Rhizosphere最新文献

{"title":"Crop domestication has increased phosphorus-acquisition capacity but restricted root plasticity under phosphorus-limited conditions","authors":"Ning-Jiao Ma ,&nbsp;Ying-Qian Zhang ,&nbsp;Lin-Wei Xu ,&nbsp;Jiayin Pang ,&nbsp;Yinglong Chen ,&nbsp;Qian Xu ,&nbsp;Jin He ,&nbsp;Hans Lambers","doi":"10.1016/j.rhisph.2025.101087","DOIUrl":"10.1016/j.rhisph.2025.101087","url":null,"abstract":"<div><div>Crop domestication marks a crucial milestone in human civilization, yet our understanding of the changes in belowground crop traits, specifically those associated with phosphorus (P)-acquisition strategies, during domestication remains limited. Ten crop species and their wild relatives were grown under both P-sufficient (40 mg P kg⁻¹) and P-deficient (nil-P added) conditions to compare the biomass, P content and P-acquisition strategies related to root morphological and physiological traits. Domesticated crops presented a lower specific root length and acid phosphatase activity but greater arbuscular mycorrhizal (AM) colonization, more root-secreted carboxylates per plant, and higher values for root morphological traits such as root length than their wild relatives. Domesticated crops showed a 79 % higher P content than their wild relatives under P-deficient conditions, associated with a greater root length, AM-colonization rate and root-secreted carboxylates per plant in domesticated crops. Domesticated crops displayed significantly greater plasticity (shown as response ratio) in AM colonization rate, but lower plasticity in specific root length, acid phosphatase activity, and amount of root-secreted carboxylates per plant than their wild relatives in response to low plant-available soil P concentrations. Crop domestication increased P-acquisition capacity by combining P-scavenging and P-mining strategies but largely restricted root plasticity, specifically in P-acquisition strategies. We confirmed trade-offs among P-scavenging strategies related to soil exploration and P-mining strategies related to root-secreted carboxylates. Our results highlight the domestication effects on crop P-acquisition strategies mediated by plant-soil interaction, providing insights to further improve crop P-acquisition efficiency.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101087"},"PeriodicalIF":3.4,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AMF and DSE diversity and colonization characteristics of Salvia miltiorrhiza in different genotypes and growth periods","authors":"Tingting Han ,&nbsp;Wanyun Li ,&nbsp;Ling Li ,&nbsp;Chao He ,&nbsp;Xianen Li","doi":"10.1016/j.rhisph.2025.101088","DOIUrl":"10.1016/j.rhisph.2025.101088","url":null,"abstract":"<div><div><em>Salvia miltiorrhiza</em> is a significant traditional Chinese medicine commonly utilized in the treatment of cardiovascular and cerebrovascular diseases. However, its application and development are hindered by issues related to mixed genotypes and the content of medicinal ingredients not meeting the standards. Arbuscular mycorrhizal fungi (AMF) and dark septate endophytes (DSE) are representative symbiotic fungi that show great potential for improving the growth and quality of <em>S. miltiorrhiza</em>. In this study, we examined the effects of genotype, growth period, and rhizosphere soil factors on species diversity, community composition, and colonization characteristics of AMF and DSE using morphological characteristics and molecular identification techniques. The results indicated that different genotypes and growth periods of <em>S. miltiorrhiza</em> could be colonized by AMF and DSE, although variations existed in the structure and sites of colonization. A total of 93 AMF strains from 11 genera and 35 DSE strains from 19 genera were isolated and identified. Among them, <em>Glomus</em> and <em>Acaulospora</em> were the dominant genera among AMF, while <em>Alternaria alternata</em> and <em>Macrophomina phaseolina</em> were common dominant species in DSE. Variance partitioning analysis indicated that the three-way interaction of genotype, growth period, and soil factors accounted for the highest percentages of AMF and DSE species diversity, at 58.06 % and 62.26 %, respectively. Two-factor analysis of variance indicated that both AMF and DSE species diversity exhibited a trend of October &gt; August &gt; March, with genotypes Z3 and Z4 demonstrating higher species diversity and segregation rates compared to Z1 and Z2. Correlation analysis shows that the main soil factors affecting the diversity of AMF and DSE include pH, SOC and NH₄⁺-N. In conclusion, this study not only enriched our understanding of the symbiotic relationship between <em>S. miltiorrhiza</em> and endophytic fungi (AMF and DSE), but also deepened our knowledge of their ecological functions. Furthermore, it provided a theoretical foundation for the isolation and conservation of endophytic fungal germplasm resources, as well as their subsequent application in the cultivation of Chinese medicinal materials.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101088"},"PeriodicalIF":3.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The AHL-driven cascade of rhizosphere microbes, enzymes, and nutrients contributes to the growth decline of Casuarina equisetifolia in continuous planting systems","authors":"Qingxu Zhang ,&nbsp;Yi Lin ,&nbsp;Lei Hong ,&nbsp;Yuhua Wang ,&nbsp;Miaoen Qiu ,&nbsp;Jianjuan Li ,&nbsp;Tingting Wang ,&nbsp;Zongnan Wu ,&nbsp;Mingmei Wu ,&nbsp;Wenxiong Lin ,&nbsp;Haibin Wang ,&nbsp;Zeyan Wu","doi":"10.1016/j.rhisph.2025.101086","DOIUrl":"10.1016/j.rhisph.2025.101086","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Acyl homoserine lactones (AHL), which are extensively studied quorum sensing (QS) signaling molecules in Gram-negative bacteria, play a significant role in regulating plant growth and shaping the rhizosphere ecosystem. In this study, we investigated the effects of continuous planting across multiple generations of the Australian pine &lt;em&gt;Casuarina equisetifolia&lt;/em&gt; (Casuarinaceae) on AHL accumulation in its rhizosphere soil. The study aims to identify key AHL and further employs an exogenous supplementation approach to assess their influence on the growth of &lt;em&gt;C. equisetifolia&lt;/em&gt; and the associated rhizosphere soil ecosystem. Our findings reveal a progressive increase in total AHL content, rising from 1.76 to 3.65 ng/g with continuous planting generations. The key AHL that significantly alter rhizosphere soil properties under continuous planting conditions are identified as C4-HSL, 3-oxo-C10-HSL, 3-oxo-C12-HSL, and 3-oxo-C14-HSL. Following exogenous treatment with C4-HSL, an increase in its concentration was correlated with a significant enhancement in both the root length and plant height of &lt;em&gt;C. equisetifolia&lt;/em&gt;. Conversely, treatments with 3-oxo-C10-HSL, 3-oxo-C12-HSL, and 3-oxo-C14-HSL resulted in a significant reduction in these growth parameters. While all four key AHL contributed positively to the proliferation of soil fungi and actinobacteria, their effects on bacterial populations exhibited variability. Following the exogenous application of the four key AHL, a significant reduction in the activities of urease and protease in the soil was observed. In contrast, the activities of acid phosphatase and cellulase were enhanced, leading to a decrease in the soil's available nitrogen and potassium content, while the available phosphorus content increased. Interaction effect analysis reveals that these key AHL collectively exert a strong positive regulatory effect on soil microbial abundance (0.979∗∗). Furthermore, soil microorganisms show a significant positive correlation with soil enzyme activity (0.997∗∗), whereas soil enzyme activity exhibits a strong negative correlation with the soil's available nutrient content (−0.995∗∗). Additionally, the soil's available nutrient content positively regulates the growth of &lt;em&gt;C. equisetifolia&lt;/em&gt; (0.970∗∗). The inhibitory effect of continuous planting on &lt;em&gt;C. equisetifolia&lt;/em&gt; growth primarily stems from reduced 3-oxo-C10-HSL levels coupled with elevated 3-oxo-C12-HSL and 3-oxo-C14-HSL concentrations in the rhizosphere. This alteration leads to a decrease in the bacterial population within the soil, which significantly reduces the activities of soil urease and protease, as well as the availability of nitrogen and potassium in the rhizosphere of &lt;em&gt;C. equisetifolia&lt;/em&gt;. Consequently, these changes result in markedly diminished root length and dry weight of &lt;em&gt;C. equisetifolia&lt;/em&gt;. This study provides a critical theoretical framework for the exogenous application of AHL to mo","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101086"},"PeriodicalIF":3.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global meta-analysis of endophytic inoculation effects on seed germination, seedling growth, and nutrient uptake of trees","authors":"Iflah Rafiq,&nbsp;Zafar A. Reshi","doi":"10.1016/j.rhisph.2025.101079","DOIUrl":"10.1016/j.rhisph.2025.101079","url":null,"abstract":"<div><div>Endophyte inoculation is emerging as a promising strategy to enhance tree growth, offering new possibilities for forest restoration in the face of climate change. While numerous studies report positive effects, a comprehensive quantitative synthesis remains lacking. This meta-analysis integrates findings from 71 studies, encompassing 1364 effect sizes, to evaluate the impact of endophyte inoculation on tree growth parameters across diverse experimental conditions, plant groups, and inoculum types. The results reveal significant positive effects on key growth variables, including root growth, shoot growth, height, dry weight, and nutrient uptake, with microbial consortia exerting the strongest influence. However, substantial heterogeneity in effect sizes was observed, shaped by differences in study design, environmental conditions, and host plant taxonomy. Angiosperms exhibited more pronounced growth responses than gymnosperms, while controlled environments, such as greenhouse conditions, yielded more consistent outcomes compared to field settings.</div><div>Variability in effect sizes, assessed through log response ratio (lnRR) and log coefficient of variation ratio (lnCVR) models, underscored the importance of both fixed and random effects in explaining observed trends. Notably, microbial consortia and bacterial inocula not only enhanced tree growth but also contributed to more stable and predictable outcomes, whereas fungal inocula exhibited greater variability in their effects. These findings highlight the potential of endophytes to promote tree growth and enhance stress resilience while emphasizing the need for standardized methodologies and ecological context considerations in future research. This work provides a comprehensive understanding of endophyte-mediated growth promotion and offers valuable insights for optimizing their application in forestry and ecosystem restoration.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101079"},"PeriodicalIF":3.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep tillage improves the rhizosphere environment in rainfed coffee cultivation on dense Cambisols through plant-soil interaction","authors":"Pedro Antônio Namorato Benevenute ,&nbsp;Fernandes Antônio Costa Pereira ,&nbsp;Samara Martins Barbosa ,&nbsp;Rodrigo Fonseca da Silva ,&nbsp;Maila Adriely Silva ,&nbsp;Gustavo Ferreira da Silva ,&nbsp;Jucelino de Souza Lima ,&nbsp;Leônidas Canuto Santos ,&nbsp;Flávio Henrique Silveira Rabêlo ,&nbsp;Aldir Carpes Marques Filho ,&nbsp;Geraldo César de Oliveira ,&nbsp;Bruno Montoani Silva","doi":"10.1016/j.rhisph.2025.101080","DOIUrl":"10.1016/j.rhisph.2025.101080","url":null,"abstract":"<div><div>Deep tillage alleviates drought stress in dense soils through soil-root interactions, serving as a key adaptation to climate change. Enhancing soil structure for root growth and water retention, supported by geostatistical techniques, is crucial for informed decision-making in sustainable agriculture. This study evaluated the effects of deep tillage methods and chemical amendments on soil properties and coffee plant performance in Nazareno, Minas Gerais, Brazil, on a clay-loam Cambisol. A randomized complete block design with split-plot arrangement was used, including three blocks, six depths (0–0.05, 0.15–0.20, 0.35–0.40, 0.55–0.60, 0.60–0.70, and 0.75–0.80 m), and five treatments: SP40 (furrower at 0.40 m), SP60 (Big Mix at 0.60 m, with liming as SP60AL), and SP80 (soil homogenizer at 0.60 m, subsoiler at 0.80 m, with liming as SP80AL). After five years, undisturbed soil samples were collected at different depths in the experimental area and an adjacent site under native Cerrado vegetation. In trenches, soil penetration resistance (PR), root growth, and chemical element contents were analyzed. Soil moisture was assessed using a probe from October 2019 to March 2021. ANOVA, Scott-Knott, and Dunnett tests (<em>p</em> &lt; 0.05) were used for data analysis. Geostatistical kriging mapped PR, root variables, element contents, and soil moisture. SP60 improved soil structure, root growth, and water uptake, enhancing antioxidant activity under drought. SP80AL enhanced water retention and root development up to 0.70 m but had lower drought tolerance. Geostatistical mapping was crucial for understanding deep tillage impacts on rhizosphere management, aiding sustainable coffee cultivation.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101080"},"PeriodicalIF":3.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased fine roots, exudates and altered rhizospheric functions in the invasive plant Sphagneticola trilobata compared to the native Sphagneticola calendulacea","authors":"Tongxi Tian ,&nbsp;Yina Yu ,&nbsp;Jingjing Yuan ,&nbsp;Jiaxin Sun ,&nbsp;Zhiyang Li ,&nbsp;Xiaomin Zhu ,&nbsp;Xiangcheng Wu ,&nbsp;Jun Wang ,&nbsp;Guangyan Ni","doi":"10.1016/j.rhisph.2025.101085","DOIUrl":"10.1016/j.rhisph.2025.101085","url":null,"abstract":"<div><div>The invasion of exotic plants has a significant impact on terrestrial ecosystems, while the underlying mechanisms are diverse and sometimes contradictory. A systematic study on root morphology and functions in the rhizosphere would shed light on a convincing invasion mechanism and practical control measures. We conducted a greenhouse experiment with the invasive <em>Sphagneticola trilobata</em> and the native <em>Sphagneticola calendulacea</em> to and systematically investigated their root morphology and rhizospheric functions. The growth, photosynthesis, root morphology and exudates, as well as the microorganisms in the rhizosphere were measured. The invasive <em>S. trilobata</em> not only grew faster and better, but also had a higher organic acid content in the root exudate than the native <em>S</em>. <em>calendulacea</em>, although their exudate chemical properties were similar. The invasive <em>S. trilobata</em> also had a greater amount of fine roots and a higher SRL than the native <em>S. calendulacea</em>, allowing for more root exudates to support the growth and reproduction of soil microbes, which in turn regulate the plant's nutrient uptake. We also found that <em>S. trilobata</em> alters the microbial composition of the soil, and influenced the β-diversity of fungi. These coordinated responses could promote the growth performance of <em>S. trilobata</em> and facilitate its invasion. Our results provide a framework for the mechanisms of plant invasion from the perspective of ecological processes in the subsurface rhizosphere.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101085"},"PeriodicalIF":3.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of arbuscular mycorrhizal hyphae on the structure of fungal and bacterial communities: An in situ study","authors":"Xianwang Du,&nbsp;Yicong Yin,&nbsp;Guowei Xia,&nbsp;Yuanshuang Yuan","doi":"10.1016/j.rhisph.2025.101082","DOIUrl":"10.1016/j.rhisph.2025.101082","url":null,"abstract":"<div><div>Soil microbial communities are key drivers of biogeochemical cycling in terrestrial ecosystems. However, under native soil conditions, since plant roots intimately interact with arbuscular mycorrhizal fungus (AMF) hyphae, the individual effects of AMF hyphae on the diversity and abundance of soil microbial communities remain unclear. Using an ingrowth core design, we isolated the hyphae of AMF from roots and quantified the affected soil microbial communities using meta-barcoding sequencing techniques. The results revealed that the presence of AMF hyphae significantly increased the diversity of fungi but not that of bacteria. In addition, AMF hyphae influenced the relative abundance of certain microbial taxa: the abundance of the phylum Ascomycota significantly increased, whereas that of the species <em>Mortierella horticola</em> and the phylum Mortierellomycota significantly decreased. The redundancy analysis results revealed that the soil nutrient availability and soil pH were closely related to the microbial community. In conclusion, AMF hyphae affect the structure of fungal and bacterial communities, and this process is regulated by soil properties. These findings provide new insight into the small-scale but complex interactions between the hyphae of AMF and microbes in terrestrial ecosystem soils from an <em>in situ</em> perspective.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101082"},"PeriodicalIF":3.4,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen addition and rhizosphere effects differentially shape prokaryotic and fungal communities in peanut–maize cropping systems","authors":"Yang Chen ,&nbsp;Zejin Zhang ,&nbsp;Yichao Rui ,&nbsp;Carson Pearl ,&nbsp;Sheng Lu ,&nbsp;Fang Wang ,&nbsp;Flemming Ekelund ,&nbsp;Yanbin Hao ,&nbsp;Xiaoyong Cui","doi":"10.1016/j.rhisph.2025.101084","DOIUrl":"10.1016/j.rhisph.2025.101084","url":null,"abstract":"<div><div>The role of nitrogen (N) fertilization in enhancing crop yields and mixed cropping in reducing continuous cropping obstacles have been well documented. However, N fertilization and mixed cropping on rhizosphere and bulk soil microbial diversity, community structure, and plant growth remain controversial. To address this knowledge gap, we conducted a pot experiment of two plant species (peanut (<em>Arachis hypogaea</em> L.) and maize (<em>Zea mays</em> L.)), three N addition levels (0, 150, and 225 kg N hm⁻²), and two cropping systems (monoculture and mixed cropping). To investigate rhizosphere and bulk soil prokaryotic and fungal community response to N addition in different cropping systems, high-throughput sequencing technology was used in this study. Overall, under the mixed cropping condition, compared with N₀ addition level, N₂ addition level increased maize aboveground biomass (AGB) by 205.62 % and maize belowground biomass (BGB) by 45.36 %, but reduced peanut AGB by 12.60 % and peanut BGB by 24.07 %. Moreover, with increased N fertilization, the BGB/AGB ratio of peanut under mixed cropping decreased first from 0.22 to 0.18, and then increased up to 0.23. The α-diversity of soil prokaryotic community decreased significantly with increasing N addition levels, while the observed ASVs of the rhizosphere fungal community was significantly lower than that of bulk soils. The results of non-metric multidimensional scaling (NMDS) combined with PERMANOVA analysis showed that the clustering of soil microbial communities was mainly dominated by the rhizosphere and N effects. In addition, Mantel test results showed that the changes of the rhizosphere environmental factors drove the changes in fungal community composition, while the changes of bulk soil environmental factors drove the changes in prokaryotic community composition. Microbial network co-occurrence analysis indicated that N addition increased the complexity of the soil prokaryotic network structure, but reduced the complexity of the soil fungal network structure. Meanwhile, the network structure complexity of prokaryotic and fungal communities in the rhizosphere were higher than that in bulk soils. The above results comprehensively suggested that N addition mainly changed soil prokaryotic community composition, while rhizosphere effects primarily altered soil fungal community composition. This may be caused by the survival strategy of soil microbes (r/K strategists) and the background environmental differences between the rhizosphere and bulk soils.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101084"},"PeriodicalIF":3.4,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143839189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transmission of bacterial community from seeds and rhizosphere to progeny in lima bean (Phaseolus lunatus)","authors":"Sandra Mara Barbosa Rocha ,&nbsp;Janderson Moura da Silva ,&nbsp;Romario Martins Costa ,&nbsp;Mayanna Karlla Lima Costa ,&nbsp;Regina Lucia Ferreira Gomes ,&nbsp;Ângela Celis de Almeida Lopes ,&nbsp;Ana Roberta Lima de Miranda ,&nbsp;Ademir Sérgio Ferreira Araujo","doi":"10.1016/j.rhisph.2025.101083","DOIUrl":"10.1016/j.rhisph.2025.101083","url":null,"abstract":"<div><div>The transmission of bacterial community from seeds and rhizosphere to progeny may have significant implications to next plant generations. Therefore, we evaluated the potential for transmission of bacterial taxa from seeds and rhizosphere to progeny and assessed the composition of the bacterial community in the soil, rhizosphere, and various plant compartments, including roots, leaves, and seeds. Seeds of lima bean (<em>Phaseolus lunatus</em>) were sown and during the flowering period, the soil, rhizosphere, roots, leaves, and seeds (progeny) were sampled. The V4 and V57 regions of 16S rRNA gene with region-specific primers were sequenced to soil/rhizosphere and plant, respectively. The composition of bacterial communities varied across soil and plant compartments at both the phylum and genus levels. Actinobacteria was abundant in soil and rhizosphere, while Proteobacteria was abundant in seeds and plant tissues. Genus-level differences included <em>Bacillus</em> in soil, rhizosphere, and roots, <em>Pseudomonas</em> in leaves, and <em>Acinetobacter</em> and <em>Pseudomonas</em> in seeds. Shared and exclusive taxa highlighted compartmental specificity in seeds and progeny. Thus, seeds and progeny exhibited 9 and 6 exclusive bacterial taxa, respectively, with <em>Acinetobacter</em>, <em>Pseudomonas</em>, and <em>Acidibacter</em> shared between them. This study identified distinct bacterial taxa across plant compartments and shows thar bacterial transmission to progeny highlights potential for next generations.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101083"},"PeriodicalIF":3.4,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Funneliformis mosseae exhibits greater improved effects on root hair development and phosphorus uptake in trifoliate orange than Claroideoglomus etunicatum","authors":"Chun-Yan Liu ,&nbsp;Xiao-Niu Guo ,&nbsp;Mashael Daghash Alqahtani ,&nbsp;Qiang-Sheng Wu","doi":"10.1016/j.rhisph.2025.101081","DOIUrl":"10.1016/j.rhisph.2025.101081","url":null,"abstract":"<div><div>Low-phosphorus (P) soils significantly limit agricultural productivity globally, particularly in regions where fertilizer application is restricted. Root hairs are crucial for the absorption of water and nutrients, while arbuscular mycorrhizal fungi (AMF) offer a promising approach to improve both root development and nutrient uptake. However, the molecular mechanisms by which specific AMF species affect root hair development in trifoliate orange (<em>Poncirus trifoliata</em>), a key citrus rootstock, are not well understood. In this study, the effects of <em>Funneliformis mosseae</em> (<em>F. mosseae</em>) and <em>Claroideoglomus etunicatum</em> (<em>C. etunicatum</em>) on root hair development, nutrient levels, and the expression of associated genes in trifoliate orange seedlings were studied. Both <em>F. mosseae</em> and <em>C</em>. <em>etunicatum</em> established symbiotic structures in the roots, but <em>F. mosseae</em> elicited a more pronounced response, as evidenced by enhanced plant growth, root development, root hair density, and length under <em>F. mosseae</em> versus <em>C. etunicatum</em>. Additionally, the two fungi increased phosphorus content and indole-3-acetic acid (IAA) levels, enhanced acid phosphatase activity, and reduced IAA oxidase activity, although <em>C. etunicatum</em> had a lesser effect on root IAA oxidase activity. The two fungal treatments upregulated the expression of <em>PtPAP1</em>, <em>PtaPT1</em>, and <em>PtaPT5</em>, while its down-regulated the expression of <em>PtPAP15</em>, <em>PtEXPA2</em>, and <em>PtEXPA4</em>. Correlation analysis revealed that <em>PtPAP1</em>, <em>PtaPT1</em>, and <em>PtaPT5</em> positively influenced root hair formation, while <em>PtPAP15</em>, <em>PtLAX5</em>, <em>PtABCB15</em>, and <em>PtPIN1</em> had negative effects on root hair formation. Overall, under low-phosphorus conditions, <em>F. mosseae</em> outperformed <em>C. etunicatum</em> in promoting root hair development and phosphorus uptake in trifoliate orange, driven by enhanced colonization, elevated IAA levels, and upregulated phosphate transporter genes. These findings highlight <em>F. mosseae</em>'s superior potential for optimizing root architecture and nutrient efficiency in citrus rootstocks.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101081"},"PeriodicalIF":3.4,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}