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Obsidian sourcing in Mesoamerica and the Isthmo-Colombian area using image-based machine learning 使用基于图像的机器学习在中美洲和哥伦比亚地峡地区采购黑曜石
IF 2.5 1区 地球科学 Q1 ANTHROPOLOGY Pub Date : 2026-01-17 DOI: 10.1016/j.jas.2026.106486
Mike Lyons , Jeannine Langmann , Markus Reindel
This study explores the use of convolutional neural networks (CNNs) to classify archaeological obsidian artifacts by their geological source using standard documentation photographs. Drawing on a dataset of images taken under varied field and laboratory conditions, we train and evaluate multiple CNN architectures to assess the feasibility of this approach as a low-cost alternative to geochemical sourcing. The models achieve high precision and recall for several well-represented sources, and Grad-CAM visualizations indicate that classification is often based on visually meaningful surface features. This work represents a first step towards developing a new sourcing methodology, as the technique demonstrates strong potential for scaling up obsidian sourcing in contexts where access to laboratory equipment is limited or cost-prohibitive. We argue that with a larger and more diverse image dataset—including a broader range of artifact types and source locations—image-based classification could become a practical and accessible tool for archaeological research in Middle America and beyond.
本研究探索了使用卷积神经网络(cnn)根据其地质来源使用标准文档照片对考古黑曜石文物进行分类。利用在不同现场和实验室条件下拍摄的图像数据集,我们训练和评估了多个CNN架构,以评估这种方法作为地球化学来源的低成本替代方案的可行性。该模型对几个代表性良好的数据源实现了高精度和召回率,并且Grad-CAM可视化表明分类通常基于视觉上有意义的表面特征。这项工作代表了开发一种新的采购方法的第一步,因为该技术显示了在获得实验室设备有限或成本过高的情况下扩大黑曜石采购的强大潜力。我们认为,有了更大、更多样化的图像数据集——包括更广泛的人工制品类型和来源位置——基于图像的分类可能成为中美洲及其他地区考古研究的实用和可访问的工具。
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引用次数: 0
Spatial and temporal variation in the diversity, abundance, and energetic returns of underground storage organs on the coastal lowlands of the Cape Floristic Region, South Africa 南非开普植物区沿海低地地下贮藏器官多样性、丰度和能量回报的时空变化
IF 2.5 1区 地球科学 Q1 ANTHROPOLOGY Pub Date : 2026-01-17 DOI: 10.1016/j.jas.2026.106471
Elzanne Singels , Tineke Kraaij , John Parkington , Karen Esler , Richard M. Cowling
A rich, abundant, and nutritious flora with underground storage organs (USOs) is deemed an important energy source for Stone Age hunter-gatherers in the Cape Floristic Region (CFR) of South Africa. Research on USO richness, abundance, and energetic returns is largely confined to the south coast of the CFR. We extend this research to the west and southeast coasts by surveying 212 sites equally in the growing (spring) and dormant (autumn) seasons and across three biomes in all three regions. We quantify USO apparency (visibility above ground) to foragers based on phenological patterns of 131 edible USO species across CFR coastal lowlands. Apparency is seasonally constrained to cooler months in the strongly winter-rainfall west coast but less seasonal along the bimodal-rainfall south coast, and non-seasonal-rainfall east coast, providing longer periods for foraging. In all the surveyed regions, richness, abundance, and energetic returns of edible USOs peaked in winter/spring and were dominated by high-energy cormous species. On an annual basis, energetic returns were highest on the south coast, owing to USO apparency peaking in autumn, a feature absent at the other sites. In all regions, hotspots of USO energetic returns were rare, but most common on the south coast. Energetic returns were mostly higher in Strandveld than in Fynbos and Renosterveld. We discuss the implications of these findings for forager mobility in the three regions of the Cape coastal lowlands.
在南非开普植物区(CFR),一种具有地下储存器官(USOs)的丰富、丰富和营养丰富的植物群被认为是石器时代狩猎采集者的重要能源来源。关于USO丰富度、丰度和能量回报的研究主要局限于CFR的南海岸。我们将这项研究扩展到西部和东南海岸,在生长(春季)和休眠(秋季)季节调查了212个地点,跨越了三个地区的三个生物群系。根据CFR沿海低地131种可食用USO物种的物候模式,我们量化了采集者的USO可见度(地面上的可见度)。显然,在冬季强降雨的西海岸,季节性限制在较凉爽的月份,而在双峰降雨的南海岸和非季节性降雨的东海岸,季节性较少,为觅食提供了更长的时间。在所有调查区域,可食USOs的丰富度、丰度和能量回报均在冬/春季达到峰值,且以高能状荚类为主。在每年的基础上,由于USO明显在秋季达到峰值,南海岸的活力回报最高,这是其他地区所没有的特征。在所有地区,USO积极回归的热点都很少见,但在南海岸最为常见。Strandveld的活力回报大多高于Fynbos和Renosterveld。我们讨论了这些发现对开普沿海低地三个地区的觅食者流动性的影响。
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引用次数: 0
Deep learning-based morphological classification of ceramics: A case study of 3D point cloud analysis for Sue ware, Japan 基于深度学习的陶瓷形态分类:以日本苏瓷三维点云分析为例
IF 2.5 1区 地球科学 Q1 ANTHROPOLOGY Pub Date : 2026-01-16 DOI: 10.1016/j.jas.2026.106472
Wataru Tatsuda , Ryo Hori , Kimiyasu Morikawa , Hayata Inoue
This study presents an innovative deep learning-based method for the morphological classification of 3D point cloud data of ceramics. It focuses on a case study of ancient pottery known as Sue ware from the Sanage kiln in Japan from the 8th to the mid-9th century. Our workflow — with a cutting-edge deep learning model, PointTransformer — outperforms previous 2D outline-based approaches, requiring only a lightweight point cloud dataset and reasonable hardware. Our model successfully classifies 917 vessel samples into labeled classes with high accuracy (average macro F1-score: 0.9320). Principal Component Analysis, hierarchical clustering, and 3D Grad-CAM saliency maps revealed the geometric cues underlying the network’s decisions, transforming it from a “black box” into an interpretable analytical tool. Additionally, we have open-sourced our dataset and code to support other researchers in customizing the model for broader archaeological applications. The study demonstrates that deep learning models can capture subtle morphological variation at scale, offering archaeologists a flexible, transparent, and reproducible method for ceramic classification that can be extended to other regions, periods, and research questions.
提出了一种基于深度学习的陶瓷三维点云数据形态分类方法。它重点研究了8世纪至9世纪中期日本Sanage窑的古代陶器Sue ware的案例。我们的工作流程-使用尖端的深度学习模型PointTransformer -优于以前基于2D大纲的方法,只需要轻量级的点云数据集和合理的硬件。我们的模型成功地将917个血管样本分类为标记类,准确率很高(平均宏观F1-score: 0.9320)。主成分分析、分层聚类和3D Grad-CAM显著性图揭示了网络决策背后的几何线索,将其从“黑盒子”转变为可解释的分析工具。此外,我们已经开放了我们的数据集和代码,以支持其他研究人员为更广泛的考古应用定制模型。该研究表明,深度学习模型可以在规模上捕捉细微的形态变化,为考古学家提供了一种灵活、透明和可重复的陶瓷分类方法,可以扩展到其他地区、时期和研究问题。
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引用次数: 0
Distinguishing biogenic from geogenic anhydrites in ash: a case study of Iron Age Qumran 灰中生物成因硬石膏与地成因硬石膏的区分——以铁器时代库姆兰为例
IF 2.5 1区 地球科学 Q1 ANTHROPOLOGY Pub Date : 2026-01-15 DOI: 10.1016/j.jas.2026.106477
Ilana Peters , Elisabetta Boaretto , Mark Cavanagh , Amos Frumkin , Ofer Sion , Yotam Asscher
A white layer composed of anhydrite, a calcium sulfate mineral, was identified in an archaeological context within a cave near the Dead Sea, radiocarbon dated to the Iron Age period. Under ambient conditions, anhydrite typically hydrates to gypsum, rendering its retention a notably rare phenomenon in an archaeological setting. This prompted questions regarding both its preservation and origin. Certain plants, such as tamarisk, are known to produce calcium sulfate hemihydrate, which dehydrates to anhydrite when burned. Similarly, geogenic gypsum converts to anhydrite upon heating, as occurs in gypsum plaster production. Based on this, we hypothesized that the archaeological layer formed as a result of thermal processes. In this study, we demonstrate that it is possible to distinguish between pyrogenic anhydrite derived from biological versus geological sources. To do so, we conducted controlled heating experiments on geological and synthetic calcium sulfates, as well as on various tamarisk tissues, including bark, green branches, and woody parts. These experiments enabled us to quantify changes in chemical composition, morphology, and atomic-scale structural disorder caused by heating. The structural disorder was assessed using a novel infrared spectroscopic approach we developed, which tracks changes in anhydrite vibrational bands. Our results indicate that the thick anhydrite layer in Qumran Cave 49 originated from the burning of green tamarisk branches circa 2800 years ago, providing new constraints on hydration processes in hyper-arid conditions. This conclusion is supported by evidence of disordered crystallinity, elevated sodium content, and irregular particle morphology of the crystals. The methodology presented here offers a new avenue for identifying the origin of calcium sulfate materials in archaeological contexts, including gypsum plaster found at sites distant from geological gypsum sources. It can help determine whether local biological sources were exploited or if geological materials were acquired through trade.
在死海附近的一个洞穴的考古背景中,发现了一层由硬石膏(一种硫酸钙矿物)组成的白色层,放射性碳可以追溯到铁器时代。在环境条件下,硬石膏通常会水化成石膏,这使得它在考古环境中保持不变是一种非常罕见的现象。这引发了关于它的保存和起源的问题。某些植物,如红柳,会产生半水合硫酸钙,燃烧后会脱水成硬石膏。同样地,地质石膏在加热时转化为硬石膏,就像石膏生产中发生的那样。基于此,我们假设考古层的形成是热过程的结果。在这项研究中,我们证明了区分生物源和地质源的热原硬石膏是可能的。为此,我们对地质硫酸钙和合成硫酸钙以及红柳的各种组织(包括树皮、绿枝和木质部分)进行了对照加热实验。这些实验使我们能够量化由加热引起的化学成分、形态和原子尺度结构紊乱的变化。使用我们开发的一种新的红外光谱方法来评估结构紊乱,该方法可以跟踪硬石膏振动带的变化。我们的研究结果表明,昆兰49洞的厚硬石膏层起源于2800年前绿柽柳枝的燃烧,为超干旱条件下的水化过程提供了新的约束条件。这一结论得到了晶体无序结晶度、钠含量升高和不规则颗粒形态的证据的支持。本文提出的方法为在考古背景下识别硫酸钙材料的来源提供了一种新的途径,包括在远离地质石膏来源的地点发现的石膏。它可以帮助确定是否利用了当地的生物资源,或者是否通过贸易获得了地质材料。
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引用次数: 0
Land management during the Bronze Age in the Upper Seine Valley (France): Isotopic evidence for selective land-use 上塞纳河谷(法国)青铜时代的土地管理:选择性土地利用的同位素证据
IF 2.5 1区 地球科学 Q1 ANTHROPOLOGY Pub Date : 2026-01-13 DOI: 10.1016/j.jas.2026.106474
Solène Chevallier , Hannah F. James , Rebecca Peake , Christophe Snoeck , Estelle Herrscher
During the Bronze Age, the Upper Seine Valley, in northeastern France, was a dynamic region, at the confluence of two rivers and at the crossroads of two cultural entities. Initially aligned with the Atlantic cultural sphere, the area experienced an increasing influence from the Tumulus culture between the 14th and 11th centuries BCE. This interaction led to significant transformations in material culture and funerary practices, notably with cremations becoming the main rite. To understand the underlying factors behind these changes, the study focuses on human mobility and interactions during this period. Strontium isotope (87Sr/86Sr) analyses were conducted on 100 cremated human bones from eight archaeological sites in the Upper Seine Valley. In parallel, 96 modern plants were analysed to develop a high-density local bioavailable strontium isoscape. The results reveal the presence of two isotopically distinct human groups, each associated with different land-use strategies. While part of the population sourced their food from the southern Yonne river basin, others favoured the Seine-Yonne confluence area, suggesting structured patterns of resource acquisition. In addition, isotopic outliers indicate mobility between these groups, with one case likely reflecting maternal mobility during pregnancy. With a detailed local baseline and a method adapted to cremated remains, this study provides a refined understanding of population dynamics at the end of the Bronze Age. Working within a well-documented archaeological context and focusing on a restricted area enabled a new understanding of land-use dynamics in the Upper Seine Valley during a key period of cultural transition.
在青铜时代,法国东北部的上塞纳河谷是一个充满活力的地区,位于两条河流的交汇处,也是两种文化实体的十字路口。最初与大西洋文化圈结盟,该地区在公元前14世纪至11世纪期间受到Tumulus文化的影响越来越大。这种互动导致了物质文化和丧葬习俗的重大转变,特别是火葬成为主要仪式。为了了解这些变化背后的潜在因素,本研究将重点放在这一时期人类的流动性和相互作用上。对来自上塞纳河流域8个考古遗址的100具火化人骨进行了锶同位素(87Sr/86Sr)分析。同时,对96种现代植物进行了分析,以建立高密度的本地生物可利用锶等景。结果显示存在两个同位素不同的人类群体,每个群体都与不同的土地利用策略相关。虽然部分人口从南部约纳河流域获取食物,但其他人更喜欢塞纳-约纳汇合处,这表明资源获取的结构性模式。此外,同位素异常值表明这些群体之间的流动性,其中一个案例可能反映了孕妇在怀孕期间的流动性。通过详细的当地基线和适用于火化遗骸的方法,本研究提供了对青铜器时代末期人口动态的精细理解。在一个有充分文献记载的考古背景下工作,并专注于一个有限的区域,使人们对塞纳河上游流域在文化转型的关键时期的土地利用动态有了新的认识。
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引用次数: 0
Bioarchaeological signatures during the Plague of Justinian (541–750 CE) in Jerash (ancient Gerasa), Jordan 约旦杰拉什(古格拉萨)查士丁尼瘟疫时期(541-750年)的生物考古特征
IF 2.5 1区 地球科学 Q1 ANTHROPOLOGY Pub Date : 2026-01-13 DOI: 10.1016/j.jas.2026.106473
Karen Hendrix , Swamy R. Adapa , Robert H. Tykot , Gregory O'Corry-Crowe , Andrea Vianello , Gloria C. Ferreira , Michael Decker , Rays H.Y. Jiang
Jerash (ancient Gerasa, in modern day Jordan) reached its demographic peak in the 3rd century CE with a population of roughly 25,000, but by the end of the 6th century this had declined to about 10,000, setting the stage for the urban vulnerabilities examined in this study. The W2 and W3 chambers of the Jerash hippodrome contain a densely layered mass burial of ∼230 individuals dating to the mid-6th to early 7th century AD. Through archaeological documentation, stable isotope analysis, and ancient DNA study, we present the first biomolecularly confirmed mass grave associated with the First Pandemic (Justinianic Plague) in the Eastern Mediterranean. The taphonomic pattern, rapid, high-density deposition with minimal funerary structuring, closely parallels catastrophic plague pits of the later medieval period, making Jerash a uniquely well-preserved example from Late Antiquity.
Stable carbon and nitrogen isotope values from human bone collagen indicate diets dominated by C3 resources typical of the region. In contrast, oxygen isotope values from tooth enamel display a markedly wider range than those documented in long-term residential populations at Tell Dothan, Pella, or Faynan in the Levant. Although oxygen isotopes cannot specify geographic origin, the magnitude of variation, arising within a burial event deposited over only days or weeks, suggests that the individuals interred in the Jerash mass grave grew up in diverse childhood water ecologies. We interpret this pattern conservatively as evidence of heterogeneous lived experiences among the victims during the crisis.
Ancient DNA analysis has recently identified a single, uniform strain of Yersinia pestis, confirming a synchronous epidemic event. In this study, mitochondrial haplogroups H13 and L3e were detected among the victims and fall within the expected maternal diversity of the Byzantine Levant.
Taken together, the archaeological, isotopic, and genetic results establish Jerash as the earliest securely identified catastrophic plague burial in the Near East. The First Pandemic concentrated a potentially socially and geographically heterogeneous population into a single mortuary event, providing a rare empirical window into mobility, urban life, and vulnerability in Late Antiquity. Jerash thus offers a critical anchor point for reconstructing the demographic and epidemiological landscape of the early medieval Mediterranean.
杰拉什(古格拉萨,今约旦)在公元3世纪达到人口高峰,人口约为25,000人,但到6世纪末,人口数量下降到约10,000人,为本研究中研究的城市脆弱性奠定了基础。杰拉什竞技场的W2和W3墓室包含一个密集的层状集体墓葬,大约有230人,可以追溯到公元6世纪中期到7世纪初。通过考古文献、稳定同位素分析和古代DNA研究,我们提出了与东地中海第一次大流行(查士丁尼大瘟疫)有关的第一个生物分子证实的万人坑。埋藏模式,快速、高密度的沉积和最小的丧葬结构,与中世纪后期的灾难性瘟疫坑非常相似,使杰拉什成为古代晚期保存完好的独特例子。
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引用次数: 0
Multidisciplinary analysis reveals genetic heterogeneity and millet farmer ancestry in late Xianbei individuals 多学科分析揭示了鲜卑晚期个体的遗传异质性和谷子农民祖先
IF 2.5 1区 地球科学 Q1 ANTHROPOLOGY Pub Date : 2026-01-10 DOI: 10.1016/j.jas.2026.106479
Danyang Ge , Chen Duan , Zhizhong Zhang , Hailun Zhang , Jiashuo Zhang , Jiaqi Jin , Ruiqi Zou , Quanchao Zhang , Xiaoting Jing , Jianing He , Chao Ning
The Xianbei were a prominent nomadic group in Chinese history, whose origins, cultural practices and subsistence strategies have long attracted scholarly attention. Despite previous studies, the genetic composition, migration history and integration of the Tuoba Xianbei into northern China remain poorly understood. To address these, we generated 39 ancient genomes, 66 stable carbon and nitrogen isotope measurements and 8 AMS radiocarbon dates from the Shanghuaju (SHJ) archaeological site in Datong, Shanxi, dating to the Pingcheng period of the Northern Wei dynasty. Our analyses reveal a complex genetic structure within the SHJ population, reflecting extensive admixture among local Han agriculturalists, ancient northeast Asian groups, Western Steppe herders and Near Eastern populations. While most individuals show ancestry related to Yellow River Basin populations, a subset retains genetic affinities with early Xianbei populations from the Greater Khingan Mountains, providing evidence for partial continuity with their ancestral lineage and supporting historical accounts of the Tuoba Xianbei's Donghu origin. Several individuals exhibit detectable Near Eastern ancestry, consistent with long-distance gene flow along the Eurasian Steppe during the Northern Wei dynasty. Stable isotope analyses indicate a predominantly C4-based diet supplemented with moderate animal protein, with males and females showing minor differences in intake, reflecting potential sex-biased dietary patterns. These findings highlight the high genetic diversity of the SHJ population and illustrate how migration, admixture and cultural integration shaped the demographic, dietary and cultural landscape of the Tuoba Xianbei in northern China.
鲜卑人是中国历史上一个重要的游牧民族,其起源、文化习俗和生存策略一直备受学术界关注。尽管已有研究,但拓跋族鲜卑人的遗传组成、迁徙历史和融入中国北方的情况仍知之甚少。为了解决这些问题,我们在山西大同上花居(SHJ)考古遗址中生成了39个古代基因组,66个稳定的碳和氮同位素测量结果和8个AMS放射性碳测年,这些测年可追溯到北魏平城时期。我们的分析揭示了SHJ人群中复杂的遗传结构,反映了当地汉族农人、古代东北亚群体、西部草原牧民和近东人群之间的广泛混合。虽然大多数人的祖先与黄河流域人群有关,但有一部分人与大兴安山区早期鲜卑人群保持遗传亲缘关系,这为其祖先谱系的部分连续性提供了证据,并支持了拓跋鲜卑人东湖起源的历史记载。几个个体表现出可检测的近东血统,与北魏时期沿欧亚草原的长距离基因流动相一致。稳定同位素分析表明,主要以c4为基础的饮食补充了适量的动物蛋白,雄性和雌性的摄入量差异不大,反映了潜在的性别偏见饮食模式。这些发现突出了SHJ人群的高度遗传多样性,并说明了迁移、混合和文化融合如何塑造了中国北方拓跋鲜卑的人口、饮食和文化景观。
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引用次数: 0
Enhancing quarry landscape interpretation with UAV LiDAR and morphometric clustering: A case study from the Euganean Hills, Italy 利用无人机激光雷达和形态测量聚类增强采石场景观解释:来自意大利欧根尼亚山的案例研究
IF 2.5 1区 地球科学 Q1 ANTHROPOLOGY Pub Date : 2026-01-09 DOI: 10.1016/j.jas.2026.106478
Josiah Olah , Cristiano Miele , Matthew Tryc , Filippo Carraro , Simone Dilaria , Jacopo Turchetto , Sebastiano Chiodini , Caterina Previato , Jacopo Bonetto , Michele Secco
This study presents a methodological framework for early-stage archaeological landscape interpretation using light detection and ranging sensors (LiDAR) mounted to an unmanned aerial vehicle (UAV). By integrating high-resolution digital terrain models (DTMs), topographic measurements, k-means clustering, and field validation, the approach moves beyond traditional LiDAR applications for feature detection and towards a usage in preliminary classification and interpretation. Applied to the Euganean Hills in northeastern Italy, a geochemically diverse volcanic region with an extensive history of stone material procurement, this method enabled the identification of 89 potential quarry features, of which 84.9 % of surveyed features were validated as quarries or anthropogenic cuts. Analysis of the region's quarry morphologies and distribution produced several distinct quarry groupings suggestive of phases of exploitation and two case study sites ideal for future provenance research on Euganean volcanic breccias, whose use in Roman construction is known but whose sourced quarries have yet to be identified. Clustering analysis of spatial and morphometric variables indicated the heterogeneity of quarries in the region, differentiated modern and premodern sites, and provided statistical support for the grouping of quarries. These findings demonstrate the interpretive potential of UAV LiDAR in contexts where manned airborne laser scanning (ALS) data is insufficient for confident archaeological analysis and where comprehensive archaeological surveys are impeded by terrain. Beyond this regional case study, the framework offers a high-resolution, cost-effective workflow applicable to rugged, forested landscapes where rapid interpretation of anthropogenic features is needed to inform broader research design like geoarchaeological research or excavation strategies.
本研究提出了一种使用安装在无人驾驶飞行器(UAV)上的光探测和测距传感器(LiDAR)进行早期考古景观解释的方法框架。通过集成高分辨率数字地形模型(dtm)、地形测量、k均值聚类和现场验证,该方法超越了传统的激光雷达特征检测应用,并走向了初步分类和解释的应用。该方法应用于意大利东北部的Euganean Hills,这是一个地球化学多样性的火山地区,具有广泛的石材采掘历史,能够识别89个潜在的采石场特征,其中84.9%的调查特征被证实为采石场或人为切割。对该地区采石场形态和分布的分析产生了几种不同的采石场分组,暗示了开采阶段,以及两个理想的案例研究地点,用于未来对欧陆火山角砾岩的物源研究,其在罗马建筑中的用途是已知的,但其来源采石场尚未确定。空间变量和形态计量变量聚类分析显示了区域内采石场的异质性,区分了现代和前现代遗址,为采石场的分类提供了统计支持。这些发现证明了无人机激光雷达在载人机载激光扫描(ALS)数据不足以进行自信的考古分析和全面考古调查受到地形阻碍的情况下的解释潜力。除了这个区域案例研究之外,该框架还提供了一个高分辨率、经济高效的工作流程,适用于崎岖不平的森林景观,在这些景观中,需要快速解释人为特征,为更广泛的研究设计提供信息,如地质考古研究或挖掘策略。
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引用次数: 0
Detection of rice-based additives in the Forbidden City's historical mortar using DNA barcoding technology 利用DNA条形码技术检测紫禁城历史砂浆中的大米添加剂
IF 2.5 1区 地球科学 Q1 ANTHROPOLOGY Pub Date : 2026-01-09 DOI: 10.1016/j.jas.2026.106476
Qian Wu , Qiong Zhang , Bingjian Zhang , Yulan Hu
The Forbidden City (Imperial Palace), the imperial architectural complex of the Ming and Qing dynasties, has stood for over six centuries since its completion in 1420. The mortar used in its construction—an organic-inorganic composite—played a crucial role in the preservation of these ancient structures. Traditional mortars often incorporated organic additives such as glutinous rice. Was glutinous rice used in the construction of the Forbidden City? Although previous enzyme-linked immunosorbent assay (ELISA) studies detected branched-chain amylopectin additives in the mortar—suggesting the possible use of glutinous rice—conclusive molecular evidence was lacking. However, the identification is complicated by the fact that other starchy plants (e.g., millet) produce similar amylopectin. This challenge is compounded by the geographic context, as Beijing is not a historic cultivation area for glutinous rice. DNA analysis can provide direct molecular evidence to confirm whether glutinous rice or other sticky plants were used. Glutinous rice, exhibits a low-amylose phenotype resulting from a 23 bp insertion in the second exon of the Waxy (Wx) gene. This locus has been confirmed as a key molecular marker for distinguishing between glutinous and non-glutinous varieties.This study aimed to provide direct molecular evidence to determine whether glutinous rice or other sticky plants were used in the mortar of the Forbidden City. Using universal primers, we amplified barcode fragments containing this mutation region via DNA barcode technology. We analyzed the mortar samples collected from Yangxindian and Lingzhao Xuan in the Forbidden City,and the results confirmed showed that glutinous rice was present in five samples from different locations. This indicates that, despite Beijing not being a traditional glutinous rice-producing region, royal construction projects during the Ming and Qing dynasties consistently employed glutinous rice as an organic additive in mortar—a finding consistent with historical records such as the Precedents of the Grand Qing Code.This study provides the first molecular biological evidence confirming the use of glutinous rice in the mortar of the Forbidden City. Accurately identifying the components of historic adhesives helps preserve the original information and value of cultural heritage and supports the principle of “repairing the old as it was” in conservation practice. The findings hold significant value for the restoration and preservation of the ancient architectural complex of the Palace Museum.
紫禁城(故宫)是明清两朝的皇家建筑群,自1420年建成以来,已经矗立了六个多世纪。建造时使用的灰浆是一种有机-无机复合材料,对保存这些古老建筑起了至关重要的作用。传统的砂浆通常加入有机添加剂,如糯米。在紫禁城的建造中使用了糯米吗?虽然以前的酶联免疫吸附试验(ELISA)研究在砂浆中检测到支链支链淀粉添加剂——表明可能使用糯米——但缺乏确凿的分子证据。然而,由于其他淀粉类植物(如小米)也产生类似的支链淀粉,因此鉴定变得复杂。这一挑战与地理环境有关,因为北京并不是糯米的传统种植区。DNA分析可以提供直接的分子证据,以确定是否使用糯米或其他粘性植物。由于糯稻(Wx)基因的第二个外显子插入了23 bp,导致糯稻具有低直链淀粉表型。该基因座已被证实是区分糯和非糯品种的关键分子标记。本研究旨在为确定紫禁城砂浆中是否使用了糯米或其他粘性植物提供直接的分子证据。利用通用引物,通过DNA条形码技术扩增出包含该突变区域的条形码片段。我们分析了紫禁城杨新殿和灵召轩的灰浆样品,结果证实,来自不同地点的五个样品中都含有糯米。这表明,尽管北京不是传统的糯米产区,但明清时期的皇家建筑项目一直使用糯米作为砂浆的有机添加剂,这一发现与《大清法典先例》等历史记录一致。这项研究提供了第一个分子生物学证据,证实了糯米在紫禁城砂浆中的使用。准确识别历史粘合剂的成分,有助于保护文化遗产的原始信息和价值,支持保护实践中“修旧如初”的原则。这一发现对故宫古建筑群的修复和保护具有重要价值。
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引用次数: 0
Seasonality and water source strategies in the Neolithic Near East (ca. 8,000–5,000 BCE): Insights from multi-proxy isotopic and elemental analyses 新石器时代近东地区(约公元前8000 - 5000年)的季节性和水源策略:来自多代同位素和元素分析的见解
IF 2.5 1区 地球科学 Q1 ANTHROPOLOGY Pub Date : 2026-01-06 DOI: 10.1016/j.jas.2025.106462
Janaína N. Ávila , Arkadiusz Sołtysiak , Christine Austin , Hojjat Darabi , Nicole D. Leonard , Hassan Fazeli Nashli , Ian S. Williams , Jian-xin Zhao , Tanya M. Smith
Previous studies have demonstrated that enamel oxygen isotope values (δ18O) reflect rainfall variability experienced by wild primates and ancient humans, providing insights into local climatic conditions. However, it remains less clear whether seasonal patterns can be consistently detected in premodern human communities experiencing different local water dynamics, or how these patterns may vary regionally. Here we present 1958 ion microprobe δ18O measurements linked to enamel growth increments in 15 molars from 13 individuals, representing a combined total of 48 years of enamel formation sampled at approximately weekly resolution. We additionally integrate 87Sr/86Sr and trace-element datasets, contextualized by the δ18O enamel profiles, to assess seasonal adaptation and the likelihood that observed isotopic patterns reflect local rather than non-local signatures. Our dataset spans three Neolithic archaeological sites characterized by distinct water sources: Ali Kosh, Iran (ca. 7,500–6,500 cal BCE); Nemrik 9, Iraq (ca. 8,000–6,500 BCE); and Tepe Sialk North, Iran (ca. 5,800–4,900 BCE). Individuals from Ali Kosh exhibited the highest δ18O values in the dataset along with substantial intra-individual variability, consistent with reliance on evaporatively enriched transient and isotopically diverse water sources—possibly shaped by local mobility or water management strategies. In contrast, individuals from Nemrik 9 displayed clear, large-amplitude annual cycles in their enamel δ18O values, a pattern consistent with a settlement situated near a perennial river subjected to pronounced climatic seasonality. Tepe Sialk North individuals exhibited the lowest δ18O variability, consistent with consumption of isotopically buffered sources such as springs or artesian-fed systems. Predicted drinking water δ18O values for most of the Nemrik 9 and Ali Kosh individuals align well with modern regional rainfall isotope values, while those from Tepe Sialk North underestimate local precipitation δ18O values, as expected for groundwater-fed sources. Additionally, site-specific seasonal patterns were observed in Ba/Ca and Li/Ca elemental ratios, likely shaped by the interplay of regional geology and hydrology. Together, our findings provide valuable insights into Neolithic water access strategies in arid regions, while also underscoring the limitations of identifying bioarchaeological migrants based solely on δ18O values from tooth enamel.
以前的研究表明,牙釉质氧同位素值(δ18O)反映了野生灵长类动物和古代人类经历的降雨变化,为当地气候条件提供了见解。然而,在经历不同地方水动力的前现代人类社区中,是否可以始终检测到季节模式,或者这些模式如何在区域内变化,目前尚不清楚。在这里,我们展示了1958年离子微探针δ18O测量结果,与13个人15磨牙的牙釉质生长增量有关,代表了48年的牙釉质形成取样,大约每周分辨率。此外,我们还整合了87Sr/86Sr和微量元素数据集,并结合δ18O珐琅剖面,以评估季节适应性,以及观察到的同位素模式反映本地而非非本地特征的可能性。我们的数据集涵盖了三个以不同水源为特征的新石器时代考古遗址:伊朗的阿里科什(约公元前7500 - 6500 cal);伊拉克Nemrik 9(约公元前8000 - 6500年);以及伊朗北部的泰佩希尔克(约公元前5800 - 4900年)。来自Ali Kosh的个体在数据集中表现出最高的δ18O值以及大量的个体内部变异性,这与对蒸发富集的瞬态和同位素多样性水源的依赖一致,这可能是由当地流动性或水管理策略形成的。相比之下,Nemrik 9的个体在牙釉质δ18O值上显示出清晰的、大幅度的年循环,这种模式与位于多年生河流附近的定居点一致,受到明显的气候季节性影响。Tepe Sialk North个体表现出最低的δ18O变异,与消耗同位素缓冲源(如弹簧或自流系统)一致。大多数Nemrik 9和Ali Kosh个体的预测饮用水δ18O值与现代区域降雨同位素值吻合良好,而来自Tepe Sialk North的预测低估了当地降水δ18O值,这与对地下水来源的预期一致。此外,Ba/Ca和Li/Ca元素比值的季节变化可能是由区域地质和水文相互作用形成的。总之,我们的研究结果为干旱地区新石器时代的取水策略提供了有价值的见解,同时也强调了仅根据牙釉质的δ18O值识别生物考古移民的局限性。
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Journal of Archaeological Science
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