Rhizosphere最新文献_第2页

Key processes of iron acquisition in strategy I plants: Assessment by mechanistic modelling 战略I工厂铁获取的关键过程：机械模型评估

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2026-01-23 DOI: 10.1016/j.rhisph.2026.101279

Thibault Sterckeman

Key mechanisms of iron uptake as highlighted by recent modelling works are reviewed. Calculations indicate that Fe²⁺ is often much more abundant than Fe³⁺ in soil solutions, contrarily to what is generally considered in the literature. However, mechanistic modelling of root Fe uptake shows that the concentrations of these ions are far below the levels required by plants. Furthermore, the acidification hypothetically triggered by iron deficiency is insufficient to increase their levels to the required extent. This confirms that AHA2, FRO2 and IRT1 evolved not only to process free ferric and ferrous ions, but also Fe-complexes with organic ligands. Numerical simulations show that aqueous dissociation of those Fe-complexes close to the root cell membrane is never quick enough to supply the root. Therefore, Fe complexes may dissociate at high speed after forming a transient ternary complex with a biotic ligand at the cell membrane near the AHA2-FRO2-IRT1 system. This catalyses Fe³⁺ reduction and internalisation of Fe²⁺. Moreover, a Fe ligand exuded by roots would be efficient only if it dissolves the soil iron hydroxides through a surface dissolution process much quicker than the aqueous dissolution. Simulations show that the mean concentration of Fe complexed by microbial siderophores in soils is sufficient to meet plant needs for iron, considering a moderate ‘shaping’ of the rhizosphere microflora and supposing that the roots are able to process the various types of complexes. Simulations also show that root absorption of intact Fe-complexes is as efficient as their processing by AHA2-FRO2-IRT1.

铁摄取的关键机制，强调了最近的建模工作进行了审查。计算表明，在土壤溶液中，Fe2+往往比Fe3+丰富得多，这与文献中普遍认为的相反。然而，根系铁吸收的机制模型表明，这些离子的浓度远远低于植物所需的水平。此外，假设由缺铁引发的酸化不足以将它们的水平提高到所需的程度。这证实了AHA2， FRO2和IRT1不仅可以处理游离铁离子和亚铁离子，还可以处理与有机配体的铁配合物。数值模拟表明，靠近根细胞膜的铁络合物的水解离速度永远不足以供应根。因此，Fe配合物在靠近AHA2-FRO2-IRT1体系的细胞膜上与生物配体形成瞬态三元配合物后可能会高速解离。这催化了Fe3+的还原和Fe2+的内化。此外，根渗出的铁配体只有在通过比水溶快得多的表面溶解过程溶解土壤铁氢氧化物时才有效。模拟表明，考虑到根际微生物群的适度“塑造”，并假设根能够处理各种类型的复合物，土壤中微生物铁载体络合的平均铁浓度足以满足植物对铁的需求。模拟还表明，完整铁配合物的根吸收效率与AHA2-FRO2-IRT1处理效率相当。

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引用次数: 0

Wisteria sinensis and Lonicera japonica exhibit distinct cadmium tolerance mechanisms and potential for ecological restoration of cadmium-contaminated soils 紫藤和忍冬表现出不同的耐镉机制和镉污染土壤的生态修复潜力

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2026-01-22 DOI: 10.1016/j.rhisph.2026.101277

Wenxi Guan , Zhengze Chen , Jiarui Wang , Yanan Liu , Dazhuang Huang , Xiaoyun Niu

Phytoremediation is an eco-friendly and cost-effective method for remediating soils contaminated by heavy metals. However, research on the tolerance of woody lianas to cadmium (Cd) remains relatively limited. Therefore, this study selected six woody lianas—Wisteria sinensis, Parthenocissus tricuspidata, Campsis grandiflora, Periploca sepium, Lonicera japonica, and Ampelopsis humulifolia—and conducted pot experiments with Cd concentrations (0.78, 11.51, and 28.51 mg kg⁻¹) to evaluate their physiological responses and tolerance to Cd stress. Results revealed significant variations in Cd stress tolerance among the species. Functional analysis indicated that W. sinensis displayed the highest overall resistance under elevated concentrations of Cd stress. The underlying mechanisms include: (1) sequestering 40 %∼60 % of Cd²⁺ in the cell wall; (2) the antioxidant enzyme system is markedly activated, with the activities of POD, CAT, and SOD increasing by 125.00 %, 325.00 %, and 57.79 %, respectively, to mitigate oxidative damage; (3) maintaining stable photosynthesis alongside a sharp increase in root nitrogen. Conversely, L. japonica adapted well to low Cd stress by enhancing soluble sugar content and shoot transport. Nonetheless, the accumulation coefficients of all the tested plants are relatively low. Specifically, for W. sinensis, these coefficients are 0.64 and 0.44 under low and high Cd treatments, respectively, indicating a considerable distance from hyperaccumulator status. In conclusion, while lacking strong accumulation, these lianas display distinct physiological adaptability. Their climbing growth and ornamental value make them suitable for ecological restoration and vertical greening in Cd-contaminated areas such as mines and urban outskirts.

植物修复是一种生态友好、经济有效的重金属污染土壤修复方法。然而，木质藤本植物对镉（Cd）耐受性的研究相对有限。因此，本研究选择紫藤、孤雌藤、大花Campsis granflora、金银花Periploca sepium、忍冬忍冬Ampelopsis humulifolia 6种木本藤本植物，在Cd浓度（0.78、11.51和28.51 mg kg−1）下进行盆栽试验，评价它们对Cd胁迫的生理反应和耐受性。结果表明，不同品种间镉胁迫耐受性存在显著差异。功能分析表明，在高浓度Cd胁迫条件下，中华水杨表现出最高的综合抗性。潜在的机制包括：(1)在细胞壁中隔离40 % ~ 60 %的Cd2+；(2)抗氧化酶系统被显著激活，POD、CAT和SOD活性分别提高了125.00 %、325.00 %和57.79 %，减轻了氧化损伤；(3)在根系氮急剧增加的同时，维持稳定的光合作用。相反，粳稻通过提高可溶性糖含量和茎部运输，较好地适应了低镉胁迫。然而，所有被试植株的累积系数都相对较低。在低Cd和高Cd处理下，这些系数分别为0.64和0.44，表明与超积累体状态有相当大的距离。综上所述，这些藤本植物虽然缺乏强烈的积累，但具有明显的生理适应性。攀缘生长和观赏价值使其适合于矿区、城郊等镉污染地区的生态修复和垂直绿化。

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引用次数: 0

Linking root fungal endophytes to forest decline and management regimes in long-established Black pine stands 在历史悠久的黑松林分中，根真菌内生菌与森林衰退和管理制度的联系

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2026-01-20 DOI: 10.1016/j.rhisph.2026.101278

Luisa M. Manici , Alessandro Paletto , Francesco Caputo , Claudia Becagli , Isabella De Meo

Non-mycorrhizal root endophytes often exist as harmless commensals but can turn pathogenic under host stress or aging. This study applies this behaviour to assess the health of a long-term Pinus nigra stand by analysing its fungal endophyte communities. A total of 744 fungal isolates were obtained from healthy feeder roots using culture-based methods in a pilot area of long-established black pine stands in southern Europe. They were identified on morphological traits and nucleotide sequencing in the ITS and histone-3 regions. The Dominance (64 %) of three potentially pathogenic species out of a total of 23 identified, i.e. Dactylonectria torresensis, Diaporthe columnaris and Biscogniauxia mediterranea indicated general declining tree health and compromised tree vigor.

There was no significant difference in fungal α-diversity between the two thinning techniques implemented eight years earlier, which had different impacts on wood biomass production and reforestation with native species. Conversely, significant changes in fungal species composition were observed primarily affecting the relative abundance of Diaporthe spp. and Cylindrocarpon-like fungi. Diaporthe spp. significantly increased under the innovative selective thinning which resulted in an improvement of biomass production as compared to traditional thinning techniques which increased Cylindrocarpon-like fungi. Conversely, Xylariaceae (B. mediterranea), dark septate fungi (Cadophora sp., Didymella aeria and other 5 species), and mitosporic fungi (mainly Penicillium, Trichoderma, and Fusarium spp.) were unaffected by management and showed similar frequencies across treatments.

Overall, non-mycorrhizal root endophytes proved to be sensitive ecological indicators of forest health and management-driven changes offering valuable insights into the mechanisms that support pine forest health.

非菌根根内生菌通常作为无害的共生体存在，但在寄主胁迫或老化下可能变成致病性的。本研究通过分析其真菌内生菌群落，应用这种行为来评估长期黑松林分的健康状况。在南欧一个历史悠久的黑松林试验区，采用基于培养的方法从健康的取食根中获得了总共744株真菌分离株。通过ITS和组蛋白-3区域的形态特征和核苷酸测序对它们进行了鉴定。在鉴定的23种潜在致病性树种中，3种（Dactylonectria torresensis、Diaporthe columnaris和Biscogniauxia mediterrania）的优势度（64% %）表明树木健康状况普遍下降，树木活力受损。8年前两种间伐方法的真菌α-多样性差异不显著，这对木材生物量生产和本地种造林有不同的影响。相反，真菌种类组成的显著变化主要影响了Diaporthe spp.和圆柱类真菌的相对丰度。与传统的疏林技术相比，创新的选择性疏林技术显著增加了Diaporthe spp.的生物量，从而提高了类柱碳类真菌的产量。相反，木木科（地中海木木科）、暗隔真菌（Cadophora sp.、Didymella aeria等5种）和有丝孢子真菌（主要是青霉、木霉和镰刀菌）不受管理影响，在不同处理中呈现相似的频率。总体而言，非菌根内生菌被证明是森林健康和管理驱动变化的敏感生态指标，为支持松林健康的机制提供了有价值的见解。

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引用次数: 0

An overlooked synergy: arbuscular mycorrhizal fungi and increased accumulation of plant saponins 一个被忽视的协同作用：丛枝菌根真菌和植物皂苷积累增加

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2026-01-17 DOI: 10.1016/j.rhisph.2026.101274

Eduarda Lins Falcão , João Gabriel Lira de Carvalho , Jackson Roberto Guedes da Silva Almeida , Qiang-Sheng Wu , Fábio Sérgio Barbosa da Silva

The use of arbuscular mycorrhizal fungi (AMF) has been recognized as an effective strategy to increase the accumulation of plant secondary metabolites. However, the role of this approach in promoting saponin production, molecules with broad applications across various industrial sectors, has received limited attention. Thus, this opinion paper aimed to synthesize studies that have investigated AMF inoculation to improve saponin accumulation. Thirty-five relevant publications on this topic were selected and their key findings were highlighted, such as the most frequently studied plant and AMF genera, and whether bioactivities were evaluated. The results underscore the potential of AMF in saponin biosynthesis, while also identifying research gaps that need to be addressed to enable large-scale application of this technology.

利用丛枝菌根真菌（AMF）已被认为是增加植物次生代谢产物积累的有效策略。然而，这种方法在促进皂素生产中的作用，在各个工业部门具有广泛应用的分子，受到的关注有限。因此，本文旨在综合有关接种AMF促进皂苷积累的研究。本文选取了35篇与该主题相关的出版物，并重点介绍了它们的主要发现，如最常被研究的植物和AMF属，以及是否对生物活性进行了评估。这些结果强调了AMF在皂素生物合成中的潜力，同时也确定了需要解决的研究空白，以使该技术能够大规模应用。

引用次数: 0

Comparative taxonomic and functional profiling of rhizosphere and root endophytic bacterial communities in the mangrove Rhizophora apiculata 红树根际和根内生细菌群落的比较分类和功能分析

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2026-01-17 DOI: 10.1016/j.rhisph.2026.101276

K.A. Wafha , K. Devika Raj , Anas Abdulaziz , Baby Divya

The diverse bacterial community present in various compartments of the mangrove, including, rhizosphere, rhizoplane, phyllosphere and endosphere, reflects a vital ecological function necessary for thriving in conditions characterised by high salinity, tidal fluctuations, and waterlogging. This study employed 16S rRNA gene amplicon sequencing (metabarcoding) using the Illumina high-throughput sequencing technology for the comprehensive analysis of bacterial diversity and its ecosystem functioning within the rhizosphere compartment (RC) and root endophytic compartment (EC) of the salt-tolerant mangrove species Rhizophora apiculata. This study revealed significantly higher bacterial diversity in the RC compared to that found in the EC, as assessed by both α-diversity and β-diversity metrices. The taxonomic analysis identified a total of 58 bacterial phyla, belonging to 163 classes, 419 orders, 672 families, 1187 genera and 2559 species in both the compartments. The five predominant bacterial phyla identified were Proteobacteria, Acidobacteriota, Bacteroidota, Patescibacteria and Desulfobacteriota. Among these phyla, Proteobacteria were the most abundant in both RC and EC, followed by Desulfobacteriota in the EC and Acidobacteriota in the RC. Furthermore, a core set of abundant bacterial genera was observed in both the endophytic and rhizospheric communities. These genera hitherto play essential roles in nutrient cycling, pollutant degradation and the maintenance of ecosystem stability. The dominant genera were Sulfurifustis, Spirochaeta, Novosphingobium, Candidatus Moranbacteria, Lacunisphaera, Candidatus Kaiserbacteria, Ignavibacterium, Dongia and Candidatus sp. Computational predictions indicated that the metabolic versatility associated with the functional genes included cellular processes, nutrient metabolism and genetic information processing. Endosphere associated bacteria are linked to s adaptive microbial strategies whereas rhizosphere communities drive organic matter turn over, redox regulation and environmental buffering. This study explores the complexassociation between Rhizophora apiculata and its associated bacteriome and offers valuable insights into this relationship.

红树林的不同区域（包括根际、根面、根层和内层）中存在着不同的细菌群落，这反映了在高盐度、潮汐波动和涝渍条件下繁茂生长所必需的重要生态功能。本研究利用Illumina高通量测序技术，采用16S rRNA基因扩增子测序（metabarcoding）技术，对耐盐红树根际室（Rhizophora apiculata）和根内生室（Rhizophora apiculata）细菌多样性及其生态系统功能进行综合分析。本研究显示，通过α-多样性和β-多样性指标评估，RC中的细菌多样性明显高于EC。经分类分析，两个区室共有58门细菌，隶属于163纲，419目，672科，1187属，2559种。鉴定出的5个优势菌门为变形菌门、酸性菌门、拟杆菌门、Patescibacteria和Desulfobacteriota。在这些门中，变形菌门在RC和EC中都是最多的，其次是EC中的Desulfobacteriota和RC中的酸性菌门。此外，在内生和根际群落中都观察到一组丰富的核心细菌属。迄今为止，这些属在养分循环、污染物降解和维持生态系统稳定方面发挥着重要作用。优势属为硫霉属、螺旋体、Novosphingobium、Candidatus Moranbacteria、Lacunisphaera、Candidatus Kaiserbacteria、Ignavibacterium、Dongia和Candidatus sp.。计算预测表明，与功能基因相关的代谢多样性包括细胞过程、营养代谢和遗传信息处理。内圈相关细菌与5种适应性微生物策略有关，而根际群落驱动有机质周转、氧化还原调节和环境缓冲。本研究探讨了尖根霉及其相关菌群之间的复杂关系，并为这种关系提供了有价值的见解。

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引用次数: 0

Continuous cropping obstacle in Ganoderma lucidum is driven by antagonistic bacterial enrichment and soil bacterial community imbalance 灵芝连作障碍是由拮抗菌富集和土壤细菌群落失衡驱动的

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2026-01-17 DOI: 10.1016/j.rhisph.2026.101267

Xiaomeng Lv, Xiaofang He, Yuting Zhuang, Jinxiang Zhang, Jianhui Chen, Ying Zhou, Senxiang Xie, Lixin Liang, Liping Deng, Xiaoping Wu

Ganoderma lucidum is a valuable medicinal mushroom used extensively in traditional medicine and modern health industries. Continuous cropping often alters soil microbial communities, leading to continuous cropping obstacles that limit sustainable production. Although fungal diversity has been extensively studied, bacterial dynamics and their functional roles remain less understood. Here, we combined bacterial isolation and identification, third-generation full-length 16S rDNA amplicon sequencing, and confrontation assays to systematically investigate soil bacterial communities before and after G. lucidum cultivation. Significant changes in microbial richness, diversity, and composition were observed with notable shifts in Proteobacteria, Firmicutes, and Acidobacteria. Importantly, we identified key bacterial species, including Bacillus velezensis, B. subtilis, B. amyloliquefaciens, Streptoverticillium reticulum, and Paenibacillus mucilaginosus, that strongly inhibited G. lucidum mycelial growth, highlighting their potential role in continuous cropping obstacles. This study provides the first integrative framework linking species-level bacterial dynamics to continuous cropping barriers in G. lucidum, offering a theoretical basis for targeted microbial management in sustainable cultivation.

灵芝是一种被广泛应用于传统医药和现代保健产业的珍贵药用蘑菇。连作经常改变土壤微生物群落，导致连作障碍，限制可持续生产。尽管真菌多样性已被广泛研究，但细菌动力学及其功能作用仍然知之甚少。本研究采用细菌分离鉴定、第三代16S rDNA全长扩增子测序和对抗试验相结合的方法，系统研究了绿植前后土壤细菌群落。微生物丰富度、多样性和组成发生了显著变化，变形菌门、厚壁菌门和酸性菌门发生了显著变化。重要的是，我们发现了一些关键的细菌物种，包括velezensis芽孢杆菌、枯草芽孢杆菌、解淀粉芽孢杆菌、网状链杆菌和粘液芽孢杆菌，它们强烈地抑制了G. lucidum菌丝体的生长，突出了它们在连作障碍中的潜在作用。本研究提供了第一个将种属水平的细菌动态与绿豆连作障碍联系起来的综合框架，为可持续栽培中有针对性的微生物管理提供了理论依据。

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引用次数: 0

Plant coexistence shapes microbial carbon and phosphorus limitations in soils of expanding alpine shrubs 植物共存决定了扩张型高山灌木土壤微生物碳磷限制

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2026-01-16 DOI: 10.1016/j.rhisph.2026.101275

Zhiliang Ma, Yamei Chen, Wenjuan Xu

Alpine shrub expansion restructures plant communities and soil biogeochemistry on the Qinghai-Tibetan Plateau, but how contrasting plant coexistence patterns (shrub-conifer vs. mixed shrubs) shape microbial metabolic limitation across bulk/rhizosphere soils and soil layers remains unresolved—limiting predictions of ecosystem responses to vegetation shifts. We quantified microbial metabolic limitation via extracellular enzyme stoichiometry and vector properties in bulk/rhizosphere soils (organic/mineral layers) of expanding Salix oritrepha shrubs under three coexistence patterns: pure stands, coexistence with Picea likiangensis (shrub-conifer), or Sibiraea angustata (mixed shrubs). Our key findings reveal that microbial communities in S. oritrepha soils were primarily C- and P-limited, with coexisting plants identity driving divergent limitation patterns: relative to pure stands, conifer coexistence (P. likiangensis) consistently alleviated C limitation across all soils and layers, whereas mixed shrub coexistence (S. angustata) intensified C limitation (except for an alleviating effect in the mineral-layer rhizosphere). For P limitation, conifer coexistence strengthened limitation only in bulk soil, while mixed shrub coexistence primarily amplified P limitation in the rhizosphere. Soil moisture emerged as the dominant driver: it correlated positively with C limitation and negatively with P limitation. These results demonstrate that coexisting plants determines the direction and compartment-specificity of microbial resource limitation under shrub expansion—providing a functional framework to predict soil biogeochemical responses to alpine vegetation shifts, with critical implications for ecosystem management under global change.

青藏高原高山灌丛扩张重构了植物群落和土壤生物地球化学，但不同的植物共存模式（灌木针叶树与混合灌木）如何影响根际土壤和土层的微生物代谢限制仍未解决-限制生态系统对植被变化的响应预测。本研究通过胞外酶化学计量学和媒介物特性，定量分析了三种不同共存模式下生长的大柳（Salix oritrepha）灌木块根际土壤（有机/矿物层）微生物代谢限制。三种共存模式分别是：纯林分、与林分混交林、与林分混交林混交林。研究结果表明，古石斛土壤的微生物群落主要是限制C和p，共存的植物身份驱动不同的限制模式：相对于纯林分，针叶林共存（P. likiangensis）在所有土壤和层中都持续缓解了C限制，而混合灌木共存（S. angustata）则加剧了C限制（除了在矿物层根际有缓解作用）。对于磷的限制，针叶林的共存只增强了块状土壤的限制，而混合灌木的共存主要增强了根际磷的限制。土壤湿度是主要驱动因素，与碳限制呈正相关，与磷限制负相关。这些结果表明，共存植物决定了灌木扩张下微生物资源限制的方向和区间特异性，为预测高寒植被变化下土壤生物地球化学响应提供了一个功能框架，对全球变化下的生态系统管理具有重要意义。

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引用次数: 0

Season-specific cutting strategies enable efficient clonal propagation of Berberis amurensis Rupr 特定季节的采伐策略使小檗无性系繁殖高效

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2026-01-13 DOI: 10.1016/j.rhisph.2026.101268

Gun Mo Kim , Suejin Park , Seung Youn Lee

Berberis amurensis Rupr. is a pollinator-supporting shrub valued for its medicinal and ornamental traits; however, practical propagation is limited due to slow seed germination and a lack of vegetative propagation methods. This study investigated the impact of cutting season, auxin concentration, and cutting type on the adventitious rooting of stem cuttings. Hardwood cutting rooted in May showed higher survival, rooting percentage, and root system development than those rooted in August, and potassium indole-3-butyric acid at 500–1000 mg L⁻¹ increased root biomass and root number in May. In August, greenwood cuttings rooted and survived better than hardwood cuttings, even without auxin treatment. Anatomical observations confirmed that the adventitious root originated from the cambial zone and reconnected with the stem vascular tissues. These findings establish simple and season-specific protocols for the clonal propagation of B. amurensis for ex situ conservation and horticultural use.

小檗。是一种具有药用和观赏价值的传粉灌木；然而，由于种子发芽缓慢和缺乏无性繁殖方法，实际繁殖受到限制。研究了扦插季节、生长素浓度和扦插方式对茎条扦插不定根的影响。5月生根的硬木扦插成活率、生根率和根系发育均高于8月，500 ~ 1000 mg L−1的吲哚-3-丁酸钾增加了5月的根系生物量和根数。8月，即使没有生长素处理，绿木扦插生根和成活率也比硬木扦插好。解剖观察证实不定根起源于形成层区，并与茎维管组织重新连接。这些发现建立了简单和季节特异性的克隆繁殖方案，用于移地保护和园艺利用。

引用次数: 0

A new strain of Trichoderma shows improved biocontrol of Fusarium root rot in ginseng via functional metabolites, defense induction and rhizosphere regulation 一株新菌株木霉通过代谢物、防御诱导和根际调控对人参镰刀菌根腐病有较好的防制效果

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2026-01-13 DOI: 10.1016/j.rhisph.2025.101237

Simeng Zhao , Liwei Wang , Chao Li , Zhangxin Hou , Xinping Yang , Hongyan Yang

Fusarium oxysporum-induced Panax ginseng root rot is a devastating disease. The development of effective biocontrol agents is therefore crucial. This study isolated Trichoderma velutinum YW411 from ginseng rhizosphere soil and evaluated its biocontrol efficacy alone and in combination with Penicillium citrinum YW322. Pot experiments demonstrated that single inoculation with T. velutinum significantly inhibited root rot and promoted plant growth. The strain produced siderophores, indole-3-acetic acid, protease, and cellulase, but lacked 1-aminocyclopropane-1-carboxylate deaminase and phosphate-solubilizing capacity. Soil pH, total potassium and the activity of the defense-related enzyme lipoxygenase were significantly higher in the T. velutinum-only treatment compared to the co-inoculation treatment. Co-inoculation with P. citrinum altered microbial communities but diminished disease resistance. In vitro assays revealed that T. velutinum growth was inhibited by lipopeptides and other compounds secreted by P. citrinum, thereby reducing the ability of the ginseng rhizosphere biocontrol system with T. velutinum to suppress F. oxysporum spore germination and biomass. These results indicate that T. velutinum YW411 is a promising biocontrol and growth-promoting agent via direct pathogen inhibition, host resistance induction, and rhizosphere environment regulation, but it is incompatible with P. citrinum YW322.

尖孢镰刀菌引起的人参根腐病是一种具有毁灭性的病害。因此，开发有效的生物防治剂至关重要。本研究从人参根际土壤中分离得到绒毛木霉YW411，并对其单独及与柑橘青霉YW322联合抑菌效果进行了评价。盆栽试验表明，单次接种绒霉能显著抑制根腐病，促进植株生长。菌株产生铁载体、吲哚-3-乙酸、蛋白酶和纤维素酶，但缺乏1-氨基环丙烷-1-羧酸脱氨酶和磷酸溶解能力。土壤pH、全钾和防御相关酶脂氧合酶活性均显著高于共接种处理。柠檬酸卟啉菌共接种改变了微生物群落，但降低了抗病性。体外实验结果表明，黄皮霉分泌的脂肽等化合物可抑制黄皮霉的生长，从而降低了黄皮霉在人参根际防制体系中抑制尖孢霉孢子萌发和生物量的能力。这些结果表明，T. velutinum YW411通过直接抑制病原菌、诱导寄主抗性和调节根际环境，是一种有前景的生物防治和促生长剂，但与P. citriinum YW322不相容。

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引用次数: 0

The key predictive factors of arbuscular mycorrhizal fungi community spatial distribution in saline-alkaline meadow soils: Abiotic variables or hosts? 盐碱草甸土壤丛枝菌根真菌群落空间分布的关键预测因子：非生物变量还是寄主？

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2026-01-10 DOI: 10.1016/j.rhisph.2026.101266

Lin Zhang , Yuqiang Wen , Mengmeng Zhang , Kun Li , Ruotong Wu , Ning Sun , Fuqiang Song , Tianle Xu

Arbuscular mycorrhizal (AM) fungi are vital plant symbionts in terrestrial ecosystems, yet their community distribution patterns and key drivers in extreme environments remain elusive. Focusing on the unique soda saline-alkaline meadows in the cold region of Northeast China, we established six sampling sites along a 500-km climatic gradient. By integrating traditional microscopic techniques with high-throughput sequencing, we systematically characterized the spatial distribution and drivers of soil AM fungal communities under severe saline-alkaline stress. Results indicated that under saline-alkaline stress, abiotic variables overrode host preferences to dominate community structuring. Specifically, high pH significantly suppressed root colonization and extraradical mycelial density, and significantly reduced soil AM fungal Shannon diversity. Nitrate nitrogen (NO₃⁻-N) acted as a critical "diversity filter," showing a significant negative correlation with species richness and phylogenetic diversity. Conversely, soil organic carbon (SOC), total nitrogen (TN), and regional climate synergistically drove community composition divergence. Crucially, the lack of significant correlation between soil AM fungal and plant communities confirmed the primacy of abiotic filtering in these extreme habitats. Further analysis revealed divergent ecological strategies: Regional climate potentially regulated the abundance of the dominant genera Glomus and Scutellospora, whereas Rhizophagus was driven by local nutrient-rich micro-niches (SOC and ammonium nitrogen) and closely associated with plant productivity. This study identifies severe soil abiotic stress as the core driver shaping AM fungal spatial patterns in cold saline-alkaline meadows. These findings deepen our understanding of microbial distribution in stressed habitats and offer critical scientific insights for the restoration of fragile saline-alkaline ecosystems.

丛枝菌根真菌（AM）是陆地生态系统中重要的植物共生体，但其群落分布模式和极端环境下的关键驱动因素尚不清楚。以东北寒区独特的碱盐碱草甸为研究对象，沿500 km的气候梯度建立了6个采样点。通过将传统显微技术与高通量测序技术相结合，系统表征了严重盐碱胁迫下土壤AM真菌群落的空间分布特征及其驱动因素。结果表明，在盐碱胁迫下，非生物变量凌驾于寄主偏好之上，主导群落结构。高pH显著抑制根定植和根外菌丝密度，显著降低土壤AM真菌Shannon多样性。硝态氮（NO3−-N）是重要的“多样性过滤器”，与物种丰富度和系统发育多样性呈显著负相关。相反，土壤有机碳（SOC）、全氮（TN）和区域气候协同驱动群落组成差异。至关重要的是，土壤AM真菌与植物群落之间缺乏显著相关性，证实了在这些极端栖息地中非生物过滤的首要地位。区域气候对优势属Glomus和Scutellospora的丰度有潜在的调控作用，而Rhizophagus则受当地富营养微生态位（SOC和铵态氮）的驱动，并与植物生产力密切相关。本研究认为，严重的土壤非生物胁迫是影响冷盐碱草甸AM真菌空间格局的核心驱动因素。这些发现加深了我们对逆境生境中微生物分布的理解，并为脆弱的盐碱生态系统的恢复提供了重要的科学见解。

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