Rhizosphere最新文献

{"title":"Arbuscular mycorrhizal symbiosis Drives rhizosphere-regulated drought tolerance in maize","authors":"Ghulam Murtaza ,&nbsp;Muhammad Usman ,&nbsp;Khairiah Mubarak Alwutayd ,&nbsp;Rashid Iqbal","doi":"10.1016/j.rhisph.2025.101250","DOIUrl":"10.1016/j.rhisph.2025.101250","url":null,"abstract":"<div><div>Drought-induced stress is a significant constraint for crop yields in semi-arid and arid areas.</div><div>Yield assessments under water stress indicate that mycorrhizae can alleviate the detrimental impacts of drought, placing them as sustainable options for agricultural practices in affected areas. Thus, we executed a two-year study to examine the effects of root colonization by two AMF species (<em>Diversispora epigaea</em> and <em>Diversispora versiformis</em>) under different drought stress conditions, assessing maize morpho-physiological and biochemical characteristics, nutrient absorption, yield components, oil percentage, and irrigation water efficiency. The research was conducted in a desolate region of Pakistan during the 2023 and 2024 growing seasons. Drought-induced stress was generated at two levels by irrigating after 80 % and 60 % water loss, categorized as severe and mild drought stress. Irrigation after a 40 % reduction in water was considered normal (without stress). The findings demonstrated that regardless of AMF species and level of drought stress, inoculated plants yielded heavier seeds, higher dry matter, chlorophyll (37 %) and carotenoids (41 %), phytohormone (27 %), enhanced oil yields (32 %) and seeds (24.2 %) compared to uninoculated plants. Notably, the maize seed yields of <em>Diversispora epigaea</em>-treated plants under every irrigation treatment surpassed those of <em>Diversispora versiformis</em> inoculated plants and uninoculated plants. Drought stress reduced nitrogen levels in seeds and leaves, whereas AMF enhanced nitrogen levels, particularly when crops were treated with <em>Diversispora epigaea</em>. Moreover, seed phosphorus percentages were not influenced by AMF in 2023. Conversely, the highest phosphorus percentages in seeds and leaves were recorded in crops inoculated with <em>Diversispora epigaea</em> in 2023. Our findings indicate that <em>Diversispora epigaea</em> exhibits greater efficiency under water stress and provides superior support to maize plants.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101250"},"PeriodicalIF":3.5,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145841152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cd-immobilizing strain combined with pig manure biochar regulates rhizosphere microecology to reduce Cd absorption by wheat","authors":"Zhipeng Wang ,&nbsp;Xiaofei Liu ,&nbsp;Tao Peng ,&nbsp;Shasha Huang ,&nbsp;Weifeng Zhao ,&nbsp;Li Chen ,&nbsp;Hui Han","doi":"10.1016/j.rhisph.2025.101249","DOIUrl":"10.1016/j.rhisph.2025.101249","url":null,"abstract":"<div><div>Soil functional microorganisms and biochar regulate the chemical speciation of Cd, thereby influencing its uptake by crops. However, the effects of Cd-immobilizing bacteria combined with biochar on Cd speciation in wheat fields and subsequent Cd accumulation in wheat remain unclear. This study investigated the impacts and underlying soil mechanisms of applying the urease-producing bacterium <em>Enterobacter</em> sp. TJ6 and pig manure biochar (PMB) on wheat growth and Cd uptake using pot experiments. Results demonstrated that the combined application of TJ6 and PMB increased (10.8 %) wheat grain dry weight and reduced (72.1 %) grain Cd concentration compared to the control. This reduction was primarily attributed to the conversion of bioavailable Cd in the rhizosphere soil into organically-bound and residual Cd fractions. Increaseing soil pH, electrical conductivity, NH₄⁺ content, NH₄⁺/NO₃⁻ ratio, and urease activity promoted the immobilization of Cd onto soil particles, consequently decreasing the concentration of bioavailable Cd. Furthermore, the TJ6+PMB amendment facilitated the formation of insoluble Cd precipitates, such as Cd₃(PO₄)₂, CdCO₃, and (Cd,Ca)₅(PO₄)₃OH, within the rhizosphere soil. Compared to the fungal community, the bacterial community in the rhizosphere exhibited greater sensitivity to TJ6+PMB application. The relative abundances of key bacterial genera, including <em>Sphingomonas, Bacillus, Gemmatimonas,</em> and <em>Nocardioides,</em> were significantly enhanced. These genera play crucial roles in heavy metal immobilization, plant growth promotion, and nitrogen cycling. In conclusion, the application of strain TJ6 and PMB effectively reduced Cd bioavailability and uptake by wheat through the regulation of rhizosphere soil physicochemical properties and microbial community structure. These findings provide a practical approach for the remediation and safe production of wheat in Cd-contaminated fields.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101249"},"PeriodicalIF":3.5,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative regulatory networks modulating arbuscular mycorrhizal symbiosis","authors":"Yaseen Khan ,&nbsp;Sulaiman Shah ,&nbsp;Muhammad Faheem Jan ,&nbsp;Mohammed Bouskout","doi":"10.1016/j.rhisph.2025.101248","DOIUrl":"10.1016/j.rhisph.2025.101248","url":null,"abstract":"<div><div>Arbuscular mycorrhizal symbiosis plays a pivotal role in nutrient acquisition and stress tolerance, making its regulation crucial for sustainable crop productivity. This review synthesizes current advances in understanding the molecular and physiological factors governing AM symbiosis, with emphasis on transcriptional, hormonal, and nutrient-mediated regulation. From pre-symbiotic signaling to root colonization and arbuscule development, AM formation is orchestrated by a complex network of molecular interactions. Transcription factors, including those with GRAS domains (e.g., NSP1, NSP2, RAM1, and DELLA), and other regulators such as MYB, SPX, WRKY, and CYCLOPS/IPD3, serve as central modulators of symbiosis-related gene expression. Phytohormones, including strigolactones, salicylic acid, and abscisic acid, generally promote symbiosis, whereas gibberellins and ethylene act as inhibitors; cytokinin exerts context-dependent effects. Nutrient status also modulates AM formation—low phosphorus and nitrogen promote, while high nutrient availability suppresses colonization. Collectively, these insights reveal the integrative regulatory networks driving AM symbiosis and offer new avenues to optimize symbiotic efficiency for enhanced plant growth and agricultural sustainability.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101248"},"PeriodicalIF":3.5,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Connecting soil spatial heterogeneities and grapevine root system architecture in California's Central Valley using non-invasive geophysical methods","authors":"Solomon Ehosioke ,&nbsp;Sam Dudley ,&nbsp;Andrew J. McElrone ,&nbsp;Megan Bartlett ,&nbsp;Gordon Osterman","doi":"10.1016/j.rhisph.2025.101247","DOIUrl":"10.1016/j.rhisph.2025.101247","url":null,"abstract":"<div><div>Soil spatial heterogeneity significantly impacts grapevine performance and water-use efficiency, yet understanding the intricate belowground dynamics remains a challenge. This study integrates non-invasive geophysical methods with physiological measurements and root morphology analysis to investigate the influence of soil variability on two grapevine rootstocks, Millardet et de Grasset 101-14 (101-14 Mgt) and Richter 110 (110R), in California's Central Valley. Electromagnetic induction and electrical resistivity tomography were used to map soil spatial heterogeneity, revealing distinct soil units (Yolo silt loam and Reiff very fine sandy loam) with varying physical and hydraulic properties. Electrical capacitance measurement successfully evaluated root system size as we found a positive correlation between root electrical capacitance and root dry mass in both rootstocks. Our results also show that root capacitance was consistently higher in the high vigor and drought resistant 110R than in the moderate vigor and drought sensitive 101-14, highlighting root system differences between the rootstocks. Furthermore, we found that vine water status, indicated by stem water potential and stomatal conductance, varied significantly between rootstocks and locations, and are driven by the underlying soil properties. This research demonstrates the utility of integrating geophysical and electrical methods for field-based phenotyping, providing novel insights into the soil-plant continuum. These findings highlight the potential of agrogeophysics for characterizing vineyard spatial heterogeneity to inform site-specific vineyard management, optimize irrigation strategies, and aid in rootstock selection for enhanced drought resilience without the need for labor intensive excavations.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101247"},"PeriodicalIF":3.5,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wheat density drives negative density dependence in nitrogen uptake and root plasticity in wheat–weed communities","authors":"Li Zhang ,&nbsp;Weiqiang Liu ,&nbsp;Yizhong Rong ,&nbsp;Jiazhen Xi ,&nbsp;Lingfeng Mao ,&nbsp;Anna Gunina ,&nbsp;Zhen Zhang","doi":"10.1016/j.rhisph.2025.101243","DOIUrl":"10.1016/j.rhisph.2025.101243","url":null,"abstract":"<div><div>Adverse density-dependent effects on competition outcomes (e.g., fecundity) are stronger within species than between them, promoting species coexistence. However, empirical evidence on the resource competition process (e.g., nitrogen, N) and on how plant root traits and fertilization alter this process remains unclear. We conducted a response-surface design competition experiment involving wheat and weeds (<em>Avena fatua</em> L. or <em>Echinochloa crus-galli</em> (L.) P. Beauv., i.e., WB and WO pair) across densities (4, 8, 12, 16 individuals per pot) and proportions (wheat:weed 0:1, 0.25:0.75, 0.5:0.5, 0.75:0.25, 1:0), and fertilization treatment. After seven months, root morphology traits were measured and NH₄⁺ and NO₃⁻ uptake was studied using short-term ¹⁵N labeling. Without fertilization, high wheat density reduced wheat's root area (α_<em>ii</em> = −0.130 and −0.147 in WB and WO pairs) and length (−0.110; −0.120) while increasing specific root length (0.040; 0.062) and area (0.019; 0.035) to mitigate a reduction in NH₄⁺ (−0.142) than NO₃⁻ (−0.205). Weeds reduced root length and NH₄⁺ uptake. With fertilization, increasing wheat density decreased wheat and weed root length (α_<em>ii</em> = −0.117, α_<em>ij</em> = −0.238 in WB pair; α_<em>ii</em> = −0.117, α_<em>ij</em> = −0.181 in WO pair) and area (α_<em>ii</em> = −0.126, α_<em>ij</em> = −0.283; α_<em>ii</em> = −0.152, α_<em>ij</em> = −0.206), NH₄⁺ (α_<em>ii</em> = −0.281; α_<em>ij</em> = −0.224), and NO₃⁻ uptake (α_<em>ii</em> = −0.079; α_<em>ij</em> = −0.326). Weeds also increased specific root length (0.220 and 0.079) and area (0.054 in the WO pair). These N reduction and root plasticity were not observed with increased weed density, suggesting that maintaining weed presence did not reduce wheat's N uptake. Our research indicated that weed management strategies should consider density-dependent N competition and root plasticity within agricultural systems.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101243"},"PeriodicalIF":3.5,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoupled adaptation of plant microbiomes under global change: Partitioned responses and functional plasticity to warming and herbivory","authors":"Wenjing Chen ,&nbsp;Kelu Chen ,&nbsp;Huakun Zhou ,&nbsp;Li Ma","doi":"10.1016/j.rhisph.2025.101239","DOIUrl":"10.1016/j.rhisph.2025.101239","url":null,"abstract":"<div><h3>Background</h3><div>Multiple global change drivers frequently co-occur and interact, complicating predictions of ecosystem responses. However, an integrated understanding of how plant-associated microbiomes—spanning distinct host compartments and ecological niches—adjust to simultaneous climatic and biotic pressures remains limited.</div></div><div><h3>Results</h3><div>We conducted a randomized block split-plot field experiment in an alpine meadow, incorporating warming, simulated herbivory, and their combination, to examine bacterial and fungal communities associated with leaves and roots across both exogenous and endophytic fractions. High-throughput sequencing enabled a comprehensive evaluation of changes in community structure (diversity and composition), functional potential (predicted via PICRUSt2 and FUNGuild), and co-occurrence network characteristics. Three consistent patterns emerged: (i) marked spatially partitioned responses, wherein phyllosphere microbiomes—particularly exogenous assemblages—displayed high sensitivity to stressors (e.g., increased diversity, network simplification), whereas rhizosphere microbiomes exhibited greater resilience; (ii) community variation was driven predominantly by non-linear interactions between warming and herbivory rather than additive effects, with synergistic or antagonistic outcomes strongly dependent on the niche and ecological dimension; and (iii) a pervasive decoupling among community structure, functional potential, and network stability. For example, root endophytic networks were largely insensitive to combined stressors, yet predicted functional gene profiles changed markedly; conversely, leaf endophytic diversity remained stable even as functional potential was substantially reshaped.</div></div><div><h3>Conclusions</h3><div>These findings support an integrated “adaptive decoupling” framework, proposing that structural–functional decoupling serves as a central strategy enabling plant microbiomes to maintain resilience under multiple stressors. This mechanism allows communities to rapidly adjust functional capacities without altering overall structure, or to preserve core network stability during structural reorganization, thereby conferring substantial functional plasticity. The results challenge the conventional “structure determines function” paradigm and offer a more mechanistic basis for predicting ecosystem functional stability under accelerating global change.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101239"},"PeriodicalIF":3.5,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycorrhizal consortium: an option to modulate leaf lectin biosynthesis","authors":"Caio Bezerra Barreto ,&nbsp;Francisco Chagas Barbalho Neto ,&nbsp;Carmelo José Albanez Bastos-Filho ,&nbsp;Qiang-Sheng Wu ,&nbsp;Michele Dalvina Correia da Silva ,&nbsp;Fábio Sérgio Barbosa da Silva","doi":"10.1016/j.rhisph.2025.101242","DOIUrl":"10.1016/j.rhisph.2025.101242","url":null,"abstract":"<div><div>The association with arbuscular mycorrhizal fungi (AMF) can modulate the production of both primary and secondary metabolites in plants. Although the importance of root lectins in establishing symbiosis is well recognized, nevertheless, it is not known whether mycorrhizal inoculation can modulate the accumulation of leaf lectins. This study aimed to verify whether the inoculation of a mycorrhizal consortium alters the lectins profile in leaves. A greenhouse experiment with two inoculation treatments was carried out: non-inoculated <em>Schinus terebinthifolia</em> Raddi seedlings (control) and seedlings inoculated with an AMF consortium containing <em>Acaulospora longula</em>, <em>Entrophospora etunicata,</em> and <em>Dentiscutata heterogama</em> (AMF+). The leaves were harvested after 191 days and used to prepare aqueous extracts. The extracts were assayed regarding the hemagglutinating activity, for detecting lectins, and the specific hemagglutinating activity (SHA) was determined for ABO group erythrocytes. The concentration of bioatives and the <em>in vitro</em> antioxidant activity were evaluated. The SHA of leaves from mycorrhizal <em>S</em>. <em>terebinthifolia</em> seedlings, evaluated using group A erythrocytes, was enhanced by over 30%, in comparison with non-inoculated plants (<em>p≤ </em>0.01). However, inoculation of AMF reduced the production of metabolites, the antioxidant activity, and SHA when erythrocytes from groups AB, B, or O were considered (<em>p≤ </em>0.01). This research provides the first evidence of mycorrhizal symbiosis affecting leaf lectin accumulation.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101242"},"PeriodicalIF":3.5,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant species and altitudinal gradients jointly shape rhizosphere bacterial community structure in mountain ecosystems","authors":"Yixin Song ,&nbsp;Tian Zhang ,&nbsp;Maryamgul Yasen ,&nbsp;Mingyuan Li ,&nbsp;Jilian Wang","doi":"10.1016/j.rhisph.2025.101245","DOIUrl":"10.1016/j.rhisph.2025.101245","url":null,"abstract":"<div><div>In mountainous systems, elevation gradients regulate soil properties and plant distribution. Rhizosphere bacteria are key mediators of soil-plant interactions, and their altitudinal variation and host association are core to deciphering the adaptation mechanisms of mountainous ecosystems. In our study, 16S amplicon sequencing was employed to analyze the rhizosphere soil bacterial community structures of different plant species along altitude gradients (1500–4000 m) on the eastern Pamir Plateau, China. The three plant species include <em>Cirsium japonicum</em>, <em>Phragmites australis</em>, and <em>Medicago sativa</em>. The results showed the richness and diversity of bacteria ranked in the order <em>M</em>. <em>sativa</em>, <em>P</em>. <em>australis</em>, <em>and C</em>. <em>japonicum</em>, and the response to altitude varied among plant species. Proteobacteria (34.2 %–41.5 %) and Actinobacteriota (22.8 %–28.6 %) were the dominant phyla, and the bacterial communities were sensitive to altitude changes. The functions of bacteria inferred from 16S data were dominated by metabolism (45.5 %–49.8 %) and genetic information processing (20.4 %–22.3 %), and also exhibited plant-altitude-specific differentiation. Only the bacterial diversity of <em>C</em>. <em>japonicum</em> and <em>P</em>. <em>australis</em> was correlated with soil nutrients, while no significant correlation was observed for <em>M</em>. <em>sativa</em>. Moreover, the correlation between bacterial functions and the rhizosphere bacteriota, as well as soil nutrients, varied among plant species. The metabolic functions were enhanced at 3500 m and 4000 m, and the genetic information processing function of <em>M</em>. <em>sativa</em> was prominent. The partial least squares path model further confirmed the unique altitude adaptation strategies of the three plant species. This study provides data support for deciphering the altitudinal adaptation mechanisms of alpine plant-microbe interactions.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101245"},"PeriodicalIF":3.5,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic regulation of calcium hydroxide and ARC microbial inoculant on rhizosphere microbiota and soil chemical properties during peanut middle growth stages in acidic red soils","authors":"Gongming Wu ,&nbsp;Hong Yu ,&nbsp;Ningbo Zeng ,&nbsp;Zinan Luo ,&nbsp;Sheng Gao ,&nbsp;Qi Liang ,&nbsp;Lin Li ,&nbsp;Peiwu Li ,&nbsp;Zemao Yang ,&nbsp;Dengwang Liu","doi":"10.1016/j.rhisph.2025.101238","DOIUrl":"10.1016/j.rhisph.2025.101238","url":null,"abstract":"<div><div>To address the high acidity and low fertility of acidic red soils in southern China, this study evaluated the effects of calcium hydroxide (Ca(OH)₂) and the <em>Aspergillus</em>-Rhizobia Coupling (ARC) microbial inoculant on rhizosphere microbiota, soil chemical properties, and peanut <em>(Arachis hypogaea</em> L.) yield during the middle growth stage of peanuts (i.e., flowering-pegging and pod-setting stages). Calcium hydroxide modulated the rhizosphere microenvironment by increasing soil pH and exchangeable calcium, thereby enhancing microbial α-diversity, stimulating the metabolic activity of both ARC inoculant strains and native microbiota, and enriching carbon and nitrogen (C-N) cycling-related taxa (e.g., <em>Sphingomonas</em>), thus elevating soil organic matter and hydrolyzable nitrogen, and ultimately increasing peanut total pod weight per plant. Meanwhile, the ARC inoculant reshaped the rhizosphere microbial community structure without altering α-diversity, specifically enriching phosphorus and potassium (P-K) activation-related taxa (e.g., <em>Paraglomus</em>), thereby increasing soil available phosphorus and available potassium, and ultimately enhancing peanut total pods per plant. The combined application of these amendments exhibited obvious synergistic effects in the above aspects, with the Ca₅₀A₄ treatment (750 kg/ha calcium hydroxide + 60 kg/ha ARC inoculant) achieving the greatest effects. Compared with the control (Ca₀A₀, CK), the total pods per plant and total pod weight per plant in the Ca₅₀A₄ treatment were significantly increased by 57.14 % and 45.18 %, respectively. Overall, calcium hydroxide improved the rhizosphere microhabitat primarily through chemical regulation, whereas the ARC inoculant acted via direct and community-mediated biological regulation. Their synergistic application provides an efficient and innovative strategy for improving soil quality and enhancing peanut productivity in acidic red soil regions of southern China.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101238"},"PeriodicalIF":3.5,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145694921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in rhizospheric bacterial communities of the Andean shrub Fabiana densa in response to salinity","authors":"Cinthia Copeticona-Callejas,&nbsp;Isabel Morales-Belpaire","doi":"10.1016/j.rhisph.2025.101244","DOIUrl":"10.1016/j.rhisph.2025.101244","url":null,"abstract":"<div><div>The expansion of quinoa cultivation areas in the Bolivian Altiplano has diminished the populations of native plants which play key ecological roles in the harsh environments of the high Andes. The shrub <em>Fabiana densa</em> not only thrives under extreme climatic conditions and nutrient-poor soils, but also shows tolerance to salinity. Among these species, the shrub <em>Fabiana densa</em> not only thrives under extreme climatic conditions and nutrient-poor soils, but it also shows tolerance to salinity. Understanding the adaptation mechanisms of <em>F. densa</em> to salinity could help revegetate salt-affected areas. Rhizobacteria, among other factors, may contribute to salinity tolerance of <em>F. densa</em>. Therefore, we aimed to assess how salinity affects the rhizospheric bacterial communities of <em>F. densa</em>, as a first step toward identifying bacterial families potentially involved in saline stress alleviation. We irrigated <em>F. densa</em> plants with 0, 15, 25, and 40 mM NaCl solutions under controlled conditions. After ten and twenty weeks of exposure to the salinity treatments, DNA was extracted from rhizospheric soil. The bacterial communities were analyzed by high-throughput sequencing of the V4 region of the 16S rRNA gene. Both salinity level and exposure time had a strong effect on the composition of the rhizospheric bacterial communities. After 10 weeks of exposure, differential abundances of Cyclobacteriaceae and Shewanellaceae were positively related to salinity. After 20 weeks of exposure, salinity caused a decrease in the differential abundances of Aeromonaceae and Rhodocyclaceae but an increase for Rhizobiaceae. The changes in community composition with time of sampling suggest that besides exposure to salinity, other environmental factors influenced bacterial communities and should be taken into account in further studies.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101244"},"PeriodicalIF":3.5,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}