Liang Chen, Guowei Huang, Ming-Han Yu, Yang Liu, Tao Cheng, Aiguo Li, Wen Wang, Shengnan Qin
The structure and composition of articular cartilage is complex, and its self-healing ability is limited, and thus, it is difficult to achieve ideal healing once the articular cartilage is damaged. Recently, three-dimensional (3D) printing technology has provided a new possibility for the repair of articular cartilage. Engineered cartilage tissues can be fabricated by superimposing customized inks, considering different geometric structures and components of tissues. 3D printing can be effectively used to manufacture high-precision structures with complex geometry, solving the shortcomings of traditional scaffold fabrication techniques. Gelatin methacrylate (GelMA) is modified gelatin and is currently a widely used 3D printing ink due to its photocrosslinking properties. With good biocompatibility and tunable physical properties, it can provide a good scaffold platform for cell proliferation and growth factor release. Given that the role of 3D printing technology in cartilage repair has been widely reported, this article reviews the research progress of 3D-printed GelMA-based biomaterials in articular cartilage tissue engineering. We focus primarily on how 3D printing technology addresses the existing challenges inherent to the field of articular cartilage tissue engineering. We accentuate the modifications implemented in GelMA-based 3D printing scaffolds to optimize articular cartilage regeneration. Additionally, we provide a comprehensive summary of the utilization of GelMA-based biomaterials incorporating various cells, growth factors, or other tissue components and highlight how these adaptations, in conjunction with the benefits of 3D printing technology, facilitate improvements the articular cartilage repair.
{"title":"Recent progress on 3D-printed gelatin methacrylate-based biomaterials for articular cartilage repair ","authors":"Liang Chen, Guowei Huang, Ming-Han Yu, Yang Liu, Tao Cheng, Aiguo Li, Wen Wang, Shengnan Qin","doi":"10.36922/ijb.0116","DOIUrl":"https://doi.org/10.36922/ijb.0116","url":null,"abstract":"The structure and composition of articular cartilage is complex, and its self-healing ability is limited, and thus, it is difficult to achieve ideal healing once the articular cartilage is damaged. Recently, three-dimensional (3D) printing technology has provided a new possibility for the repair of articular cartilage. Engineered cartilage tissues can be fabricated by superimposing customized inks, considering different geometric structures and components of tissues. 3D printing can be effectively used to manufacture high-precision structures with complex geometry, solving the shortcomings of traditional scaffold fabrication techniques. Gelatin methacrylate (GelMA) is modified gelatin and is currently a widely used 3D printing ink due to its photocrosslinking properties. With good biocompatibility and tunable physical properties, it can provide a good scaffold platform for cell proliferation and growth factor release. Given that the role of 3D printing technology in cartilage repair has been widely reported, this article reviews the research progress of 3D-printed GelMA-based biomaterials in articular cartilage tissue engineering. We focus primarily on how 3D printing technology addresses the existing challenges inherent to the field of articular cartilage tissue engineering. We accentuate the modifications implemented in GelMA-based 3D printing scaffolds to optimize articular cartilage regeneration. Additionally, we provide a comprehensive summary of the utilization of GelMA-based biomaterials incorporating various cells, growth factors, or other tissue components and highlight how these adaptations, in conjunction with the benefits of 3D printing technology, facilitate improvements the articular cartilage repair.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"1 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80578932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fan Xu, Shunli Rui, Cheng Yang, Xiaoyan Jiang, Wei Wu, Xianlun Tang, David G Armstrong, Yu Ma, Wu Deng
Most conventional therapies have limitations in the repair of complex wounds caused by chronic inflammation in patients with diabetic foot ulcers (DFUs). In response to the demand for more biotechnology strategies, bioprinting has been explored in the regeneration field in recent years. However, challenges remain regarding the structure of complex models and the selection of proper biomaterials. The purpose of this review is to introduce the current applications of bioprinting technology in chronic diabetic foot wound healing. First, the most common application of bioprinting in producing skin equivalents to promote wound healing is introduced; second, functional improvements in the treatment of chronic and difficult-to-heal DFU wounds facilitated by bioprinting applications are discussed; and last but not least, bioprinting applications in addressing unique diabetic foot disease characteristics are summarized. Furthermore, the present work summarizes material selection and correlations between three-dimensional (3D) bioprinting and a variety of biomimetic strategies for accelerating wound healing. Novel, biotechnological tools such as organoids for developing new biomaterials for bioprinting in the future are also discussed.
{"title":"Bioprinting technology for the management of diabetic foot disease: Emerging applications, challenges, and prospects","authors":"Fan Xu, Shunli Rui, Cheng Yang, Xiaoyan Jiang, Wei Wu, Xianlun Tang, David G Armstrong, Yu Ma, Wu Deng","doi":"10.36922/ijb.0142","DOIUrl":"https://doi.org/10.36922/ijb.0142","url":null,"abstract":"Most conventional therapies have limitations in the repair of complex wounds caused by chronic inflammation in patients with diabetic foot ulcers (DFUs). In response to the demand for more biotechnology strategies, bioprinting has been explored in the regeneration field in recent years. However, challenges remain regarding the structure of complex models and the selection of proper biomaterials. The purpose of this review is to introduce the current applications of bioprinting technology in chronic diabetic foot wound healing. First, the most common application of bioprinting in producing skin equivalents to promote wound healing is introduced; second, functional improvements in the treatment of chronic and difficult-to-heal DFU wounds facilitated by bioprinting applications are discussed; and last but not least, bioprinting applications in addressing unique diabetic foot disease characteristics are summarized. Furthermore, the present work summarizes material selection and correlations between three-dimensional (3D) bioprinting and a variety of biomimetic strategies for accelerating wound healing. Novel, biotechnological tools such as organoids for developing new biomaterials for bioprinting in the future are also discussed.\u0000 ","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"23 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82175129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ruiquan Liu, Litao Jia, Jianguo Chen, Y. Long, Jinshi Zeng, Siyu Liu, Bo Pan, Xia Liu, Haiyue Jiang
Three-dimensional (3D) bioprinting has brought new promising strategies for the regeneration of cartilage with specific shapes. In cartilage bioprinting, chondrocyte-laden hydrogels are the most commonly used bioinks. However, the dispersion of cells and the dense texture of the hydrogel in the conventional bioink may limit cell–cell/ cell–extracellular matrix (ECM) interactions, counting against cartilage regeneration and maturation. To address this issue, in this study, we developed a functional bioink for cartilage bioprinting based on chondrocyte spheroids (CSs) and microporous hydrogels, in which CSs as multicellular aggregates can provide extensive cell– cell/cell–ECM interactions to mimic the natural cartilage microenvironment, and microporous hydrogels can provide space and channel for the growth and fusion of the CSs. Firstly, we used a non-adhesive microporous system to produce homogeneous self-assembled CSs in high-throughput and evaluated the influence of different CSs preparation parameters (cell number and culture time) on CSs, which aids in the preparation of bioink suitable for cartilage bioprinting. Then, polyethylene oxide (PEO) was introduced into gelatin methacrylate (GelMA) to prepare microporous hydrogel. Finally, the CS-laden microporous hydrogels were printed, and the constructs were implanted into nude mice. The results showed that the CSs with 500 cells cultured for 1 day exhibited better proliferation and growth ability in microporous hydrogels compared to those with more cells and cultured for longer time. In addition, the results also demonstrated that the CS-laden bioink can be successfully printed into predefined lattice-shape constructs with little cell damage and regenerated cartilage tissue in vivo with a structure similar to natural cartilage characterized by typical lacunae structure and abundant cartilage-specific ECM deposition. In summary, our study verified the feasibility and advantages of using CSs as building blocks in cartilage bioprinting, which provides novel strategies for the fabrication and regeneration of patient-specific shaped cartilage.
{"title":"Chondrocyte spheroid-laden microporous hydrogel-based 3D bioprinting for cartilage regeneration","authors":"Ruiquan Liu, Litao Jia, Jianguo Chen, Y. Long, Jinshi Zeng, Siyu Liu, Bo Pan, Xia Liu, Haiyue Jiang","doi":"10.36922/ijb.0161","DOIUrl":"https://doi.org/10.36922/ijb.0161","url":null,"abstract":"Three-dimensional (3D) bioprinting has brought new promising strategies for the regeneration of cartilage with specific shapes. In cartilage bioprinting, chondrocyte-laden hydrogels are the most commonly used bioinks. However, the dispersion of cells and the dense texture of the hydrogel in the conventional bioink may limit cell–cell/ cell–extracellular matrix (ECM) interactions, counting against cartilage regeneration and maturation. To address this issue, in this study, we developed a functional bioink for cartilage bioprinting based on chondrocyte spheroids (CSs) and microporous hydrogels, in which CSs as multicellular aggregates can provide extensive cell– cell/cell–ECM interactions to mimic the natural cartilage microenvironment, and microporous hydrogels can provide space and channel for the growth and fusion of the CSs. Firstly, we used a non-adhesive microporous system to produce homogeneous self-assembled CSs in high-throughput and evaluated the influence of different CSs preparation parameters (cell number and culture time) on CSs, which aids in the preparation of bioink suitable for cartilage bioprinting. Then, polyethylene oxide (PEO) was introduced into gelatin methacrylate (GelMA) to prepare microporous hydrogel. Finally, the CS-laden microporous hydrogels were printed, and the constructs were implanted into nude mice. The results showed that the CSs with 500 cells cultured for 1 day exhibited better proliferation and growth ability in microporous hydrogels compared to those with more cells and cultured for longer time. In addition, the results also demonstrated that the CS-laden bioink can be successfully printed into predefined lattice-shape constructs with little cell damage and regenerated cartilage tissue in vivo with a structure similar to natural cartilage characterized by typical lacunae structure and abundant cartilage-specific ECM deposition. In summary, our study verified the feasibility and advantages of using CSs as building blocks in cartilage bioprinting, which provides novel strategies for the fabrication and regeneration of patient-specific shaped cartilage.\u0000 ","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"23 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78358794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Ianchiș, Maria Minodora Marin, Rebeca Leu Alexa, Ioana Catalina Gifu, E. Alexandrescu, G. Pîrcălăbioru, George Mihail Vlasceanu, George Mihail Teodorescu, A. Serafim, S. Preda, Cristina Lavinia Nistor, C. Petcu
The main objective of the present work was to produce three-dimensional (3D)- printable nanocomposite hydrogels based on two kinds of marine-sourced polysaccharides doped with nanoclay with potential biomedical application. First part of the research study investigated the preparation of the polysaccharide bicomponent hydrogel formulations followed by the selection of the optimal ratio of polysaccharides concentrations which ensured proper morphostructural stability of the 3D-printed constructs. Second step aimed to generate 3D scaffolds with high printing fidelity by modulating the nanoclay amount doped within the previously selected biopolymer ink. In compliance with the additive manufacturing experiments, the alginate–salecan hydrogels enriched with the highest nanofiller concentrations demonstrated the highest suitability for 3D printing process. The morphological and structural studies confirmed the ability of the nanocomposite formulations to efficiently produce porous 3D-printed constructs with improved fidelity. The morphostructural findings underlined the implication of choosing the appropriate ratio between components, as they have a considerable impact on the functionality of printing formulations and subsequent 3D-printed structures. Hence, from the obtained results, these novel hydrogel nanocomposites inks are considered valuable biomaterials with suitable features for applications in the additive manufacturing of 3D structures with precise shape for customized regenerative therapy.
{"title":"Nanoclay-reinforced alginate/salecan composite inks for 3D printing applications","authors":"R. Ianchiș, Maria Minodora Marin, Rebeca Leu Alexa, Ioana Catalina Gifu, E. Alexandrescu, G. Pîrcălăbioru, George Mihail Vlasceanu, George Mihail Teodorescu, A. Serafim, S. Preda, Cristina Lavinia Nistor, C. Petcu","doi":"10.36922/ijb.0967","DOIUrl":"https://doi.org/10.36922/ijb.0967","url":null,"abstract":"The main objective of the present work was to produce three-dimensional (3D)- printable nanocomposite hydrogels based on two kinds of marine-sourced polysaccharides doped with nanoclay with potential biomedical application. First part of the research study investigated the preparation of the polysaccharide bicomponent hydrogel formulations followed by the selection of the optimal ratio of polysaccharides concentrations which ensured proper morphostructural stability of the 3D-printed constructs. Second step aimed to generate 3D scaffolds with high printing fidelity by modulating the nanoclay amount doped within the previously selected biopolymer ink. In compliance with the additive manufacturing experiments, the alginate–salecan hydrogels enriched with the highest nanofiller concentrations demonstrated the highest suitability for 3D printing process. The morphological and structural studies confirmed the ability of the nanocomposite formulations to efficiently produce porous 3D-printed constructs with improved fidelity. The morphostructural findings underlined the implication of choosing the appropriate ratio between components, as they have a considerable impact on the functionality of printing formulations and subsequent 3D-printed structures. Hence, from the obtained results, these novel hydrogel nanocomposites inks are considered valuable biomaterials with suitable features for applications in the additive manufacturing of 3D structures with precise shape for customized regenerative therapy.\u0000 ","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79926442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haoyu Li, Huixing Zhou, Chongwen Xu, Yen Wei, Xiuying Tang
The biofabrication of multi-cellular tissues or organoids (MTOs) has been challenging in regenerative medicine for decades. Currently, two primary technological approaches are being explored: scaffold-based strategies utilizing three-dimensional (3D) bioprinting techniques and scaffold-free strategies employing bioassembly techniques. 3D bioprinting techniques include jetting-based, extrusion-based, and vat photopolymerization-based methods, and bioassembly techniques include Kenzan, fluid-based manipulation and microfluid, bioprinting-assisted tissue emergence, and aspiration-assisted technology methods. Scaffold-based strategies primarily concentrate on the construction of scaffold structures to provide an extracellular environment, while scaffold-free strategies primarily emphasize the assembly methods of building blocks. Different biofabrication technologies have their advantages and limitations. This review provides an overview of the mechanisms, advantages, and limitations of scaffold-based and scaffold-free strategies in tissue engineering. It also compares the strengths and weaknesses of these two strategies, along with their respective suitability under different conditions. Moreover, the significant challenges in the future development of convergence strategies, specifically the integration of scaffold-based and scaffold-free approaches, are examined in an objective manner. This review concludes that integrating scaffold-based and scaffold-free strategies could overcome the problems in the biofabrication of MTOs. A novel fabrication method, the BioMicroMesh method, is proposed based on the convergence strategy. Concurrently, the development of a desktop-scale integrated intelligent biofabrication device, the BioMicroMesh system, is underway. This system is tailored to the BioMicroMesh method and incorporates cell aggregate spheroids preparation, 3D bioprinting, bioassembly, and multi-organoid co-culture functions, providing an objective perspective on its capabilities.
{"title":"3D bioprinting and scaffold-free strategies for fabrication of multi-cellular tissues or organoids","authors":"Haoyu Li, Huixing Zhou, Chongwen Xu, Yen Wei, Xiuying Tang","doi":"10.36922/ijb.0135","DOIUrl":"https://doi.org/10.36922/ijb.0135","url":null,"abstract":"The biofabrication of multi-cellular tissues or organoids (MTOs) has been challenging in regenerative medicine for decades. Currently, two primary technological approaches are being explored: scaffold-based strategies utilizing three-dimensional (3D) bioprinting techniques and scaffold-free strategies employing bioassembly techniques. 3D bioprinting techniques include jetting-based, extrusion-based, and vat photopolymerization-based methods, and bioassembly techniques include Kenzan, fluid-based manipulation and microfluid, bioprinting-assisted tissue emergence, and aspiration-assisted technology methods. Scaffold-based strategies primarily concentrate on the construction of scaffold structures to provide an extracellular environment, while scaffold-free strategies primarily emphasize the assembly methods of building blocks. Different biofabrication technologies have their advantages and limitations. This review provides an overview of the mechanisms, advantages, and limitations of scaffold-based and scaffold-free strategies in tissue engineering. It also compares the strengths and weaknesses of these two strategies, along with their respective suitability under different conditions. Moreover, the significant challenges in the future development of convergence strategies, specifically the integration of scaffold-based and scaffold-free approaches, are examined in an objective manner. This review concludes that integrating scaffold-based and scaffold-free strategies could overcome the problems in the biofabrication of MTOs. A novel fabrication method, the BioMicroMesh method, is proposed based on the convergence strategy. Concurrently, the development of a desktop-scale integrated intelligent biofabrication device, the BioMicroMesh system, is underway. This system is tailored to the BioMicroMesh method and incorporates cell aggregate spheroids preparation, 3D bioprinting, bioassembly, and multi-organoid co-culture functions, providing an objective perspective on its capabilities.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"542 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77178097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yangjing Lin, P. He, Guangyu Yang, Fuyou Wang, L. Jia, Huaquan Fan, Liu Yang, Huiping Tang, Xiaojun Duan
In foot and ankle surgery, internal fixation was crucial to maintain the stability of bony structure, and bone grafting material is commonly used to treat bone defects. With rapid development of three-dimensional (3D) printing technology, new advances were made in these two aspects. In this study, digital image correlation method (DICM) data of the patient’s ankle via computed tomography (CT) examination were obtained and imported into a series of software. The engineer cooperated with the surgeon to design the customized implants. Ti-6Al-4V spherical metal powder was chosen as raw material and fused together by selective electron beam melting (SEBM), a type of 3D printing technology, to prepare the implant. The implants were sterilized with ethylene oxide. The customized 3D-printed implants were successfully utilized in tibiotalocalcaneal (TTC) arthrodesis to maintain the bony structures at the functional position. In another case, the 3D-printed fusion cage was applied in subtalar arthrodesis to treat bone defects. In these clinical cases, 3D-printed customized titanium implants helped improve the surgical operation flow, and no obvious tissue reaction was observed. The successful implementation suggested that the application of 3D printing technology to prepare customized titanium implants would play an important role in future foot and ankle surgery.
在足踝外科手术中，内固定是保持骨结构稳定的关键，而骨移植材料则常用于治疗骨缺损。随着三维打印技术的快速发展，这两方面都取得了新的进展。本研究通过计算机断层扫描（CT）获取了患者踝关节的数字图像关联法（DICM）数据，并将其导入一系列软件。工程师与外科医生合作设计了定制植入物。选用 Ti-6Al-4V 球形金属粉末作为原材料，并通过选择性电子束熔化（SEMM）（一种三维打印技术）将其融合在一起，制备植入体。植入体用环氧乙烷灭菌。定制的三维打印植入物被成功用于胫骨-腓骨-踝骨（TTC）关节成形术，将骨性结构保持在功能位置。在另一个病例中，三维打印融合笼被应用于距骨下关节成形术，以治疗骨缺损。在这些临床病例中，3D打印定制钛植入物有助于改善手术操作流程，且未观察到明显的组织反应。这些案例的成功实施表明，应用 3D 打印技术制备定制钛植入物将在未来的足踝外科手术中发挥重要作用。
{"title":"Efficacy of 3D-printed customized titanium implants and its clinical validation in foot and ankle surgery","authors":"Yangjing Lin, P. He, Guangyu Yang, Fuyou Wang, L. Jia, Huaquan Fan, Liu Yang, Huiping Tang, Xiaojun Duan","doi":"10.36922/ijb.0125","DOIUrl":"https://doi.org/10.36922/ijb.0125","url":null,"abstract":"In foot and ankle surgery, internal fixation was crucial to maintain the stability of bony structure, and bone grafting material is commonly used to treat bone defects. With rapid development of three-dimensional (3D) printing technology, new advances were made in these two aspects. In this study, digital image correlation method (DICM) data of the patient’s ankle via computed tomography (CT) examination were obtained and imported into a series of software. The engineer cooperated with the surgeon to design the customized implants. Ti-6Al-4V spherical metal powder was chosen as raw material and fused together by selective electron beam melting (SEBM), a type of 3D printing technology, to prepare the implant. The implants were sterilized with ethylene oxide. The customized 3D-printed implants were successfully utilized in tibiotalocalcaneal (TTC) arthrodesis to maintain the bony structures at the functional position. In another case, the 3D-printed fusion cage was applied in subtalar arthrodesis to treat bone defects. In these clinical cases, 3D-printed customized titanium implants helped improve the surgical operation flow, and no obvious tissue reaction was observed. The successful implementation suggested that the application of 3D printing technology to prepare customized titanium implants would play an important role in future foot and ankle surgery.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"79 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139355761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Angelica A. Martinez-De-Anda, M. Rodríguez-Salvador, Pedro F. Castillo-Valdez
Currently, there is an increasing demand in the medical field for low-cost, high-quality products as well as personalized solutions, and different manufacturing methods are being investigated to provide innovative solutions. Four-dimensional (4D) printing is a promising technology that could overcome the challenges associated with the applications of three-dimensional printing in regenerative medicine and biomedical devices. A revolution is expected in this regard, and it is essential to keep abreast of the latest developments. Under this context, the purpose of this paper is to reveal scientific and technological advances of 4D printing in the medical field using a competitive technology intelligence (CTI) methodology. To this end, publications were analyzed from the Scopus database between January 1, 2017, and May 9, 2023. The main trends were identified for both design factors and applications. In the first case, the following were considered: 4D printing methods, external stimuli, materials, mathematical models, and interaction mechanisms. In contrast, in the second case, the applications of 4D printing involved were considered: drug delivery systems, stents, and scaffolds. The obtained design factors results included improvements in mechanical properties of hydrogels by adding magnetic nanoparticles, biopolyurethane, and other materials, the development of cell-friendly bioprinting methods to print cellular structures, and the use of theoretical-experimental approaches to predict shape deformation of structures. While for applications, results included advances in the development of expandable drug delivery systems, fabrication of stents for the treatment of vascular and tracheal stenosis, and the design of scaffolds to treat cartilage defects and bone regeneration. This study provides insights to researchers, academics, and companies involved in research and development as well as innovation that are looking for new solutions to improve health by incorporating breakthrough technologies such as 4D printing.
{"title":"The attractiveness of 4D printing in the medical field: Revealing scientific and technological advances in design factors and applications ","authors":"Angelica A. Martinez-De-Anda, M. Rodríguez-Salvador, Pedro F. Castillo-Valdez","doi":"10.36922/ijb.1112","DOIUrl":"https://doi.org/10.36922/ijb.1112","url":null,"abstract":"Currently, there is an increasing demand in the medical field for low-cost, high-quality products as well as personalized solutions, and different manufacturing methods are being investigated to provide innovative solutions. Four-dimensional (4D) printing is a promising technology that could overcome the challenges associated with the applications of three-dimensional printing in regenerative medicine and biomedical devices. A revolution is expected in this regard, and it is essential to keep abreast of the latest developments. Under this context, the purpose of this paper is to reveal scientific and technological advances of 4D printing in the medical field using a competitive technology intelligence (CTI) methodology. To this end, publications were analyzed from the Scopus database between January 1, 2017, and May 9, 2023. The main trends were identified for both design factors and applications. In the first case, the following were considered: 4D printing methods, external stimuli, materials, mathematical models, and interaction mechanisms. In contrast, in the second case, the applications of 4D printing involved were considered: drug delivery systems, stents, and scaffolds. The obtained design factors results included improvements in mechanical properties of hydrogels by adding magnetic nanoparticles, biopolyurethane, and other materials, the development of cell-friendly bioprinting methods to print cellular structures, and the use of theoretical-experimental approaches to predict shape deformation of structures. While for applications, results included advances in the development of expandable drug delivery systems, fabrication of stents for the treatment of vascular and tracheal stenosis, and the design of scaffolds to treat cartilage defects and bone regeneration. This study provides insights to researchers, academics, and companies involved in research and development as well as innovation that are looking for new solutions to improve health by incorporating breakthrough technologies such as 4D printing.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"70 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90682035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study established and evaluated the feasibility of a three-dimensional (3D)- printed titanium anatomical surface with adjustable thin bone plate assembly (AATBP) for patella fracture fixation. The AATBP was 1.6 mm in thickness and divided into a proximal plate (PP) with locking screw holes and a distal plate (DP) (0.4 mm in thickness) with compressive screw holes for assembly using a ratchet mechanism to adjust the total fixation height according to the patella size. Two pairs of hooks were designed on the proximal/distal edges to allow passage through the tendon to grip the fractured fragments. 3D printing combined with Computer Numerical Control (CNC) drilling was performed to manufacture the AATBP. Four-point bending and surface roughness tests were performed to evaluate the AATBP mechanical behavior. A cyclic (300 times) load test with 15-kg weights was adopted to compare the biomechanical stability between the AATBP and conventional tension band wiring (TBW) fixations. A parallel finite element (FE) analysis was achieved to understand the fracture gap and bone stress in the two different fixations on a transverse patella fracture. The result showed that the maximum AATBP manufacturing error was 3.75%. The average fracture gaps on the medial/lateral sides after cyclic loads were 2.38 ± 0.57 mm/2.30 ± 0.30 mm for TBW and 0.03 ± 0.01 mm/0.06 ± 0.03 mm for AATBP fixations. The same trend occurred in the FE simulation. This study confirmed that a complicated thin bone plate, including the anatomical surface, hooks, and ratchet with size-adjustable characteristics, can be fabricated using metal 3D printing with acceptable manufacturing error and reasonable anatomical surface/ thin bone plate assembly fitness. Biomechanical cyclic tests and FE simulation showed that the AATBP fixation is superior to the conventional TBW for patella transverse fractures.
{"title":"Biomechanical evaluation of an anatomical bone plate assembly for thin patella fracture fixation fabricated by titanium alloy 3D printing ","authors":"Chi-Yang Liao, Shao-Fu Huang, Wei-Che Tsai, Yukun Zeng, Chia-Hsuan Li, Chun-Li Lin","doi":"10.36922/ijb.0117","DOIUrl":"https://doi.org/10.36922/ijb.0117","url":null,"abstract":"This study established and evaluated the feasibility of a three-dimensional (3D)- printed titanium anatomical surface with adjustable thin bone plate assembly (AATBP) for patella fracture fixation. The AATBP was 1.6 mm in thickness and divided into a proximal plate (PP) with locking screw holes and a distal plate (DP) (0.4 mm in thickness) with compressive screw holes for assembly using a ratchet mechanism to adjust the total fixation height according to the patella size. Two pairs of hooks were designed on the proximal/distal edges to allow passage through the tendon to grip the fractured fragments. 3D printing combined with Computer Numerical Control (CNC) drilling was performed to manufacture the AATBP. Four-point bending and surface roughness tests were performed to evaluate the AATBP mechanical behavior. A cyclic (300 times) load test with 15-kg weights was adopted to compare the biomechanical stability between the AATBP and conventional tension band wiring (TBW) fixations. A parallel finite element (FE) analysis was achieved to understand the fracture gap and bone stress in the two different fixations on a transverse patella fracture. The result showed that the maximum AATBP manufacturing error was 3.75%. The average fracture gaps on the medial/lateral sides after cyclic loads were 2.38 ± 0.57 mm/2.30 ± 0.30 mm for TBW and 0.03 ± 0.01 mm/0.06 ± 0.03 mm for AATBP fixations. The same trend occurred in the FE simulation. This study confirmed that a complicated thin bone plate, including the anatomical surface, hooks, and ratchet with size-adjustable characteristics, can be fabricated using metal 3D printing with acceptable manufacturing error and reasonable anatomical surface/ thin bone plate assembly fitness. Biomechanical cyclic tests and FE simulation showed that the AATBP fixation is superior to the conventional TBW for patella transverse fractures.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"10 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85124880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gastrointestinal (GI) system comprises a great number of organs and tissues of various functions, both hollow and solid. However, it is still a less well-developed area for three-dimensional (3D) printing (3DP) applications compared to orthopedics. Clinical applications of 3DP in the GI system are presently restricted to preoperative planning, surgical guidance, and education for students, residents, and patients, either for laparoscopy or endoscopy. Several surgery-related accessories have been designed to facilitate surgical procedures. The results are promising but not adequately proven due to a lack of reasonable study design and proper comparisons. Other important requirements for GI systems in clinical scenarios are structural reconstruction, replacement, defect repair, drug screening, and delivery. Many 3D-printed decellularized, cell-seeded, or even bioprinted scaffolds have been studied; however, most studies were conducted on small animal or in vitro models. Although encouraging results have been obtained, there is still a long way to go before products compatible with humans in size, histology, and functions can be printed. The key points to achieving this goal are the printing material, cell type and source, and printing technology. The ultimate goal is to print tissue and organ substitutes with physiological functions for clinical purposes in both time- and cost-effective ways.
{"title":"Advances of 3D printing in gastroenterology and where it might be going","authors":"Yu-hang Zhang, Liuxiang Chen, Bing Hu","doi":"10.36922/ijb.0149","DOIUrl":"https://doi.org/10.36922/ijb.0149","url":null,"abstract":"Gastrointestinal (GI) system comprises a great number of organs and tissues of various functions, both hollow and solid. However, it is still a less well-developed area for three-dimensional (3D) printing (3DP) applications compared to orthopedics. Clinical applications of 3DP in the GI system are presently restricted to preoperative planning, surgical guidance, and education for students, residents, and patients, either for laparoscopy or endoscopy. Several surgery-related accessories have been designed to facilitate surgical procedures. The results are promising but not adequately proven due to a lack of reasonable study design and proper comparisons. Other important requirements for GI systems in clinical scenarios are structural reconstruction, replacement, defect repair, drug screening, and delivery. Many 3D-printed decellularized, cell-seeded, or even bioprinted scaffolds have been studied; however, most studies were conducted on small animal or in vitro models. Although encouraging results have been obtained, there is still a long way to go before products compatible with humans in size, histology, and functions can be printed. The key points to achieving this goal are the printing material, cell type and source, and printing technology. The ultimate goal is to print tissue and organ substitutes with physiological functions for clinical purposes in both time- and cost-effective ways.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"50 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75143280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pengli Wang, Tao Wang, Yong Xu, Nankai Song, Xue Zhang
Despite the notable advances in tissue-engineered tracheal cartilage (TETC), there remain several challenges that need to be addressed, such as uneven cell distribution for cartilage formation, customized C-shaped tracheal morphology, local inflammatory reactions, and infections. To overcome these challenges, this study proposed the addition of icariin (ICA) and chitosan (CS) into a gelatin methacryloyl (GelMA) hydrogel to develop a new ICA/CS/GelMA hydrogel with anti-inflammatory and anti-bacterial properties, and three-dimensional (3D)-bioprinting feasibility. The aim of this study was to construct a TETC, a customized C-shaped cartilage structure, with uniform chondrocyte distribution as well as anti-inflammatory and anti-bacterial functions. Our results confirmed that ICA/CS/GelMA hydrogel provides desirable rheological properties, suitable printability, favorable biocompatibility, and simulated microenvironments for chondrogenesis. Moreover, the addition of ICA stimulated chondrocyte proliferation, extracellular matrix synthesis, and anti-inflammatory ability, while the encapsulation of CS enhanced the hydrogels’ anti-bacterial ability. All these led to the formation of an enhanced TETC after submuscular implantation and an elevated survival rate of experimental rabbits after orthotopic tracheal transplantation. This study provides a reliable cell-laden hydrogel with anti-inflammatory and anti-bacterial activities, suitable printability, and significant advancements in in vivo cartilage regeneration and in situ tracheal cartilage restoration.
{"title":"3D-bioprinted cell-laden hydrogel with anti-inflammatory and anti-bacterial activities for tracheal cartilage regeneration and restoration","authors":"Pengli Wang, Tao Wang, Yong Xu, Nankai Song, Xue Zhang","doi":"10.36922/ijb.0146","DOIUrl":"https://doi.org/10.36922/ijb.0146","url":null,"abstract":"Despite the notable advances in tissue-engineered tracheal cartilage (TETC), there remain several challenges that need to be addressed, such as uneven cell distribution for cartilage formation, customized C-shaped tracheal morphology, local inflammatory reactions, and infections. To overcome these challenges, this study proposed the addition of icariin (ICA) and chitosan (CS) into a gelatin methacryloyl (GelMA) hydrogel to develop a new ICA/CS/GelMA hydrogel with anti-inflammatory and anti-bacterial properties, and three-dimensional (3D)-bioprinting feasibility. The aim of this study was to construct a TETC, a customized C-shaped cartilage structure, with uniform chondrocyte distribution as well as anti-inflammatory and anti-bacterial functions. Our results confirmed that ICA/CS/GelMA hydrogel provides desirable rheological properties, suitable printability, favorable biocompatibility, and simulated microenvironments for chondrogenesis. Moreover, the addition of ICA stimulated chondrocyte proliferation, extracellular matrix synthesis, and anti-inflammatory ability, while the encapsulation of CS enhanced the hydrogels’ anti-bacterial ability. All these led to the formation of an enhanced TETC after submuscular implantation and an elevated survival rate of experimental rabbits after orthotopic tracheal transplantation. This study provides a reliable cell-laden hydrogel with anti-inflammatory and anti-bacterial activities, suitable printability, and significant advancements in in vivo cartilage regeneration and in situ tracheal cartilage restoration.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"120 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81784096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：