Rhizosphere最新文献

{"title":"Protected cultivation of the stock plant enhances rooting of wounded cuttings of Caryocar brasiliense (Caryocaraceae)","authors":"Adriene Matos dos Santos ,&nbsp;Hellen Cássia Mazzottini-dos-Santos ,&nbsp;Leonardo Monteiro Ribeiro ,&nbsp;Renan Ribeiro Silva ,&nbsp;Nermy Ribeiro Valadares ,&nbsp;Paulo Sérgio Nascimento Lopes","doi":"10.1016/j.rhisph.2025.101254","DOIUrl":"10.1016/j.rhisph.2025.101254","url":null,"abstract":"<div><div>Little is known about the factors controlling the efficiency of vegetative propagation by cuttings in tropical woody species. <em>Caryocar brasiliense</em>, an endemic fruit tree of the Cerrado biome, shows great potential for domestication through this technique. This study examined anatomical and physiological aspects related to the effects of the cultivation environment of the stock plants and the timing of wounding at the base of cuttings on the success of propagation. Stock plants were cultivated under full sunlight, shade netting, and greenhouse conditions, while cuttings were wounded at their bases at 0, 7, and 14 days after cutting. Rooting, morphoanatomy, and physiology of both stock plants and cuttings were evaluated, together with the ontogeny of adventitious roots. Greenhouse cultivation promoted greater growth, higher photosynthetic efficiency, and increased carbohydrate concentrations, as well as reduced lignification and phenolic compound accumulation in the cortical region. The phytohormones indole-3-acetic acid and jasmonic acid, together with peroxidase enzyme activity and the ratios of indole-3-acetic acid to 1-aminocyclopropane-1-carboxylic acid, peroxidase, and abscisic acid, showed strong positive correlations with rooting, whereas abscisic acid and zeatin showed negative correlations. Cultivation under 70 % shading and wounding applied seven days after cutting favored adventitious root formation. Adventitious root ontogeny in <em>C. brasiliense</em> is multisite, originating from the vascular cambium, phloem, cortex, and callus, depending on the cutting's origin in relation to the stock plant's cultivation environment. These findings provide insights into optimizing the propagation of <em>C. brasiliense</em> for domestication and conservation purposes.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101254"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root exudate-microbiota interaction: Novel strategies for sustainable crop disease control","authors":"Xiuyun Zhao,&nbsp;Chenyang Du,&nbsp;Qiang Zeng,&nbsp;Yue Kang,&nbsp;Gaofu Qi","doi":"10.1016/j.rhisph.2026.101262","DOIUrl":"10.1016/j.rhisph.2026.101262","url":null,"abstract":"<div><div>Root exudates—up to one-fifth of plant-fixed carbon—function as the rhizosphere's universal currency, simultaneously mobilising nutrients, signalling to microorganisms, and erecting chemical defense. Here, we synthesize recent studies to demonstrate how this metabolite cocktail (amino acids, organic acids, sugars, phenylpropanoids, terpenoids, alkaloids, and peptides) is dynamically reconfigured by genotype, developmental stage, soil type, nutrient status, drought, salinity, temperature, and pathogen attack. The plant-controlled shift in exudate composition feeds, chemoattracts or repels specific microbial taxa, creating a beneficial microbiome that solubilises minerals, fixes nitrogen, induces systemic resistance and outcompetes pathogens, thereby self-engineering a healthier, more resilient soil ecosystem. Conversely, pathogens exploit the same exudate gradients for chemotaxis and infection, forcing plants to mount a rapid, targeted secretion of antimicrobials and defence-associated compounds. We highlight critical gaps: (i) absence of field-realistic, microbe-sparing collection protocols; (ii) limited knowledge of biosynthetic and transport proteins dictating metabolite export; (iii) under-explored perception of exudates by fungi, viruses and nematodes. Bridging these gaps via portable sampling devices, multi-omics and genome editing will convert root exudates from descriptive metabolites into predictable, breedable traits, enabling low-input crops that engineer their own microbiome to enhance nutrient acquisition, stress tolerance and disease resistance.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101262"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Season-specific cutting strategies enable efficient clonal propagation of Berberis amurensis Rupr","authors":"Gun Mo Kim ,&nbsp;Suejin Park ,&nbsp;Seung Youn Lee","doi":"10.1016/j.rhisph.2026.101268","DOIUrl":"10.1016/j.rhisph.2026.101268","url":null,"abstract":"<div><div><em>Berberis amurensis</em> Rupr. is a pollinator-supporting shrub valued for its medicinal and ornamental traits; however, practical propagation is limited due to slow seed germination and a lack of vegetative propagation methods. This study investigated the impact of cutting season, auxin concentration, and cutting type on the adventitious rooting of stem cuttings. Hardwood cutting rooted in May showed higher survival, rooting percentage, and root system development than those rooted in August, and potassium indole-3-butyric acid at 500–1000 mg L⁻¹ increased root biomass and root number in May. In August, greenwood cuttings rooted and survived better than hardwood cuttings, even without auxin treatment. Anatomical observations confirmed that the adventitious root originated from the cambial zone and reconnected with the stem vascular tissues. These findings establish simple and season-specific protocols for the clonal propagation of <em>B. amurensis</em> for ex situ conservation and horticultural use.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101268"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benzoxazinoids and plant growth-promoting bacteria: A pathway to sustainable agriculture","authors":"Jeroen Baatsen,&nbsp;João L. Azevedo,&nbsp;Maria C. Quecine","doi":"10.1016/j.rhisph.2026.101264","DOIUrl":"10.1016/j.rhisph.2026.101264","url":null,"abstract":"<div><div>Grass plants influence the composition of their rhizobiome through the secretion of metabolites, such as benzoxazinoids (BXs), which shape microbial communities. Paramount to plant health, the root associated microbiome may confer plant growth-promoting effects and tolerance to pathogens and herbivorous insects. Specifically, the BX derivative 6-methoxy-2-benzoxazolinone (MBOA), exhibits prolonged effects on soil microbiota and plant defense mechanisms by sustained biosynthesis and its relatively stable molecular structure. Leveraging Plant Growth-Promoting Rhizobacteria (PGPR) offers a sustainable strategy to enhance soil fertility and crop yield while reducing reliance on chemical inputs. However, the efficacy of microbial inoculants is contingent upon various factors, including cultivar and environmental conditions, necessitating tailored approaches for successful implementation. The ecological impact BXs as plant signaling molecules can have on microbial ecology is demonstrated by experiments on <em>Fusarium</em> strains. Conditioning soil with MBOA may offer a promising strategy to enhance the efficacy of microbial inoculation, thus improving environmental conditions and crop cultivation outcomes. In this review, we discuss how BXs can be used as a tool in sustainable agricultural practices. Therefore, the biochemistry of BXs; the mechanisms of PGPR involved in root colonization; and plant-soil feedback are discussed, offering insights into optimizing crop management for enhanced sustainability, yield and pest tolerance.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101264"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arbuscular mycorrhizal symbiosis Drives rhizosphere-regulated drought tolerance in maize","authors":"Ghulam Murtaza ,&nbsp;Muhammad Usman ,&nbsp;Khairiah Mubarak Alwutayd ,&nbsp;Rashid Iqbal","doi":"10.1016/j.rhisph.2025.101250","DOIUrl":"10.1016/j.rhisph.2025.101250","url":null,"abstract":"<div><div>Drought-induced stress is a significant constraint for crop yields in semi-arid and arid areas.</div><div>Yield assessments under water stress indicate that mycorrhizae can alleviate the detrimental impacts of drought, placing them as sustainable options for agricultural practices in affected areas. Thus, we executed a two-year study to examine the effects of root colonization by two AMF species (<em>Diversispora epigaea</em> and <em>Diversispora versiformis</em>) under different drought stress conditions, assessing maize morpho-physiological and biochemical characteristics, nutrient absorption, yield components, oil percentage, and irrigation water efficiency. The research was conducted in a desolate region of Pakistan during the 2023 and 2024 growing seasons. Drought-induced stress was generated at two levels by irrigating after 80 % and 60 % water loss, categorized as severe and mild drought stress. Irrigation after a 40 % reduction in water was considered normal (without stress). The findings demonstrated that regardless of AMF species and level of drought stress, inoculated plants yielded heavier seeds, higher dry matter, chlorophyll (37 %) and carotenoids (41 %), phytohormone (27 %), enhanced oil yields (32 %) and seeds (24.2 %) compared to uninoculated plants. Notably, the maize seed yields of <em>Diversispora epigaea</em>-treated plants under every irrigation treatment surpassed those of <em>Diversispora versiformis</em> inoculated plants and uninoculated plants. Drought stress reduced nitrogen levels in seeds and leaves, whereas AMF enhanced nitrogen levels, particularly when crops were treated with <em>Diversispora epigaea</em>. Moreover, seed phosphorus percentages were not influenced by AMF in 2023. Conversely, the highest phosphorus percentages in seeds and leaves were recorded in crops inoculated with <em>Diversispora epigaea</em> in 2023. Our findings indicate that <em>Diversispora epigaea</em> exhibits greater efficiency under water stress and provides superior support to maize plants.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101250"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145841152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivar-dependent effects of arbuscular mycorrhizal (AM) fungal inoculation on fruit quality and native AM fungal community in navel orange","authors":"Xin-Ping Tan ,&nbsp;Cheng-Zhuo Li ,&nbsp;Ying-Ning Zou ,&nbsp;An-Qi Lei ,&nbsp;Mashael Daghash Alqahtani ,&nbsp;Qiang-Sheng Wu","doi":"10.1016/j.rhisph.2026.101269","DOIUrl":"10.1016/j.rhisph.2026.101269","url":null,"abstract":"<div><div>Given the importance of arbuscular mycorrhizal (AM) fungi in enhancing citrus resilience in low-fertility soils, understanding how AM fungal inoculation reshapes these symbiotic communities is critical for sustainable orchard management. This study investigated how targeted inoculation with <em>Diversispora spurca</em> and <em>D</em>. <em>versiformis</em> altered the native AM fungal populations in the roots and rhizospheres of ‘Lane Late’ and ‘Newhall’ navel orange trees, as well as the impact on fruit quality. Three years after inoculation, the effectiveness of the introduced AM fungi in boosting root AM colonization and improving fruit internal and external quality was cultivar-dependent, with <em>D</em>. <em>versiformis</em> favoring ‘Newhall’ and <em>D</em>. <em>spurca</em> excelling in ‘Lane Late’ through strain-specific modulation of sucrose metabolism pathways. Correlation study demonstrated that AM fungi enhanced fruit sugar metabolism and quality by up-regulating the expression of key sucrose-related genes, suggesting a link between the symbiotic colonization rate and improved fruit quality. A high-throughput sequencing investigation revealed that the ‘Newhall’ variety had more sequences and OTUs than the ‘Lane Late’ variety. Following AM fungal inoculation, the composition of the AM fungal community varied significantly between ‘Newhall’ and ‘Lane Late’, with cultivar-specific recruitment of dominant genera (<em>Paraglomus</em>, <em>Glomus</em>, and <em>Gigaspora</em> in ‘Newhall’ roots versus <em>Gigaspora</em> in ‘Lane Late’ soil) shaping distinct symbiotic profiles. AM fungal inoculation had contrasting effects on alpha diversity in roots and rhizosphere soil, with suppression in roots and enhancement in the soil, especially when <em>D</em>. <em>versiformis</em> was inoculated in ‘Newhall’. The AM fungal community composition was highly heterogenous. The differential associations of AM fungal genera with fruit quality—<em>Glomus</em> enhancing sugar levels while reducing acidity, versus <em>Paraglomus</em> and <em>Claroideoglomus</em> promoting acid retention—highlight host cultivar-specific trade-offs in fruit metabolism. These findings underscore the importance of AM fungal diversity as a driving factor in improving the quality of navel orange fruits.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101269"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoupled adaptation of plant microbiomes under global change: Partitioned responses and functional plasticity to warming and herbivory","authors":"Wenjing Chen ,&nbsp;Kelu Chen ,&nbsp;Huakun Zhou ,&nbsp;Li Ma","doi":"10.1016/j.rhisph.2025.101239","DOIUrl":"10.1016/j.rhisph.2025.101239","url":null,"abstract":"<div><h3>Background</h3><div>Multiple global change drivers frequently co-occur and interact, complicating predictions of ecosystem responses. However, an integrated understanding of how plant-associated microbiomes—spanning distinct host compartments and ecological niches—adjust to simultaneous climatic and biotic pressures remains limited.</div></div><div><h3>Results</h3><div>We conducted a randomized block split-plot field experiment in an alpine meadow, incorporating warming, simulated herbivory, and their combination, to examine bacterial and fungal communities associated with leaves and roots across both exogenous and endophytic fractions. High-throughput sequencing enabled a comprehensive evaluation of changes in community structure (diversity and composition), functional potential (predicted via PICRUSt2 and FUNGuild), and co-occurrence network characteristics. Three consistent patterns emerged: (i) marked spatially partitioned responses, wherein phyllosphere microbiomes—particularly exogenous assemblages—displayed high sensitivity to stressors (e.g., increased diversity, network simplification), whereas rhizosphere microbiomes exhibited greater resilience; (ii) community variation was driven predominantly by non-linear interactions between warming and herbivory rather than additive effects, with synergistic or antagonistic outcomes strongly dependent on the niche and ecological dimension; and (iii) a pervasive decoupling among community structure, functional potential, and network stability. For example, root endophytic networks were largely insensitive to combined stressors, yet predicted functional gene profiles changed markedly; conversely, leaf endophytic diversity remained stable even as functional potential was substantially reshaped.</div></div><div><h3>Conclusions</h3><div>These findings support an integrated “adaptive decoupling” framework, proposing that structural–functional decoupling serves as a central strategy enabling plant microbiomes to maintain resilience under multiple stressors. This mechanism allows communities to rapidly adjust functional capacities without altering overall structure, or to preserve core network stability during structural reorganization, thereby conferring substantial functional plasticity. The results challenge the conventional “structure determines function” paradigm and offer a more mechanistic basis for predicting ecosystem functional stability under accelerating global change.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101239"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enrichment and nitrogen preference of heterotrophic nitrifying bacteria in the maize rhizosphere","authors":"Mengqiu He ,&nbsp;Xiaoqian Dan ,&nbsp;Zichun Wang ,&nbsp;Zucong Cai ,&nbsp;Yi Zhang ,&nbsp;Jinbo Zhang ,&nbsp;Christoph Müller ,&nbsp;Yuanshuo Zhang ,&nbsp;Dejin Wang","doi":"10.1016/j.rhisph.2026.101257","DOIUrl":"10.1016/j.rhisph.2026.101257","url":null,"abstract":"<div><div>Heterotrophic nitrification (<em>O</em>_{<em>Nrec</em>}) is a crucial pathway of nitrate (NO₃⁻) production in acidic soils. While plants are known to stimulate <em>O</em>_{<em>Nrec</em>}, the specific microbial agents and their physiological traits remain largely unidentified. Building on our previous ¹⁵N tracing study, which quantified the stimulation of <em>O</em>_{<em>Nrec</em>} by maize, this research further identified the microbial drivers underpinning this phenomenon. Using plant removal and dark incubation treatments, we linked photosynthetic carbon flow to microbial dynamics. Most probable number (MPN) enumeration revealed significant enrichment of heterotrophic nitrifying bacteria and fungi in the rhizosphere, strongly correlated with soil dissolved organic carbon (DOC). We isolated multiple bacterial genera, including <em>Burkholderia</em>, <em>Stenotrophomonas</em>, <em>Bacillus</em>, <em>Sinomonas</em>, <em>Micromonospora</em>, and <em>Terrabacter</em>. These isolates exhibited remarkable metabolic versatility, oxidizing both organic (maize straw) and inorganic ammonium (NH₄⁺) to NO₃⁻. The findings demonstrate that maize plants selectively enrich heterotrophic nitrifiers via root exudates, thereby stimulating O_{<em>Nrec</em>} to enhance nitrogen availability.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101257"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aminochelates drive the reorganization of root-associated enzyme hotspots in the sunflower rhizosphere","authors":"Mina Alipourbabadi ,&nbsp;Mojtaba Norouzimasir ,&nbsp;Abdolamir Moezzi ,&nbsp;Afrasyab Rahnama ,&nbsp;Mehdi Taghavi ,&nbsp;Mehdi Rashtbari ,&nbsp;Bahar S. Razavi","doi":"10.1016/j.rhisph.2026.101299","DOIUrl":"10.1016/j.rhisph.2026.101299","url":null,"abstract":"<div><h3>Aims</h3><div>The mechanisms by which organic chelates influence rhizosphere enzyme dynamics and microbial function remain poorly understood, limiting our ability to predict their effects on nutrient cycling and root–microbe interactions. To address this gap, we evaluated the effects of iron (Fe) and zinc (Zn) aminochelates on the spatial distribution of β-glucosidase (βG) and leucine aminopeptidase (LAP) activities in the rhizosphere of sunflower (<em>Helianthus annuus</em> L.) using an integrated approach combining <em>in situ</em> zymography and biochemical assays.</div></div><div><h3>Methods</h3><div>Glycine- (Gly) and methionine-based (Met) Fe and Zn aminochelates were synthesized and applied in rhizobox experiments, with untreated soils serving as controls.</div></div><div><h3>Results</h3><div>[Fe (Gly)₂] and [Zn (Met)₂] significantly enhanced βG (7–21%) and LAP (72–120%) activities, while expanding enzymatic hotspot zones by 270–450% and 78–251%, respectively. Kinetic analyses showed that [Zn (Met)₂] achieved the highest catalytic efficiency (K_a) and maximum velocity (V_max, <em>p</em> &lt; 0.01), while also increasing basal respiration (+42.3%) and microbial biomass C (3-fold) relative to the control. Root length and surface area were strongly correlated with hotspot intensity (Pearson's <em>r</em> = 0.75–0.94), reflecting tight root–microbe feedbacks. Network analysis further revealed that [Fe (Met)₂] and [Zn (Met)₂] promoted the highest system-wide coordination, linking microbial enzyme activity with root architecture (Mantel's <em>r</em> = 0.56–0.68, <em>p</em> &lt; 0.05). By enhancing microbial activity, expanding biologically active zones, and improving root foraging traits, aminochelates appeared to influence both micronutrient availability and physiological responses in the rhizosphere, although direct measurements of metal speciation and plant uptake were not performed.</div></div><div><h3>Conclusions</h3><div>These results suggest that methionine-based aminochelates may influence soil fertility, nutrient cycling, and crop performance, although their efficacy relative to established approaches remains to be validated experimentally.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101299"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147395913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic plasticity of field pea (Lathyrus oleraceus L.) nodule morphology and cell-wall lignin in response to water and photothermal regimes","authors":"Michel Analia ,&nbsp;Adur Javier ,&nbsp;Caviglia Octavio Pedro ,&nbsp;Sadras Victor Oscar ,&nbsp;Ré Delfina Adela","doi":"10.1016/j.rhisph.2026.101273","DOIUrl":"10.1016/j.rhisph.2026.101273","url":null,"abstract":"<div><div>Legume plants associate symbiotically with <em>Rhizobium</em> and <em>Bradyirhizobium</em> bacteria that fix atmospheric nitrogen. Despite the well-documented effects of water regime on nodule establishment and functionality, little is known about its influence on nodule morphology after establishment. We grew <em>Lathyrus oleraceus</em> L. plants in a factorial experiment combining two growing conditions (winter and spring) and three levels of plant available water (PAW) in soil: 60 %, 85 %, and 100 %. Plant growth traits were measured. Nodules were analyzed with optical and confocal microscopy, using fluorescence lifetime imaging microscopy for lignin autofluorescence analysis. High water availability increased shoot biomass, as well as number and size of nodules compared to low water availability, with stronger effects in spring than in winter. The typical indeterminate nodules of field pea exhibited phenotypic plasticity whereby plants in dry soil had smaller and rounder nodules compared to plants grown at 100 % PAW. Lignin content of nodule cortex cells also varied with water and growing condition, being higher for 100 % PAW in winter and 60 % PAW in spring. Lignin content and morphological plasticity of field pea nodules may have implications for adaptation to water and photothermal variations.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"37 ","pages":"Article 101273"},"PeriodicalIF":3.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}