Yuanyuan Chen, Siwei Bi, Jun Gu, Qianli Che, Ruiqi Liu, Wei Li, Tingting Dai, Dongan Wang, Xiaosheng Zhang, Yi Zhang
The global prevalence of diabetes mellitus is experiencing a notable increase. Diabetic patients need to consistently monitor their fluctuating glucose levels caused by the changing diet. Meanwhile, patients with diabetes face a higher risk of developing oral ulcer than healthy individuals. Fortunately, three-dimensional (3D)-printed food, which is design- and texture-customizable, presents a potential solution to alleviate the discomfort caused by ulcer while providing personalized nutrition for patients with unique dietary requirements. In this study, 3D-printable food inks were created based on four food ingredients with low glycemic index, namely milk powder, wheat bran powder, Russula alutacea Fr., (russula mushroom), and Agaricus bisporus (button mushroom) content. Rheological testing and texture profile analysis were performed, affirming that the 3D-printed food possesses a soft texture, which minimizes oral mucosal irritation for patients with diabetic ulcers. The effectiveness of 3D-printed food in diabetes management was corroborated by monitoring the blood glucose levels of streptozotocin-induced diabetic rats via gavage. Food with personalized nutritional composition was custom-printed to cater for the protein requirements of patients with diabetic nephropathy. This innovative approach to personalizing nutrition through 3D food printing has the potential to reshape the future of dietary management, ultimately improving the overall health outcomes and quality of life for individuals with diabetes and its complications.
{"title":"Achieving personalized nutrition for patients with diabetic complications via 3D food printing","authors":"Yuanyuan Chen, Siwei Bi, Jun Gu, Qianli Che, Ruiqi Liu, Wei Li, Tingting Dai, Dongan Wang, Xiaosheng Zhang, Yi Zhang","doi":"10.36922/ijb.1862","DOIUrl":"https://doi.org/10.36922/ijb.1862","url":null,"abstract":"The global prevalence of diabetes mellitus is experiencing a notable increase. Diabetic patients need to consistently monitor their fluctuating glucose levels caused by the changing diet. Meanwhile, patients with diabetes face a higher risk of developing oral ulcer than healthy individuals. Fortunately, three-dimensional (3D)-printed food, which is design- and texture-customizable, presents a potential solution to alleviate the discomfort caused by ulcer while providing personalized nutrition for patients with unique dietary requirements. In this study, 3D-printable food inks were created based on four food ingredients with low glycemic index, namely milk powder, wheat bran powder, Russula alutacea Fr., (russula mushroom), and Agaricus bisporus (button mushroom) content. Rheological testing and texture profile analysis were performed, affirming that the 3D-printed food possesses a soft texture, which minimizes oral mucosal irritation for patients with diabetic ulcers. The effectiveness of 3D-printed food in diabetes management was corroborated by monitoring the blood glucose levels of streptozotocin-induced diabetic rats via gavage. Food with personalized nutritional composition was custom-printed to cater for the protein requirements of patients with diabetic nephropathy. This innovative approach to personalizing nutrition through 3D food printing has the potential to reshape the future of dietary management, ultimately improving the overall health outcomes and quality of life for individuals with diabetes and its complications.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140487953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Shukla, Dongjun Lee, Sik Yoon, Minjun Ahn, Byoung Soo Kim
The skin is composed of many cells that are organized into different layers and connected by dense and complex vascular networks. This creates a dynamic microenvironment in which cells interact within the matrix. Significant advancements have been made in this field over the past decade, and various strategies have been developed for accelerating and enhancing skin regeneration. The primary challenge for successful skin grafts is the integration of the functional vasculature, which can supply essential nutrients and oxygen to cell-laden structures and damaged native tissues. An inadequate vascular network can lead to ischemia, which can cause slow wound healing—particularly in the case of chronic skin conditions. Therefore, blood vessel formation remains one of the most significant obstacles that skin tissue engineering must overcome to create vascularized skin tissue substitutes with specific living cells. Technological advances can augment effective vascularization. The three-dimensional (3D) bioprinting platform is a promising technology that allows precise deposition of living cells and bioactive materials. The application of this technology to skin tissue engineering can provide solutions for augmenting pre-vascularization in engineered in vitro skin models and in vivo skin substitutes. This review presents the significance of skin vascularization in in vitro modeling and in vivo wound healing. Various strategies and related applications involving 3D bioprinting technology are introduced for the biofabrication of enhanced vascularized skin in vitro and in vivo, followed by a discussion of their limitations and future research directions.
{"title":"Vascularization strategies for human skin tissue engineering via 3D bioprinting","authors":"A. Shukla, Dongjun Lee, Sik Yoon, Minjun Ahn, Byoung Soo Kim","doi":"10.36922/ijb.1727","DOIUrl":"https://doi.org/10.36922/ijb.1727","url":null,"abstract":"The skin is composed of many cells that are organized into different layers and connected by dense and complex vascular networks. This creates a dynamic microenvironment in which cells interact within the matrix. Significant advancements have been made in this field over the past decade, and various strategies have been developed for accelerating and enhancing skin regeneration. The primary challenge for successful skin grafts is the integration of the functional vasculature, which can supply essential nutrients and oxygen to cell-laden structures and damaged native tissues. An inadequate vascular network can lead to ischemia, which can cause slow wound healing—particularly in the case of chronic skin conditions. Therefore, blood vessel formation remains one of the most significant obstacles that skin tissue engineering must overcome to create vascularized skin tissue substitutes with specific living cells. Technological advances can augment effective vascularization. The three-dimensional (3D) bioprinting platform is a promising technology that allows precise deposition of living cells and bioactive materials. The application of this technology to skin tissue engineering can provide solutions for augmenting pre-vascularization in engineered in vitro skin models and in vivo skin substitutes. This review presents the significance of skin vascularization in in vitro modeling and in vivo wound healing. Various strategies and related applications involving 3D bioprinting technology are introduced for the biofabrication of enhanced vascularized skin in vitro and in vivo, followed by a discussion of their limitations and future research directions.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140491748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ximin Yuan, Zhenjia Wang, Lixin Che, Xushuai Lv, Jie Xu, D. Shan, Bin Guo
Three-dimensional (3D) bioprinting technologies play significant roles in various facets of the medical field, such as bioengineering, tissue repair, scaffolds, biomedical devices, and drug. As a versatile manufacturing technology, 3D bioprinting is able to overcome the constraints of other conventional methods and shows potential for future advancements in the field of biology. Nevertheless, the existing 3D bioprinting technologies still grapple with significant challenges in materials, equipment, and applications. Therefore, it is essential to select appropriate bioprinting method in alignment with the required application. In this review, we aim to cover the development, classification, and application of 3D bioprinting, with a particular emphasis on the fundamental printing principles. Additionally, we discuss the potential of 3D bioprinting in terms of materialization, structuralization, and functionalization, highlighting its prospective applications. We firmly believe that 3D printing technology will witness widespread adoption in the future, as it has the potential to address the limitations associated with multi-size, multi-material, multi-cell, and high-precision bioprinting.
{"title":"Recent developments and challenges of 3D bioprinting technologies","authors":"Ximin Yuan, Zhenjia Wang, Lixin Che, Xushuai Lv, Jie Xu, D. Shan, Bin Guo","doi":"10.36922/ijb.1752","DOIUrl":"https://doi.org/10.36922/ijb.1752","url":null,"abstract":"Three-dimensional (3D) bioprinting technologies play significant roles in various facets of the medical field, such as bioengineering, tissue repair, scaffolds, biomedical devices, and drug. As a versatile manufacturing technology, 3D bioprinting is able to overcome the constraints of other conventional methods and shows potential for future advancements in the field of biology. Nevertheless, the existing 3D bioprinting technologies still grapple with significant challenges in materials, equipment, and applications. Therefore, it is essential to select appropriate bioprinting method in alignment with the required application. In this review, we aim to cover the development, classification, and application of 3D bioprinting, with a particular emphasis on the fundamental printing principles. Additionally, we discuss the potential of 3D bioprinting in terms of materialization, structuralization, and functionalization, highlighting its prospective applications. We firmly believe that 3D printing technology will witness widespread adoption in the future, as it has the potential to address the limitations associated with multi-size, multi-material, multi-cell, and high-precision bioprinting. ","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140491979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jing Li, Feng Chen, Meixia Wang, Xiaolong Zhu, Ning He, Na Li, Haotian Zhu, Xiaoxiao Han
Light-based 3D printing enables the fabrication of biological scaffolds with high precision, versatility and biocompatibility, particularly the cell-laden scaffolds with architecturally complex geometric features. However, many bioprinted tissue scaffolds suffer from low cell viability due to insufficient oxygen and nutrient supply, which is heavily influenced by scaffold structure and cultivation conditions. Current practice relies mainly on resource-intensive trial-and-error methods to optimize scaffolds’ structures and cultivation parameters. In this study, we developed a comprehensive multi-physics model integrating fluid dynamics, oxygen mass transfer, cell oxygen consumption, and cell growth processes to capture cell growth behaviors in scaffolds, establishing a robust theoretical foundation for scaffold structure optimization. The modeling results showed that a large number of parameters, such as system inlet flow rate, geometric feature size, cell parameters, and material properties, significantly impact oxygen concentration and cell growth within the scaffold. A two-step optimization strategy is proposed in this paper and was applied to obtain optimal geometric parameters of channeled scaffolds to demonstrate the model’s effectiveness for scaffold optimization. The model can be employed for scaffolds with arbitrary shapes and various materials, facilitating the optimal design of sophisticated scaffolds for more advanced tissue engineering.
{"title":"Design and optimization of 3D-bioprinted cell-laden scaffolds in dynamic culture","authors":"Jing Li, Feng Chen, Meixia Wang, Xiaolong Zhu, Ning He, Na Li, Haotian Zhu, Xiaoxiao Han","doi":"10.36922/ijb.1838","DOIUrl":"https://doi.org/10.36922/ijb.1838","url":null,"abstract":"Light-based 3D printing enables the fabrication of biological scaffolds with high precision, versatility and biocompatibility, particularly the cell-laden scaffolds with architecturally complex geometric features. However, many bioprinted tissue scaffolds suffer from low cell viability due to insufficient oxygen and nutrient supply, which is heavily influenced by scaffold structure and cultivation conditions. Current practice relies mainly on resource-intensive trial-and-error methods to optimize scaffolds’ structures and cultivation parameters. In this study, we developed a comprehensive multi-physics model integrating fluid dynamics, oxygen mass transfer, cell oxygen consumption, and cell growth processes to capture cell growth behaviors in scaffolds, establishing a robust theoretical foundation for scaffold structure optimization. The modeling results showed that a large number of parameters, such as system inlet flow rate, geometric feature size, cell parameters, and material properties, significantly impact oxygen concentration and cell growth within the scaffold. A two-step optimization strategy is proposed in this paper and was applied to obtain optimal geometric parameters of channeled scaffolds to demonstrate the model’s effectiveness for scaffold optimization. The model can be employed for scaffolds with arbitrary shapes and various materials, facilitating the optimal design of sophisticated scaffolds for more advanced tissue engineering.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139598005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Min-Hyeok Kim, Jeeyeon Lee, Chwee Teck Lim, Sungsu Park
Human coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), contribute to both respiratory and gastrointestinal symptoms, necessitating a comprehensive approach to studying viral pathogenesis. In this context, bioprinted intestine-on-chip models offer a cutting-edge technology for closely replicating the tissue architecture and microenvironment of the human intestine, providing valuable insights into viral dynamics and host responses. Integration of intestinal organoids with organoid-on-chip technology enhances the accuracy of modeling SARS-CoV-2 infection by means of improving cellular differentiation and virus-binding receptor expression. Furthermore, bioprinting technology allows for automated fabrication, enabling high-throughput drug screening on the intestine-on-chip platform. These advancements in bioprinted intestine-on-chip models hold immense promise for advancing our understanding of coronavirus infection in the gut and accelerating drug development, ultimately contributing to improved patient outcomes and public health measures.
{"title":"Potential of bioprinted intestine-on-chip models in advancing understanding of human coronavirus infections and drug screening","authors":"Min-Hyeok Kim, Jeeyeon Lee, Chwee Teck Lim, Sungsu Park","doi":"10.36922/ijb.1704","DOIUrl":"https://doi.org/10.36922/ijb.1704","url":null,"abstract":"Human coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), contribute to both respiratory and gastrointestinal symptoms, necessitating a comprehensive approach to studying viral pathogenesis. In this context, bioprinted intestine-on-chip models offer a cutting-edge technology for closely replicating the tissue architecture and microenvironment of the human intestine, providing valuable insights into viral dynamics and host responses. Integration of intestinal organoids with organoid-on-chip technology enhances the accuracy of modeling SARS-CoV-2 infection by means of improving cellular differentiation and virus-binding receptor expression. Furthermore, bioprinting technology allows for automated fabrication, enabling high-throughput drug screening on the intestine-on-chip platform. These advancements in bioprinted intestine-on-chip models hold immense promise for advancing our understanding of coronavirus infection in the gut and accelerating drug development, ultimately contributing to improved patient outcomes and public health measures.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139605170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
KyeongWoong Yang, Donghyun Lee, KyoungHo Lee, W. Jang, Hye ji Lim, Eun Ji Lee, Hojun Jeon, Donggu Kang, Gi Hoon Yang, K. Lee, Yong-Il Shin, Sang-Cheol Han, SangHyun An, Sang-Mo Kwon
The inherent limitations of bone grafting in the treatment of critical-sized bone defects have led to a growing demand for bone repair implants. Three-dimensional (3D) bioprinting has emerged as a promising manufacturing technique for implants, offering flexibility in their structural design and the use of applicable materials. Although numerous 3D-bioprinted bone scaffolds have been developed to enhance osteogenesis, angiogenesis remains a challenge. Angiogenesis is crucial for successful bone healing because the process forms blood vessels to deliver essential nutrients and oxygen. Endothelial progenitor cells (EPCs) play a pivotal role in the early stages of vascularization. These cells, capable of differentiating into endothelial cells (ECs), are recruited from the bone marrow to the injured area during the healing process. CD34+ cells, a subset of EPCs, have gained attention because of their neovascularization potential and ability to contribute to bone regeneration. The incorporation of CD34+ cell-enhancing factors into 3D-printed bone scaffolds may facilitate successful bone healing in critical defects. StemRegenin-1 (SR1), a molecule that promotes CD34+ cell expansion, has shown promising results in increasing CD34+ hematopoietic stem and progenitor cell populations. This study aimed to investigate the sustained release of SR1 from a collagen-based scaffold integrated with mesoporous silica nanoparticles (MSNs) to promote angiogenesis and enhance bone healing. The sustained release of SR1 from the collagen scaffold is hypothesized to promote angiogenesis, thereby facilitating bone repair. In vitro studies have demonstrated the angiogenic potential of SR1; however, further in vivo investigations are required to establish its clinical efficacy. This study contributes to the development of novel therapies targeting CD34+ cells and demonstrates the potential of SR1 as a promising agent for promoting angiogenesis and enhancing bone healing in critical defects.
{"title":"3D-bioprinted bone scaffolds incorporating SR1 nanoparticles enhance blood vessel regeneration in rat calvarial defects","authors":"KyeongWoong Yang, Donghyun Lee, KyoungHo Lee, W. Jang, Hye ji Lim, Eun Ji Lee, Hojun Jeon, Donggu Kang, Gi Hoon Yang, K. Lee, Yong-Il Shin, Sang-Cheol Han, SangHyun An, Sang-Mo Kwon","doi":"10.36922/ijb.1931","DOIUrl":"https://doi.org/10.36922/ijb.1931","url":null,"abstract":"The inherent limitations of bone grafting in the treatment of critical-sized bone defects have led to a growing demand for bone repair implants. Three-dimensional (3D) bioprinting has emerged as a promising manufacturing technique for implants, offering flexibility in their structural design and the use of applicable materials. Although numerous 3D-bioprinted bone scaffolds have been developed to enhance osteogenesis, angiogenesis remains a challenge. Angiogenesis is crucial for successful bone healing because the process forms blood vessels to deliver essential nutrients and oxygen. Endothelial progenitor cells (EPCs) play a pivotal role in the early stages of vascularization. These cells, capable of differentiating into endothelial cells (ECs), are recruited from the bone marrow to the injured area during the healing process. CD34+ cells, a subset of EPCs, have gained attention because of their neovascularization potential and ability to contribute to bone regeneration. The incorporation of CD34+ cell-enhancing factors into 3D-printed bone scaffolds may facilitate successful bone healing in critical defects. StemRegenin-1 (SR1), a molecule that promotes CD34+ cell expansion, has shown promising results in increasing CD34+ hematopoietic stem and progenitor cell populations. This study aimed to investigate the sustained release of SR1 from a collagen-based scaffold integrated with mesoporous silica nanoparticles (MSNs) to promote angiogenesis and enhance bone healing. The sustained release of SR1 from the collagen scaffold is hypothesized to promote angiogenesis, thereby facilitating bone repair. In vitro studies have demonstrated the angiogenic potential of SR1; however, further in vivo investigations are required to establish its clinical efficacy. This study contributes to the development of novel therapies targeting CD34+ cells and demonstrates the potential of SR1 as a promising agent for promoting angiogenesis and enhancing bone healing in critical defects.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139612355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ying Shan, Mingchang Pang, Liqian Wang, Yixin Mao, Ruiyi Yan, Chang Zhou, Jingyuan Ji, Yilei Mao, Ying Jin, Huayu Yang
Ovarian cancer is a gynecological malignancy with a high mortality rate. The ovarian cancer microenvironment is a crucial factor affecting the overall and progression-free survival rates of patients with ovarian cancer. The biophysical factors of the tumor microenvironment, such as stiffness, can affect the gene expression and behavior of tumor cells. In this study, we utilized 3D bioprinting technology to construct ovarian cancer tumor models with varying levels of stiffness in vitro to investigate the effect of extracellular matrix stiffness on drug resistance of tumor cells. Our findings indicate that increasing the stiffness of extracellular matrix can attenuate the sensitivity of tumor cells to chemotherapeutic agents. Additionally, the increased stiffness of 3D tumor model may promote malignant phenotypes, such as tumor stemness and tumor progression.
{"title":"Increased stiffness of extracellular matrix enhanced chemoresistance in 3D-bioprinted ovarian cancer model","authors":"Ying Shan, Mingchang Pang, Liqian Wang, Yixin Mao, Ruiyi Yan, Chang Zhou, Jingyuan Ji, Yilei Mao, Ying Jin, Huayu Yang","doi":"10.36922/ijb.1673","DOIUrl":"https://doi.org/10.36922/ijb.1673","url":null,"abstract":"Ovarian cancer is a gynecological malignancy with a high mortality rate. The ovarian cancer microenvironment is a crucial factor affecting the overall and progression-free survival rates of patients with ovarian cancer. The biophysical factors of the tumor microenvironment, such as stiffness, can affect the gene expression and behavior of tumor cells. In this study, we utilized 3D bioprinting technology to construct ovarian cancer tumor models with varying levels of stiffness in vitro to investigate the effect of extracellular matrix stiffness on drug resistance of tumor cells. Our findings indicate that increasing the stiffness of extracellular matrix can attenuate the sensitivity of tumor cells to chemotherapeutic agents. Additionally, the increased stiffness of 3D tumor model may promote malignant phenotypes, such as tumor stemness and tumor progression.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139615755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shaojun Liang, Yixue Luo, Yijun Su, Dawei Zhang, Shi-jie Wang, Mingen Xu, Rui Yao
Bioprinted tissues derived from human-induced pluripotent stem cells (hiPSCs) can provide precise information on disease mechanisms and toxicity. The detection of microplastics (MPs) in the liver tissues of patients with liver cirrhosis has raised concerns about their hepatotoxicity. MPs could absorb endocrine disruptors, such as tetrabromobisphenol A (TBBPA) that is widely present in the environment, thereby complicating their toxic behaviors. To investigate their toxic mechanisms in liver tissues, we used the electro-assisted inkjet printing technology to fabricate healthy donor or patient-sourced hiPSC-derived Disse space organoids (DOs) that resembled the cell types and transcriptional features of Disse space. We observed an accumulation of polystyrene MP microbeads in the DOs, and TBBPA exacerbated the process. Neither MPs and TBBPA alone nor the co-exposure at non-cytotoxicity dosages could affect the liver functions of healthy donor hiPSC-derived DOs, as revealed by transcriptomic and biochemical analyses, whereas alcoholic liver disease (ALD) patient hiPSC-derived DOs exhibited the ALD disease transcriptional profiles. We found that MPs/TBBPA co-exposure significantly influenced the patient organoids in terms of the pathological transcription expression and biochemical profiles. These results suggested that both hereditary factors and pollutants contribute to susceptibility to environmental toxicants. This study exemplified the value of bioprinting hiPSC-derived organoids in environmental toxicology, offering a powerful strategy to advance the personalized environmental toxicology paradigm.
{"title":"Distinct toxicity of microplastics/TBBPA co-exposure to bioprinted liver organoids derived from hiPSCs of healthy and patient donors","authors":"Shaojun Liang, Yixue Luo, Yijun Su, Dawei Zhang, Shi-jie Wang, Mingen Xu, Rui Yao","doi":"10.36922/ijb.1403","DOIUrl":"https://doi.org/10.36922/ijb.1403","url":null,"abstract":"Bioprinted tissues derived from human-induced pluripotent stem cells (hiPSCs) can provide precise information on disease mechanisms and toxicity. The detection of microplastics (MPs) in the liver tissues of patients with liver cirrhosis has raised concerns about their hepatotoxicity. MPs could absorb endocrine disruptors, such as tetrabromobisphenol A (TBBPA) that is widely present in the environment, thereby complicating their toxic behaviors. To investigate their toxic mechanisms in liver tissues, we used the electro-assisted inkjet printing technology to fabricate healthy donor or patient-sourced hiPSC-derived Disse space organoids (DOs) that resembled the cell types and transcriptional features of Disse space. We observed an accumulation of polystyrene MP microbeads in the DOs, and TBBPA exacerbated the process. Neither MPs and TBBPA alone nor the co-exposure at non-cytotoxicity dosages could affect the liver functions of healthy donor hiPSC-derived DOs, as revealed by transcriptomic and biochemical analyses, whereas alcoholic liver disease (ALD) patient hiPSC-derived DOs exhibited the ALD disease transcriptional profiles. We found that MPs/TBBPA co-exposure significantly influenced the patient organoids in terms of the pathological transcription expression and biochemical profiles. These results suggested that both hereditary factors and pollutants contribute to susceptibility to environmental toxicants. This study exemplified the value of bioprinting hiPSC-derived organoids in environmental toxicology, offering a powerful strategy to advance the personalized environmental toxicology paradigm.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tamás Monostori, Diána Szűcs, Borbála Lovászi, Lajos Kemény, Zoltán L Veréb
Blindness resulting from corneal damage affects millions of people worldwide. The scarcity of corneal donors adds a layer of complexity to patient treatment. Consequently, exploring artificial cornea substitutes has become imperative in the realm of clinical research. Scientific advancements have ushered in a plethora of innovative solutions, including keratoprostheses or decellularized cornea scaffolds. The development of three-dimensional (3D) printing has further expanded the horizons of research in this field, delving into the feasibility of bioprinted corneas and yielding numerous promising outcomes. However, the manufacturing of corneal products via 3D printing poses a substantial challenge, demanding a meticulous selection of materials and techniques to ensure the transparency and preservation of the optical and mechanical properties of the artificial cornea. In the review, we present the artificial cornea substitutes. Additionally, we aim to provide a concise overview of the 3D printing techniques and materials applicable to corneal bioprinting.
{"title":"Advances in tissue engineering and 3D bioprinting for corneal regeneration","authors":"Tamás Monostori, Diána Szűcs, Borbála Lovászi, Lajos Kemény, Zoltán L Veréb","doi":"10.36922/ijb.1669","DOIUrl":"https://doi.org/10.36922/ijb.1669","url":null,"abstract":"Blindness resulting from corneal damage affects millions of people worldwide. The scarcity of corneal donors adds a layer of complexity to patient treatment. Consequently, exploring artificial cornea substitutes has become imperative in the realm of clinical research. Scientific advancements have ushered in a plethora of innovative solutions, including keratoprostheses or decellularized cornea scaffolds. The development of three-dimensional (3D) printing has further expanded the horizons of research in this field, delving into the feasibility of bioprinted corneas and yielding numerous promising outcomes. However, the manufacturing of corneal products via 3D printing poses a substantial challenge, demanding a meticulous selection of materials and techniques to ensure the transparency and preservation of the optical and mechanical properties of the artificial cornea. In the review, we present the artificial cornea substitutes. Additionally, we aim to provide a concise overview of the 3D printing techniques and materials applicable to corneal bioprinting.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139619989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mihyeon Bae, Joeng Ju Kim, Jongmin Kim, Dong-Woo Cho
Precise in vitro models in tissue engineering have attracted the attention of researchers seeking to understand physiological consequences from native tissues as well as the mechanism of diseases in vitro. To construct delicate native tissue-like in vitro models, a proper combination of biomimetic materials and a biofabrication strategy is required. Conventional biomaterials, such as collagens, laminins, and synthetic polymers, have been widely adapted in tissue recapitulation; however, they lack tissue specificity in the context of biophysical properties and native-like extracellular matrix composition. The lack of tissue specificity accounts for the pathophysiological discrepancy between preclinical model and actual human patient. Thus, biomaterials should be improved for attaining physiological similarity between disease models and patients. Additionally, a biofabrication technique is essential for building mature cellular or tissue structures with a sophisticated bioassembly process. Among the biofabrication techniques, bioprinting stands as a promising approach for constructing three-dimensional (3D) cellular structures using specific cell types and biomaterials. Combining multifunctional bioinks and bioprinting is expected to enhance tissue specificity with regard to structural recapitulation. From this viewpoint, decellularized extracellular matrix (dECM) bioink has been increasingly used to achieve tissue specificity and manufacturability in 3D bioprinting. Progress in this domain requires the clarification of tissue-specific decellularization method and the development of a proper 3D bioprinting method, in conjunction with the improvement of the compatibility between dECM and bioprinting. In this review, we introduce the production methods and characteristics of dECM in the context of tissue specificity and examine state-of-the-art dECM-incorporated 3D-bioprinted in vitro models for disease investigation. We also recommend a strategy for improving dECM for use in therapeutic studies based on simulations of the pathophysiological microenvironment.
{"title":"Decellularized extracellular matrix for three-dimensional bioprinted in vitro disease modeling","authors":"Mihyeon Bae, Joeng Ju Kim, Jongmin Kim, Dong-Woo Cho","doi":"10.36922/ijb.1970","DOIUrl":"https://doi.org/10.36922/ijb.1970","url":null,"abstract":"Precise in vitro models in tissue engineering have attracted the attention of researchers seeking to understand physiological consequences from native tissues as well as the mechanism of diseases in vitro. To construct delicate native tissue-like in vitro models, a proper combination of biomimetic materials and a biofabrication strategy is required. Conventional biomaterials, such as collagens, laminins, and synthetic polymers, have been widely adapted in tissue recapitulation; however, they lack tissue specificity in the context of biophysical properties and native-like extracellular matrix composition. The lack of tissue specificity accounts for the pathophysiological discrepancy between preclinical model and actual human patient. Thus, biomaterials should be improved for attaining physiological similarity between disease models and patients. Additionally, a biofabrication technique is essential for building mature cellular or tissue structures with a sophisticated bioassembly process. Among the biofabrication techniques, bioprinting stands as a promising approach for constructing three-dimensional (3D) cellular structures using specific cell types and biomaterials. Combining multifunctional bioinks and bioprinting is expected to enhance tissue specificity with regard to structural recapitulation. From this viewpoint, decellularized extracellular matrix (dECM) bioink has been increasingly used to achieve tissue specificity and manufacturability in 3D bioprinting. Progress in this domain requires the clarification of tissue-specific decellularization method and the development of a proper 3D bioprinting method, in conjunction with the improvement of the compatibility between dECM and bioprinting. In this review, we introduce the production methods and characteristics of dECM in the context of tissue specificity and examine state-of-the-art dECM-incorporated 3D-bioprinted in vitro models for disease investigation. We also recommend a strategy for improving dECM for use in therapeutic studies based on simulations of the pathophysiological microenvironment.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139619255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：