Brazilian Journal of Otorhinolaryngology最新文献

{"title":"Metabolic biomarkers mediate allergic rhinitis via circulating inflammatory proteins: Evidence from a Mendelian randomization study","authors":"Xiang Cao ,&nbsp;Boyang Zhang ,&nbsp;Wei Wang ,&nbsp;Zijiao Xu ,&nbsp;Zhixin Jiang","doi":"10.1016/j.bjorl.2025.101658","DOIUrl":"10.1016/j.bjorl.2025.101658","url":null,"abstract":"<div><h3>Objective</h3><div>Allergic Rhinitis (AR), a prevalent allergic condition, markedly diminishes quality of life and increases public health burdens. This study investigated the mediating roles of Metabolic Biomarkers (MBs) in the associations between Circulating Inflammatory Proteins (CIPs) and AR, aiming to uncover novel therapeutic targets.</div></div><div><h3>Methods</h3><div>Utilizing Mendelian Randomization (MR), we leveraged genetic variants as instrumental variables to delineate causal relationships between CIPs and AR. Data were extracted from comprehensive Genome-Wide Association Studies (GWAS). A two-sample MR approach was employed to assess both the direct and mediated effects of CIPs on AR through MBs. Inverse-Variance Weighting (IVW) served as the main analytical method, supplemented by rigorous sensitivity analyses to confirm the robustness of the findings.</div></div><div><h3>Results</h3><div>Our analysis revealed that specific CIPs like C-C motif Chemokine-19 (CCL19), Notch-like Epidermal growth factor-related Receptor (DNER), and Interleukin-6 (IL-6) significantly elevate the risk of AR, whereas Interleukin-10 (IL-10) exhibit protective properties. Key MBs, notably Adenosine 5'-Monophosphate (AMP) to urate ratio, plasma lactate, and N-acetylputrescine, played critical roles in mediating these relationships, indicating their potential as therapeutic targets.</div></div><div><h3>Conclusion</h3><div>This investigation highlights the pivotal role of MBs in modulating the influence of CIPs on AR. The insights gained from this study not only deepen our understanding of AR pathophysiology but also open new avenues for metabolic intervention in its treatment.</div></div><div><h3>Level of evidence</h3><div>In terms of level of evidence, MR studies are second only to randomized controlled trials.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101658"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive factors for outcomes of sialoendoscopy","authors":"Hannah Damasceno Barreto ,&nbsp;Jose Higino Steck ,&nbsp;Samuel Serpa Steck ,&nbsp;Diogo Silva de Carvalho ,&nbsp;Carlos Takahiro Chone","doi":"10.1016/j.bjorl.2025.101631","DOIUrl":"10.1016/j.bjorl.2025.101631","url":null,"abstract":"<div><h3>Objectives</h3><div>We evaluated the epidemiological and clinical factors related to the complications and failure of sialoendoscopy.</div></div><div><h3>Methods</h3><div>This retrospective study analyzed the medical records of patients who underwent sialoendoscopy between 2010 and 2019.</div></div><div><h3>Results</h3><div>Of the 174 sialoendoscopies analyzed, 125 (71.8%) were performed in women, and 114 (65.5%), in the parotid gland. The most prevalent complications described were false path (4.59%) and restenosis (2.30%). Complications in the submandibular gland were 3.43 times more common than those in the parotid gland and procedures in that gland were less successful (86.7% vs. 91.2%). Sialolithiasis treatment was most strongly associated with complications and failure then the other groups.</div></div><div><h3>Conclusion</h3><div>Sialoendoscopy has uncommon and limited complications. Procedures in the submandibular gland and for sialolithiasis treatment were more likely to result in complications and recurrences.</div></div><div><h3>Level of evidence</h3><div>4.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101631"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disulfidptosis ‒ related lncRNAs are biomarkers of prognosis and immune response in Head and Neck Squamous Cell Carcinoma","authors":"Ruilin Wang ,&nbsp;Qi Zhang ,&nbsp;Yuxiu Ma ,&nbsp;Xuelin Liu ,&nbsp;Tian Lan ,&nbsp;Hongling Li","doi":"10.1016/j.bjorl.2025.101625","DOIUrl":"10.1016/j.bjorl.2025.101625","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to explore the role of Disulfidptosis-Related long Non-Coding RNAs (DRlncRNAs) in the prognosis and immune infiltration of Head and Neck Squamous Cell Carcinoma (HNSCC).</div></div><div><h3>Methods</h3><div>Using bioinformatics approaches, this study investigates the prognostic significance of DRlncRNAs in HNSCC patients and their potential association with the immune microenvironment. RNA sequencing data and clinical information for HNSCC were obtained from The Cancer Genome Atlas (TCGA) database. DRlncRNAs were identified through Pearson correlation analysis, and a prognostic model consisting of six DRlncRNAs was constructed using Least Absolute Shrinkage and Selection Operator (LASSO) regression, along with univariate and multivariate Cox analyses.</div></div><div><h3>Results</h3><div>The predictive performance of the model was assessed using Receiver Operating Characteristic (ROC) curves and Principal Component Analysis (PCA), and further validated using calibration curves, a nomogram, and univariate/multivariate Cox analyses. In addition to functional enrichment analysis, the associations between the model and Tumor Mutation Burden (TMB), immune cell infiltration, and drug sensitivity were also examined.</div></div><div><h3>Conclusion</h3><div>We developed a novel predictive model composed of six DRlncRNAs to predict the prognosis of HNSCC patients and proposed potential clinical therapeutic targets from the perspective of disulfidptosis.</div></div><div><h3>Level of evidence</h3><div>Level 5.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101625"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphometric evaluation of cerebral and cerebellar structures in long-term unilateral sensorineural hearing loss","authors":"Gözde Orhan Kubat ,&nbsp;Özkan Özen ,&nbsp;Emre Çolak","doi":"10.1016/j.bjorl.2025.101635","DOIUrl":"10.1016/j.bjorl.2025.101635","url":null,"abstract":"<div><h3>Objective</h3><div>Structural changes in the brain have been identified in patients with hearing loss. However, the effect of long-term Unilateral Sensorineural Hearing Loss (USNHL) on cerebral and cerebellar morphology is not fully understood.</div></div><div><h3>Methods</h3><div>In this study, Magnetic Resonance Imaging (MRI) of 12 long-term USNHL adults was compared with their Normal-Hearing (NH) side using Voxel-Based Morphometry (VBM). The USNHL group was also compared to a control group of 12 hearing-healthy individuals. Volume and cortical thickness changes in cerebral and cerebellar structures, possibly related to hearing loss severity and duration, were assessed using VBM.</div></div><div><h3>Results</h3><div>The study found that, compared to the control group, USNHL patients had significantly higher measurements in the Frontal Operculum (FO) Gray Matter (GM) volume (cm³) (<em>p</em> = 0.024), Superior Temporal Gyrus (STG) thickness (<em>p</em> = 0.041), and cerebellum IX GM volume (cm³) (<em>p</em> = 0.025). No significant differences were observed between the intact and NH sides in the USNHL group (<em>p</em> &gt; 0.05).</div></div><div><h3>Conclusion</h3><div>Cerebral and cerebellar structures, which are essential for perceiving and interpreting sound, integrating information, and coordinating movement, may undergo morphological changes due to neuroadaptive mechanisms in long-term USNHL patients. Larger case series are needed to explore the clinical implications of these changes for diagnosis, treatment, and prognosis.</div></div><div><h3>Levels of evidence</h3><div>Level 3.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101635"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel and effective surgical procedure for posterior nasal neurectomy in patients with allergic rhinitis","authors":"Weini Hu ,&nbsp;Qiang Zuo ,&nbsp;Dawei Wu ,&nbsp;Yu Song","doi":"10.1016/j.bjorl.2025.101653","DOIUrl":"10.1016/j.bjorl.2025.101653","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to analyze how to perform a more minimally invasive and precise excision of posterior nasal neurectomy to reduce surgical time, minimize surgical trauma, and evaluate the clinical efficacy of this procedure in the treatment of moderate to severe persistent allergic rhinitis.</div></div><div><h3>Methods</h3><div>This study included a total of 110 patients, randomly divided into an experimental group and a control group based on whether preoperative CT three-dimensional positioning was used and whether middle turbinate mucosal graftcoverage was applied during surgery. In the experimental group, preoperative CT measurements were used to locate the sphenopalatine foramen, and intraoperatively, the accuracy of the positioning method was verified. Middle turbinate mucosal graftcoverage was applied to shorten the postoperative recovery time. Both groups were evaluated using Visual Analog Scales (VAS) and Rhinoconjunctivitis Quality of Life Questionnaire (RQLQ) to compare the differences in symptoms between baseline and six months after operation. Regular endoscopic examinations were conducted to observe the status of mucosal epithelialization at the surgical site.</div></div><div><h3>Results</h3><div>Preoperative CT measurements provided accurate intraoperative localization of the sphenopalatine foramen, allowing for a smaller surgical wound size. The average distance from the sphenopalatine foramen to the posteroinferior end of the uncinate process was 20.25 ± 2.69 mm, and from the sphenopalatine foramen to the upper edge of the inferior turbinate, it was 11.05 ± 1.71 mm. There was no statistically significant difference in preoperative symptom scores between the two groups (<em>p</em> &gt; 0.05). At six months after operation, there were significant differences in VAS scores compared to baseline in both the experimental and control groups (<em>p</em> &lt; 0.05). The average time for mucosal graftsurvival in the experimental group was 3.5 ± 1.0 weeks, while in the control group, the average time for exposed bone surface epithelialization was 9.0 ± 1.5 weeks.</div></div><div><h3>Conclusion</h3><div>Preoperative CT measurements for locating the sphenopalatine foramen and the use of middle turbinate mucosal grafts in the posterior nasal neurectomy are safe and effective. This new procedure reduces surgical wound size, shorten surgical time, accelerate postoperative wound recovery, and could possibly reduce complications of bleeding after surgery.</div></div><div><h3>Level of evidence</h3><div>Level 3.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101653"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends and future directions in chronic rhinosinusitis with nasal polyps: A bibliometric analysis","authors":"Yeming Zhong ,&nbsp;Xuan Wei ,&nbsp;Caiyun Zou ,&nbsp;Bo Qian ,&nbsp;Hongbo Ji ,&nbsp;Jinhe Guo ,&nbsp;Zigang Che","doi":"10.1016/j.bjorl.2025.101672","DOIUrl":"10.1016/j.bjorl.2025.101672","url":null,"abstract":"<div><h3>Objectives</h3><div>Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) is a complex inflammatory condition that significantly impairs patients’ quality of life. Despite significant efforts to develop effective treatments, a comprehensive bibliometric analysis in this field is lacking. This study aims to identify key trends, influential publications, leading authors, and collaborative networks.</div></div><div><h3>Methods</h3><div>A bibliometric analysis was conducted on October 16, 2024, examining publications in the Web of Science Core Collection (WoSCC) database from 2004 to 2024. Data visualization and interpretation were performed using VOSviewer (version 1.6.20), CiteSpace (version 6.3.R1), and R 4.3.3.</div></div><div><h3>Results</h3><div>The analysis included 1,235 publications from 5,825 authors across 4,723 institutions in 222 countries. The United States led in publication volume, followed by China and Italy. Major institutional contributors included Capital Medical University and Sanofi. The International Forum of Allergy &amp; Rhinology published the most articles, while the Journal of Allergy and Clinical Immunology had the highest citation count. Key authors identified were Bachert Claus and Gevaert Philippe. Keyword analysis revealed “asthma”, “endoscopic sinus surgery”, and “inflammation” as dominant themes, with burst analysis indicating growing interest in “humanization”, “type 2 inflammation”, “risk”, “biologics”, and “safety”.</div></div><div><h3>Conclusion</h3><div>This bibliometric analysis underscores an increasing focus on biologic therapies, inflammation management, and endoscopic sinus surgery in CRSwNP research. Future investigations are likely to prioritize the long-term safety and cost-effectiveness of biologics, alongside advancements in precision medicine and surgical techniques.</div></div><div><h3>Level of evidence</h3><div>Level 1.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101672"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes in patients with head and neck squamous cell carcinoma with exclusively surgical resection","authors":"Daniel Naves Araujo Teixeira ,&nbsp;Fabio Lau ,&nbsp;Vanessa Carvalho de Oliveira ,&nbsp;Eduardo Vieira Couto ,&nbsp;Thomas Peter Maahs ,&nbsp;Carmen Silvia Passos Lima ,&nbsp;Carlos Takahiro Chone","doi":"10.1016/j.bjorl.2025.101622","DOIUrl":"10.1016/j.bjorl.2025.101622","url":null,"abstract":"<div><h3>Objectives</h3><div>To estimate patients’ overall and disease-free survival with squamous cell carcinoma of the oral cavity, oropharynx, and larynx, and assess the influence of primary local staging and histopathological features on these outcomes.</div></div><div><h3>Methods</h3><div>Retrospective data analysis of 102 patients with head and neck squamous cell carcinoma who underwent exclusive surgery as the initial treatment modality. p16 analysis was not evaluated. Exclusion criteria included patients with synchronous or metachronous tumors, those undergoing adjuvant or neoadjuvant chemotherapy or radiotherapy, and those with prior surgery at the exact site. Univariate and multivariate Cox Regression analyses were performed to evaluate outcome-related factors. Survival curves were estimated using the Kaplan-Meier method.</div></div><div><h3>Results</h3><div>The majority presented early stage I and II (83.4%), T1/T2 tumors (86.2%), N0 (95.1%), and all M0. The primary sites were mainly the larynx (46.1%) and oral cavity (41.2%). The mean overall survival was 71.76 months, with survival rates at 1-, 2-, 3-, 4-, and 5-years of 95.9%, 88.2%, 85.5%, 80.4%, and 74.7%, respectively. The mean disease-free survival was 68.2 months, with rates at 1-, 2-, 3-, 4-, and 5-years of 87.5%, 85.1%, 82.2%, 76.6%, and 69.3%, respectively. Active smoking was associated with a higher risk of death (HR = 9.4, <em>p</em> &lt;  0.001) and recurrence (HR = 9.7, <em>p</em> &lt;  0.001). Active smoking patients with perineural invasion presented worse overall and disease-free survival. Positive margins were associated with reduced disease-free survival.</div></div><div><h3>Conclusion</h3><div>Exclusive surgery in the early stages (I and II) emerges as a practical approach. Stage, primary site, margins, perineural invasion, lymphovascular invasion, and smoking cessation were the significant prognostic factors that affected patient outcomes.</div></div><div><h3>Level of evidence</h3><div>2C.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101622"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Voice symptoms, dysphagia risk, quality of life, and mental health in total laryngectomized patients: a self-perception study","authors":"João Vitor Barbosa Pereira ,&nbsp;Vaneli Colombo Rossi ,&nbsp;Carlos Takahiro Chone ,&nbsp;Ana Carolina Constantini","doi":"10.1016/j.bjorl.2025.101657","DOIUrl":"10.1016/j.bjorl.2025.101657","url":null,"abstract":"<div><h3>Objectives</h3><div>To establish the sociodemographic profile, identify and relate the presence of voice symptoms, risk for dysphagia, and self-perceived emotional distress, and measure the quality of life of a group of total laryngectomized patients.</div></div><div><h3>Method</h3><div>This is a descriptive, quantitative, cross-sectional study. Subjects aged 18-years and older, of both sexes, who had undergone total laryngectomy. The sample composition was non-probabilistic. A questionnaire was used to obtain sociodemographic data. The perception of voice symptoms was assessed by the Voice Symptom Scale (VoiSS) and the risk of dysphagia was screened using the Eating Assessment Tool (EAT-10). Mental health aspects were measured using the Self-Reporting Questionnaire (SRQ-20) and data on general health, treatment and illness were collected using the European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire Core-30 (EORTC QLQ-C30).</div></div><div><h3>Results</h3><div>12 participants were recruited; mostly male, average age of 64-years. All participants presented voice symptoms and 33% were screened with self-perceived emotional distress and 66.7% progressed with risk for dysphagia; all of them had undergone at least radiotherapy during the treatment.</div></div><div><h3>Conclusion</h3><div>All subjects in the sample had voice symptoms. Risk of dysphagia emerged as a determining factor in the quality of life and mental health of study participants, and was also related to concomitant voice symptoms. In this sample, participants with a high prevalence of voice signs and symptoms were at greater risk of emotional distress.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101657"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term efficacy and safety of different biologics in treatment of chronic rhinosinusitis with nasal polyps: A network meta-analysis","authors":"Han Chen ,&nbsp;Lin Wang ,&nbsp;Jisheng Zhang,&nbsp;Xudong Yan,&nbsp;Longgang Yu,&nbsp;Yan Jiang","doi":"10.1016/j.bjorl.2025.101633","DOIUrl":"10.1016/j.bjorl.2025.101633","url":null,"abstract":"<div><h3>Objectives</h3><div>Direct comparison of the long-term effectiveness and safety of biologics for Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) is lacking. The study aimed to compare the long-term efficacy and safety of various biologics in the management of CRSwNP.</div></div><div><h3>Methods</h3><div>The PubMed, Embase, Cochrane Library, and Web of Science databases were searched from database inception to March 2024, to identify all relevant literature on the use of biologics for CRSwNP. The research protocol was registered on PROSPERO.Two independent reviewers screened the studies, extracted the data, and performed a quality assessment using the Cochrane Risk of Bias tool. Network meta-analysis was conducted using STATA 17.0 and Review Manager (Version 5.3).</div></div><div><h3>Results</h3><div>Six studies with a minimum follow-up period of 52-weeks were analyzed, demonstrating the superior long-term efficacy of dupilumab compared to the other three biologics. The surface under the cumulative ranking curve were 100% for Nasal Polyp Score (NPS), 72.7% for Sino-Nasal Outcome Test-22 (SNOT-22), 94.6% for Visual Analogue Scale (VAS), and 100% for Nasal Congestion Score (NCS). In the comparison of NPS, dupilumab showed improvements of 1.84 (95% Confidence Interval [95% CI 0.78, 2.91]) over mepolizumab and 2.31 (95% CI 0.99, 3.63) over benralizumab. Among the symptom scores evaluated, only dupilumab achieved a significant improvement in NCS compared to benralizumab, with an improvement of 0.74 (95% CI 0.86, 1.19). No significant differences in adverse events was observed between biologics treatment or versus placebo.</div></div><div><h3>Conclusion</h3><div>Treatment with dupilumab in patients with CRSwNP has shown superior long-term efficacy in reducing NPS and various symptom scores compared to other biologics. However, the results should be interpreted with caution due to the heterogeneity of patient characteristics across the included studies (e.g., disease severity, history of surgery, use of oral corticosteroids).</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 4","pages":"Article 101633"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of key hub genes and potential therapeutic drugs for nasopharyngeal carcinoma: Insights into molecular mechanisms and treatment strategies","authors":"Haiyan Quan ,&nbsp;Hongguo Yin ,&nbsp;Zhen Wang ,&nbsp;Yuan Lv ,&nbsp;Qiong Sun ,&nbsp;Ting Yin","doi":"10.1016/j.bjorl.2025.101618","DOIUrl":"10.1016/j.bjorl.2025.101618","url":null,"abstract":"<div><h3>Objective</h3><div>Nasopharyngeal Carcinoma (NPC) is a highly malignant cancer with a high incidence in East and Southeast Asia, including southern China. Despite advances in treatment, the prognosis for advanced NPC remains poor due to high recurrence and metastasis rates. The molecular mechanisms driving NPC progression are not fully understood, and identifying key genes and potential therapeutic agents is critical. This study aims to uncover critical genes and screen therapeutic drugs, providing insights into NPC pathogenesis and novel treatment strategies.</div></div><div><h3>Methods</h3><div>Three GEO datasets (GSE12452, GSE53819, and GSE61218) were analyzed to identify overlapping Differentially Expressed Genes (DEGs) in NPC. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) were used to explore the biological roles of DEGs. Protein-Protein Interaction (PPI) and mRNA-miRNA-lncRNA interaction networks were constructed to identify key hub genes. Potential therapeutic drugs were predicted via a Drug-Gene Interaction network. The overexpression of hub genes was validated in NPC cells using CCK-8 assays, and the anti-proliferative effects of three drugs ‒ valproic acid, cyclosporine, and calcitriol ‒ were tested.</div></div><div><h3>Results</h3><div>Eight hub genes (ASPM, BIRC5, BUB1B, CDK1, KIF23, PBK, TOP2A, and TTK) were identified, with ASPM reported for the first time in the context of NPC. Overexpression of these genes significantly promoted NPC cell proliferation. Among the tested drugs, calcitriol exhibited the most potent anti-proliferative effect, with IC50 values of 0.90 μM, 0.47 μM, and 0.31 μM at 24-, 48-, and 72-hs, respectively.</div></div><div><h3>Conclusion</h3><div>This study identified eight key genes as potential biomarkers for NPC and validated calcitriol as a promising therapeutic agent, providing a foundation for further research into NPC treatment.</div></div><div><h3>Level of evidence</h3><div>Level 2 (Individual cross-sectional studies or systematic review of surveys that allow matching to local circumstances).</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 4","pages":"Article 101618"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}