Rhizosphere最新文献

{"title":"Ecological and functional diversity of sulfur-oxidizing and sulfate-reducing bacteria in Chinese mangrove ecosystems: A comparative review with insights from Guangxi Beihai","authors":"Muhammad Kashif ,&nbsp;Qi Liang ,&nbsp;Can Meng ,&nbsp;Tingmei Li ,&nbsp;Yujia Luo ,&nbsp;Feng Guo ,&nbsp;Dan Wang ,&nbsp;Chengjian Jiang","doi":"10.1016/j.rhisph.2026.101260","DOIUrl":"10.1016/j.rhisph.2026.101260","url":null,"abstract":"<div><div>Mangrove habitats are critical coastal biomes at the land-sea interface, supporting various microbial communities necessary for biogeochemical cycling, including sulfur conversions. The principal players in these systems are sulfur-oxidizing bacteria (SOB) and sulfate-reducing bacteria (SRB), which accordingly control sulfur compounds oxidation and reduction. While SRB lowers sulfate to sulfide during organic material decomposition, SOB oxidizes harmful hydrogen sulfide (H₂S) to sulfate (SO₄²⁻). Using nutrient cycling, preservation of sulfur balance, and change of redox potential, these microbial interactions help preserve mangrove ecosystems' stability. Aiming toward Guangxi Beihai, Fujian, Guangdong, and Hainan, this review offers a comparative study of SOB and SRB populations in mangrove habitats throughout China. Salinity, organic matter content, redox potential, and anthropogenic influences affect regional microbial diversity and activity. Hainan's high salinity supports SOB, while Guangxi Beihai's prevailing SRB community has moderate salinity and high organic content. Reflecting their several environmental gradients, Fujian and Guangdong have a balanced presence in both groups. The functional roles of SOB and SRB in sulfur cycling, their interactions with carbon and nitrogen dynamics, and their contributions to ecosystem health are emphasized. It further explores the biotechnological potential of these bacteria in wastewater treatment, heavy metals detoxification, and large-scale environmental bioremediation. Notwithstanding developments in microbial ecology, significant knowledge gaps still exist about the adaptation mechanisms of SOB and SRB under environmental stressors, their interactions with other microbial taxa, and their reactions to climate change. Multi-omics techniques, including metagenomics and transcriptomics, should be used in future studies to disentangle sulfur-metabolizing bacteria's functional variety and ecological roles. Developing sustainable conservation plans, reducing anthropogenic effects, and increasing the resilience of mangrove ecosystems against global environmental change depend on an awareness of these microbial processes.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101260"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Divergent colonization strategies shape shared and inoculant-specific transcriptional and microbiome dynamics in pea roots","authors":"Ahmad H. Kabir","doi":"10.1016/j.rhisph.2025.101255","DOIUrl":"10.1016/j.rhisph.2025.101255","url":null,"abstract":"<div><div>Beneficial microbes can reprogram plant responses, but how ecologically distinct inoculants differentially shape host responses in pea remain unclear. To address this, we analyzed root transcriptional and microbiome responses to <em>Trichoderma afroharzianum</em> T22 and <em>Bacillus subtilis</em> NRRL B-14596. In RNA-seq analysis, <em>T</em>. <em>afroharzianum</em> induced a balanced set of 221 upregulated and 320 downregulated genes, whereas <em>B</em>. <em>subtilis</em> triggered a substantially broader response with 597 genes upregulated and 138 downregulated. <em>T. afroharzianum</em> established a robust endophytic presence within root tissues and induced a symbiosis-oriented transcriptional program, including <em>CASTOR/POLLUX, lectin-domain receptors</em>, <em>Nodule-specific GRP genes</em>, <em>Sucrose synthase</em> and <em>Glutamine synthetase</em>. This was further supported by co-culture assays, where <em>T. afroharzianum</em> significantly stimulated <em>Rhizobium leguminosarum</em> colony expansion, demonstrating a direct fungal–rhizobial synergism. In contrast, <em>B. subtilis</em> remained primarily epiphytic and triggered a broader, stress- and metabolism-focused transcriptome dominated by <em>Ethylene-responsive transcription factors</em>, <em>AUX/IAA repressors</em>, and metal-buffering and proton-gradient regulators, indicating hormone recalibration and rhizoplane homeostasis rather than Nod-factor signaling. Despite these divergent pathways, both microbes converged on antioxidant and detoxification responses, upregulating multiple antioxidant and glutathione-linked genes. Microbiome profiling further revealed that <em>T</em>. <em>afroharzianum</em> recruited symbiosis-enhancing bacterial and fungal taxa (Rhizobiales, <em>Pararhizobium</em>, Hypocreales), whereas <em>B</em>. <em>subtilis</em> selectively enriched a targeted guild of <em>Gemmatimonadales, Capnodiales, Flavobacterium, Paecilomyces known for</em> nutrient mobilization, auxin buffering, and stress mitigation. Together, these findings demonstrate that endophytic fungi and rhizoplane-associated bacteria reprogram host physiology through distinct yet complementary molecular routes, providing a blueprint for engineering synergistic fungal–bacterial consortia to enhance legume performance.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101255"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive shift to deep soil water use mitigates mining-induced drought stress in Artemisia ordosica","authors":"Xikai Wang ,&nbsp;Yunlan He ,&nbsp;Suping Peng ,&nbsp;Lei Chen","doi":"10.1016/j.rhisph.2026.101259","DOIUrl":"10.1016/j.rhisph.2026.101259","url":null,"abstract":"<div><div>In the ecologically fragile areas of western China, large-scale underground coal mining induces surface subsidence. These disturbances could alter plant water use strategy (WUS). However, the specific transformation path is not well understood. This study focused on <em>Artemisia ordosica</em> Krasch. (Asteraceae), comparing its WUS between subsidence and undisturbed area. By combining natural stable water isotopes (δ²H and δ¹⁸O) with deuterium labeling approach, we established a water source tracing pathway from soil to xylem. A Bayesian mixing model (MixSIAR) was applied to evaluate the effect of mining subsidence on plant WUS. The results indicated that: (1) Mining subsidence significantly altered near-surface water cycles, manifesting as depleted stable isotope values in both soil and xylem water in subsidence area (P &lt; 0.05); (2) <em>Artemisia ordosica</em> shifted its WUS in subsidence area, transitioning from primarily using shallow soil water (0–80 cm) in the undisturbed area to increasingly relying on deep soil water (80–120 cm). The proportion of shallow soil water uptake decreased by approximately 10.7 %, while deep soil water uptake increased by about 11.3 %; (3) Deuterium labeling effectively created distinct isotope gradients in soil profile, providing a reliable method for identifying plant water sources. This study demonstrates that <em>Artemisia ordosica</em> adapts to mining-induced environmental changes by actively adjusting its WUS.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101259"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linking root fungal endophytes to forest decline and management regimes in long-established Black pine stands","authors":"Luisa M. Manici ,&nbsp;Alessandro Paletto ,&nbsp;Francesco Caputo ,&nbsp;Claudia Becagli ,&nbsp;Isabella De Meo","doi":"10.1016/j.rhisph.2026.101278","DOIUrl":"10.1016/j.rhisph.2026.101278","url":null,"abstract":"<div><div>Non-mycorrhizal root endophytes often exist as harmless commensals but can turn pathogenic under host stress or aging. This study applies this behaviour to assess the health of a long-term <em>Pinus nigra</em> stand by analysing its fungal endophyte communities. A total of 744 fungal isolates were obtained from healthy feeder roots using culture-based methods in a pilot area of long-established black pine stands in southern Europe. They were identified on morphological traits and nucleotide sequencing in the ITS and histone-3 regions. The Dominance (64 %) of three potentially pathogenic species out of a total of 23 identified, i.e. <em>Dactylonectria torresensis</em>, <em>Diaporthe columnaris</em> and <em>Biscogniauxia mediterranea</em> indicated general declining tree health and compromised tree vigor.</div><div>There was no significant difference in fungal α-diversity between the two thinning techniques implemented eight years earlier, which had different impacts on wood biomass production and reforestation with native species. Conversely, significant changes in fungal species composition were observed primarily affecting the relative abundance of Diaporthe spp. and Cylindrocarpon-like fungi. Diaporthe spp. significantly increased under the innovative selective thinning which resulted in an improvement of biomass production as compared to traditional thinning techniques which increased Cylindrocarpon-like fungi. Conversely, <em>Xylariaceae (B</em>. <em>mediterranea)</em>, dark septate fungi (<em>Cadophora</em> sp., <em>Didymella aeria</em> and other 5 species), and mitosporic fungi (mainly <em>Penicillium</em>, <em>Trichoderma</em>, and <em>Fusarium</em> spp.) were unaffected by management and showed similar frequencies across treatments.</div><div>Overall, non-mycorrhizal root endophytes proved to be sensitive ecological indicators of forest health and management-driven changes offering valuable insights into the mechanisms that support pine forest health.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101278"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Threshold-dependent metabolic and microbiome reprogramming triggered by mechanical root injury in Populus","authors":"Jinxiu Cai ,&nbsp;Ying Liu ,&nbsp;Shuwen Yang ,&nbsp;Yong Guo ,&nbsp;Ziyang Zhu","doi":"10.1016/j.rhisph.2026.101295","DOIUrl":"10.1016/j.rhisph.2026.101295","url":null,"abstract":"<div><div>Mechanical injury is a widespread and ecologically relevant stress experienced by woody plants, yet the capacity of the root–microbiome interface to buffer such disturbance through metabolic regulation remains poorly quantified. Here, we conducted an in situ manipulative experiment with a root-severance gradient (0, 25%, 50%, 75%, and 100%; <span><math><mrow><mi>n</mi><mo>=</mo><mn>3</mn></mrow></math></span> biological replicates per level) on Populus trees in a semi-arid field environment to investigate the coupled responses of rhizosphere chemistry (rhizosphere soil metabolome) and the rhizosphere microbiome. After a 20-day post-severance equilibration period, we characterized rhizosphere metabolite profiles (untargeted LC–MS) and bacterial communities (16S rRNA sequencing) concurrently. Multi-omics integration revealed a non-linear trajectory in rhizosphere reorganization: metabolite composition exhibited a distinct threshold-like transition at approximately 75% root severance, shifting from growth-oriented metabolism to a defense-dominant state (e.g., accumulation of phenolics and isoflavonoids), thereby reshaping rhizosphere chemical niches. This metabolic reprogramming coincided with a synchronous reassembly of the bacterial community (Procrustes <span><math><msup><mrow><mi>m</mi></mrow><mrow><mn>2</mn></mrow></msup></math></span> = 0.63, <span><math><mrow><mi>P</mi><mo>=</mo><mn>0</mn><mo>.</mo><mn>006</mn></mrow></math></span>), characterized by a stepwise turnover from copiotrophs to stress-tolerant taxa, with a concomitant shift in dominant phyla (e.g., Acidobacteriota). Correlation network analysis further supported a “defense–recruitment” pattern, in which defense-associated specialized metabolites (e.g., terpenoid lactones and phenolics) emerged as topological hubs in co-occurrence networks, coinciding with selective enrichment of stress-tolerant bacterial taxa. Collectively, these findings uncover a threshold-controlled regulatory mode by which woody plant roots coordinate rhizosphere metabolic reprogramming and microbiome assembly in response to mechanical injury, highlighting a critical boundary at which rhizosphere chemical niches and microbial assembly shift states. These insights advance mechanistic understanding of root injury responses and provide a physiological basis for hypothesis-driven manipulation of rhizosphere processes in physically disturbed habitats.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101295"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147395935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The key predictive factors of arbuscular mycorrhizal fungi community spatial distribution in saline-alkaline meadow soils: Abiotic variables or hosts?","authors":"Lin Zhang ,&nbsp;Yuqiang Wen ,&nbsp;Mengmeng Zhang ,&nbsp;Kun Li ,&nbsp;Ruotong Wu ,&nbsp;Ning Sun ,&nbsp;Fuqiang Song ,&nbsp;Tianle Xu","doi":"10.1016/j.rhisph.2026.101266","DOIUrl":"10.1016/j.rhisph.2026.101266","url":null,"abstract":"<div><div>Arbuscular mycorrhizal (AM) fungi are vital plant symbionts in terrestrial ecosystems, yet their community distribution patterns and key drivers in extreme environments remain elusive. Focusing on the unique soda saline-alkaline meadows in the cold region of Northeast China, we established six sampling sites along a 500-km climatic gradient. By integrating traditional microscopic techniques with high-throughput sequencing, we systematically characterized the spatial distribution and drivers of soil AM fungal communities under severe saline-alkaline stress. Results indicated that under saline-alkaline stress, abiotic variables overrode host preferences to dominate community structuring. Specifically, high pH significantly suppressed root colonization and extraradical mycelial density, and significantly reduced soil AM fungal Shannon diversity. Nitrate nitrogen (NO₃⁻-N) acted as a critical \"diversity filter,\" showing a significant negative correlation with species richness and phylogenetic diversity. Conversely, soil organic carbon (SOC), total nitrogen (TN), and regional climate synergistically drove community composition divergence. Crucially, the lack of significant correlation between soil AM fungal and plant communities confirmed the primacy of abiotic filtering in these extreme habitats. Further analysis revealed divergent ecological strategies: Regional climate potentially regulated the abundance of the dominant genera <em>Glomus</em> and <em>Scutellospora</em>, whereas <em>Rhizophagus</em> was driven by local nutrient-rich micro-niches (SOC and ammonium nitrogen) and closely associated with plant productivity. This study identifies severe soil abiotic stress as the core driver shaping AM fungal spatial patterns in cold saline-alkaline meadows. These findings deepen our understanding of microbial distribution in stressed habitats and offer critical scientific insights for the restoration of fragile saline-alkaline ecosystems.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101266"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"25 years of cold storage decreases protein concentration but preserves other metabolite pools in spores of an arbuscular mycorrhizal fungus","authors":"Eduarda Lins Falcão ,&nbsp;Mohamed Hijri ,&nbsp;Fábio Sérgio Barbosa da Silva","doi":"10.1016/j.rhisph.2026.101272","DOIUrl":"10.1016/j.rhisph.2026.101272","url":null,"abstract":"<div><div>Long-term cold storage of arbuscular mycorrhizal fungal (AMF) inocula can reduce spore infectivity and viability, yet the underlying biochemical causes remain poorly understood. We tested whether extended storage duration affects the metabolic profile of <em>Entrophospora etunicata</em> spores. Spores originating from an inoculum produced in 2000 (EE2000), using sand and vermiculite as substrate and millet as host, and stored under cold conditions for 25 years were compared with spores from an inoculum produced in 2023 under identical conditions and from the same starter culture (EE2023). Metabolite extraction from 200 spores was performed to spectrophotometrically quantify total triglycerides, proteins, flavonoids, soluble carbohydrates, phenolics, and antioxidant capacity. Means were compared using two-sample <em>t</em>-test (95% confidence). Protein concentration was 28% lower in EE2000 spores relative to EE2023 (<em>p</em>≤ 0.05), whereas triglycerides, flavonoids and antioxidant activity did not differ between storage periods. Total phenolics and soluble carbohydrates were below detection limits. The decline in protein content suggests storage-derived oxidative effect that may contribute to the reduced colonization potential and viability previously reported for long-stored AMF inocula. We conclude that more than two decades of cold storage negatively affect the protein metabolism in <em>E. etunicata</em> spores. This study provides the first evidence of negative effect of prolonged storage on protein content in AMF spores.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101272"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The regulatory role of different exudates on rhizosheath in Kengyilia hirsuta seedlings","authors":"Chen Chen ,&nbsp;Fang Liu ,&nbsp;Yuanhang Wang ,&nbsp;Siqi Yuan ,&nbsp;Yue Jia ,&nbsp;Hao Guan ,&nbsp;Hui Wang ,&nbsp;Bo Wei ,&nbsp;Youjun Chen","doi":"10.1016/j.rhisph.2026.101281","DOIUrl":"10.1016/j.rhisph.2026.101281","url":null,"abstract":"<div><div>As an alpine plant species native to the Qinghai-Tibet Plateau, <em>Kengyilia hirsuta</em> plays a crucial ecological role in maintaining the structural and functional stability of the ecosystem. Its specialized rhizosheath structure markedly improves the efficiency of water and nutrient uptake, thereby conferring enhanced drought tolerance and resilience in nutrient-deficient environments. Through metabolomic analysis, three differentially accumulated metabolites (arabinose, erythrose, and maltotriose) and soybean lecithin were identified as exogenous additives to evaluate their regulatory effects on root development in <em>K. hirsuta</em>. This study reveals that erythrose, maltotriose, and soybean lecithin induced an 18–23 % reduction in xylem vessel diameter, while the cortex-stele ratio remained stable. Notably, maltotriose treatment increased the total length of root by 34 % and elevated phytohormone levels (indole-3-acetic acid +27 %; cytokinins +19 %), yet exhibited no stimulatory effect on root hair proliferation. Intriguingly, all treatments resulted in thicker endodermal Casparian strips (1.4-2.1 × controls), suggesting reinforced ion selective filtration. Periodate-Schiff staining unveiled unique granular polysaccharide deposition patterns in root cell walls, indicative of specialized carbohydrate metabolism. Crucially, maltotriose treatment increased rhizosheath biomass by 41 % and soil adhesion capacity by 29 %, directly linking carbohydrate exudation to rhizosheath-mediated environmental adaptation. Our results demonstrate that specific sugars and phospholipids act as metabolic switches coordinating xylem remodeling and soil binding, with maltotriose emerging as a central regulator of <em>K. hirsuta</em>'s root architectural plasticity and ecological competitiveness in desertified grasslands of the Qinghai-Tibet Plateau.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101281"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil rejuvenation influences crop specific selective enrichment of rhizospheric microbiome","authors":"Prabhakar D. Pandit ,&nbsp;Rajesh Pal ,&nbsp;Rahul Warke ,&nbsp;Hemant J. Purohit ,&nbsp;Gangadhar M. Warke","doi":"10.1016/j.rhisph.2026.101285","DOIUrl":"10.1016/j.rhisph.2026.101285","url":null,"abstract":"<div><div>The prominence of organic inputs is increased in climate-smart, sustainable agricultural systems to address soil deterioration as an alternative to inorganic and chemical inputs. This study analyses the effect of the application of soil rejuvenator HPP-P3 (SR-HPP-P3), a biostimulant on soil rhizospheric microbiome in soybean, cotton, pigeon pea, and turmeric crops at field level. We hypothesize that application of SR-HPP-P3 influences a crop-specific reorganization of the rhizospheric microbiome through improved functional activity leading to increase in crop yield. By drenching, the three doses of SR-HPP-P3 were applied after sowing of all four crops, with only the recommended dose of fertilizers (RDF), and without SR-HPP-P3 application as the control. The trial showed an increase in yield in all crops which was associated with application of SR-HPP-P3. The yield increase of 57 %, 39 %, 21 % and 20 % was observed in case of soybean, cotton, pigeon pea and turmeric crop respectively as compared with control. Soil metagenome DNA sequence analysis demonstrated the crop-specific rhizospheric microbiome, which was coincided with enhanced plant growth promoting rhizobacteria (PGPR) after drenching treatment. We supported the observations of metagenome analysis with the rhizospheric soil's physiological functional analysis for nutrient availability. 18 % increase in nitrogen fixing colony-Forming units CFU count was observed in case of cotton crop soil. The highest P and K solubilization index (3.3 and 2.6 respectively) was observed in soybean crop soil, whereas highest IAA production (27.2 μg/g soil) was also associated with soybean crop soil. Thus enhanced crop yield was correlated with rhizospheric bacterial community reshaping, with an increase in bacterial species richness. Thus the preliminary study proposes that soil rejuvenation through SR-HPP-P3 facilitates richness in the microbial community with an increase in crop yield, wherein metagenome analysis data suggest it's due to crop-specific enrichment of rhizospheric community.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101285"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant species and altitudinal gradients jointly shape rhizosphere bacterial community structure in mountain ecosystems","authors":"Yixin Song ,&nbsp;Tian Zhang ,&nbsp;Maryamgul Yasen ,&nbsp;Mingyuan Li ,&nbsp;Jilian Wang","doi":"10.1016/j.rhisph.2025.101245","DOIUrl":"10.1016/j.rhisph.2025.101245","url":null,"abstract":"<div><div>In mountainous systems, elevation gradients regulate soil properties and plant distribution. Rhizosphere bacteria are key mediators of soil-plant interactions, and their altitudinal variation and host association are core to deciphering the adaptation mechanisms of mountainous ecosystems. In our study, 16S amplicon sequencing was employed to analyze the rhizosphere soil bacterial community structures of different plant species along altitude gradients (1500–4000 m) on the eastern Pamir Plateau, China. The three plant species include <em>Cirsium japonicum</em>, <em>Phragmites australis</em>, and <em>Medicago sativa</em>. The results showed the richness and diversity of bacteria ranked in the order <em>M</em>. <em>sativa</em>, <em>P</em>. <em>australis</em>, <em>and C</em>. <em>japonicum</em>, and the response to altitude varied among plant species. Proteobacteria (34.2 %–41.5 %) and Actinobacteriota (22.8 %–28.6 %) were the dominant phyla, and the bacterial communities were sensitive to altitude changes. The functions of bacteria inferred from 16S data were dominated by metabolism (45.5 %–49.8 %) and genetic information processing (20.4 %–22.3 %), and also exhibited plant-altitude-specific differentiation. Only the bacterial diversity of <em>C</em>. <em>japonicum</em> and <em>P</em>. <em>australis</em> was correlated with soil nutrients, while no significant correlation was observed for <em>M</em>. <em>sativa</em>. Moreover, the correlation between bacterial functions and the rhizosphere bacteriota, as well as soil nutrients, varied among plant species. The metabolic functions were enhanced at 3500 m and 4000 m, and the genetic information processing function of <em>M</em>. <em>sativa</em> was prominent. The partial least squares path model further confirmed the unique altitude adaptation strategies of the three plant species. This study provides data support for deciphering the altitudinal adaptation mechanisms of alpine plant-microbe interactions.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101245"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}