Rhizosphere最新文献

{"title":"Physicochemical properties and fungal communities of forest soil combine to influence ginseng rusty root grade","authors":"Siwei Qiao ,&nbsp;Hang Xu ,&nbsp;Zheng Li ,&nbsp;Xiaolin Chen ,&nbsp;Shuxin Li ,&nbsp;Shiquan Xu ,&nbsp;Hao Zhang","doi":"10.1016/j.rhisph.2025.101050","DOIUrl":"10.1016/j.rhisph.2025.101050","url":null,"abstract":"<div><div>Ginseng rusty root (GRR) disease is a common ginseng disease that affects the quality and value of ginseng. Although some studies have focused on the mechanism of GRR incidence in farmland, little is known in forest conditions. The objective of this study was to clarify how GRR grades correlate with the physicochemical properties and fungi in forest soils. After examination of 38 sites in Changbai Mountain Range, 8 sites were selected for sampling, guided by their GRR gradings. The soil properties of forest soil samples were analyzed, and ITS-1 sequencing of the rhizosphere fungi was performed. The sequencing results were subjected to multivariate statistical analysis to explore GRR indicators. The results showed that soil pH, total nitrogen, total phosphorus, soil organic carbon, and moisture content varied significantly among 8 sampling sites. Total phosphorus and soil organic carbon were positively correlated with GRR grade. High GRR grade soils showed extremely high moisture content and low pH. At the phylum level, the abundances of Mortierellomycota, Rozellomycota, and Ascomycota were higher in high GRR grade soils. At the species level, <em>Roesleria subterranea</em> and <em>Ilyonectria mors-panacis</em> were abundant in high GRR grade soils. Overall, the grade of GRR is influenced by a combination of soil properties and the fungal community present in the soil. This study suggests that soil physicochemical properties and certain <em>Ilyonectria</em> spp. can be used as indicators for GRR grade. These scientific information will aid in selecting forest fields for ginseng planting.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101050"},"PeriodicalIF":3.4,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of arbuscular mycorrhizal inoculation and MoS2 nanoparticles amendment on coriander growth and cadmium uptake in Cd-contaminated soil","authors":"Xu Chen ,&nbsp;Juhong Yang ,&nbsp;Zixin Zhou ,&nbsp;Jiahong Zuo ,&nbsp;Xiaoyu Zheng ,&nbsp;Jingping Gai","doi":"10.1016/j.rhisph.2025.101037","DOIUrl":"10.1016/j.rhisph.2025.101037","url":null,"abstract":"<div><div>The presence of heavy metals in soil, particularly cadmium (Cd), poses a significant risk to soil health, crop growth, and human welfare due to its persistence and bioaccumulation in the food chain. To effectively address this issue through remediation techniques, further research is necessary on integrating microorganisms with advanced materials such as nanoparticles. A pot experiment was conducted to evaluate the impact of molybdenum disulfide (MoS₂) nanoparticles (NPs) application, arbuscular mycorrhizal fungi (AMF) inoculation, and their combinations on coriander (<em>Coriandrum sativum</em>) growth and Cd uptake. The results demonstrated that both MoS₂ NPs application and AMF inoculation significantly reduced Cd accumulation in plant tissues while increasing plant biomass. The primary effect observed from 50 mg kg⁻¹ MoS₂ NPs is an increase in shoot Mo and P content and antioxidant enzyme activity. Notably, compared to MoS₂ NPs, AMF alone exhibits superior alleviation of Cd stress through increased Peroxidase (POD) activity, enhanced P uptake, and improved chlorophyll content. Under low Cd conditions, the combined application of AMF and MoS₂ NPs demonstrated a synergistic effect in reducing malondialdehyde (MDA) content. However, consistent synergistic effects on plant growth and Cd uptake were not observed from this combination possibly due to interactions between the two treatments. This study highlights the potential integration of MoS₂ NPs with AMF inoculants in soil remediation strategies, while emphasizing the need for further research to identify suitable host plants and optimize their interactions for overall effectiveness.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101037"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of antifungal and nitrogen-fixing activities of Niallia circulans YRNF1 using response surface methodology to control Fusarium root rot of pepper and promote the plant growth","authors":"Mohamed Hafez ,&nbsp;Younes M. Rashad ,&nbsp;Omar M. Ibrahim ,&nbsp;Hany H.A. El-Sharkawy ,&nbsp;Ahmed M. Abd-Elgawad ,&nbsp;Mohamed Bourouah ,&nbsp;Sara A. Abdalla ,&nbsp;Mohamed M. Sleem ,&nbsp;Adel K. Madbouly","doi":"10.1016/j.rhisph.2025.101049","DOIUrl":"10.1016/j.rhisph.2025.101049","url":null,"abstract":"<div><div>Sweet pepper crop is exposed to Fusarium root rot, caused by <em>Fusarium solani</em> (Mart.) Sacc., resulting in great economic losses. In this study, culturing conditions for nitrogen-fixing and biocontrol activities of <em>Niallia circulans</em> YRNF1 were optimized in vitro using the response surface methodology. Results indicated that the optimum culturing conditions for both activities were initial pH = 6, glucose concentration of 27.5 mgL⁻¹, temperature of 13.5 °C, and incubation time of 12.3 days. In the greenhouse, inoculating the infected pepper plants with <em>N. circulans</em> YRNF1, grown under optimized conditions, reduced the disease severity to 24.8%, compared to 37.3% in case of the un-optimized <em>N. circulans</em> YRNF1. In addition, three defense-related genes (<em>CHI II</em>, <em>C4H</em>, and <em>H3F</em>) were highly overexpressed more than in case of the un-optimized bioagent. Treating the infected plants with <em>N. circulans</em> YRNF1, grown under the optimized conditions, triggered the plant defense responses through inducing the phenolic compounds accumulation and activities of two antioxidant enzymes; POD and PPO. Inoculating the infected plants with the optimized <em>N. circulans</em> YRNF1 promoted their shoot height and root length (22.00 and 9.00 cm, respectively) and the flowers number, compared to the untreated infected plants (17.33 and 4.00 cm, respectively). Moreover, application of the optimized <em>N. circulans</em> YRNF1 on the pepper plants led to a significant increment in the total nitrogen content in pepper roots (206.97%), compared to those inoculated with the un-optimized bioagent (181.39%). Optimization of the culturing conditions of <em>N</em>. <em>circulans</em> YRNF1 represents a promising and eco-friendly approach for supporting the organic and sustainable agriculture.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101049"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil acidification reduces flavonoids and key metabolites in sugarcane roots and rhizosphere leading to yield decline","authors":"Ziqin Pang ,&nbsp;Lifang Mo ,&nbsp;Qiang Liu ,&nbsp;Qianying Huang ,&nbsp;Yijie Xiao ,&nbsp;Zhaonian Yuan","doi":"10.1016/j.rhisph.2025.101028","DOIUrl":"10.1016/j.rhisph.2025.101028","url":null,"abstract":"<div><div>Soil acidification has negative effects on sugarcane growth, leading to yield reduction. However, little is known about its effects on metabolite composition and functional pathways in sugarcane roots and soil. This study used untargeted metabolomics techniques to analyze metabolite changes in sugarcane roots, rhizosphere soil and bulk soil from highly acidified sugarcane fields in Laibin, Guangxi. Our results showed that the progression of soil acidification not only intensifies soil compaction, resulting in elevated soil bulk density and decreased porosity, but also significantly impacts soil nutrient content adversely. Specifically, soil acidification leads to a decrease in available potassium content by 144% and 123%, respectively, while the concentrations of organic matter and available nitrogen in the rhizosphere soil decrease by 33.09% and 22.45%, respectively. At the metabolic level, we observed a significant decrease in the overall classification of flavonoids in sugarcane roots and rhizosphere soil in acidified fields. Specifically, metabolites such as cellotetraose and levan decreased in sugarcane roots, rhizosphere and bulk soil, while gluconic acid and S-lactoylglutathione were reduced in both sugarcane roots and rhizosphere soil. In addition, catechin and chrysin were significantly reduced in sugarcane roots from acidified fields. Preliminary correlation analysis indicated a significant positive correlation between substances such as S-lactoylglutathione and levan and agronomic traits including sugarcane yield. This study provides a theoretical basis for understanding the mechanisms by which soil acidification affects sugarcane and provides guidance for sustainable sugarcane production.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101028"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen management in rice under crop rotation and nitrogen level adjustment: Comprehensive responses of soil, roots, and plant growth","authors":"Yunsheng Song ,&nbsp;Minghui Dong ,&nbsp;Meijuan Jin ,&nbsp;Junrong Gu ,&nbsp;Fei Chen ,&nbsp;Peifeng Chen ,&nbsp;Xiuliang Jin ,&nbsp;Yajie Hu ,&nbsp;Yuxuan Wang","doi":"10.1016/j.rhisph.2025.101038","DOIUrl":"10.1016/j.rhisph.2025.101038","url":null,"abstract":"<div><div>High-quality japonica rice is widely valued in Asia, particularly in China's Taihu Lake region, for its unique taste and nutritional qualities. However, the excessive application of nitrogen (N) fertilizers threatens both rice quality and environmental sustainability, demanding a reconsideration of traditional agricultural practices. This study investigates the combined effects of different crop rotation systems and N fertilizer rates on N management in rice cultivation. It focuses on soil N dynamics, as well as the structural and physiological responses of rice root systems and above-ground growth. A field experiment was conducted in the Taihu Lake region for two years, following a split-plot design that included two rotation patterns—rice-wheat and rice-morel—and two levels of N application: the conventional rate and a 10% reduction. The cultivar ‘Suxiangjing 100’ was used. Variables such as soil inorganic N content, root morphology, root H⁺ and NO₃^– uptake, N-metabolizing enzyme activities, and plant biomass and N accumulation were systematically analyzed. The rice-morel rotation combined with a 10% reduction in N markedly increased both nitrate and ammonium N contents in the soil, particularly in the 0–20 cm and 20–40 cm layers. This treatment also significantly enhanced root length, surface area, and volume, especially in the topsoil layer. Root tips exhibited a greater capacity for H⁺ and NO₃^– uptake, especially in shallow soil. Furthermore, this treatment substantially boosted nitrate reductase (NR) and glutamine synthetase (GS), especially during the heading and maturation stages, with enzyme activities significantly higher (<em>p</em> &lt; 0.05). Both plant biomass and N accumulation were markedly higher, demonstrating improved growth and nitrogen use efficiency (NUE). Correlation analyses indicated a significant positive relationship between root morphology and N absorption capacity in the top 10 cm of soil (<em>p</em> &lt; 0.01). Adjusting crop rotation patterns and N application rates can significantly enhance soil N status, optimize root system morphology, and improve plant growth and N accumulation, thereby enhancing N management in rice. Overall, the rice-morel rotation with a 10% N reduction demonstrated strong potential for optimizing rice's overall N utilization and growth performance.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101038"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil nutrients, rhizosphere bacteria, and endophytes interact to influence the accumulation of amino acid-short peptide components in Angelica sinensis roots, each playing distinct roles","authors":"Xiaopeng Guo ,&nbsp;Shengli Zhang ,&nbsp;Runsheng Yin ,&nbsp;Shuhua Zhu ,&nbsp;Bin Ji ,&nbsp;Yinan Peng ,&nbsp;Shuhan Zhang ,&nbsp;Xiuyue Xiao ,&nbsp;Cheng Peng ,&nbsp;Yonggang Wang","doi":"10.1016/j.rhisph.2025.101027","DOIUrl":"10.1016/j.rhisph.2025.101027","url":null,"abstract":"<div><div>Improving the medicinal quality of <em>Angelica sinensis</em> root (Ang) through microecological regulation is crucial; however, the factors shaping the active ingredients require further exploration. This study simultaneously examined the soil physicochemical properties, the rhizosphere-endophytic bacterial communities, and the metabolic components of Ang from various authentic planting sites, followed by an association analysis. Three representative sites were highlighted: Site #1 had the lowest soil nitrogen-phosphorus-potassium contents, while Site #2 exhibited the highest levels; Site #3 was moderate but demonstrated dominant organic carbon due to high soil sucrase activity. Corresponding to the soil conditions, the abundance of rhizosphere-endophytic beneficial bacteria at Site #1 was the lowest. In contrast, Sites #2 and #3 showed dominance in rhizosphere bacteria and endophytes, respectively. Regarding active ingredients, Ang from Site #1 primarily accumulated host defense-related amino acids and derivatives. The abundance of short peptides was highest in Ang from Site #2, whereas Site #3 had the highest relative content of total amino acids, short peptides, and derivatives (AASPD). Mathematical fitting indicated that under the synergism of soil conditions, rhizosphere bacteria (such as <em>Flavobacterium</em>, <em>Stenotrophomonas</em>, and <em>Paracoccus</em>) had the most significant effect on overall AASPD (path coefficient = 0.806, <em>p</em> = 0.0006). In contrast, endophytic bacteria (such as <em>Parasutterella</em>, <em>Parabacteroides</em>, and <em>Bacteroides</em>) specifically influenced short peptides, with path coefficients and <em>p</em>-value of 1.035 and 0.0006, respectively. Overall, key microecological factors interact with one another, shaping the AASPD components in Ang with varying effects. These findings can provide references for enhancing the quality of Ang through microecological regulation.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101027"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the microbial mysteries of mulberry rhizosphere in saline-alkaline soils","authors":"Na Li ,&nbsp;Jisheng Li ,&nbsp;Shengjie Zhang ,&nbsp;Xiaojun Lan ,&nbsp;Heng Zhou","doi":"10.1016/j.rhisph.2025.101040","DOIUrl":"10.1016/j.rhisph.2025.101040","url":null,"abstract":"<div><div>Mulberry is one of the top five global plants and is essential for managing saline-alkali soils and offering superior nutritional value for livestock. However, research on the archaeal, bacterial, and fungal communities in the rhizosphere soil of mulberry under saline-alkali conditions is limited. This study aims to elucidate the distribution characteristics of microbial communities in the mulberry rhizosphere across different soil layers using non-cultivation methods. The microbial community composition was analyzed through 16S rRNA and ITS gene sequencing from rhizosphere soil samples collected at layers ranging from 0 to 100 cm. Diversity analysis revealed significant differences in the diversity of archaea and bacteria across different soil layers, while fungal diversity remained consistent. Taxonomic analysis indicated that the dominant archaeal phyla were <em>Crenarchaeota</em> and <em>Thermoplasmatota</em>, while the main bacterial phyla were <em>Proteobacteria</em>, <em>Actinobacteriota</em>, <em>Acidobacteriota</em>, <em>Chloroflexi</em>, and <em>Bacteroidetes</em>. Fungi, the primary phyla identified were <em>Ascomycota</em> and <em>Mortierellomycota</em>. Notably, many of these taxa are considered beneficial for growth promotion and biological control. Linear Discriminate Analysis (LDA) and Linear Discriminant Analysis Effect Size (LEfSe) revealed 15, 29, and 7 biomarker microbes in the five soil layers, respectively. The biomarkers in different soil layers were mainly concentrated in single phyla or classes, suggesting that microbes with similar biological functions may survive across varying soil layers. The networks were dominated by positive correlations (98.45% of total edges), with strong connections between the upper soil layers. Archaeal diversity was significantly correlated with total carbon, nitrogen, and protein content in the soil, while fungal diversity was significantly correlated with available potassium content. No significant correlation was found between environmental factors and bacterial diversity. Redundancy analysis based on the top 20 genera and all ASV levels showed that different taxonomic levels were influenced by different environmental factors, with TK being the primary environmental factor affecting bacteria and EC influencing fungal communities. Additionally, the main functional profiles of archaea and bacteria were primarily nitrogen-related and carbon-related functional groups, while the most common functional groups for fungi were saprotrophs, plant pathogens, and wood saprotrophs. These findings suggest that the rhizosphere microbes associated with mulberry have significant potential for exploring microbial dark matter and resources, particularly in the remediation of saline-alkaline soils.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101040"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The addition of rhizosphere soil can affect the plant-soil-microbial nutrients of Bothriochloa ischaemum","authors":"Yong Cao ,&nbsp;Zemin Ai ,&nbsp;Xiaohu Dang ,&nbsp;Qiuji Chen ,&nbsp;Tao Wang ,&nbsp;Jiayi Li ,&nbsp;Yi Deng ,&nbsp;Huan Liu ,&nbsp;Qingqing Li ,&nbsp;Mengjie Zhao","doi":"10.1016/j.rhisph.2025.101042","DOIUrl":"10.1016/j.rhisph.2025.101042","url":null,"abstract":"<div><div>Secondary succession is an crucial way of vegetation restoration in grasslands, but it requires a slow process. Accelerating the process is receiving increased research attention. The rhizosphere soil addition (RSA) of plants growing in the late secondary succession may become an crucial tool for the ecological restoration of grassland. However, the response of plant–soil–microbe characteristics in degraded grasslands to RSA requires further investigation. Herein, the herb <em>Bothriochloa ischaemum</em> typical of the late secondary succession in the loess area and the soil to be reclaimed in the mining area were used to test the effects of adding low (RSA1), medium (RSA2), high (RSA3) concentrations of rhizosphere soil on plant–soil–microbial properties through potting experiments. Results showed that RSA1, RSA2, and RSA3 significantly increased the biomass of <em>Bothriochloa ischaemum</em> leaves (74%, 117%, and 245%), stems (99%, 108%, and 394%), roots (58%, 133%, and 223%), and total biomass (77%, 119%, and 293%). However, it had no effect on plant or soil carbon content. RSA significantly increased only soil total phosphorus, soil available nitrogen, and water-soluble phosphorus. RSA3 significantly increased plant carbon content, whereas RSA1 and RSA2 decreased plant carbon content. RSA reduced the intensity of plant nitrogen limitation and increased the intensity of phosphorus limitation. RSA1 and RSA2 can also significantly increase the microbial nitrogen–phosphorus ratio; different intensities of RSA cannot significantly affect the microbial carbon–phosphorus ratio but can reduce the soil enzyme stoichiometry. RSA can mediate plant–soil–microbial nutrients, thereby promoting the recovery of grassland ecosystems.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101042"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inoculation of soybean rhizosphere microorganisms could promote corn growth","authors":"Siqi Yu,&nbsp;Yi Shen,&nbsp;Yaohui Yang,&nbsp;Zhenyan Zhang,&nbsp;Jichao Zhu,&nbsp;Qingshan Xia,&nbsp;Minglong Song,&nbsp;Binghai Lv,&nbsp;Liwei Sun,&nbsp;Haifeng Qian,&nbsp;Tao Lu","doi":"10.1016/j.rhisph.2025.101047","DOIUrl":"10.1016/j.rhisph.2025.101047","url":null,"abstract":"<div><div>Rhizosphere microbes are essential for crop growth and development. Soybean rhizosphere microbes are believed to have nitrogen-fixing functions that promote plant growth. However, it is not known whether soybean rhizosphere microbes promote the growth of other crops via transplantation. In this study, we investigated the potential of soybean-derived rhizosphere microbes to promote corn growth, included a significant increase (5.3%) in corn plant height. The inoculation of soybean rhizosphere microbes did not significantly affect microbial diversity in the corn rhizosphere, but the relative abundance of plant-beneficial bacteria increased significantly by 23.12%, particularly the genera <em>Nocardioides</em>, <em>Variovorax</em>, <em>Pseudoalteromonas</em>, <em>Bosea</em> and <em>Adhaeribacter</em>. Furthermore, inoculation with soybean rhizosphere microbes promoted the expression of metabolic genes involved in carbohydrate metabolism, amino acid metabolism, and membrane transport, which in turn accelerated nutrient cycling in the soil and promoted plant growth. Our study shows that soybean can enrich many beneficial plant bacteria in rhizosphere whose function is beyond nitrogen fixation, and these enriched beneficial bacteria can be applied to other crops, such as corn, by transferring rhizosphere soil, providing new insights into rhizosphere microbial inoculation techniques and contribute to our understanding of the ecological functions associated with rhizosphere microbes.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101047"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of integrated nutrient management on soil microbiome diversity and health in rice based cropping system: Insights from long-term agricultural practices","authors":"Subhojit Datta ,&nbsp;Sonali Paul Mazumdar ,&nbsp;Bijan Majumdar ,&nbsp;N.M. Alam ,&nbsp;Lipi Chattopadhyay ,&nbsp;Sourav Ghosh ,&nbsp;Dipnarayan Saha ,&nbsp;Amit Ranjan Saha ,&nbsp;Gouranga Kar","doi":"10.1016/j.rhisph.2025.101048","DOIUrl":"10.1016/j.rhisph.2025.101048","url":null,"abstract":"<div><div>The rhizosphere soil microbiomes, which are essential for plant development, stress adaptability, and general soil health, are greatly impacted by agricultural management practices, particularly those involving nutrient applications. This study evaluated the long-term effects of nutrient management practices on soil physicochemical properties, microbial communities, enzyme activities, and biological soil health in a rice-lentil-jute cropping system. The treatments included a control, inorganic fertilizers (recommended dose of fertilizers), and Soil Test Crop Response based integrated nutrient management (combination of inorganic fertilizer based on soil test-based fertilizer prescription equations, farmyard manure (FYM), and bioinoculants). When compared to both control and inorganic treatments, integrated nutrient management (INM) enhanced soil organic carbon, available nitrogen, phosphorus, and potassium. Microbial populations, comprising of bacteria, actinomycetes, fungi, <em>Azotobacter</em>, and phosphate-solubilizing microorganisms, along with soil enzymatic activities, showed marked increases under INM. Metagenomic analysis of the hypervariable V3-V4 region of 16S rRNA indicated that the bacterial community in the rice-lentil-jute cropping sequence was dominated by <em>Proteobacteria</em>, with 58 phyla having over 1% abundance. The INM treatment increased the Shannon diversity index by 12.6% compared to the control, reflecting improved microbial diversity, richness, and resilience, which are critical for enhancing crop productivity and stress tolerance. The Biological Soil Health Index (BSHI) was highest in the INM treatment, with average contributions from <em>Azotobacter</em> (22.7%), <em>Bacteroidota</em> (12.1%), <em>Actinobacteriota</em> (21.9%)<em>,</em> very labile organic carbon (23.1%)<em>,</em> and labile organic carbon (20.2%) to BSHI. In summary, ten years of INM enhanced soil health and bacterial community structure and composition, leading to sustainable crop yields in rice-based cropping system. These findings highlight the necessity of integrating balanced nutrient management in long-term agricultural practices.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101048"},"PeriodicalIF":3.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}