The China Central Plains in the Tang Dynasty (618–907 CE) was the eastern starting point of the Silk Road, characterized by a long and developed agricultural civilization. It also supported the world's most prosperous city network centered on Chang'an and Luoyang. The use of plant and animal resources by urban residents here offers significant insights into the empire's economy and global interactions. However, direct, quantifiable, and arguably less biased archaeobotanical and zooarchaeological studies are scarce, due to excavation challenges in modern urban areas and inherent limitations in the preservation and identification of macro-remains. Historical documents also tend to record elite groups or accidental events and thus show bias. Here, we analyzed sedimentary ancient DNA (sedaDNA) from an artificial water feature in Zhengzhou Shang Site in a regional city, Guan City (modern-day Zhengzhou), in the Central Plains during the Tang Dynasty. The results show that urban residents used diverse staple food crops, industrial crops, vegetables, fruits and nuts, and cultivated trees and aquatic plants. The staple food crops comprised foxtail millet (Setaria italica) and broomcorn millet (Panicum miliaceum), food legumes, barley (Hordeum vulgare), and wheat (Triticum aestivum); animal resources were mainly for labor, including horses (Equus caballus) and/or donkeys (Equus asinus), dogs (Canis lupus familiaris), and cattle (Bos taurus), with meat consumption minimal or absent. We report the first archaeological evidence for some non-staple crops in the Tang Dynasty, including tea (Camellia sinensis), goji berry (Lycium barbarum), and lotus (Nelumbo nucifera), and others which were introduced into China via the Silk Road, such as spinach (Spinacia oleracea), sesame (Sesamum indicum), pistachio (Pistacia vera), and pomegranate (Punica granatum). Our study provides direct evidence of industrial crop cultivation and non-native crop introduction as mentioned in historical documents, revealing these previously hidden crop cultivation histories and underscoring Tang agriculture's diversity and global connections. Furthermore, DNA translocation in archaeological deposits receives much attention. In this case, comparison between layers and stratigraphic analysis confirmed negligible translocation effects in the upper sediments, although underlying earlier layers show some translocation signals. The sedaDNA provided more taxonomic data than the archaeobotanical macro-remains. The study shows how sedaDNA retrieved from archaeological deposits is beneficial for comprehending the past use of plant and animal resources.
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