Rhizosphere最新文献_第6页

Changes in rhizospheric bacterial communities of the Andean shrub Fabiana densa in response to salinity 安第斯灌木Fabiana densa根际细菌群落对盐度的响应

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2025-12-03 DOI: 10.1016/j.rhisph.2025.101244

Cinthia Copeticona-Callejas, Isabel Morales-Belpaire

The expansion of quinoa cultivation areas in the Bolivian Altiplano has diminished the populations of native plants which play key ecological roles in the harsh environments of the high Andes. The shrub Fabiana densa not only thrives under extreme climatic conditions and nutrient-poor soils, but also shows tolerance to salinity. Among these species, the shrub Fabiana densa not only thrives under extreme climatic conditions and nutrient-poor soils, but it also shows tolerance to salinity. Understanding the adaptation mechanisms of F. densa to salinity could help revegetate salt-affected areas. Rhizobacteria, among other factors, may contribute to salinity tolerance of F. densa. Therefore, we aimed to assess how salinity affects the rhizospheric bacterial communities of F. densa, as a first step toward identifying bacterial families potentially involved in saline stress alleviation. We irrigated F. densa plants with 0, 15, 25, and 40 mM NaCl solutions under controlled conditions. After ten and twenty weeks of exposure to the salinity treatments, DNA was extracted from rhizospheric soil. The bacterial communities were analyzed by high-throughput sequencing of the V4 region of the 16S rRNA gene. Both salinity level and exposure time had a strong effect on the composition of the rhizospheric bacterial communities. After 10 weeks of exposure, differential abundances of Cyclobacteriaceae and Shewanellaceae were positively related to salinity. After 20 weeks of exposure, salinity caused a decrease in the differential abundances of Aeromonaceae and Rhodocyclaceae but an increase for Rhizobiaceae. The changes in community composition with time of sampling suggest that besides exposure to salinity, other environmental factors influenced bacterial communities and should be taken into account in further studies.

玻利维亚高原藜麦种植区的扩大减少了本土植物的数量，而这些植物在安第斯山脉的恶劣环境中起着关键的生态作用。灌木Fabiana densa不仅在极端的气候条件和贫瘠的土壤中茁壮成长，而且具有耐盐性。在这些物种中，灌木Fabiana densa不仅在极端气候条件和营养贫乏的土壤下茁壮成长，而且还表现出对盐度的耐受性。了解密螺旋藻对盐度的适应机制，有助于盐渍化地区的植被恢复。根细菌和其他因素可能有助于F. densa耐盐性。因此，我们旨在评估盐度如何影响F. densa根际细菌群落，作为鉴定可能参与盐胁迫缓解的细菌家族的第一步。在控制条件下，分别用0、15、25和40 mM NaCl溶液灌溉密莲植株。在盐处理10周和20周后，从根际土壤中提取DNA。采用16S rRNA基因V4区高通量测序分析细菌群落。盐度水平和暴露时间对根际细菌群落的组成有较强的影响。暴露10周后，环菌科和雪瓦菌科的差异丰度与盐度呈正相关。暴露20周后，盐度降低了气单菌科和红霉素科的差异丰度，但增加了根瘤菌科的差异丰度。群落组成随采样时间的变化表明，除了暴露于盐度外，其他环境因素也影响细菌群落，应在进一步的研究中加以考虑。

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引用次数: 0

Rhizosphere microbial recruitment and metabolite-mediated regulation drive contrasting salt tolerance mechanisms in two alfalfa cultivars 根际微生物招募和代谢物介导的调节驱动两个苜蓿品种耐盐机制的对比

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2025-12-01 DOI: 10.1016/j.rhisph.2025.101240

Zhuanqiang Wu , Fenfen Liang , Wen Luo , Qian Zhang , Shaowei Li , Hongshan Yang , Changze Han , Yonggang Wang , Zhiqiang Kong , Xiaoli Wang , Xinqiang Zhu

Alfalfa, the “king of forage,” offers high-quality feed and ecological benefits, but salt stress limits its growth and yield. Salt-tolerant alfalfa varieties were selected using ZT1, developed via space-induced mutation breeding, and ZL2, bred through conventional hybridization. By combining metabolomic and 16S rRNA sequencing data from two varieties (ZT1 and ZL2) under gradient NaCl treatments, we uncovered divergent adaptation mechanisms. ZT1 sustained higher photosynthetic performance, achieved better ion balance, and accumulated more flavonoids and terpenoids. It engaged in quorum sensing and beneficial microbial cooperation, adopting an energy-efficient, microbe-assisted strategy to mitigate stress. In contrast, ZL2 relied on broad metabolic reprogramming and enriched microbes linked to defense, demonstrating a more autonomous, energetically costly survival response under high salinity. Microbial diversity decreased with increasing salt stress, but ZT1 maintained a more stable community structure. These findings advance our understanding of plant–microbe–metabolite crosstalk under stress and support the development of breeding or microbiome-based strategies to enhance salt tolerance in Medicago sativa L.

苜蓿，“草料之王”，提供高质量的饲料和生态效益，但盐胁迫限制了它的生长和产量。利用空间诱变选育的ZT1和常规杂交选育的ZL2选育耐盐苜蓿品种。通过结合两个品种（ZT1和ZL2）在梯度NaCl处理下的代谢组学和16S rRNA测序数据，揭示了不同的适应机制。ZT1维持了更高的光合性能，离子平衡更好，积累了更多的黄酮类化合物和萜类化合物。它参与群体感应和有益微生物的合作，采用一种节能的、微生物辅助的策略来减轻压力。相比之下，ZL2依赖于广泛的代谢重编程和与防御相关的丰富微生物，在高盐度下表现出更自主、更消耗能量的生存反应。微生物多样性随着盐胁迫的增加而下降，但ZT1保持了更稳定的群落结构。这些发现促进了我们对胁迫下植物-微生物-代谢物串扰的理解，并为开发基于育种或微生物组的策略来提高紫花苜蓿的耐盐性提供了支持。

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引用次数: 0

Clonal propagation of Mimosa caesalpiniifolia: Effects of phenotype, IBA, and leaf area reduction on rooting and plant propagation 含羞草无性系繁殖：表型、IBA和叶面积减少对植物生根和繁殖的影响

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2025-12-01 DOI: 10.1016/j.rhisph.2025.101232

Alvaro da Costa Freire , Elen Raquel Ferreira Maciel , Françóyse Dávilla de Souza Silva , Jayane Karine Pereira de Araújo , Brayan Paiva Cavalcante , Poliana Coqueiro Dias Araujo

Clonal propagation of Mimosa caesalpiniifolia is essential for multiplying prickleless phenotypes, which enhance silvicultural management and ecological restoration in the Caatinga biome. This study evaluated the effects of plant phenotype (prickly vs. prickleless), indole-3-butyric acid (IBA) concentration, and leaf area reduction on adventitious root formation in mini-cuttings. Three factorial experiments were conducted: (i) evaluation of clonal mini-garden performance across successive prunings; (ii) two phenotypes subjected to three IBA concentrations (0, 2000, and 4000 mg L⁻¹); and (iii) two phenotypes under three levels of leaf area reduction (0 %, 50 %, and 100 %). A randomized block design was adopted, with three replicates and 15 propagules per plot. All mini-stumps survived, with no mortality recorded for either phenotype. Shoot productivity ranged from 156 to 280 shoots/m² across five collections, and analysis of variance indicated no significant interaction between phenotype and collection number (p > 0.05). A significant interaction between phenotype and IBA was observed (p < 0.05). The prickleless phenotype showed the highest survival and rooting rates (≥80 %) with 2000 mg L⁻¹ IBA, while the prickly phenotype rooted best without IBA, declining with higher auxin levels. Leaf area reduction had a marked effect (p < 0.0001): complete defoliation (100 %) severely impaired rooting and survival, whereas moderate reduction (50 %) enhanced physiological balance and rooting performance. In conclusion, mini-cutting propagation is an effective strategy for M. caesalpiniifolia, requiring phenotype-specific protocols. Exogenous IBA improves rooting in prickleless phenotypes, while prickly ones respond better without auxin application. A 50 % leaf reduction increases propagation efficiency and supports scalable clonal production of this species.

含羞草的无性系繁殖是实现无刺表型繁殖的必要条件，可提高Caatinga生物群系的造林管理和生态恢复。本研究评估了植物表型（多刺与无刺）、吲哚-3-丁酸（IBA）浓度和叶面积减少对小插条不定根形成的影响。进行了三因子试验：(1)评价连续修剪对无性系迷你园的影响；（ii）受三种IBA浓度（0,2000和4000 mg L−1）影响的两种表型；3个叶面积减少水平（0 %、50 %和100 %）下的2种表型。采用随机区组设计，3个重复，每个小区15个繁殖体。所有的小树桩都存活了下来，两种表型都没有死亡记录。5个采集区的芽产量在156 ~ 280枝/m2之间，方差分析表明表型与采集量之间无显著交互作用（p >； 0.05）。表型与IBA之间存在显著的交互作用（p <； 0.05）。当添加2000 mg L−1 IBA时，无刺表型的成活率和生根率最高（≥80 %），而不添加IBA时，多刺表型的生根效果最好，随着生长素水平的升高而下降。叶面积减少效果显著（p <； 0.0001）：完全落叶（100 %）严重损害生根和存活，而中度落叶（50 %）增强生理平衡和生根性能。综上所述，小切割繁殖是一种有效的策略，需要表型特异性的方案。外源IBA促进无刺表型的生根，而有刺表型在不施用生长素的情况下反应更好。50% %的叶片减少增加了繁殖效率，并支持该物种可扩展的无性系生产。

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引用次数: 0

Rhizosphere fungi mediated priming reinforces cellular and biochemical defenses against gummy stem blight in muskmelon 根际真菌介导的启动增强了甜瓜对粘茎枯萎病的细胞和生化防御

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2025-12-01 DOI: 10.1016/j.rhisph.2025.101236

Sindhu Geemarahalli Mahadevaswamy , Savitha De Britto , Praveen Satapute , Mostafa Abdelrahman , Lam-Son Phan Tran , Kazunori Sasaki , Kestur Nagaraj Amruthesh , Sudisha Jogaiah

Rhizosphere-associated fungi are recognized as a highly promising and sustainable approach for cultivating high-yielding disease-resistant crops. However, identifying beneficial rhizosphere fungi is a critical step toward achieving optimal protection. In this study, we evaluated 75 rhizosphere-associated fungi isolated from the rhizosphere soil of healthy native muskmelon (Cucumis melo) plants for their in vitro antagonistic activity against Stagonosporopsis cucurbitacearum, the pathogen causing gummy stem blight in muskmelon. Among the 75 isolates, three isolates, Asp-MRF54, Tri-MRF47, and Pen-MRF18, exhibited the highest inhibition rates of 81.9 %, 69.2 %, and 67.6 % respectively, against S. cucurbitacearum mycelial growth. Based on the ITS sequence, the three isolates were identified as Aspergillus niger (Asp-MRF54), Trichoderma virens (Tri-MRF47), and Penicillium italicum (Pen-MRF18). Furthermore, muskmelon seeds primed with Pen-MRF18 and Tri-MRF47 exhibited significantly higher germination rate of 86 % and 81 % and seedling vigor of 1987 and 1955, respectively. Pen-MRF18-primed plants exhibited significant growth improvements, with a 23.8 % increase in chlorophyll content and enhanced nutrient uptake, including nitrogen (33.8 %), phosphorus (42.2 %), and potassium (56.9 %), compared to Tri-MRF47-primed and untreated control plants. Additionally, Pen-MRF18-primed plants demonstrated the highest disease protection of 71.09 % against S. cucurbitacearum, followed by Tri-MRF47-treated plants with 77.5 % protection, compared to non-primed pathogen-inoculated plants. Furthermore, both Pen-MRF18 and Tri-MRF47 treatments induced a significant increase in the accumulation of callose, lignin, phenols, and hydrogen peroxide under both control and pathogen inoculation conditions, indicating an activated cellular defense response. In Pen-MRF18-treated plants challenged with a pathogen, enzymatic activities followed a consistent pattern, with phenylalanine ammonia-lyase and lipoxygenase peaking at 48 h, polyphenol oxidase at 24 h, and peroxidase at 12 h, compared with Tri-MRF47-treated plants. This study demonstrates that Pen-MRF18-primed muskmelon plants exhibit the highest resistance to S. cucurbitacearum infection, followed by those primed with Tri-MRF47, through the activation of integrated cellular, biochemical, and antioxidant defense pathways.

根际相关真菌被认为是培育高产抗病作物的一种非常有前途和可持续的方法。然而，确定有益的根际真菌是实现最佳保护的关键一步。在这项研究中，我们从健康的本土甜瓜根际土壤中分离了75种根际相关真菌，以评估它们对引起甜瓜粘茎枯萎病的病原菌——瓜螺孢子菌（Stagonosporopsis cucurbitacearum）的体外拮抗活性。其中，Asp-MRF54、Tri-MRF47和penn - mrf18对葫芦状葡萄球菌菌丝生长的抑制率最高，分别为81.9 %、69.2% %和67.6% %。经ITS序列鉴定，3株分离物分别为黑曲霉（Asp-MRF54）、绿木霉（Tri-MRF47）和意大利青霉（Pen-MRF18）。在1987年和1955年，用penm - mrf18和Tri-MRF47处理的甜瓜种子发芽率分别为86%和81%，幼苗活力显著提高。与tri - mrf47和未处理对照植株相比，经pen - mrf18处理的植株表现出显著的生长改善，叶绿素含量增加23.8%，氮（33.8%）、磷（42.2%）和钾（56.9%）的养分吸收量增加。此外，与未引物的病原菌接种植株相比，经penmrf18引物处理的植株对S. cucurbitacearum的保护率最高，为71.09%，其次是经tri - mrf47处理的植株，保护率为77.5% %。此外，在对照和病原菌接种条件下，Pen-MRF18和Tri-MRF47处理均诱导胼殖质、木质素、酚类物质和过氧化氢的积累显著增加，表明激活了细胞防御反应。与tri - mrf47处理的植株相比，经peni - mrf18处理的植株受到病原菌胁迫后，酶活性遵循一致的模式，苯丙氨酸解氨酶和脂氧合酶在48 h达到峰值，多酚氧化酶在24 h达到峰值，过氧化物酶在12 h达到峰值。本研究表明，通过激活细胞、生化和抗氧化综合防御途径，以pen - mrf18为引物的甜瓜植株对S. cucurbitacearum感染表现出最高的抗性，其次是Tri-MRF47引物的甜瓜植株。

{"title":"Rhizosphere fungi mediated priming reinforces cellular and biochemical defenses against gummy stem blight in muskmelon","authors":"Sindhu Geemarahalli Mahadevaswamy , Savitha De Britto , Praveen Satapute , Mostafa Abdelrahman , Lam-Son Phan Tran , Kazunori Sasaki , Kestur Nagaraj Amruthesh , Sudisha Jogaiah","doi":"10.1016/j.rhisph.2025.101236","DOIUrl":"10.1016/j.rhisph.2025.101236","url":null,"abstract":"<div><div>Rhizosphere-associated fungi are recognized as a highly promising and sustainable approach for cultivating high-yielding disease-resistant crops. However, identifying beneficial rhizosphere fungi is a critical step toward achieving optimal protection. In this study, we evaluated 75 rhizosphere-associated fungi isolated from the rhizosphere soil of healthy native muskmelon (<em>Cucumis melo</em>) plants for their <em>in vitro</em> antagonistic activity against <em>Stagonosporopsis cucurbitacearum</em>, the pathogen causing gummy stem blight in muskmelon. Among the 75 isolates, three isolates, Asp-MRF54, Tri-MRF47, and Pen-MRF18, exhibited the highest inhibition rates of 81.9 %, 69.2 %, and 67.6 % respectively, against <em>S. cucurbitacearum</em> mycelial growth. Based on the ITS sequence, the three isolates were identified as <em>Aspergillus niger</em> (Asp-MRF54), <em>Trichoderma virens</em> (Tri-MRF47), and <em>Penicillium italicum</em> (Pen-MRF18). Furthermore, muskmelon seeds primed with Pen-MRF18 and Tri-MRF47 exhibited significantly higher germination rate of 86 % and 81 % and seedling vigor of 1987 and 1955, respectively. Pen-MRF18-primed plants exhibited significant growth improvements, with a 23.8 % increase in chlorophyll content and enhanced nutrient uptake, including nitrogen (33.8 %), phosphorus (42.2 %), and potassium (56.9 %), compared to Tri-MRF47-primed and untreated control plants. Additionally, Pen-MRF18-primed plants demonstrated the highest disease protection of 71.09 % against <em>S. cucurbitacearum</em>, followed by Tri-MRF47-treated plants with 77.5 % protection, compared to non-primed pathogen-inoculated plants. Furthermore, both Pen-MRF18 and Tri-MRF47 treatments induced a significant increase in the accumulation of callose, lignin, phenols, and hydrogen peroxide under both control and pathogen inoculation conditions, indicating an activated cellular defense response. In Pen-MRF18-treated plants challenged with a pathogen, enzymatic activities followed a consistent pattern, with phenylalanine ammonia-lyase and lipoxygenase peaking at 48 h, polyphenol oxidase at 24 h, and peroxidase at 12 h, compared with Tri-MRF47-treated plants. This study demonstrates that Pen-MRF18-primed muskmelon plants exhibit the highest resistance to <em>S. cucurbitacearum</em> infection, followed by those primed with Tri-MRF47, through the activation of integrated cellular, biochemical, and antioxidant defense pathways.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101236"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145693517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soil–microbiome–plant interactions mediated by dark septate endophytes and extracellular polymeric substances drive ecological restoration in open-pit dumps 暗隔内生菌和胞外聚合物质介导的土壤-微生物-植物相互作用驱动露天垃圾场生态恢复

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2025-12-01 DOI: 10.1016/j.rhisph.2025.101225

Hai Tan , Yinli Bi , Shishuang Zhang , Jiapeng Kang , Kun Wang , Dongdong Wang

Ecological restoration in arid mining areas is hindered by severely degraded soil structure and disrupted nutrient cycling. Microbial mechanisms regulating rhizosphere processes remain underexplored. This study assessed the seasonal dynamics of rhizosphere microbial biomass and soil nutrients, along with the one-year effects of dark septate endophytes (DSE) and their extracellular polymeric substances (EPS) on microbial diversity, metabolism, and plant growth in open-pit dumps during early construction stages. After one year, the EPS treatment increased MBC, MBN, and MBP by 71.5 %, 54.1 %, and 55.7 %, respectively, compared to the control. Although DSE treatment alone also enhanced MBC, MBN, and MBP, its effects were generally less pronounced than EPS or ‘EPS + DSE’ treatment. Untargeted metabolomics revealed that lipid-derived compounds comprised over 50 % of differential metabolites and their concentration changes were significantly positively correlated with root biomass. Co-occurrence network analysis showed that EPS and ‘EPS + DSE’ treatments increased bacterial–bacterial edges by 19.24 % and 16.9 %, and bacterial–fungal edges by 49.65 % and 14.75 %, respectively, demonstrating a significant increase in microbial network complexity. Plant biomass increased by 2.18-, 1.93-, and 2.78-fold under EPS, DSE, and combined ‘EPS + DSE’ treatments, respectively, compared with the control, while the net photosynthetic rate increased by 1.86-, 1.20-, and 1.70-fold. These results demonstrate that soil–microbiome–plant interactions mediated by DSE and EPS drive ecological restoration in open-pit dumps, providing a targeted microbial approach for restoring degraded mining soils.

干旱矿区土壤结构严重退化，养分循环中断，阻碍了生态恢复。微生物调节根际过程的机制仍未得到充分探索。本研究评估了露天排土场根际微生物生物量和土壤养分的季节动态，以及暗隔内生菌（DSE）及其胞外聚合物（EPS）对露天排土场早期建设阶段微生物多样性、代谢和植物生长的影响。一年后，与对照组相比，EPS处理使MBC、MBN和MBP分别增加了71.5%、54.1%和55.7%。虽然单独DSE治疗也能增强MBC、MBN和MBP，但其效果通常不如EPS或“EPS + DSE”治疗明显。非靶向代谢组学显示，脂质衍生化合物占差异代谢物的50%以上，其浓度变化与根生物量呈显著正相关。共发生网络分析表明，EPS和“EPS + DSE”处理使细菌-细菌边缘分别增加了19.24%和16.9%，细菌-真菌边缘分别增加了49.65%和14.75%，表明微生物网络复杂性显著增加。与对照相比，EPS、DSE和“EPS + DSE”组合处理的植株生物量分别增加了2.18倍、1.93倍和2.78倍，净光合速率分别增加了1.86倍、1.20倍和1.70倍。这些结果表明，DSE和EPS介导的土壤-微生物组-植物相互作用驱动了露天排土场的生态恢复，为恢复退化的矿山土壤提供了有针对性的微生物途径。

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引用次数: 0

Spatiotemporal analysis of root tissue composition indicates soil available Si, NH4+and NO3− predict ginseng nutraceutical quality 根系组织组成的时空分析表明，土壤有效硅、NH4+和NO3−可以预测人参的营养品质

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2025-12-01 DOI: 10.1016/j.rhisph.2025.101233

Zhefeng Xu , Yuqiu Chen , Yibing Wang , Jiahong Sui , Ruixue Yang , Yan Xue , Kemeng Zhang , Jing Fang , Qinghe Zhang , Changbao Chen , Tao Zhang

Ginseng has a wide range of medicinal and edible values, but the specificity of the accumulation of chemical constituents that affect the dual-use value of ginseng is not clear. In this study, We compared 1-year-old (G1), 2-year-old (G2), 3-year-old (G3) and 4-year-old (G4) field-cultivated ginseng to determine specific distribution of chemical constituents and endophytic enzymes in roots and five parts of roots (phloem, xylem, rhizome, lateral roots, and fibrous roots). Meanwhile, the rhizosphere soil properties were determined, and the differences and correlation of these factors were analyzed. We mainly found that the content of soil available silicon, ammonium nitrogen and nitrate nitrogen played a sustained and critical role in the tissue-specificity of ginsenosides. Then, we observed that ribonuclease and laccase in the soil mainly affected the tissue-specific distribution of endophytic enzymes during the first 2 years of ginseng growth. In addition, we found that the tissue-specific distribution of carbohydrate content in root was most influenced by endophytic enzymes. Finally, we constructed an ecological regulation network based on "chemical constituents - endophytic enzymes - soil properties". The results provided a new insight into the tissue-specific distribution of chemical constituents in roots.

人参具有广泛的药用和食用价值，但影响人参军民两用价值的化学成分积累的特异性尚不清楚。在这项研究中，我们比较了1岁（G1）、2岁（G2）、3岁（G3）和4岁（G4）的大田栽培人参，以确定根和根的五个部位（韧皮部、木质部、根茎、侧根和纤维根）的化学成分和内生酶的具体分布。同时测定了根际土壤性状，分析了各因子之间的差异和相关性。主要发现土壤有效硅、铵态氮和硝态氮含量对人参皂苷的组织特异性起着持续而关键的作用。然后，我们观察到土壤中的核糖核酸酶和漆酶主要影响人参生长前2年内生酶的组织特异性分布。此外，我们发现根系碳水化合物含量的组织特异性分布受内生酶的影响最大。最后，构建了基于“化学成分-内生酶-土壤性质”的生态调控网络。该结果为研究根中化学成分的组织特异性分布提供了新的视角。

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引用次数: 0

Rhizobacteria of native aquatic macrophytes in coal mining subsidence ponds are shaped by compartment niche differentiation 煤矿沉陷塘原生水生植物的根菌群是由隔间生态位分化形成的

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2025-11-19 DOI: 10.1016/j.rhisph.2025.101235

Ting Gao , Songbao Feng , Sanping Yu , Xianyang Shi , Jin Cheng

Aquatic macrophytes have evolved adaptive mechanisms to thrive in the heterogeneous environments of coal mining subsidence areas. While such mechanisms provide practical solutions for ecological restoration in mining areas, general principles driving community assembly and the potential functions of bacterial microbiotas associated with these plants remain poorly characterized. In this study, we employed Illumina-based sequencing approaches to systematically characterize the bacterial microbiota in two root-associated compartments (rhizosphere and root endosphere) of four dominant macrophyte species (common reed, narrow-leaf cattail, short-lived flatsedge, and tuber bulrush) colonizing contrasting substrates (sediment and coal gangue). Our analyses revealed that the characteristics of root-associated bacterial communities were determined primarily by root compartment rather than by macrophyte species or plant habitat. Rhizospheric and root endophytic communities differed significantly in composition, network complexity, and keystone species, confirming a compartmentalized niche-assembly pattern. Functional predictions demonstrated metabolic niche partitioning between compartments, with distinct biogeochemical pathways enriched in each microenvironment. Specifically, rhizosphere communities were enriched in anaerobic respiration, nitrogen cycling, lignin degradation, and anoxygenic photosynthesis; meanwhile, endospheric microbiota were enriched in dark hydrogen oxidation, ureolysis, methylotrophy, and fumarate respiration. This study sheds new light on the assembly and functional roles of root-associated bacterial communities in coal mining subsidence ponds, supporting future ecological restoration efforts by identifying key functional bacteria.

水生植物已经进化出适应机制，在煤矿沉陷区异质环境中茁壮成长。虽然这些机制为矿区的生态恢复提供了切实可行的解决方案，但驱动群落组装的一般原理以及与这些植物相关的细菌微生物群的潜在功能仍不清楚。在这项研究中，我们采用基于illumina的测序方法，系统地表征了四种优势大型植物物种（芦苇、窄叶香蒲、短寿扁草和块茎芦苇）在两个根相关隔间（根际和根内圈）中定殖在不同基质（沉积物和煤矸石）中的细菌微生物群。我们的分析表明，根相关细菌群落的特征主要由根室决定，而不是由大型植物种类或植物栖息地决定。根际和根内生群落在组成、网络复杂性和关键物种方面存在显著差异，证实了分区的生态位组合模式。功能预测表明，在每个微环境中，不同的生物地球化学途径丰富的隔间之间的代谢生态位分配。具体而言，根际群落在厌氧呼吸、氮循环、木质素降解和无氧光合作用中富集；同时，在暗氢氧化、尿素溶解、甲基化和富马酸呼吸过程中，内圈微生物群富集。该研究揭示了煤矿沉陷池中与根相关的细菌群落的组成和功能作用，通过识别关键功能细菌，为未来的生态修复工作提供支持。

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引用次数: 0

Rice husk-derived nanosilica enhances root development, phosphorus uptake and soil stability in rice paddy fields of northern Iran 稻壳衍生纳米二氧化硅促进了伊朗北部稻田根系发育、磷吸收和土壤稳定性

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2025-11-17 DOI: 10.1016/j.rhisph.2025.101234

Misagh Parhizkar

This study evaluates the impact of rice husk–derived nanosilica on root morphology, water and phosphorus uptake, and soil detachment dynamics in paddy fields of northern Iran. Nanosilica was synthesized via acid leaching and pyrolysis and characterized by FTIR and FESEM, confirming its amorphous structure and uniform morphology (mean size ≈15 nm). Field flume and plot experiments demonstrated that nanosilica application significantly enhanced root length, biomass, and diameter while increasing root moisture and phosphorus uptake (p < 0.01). In contrast, soil detachment capacity decreased by 1.52-fold, and aggregate stability improved by 1.33-fold relative to control soils. The hydrophilic and reactive surface of nanosilica promoted interparticle bonding and water retention, resulting in improved soil structure and plant performance. Principal component analysis revealed distinct separation between treated and untreated soils, driven by enhanced root traits and stability indicators. Overall, rice husk–derived nanosilica proved to be a sustainable and eco-friendly nanomaterial that improves soil resilience and crop efficiency in flooded rice ecosystems.

本研究评估了稻壳衍生纳米二氧化硅对伊朗北部稻田根系形态、水磷吸收和土壤分离动力学的影响。采用酸浸和热解法制备了纳米二氧化硅，并用FTIR和FESEM对其进行了表征，证实了纳米二氧化硅的无定形结构和均匀形貌（平均粒径≈15 nm）。田间水槽和地块试验表明，施用纳米二氧化硅显著增加了根系长度、生物量和直径，同时增加了根系对水分和磷的吸收（p <； 0.01）。与对照土相比，土壤剥离能力降低了1.52倍，团聚体稳定性提高了1.33倍。纳米二氧化硅的亲水性和活性表面促进了颗粒间的结合和保水，从而改善了土壤结构和植物性能。主成分分析结果表明，处理土壤与未处理土壤之间存在明显的差异，这主要受根系性状和稳定性指标增强的驱动。总之，稻壳衍生的纳米二氧化硅被证明是一种可持续和生态友好的纳米材料，可以提高水淹水稻生态系统中的土壤恢复力和作物效率。

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引用次数: 0

Patterns of rhizosphere microbial community assembly affect blueberry growth: environmental and host effects 根际微生物群落组合模式对蓝莓生长的影响：环境和宿主效应

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2025-11-15 DOI: 10.1016/j.rhisph.2025.101228

Chunhuan Li , Ning Jiao , Siyu Liu , Laiye Qu , Ying Zhang , Naili Zhang

Blueberries, among the top five healthiest fruits globally, thrive in acidic soils being closely linked to the rhizosphere microbiome. Different blueberry cultivars harbor distinct rhizosphere microbial communities, which may reflect their unique adaptive strategies to acidic soil conditions. To investigate the relationship, we conducted a field experiment, with three acidic soil types (strongly acidic, moderately acidic, and weakly acidic) and three blueberry cultivars (Southern Highbush, Northern Highbush, and Rabbiteye Blueberries). To elucidate the microbial-mediated adaptation of different blueberry cultivars to acidic soils, we focused on the composition, diversity and network structure of rhizosphere bacterial and fungal communities. A total of 3,610,964 bacterial operational taxonomic units (OTUs) and 4,307,278 fungal OTUs were obtained. Our findings showed that the rhizosphere bacterial community was significantly more diverse under strongly acid conditions than under weakly acid conditions, whereas no such difference was observed in the fungal communities. Although the microbial communities associated with different blueberry cultivars exhibited high compositional similarity, significant differences were observed in network complexity and the recruitment of keystone taxa of both bacterial and fungal communities. Furthermore, structural equation modeling revealed that the network topological characteristics of bacterial (path coefficient = 0.382, P < 0.05) and fungal (path coefficient = −0.453, P < 0.01) communities had a significant effect on the basal diameter of blueberries, explaining 55.9 % and 72.3 % of its variance, respectively. These results highlight the significance of the structure, rather than simply the diversity, of rhizosphere microbial communities in regulating plant growth and adaptation. This statement emphasizes the critical role of microbial network structure in plant adaptation and its potential application in agriculture.

蓝莓是全球五大最健康的水果之一，在酸性土壤中茁壮成长，与根际微生物群密切相关。不同蓝莓品种的根际微生物群落差异较大，这可能反映了其对酸性土壤条件的独特适应策略。为了研究这一关系，我们在3种酸性土壤类型（强酸性、中酸性和弱酸性）和3种蓝莓品种（南方高丛、北方高丛和兔眼蓝莓）上进行了田间试验。为了阐明不同蓝莓品种对酸性土壤的微生物适应性，我们重点研究了蓝莓根际细菌和真菌群落的组成、多样性和网络结构。共获得3610,964个细菌操作分类单位（otu）和4,307,278个真菌操作分类单位（otu）。结果表明，在强酸条件下，根际细菌群落的多样性明显高于弱酸条件，而真菌群落的多样性没有明显差异。虽然不同蓝莓品种的微生物群落具有较高的相似性，但在细菌和真菌群落的网络复杂性和关键类群的招募方面存在显著差异。此外,结构方程建模显示网络拓扑特征的细菌(路径系数 = 0.382,P & lt; 0.05)和真菌(路径系数 = −0.453,P & lt; 0.01)社区对蓝莓的基底直径有显著的影响,解释 % 55.9和72.3 %的差异,分别。这些结果强调了根际微生物群落的结构，而不仅仅是多样性，在调节植物生长和适应方面的重要性。该声明强调了微生物网络结构在植物适应中的关键作用及其在农业中的潜在应用。

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引用次数: 0

Rhizosphere drives microbial necromass carbon accumulation via enhanced carbon use efficiency: Evidence from a common garden experiment 根际通过提高碳利用效率驱动微生物坏死团碳积累：来自普通园林试验的证据

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere

Pub Date : 2025-11-13 DOI: 10.1016/j.rhisph.2025.101231

Beibei Wang , Kun Yan , Chao Ji , Kun Li , Yanping Wang , Qitong Wang

Soil carbon (C) sequestration is a core issue in global C cycle research, and the rhizosphere, as a hotspot of plant-soil-microbe interactions, still requires in-depth analysis of its mechanism in the formation and stabilization of soil C. This study, based on the "microbial C pump" theory, focuses on the differences in microbial C transformation between the rhizosphere and bulk soils, aiming to reveal how microbial metabolic efficiency regulate the differences in soil C sequestration derived from microbial necromss C between these two soil compartments. We performs a common garden experiment, by measuring amino sugars, systematically compared the differences in the contribution of microbial necromass C to soil organic carbon (SOC) in the rhizosphere of ten tree species and bulk soils. Simultaneously, the ¹⁸O-H₂O labeling technique was used to determine microbial carbon use efficiency (CUE), and the intrinsic relationship between CUE and microbial necromass C accumulation was analyzed. The results showed: (1) The microbial necromass C content in the rhizosphere was significantly higher than that in the bulk soil, increasing by an average of 35.3 %; (2) The contribution of microbial necromass C to SOC in the rhizosphere was 69.1 % higher than that in the bulk soil; (3) The average microbial CUE in the rhizosphere was 192.6 % higher than that in the bulk soil, and was significantly positively correlated with the content of necromass C in the rhizosphere and/or bulk soil. These results consistently indicate that the rhizosphere promotes the accumulation of microbial-derived C through enhancing microbial CUE. Our study mechanistically confirmed the key role of the rhizosphere for soil C sequestration, not only deepening the understanding of the interaction between plants and microorganisms driving the soil C cycle, but also providing scientific evidence for regulating rhizosphere processes to enhance the C sequestration capacity of ecosystems.

土壤碳(C)固存是全球碳循环研究的核心问题，而根际作为植物-土壤-微生物相互作用的热点，其在土壤C形成和稳定中的机制仍需深入分析。本研究基于“微生物C泵”理论，重点研究根际与块状土壤微生物C转化的差异。旨在揭示微生物代谢效率如何调节这两个土壤区室之间因微生物坏死而产生的土壤碳固存差异。本研究采用普通园林试验，通过测定氨基糖，系统比较了10种树种和块状土壤根际微生物坏死块C对土壤有机碳（SOC）贡献的差异。同时，采用18O-H2O标记技术测定微生物碳利用效率（CUE），并分析了CUE与微生物坏死块C积累的内在关系。结果表明：(1)根际微生物坏死体C含量显著高于体土，平均增加35.3% %；(2)根际微生物坏死物C对有机碳的贡献比体土高69.1 %；(3)根际微生物CUE均值比体土高192.6 %，且与根际和（或）体土坏死块C含量呈显著正相关。这些结果一致表明，根际通过增强微生物CUE促进微生物源性碳的积累。本研究从机制上证实了根际在土壤碳固存中的关键作用，不仅加深了对植物与微生物相互作用驱动土壤碳循环的认识，也为调控根际过程增强生态系统碳固存能力提供了科学依据。

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引用次数: 0