Brazilian Journal of Otorhinolaryngology最新文献_第7页

Functional outcomes of otosclerosis surgery 耳硬化手术的功能结局

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Brazilian Journal of Otorhinolaryngology

Pub Date : 2025-07-11 DOI: 10.1016/j.bjorl.2025.101679

Nadia Romdhane, Dorra Chiboub, Amal Nessib, Ameni Amri, Ines Hariga, Chiraz Chaouch Mbarek

Objectives

The objectives of this work were to evaluate the results of otosclerosis surgery and to study the predictors of its functional success.

Methods

Our study was retrospective on 322 patients, equivalent to 418 ears operated in the otolaryngology and head and neck surgery department, from January 2000 to December 2020.

Results

Hearing success was noted in 89.5% of patients considering the Postoperative Residual Air Bone Gap (PRABG) ≤10 dB criterion; in 94.3% of patients according to the conductive gain ≥20 dB criterion; in 96.4% of patients based on the improvement of cochlear reserve criterion; in 88.8% according to the Air Bone Gap improvement (ABGi) ≥70% criterion and in 81.8% considering all these criteria. The short, medium and long-term postoperative tonal audiometries could be superimposed, reflecting the stability of the hearing results. A regression of tinnitus has been reported in 79.2% of cases. The independent predictors of hearing success were the Aubry's audiometric stages I and II, mean preoperative air curve >40 dB and platinotomy. The independent predictors of tinnitus regression retained were male sex, no exposure to loud noise and Veillon radiological stages I and II.

Conclusion

The identification of factors influencing the functional results of surgery allows a better selection of candidates for the intervention and more relevant information for patients.

Level of evidence

Level 3: Non-randomized controlled cohort or follow-up study.

目的评价耳硬化手术的效果，并研究其功能成功的预测因素。方法回顾性分析2000年1月至2020年12月在耳鼻喉科和头颈外科手术的322例患者，相当于418只耳朵。结果以术后残余气骨间隙(PRABG)≤10 dB为标准，89.5%的患者剪骨成功；94.3%的患者符合传导增益≥20db标准；96.4%的患者根据耳蜗储备标准进行改善；88.8%的患者符合空气骨间隙改善(ABGi)≥70%标准，81.8%的患者符合所有标准。术后短、中、长期调性听力学可叠加，反映听力结果的稳定性。据报道，79.2%的病例耳鸣消退。听力成功的独立预测因子为Aubry的听力测量I期和II期，平均术前空气曲线40 dB和铂切开术。耳鸣消退的独立预测因子为男性、无噪声暴露和威隆放射学I期和II期。结论确定影响手术功能结果的因素，可以更好地选择干预对象，为患者提供更多相关信息。证据水平：三级：非随机对照队列或随访研究。

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引用次数: 0

Expression of ApoE and Spp1 in the cochlea and auditory cortex of age-related hearing loss mice ApoE和Spp1在老年性听力损失小鼠耳蜗和听觉皮层的表达

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Brazilian Journal of Otorhinolaryngology

Pub Date : 2025-07-09 DOI: 10.1016/j.bjorl.2025.101674

Yingxue Yuan , Junhong Zhang , Jingyi Zhao , Xiru Zhang , Zhixin Cao

Objective

Screen for differential proteins in the cochlea of mice associated with Age-Related Hearing Loss (ARHL), analyze and validate the expression of specific differential proteins and genes in the cochlea and auditory cortex of ARHL mice, and preliminarily explore their potential mechanisms of action.

Methods

ABR (Auditory Brainstem Response) hearing tests were conducted to select 15-month-old C57BL/6 mice with significantly decreased hearing as the experimental group and 2-month-old mice with normal hearing as the control group. Cochleae were dissected, and unlabeled quantitative proteomics was employed to identify and analyze differentially expressed proteins in the inner ear of the two groups of mice. Key node proteins were selected via the STRING database and Cytoscape analysis. The expression of two selected proteins, ApoE and Spp1, in the cochlea was detected using qRT-PCR, Western blot, and immunofluorescence techniques, and their expression in the auditory cortex of the brain was further explored.

Results

Label-free quantitative proteomics identified 115 differentially expressed proteins in the cochlea of 15-month-old ARHL mice compared to 2-month-old hearing-normal mice, including 42 upregulated and 73 downregulated proteins. GO and KEGG enrichment analyses revealed significant enrichment of differentially expressed proteins in functions and signaling pathways associated with neurodegenerative diseases and neurotransmission. Protein-Protein Interaction (PPI) analysis using the STRING database and Cytoscape selection identified ApoE and Spp1 as key hub proteins. Validation via qRT-PCR, Western blot, and immunofluorescence demonstrated that both ApoE and Spp1 were highly expressed in the cochlea and auditory cortex of the ARHL mice compare to 2-month-old hearing-normal mice.

Conclusion

ApoE and Spp1 are upregulated in the cochlea of ARHL mice, particularly in spiral ganglion neurons, and in the auditory cortex, suggesting their potential involvement in the pathogenesis and progression of ARHL through the modulation of auditory neural conduction systems.

Level of evidence

Level 2.

目的筛选与年龄相关性听力损失（Age-Related Hearing Loss， ARHL）相关小鼠耳蜗中的差异蛋白，分析并验证ARHL小鼠耳蜗和听觉皮层中特异性差异蛋白和基因的表达，并初步探讨其可能的作用机制。方法选择15月龄听力明显下降的C57BL/6小鼠作为实验组，2月龄听力正常的小鼠作为对照组，进行听觉脑干反应（sabr）听力测试。解剖耳蜗，采用未标记定量蛋白质组学方法对两组小鼠内耳差异表达蛋白进行鉴定分析。通过STRING数据库和Cytoscape分析选择关键节点蛋白。采用qRT-PCR、Western blot和免疫荧光技术检测ApoE和Spp1两种蛋白在耳蜗中的表达，并进一步探讨其在大脑听觉皮层中的表达。结果15月龄ARHL小鼠耳蜗与2月龄听力正常小鼠相比，鉴定出115种差异表达蛋白，包括42种上调蛋白和73种下调蛋白。GO和KEGG富集分析显示，与神经退行性疾病和神经传递相关的功能和信号通路中差异表达蛋白显著富集。利用STRING数据库和Cytoscape筛选进行蛋白-蛋白相互作用（PPI）分析，确定ApoE和Spp1为关键枢纽蛋白。通过qRT-PCR、Western blot和免疫荧光验证，与2月龄听力正常的小鼠相比，ApoE和Spp1在ARHL小鼠的耳蜗和听觉皮层中均有高表达。结论apoe和Spp1在ARHL小鼠耳蜗，尤其是螺旋神经节神经元和听觉皮层中表达上调，提示它们可能通过调节听觉神经传导系统参与ARHL的发病和进展。证据等级：2级。

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引用次数: 0

Association of tinnitus with obstructive sleep apnea and rapid eye movement-related obstructive sleep apnea in similar hearing threshold groups 耳鸣与阻塞性睡眠呼吸暂停和快速眼动相关阻塞性睡眠呼吸暂停在相似听力阈组的关联

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Brazilian Journal of Otorhinolaryngology

Pub Date : 2025-07-08 DOI: 10.1016/j.bjorl.2025.101661

Sun Choi , Seok Hyun Park , Su Yeon Kim , Su Kyoung Park , Sang Chul Park , Jiwon Chang

Objectives

Obstructive sleep apnea, a multisystem disease, affects cochlear and vestibular functions due to chronic intermittent hypoxia and recurrent oxygen desaturation. During rapid eye movement sleep, the muscle tone of the upper airway decreases more, and the cardiovascular complication risk is higher than that during non-rapid eye movement sleep. We analyzed whether tinnitus was associated with obstructive sleep apnea and rapid eye movement-related obstructive sleep apnea in patients with similar hearing thresholds.

Methods

We evaluated patients with obstructive sleep apnea without (24 patients; group 1) and with (27 patients; group 2) tinnitus. All participants underwent pure-tone audiometry, tinnitus tests, polysomnography, and sleep questionnaires.

Results

The sleep efficiency (86.23% ± 10.38% vs. 81.27% ± 8.10%) and Epworth Sleepiness Scale (8.50 ± 4.31 vs. 5.15 ± 2.82) values were higher in group 2 versus group 1. In patients with rapid eye movement-related obstructive sleep apnea, the sleep efficiency value was higher in group 2 (90.55% ± 4.61%) versus group 1 (78.13% ± 11.70%). However, in patients with obstructive sleep apnea and rapid eye movement-related obstructive sleep apnea, medication use that might affect sleep was higher in group 2 (44.4% and 54.4%, respectively) versus group 1 (16.7% and 28.6%, respectively).

Conclusions

We evaluated the relationship between tinnitus and obstructive sleep apnea or rapid eye movement-related obstructive sleep apnea in groups with similar hearing thresholds for the first time. The presence of tinnitus in patients with obstructive sleep apnea with similar hearing thresholds was more closely related to subjective parameters than to objective parameters. Patients with tinnitus had poorer sleep quality than those without tinnitus.

Level of evidence

4.

目的阻塞性睡眠呼吸暂停是一种多系统疾病，慢性间歇性缺氧和反复氧饱和度降低影响耳蜗和前庭功能。快速眼动睡眠时，上气道肌张力下降幅度更大，心血管并发症风险高于非快速眼动睡眠。我们分析了耳鸣是否与听力阈值相似的患者的阻塞性睡眠呼吸暂停和快速眼动相关的阻塞性睡眠呼吸暂停有关。方法对阻塞性睡眠呼吸暂停患者(24例；1组27例；第二组耳鸣。所有参与者都进行了纯音听力学、耳鸣测试、多导睡眠描记仪和睡眠问卷调查。结果2组患者睡眠效率（86.23%±10.38% vs. 81.27%±8.10%）和Epworth嗜睡量表（8.50±4.31 vs. 5.15±2.82）均高于1组。在快速眼动相关性阻塞性睡眠呼吸暂停患者中，2组睡眠效率值（90.55%±4.61%）高于1组（78.13%±11.70%）。然而，在阻塞性睡眠呼吸暂停和快速眼动相关的阻塞性睡眠呼吸暂停患者中，2组可能影响睡眠的药物使用（分别为44.4%和54.4%）高于1组（分别为16.7%和28.6%）。结论首次在听力阈值相近的人群中评估耳鸣与阻塞性睡眠呼吸暂停或快速眼动相关性阻塞性睡眠呼吸暂停的关系。听力阈值相近的阻塞性睡眠呼吸暂停患者出现耳鸣与主观参数的关系大于与客观参数的关系。耳鸣患者的睡眠质量较无耳鸣患者差。证据水平4。

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引用次数: 0

Aberrant expression of Bcl2L12 as a potential biomarker for predicting recurrence in nasal polyp Bcl2L12的异常表达作为预测鼻息肉复发的潜在生物标志物

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Brazilian Journal of Otorhinolaryngology

Pub Date : 2025-07-04 DOI: 10.1016/j.bjorl.2025.101670

Ping Zhang , Fengjun Wang

Objective

Bcl2L12 plays an important role in the inflammatory response, but its role in chronic rhinitis with nasal polyps (CRSwNP) is unknown. We aimed to evaluate the relationship of Bcl2L12 with eosinophilic inflammation in CRSwNP and its value in predicting postoperative recurrence.

Methods

We included 80 patients with CRSwNP treated with Functional Endoscopic Sinus Surgery (FESS), and 40 Health Control (HC) subjects. The expression levels of Bcl2L12 in tissues were examined by RT-PCR, Western Blotting (WB) and Immunohistochemistry (IHC). Correlation analysis was performed to explore the relationship between the expression level of Bcl2L12 and eosinophilic inflammation of the disease. Receiver Operating Characteristics (ROC) curves were used to assess the relationship between Bcl2L12 levels and postoperative recurrence.

Results

RT-PCR results suggested that tissue Bcl2L12 levels were higher in the relapse CRSwNP group than in the control and primary CRSwNP group. Correlation analysis showed that elevated Bcl2L12 levels were positively correlated with disease eosinophilic inflammation (p < 0.05). In addition, tissue Bcl2L12 levels were higher by WB and IHC in the CRSwNP group than in the HC group (p < 0.05), especially in the recurrent group, and were centrally expressed in the epithelial region of the mucosa. Further regression analysis showed that blood eosinophil count and percentage, tissue eosinophil count, and tissue Bcl2L12 levels were closely associated with disease recurrence after surgery. The ROC curve suggested that tissue Bcl2L12 had a higher value in predicting postoperative recurrence.

Conclusion

Our study shows that Bcl2L12 is increased in patients with CRSwNP, and is associated with eosinophilic inflammation. Tissue Bcl2L12 levels may be an objective marker to predict the risk of postoperative recurrence.

Level of evidence

Level 3.

目的bcl2l12在炎症反应中发挥重要作用，但其在慢性鼻炎伴鼻息肉（CRSwNP）中的作用尚不清楚。我们的目的是评估Bcl2L12与CRSwNP中嗜酸性粒细胞炎症的关系及其在预测术后复发中的价值。方法我们纳入80例经功能性内窥镜鼻窦手术（FESS）治疗的CRSwNP患者和40例健康对照组（HC）。采用RT-PCR、Western Blotting （WB）和免疫组化（IHC）检测组织中Bcl2L12的表达水平。通过相关分析探讨Bcl2L12表达水平与疾病嗜酸性粒细胞炎症的关系。采用受试者工作特征（ROC）曲线评估Bcl2L12水平与术后复发的关系。结果rt - pcr结果显示，复发CRSwNP组组织Bcl2L12水平高于对照组和原发性CRSwNP组。相关分析显示Bcl2L12水平升高与疾病嗜酸性粒细胞炎症呈正相关（p < 0.05）。此外，CRSwNP组的组织Bcl2L12水平在WB和IHC组均高于HC组（p < 0.05），尤其是复发组，且集中表达于粘膜上皮区。进一步回归分析发现，血嗜酸性粒细胞计数和百分比、组织嗜酸性粒细胞计数和组织Bcl2L12水平与术后疾病复发密切相关。ROC曲线提示组织Bcl2L12对术后复发有较高的预测价值。结论我们的研究表明，Bcl2L12在CRSwNP患者中升高，并与嗜酸性粒细胞炎症有关。组织Bcl2L12水平可能是预测术后复发风险的客观指标。证据等级：3级。

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引用次数: 0

Bilateral middle ear cholesteatoma in children: A single-center retrospective study 儿童双侧中耳胆脂瘤：单中心回顾性研究

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Brazilian Journal of Otorhinolaryngology

Pub Date : 2025-07-03 DOI: 10.1016/j.bjorl.2025.101671

Xiaoxu Wang , Lining Guo , Enxia Tian , Min Chen , Wei Liu , Xin Ni , Jie Zhang

Objectives

To analyze the clinical characteristics of bilateral cholesteatoma in children and summarize the clinical treatment pathway.

Methods

In this retrospective cohort study, we analyzed the records of children with bilateral middle ear cholesteatoma who underwent surgery in our department between 2016 and 2023. The clinical characteristics, treatment and prognosis were analyzed.

Results

The records of 12-children (24-ears, 4girls and 8-boys) were reviewed. The mean age was 6.5 ± 2.6-years. Four children had Congenital Cholesteatoma (CC) in one ear and Acquired Cholesteatoma (AC) in the other ear and 8-children had AC in both ears. The onset time and lesion degree of the bilateral ears are different. Nine children had maxillary developmental deformities (75%). One child underwent simultaneous binaural surgery, and 11 underwent staged surgery, with a median interval of 3-months between surgeries. The follow-up period was 4.28 ± 2.58-years. No postoperative complications. Six children (7-ears) underwent revision surgery due to recurrence. There was no significant difference in hearing results between pure-tone average of preoperative (41.23 ± 16.50 dB) and postoperative (37.79 ± 14.42 dB) (p = 0.291, t = 1.082). There was no significant difference in postoperative hearing between CC and AC (p = 0.355, F = 4.268). There were significant differences in postoperation hearing among different surgical methods (p = 0.006, H = 12.630). There were significant differences in postoperation hearing among different ossiculoplasty methods (p = 0.001, H = 17.590).

Conclusions

In our cohort, the average age at diagnosis of bilateral cholesteatoma in children was notably young, with a high proportion presenting concurrent cleft palate deformities. Staged surgical intervention prioritizing the more severely affected ear was adopted for bilateral cases. Furthermore, long-term follow-up is necessary due to the observed high recurrence rate in this population. A management flowchart has been proposed based on our therapeutic experience.

Level of evidence

Level 4.

目的分析儿童双侧胆脂瘤的临床特点，总结临床治疗途径。方法回顾性队列研究，分析2016 - 2023年在我科手术治疗的双侧中耳胆脂瘤患儿。分析其临床特点、治疗及预后。结果回顾了12例24岁儿童的病历，其中女孩4例，男孩8例。平均年龄6.5±2.6岁。4例患儿单耳先天性胆脂瘤（CC），另1耳获得性胆脂瘤（AC）， 8例患儿双耳先天性胆脂瘤。双耳发病时间和病变程度不同。上颌发育畸形9例（75%）。1例患儿同时行双耳手术，11例患儿分期行手术，手术间隔中位数为3个月。随访时间为4.28±2.58年。无术后并发症。6例患儿（7耳）因复发接受翻修手术。术前纯音平均值（41.23±16.50 dB）与术后纯音平均值（37.79±14.42 dB）比较，差异无统计学意义（p = 0.291, t = 1.082）。两组术后听力差异无统计学意义（p = 0.355, F = 4.268）。不同手术方式术后听力差异有统计学意义（p = 0.006, H = 12.630）。不同听骨成形术方法术后听力差异有统计学意义（p = 0.001, H = 17.590）。结论在我们的队列中，儿童双侧胆脂瘤的平均诊断年龄明显较低，并发腭裂畸形的比例很高。双侧病例采用分阶段手术干预，优先选择受累较严重的耳部。此外，由于观察到该人群的高复发率，需要长期随访。根据我们的治疗经验，提出了一个管理流程图。证据等级：4级。

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引用次数: 0

Trends and future directions in chronic rhinosinusitis with nasal polyps: A bibliometric analysis 慢性鼻窦炎合并鼻息肉的趋势和未来方向：文献计量学分析

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Brazilian Journal of Otorhinolaryngology

Pub Date : 2025-07-03 DOI: 10.1016/j.bjorl.2025.101672

Yeming Zhong , Xuan Wei , Caiyun Zou , Bo Qian , Hongbo Ji , Jinhe Guo , Zigang Che

Objectives

Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) is a complex inflammatory condition that significantly impairs patients’ quality of life. Despite significant efforts to develop effective treatments, a comprehensive bibliometric analysis in this field is lacking. This study aims to identify key trends, influential publications, leading authors, and collaborative networks.

Methods

A bibliometric analysis was conducted on October 16, 2024, examining publications in the Web of Science Core Collection (WoSCC) database from 2004 to 2024. Data visualization and interpretation were performed using VOSviewer (version 1.6.20), CiteSpace (version 6.3.R1), and R 4.3.3.

Results

The analysis included 1,235 publications from 5,825 authors across 4,723 institutions in 222 countries. The United States led in publication volume, followed by China and Italy. Major institutional contributors included Capital Medical University and Sanofi. The International Forum of Allergy & Rhinology published the most articles, while the Journal of Allergy and Clinical Immunology had the highest citation count. Key authors identified were Bachert Claus and Gevaert Philippe. Keyword analysis revealed “asthma”, “endoscopic sinus surgery”, and “inflammation” as dominant themes, with burst analysis indicating growing interest in “humanization”, “type 2 inflammation”, “risk”, “biologics”, and “safety”.

Conclusion

This bibliometric analysis underscores an increasing focus on biologic therapies, inflammation management, and endoscopic sinus surgery in CRSwNP research. Future investigations are likely to prioritize the long-term safety and cost-effectiveness of biologics, alongside advancements in precision medicine and surgical techniques.

Level of evidence

Level 1.

慢性鼻窦炎伴鼻息肉（CRSwNP）是一种复杂的炎症性疾病，严重影响患者的生活质量。尽管为开发有效的治疗方法做出了重大努力，但在这一领域缺乏全面的文献计量分析。本研究旨在确定关键趋势、有影响力的出版物、主要作者和合作网络。方法于2024年10月16日对Web of Science Core Collection （WoSCC）数据库2004 - 2024年的出版物进行文献计量分析。使用VOSviewer（版本1.6.20）、CiteSpace（版本6.3.R1）和r4.3.3进行数据可视化和解译。该分析包括来自222个国家4723个机构的5825位作者的1235篇出版物。美国的出版物数量最多，其次是中国和意大利。主要机构捐助者包括首都医科大学和赛诺菲。国际过敏论坛；鼻科学发表的文章最多，而过敏和临床免疫学杂志的引用数最高。确定的主要作者是Bachert Claus和Gevaert Philippe。关键词分析显示，“哮喘”、“内窥镜鼻窦手术”和“炎症”是主要的主题，而突发分析显示，人们对“人性化”、“2型炎症”、“风险”、“生物制剂”和“安全性”的兴趣越来越大。该文献计量学分析强调了CRSwNP研究中对生物治疗、炎症管理和内窥镜鼻窦手术的日益关注。未来的研究可能会优先考虑生物制剂的长期安全性和成本效益，以及精准医学和外科技术的进步。证据等级：1级。

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引用次数: 0

Adequate vs. complete vs. full house surgery: What are we really talking about? 充分的、完整的、满堂红的手术：我们到底在说什么？

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Brazilian Journal of Otorhinolaryngology

Pub Date : 2025-07-01 DOI: 10.1016/j.bjorl.2025.101687

Miguel Soares Tepedino , Renato Roithmann , Fabiana Cardoso Pereira Valera , Fabrizio Ricci Romano , Eduardo Macoto Kosugi , Otávio Bejzman Piltcher , Leonardo Lopes Balsalobre Filho , Edwin Tamashiro , Wilma Terezinha Anselmo-Lima

引用次数: 0

Development and validation of prognostic prediction model for submandibular gland cancer based on the SEER database 基于SEER数据库的颌下腺癌预后预测模型的建立与验证

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Brazilian Journal of Otorhinolaryngology

Pub Date : 2025-06-25 DOI: 10.1016/j.bjorl.2025.101654

Junkun He , Feng Zhao , Jiangmiao Li, Qiyun Li, Fangyu Wei, Jiping Su

Objective

Accurately predicting the prognosis of Submandibular Gland Carcinoma (SGC) patients remains a challenging task. The purpose of this study was to develop a columnar graph prognostic prediction model for submandibular gland cancer based on the SEER database, using feature selection with lasso regression and modeling with Cox regression.

Methods

This study utilized data from the SEER database, focusing on 1362 cases of SGC. Various clinical and demographic factors, including age, tumor size, histology, and lymph node metastasis, were considered as potential prognostic factors. Feature selection was performed using lasso regression, and a Cox proportional hazards model was constructed, taking into account the complex interactions between variables and their impact on survival outcomes.

Results

The established prognostic prediction model demonstrated good accuracy and reliability. The model effectively identified several important prognostic factors, including age, tumor size, histology, and lymph node metastasis, which strongly influenced the prognosis of SGC. The model showed good discrimination and calibration with c-indexes of 0.802 (0.784‒0.821) in the training set and 0.756 (0.725‒0.787) in the validation set. The Decision Curve Analysis (DCA) curve reflected clinical utility.

Conclusion

This study suggests that the prognostic prediction model based on Cox regression is a valuable tool for predicting the prognosis of patients with SGC. This approach has the potential to improve patient outcomes by facilitating personalized treatment plans and identifying high-risk patients who may benefit from more aggressive interventions.

Level of Evidence

Level III.

目的准确预测颌下腺癌（SGC）患者的预后仍然是一项具有挑战性的任务。本研究的目的是基于SEER数据库，采用lasso回归的特征选择和Cox回归的建模，建立颌下腺癌的柱状图预后预测模型。方法利用SEER数据库资料，选取1362例SGC病例。各种临床和人口因素，包括年龄、肿瘤大小、组织学和淋巴结转移，被认为是潜在的预后因素。使用lasso回归进行特征选择，并构建Cox比例风险模型，考虑变量之间复杂的相互作用及其对生存结果的影响。结果所建立的预后预测模型具有良好的准确性和可靠性。该模型有效识别了影响SGC预后的几个重要预后因素，包括年龄、肿瘤大小、组织学和淋巴结转移。该模型在训练集和验证集上的c指数分别为0.802（0.784-0.821）和0.756(0.725-0.787)，具有良好的判别和校准能力。决策曲线分析（DCA）曲线反映临床效用。结论基于Cox回归的预后预测模型是预测SGC患者预后的一种有价值的工具。这种方法有可能通过促进个性化治疗计划和识别可能从更积极的干预中受益的高危患者来改善患者的预后。证据等级：III级。

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引用次数: 0

Cross-cultural adaptation and validation of the theory of Planned Behavior-Hearing Help Seeking: A methodological study 跨文化适应与计划行为理论的验证：一项方法论研究

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Brazilian Journal of Otorhinolaryngology

Pub Date : 2025-06-25 DOI: 10.1016/j.bjorl.2025.101660

Yuxuan He , Menghui Deng , Li Wei , Jia Liu , Xiaomei Chen , Yanni Yang

Objective

Seeking help for hearing loss is a complex psychological reaction. The purpose of this investigation was to cross-culturally adapt and validate the Theory of Planned Behavior-Hearing Help Seeking (TPB-HHS) to Chinese language and culture.

Methods

A total of 184 middle-aged and older Chinese adults completed the TPB-HHS. The study explored the factor structure and psychometric properties of the Chinese TPB-HHS, including model fit, convergent validity, discriminant validity, internal consistency and retest reliability.

Results

Four factors were identified, explaining 65% of the variance, with significant internal consistency and model fitting. In addition, they had satisfactory retest reliability after one week. In addition, the TPB-HHS scale showed substantial convergent and discriminant validity.

Conclusion

The Chinese TPB-HHS version has been shown to be a reliable and effective tool for measuring hearing test intention in initial community hearing screening. It enables healthcare providers to determine the level of intent to seek help in patients with hearing loss, laying the foundation for interventions to help these patients achieve optimal physical, psychological, and social outcomes.

Level of evidence

2.

目的听力损失患者寻求帮助是一种复杂的心理反应。本研究的目的是为了跨文化适应和验证计划行为-听力帮助寻求理论在中国语言和文化中的应用。方法对184名中国中老年成人进行TPB-HHS检查。本研究从模型拟合、收敛效度、判别效度、内部一致性和重测信度等方面探讨了中国人tbb - hhs量表的因素结构和心理测量特征。结果确定了四个因素，解释了65%的方差，具有显著的内部一致性和模型拟合。此外，他们在一周后的重测信度令人满意。此外，TPB-HHS量表具有较强的收敛效度和判别效度。结论中文TPB-HHS是一种可靠、有效的社区听力筛查中听力检测意向的测量工具。它使医疗保健提供者能够确定听力损失患者寻求帮助的意图水平，为干预措施奠定基础，帮助这些患者实现最佳的身体、心理和社会结果。证据水平2。

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引用次数: 0

CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinoma CeRNA网络揭示下咽鳞状细胞癌潜在的诊断生物标志物或免疫治疗靶点

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Brazilian Journal of Otorhinolaryngology

Pub Date : 2025-06-25 DOI: 10.1016/j.bjorl.2025.101655

Xi Yang , Chun Feng , Donghui Jiang , Xin Xu , Yingying Zhang , Jin Wang , Xiaoguang He

Objective

This study aimed to construct a ceRNA network and identify potential diagnostic biomarkers for HSCC.

Methods

The R software was used to identify Differentially Expressed circular RNAs (DE-circRNAs) and mRNAs (DE-mRNAs) between HSCC and Normal Control (NC) specimens. Univariate Cox analysis and survival curves were used to identify biomarkers associated with the prognosis of HSCC. In addition, the diagnostic value of the biomarkers and their relevance to the tumor microenvironment and immunotherapy were evaluated. A ceRNA network based on mRNAs, circRNAs, and miRNAs was established. Finally, qRT-PCR was used to evaluate the expression of biomarkers in HSCC and para-cancerous tissue samples.

Results

A network containing 90 miRNAs, 47 circRNAs, and 111 mRNAs was established via bioinformatic analysis. Three genes (NRG1, CCNG2, and CHSY1) were identified as prognostic and diagnostic biomarkers for HSCC. The low-CCNG2-expression and low-CHSY1-expression groups had higher survival rates, whereas the low-NRG1-expression group had a lower survival rate. The AUC values of the three biomarkers > 0.9, which indicated that the diagnostic value of all biomarkers was excellent. Moreover, CCNG2 and CHSY1 were associated with the severity of malignancy and the effectiveness of immunotherapy. Subsequently, a ceRNA network containing 3 mRNAs, 7 miRNAs, and 7 circRNAs was established. The results of qRT-PCR validated that the expression patterns of NRG1 and CHSY1 in clinical HSCC and para-cancerous samples were consistent with those observed in the GSE2379 dataset.

Conclusion

In this study, we identified three diagnostic biomarkers for HSCC (NRG1, CCNG2, and CHSY1) and established their corresponding ceRNA network via bioinformatic analysis, providing novel insights into the screening and treatment of HSCC.

目的构建ceRNA网络，寻找潜在的hsc诊断标志物。方法采用R软件对HSCC和正常对照（NC）标本进行差异表达环状rna （DE-circRNAs）和mrna （DE-mRNAs）的鉴定。使用单因素Cox分析和生存曲线来确定与hsc预后相关的生物标志物。此外，我们还评估了这些生物标志物的诊断价值及其与肿瘤微环境和免疫治疗的相关性。建立了基于mrna、circrna和mirna的ceRNA网络。最后，采用qRT-PCR方法评估hsc和癌旁组织样本中生物标志物的表达。结果通过生物信息学分析，构建了包含90个mirna、47个circrna和111个mrna的网络。三个基因（NRG1、CCNG2和CHSY1）被确定为hsc的预后和诊断生物标志物。ccng2低表达组和chsy1低表达组生存率较高，nrg1低表达组生存率较低。3种生物标志物的AUC值>；0.9，表明所有生物标志物的诊断价值都很好。此外，CCNG2和CHSY1与恶性肿瘤的严重程度和免疫治疗的有效性相关。随后，建立了包含3个mrna、7个mirna和7个circrna的ceRNA网络。qRT-PCR结果证实NRG1和CHSY1在临床HSCC和癌旁样本中的表达模式与GSE2379数据集中观察到的结果一致。结论本研究通过生物信息学分析，确定了三种HSCC诊断标志物NRG1、CCNG2和CHSY1，并建立了相应的ceRNA网络，为HSCC的筛查和治疗提供了新的思路。

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