Geography is a well-known factor shaping genetic variation in human populations. However, the potential role played by cultural variables remains much understudied. This study investigates the impact of socio-cultural variables on genomic similarity and the saliva microbiome, using data from populations in Lesotho and Namibia. Geographic distance within Lesotho increases genetic differentiation, while shared clan affiliation surprisingly increases it. In Namibia, ethnicity is the predominant factor influencing genetic affinity. Saliva metagenomic data shows a negative correlation between age and alpha diversity, with notable differences in host-interacting taxa and viral load. These findings highlight the role of geography in shaping genetic affinity even at small scales and the complex influences of cultural factors. The saliva microbiome appears primarily affected by unrecorded individual behaviors rather than geographic or cultural variables. At population-level these oral microbiomes reveal insights into some dietary habits, oral health, and also the communal viral load, which appears to have greater incidence in Lesotho possibly related to the long-term effects of the HIV epidemic in the country.
Genetic variability of Roma population was shaped by the strong influence of genetic drift and gene flow during the migrations from their ancestral homeland in Indian subcontinent towards Europe. In addition, social stigmatization in many European countries, as a consequence of different cultural heritage and social practices, induced further genetic differentiation and sub structuring within the population. Although many populations genetic studies on European Roma were carried out, the genetic structure of the Serbian Roma has not been described yet, since only the modest number of individuals from this territory was analyzed. The main aim of this study was the characterization of genetic variability of the Roma and the assessment of intrapopulation genetic differentiation based on the analysis of 21 autosomal STR loci of 259 self-identified unrelated individuals from Serbia. Intrapopulation analysis revealed divergence of Roma groups illustrating the effect of the historical events after their arrival on Balkan Peninsula and emphasizing significance of the religious affiliation on admixture with autochthonous population. Genetic distance analysis showed the greatest similarity of the studied population with the Middle Eastern populations, while South Asian and European population were more distant. Our results demonstrate that Roma groups in this region of Balkan Peninsula do not represent completely isolated, but rather admixed populations with different proportion of gene flow with other Roma and non-Roma groups.

