Dong‐liang Wu, Shumin Pang, Viola Röhrs, Johanna Berg, A. S. Ali, Yikun Mei, Mathias Ziersch, Beatrice Tolksdorf, Jens Kurreck
The bioink mixing process is highly relevant to the bioink quality, which is the basis for reproducible extrusion-based three-dimensional (3D) bioprinting (EBB). Currently, most bioinks mixed by skilled human operators show variations in terms of cell homogeneity and biological properties as well as other properties. For preparation of many types of bioinks, striking the balance between homogeneity and cell viability remains a major challenge. This study investigates the relationship between bioink homogeneity and mixing parameters, particularly mixing speed and number of exchanges, utilizing a customized automated device. We found that up to a certain point, increasing the rate of mixing led to a better distribution of cells within the bioink, but beyond that point, there was a detrimental effect on cell viability. In contrast, the mixing number had less impact on the physiological properties of the cells in the bioink. Furthermore, a comparison between skilled human and machine bioink mixing revealed that the machine consistently provided better outcomes in terms of bioink homogeneity, cell distribution, and cell viability, highlighting the advantages and importance of standardizing the bioink mixing process. The methodology and approaches in this study can improve the reproducibility and reliability of EBB bioink and may thereby advance the field of 3D bioprinting in various applications.
{"title":"Man vs. machine: Automated bioink mixing device improves reliability and reproducibility of bioprinting results compared to human operators","authors":"Dong‐liang Wu, Shumin Pang, Viola Röhrs, Johanna Berg, A. S. Ali, Yikun Mei, Mathias Ziersch, Beatrice Tolksdorf, Jens Kurreck","doi":"10.36922/ijb.1974","DOIUrl":"https://doi.org/10.36922/ijb.1974","url":null,"abstract":"The bioink mixing process is highly relevant to the bioink quality, which is the basis for reproducible extrusion-based three-dimensional (3D) bioprinting (EBB). Currently, most bioinks mixed by skilled human operators show variations in terms of cell homogeneity and biological properties as well as other properties. For preparation of many types of bioinks, striking the balance between homogeneity and cell viability remains a major challenge. This study investigates the relationship between bioink homogeneity and mixing parameters, particularly mixing speed and number of exchanges, utilizing a customized automated device. We found that up to a certain point, increasing the rate of mixing led to a better distribution of cells within the bioink, but beyond that point, there was a detrimental effect on cell viability. In contrast, the mixing number had less impact on the physiological properties of the cells in the bioink. Furthermore, a comparison between skilled human and machine bioink mixing revealed that the machine consistently provided better outcomes in terms of bioink homogeneity, cell distribution, and cell viability, highlighting the advantages and importance of standardizing the bioink mixing process. The methodology and approaches in this study can improve the reproducibility and reliability of EBB bioink and may thereby advance the field of 3D bioprinting in various applications.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140458466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Neurotrauma mainly includes brain injury, spinal cord injury, and peripheral nerve injury, which are characterized by high morbidity and disability rates, and involve costly treatments. Currently, various strategies have been applied for the treatment of neurotrauma, but their efficacy is unsatisfactory. New effective strategies are needed to be developed to promote recovery after neurotrauma. In recent years, three-dimensional (3D) printing technology has been used to manufacture customized and complex constructs in tissue engineering applications, exhibiting great potential in repairing nervous system injuries. In this review, we introduce the principles and advantages of 3D printing and 3D bioprinting technologies that have been applied to repair injured nervous system. In particular, we summarize the current strategies in the aspects of biomaterials, physical stimulation, bioactive substances, cell transplantation, and their combination that have been considered in fabricating 3D-printed devices for neurotrauma treatment. Additionally, the challenges and prospects of 3D printing for neurotrauma treatment were also presented.
{"title":"The promising applications of 3D printing technology in neurotrauma","authors":"Wenbo He, Chongxi Xu, Wenbi Wu, Yuchen Chen, Jingxuan Hou, Zhouhaoran Chen, Jianguo Xu, Maling Gou, Yu Hu","doi":"10.36922/ijb.2311","DOIUrl":"https://doi.org/10.36922/ijb.2311","url":null,"abstract":"Neurotrauma mainly includes brain injury, spinal cord injury, and peripheral nerve injury, which are characterized by high morbidity and disability rates, and involve costly treatments. Currently, various strategies have been applied for the treatment of neurotrauma, but their efficacy is unsatisfactory. New effective strategies are needed to be developed to promote recovery after neurotrauma. In recent years, three-dimensional (3D) printing technology has been used to manufacture customized and complex constructs in tissue engineering applications, exhibiting great potential in repairing nervous system injuries. In this review, we introduce the principles and advantages of 3D printing and 3D bioprinting technologies that have been applied to repair injured nervous system. In particular, we summarize the current strategies in the aspects of biomaterials, physical stimulation, bioactive substances, cell transplantation, and their combination that have been considered in fabricating 3D-printed devices for neurotrauma treatment. Additionally, the challenges and prospects of 3D printing for neurotrauma treatment were also presented.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140458192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bioprinting is a novel technique with a wide range of potential uses, including the fabrication of functioning tissue constructs for use in the biomedical sectors. It is a revolutionary method for high-throughput manufacturing that automates fine control over manufactured structures. Bioink refers to the solution of biomaterials usually encapsulating cells used in the bioprinting process; this bioink often encapsulates the appropriate cell types. In order to create the ultimate architecture, this bioink should solidify during or shortly after bioprinting. Bioinks can be developed from either all-natural or all-synthetic biomaterials, or a blend of the two. Cell aggregation can occasionally be used as a bioink without addition of any biomaterials, in bioprinting process. To bioprint functional tissues and organs, an optimal bioink should possess mechanical, rheological, and biological characteristics mimicking those of the target tissues. For attaining physicomechanical properties, anisotropic fillers are commonly added in bioink formulations. In this review, we provide an in-depth discussion of various anisotropic fillers used in bioprinting and their fabrication techniques, and outline their multifunctional applicability in biomedical and environmental areas. Given the steady growth of bioprinting market, we also present the global scenario of the bioprinting market and their techno-commercial orientations.
{"title":"3D bioprinting of anisotropic filler-reinforced polymer nanocomposites: Synthesis, assembly, and multifunctional applications","authors":"Yuan Wu, Sayan Ganguly, X. Tang","doi":"10.36922/ijb.1637","DOIUrl":"https://doi.org/10.36922/ijb.1637","url":null,"abstract":"Bioprinting is a novel technique with a wide range of potential uses, including the fabrication of functioning tissue constructs for use in the biomedical sectors. It is a revolutionary method for high-throughput manufacturing that automates fine control over manufactured structures. Bioink refers to the solution of biomaterials usually encapsulating cells used in the bioprinting process; this bioink often encapsulates the appropriate cell types. In order to create the ultimate architecture, this bioink should solidify during or shortly after bioprinting. Bioinks can be developed from either all-natural or all-synthetic biomaterials, or a blend of the two. Cell aggregation can occasionally be used as a bioink without addition of any biomaterials, in bioprinting process. To bioprint functional tissues and organs, an optimal bioink should possess mechanical, rheological, and biological characteristics mimicking those of the target tissues. For attaining physicomechanical properties, anisotropic fillers are commonly added in bioink formulations. In this review, we provide an in-depth discussion of various anisotropic fillers used in bioprinting and their fabrication techniques, and outline their multifunctional applicability in biomedical and environmental areas. Given the steady growth of bioprinting market, we also present the global scenario of the bioprinting market and their techno-commercial orientations.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139864775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As a bridge that transmits airborne sound signals to the auditory receptors of the inner ear, the eardrum and ossicular chain of the middle ear convert sound through two types of conversions: gas–solid (airborne sound signal–eardrum and ossicular chain) and solid–liquid (eardrum and ossicular chain–internal and external lymphatic fluid in the cochlea). This process concentrates and amplifies the sound to the inner ear through the lever principle structure formed by the three ossicles. However, diseases, hereditary factors, or trauma can reduce the sound transmission function of the middle ear. The effectiveness of middle ear replacement prostheses depends on their vibration response to the human auditory perception frequency, from the eardrum to the stapes plate. This response is influenced by the materials, geometry, and design of the replacement prosthesis and eardrum. This study explores the effects of different materials on hearing after artificial ossicular replacement. Usually, human temporal bone models are used for testing and validating numerical results. However, obtaining specimens from living humans is not always feasible. Therefore, we used three-dimensional printing technology to build a model of the middle ear to test the ossicular bone. Titanium alloy TC4, stainless steel 316L, and composite HA/PCL are chosen as materials for ossicular replacement. Using finite element analysis and an in vitro verification experiment, individual replacements of the ossicles and three bone material replacements were conducted for frequency response analysis. The combination of the malleus made of TC4, the incus made of TC4, and the stapes made of HA/PCL were found to bear higher resemblance to a real normal ear ossicular model.
{"title":"Frequency response analysis and in vitro verification of 3D-printed ossicular replacement materials","authors":"Jingbin Hao, Yin Zhu, Ding Shen, Md Thowfiqure Rahman, Yinxin Kou, Houguang Liu","doi":"10.36922/ijb.2040","DOIUrl":"https://doi.org/10.36922/ijb.2040","url":null,"abstract":"As a bridge that transmits airborne sound signals to the auditory receptors of the inner ear, the eardrum and ossicular chain of the middle ear convert sound through two types of conversions: gas–solid (airborne sound signal–eardrum and ossicular chain) and solid–liquid (eardrum and ossicular chain–internal and external lymphatic fluid in the cochlea). This process concentrates and amplifies the sound to the inner ear through the lever principle structure formed by the three ossicles. However, diseases, hereditary factors, or trauma can reduce the sound transmission function of the middle ear. The effectiveness of middle ear replacement prostheses depends on their vibration response to the human auditory perception frequency, from the eardrum to the stapes plate. This response is influenced by the materials, geometry, and design of the replacement prosthesis and eardrum. This study explores the effects of different materials on hearing after artificial ossicular replacement. Usually, human temporal bone models are used for testing and validating numerical results. However, obtaining specimens from living humans is not always feasible. Therefore, we used three-dimensional printing technology to build a model of the middle ear to test the ossicular bone. Titanium alloy TC4, stainless steel 316L, and composite HA/PCL are chosen as materials for ossicular replacement. Using finite element analysis and an in vitro verification experiment, individual replacements of the ossicles and three bone material replacements were conducted for frequency response analysis. The combination of the malleus made of TC4, the incus made of TC4, and the stapes made of HA/PCL were found to bear higher resemblance to a real normal ear ossicular model.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139865843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Although inkjet-based bioprinting enables precise drop-on-demand cell deposition within three-dimensional (3D) tissue constructs and facilitates critical cell–cell and cell–matrix interactions, it faces challenges such as poor cell homogeneity and low cell viability. To date, there is a lack of comprehensive review papers addressing the optimization of cell deposition in inkjet-based bioprinting. This review aims to fill that gap by providing an overview of various critical aspects in bioprinting, ranging from bio-ink properties to the impact of printed droplets. The bio-ink section begins by exploring how cells influence the physical properties of bio-inks and emphasizes the significance of achieving cell homogeneity within bio-inks to ensure consistent and reliable printing. The discussion then delves into inkjet-based printing chambers (thermal and piezoelectric), the effect of shear stress on printed cells, droplet formation dynamics, the influence of polymer-based and cell-laden droplets on the underlying substrate surface, and the dynamics of droplet impact. Beyond droplet formation and impact, the review highlights the importance of biophysical and biological cues within 3D hydrogel matrices for cell proliferation and differentiation. Finally, the paper highlights current and potential applications, with a specific focus on skin and lung tissue engineering using inkjet-based bioprinting techniques, and provides insights into the emerging role of machine learning in optimizing the cell deposition process for inkjet-based bioprinting.
{"title":"Optimizing cell deposition for inkjet-based bioprinting","authors":"Wei Long Ng, V. Shkolnikov","doi":"10.36922/ijb.2135","DOIUrl":"https://doi.org/10.36922/ijb.2135","url":null,"abstract":"Although inkjet-based bioprinting enables precise drop-on-demand cell deposition within three-dimensional (3D) tissue constructs and facilitates critical cell–cell and cell–matrix interactions, it faces challenges such as poor cell homogeneity and low cell viability. To date, there is a lack of comprehensive review papers addressing the optimization of cell deposition in inkjet-based bioprinting. This review aims to fill that gap by providing an overview of various critical aspects in bioprinting, ranging from bio-ink properties to the impact of printed droplets. The bio-ink section begins by exploring how cells influence the physical properties of bio-inks and emphasizes the significance of achieving cell homogeneity within bio-inks to ensure consistent and reliable printing. The discussion then delves into inkjet-based printing chambers (thermal and piezoelectric), the effect of shear stress on printed cells, droplet formation dynamics, the influence of polymer-based and cell-laden droplets on the underlying substrate surface, and the dynamics of droplet impact. Beyond droplet formation and impact, the review highlights the importance of biophysical and biological cues within 3D hydrogel matrices for cell proliferation and differentiation. Finally, the paper highlights current and potential applications, with a specific focus on skin and lung tissue engineering using inkjet-based bioprinting techniques, and provides insights into the emerging role of machine learning in optimizing the cell deposition process for inkjet-based bioprinting.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139805563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As a bridge that transmits airborne sound signals to the auditory receptors of the inner ear, the eardrum and ossicular chain of the middle ear convert sound through two types of conversions: gas–solid (airborne sound signal–eardrum and ossicular chain) and solid–liquid (eardrum and ossicular chain–internal and external lymphatic fluid in the cochlea). This process concentrates and amplifies the sound to the inner ear through the lever principle structure formed by the three ossicles. However, diseases, hereditary factors, or trauma can reduce the sound transmission function of the middle ear. The effectiveness of middle ear replacement prostheses depends on their vibration response to the human auditory perception frequency, from the eardrum to the stapes plate. This response is influenced by the materials, geometry, and design of the replacement prosthesis and eardrum. This study explores the effects of different materials on hearing after artificial ossicular replacement. Usually, human temporal bone models are used for testing and validating numerical results. However, obtaining specimens from living humans is not always feasible. Therefore, we used three-dimensional printing technology to build a model of the middle ear to test the ossicular bone. Titanium alloy TC4, stainless steel 316L, and composite HA/PCL are chosen as materials for ossicular replacement. Using finite element analysis and an in vitro verification experiment, individual replacements of the ossicles and three bone material replacements were conducted for frequency response analysis. The combination of the malleus made of TC4, the incus made of TC4, and the stapes made of HA/PCL were found to bear higher resemblance to a real normal ear ossicular model.
{"title":"Frequency response analysis and in vitro verification of 3D-printed ossicular replacement materials","authors":"Jingbin Hao, Yin Zhu, Ding Shen, Md Thowfiqure Rahman, Yinxin Kou, Houguang Liu","doi":"10.36922/ijb.2040","DOIUrl":"https://doi.org/10.36922/ijb.2040","url":null,"abstract":"As a bridge that transmits airborne sound signals to the auditory receptors of the inner ear, the eardrum and ossicular chain of the middle ear convert sound through two types of conversions: gas–solid (airborne sound signal–eardrum and ossicular chain) and solid–liquid (eardrum and ossicular chain–internal and external lymphatic fluid in the cochlea). This process concentrates and amplifies the sound to the inner ear through the lever principle structure formed by the three ossicles. However, diseases, hereditary factors, or trauma can reduce the sound transmission function of the middle ear. The effectiveness of middle ear replacement prostheses depends on their vibration response to the human auditory perception frequency, from the eardrum to the stapes plate. This response is influenced by the materials, geometry, and design of the replacement prosthesis and eardrum. This study explores the effects of different materials on hearing after artificial ossicular replacement. Usually, human temporal bone models are used for testing and validating numerical results. However, obtaining specimens from living humans is not always feasible. Therefore, we used three-dimensional printing technology to build a model of the middle ear to test the ossicular bone. Titanium alloy TC4, stainless steel 316L, and composite HA/PCL are chosen as materials for ossicular replacement. Using finite element analysis and an in vitro verification experiment, individual replacements of the ossicles and three bone material replacements were conducted for frequency response analysis. The combination of the malleus made of TC4, the incus made of TC4, and the stapes made of HA/PCL were found to bear higher resemblance to a real normal ear ossicular model.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139805895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nazi Zhou, Shunyao Zhu, Xinlin Wei, Xueyuan Liao, Yu Wang, Yue Xu, Liyun Bai, Haoyuan Wan, Li Liu, Jiumeng Zhang, Ling Zeng, Jie Tao, Rui Liu
Infections to dental pulp commonly result in pulpitis and pulp necrosis, and surgical removal of the infected tissues is the only therapeutic approach. Dental pulp injury remains a challenging medical issue due to the limited regenerative capability of dental pulp. In this work, a dental pulp guidance construct (DPGC) with the instructive niche was bioprinted to mimic native teeth for dentin and neovascular-like structure reconstruction. GelMA-Dextran aqueous emulsion was used as an ink for in situ printing of porous DPGC to induce predominant nuclear localization of Yes-associated protein (YAP) in the encapsulated dental pulp stem cells (DPSCs) and enhance their stemness properties. Furthermore, the DPSCs encapsulated in DPGC with microporous structures exhibited enhanced viability, migration, and spreading. Meanwhile, we found that DPGC could promote capillary tube formation and induce neurogenesis. In a mouse subcutaneous implant model, the DPGC consisted of porous structures, such as odontoblasts and newly formed vascular structures, that mimic dental pulp characteristics. This study demonstrated a new strategy to design DPGC with instructive niche for dental pulp regeneration, presenting a potential treatment alternative to root canal therapy.
{"title":"3D-bioprinted hydrogels with instructive niches for dental pulp regeneration","authors":"Nazi Zhou, Shunyao Zhu, Xinlin Wei, Xueyuan Liao, Yu Wang, Yue Xu, Liyun Bai, Haoyuan Wan, Li Liu, Jiumeng Zhang, Ling Zeng, Jie Tao, Rui Liu","doi":"10.36922/ijb.1790","DOIUrl":"https://doi.org/10.36922/ijb.1790","url":null,"abstract":"Infections to dental pulp commonly result in pulpitis and pulp necrosis, and surgical removal of the infected tissues is the only therapeutic approach. Dental pulp injury remains a challenging medical issue due to the limited regenerative capability of dental pulp. In this work, a dental pulp guidance construct (DPGC) with the instructive niche was bioprinted to mimic native teeth for dentin and neovascular-like structure reconstruction. GelMA-Dextran aqueous emulsion was used as an ink for in situ printing of porous DPGC to induce predominant nuclear localization of Yes-associated protein (YAP) in the encapsulated dental pulp stem cells (DPSCs) and enhance their stemness properties. Furthermore, the DPSCs encapsulated in DPGC with microporous structures exhibited enhanced viability, migration, and spreading. Meanwhile, we found that DPGC could promote capillary tube formation and induce neurogenesis. In a mouse subcutaneous implant model, the DPGC consisted of porous structures, such as odontoblasts and newly formed vascular structures, that mimic dental pulp characteristics. This study demonstrated a new strategy to design DPGC with instructive niche for dental pulp regeneration, presenting a potential treatment alternative to root canal therapy.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139864178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Although inkjet-based bioprinting enables precise drop-on-demand cell deposition within three-dimensional (3D) tissue constructs and facilitates critical cell–cell and cell–matrix interactions, it faces challenges such as poor cell homogeneity and low cell viability. To date, there is a lack of comprehensive review papers addressing the optimization of cell deposition in inkjet-based bioprinting. This review aims to fill that gap by providing an overview of various critical aspects in bioprinting, ranging from bio-ink properties to the impact of printed droplets. The bio-ink section begins by exploring how cells influence the physical properties of bio-inks and emphasizes the significance of achieving cell homogeneity within bio-inks to ensure consistent and reliable printing. The discussion then delves into inkjet-based printing chambers (thermal and piezoelectric), the effect of shear stress on printed cells, droplet formation dynamics, the influence of polymer-based and cell-laden droplets on the underlying substrate surface, and the dynamics of droplet impact. Beyond droplet formation and impact, the review highlights the importance of biophysical and biological cues within 3D hydrogel matrices for cell proliferation and differentiation. Finally, the paper highlights current and potential applications, with a specific focus on skin and lung tissue engineering using inkjet-based bioprinting techniques, and provides insights into the emerging role of machine learning in optimizing the cell deposition process for inkjet-based bioprinting.
{"title":"Optimizing cell deposition for inkjet-based bioprinting","authors":"Wei Long Ng, V. Shkolnikov","doi":"10.36922/ijb.2135","DOIUrl":"https://doi.org/10.36922/ijb.2135","url":null,"abstract":"Although inkjet-based bioprinting enables precise drop-on-demand cell deposition within three-dimensional (3D) tissue constructs and facilitates critical cell–cell and cell–matrix interactions, it faces challenges such as poor cell homogeneity and low cell viability. To date, there is a lack of comprehensive review papers addressing the optimization of cell deposition in inkjet-based bioprinting. This review aims to fill that gap by providing an overview of various critical aspects in bioprinting, ranging from bio-ink properties to the impact of printed droplets. The bio-ink section begins by exploring how cells influence the physical properties of bio-inks and emphasizes the significance of achieving cell homogeneity within bio-inks to ensure consistent and reliable printing. The discussion then delves into inkjet-based printing chambers (thermal and piezoelectric), the effect of shear stress on printed cells, droplet formation dynamics, the influence of polymer-based and cell-laden droplets on the underlying substrate surface, and the dynamics of droplet impact. Beyond droplet formation and impact, the review highlights the importance of biophysical and biological cues within 3D hydrogel matrices for cell proliferation and differentiation. Finally, the paper highlights current and potential applications, with a specific focus on skin and lung tissue engineering using inkjet-based bioprinting techniques, and provides insights into the emerging role of machine learning in optimizing the cell deposition process for inkjet-based bioprinting.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139865158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nazi Zhou, Shunyao Zhu, Xinlin Wei, Xueyuan Liao, Yu Wang, Yue Xu, Liyun Bai, Haoyuan Wan, Li Liu, Jiumeng Zhang, Ling Zeng, Jie Tao, Rui Liu
Infections to dental pulp commonly result in pulpitis and pulp necrosis, and surgical removal of the infected tissues is the only therapeutic approach. Dental pulp injury remains a challenging medical issue due to the limited regenerative capability of dental pulp. In this work, a dental pulp guidance construct (DPGC) with the instructive niche was bioprinted to mimic native teeth for dentin and neovascular-like structure reconstruction. GelMA-Dextran aqueous emulsion was used as an ink for in situ printing of porous DPGC to induce predominant nuclear localization of Yes-associated protein (YAP) in the encapsulated dental pulp stem cells (DPSCs) and enhance their stemness properties. Furthermore, the DPSCs encapsulated in DPGC with microporous structures exhibited enhanced viability, migration, and spreading. Meanwhile, we found that DPGC could promote capillary tube formation and induce neurogenesis. In a mouse subcutaneous implant model, the DPGC consisted of porous structures, such as odontoblasts and newly formed vascular structures, that mimic dental pulp characteristics. This study demonstrated a new strategy to design DPGC with instructive niche for dental pulp regeneration, presenting a potential treatment alternative to root canal therapy.
{"title":"3D-bioprinted hydrogels with instructive niches for dental pulp regeneration","authors":"Nazi Zhou, Shunyao Zhu, Xinlin Wei, Xueyuan Liao, Yu Wang, Yue Xu, Liyun Bai, Haoyuan Wan, Li Liu, Jiumeng Zhang, Ling Zeng, Jie Tao, Rui Liu","doi":"10.36922/ijb.1790","DOIUrl":"https://doi.org/10.36922/ijb.1790","url":null,"abstract":"Infections to dental pulp commonly result in pulpitis and pulp necrosis, and surgical removal of the infected tissues is the only therapeutic approach. Dental pulp injury remains a challenging medical issue due to the limited regenerative capability of dental pulp. In this work, a dental pulp guidance construct (DPGC) with the instructive niche was bioprinted to mimic native teeth for dentin and neovascular-like structure reconstruction. GelMA-Dextran aqueous emulsion was used as an ink for in situ printing of porous DPGC to induce predominant nuclear localization of Yes-associated protein (YAP) in the encapsulated dental pulp stem cells (DPSCs) and enhance their stemness properties. Furthermore, the DPSCs encapsulated in DPGC with microporous structures exhibited enhanced viability, migration, and spreading. Meanwhile, we found that DPGC could promote capillary tube formation and induce neurogenesis. In a mouse subcutaneous implant model, the DPGC consisted of porous structures, such as odontoblasts and newly formed vascular structures, that mimic dental pulp characteristics. This study demonstrated a new strategy to design DPGC with instructive niche for dental pulp regeneration, presenting a potential treatment alternative to root canal therapy.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139804284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bioprinting is a novel technique with a wide range of potential uses, including the fabrication of functioning tissue constructs for use in the biomedical sectors. It is a revolutionary method for high-throughput manufacturing that automates fine control over manufactured structures. Bioink refers to the solution of biomaterials usually encapsulating cells used in the bioprinting process; this bioink often encapsulates the appropriate cell types. In order to create the ultimate architecture, this bioink should solidify during or shortly after bioprinting. Bioinks can be developed from either all-natural or all-synthetic biomaterials, or a blend of the two. Cell aggregation can occasionally be used as a bioink without addition of any biomaterials, in bioprinting process. To bioprint functional tissues and organs, an optimal bioink should possess mechanical, rheological, and biological characteristics mimicking those of the target tissues. For attaining physicomechanical properties, anisotropic fillers are commonly added in bioink formulations. In this review, we provide an in-depth discussion of various anisotropic fillers used in bioprinting and their fabrication techniques, and outline their multifunctional applicability in biomedical and environmental areas. Given the steady growth of bioprinting market, we also present the global scenario of the bioprinting market and their techno-commercial orientations.
{"title":"3D bioprinting of anisotropic filler-reinforced polymer nanocomposites: Synthesis, assembly, and multifunctional applications","authors":"Yuan Wu, Sayan Ganguly, X. Tang","doi":"10.36922/ijb.1637","DOIUrl":"https://doi.org/10.36922/ijb.1637","url":null,"abstract":"Bioprinting is a novel technique with a wide range of potential uses, including the fabrication of functioning tissue constructs for use in the biomedical sectors. It is a revolutionary method for high-throughput manufacturing that automates fine control over manufactured structures. Bioink refers to the solution of biomaterials usually encapsulating cells used in the bioprinting process; this bioink often encapsulates the appropriate cell types. In order to create the ultimate architecture, this bioink should solidify during or shortly after bioprinting. Bioinks can be developed from either all-natural or all-synthetic biomaterials, or a blend of the two. Cell aggregation can occasionally be used as a bioink without addition of any biomaterials, in bioprinting process. To bioprint functional tissues and organs, an optimal bioink should possess mechanical, rheological, and biological characteristics mimicking those of the target tissues. For attaining physicomechanical properties, anisotropic fillers are commonly added in bioink formulations. In this review, we provide an in-depth discussion of various anisotropic fillers used in bioprinting and their fabrication techniques, and outline their multifunctional applicability in biomedical and environmental areas. Given the steady growth of bioprinting market, we also present the global scenario of the bioprinting market and their techno-commercial orientations.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139804991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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