Journal of Infection最新文献

{"title":"MicroRNA biomarkers and host response pathways in severe pulmonary hemorrhagic syndrome due to leptospirosis: A multi-omics study","authors":"Phu Nguyen Trong Tran ,&nbsp;Umaporn Limothai ,&nbsp;Janejira Dinhuzen ,&nbsp;Sasipha Tachaboon ,&nbsp;Theerapon Sukmark ,&nbsp;Chayomon Dokpong ,&nbsp;Sittiruk Roytrakul ,&nbsp;David A. Haake ,&nbsp;Nattachai Srisawat","doi":"10.1016/j.jinf.2024.106400","DOIUrl":"10.1016/j.jinf.2024.106400","url":null,"abstract":"<div><h3>Background</h3><div>Severe pulmonary hemorrhagic syndrome (SPHS) remains a fatal complication of leptospirosis with poorly understood mechanisms and an urgent need for effective biomarkers.</div></div><div><h3>Methods</h3><div>A nested case-control analysis was conducted using blood specimens from two previous Thai leptospirosis cohorts. Candidate microRNAs were initially discovered through a global profiling of 798 serum microRNAs in five SPHS and seven non-SPHS patients, and then validated using real-time polymerase chain reactions in 168 patients. Pathways enriched from microRNA targets were compared to those from an integrated transcriptomic-proteomic analysis. Proteins pertaining to the key resulting pathway were measured to validate significance and reveal correlation with microRNA biomarkers.</div></div><div><h3>Results</h3><div>Serum microRNA profiling revealed a total of 81 significantly expressed microRNAs, of which seven were selected for further validation in the whole cohort of 168 leptospirosis patients, including 28 in SPHS and 140 nonSPHS groups. Among the selected microRNAs, miR-5010–3p and miR-147b-3p had significantly higher expression in SPHS group compared to nonSPHS group, with consistently higher expression after adjusting for age, sex, days of illness, comorbidity, smoking status or recruitment site. The two had area under the curve (AUC) values of 0.76 (95% CI: 0.67–0.85) and 0.70 (95% CI: 0.56–0.81) for discriminating SPHS, respectively. These microRNAs also exhibited consistent AUC values in patients tested before chest radiograph shadows manifested. Combination of miR-5010–3p with miR-548ai and miR-224–5p, as selected by Bayesian Model Averaging algorithm, substantially boosts the AUC value to 0.86 (95% CI: 0.77−0.94). The miRNA biomarkers also enhanced the predictive values of a previously validated clinical model, increasing AUC value from 0.87 to 0.92 with a significant reclassification net index. Multi-omics pathway analysis incorporating microRNA targets and transcriptomic-proteomic data suggested TNF signaling as among the key pathways. In validation, seven out of ten pathway proteins were significantly different between groups, with principal components correlated with severity and miR-5010–3p.</div></div><div><h3>Conclusions</h3><div>MiR-5010-3p and miR-147b-3p are novel biomarkers with good predictability and potential relevance with TNF signaling pathway, an important host response mechanism in leptospirosis SPHS.</div></div>","PeriodicalId":50180,"journal":{"name":"Journal of Infection","volume":"90 2","pages":"Article 106400"},"PeriodicalIF":14.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Individual patient and donor seroprofiles in convalescent plasma treatment of COVID-19 in REMAP-CAP clinical trial","authors":"Visa Nurmi ,&nbsp;Richard Mayne ,&nbsp;Chanice Knight ,&nbsp;Hannia L. Almonacid-Mendoza ,&nbsp;Shannah Secret ,&nbsp;Lise Estcourt ,&nbsp;Jussi Hepojoki ,&nbsp;Tonći Šuštić ,&nbsp;Abigail A. Lamikanra ,&nbsp;Hoi Pat Tsang ,&nbsp;David K. Menon ,&nbsp;Manu Shankar-Hari ,&nbsp;C. Ellen van der Schoot ,&nbsp;Gestur Vidarsson ,&nbsp;David J. Roberts ,&nbsp;Peter Simmonds ,&nbsp;Klaus Hedman ,&nbsp;Heli Harvala","doi":"10.1016/j.jinf.2025.106412","DOIUrl":"10.1016/j.jinf.2025.106412","url":null,"abstract":"<div><h3>Objectives</h3><div>Convalescent plasma (CP) treatment of COVID-19 has shown significant therapeutic effect only when administered early. We investigated the importance of patient and CP seroprofiles on treatment outcome in REMAP-CAP CP trial.</div></div><div><h3>Methods</h3><div>We evaluated neutralising antibodies (nAb), anti-spike (S) IgM, IgG, IgG avidity, IgG fucosylation and respiratory viral loads in a sub-set of patients (n=80) and controls (n=51) before and after transfusion, comparing them to those in the CP units (n=157) they received.</div></div><div><h3>Results</h3><div>Most patients were SARS-CoV-2 seropositive pre-transfusion (72% nAb; 89% S-IgG seropositivity). The majority (80%) had higher pre-transfusion S-IgG levels (median 1.7×10⁶ arbitrary units (AU); 56%) or S-IgG production rates (median 1.1×10⁶ AU/day; 64%) than they received from CP (median 2.2×10⁵ AU). Only 22% of the patients demonstrated significant (median 24-fold) increase in their S-IgG levels acquired from transfusion. Better outcomes, measured by organ support-free days, were associated with increase in S-IgM levels (p=0.007), decreased S-IgG fucosylation (p&lt;0.001), lower patient age (p&lt;0.001) but not with receiving CP (p=0.337).</div></div><div><h3>Conclusions</h3><div>Based on our data, increased S-antibody levels linked to transfused CP were only observed in pre-seroconversion or immunodeficient patients lacking their own SARS-CoV-2 antibodies, representing the groups where CP treatment has previously shown most benefit.</div></div>","PeriodicalId":50180,"journal":{"name":"Journal of Infection","volume":"90 2","pages":"Article 106412"},"PeriodicalIF":14.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142973069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic performance of metagenomic next-generation sequencing among hematological malignancy patients with bloodstream infections after antimicrobial therapy","authors":"Yueyi Xu ,&nbsp;Miaoxin Peng ,&nbsp;Tong Zhou ,&nbsp;Yonggong Yang ,&nbsp;Peipei Xu ,&nbsp;Ting Xie ,&nbsp;Xuefang Cao ,&nbsp;Bing Chen ,&nbsp;Jian Ouyang","doi":"10.1016/j.jinf.2024.106395","DOIUrl":"10.1016/j.jinf.2024.106395","url":null,"abstract":"<div><h3>Background</h3><div>Metagenomic next-generation sequencing (mNGS) is an effective method for detecting pathogenic pathogens of bloodstream infection (BSI). However, there is no consensus on whether the use of antibiotics affects the diagnostic performance of mNGS. We conducted a prospective clinical study aiming to evaluate the effect of antimicrobial treatment on mNGS.</div></div><div><h3>Methods</h3><div>Blood samples were collected for mNGS testing within 24 h of culture-confirmed with BSI, with re-examination conducted every 2–3 days.</div></div><div><h3>Results</h3><div>A total of 38 patients with BSI were enrolled. The mNGS positive (mNGS-pos) rate declined sharply after the use of antibiotics, with only 17 (44.78%) patients remaining mNGS-pos while the rest were mNGS negative (mNGS-neg). The median duration of pathogen identification was significantly longer for mNGS compared to blood culture (BC) (4 days vs 1 days; P &lt; 0.0001). A positivity duration of ≥ 3 days was an independent risk factor of septic shock (OR, 20.671; 95% CI, 1.958–218.190; P = 0.012). Patients with mNGS-pos and mNGS-neg differed by the median duration of fever (6 days vs 3 days; P = 0.038), rates of drug resistance (35.3% vs 4.8%; P = 0.017), rates of septic shock (47.1% vs 14.3%; P = 0.029), and 28-day mortality (29.4% vs 4.8%; P = 0.041).</div></div><div><h3>Conclusions</h3><div>The antimicrobial treatment will greatly reduce the positive rate of mNGS. The duration of mNGS is significantly longer than that of BC. The prolonged duration of mNGS suggests an increased risk of septic shock and could be identified as a high-risk factor of adverse infection outcome, requiring more aggressive anti-infective treatment measures.</div></div>","PeriodicalId":50180,"journal":{"name":"Journal of Infection","volume":"90 2","pages":"Article 106395"},"PeriodicalIF":14.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of interethnic lipidomic variation in falciparum malaria","authors":"Wael Abdrabou ,&nbsp;Saruul Zorigt ,&nbsp;Issiaka Soulama ,&nbsp;Dariga Bolatbay ,&nbsp;Mame Massar Dieng ,&nbsp;Jakub Jurkovic ,&nbsp;Samuel Sindié Sermé ,&nbsp;Salif Sombié ,&nbsp;Noëlie Béré Henry ,&nbsp;Désiré Kargougou ,&nbsp;Sam Aboubacar Coulibaly ,&nbsp;Aïssatou Diawara ,&nbsp;Youssef Idaghdour","doi":"10.1016/j.jinf.2024.106396","DOIUrl":"10.1016/j.jinf.2024.106396","url":null,"abstract":"<div><h3>Background</h3><div>Shifts in dietary patterns during lifestyle transitions are integral components of the dynamic interactions between humans and their environments. Investigating the link between dietary diversity, the composition of the human lipidome and infection is key to understanding the interplay between diet and susceptibility to pathogens.</div></div><div><h3>Methods</h3><div>Here we address this question by performing a comparative study of two ethnic groups with divergent dietary patterns: Fulani, who are nomad pastoralists with a dairy-centric diet, and Mossi, who are farmers with a plant-based diet. We generate 196 paired global lipidomes (927 lipid molecules) from both groups before and during natural <em>Plasmodium falciparum</em> infection.</div></div><div><h3>Results</h3><div>Our analysis revealed 211 significantly differentially abundant lipid molecules between the two ethnic groups in both infection states. We show that ethnicity has a greater impact on the lipidome of these children than do <em>P. falciparum</em> infection and report inter-ethnic differences that impact pathogenesis. We highlight elevated levels of pentadecanoic acid (C15:0)-containing phospholipids in Fulani and experimentally demonstrate the suppressive effects of lysophosphatidylcholine LysoPC (15:0) on <em>P. falciparum</em> gametocyte production.</div></div><div><h3>Conclusion</h3><div>These findings link the Fulani’s dairy-centric diet and lower <em>P. falciparum</em> gametocyte densities reported in this group and underscore the intricate links between dietary lipids and the host response to infection.</div></div>","PeriodicalId":50180,"journal":{"name":"Journal of Infection","volume":"90 2","pages":"Article 106396"},"PeriodicalIF":14.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Initial antigen encounter determines robust T-cell immunity against SARS-CoV-2 BA.2.86 variant three years later","authors":"Rocío Rubio ,&nbsp;Alexei Yavlinsky ,&nbsp;Marina Escalera Zamudio ,&nbsp;Luis M. Molinos-Albert ,&nbsp;Carla Martín Pérez ,&nbsp;Edwards Pradenas ,&nbsp;Mar Canyelles ,&nbsp;Cèlia Torres ,&nbsp;Cedric Tan ,&nbsp;Leo Swadling ,&nbsp;Anna Ramírez-Morros ,&nbsp;Benjamin Trinité ,&nbsp;Josep Vidal-Alaball ,&nbsp;Ruth Aguilar ,&nbsp;Anna Ruiz-Comellas ,&nbsp;Julià Blanco ,&nbsp;Lucy van Dorp ,&nbsp;François Balloux ,&nbsp;Carlota Dobaño ,&nbsp;Gemma Moncunill","doi":"10.1016/j.jinf.2024.106402","DOIUrl":"10.1016/j.jinf.2024.106402","url":null,"abstract":"<div><h3>Objectives</h3><div>We aimed to evaluate the adaptive immune responses cross-recognition of the hypermutated SARS-CoV-2 BA.2.86 variant and identify the determinants influencing this recognition.</div></div><div><h3>Methods</h3><div>We measured BA.2.86 neutralizing antibodies and T-cell responses cross-reactivity in previously exposed participants. We investigated clinical-demographic factors and used a novel in silico analysis to assess viral genetic determinants affecting T-cell responses.</div></div><div><h3>Results</h3><div>Despite notable escape from neutralizing antibodies, T-cell responses remained generally preserved, albeit with a significant but small loss in T-cell cross-recognition (7.5%, 14.2%, and 10.8% average loss for IFN-γ, IL-2, and IFN-γ + IL-2, respectively, p&lt;0.05). This is consistent with the prediction of 6 out of 10 immunodominant T-cell epitopes (TCEs) altered by BA.2.86 mutations to have reduced peptide presentation. This effect is expected to be mitigated by total TCEs across the genome. Remarkably, T-cell responses and cross-recognition were 3.5 (IFN-γ), 2 (IL-2) and 2.4 (IFN-γ + IL-2) times higher when first induced by infection rather than by vaccination three years earlier, by increasing number of infections, and by ancestral/Delta than Omicron infections.</div></div><div><h3>Conclusions</h3><div>Our findings underscore the critical role and factors influencing T-cell immunity against evolving SARS-CoV-2 variants, such as first antigen encounter (vaccination or infection), as it is essential for developing effective control strategies.</div></div>","PeriodicalId":50180,"journal":{"name":"Journal of Infection","volume":"90 2","pages":"Article 106402"},"PeriodicalIF":14.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Declining COVID-19 vaccination coverage in Brazil: A global health warning","authors":"Milena Silva Souza,&nbsp;Jéssica Pires Farias,&nbsp;Luís Carlos de Souza Ferreira,&nbsp;Jaime Henrique Amorim","doi":"10.1016/j.jinf.2025.106418","DOIUrl":"10.1016/j.jinf.2025.106418","url":null,"abstract":"","PeriodicalId":50180,"journal":{"name":"Journal of Infection","volume":"90 2","pages":"Article 106418"},"PeriodicalIF":14.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-copy IncP-2 megaplasmid carrying blaAFM in clinical difficult-to-treat resistance Pseudomonas aeruginosa: Associated with high-level cefiderocol resistance","authors":"Wenhao Wu ,&nbsp;Meijun Song ,&nbsp;Xi Li,&nbsp;Piaopiao Zhang,&nbsp;Yue Li,&nbsp;Heng Cai,&nbsp;Yan Jiang,&nbsp;Yunsong Yu,&nbsp;Tingting Qu","doi":"10.1016/j.jinf.2025.106422","DOIUrl":"10.1016/j.jinf.2025.106422","url":null,"abstract":"","PeriodicalId":50180,"journal":{"name":"Journal of Infection","volume":"90 2","pages":"Article 106422"},"PeriodicalIF":14.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The need for inclusion of the Hib vaccine in mainland national immunization program in China","authors":"Yonghong Yang,&nbsp;Xuzhuang Shen,&nbsp;Huili Hu,&nbsp;Zhiqin Leng,&nbsp;Yajuan Wang,&nbsp;Dingle Yu,&nbsp;Jun Yin","doi":"10.1016/j.jinf.2024.106386","DOIUrl":"10.1016/j.jinf.2024.106386","url":null,"abstract":"","PeriodicalId":50180,"journal":{"name":"Journal of Infection","volume":"90 2","pages":"Article 106386"},"PeriodicalIF":14.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shikimic acid enhances vancomycin efficacy in experimental MRSA-induced infective endocarditis","authors":"Ting-Yin Lu,&nbsp;Long-Gen Zhong,&nbsp;Jun-Qi Liu,&nbsp;Chuan-Jian Zhang,&nbsp;Zuo-Zhong Zhu,&nbsp;Meng-Ting Tao,&nbsp;Xiao-Ping Liao,&nbsp;Jian Sun,&nbsp;Yu-Feng Zhou","doi":"10.1016/j.jinf.2024.106389","DOIUrl":"10.1016/j.jinf.2024.106389","url":null,"abstract":"","PeriodicalId":50180,"journal":{"name":"Journal of Infection","volume":"90 2","pages":"Article 106389"},"PeriodicalIF":14.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The global prevalence of reported penicillin allergy: A systematic review and meta-analysis","authors":"Akish Luintel ,&nbsp;Joseph Healy ,&nbsp;Michael Blank ,&nbsp;Aashika Luintel ,&nbsp;Simon Dryden ,&nbsp;Abhishek Das ,&nbsp;Ara Darzi ,&nbsp;Graham Cooke","doi":"10.1016/j.jinf.2025.106429","DOIUrl":"10.1016/j.jinf.2025.106429","url":null,"abstract":"<div><h3>Objectives</h3><div>Patients labelled with penicillin allergy (PenA) often receive broader spectrum antibiotics, associated with antimicrobial resistance and poorer outcomes. However, ∼95% of patients are likely mis-labelled. Whilst de-labelling programmes are gaining momentum, they have been restricted to a few countries. Here, we address the global prevalence of PenA, to inform the wider potential impact for de-labelling programmes.</div></div><div><h3>Methods</h3><div>We conducted a systematic review and meta-analysis including all studies on adult PenA prevalence between January 2003 and June 2023. Data on PenA prevalence, allergy recording methods, healthcare setting, and country income were extracted. This study is registered on PROSPERO (CRD42023437718).</div></div><div><h3>Results</h3><div>174 studies from 28 countries were included (18,352 screened). Global PenA prevalence was 9·4% (95% CI 8·4–10·4%). 92% of peer-reviewed publications were from high-income countries(HICs), with 72% from the UK, USA or Australia. HICs had higher PenA prevalence 9·9% (95% CI 8·8–11·0%), compared to middle-income countries (MICs), 4·4% (95% CI 2·8–6·2%), p&lt;0.0001. Primary care data was seldom reported (16% of studies), and the method of allergy recording significantly influenced reported prevalence.</div></div><div><h3>Conclusions</h3><div>Studies reporting PenA prevalence are skewed towards HICs and secondary care, with little data from Africa, most of Asia and South America. This highlights an unmet need to broaden epidemiological analysis in under-represented regions.</div></div>","PeriodicalId":50180,"journal":{"name":"Journal of Infection","volume":"90 2","pages":"Article 106429"},"PeriodicalIF":14.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143061299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}