架制造工艺:走向量身定制的医疗设备。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.v9i2.664
Fatima Garcia-Villen, Fernando López-Zárraga, Cesar Viseras, Sandra Ruiz-Alonso, Fouad Al-Hakim, Irene Diez-Aldama, Laura Saenz-Del-Burgo, Denis Scaini, Jose Luis Pedraz

Vascular stents (VS) have revolutionized the treatment of cardiovascular diseases, as evidenced by the fact that the implantation of VS in coronary artery disease (CAD) patients has become a routine, easily approachable surgical intervention for the treatment of stenosed blood vessels. Despite the evolution of VS throughout the years, more efficient approaches are still required to address the medical and scientific challenges, especially when it comes to peripheral artery disease (PAD). In this regard, three-dimensional (3D) printing is envisaged as a promising alternative to upgrade VS by optimizing the shape, dimensions and stent backbone (crucial for optimal mechanical properties), making them customizable for each patient and each stenosed lesion. Moreover, the combination of 3D printing with other methods could also upgrade the final device. This review focuses on the most recent studies using 3D printing techniques to produce VS, both by itself and in combination with other techniques. The final aim is to provide an overview of the possibilities and limitations of 3D printing in the manufacturing of VS. Furthermore, the current situation of CAD and PAD pathologies is also addressed, thus highlighting the main weaknesses of the already existing VS and identifying research gaps, possible market niches and future directions.

血管支架(VS)已经彻底改变了心血管疾病的治疗,在冠状动脉疾病(CAD)患者中植入血管支架已成为治疗血管狭窄的常规、易于操作的手术干预措施。尽管多年来VS不断发展,但仍然需要更有效的方法来应对医学和科学挑战,特别是当涉及到外周动脉疾病(PAD)时。在这方面,三维(3D)打印被认为是一种很有前途的替代方案,可以通过优化形状、尺寸和支架骨干(对最佳机械性能至关重要)来升级VS,使其针对每个患者和每个狭窄病变进行定制。此外,3D打印与其他方法的结合也可以使最终设备升级。这篇综述的重点是使用3D打印技术生产VS的最新研究,无论是单独使用还是与其他技术结合使用。最后的目的是概述3D打印在VS制造中的可能性和局限性。此外,CAD和PAD病理的现状也得到了解决,从而突出了现有VS的主要弱点,并确定了研究差距,可能的市场利基和未来的方向。
{"title":"219Three-dimensional printing as a cutting-edge, versatile and personalizable vascular stent manufacturing procedure: Toward tailor-made medical devices.","authors":"Fatima Garcia-Villen,&nbsp;Fernando López-Zárraga,&nbsp;Cesar Viseras,&nbsp;Sandra Ruiz-Alonso,&nbsp;Fouad Al-Hakim,&nbsp;Irene Diez-Aldama,&nbsp;Laura Saenz-Del-Burgo,&nbsp;Denis Scaini,&nbsp;Jose Luis Pedraz","doi":"10.18063/ijb.v9i2.664","DOIUrl":"https://doi.org/10.18063/ijb.v9i2.664","url":null,"abstract":"<p><p>Vascular stents (VS) have revolutionized the treatment of cardiovascular diseases, as evidenced by the fact that the implantation of VS in coronary artery disease (CAD) patients has become a routine, easily approachable surgical intervention for the treatment of stenosed blood vessels. Despite the evolution of VS throughout the years, more efficient approaches are still required to address the medical and scientific challenges, especially when it comes to peripheral artery disease (PAD). In this regard, three-dimensional (3D) printing is envisaged as a promising alternative to upgrade VS by optimizing the shape, dimensions and stent backbone (crucial for optimal mechanical properties), making them customizable for each patient and each stenosed lesion. Moreover, the combination of 3D printing with other methods could also upgrade the final device. This review focuses on the most recent studies using 3D printing techniques to produce VS, both by itself and in combination with other techniques. The final aim is to provide an overview of the possibilities and limitations of 3D printing in the manufacturing of VS. Furthermore, the current situation of CAD and PAD pathologies is also addressed, thus highlighting the main weaknesses of the already existing VS and identifying research gaps, possible market niches and future directions.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 2","pages":"664"},"PeriodicalIF":8.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9f/bb/IJB-9-2-664.PMC10090821.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9672646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Development of polycaprolactone grafts with improved physical properties and body stability using a screw extrusion-type 3D bioprinter. 利用螺旋挤压型3D生物打印机开发具有改善物理性能和体稳定性的聚己内酯移植物。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.v9i2.652
Su Hee Kim, Se Jun Park, Bin Xu, Jae Hyup Lee, Sang Jin An, Misun Cha

Three-dimensional (3D) bioprinter including screw extruder was developed, and the polycaprolactone (PCL) grafts fabricated by screw-type and pneumatic pressure-type bioprinters were comparatively evaluated. The density and tensile strength of the single layers printed by the screw-type were 14.07% and 34.76% higher, respectively, than those of the single layers produced by the pneumatic pressure-type. The adhesive force, tensile strength, and bending strength of the PCL grafts printed by the screw-type bioprinter were 2.72 times, 29.89%, and 67.76% higher, respectively, than those of the PCL grafts prepared by the pneumatic pressure-type bioprinter. By evaluating the consistency with the original image of the PCL grafts, we found that it had a value of about 98.35%. The layer width of the printing structure was 485.2 ± 0.004919 μm, which was 99.5% to 101.8% compared to the set value (500 μm), indicating high accuracy and uniformity. The printed graft had no cytotoxicity, and there were no impurities in the extract test. In the in vivo studies, the tensile strength of the sample 12 months after implantation was reduced by 50.37% and 85.43% compared to the initial point of the sample printed by the screw-type and the pneumatic pressure-type, respectively. Through observing the fractures of the samples at 9- and 12-month samples, we found that the PCL grafts prepared by the screw-type had better in vivo stability. Therefore, the printing system developed in this study can be used as a treatment for regenerative medicine.

研制了包括螺杆挤出机在内的三维生物打印机,并对螺杆式和气压式生物打印机制备的聚己内酯(PCL)接枝材料进行了比较评价。螺杆式打印的单层密度和拉伸强度分别比气压式打印的单层密度和拉伸强度高14.07%和34.76%。螺杆式生物打印机打印的PCL接枝的粘合力、抗拉强度和抗弯强度分别比气压式生物打印机打印的PCL接枝高2.72倍、29.89%和67.76%。通过与PCL移植物原始图像的一致性评估,我们发现其价值约为98.35%。打印结构的层宽为485.2±0.004919 μm,比设定值(500 μm)提高了99.5% ~ 101.8%,具有较高的精度和均匀性。打印的移植物无细胞毒性,提取液试验无杂质。在体内研究中,与螺杆式和气压式打印的样品初始点相比,植入12个月后样品的抗拉强度分别降低了50.37%和85.43%。通过观察9个月和12个月标本的骨折情况,我们发现螺钉式制备的PCL移植物具有更好的体内稳定性。因此,本研究开发的打印系统可以作为再生医学的一种治疗方法。
{"title":"Development of polycaprolactone grafts with improved physical properties and body stability using a screw extrusion-type 3D bioprinter.","authors":"Su Hee Kim,&nbsp;Se Jun Park,&nbsp;Bin Xu,&nbsp;Jae Hyup Lee,&nbsp;Sang Jin An,&nbsp;Misun Cha","doi":"10.18063/ijb.v9i2.652","DOIUrl":"https://doi.org/10.18063/ijb.v9i2.652","url":null,"abstract":"<p><p>Three-dimensional (3D) bioprinter including screw extruder was developed, and the polycaprolactone (PCL) grafts fabricated by screw-type and pneumatic pressure-type bioprinters were comparatively evaluated. The density and tensile strength of the single layers printed by the screw-type were 14.07% and 34.76% higher, respectively, than those of the single layers produced by the pneumatic pressure-type. The adhesive force, tensile strength, and bending strength of the PCL grafts printed by the screw-type bioprinter were 2.72 times, 29.89%, and 67.76% higher, respectively, than those of the PCL grafts prepared by the pneumatic pressure-type bioprinter. By evaluating the consistency with the original image of the PCL grafts, we found that it had a value of about 98.35%. The layer width of the printing structure was 485.2 ± 0.004919 μm, which was 99.5% to 101.8% compared to the set value (500 μm), indicating high accuracy and uniformity. The printed graft had no cytotoxicity, and there were no impurities in the extract test. In the in vivo studies, the tensile strength of the sample 12 months after implantation was reduced by 50.37% and 85.43% compared to the initial point of the sample printed by the screw-type and the pneumatic pressure-type, respectively. Through observing the fractures of the samples at 9- and 12-month samples, we found that the PCL grafts prepared by the screw-type had better in vivo stability. Therefore, the printing system developed in this study can be used as a treatment for regenerative medicine.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 2","pages":"652"},"PeriodicalIF":8.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/93/74/IJB-9-2-652.PMC10090531.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9672648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Commercial articulated collaborative in situ 3D bioprinter for skin wound healing. 用于皮肤伤口愈合的商业关节协作原位3D生物打印机。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.v9i2.675
Aleksandr A Levin, Pavel A Karalkin, Elizaveta V Koudan, Fedor S Senatov, Vladislav A Parfenov, Vladislav A Lvov, Stanislav V Petrov, Frederico D A S Pereira, Alexey V Kovalev, Egor O Osidak, Sergey P Domogatsky, Natalya E Manturova, Vladimir A Kasyanov, Natalia S Sergeeva, Vadim L Zorin, Yusef D Khesuani, Vladimir A Mironov

In situ bioprinting is one of the most clinically relevant techniques in the emerging bioprinting technology because it could be performed directly on the human body in the operating room and it does not require bioreactors for post-printing tissue maturation. However, commercial in situ bioprinters are still not available on the market. In this study, we demonstrated the benefit of the originally developed first commercial articulated collaborative in situ bioprinter for the treatment of full-thickness wounds in rat and porcine models. We used an articulated and collaborative robotic arm from company KUKA and developed original printhead and correspondence software enabling in situ bioprinting on curve and moving surfaces. The results of in vitro and in vivo experiments show that in situ bioprinting of bioink induces a strong hydrogel adhesion and enables printing on curved surfaces of wet tissues with a high level of fidelity. The in situ bioprinter was convenient to use in the operating room. Additional in vitro experiments (in vitro collagen contraction assay and in vitro 3D angiogenesis assay) and histological analyses demonstrated that in situ bioprinting improves the quality of wound healing in rat and porcine skin wounds. The absence of interference with the normal process of wound healing and even certain improvement in the dynamics of this process strongly suggests that in situ bioprinting could be used as a novel therapeutic modality in wound healing.

原位生物打印技术是新兴的生物打印技术中最具临床意义的技术之一,因为它可以在手术室中直接在人体上进行,并且不需要生物反应器进行打印后的组织成熟。然而,市场上仍然没有商业化的原位生物打印机。在这项研究中,我们展示了最初开发的第一个商业铰接式协作原位生物打印机在大鼠和猪模型中治疗全层伤口的益处。我们使用了KUKA公司的铰接式协作机械臂,并开发了原始打印头和通信软件,可以在曲线和移动表面上进行原位生物打印。体外和体内实验结果表明,生物墨水的原位生物打印具有很强的水凝胶附着力,能够在湿组织的曲面上进行高保真度的打印。原位生物打印机在手术室使用方便。另外的体外实验(体外胶原收缩实验和体外3D血管生成实验)和组织学分析表明,原位生物打印提高了大鼠和猪皮肤伤口愈合的质量。没有对正常伤口愈合过程的干扰,甚至在一定程度上改善了这一过程的动力学,这强烈表明原位生物打印可以作为一种新的伤口愈合治疗方式。
{"title":"Commercial articulated collaborative <i>in situ</i> 3D bioprinter for skin wound healing.","authors":"Aleksandr A Levin,&nbsp;Pavel A Karalkin,&nbsp;Elizaveta V Koudan,&nbsp;Fedor S Senatov,&nbsp;Vladislav A Parfenov,&nbsp;Vladislav A Lvov,&nbsp;Stanislav V Petrov,&nbsp;Frederico D A S Pereira,&nbsp;Alexey V Kovalev,&nbsp;Egor O Osidak,&nbsp;Sergey P Domogatsky,&nbsp;Natalya E Manturova,&nbsp;Vladimir A Kasyanov,&nbsp;Natalia S Sergeeva,&nbsp;Vadim L Zorin,&nbsp;Yusef D Khesuani,&nbsp;Vladimir A Mironov","doi":"10.18063/ijb.v9i2.675","DOIUrl":"https://doi.org/10.18063/ijb.v9i2.675","url":null,"abstract":"<p><p><i>In situ</i> bioprinting is one of the most clinically relevant techniques in the emerging bioprinting technology because it could be performed directly on the human body in the operating room and it does not require bioreactors for post-printing tissue maturation. However, commercial <i>in situ</i> bioprinters are still not available on the market. In this study, we demonstrated the benefit of the originally developed first commercial articulated collaborative <i>in situ</i> bioprinter for the treatment of full-thickness wounds in rat and porcine models. We used an articulated and collaborative robotic arm from company KUKA and developed original printhead and correspondence software enabling <i>in situ</i> bioprinting on curve and moving surfaces. The results of <i>in vitro</i> and <i>in vivo</i> experiments show that <i>in situ</i> bioprinting of bioink induces a strong hydrogel adhesion and enables printing on curved surfaces of wet tissues with a high level of fidelity. The <i>in situ</i> bioprinter was convenient to use in the operating room. Additional <i>in vitro</i> experiments (<i>in vitro</i> collagen contraction assay and <i>in vitro</i> 3D angiogenesis assay) and histological analyses demonstrated that <i>in situ</i> bioprinting improves the quality of wound healing in rat and porcine skin wounds. The absence of interference with the normal process of wound healing and even certain improvement in the dynamics of this process strongly suggests that <i>in situ</i> bioprinting could be used as a novel therapeutic modality in wound healing.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 2","pages":"675"},"PeriodicalIF":8.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e8/7c/IJB-9-2-675.PMC10090815.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9687468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Error assessment and correction for extrusion-based bioprinting using computer vision method. 基于计算机视觉的挤出生物打印误差评估与校正。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.v9i1.644
Changxi Liu, Chengliang Yang, Jia Liu, Yujin Tang, Zhengjie Lin, Long Li, Hai Liang, Weijie Lu, Liqiang Wang

299Bioprinting offers a new approach to addressing the organ shortage crisis. Despite recent technological advances, insufficient printing resolution continues to be one of the reasons that impede the development of bioprinting. Normally, machine axes movement cannot be reliably used to predict material placement, and the printing path tends to deviate from the predetermined designed reference trajectory in varying degrees. Therefore, a computer vision-based method was proposed in this study to correct trajectory deviation and improve printing accuracy. The image algorithm calculated the deviation between the printed trajectory and the reference trajectory to generate an error vector. Furthermore, the axes trajectory was modified according to the normal vector approach in the second printing to compensate for the deviation error. The highest correction efficiency that could be achieved was 91%. More significantly, we discovered that the correction results, for the first time, were in a normal distribution instead of a random distribution.

生物打印为解决器官短缺危机提供了一种新的途径。尽管最近的技术进步,打印分辨率不足仍然是阻碍生物打印发展的原因之一。通常情况下,机械轴的运动不能可靠地预测材料的放置位置,并且打印路径往往不同程度地偏离预定设计的参考轨迹。因此,本研究提出了一种基于计算机视觉的方法来纠正轨迹偏差,提高打印精度。图像算法计算打印轨迹与参考轨迹之间的偏差,生成误差向量。在二次打印时,根据法向量法对轴轨迹进行修正,以补偿偏差误差。校正效率最高可达91%。更重要的是,我们第一次发现校正结果是正态分布,而不是随机分布。
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引用次数: 1
Toward better drug development: Three-dimensional bioprinting in toxicological research. 迈向更好的药物开发:毒理学研究中的三维生物打印。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.v9i2.663
Diána Szűcs, Zsolt Fekete, Melinda Guba, Lajos Kemény, Katalin Jemnitz, Emese Kis, Zoltán Veréb

The importance of three-dimensional (3D) models in pharmacological tests and personalized therapies is significant. These models allow us to gain insight into the cell response during drug absorption, distribution, metabolism, and elimination in an organ-like system and are suitable for toxicological testing. In personalized and regenerative medicine, the precise characterization of artificial tissues or drug metabolism processes is more than crucial to gain the safest and the most effective treatment for the patients. Using these 3D cell cultures derived directly from patient, such as spheroids, organoids, and bioprinted structures, allows for testing drugs before administration to the patient. These methods allow us to select the most appropriate drug for the patient. Moreover, they provide chance for better recovery of patients, since time is not wasted during therapy switching. These models could be used in applied and basic research as well, because their response to treatments is quite similar to that of the native tissue. Furthermore, they may replace animal models in the future because these methods are cheaper and can avoid interspecies differences. This review puts a spotlight on this dynamically evolving area and its application in toxicological testing.

三维(3D)模型在药理学试验和个性化治疗中的重要性是显著的。这些模型使我们能够深入了解类器官系统中药物吸收、分布、代谢和消除过程中的细胞反应,并适用于毒理学测试。在个性化和再生医学中,人工组织或药物代谢过程的精确表征对于为患者获得最安全和最有效的治疗至关重要。使用这些直接来源于患者的3D细胞培养物,如球体、类器官和生物打印结构,可以在给药之前对药物进行测试。这些方法使我们能够为病人选择最合适的药物。此外,它们为患者提供了更好的康复机会,因为在治疗转换过程中不会浪费时间。这些模型可以用于应用和基础研究,因为它们对治疗的反应与天然组织非常相似。此外,它们可能在未来取代动物模型,因为这些方法更便宜,并且可以避免物种间的差异。本文就这一动态发展的领域及其在毒理学检测中的应用作一综述。
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引用次数: 2
Preparation and characterization of 3D-printed antibacterial hydrogel with benzyl isothiocyanate. 异硫氰酸苄酯3d打印抗菌水凝胶的制备与表征。
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.18063/ijb.v9i2.671
Yunxia Liang, Bimal Chitrakar, Zhenbin Liu, Xujia Ming, Dan Xu, Haizhen Mo, Chunyang Shi, Xiaolin Zhu, Liangbin Hu, Hongbo Li

Benzyl isothiocyanate (BITC) is an isothiocyanate of plant origin, especially the mustard family, which has good antibacterial properties. However, its applications are challenging due to its poor water solubility and chemical instability. We used food hydrocolloids, including xanthan gum, locust bean gum, konjac glucomannan, and carrageenan as three-dimensional (3D)-printing food ink base and successfully prepared 3D-printed BITC antibacterial hydrogel (BITC-XLKC-Gel). The characterization and fabrication procedure of BITC-XLKC-Gel was studied. The results show that BITC-XLKC-Gel hydrogel has better mechanical properties by low-field nuclear magnetic resonance (LF-NMR), mechanical properties, and rheometer analysis. The strain rate of BITC-XLKC-Gel hydrogel is 76.5%, which is better than that of human skin. Scanning electron microscope (SEM) analysis showed that BITC-XLKC-Gel has uniform pore size and provides a good carrier environment for BITC carriers. In addition, BITC-XLKC-Gel has good 3D-printing performance, and 3D printing can be used for customizing patterns. Finally, inhibition zone analysis showed that the BITC-XLKC-Gel added with 0.6% BITC had strong antibacterial activity against Staphylococcus aureus and the BITC-XLKC-Gel added with 0.4% BITC had strong antibacterial activity against Escherichia coli. Antibacterial wound dressing has always been considered essential in burn wound healing. In experiments that simulated burn infection, BITC-XLKC-Gel showed good antimicrobial activity against methicillin-resistant S. aureus. BITC-XLKC-Gel is a good 3D-printing food ink attributed to strong plasticity, high safety profile, and good antibacterial performance and has great application prospects.

异硫氰酸苄酯(Benzyl isothiocyanate, BITC)是一种植物来源的异硫氰酸酯,尤其是芥菜科植物,具有良好的抗菌性能。然而,由于其水溶性差和化学不稳定性,其应用具有挑战性。我们以黄原胶、槐豆胶、魔芋葡甘露聚糖、卡拉胶等食品水胶体为三维(3D)打印食品墨基,成功制备了3D打印BITC抗菌水凝胶(BITC- xlkc - gel)。研究了bitc - xlkc凝胶的表征和制备工艺。低场核磁共振(LF-NMR)、力学性能和流变仪分析结果表明,BITC-XLKC-Gel水凝胶具有较好的力学性能。BITC-XLKC-Gel水凝胶的应变率为76.5%,优于人体皮肤。扫描电镜(SEM)分析表明,BITC- xlkc - gel具有均匀的孔径,为BITC载体提供了良好的载体环境。此外,BITC-XLKC-Gel具有良好的3D打印性能,3D打印可用于定制图案。最后,抑菌区分析表明,添加0.6% BITC的BITC- xlkc - gel对金黄色葡萄球菌具有较强的抑菌活性,添加0.4% BITC的BITC- xlkc - gel对大肠杆菌具有较强的抑菌活性。抗菌创面敷料在烧伤创面愈合中一直被认为是必不可少的。在模拟烧伤感染的实验中,BITC-XLKC-Gel对耐甲氧西林金黄色葡萄球菌表现出良好的抗菌活性。BITC-XLKC-Gel是一种良好的3d打印食品油墨,具有可塑性强、安全性高、抗菌性能好等特点,具有广阔的应用前景。
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引用次数: 3
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International Journal of Bioprinting
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