{"title":"POLLUTION IN EARLY MODERN KRAKOW","authors":"Rafał Szmytka","doi":"10.2307/j.ctv201xjkz.8","DOIUrl":"https://doi.org/10.2307/j.ctv201xjkz.8","url":null,"abstract":"","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"156 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86326118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE HISTORY OF KRAKOW SMOG","authors":"A. Izdebski, K. Wnęk","doi":"10.2307/j.ctv201xjkz.10","DOIUrl":"https://doi.org/10.2307/j.ctv201xjkz.10","url":null,"abstract":"","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"87 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84106959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE CLIMATE HISTORY OF KRAKOW","authors":"K. Wnęk, A. Izdebski, Leszek Kowanetz","doi":"10.2307/j.ctv201xjkz.4","DOIUrl":"https://doi.org/10.2307/j.ctv201xjkz.4","url":null,"abstract":"","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"19 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89866058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Tian, L. Zhuo, Qing Zhou, Ruoxia Wu, Huang Xiaodi, Z. Liang, Zhen Zhang, Xuefei Tian
Cisplatin (CDDP) has been successfully used in chemotherapy for liver cancer. However, the development of CDDP resistance in HepG2 cells usually leads to relapse and a worsening prognosis. MiR-340-5p has attracted much attention because of its ability to affect cell resistance. This project is intended to explore the role of miR-340-5p and CDK6 in CDDP-R HepG2 cells and provide new ideas for the treatment of liver cancer. A dual-luciferase reporter assay was used to confirm the targeting relationship between miR-340-5p and CDK6. We constructed a CDDP-resistant model of HepG2 cells to examine the effect of miR-340-5p on the drug sensitivity of HepG2 cells. CDDP-R HepG2 cells were transfected with miR-340-5p overexpression plasmid and CDK6 silencing plasmid. QRT-PCR was used to detect the expression of miR-340-5p and CDK6. A western blot was performed to determine the expression of CDK6, CyclinD1, and CyclinD2. CCK-8, flow cytometry, TUNEL and Clonogenic assays were also carried out to detect CDDP-R HepG2 cells. There is a targeting relationship between miR-340-5p and CDK6. The drug resistance of CDDP-R HepG2 cells was significantly higher than that of CDDP-S HepG2 cells. CDDP-R HepG2 cells transfected with both miR-340-5p overexpressing plasmid and CDK6 silencing plasmid showed a lower proliferation ability, cell cycle arrest in the G0/G1 phase, and lower drug resistance compared with single CDDP-R HepG2 cells. Overexpression of miR-340-5p aggravated CDDP-R HepG2 cells' apoptosis and inhibited cell viability. Overexpression of miR-340-5p could reverse the sensitivity of HepG2 cells to CDDP by inhibiting the expression of CDK6 in HepG2 cells.
{"title":"Upregulation of MiR-340-5p Reverses Cisplatin Sensitivity by Inhibiting the Expression of CDK6 in HepG2 Cells","authors":"S. Tian, L. Zhuo, Qing Zhou, Ruoxia Wu, Huang Xiaodi, Z. Liang, Zhen Zhang, Xuefei Tian","doi":"10.3409/FB_69-2.08","DOIUrl":"https://doi.org/10.3409/FB_69-2.08","url":null,"abstract":"Cisplatin (CDDP) has been successfully used in chemotherapy for liver cancer. However, the development of CDDP resistance in HepG2 cells usually leads to relapse and a worsening prognosis. MiR-340-5p has attracted much attention because of its ability to affect cell resistance. This\u0000 project is intended to explore the role of miR-340-5p and CDK6 in CDDP-R HepG2 cells and provide new ideas for the treatment of liver cancer. A dual-luciferase reporter assay was used to confirm the targeting relationship between miR-340-5p and CDK6. We constructed a CDDP-resistant model of\u0000 HepG2 cells to examine the effect of miR-340-5p on the drug sensitivity of HepG2 cells. CDDP-R HepG2 cells were transfected with miR-340-5p overexpression plasmid and CDK6 silencing plasmid. QRT-PCR was used to detect the expression of miR-340-5p and CDK6. A western blot was performed to determine\u0000 the expression of CDK6, CyclinD1, and CyclinD2. CCK-8, flow cytometry, TUNEL and Clonogenic assays were also carried out to detect CDDP-R HepG2 cells. There is a targeting relationship between miR-340-5p and CDK6. The drug resistance of CDDP-R HepG2 cells was significantly higher than that\u0000 of CDDP-S HepG2 cells. CDDP-R HepG2 cells transfected with both miR-340-5p overexpressing plasmid and CDK6 silencing plasmid showed a lower proliferation ability, cell cycle arrest in the G0/G1 phase, and lower drug resistance compared with single CDDP-R HepG2 cells. Overexpression of miR-340-5p\u0000 aggravated CDDP-R HepG2 cells' apoptosis and inhibited cell viability. Overexpression of miR-340-5p could reverse the sensitivity of HepG2 cells to CDDP by inhibiting the expression of CDK6 in HepG2 cells.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48483136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Bojarski, M. Socha, E. Drąg-Kozak, A. Rombel-Bryzek, S. Kapinos, L. Szała, E. Kondera, K. Lugowska, M. Witeska
The values of haematological and selected blood plasma biochemical parameters of juvenile common carp (Cyprinus carpio Linnaeus, 1758) were compared between blood samples taken from caudal vein and heart to evaluate the influence of blood sampling body site on the obtained results in two groups of fish of different blood sampling order: I – first by caudal and then by cardiac puncture, II – first by cardiac and then by caudal puncture. The obtained results revealed statistically significant (p<0.05) differences only in group I where red blood cell (RBC) count was higher in caudal vein blood, while haematocrit (Ht) value, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), total protein (TP) concentration, and magnesium (Mg) level were higher in cardiac blood samples. No statistically significant differences occurred in white blood cell (WBC) count, differential leukocyte count or erythrocyte morphology based on stained blood smears. The obtained results showed that blood sampling body site may affect the results of haematological and plasma biochemical analyses.
{"title":"Does the Site of Blood Collection in Fish Affect Haematological and Blood Biochemical Results?","authors":"B. Bojarski, M. Socha, E. Drąg-Kozak, A. Rombel-Bryzek, S. Kapinos, L. Szała, E. Kondera, K. Lugowska, M. Witeska","doi":"10.3409/FB_69-2.07","DOIUrl":"https://doi.org/10.3409/FB_69-2.07","url":null,"abstract":"The values of haematological and selected blood plasma biochemical parameters of juvenile common carp (Cyprinus carpio Linnaeus, 1758) were compared between blood samples taken from caudal vein and heart to evaluate the influence of blood sampling body site on the obtained results\u0000 in two groups of fish of different blood sampling order: I – first by caudal and then by cardiac puncture, II – first by cardiac and then by caudal puncture. The obtained results revealed statistically significant (p<0.05) differences only in group I where red blood cell (RBC)\u0000 count was higher in caudal vein blood, while haematocrit (Ht) value, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), total protein (TP) concentration, and magnesium (Mg) level were higher in cardiac blood samples. No statistically significant differences occurred in white\u0000 blood cell (WBC) count, differential leukocyte count or erythrocyte morphology based on stained blood smears. The obtained results showed that blood sampling body site may affect the results of haematological and plasma biochemical analyses.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48118756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdominal aortic aneurysm (AAA) is a perilous vascular disease with inflammatory response as its main feature. It is known that the expression of miR-143 is down-regulated in the human aortic aneurysm. In this study, we investigated the effect of miR-143 on AngII-induced VSMCs to learn the potential mechanisms of miR-143 on AAA at the cellular level. The experimental results showed that the expressions of IL-1β, MCP-1, MMP9/13, TLR2, and NF-κB p65 and the percentage of TUNEL-positive cells in AngII-VSMCs were increased significantly compared with the control group. miR-143 had the opposite result. When the expression of miR-143 was up-regulated, the expression of IL-β, MCP-1, and MMP9/13 and the percentage of TUNEL-positive cells in AngII-VSMCs was suppressed. With the transfection of miR-143 over-expression plasmid, IL-1β, MCP-1, and MMP9/13 and the percentage of TUNEL-positive cells were reversed, compared to the AngII group and the AngII+oe-TLR2+miR-143 mimic group. In AngII-induced mouse VSMC, the up-regulation of the miR-143 expression could inactivate the TLR2/NF-κB pathway, thereby alleviating inflammatory response, ECM degradation, and cell apoptosis.
{"title":"MiR-143 Mediates the TLR2/NF-κB Pathway to Attenuate AngII-induced Damage to VSMCs","authors":"Zhenhua Wang, F. Lin, Zhao-ling Cai, G. Lyu","doi":"10.3409/FB_69-2.10","DOIUrl":"https://doi.org/10.3409/FB_69-2.10","url":null,"abstract":"Abdominal aortic aneurysm (AAA) is a perilous vascular disease with inflammatory response as its main feature. It is known that the expression of miR-143 is down-regulated in the human aortic aneurysm. In this study, we investigated the effect of miR-143 on AngII-induced VSMCs to learn\u0000 the potential mechanisms of miR-143 on AAA at the cellular level. The experimental results showed that the expressions of IL-1β, MCP-1, MMP9/13, TLR2, and NF-κB p65 and the percentage of TUNEL-positive cells in AngII-VSMCs were increased significantly compared with the control group.\u0000 miR-143 had the opposite result. When the expression of miR-143 was up-regulated, the expression of IL-β, MCP-1, and MMP9/13 and the percentage of TUNEL-positive cells in AngII-VSMCs was suppressed. With the transfection of miR-143 over-expression plasmid, IL-1β, MCP-1, and MMP9/13\u0000 and the percentage of TUNEL-positive cells were reversed, compared to the AngII group and the AngII+oe-TLR2+miR-143 mimic group. In AngII-induced mouse VSMC, the up-regulation of the miR-143 expression could inactivate the TLR2/NF-κB pathway, thereby alleviating inflammatory response,\u0000 ECM degradation, and cell apoptosis.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41754215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Information on the karyotypes of Turkish species of Cerambycidae is scanty. Our study contributes to the knowledge of the karyological data (chromosomal number and mechanism of sex determination) of five Turkish longicorn beetles; karyotypes of four taxa, one endemic, are described for the first time and for the remaining one, Purpuricenus budensis (Götz, 1783), the previously published chromosome count is confirmed. The chromosome number of Purpuricenus desfontainii inhumeralis Pic, 1891 and Purpuricenus budensis (Götz, 1783) (Cerambycinae, Trachyderini) was found to be 2n = 28 (13 + Xyp); Clytus rhamni Germar, 1817 and Plagionotus floralis (Pallas, 1773) (Cerambycinae, Clytini) 2n = 20 (9 + Xyp); and the endemic Dorcadion triste phrygicum Peks, 1993 (Lamiinae, Dorcadionini) 2n = 24 (11 + Xyp). In view of the paucity of data available until now, our study is important for both to improve the poor karyological knowledge of Turkish Cerambycidae and to provide an incentive for other researchers.
{"title":"New Contribution to the Knowledge on the Chromosome Numbers of Turkish Cerambycidae (Coleoptera)","authors":"Yavuz Kocak, Elmas Yağmur","doi":"10.3409/FB_69-2.11","DOIUrl":"https://doi.org/10.3409/FB_69-2.11","url":null,"abstract":"Information on the karyotypes of Turkish species of Cerambycidae is scanty. Our study contributes to the knowledge of the karyological data (chromosomal number and mechanism of sex determination) of five Turkish longicorn beetles; karyotypes of four taxa, one endemic, are described\u0000 for the first time and for the remaining one, Purpuricenus budensis (Götz, 1783), the previously published chromosome count is confirmed. The chromosome number of Purpuricenus desfontainii inhumeralis Pic, 1891 and Purpuricenus budensis (Götz, 1783) (Cerambycinae,\u0000 Trachyderini) was found to be 2n = 28 (13 + Xyp); Clytus rhamni Germar, 1817 and Plagionotus floralis (Pallas, 1773) (Cerambycinae, Clytini) 2n = 20 (9 + Xyp); and the endemic Dorcadion triste phrygicum Peks, 1993 (Lamiinae, Dorcadionini) 2n = 24\u0000 (11 + Xyp). In view of the paucity of data available until now, our study is important for both to improve the poor karyological knowledge of Turkish Cerambycidae and to provide an incentive for other researchers.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49526718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This review searches for and analyzes existing knowledge on horse tooth anatomy in terms of evolutionary and morphological changes, feeding habits, breeding practices, and welfare. More than 150 articles from relevant databases were analyzed, taking into account the issues of our experimental research on the ultrastructure of Equidae tooth enamel. After our analysis, the knowledge on this subject accumulated up in the past, almost 50 years has been logically arranged into three basic directions: evolutionary-palaeontological, morpho-functional, and dentistic, which is also demonstrated by the latest trends in the study of enamel morphology and in the practice of equine dentistry. The obtained data show that in recent years we have observed a rapid increase in publications and a thematic expansion of the scope of research. It is caused by the need to deepen knowledge in theory and in the practice of feeding species in nature and in captivity as well as the possibility of using new technical resources to improve the excellence of such research. It is a summary of the knowledge of a certain stage of equine tooth enamel studies for this period of time, which serves as the basis for our experimental research (the materials are prepared for publication) and at the same time, defines research perspectives for the next stage of development.
{"title":"Horse Tooth Enamel Ultrastructure: A Review of Evolutionary, Morphological, and Dentistry Approaches","authors":"Vitalii Demeshkant, P. Cwynar, K. Slivinska","doi":"10.3409/FB_69-2.09","DOIUrl":"https://doi.org/10.3409/FB_69-2.09","url":null,"abstract":"This review searches for and analyzes existing knowledge on horse tooth anatomy in terms of evolutionary and morphological changes, feeding habits, breeding practices, and welfare. More than 150 articles from relevant databases were analyzed, taking into account the issues of our experimental\u0000 research on the ultrastructure of Equidae tooth enamel. After our analysis, the knowledge on this subject accumulated up in the past, almost 50 years has been logically arranged into three basic directions: evolutionary-palaeontological, morpho-functional, and dentistic, which is also demonstrated\u0000 by the latest trends in the study of enamel morphology and in the practice of equine dentistry. The obtained data show that in recent years we have observed a rapid increase in publications and a thematic expansion of the scope of research. It is caused by the need to deepen knowledge in theory\u0000 and in the practice of feeding species in nature and in captivity as well as the possibility of using new technical resources to improve the excellence of such research. It is a summary of the knowledge of a certain stage of equine tooth enamel studies for this period of time, which serves\u0000 as the basis for our experimental research (the materials are prepared for publication) and at the same time, defines research perspectives for the next stage of development.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48059692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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