Background: Livestock farming is experiencing a digital transformation and is becoming more information-driven. However, this type of data is often kept in separate storage towers, making it incapable of practicing its potential to boost animal welfare. Lumpy Skin Disease (LSD) is a serious threat to the health of cattle worldwide and has caused financial problems for many cattle farming enterprises. It has been shown that combining machine learning (ML) and artificial intelligence (AI) with biosensor data and conventional visual inspections can improve the identification and diagnosis of this serious condition. Methods: This study presents an extremely precise livestock farming framework that combines data streams from a wide array of disciplines of dairy cattle to see if ever wider/vast data sources enhance the overall projections for diseases and if using the more complicated prediction models can reimburse for less diverse data to some extent. Using images from the farming landscape, this study highlights the utility of convolutional neural networks (CNNs) in the identification of the Lumpy Skin Disease Virus (LSDV) in animals. Result: An analysis of the relative weight given to individual factors in predicting accuracy shows that disease in dairy cattle results from the intricate interactions between many life domains/parameters, such as housing, nutrition and climate; that prediction performance is enhanced by incorporating a wider range of data sources; and that current information can be repurposed to produce useful information for vaccine development. The study highlights the potential of data-driven dairy interventions, focusing on artificial intelligence for disease prediction in cattle, to improve animal welfare and reduce the risk of disease. A Convolutional Neural Network (CNN) based model effectively classified skin conditions with an overall accuracy of 73.89% after 27 training epochs. This study demonstrates CNN’s useful applications in the field of veterinary medicine by highlighting its potential for early detection of Lumpy Skin Disease (LSD).
{"title":"Detection of Disease in Calves using Artificial Intelligence","authors":"A. Alzubi","doi":"10.18805/ijar.bf-1760","DOIUrl":"https://doi.org/10.18805/ijar.bf-1760","url":null,"abstract":"Background: Livestock farming is experiencing a digital transformation and is becoming more information-driven. However, this type of data is often kept in separate storage towers, making it incapable of practicing its potential to boost animal welfare. Lumpy Skin Disease (LSD) is a serious threat to the health of cattle worldwide and has caused financial problems for many cattle farming enterprises. It has been shown that combining machine learning (ML) and artificial intelligence (AI) with biosensor data and conventional visual inspections can improve the identification and diagnosis of this serious condition. Methods: This study presents an extremely precise livestock farming framework that combines data streams from a wide array of disciplines of dairy cattle to see if ever wider/vast data sources enhance the overall projections for diseases and if using the more complicated prediction models can reimburse for less diverse data to some extent. Using images from the farming landscape, this study highlights the utility of convolutional neural networks (CNNs) in the identification of the Lumpy Skin Disease Virus (LSDV) in animals. Result: An analysis of the relative weight given to individual factors in predicting accuracy shows that disease in dairy cattle results from the intricate interactions between many life domains/parameters, such as housing, nutrition and climate; that prediction performance is enhanced by incorporating a wider range of data sources; and that current information can be repurposed to produce useful information for vaccine development. The study highlights the potential of data-driven dairy interventions, focusing on artificial intelligence for disease prediction in cattle, to improve animal welfare and reduce the risk of disease. A Convolutional Neural Network (CNN) based model effectively classified skin conditions with an overall accuracy of 73.89% after 27 training epochs. This study demonstrates CNN’s useful applications in the field of veterinary medicine by highlighting its potential for early detection of Lumpy Skin Disease (LSD).\u0000","PeriodicalId":507727,"journal":{"name":"Indian Journal of Animal Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141831539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Foot-and-mouth disease (FMD) is a widespread and contagious disease affecting various animal species. The effectiveness of control tools is impeded by the high costs associated with animal experimentation, an incomplete understanding of host immune systems and a scarcity of immunological reagents. 99mTc radiopharmaceuticals, known for their versatile chemistry, play a crucial role in preclinical research and drug development. The objective of the present study was to develop nuclear medicine-enabled strategies for custom designing and uplifting engineered vaccines, specifically nanoparticle vaccines, in guinea pigs. Methods: In the present study, we elucidate the technique used for direct radiolabelling of the FMD vaccine and its subsequent distribution within guinea pigs. The study involved the evaluation of 99mTc-radiolabeling and quality control, as well as the disposition of the 99mTc-FMD vaccine in guinea pigs. Result: Radiolabelling and stability studies showed 95.00% efficiency and 92.93% stability. The study demonstrated that the 99mTc-FMD vaccine showed increased uptake and delayed clearance from lymphoid organs, particularly the spleen and prescapular lymph node, compared to plain 99mTc.
{"title":"Studies on the Disposition of Technetium-99m Labeled Foot-and-mouth Disease Vaccine in Guinea Pigs","authors":"C. Galdhar, Anuj Kakade, J.R. Kawade, P.M. Sapkal, Mahesh Shinde, Vishal Mote, Sandeep Patankar, B.P. Sreenivasa","doi":"10.18805/ijar.b-5287","DOIUrl":"https://doi.org/10.18805/ijar.b-5287","url":null,"abstract":"Background: Foot-and-mouth disease (FMD) is a widespread and contagious disease affecting various animal species. The effectiveness of control tools is impeded by the high costs associated with animal experimentation, an incomplete understanding of host immune systems and a scarcity of immunological reagents. 99mTc radiopharmaceuticals, known for their versatile chemistry, play a crucial role in preclinical research and drug development. The objective of the present study was to develop nuclear medicine-enabled strategies for custom designing and uplifting engineered vaccines, specifically nanoparticle vaccines, in guinea pigs. Methods: In the present study, we elucidate the technique used for direct radiolabelling of the FMD vaccine and its subsequent distribution within guinea pigs. The study involved the evaluation of 99mTc-radiolabeling and quality control, as well as the disposition of the 99mTc-FMD vaccine in guinea pigs. Result: Radiolabelling and stability studies showed 95.00% efficiency and 92.93% stability. The study demonstrated that the 99mTc-FMD vaccine showed increased uptake and delayed clearance from lymphoid organs, particularly the spleen and prescapular lymph node, compared to plain 99mTc.\u0000","PeriodicalId":507727,"journal":{"name":"Indian Journal of Animal Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141642825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The human skin is a natural barrier that protects against external stress, but its physiological structure can be compromised when it gets damaged. In the field of medical science, wounds are a significant issue that requires immediate attention. Lavandula species are used in the food, perfume and cosmetics industries due to their antimicrobial, anti-inflammatory and wound-healing properties. Methods: The present study was done to assess the biocompatibility, antimicrobial and wound-healing potential of L. dentata leave methanolic extract (LE) that is cultivated in the Taif region of Saudi Arabia. The biocompatibility of LE was analyzed against the normal human skin fibroblast (HSF) cell line using SRB assay. A quarter of LE IC50 was used for wound healing assay. Finally, Escherichia Coli, Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus pyogenes were used to determine LE antibacterial potential through the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) measurement. Result: LE appears safe on HSF cell line with IC50 = 97 mg/mL. LE decreased wound width, wound area and the mean cell migratory rate of wound border cells during scratch closure, while increased the wound closure% in a time-dependent manner compared to negative control. LE shows antimicrobial potential against all four strains at MIC and MBC greater than 3.0 mg/mL, except for Streptococcus pyogenes MIC= 3.0 mg/mL. In conclusion, LE offers therapeutic benefits against wound healing with an antimicrobial effect, however, higher concentrations of LE should be used to assess its effect.
背景：人体皮肤是抵御外界压力的天然屏障，但皮肤一旦受损，其生理结构就会受到损害。在医学领域，伤口是一个需要立即关注的重要问题。薰衣草具有抗菌、消炎和伤口愈合的特性，因此被用于食品、香水和化妆品行业。研究方法本研究旨在评估在沙特阿拉伯塔伊夫地区栽培的 L. dentata 叶甲醇提取物（LE）的生物相容性、抗菌性和伤口愈合潜力。使用 SRB 分析法分析了牙鲆叶甲醇提取物对正常人皮肤成纤维细胞（HSF）的生物相容性。伤口愈合试验使用了四分之一的 LE IC50。最后，使用大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌和化脓性链球菌，通过测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）来确定 LE 的抗菌潜力。结果：LE 对 HSF 细胞系是安全的，IC50 = 97 mg/mL。与阴性对照组相比，LE能减少划痕闭合过程中的伤口宽度、伤口面积和伤口边缘细胞的平均迁移率，同时以时间依赖性的方式提高伤口闭合率。除化脓性链球菌的 MIC= 3.0 mg/mL 外，LE 对所有四种菌株的 MIC 和 MBC 均大于 3.0 mg/mL，显示出抗菌潜力。总之，LE 具有抗菌作用，可促进伤口愈合，但应使用更高浓度的 LE 来评估其效果。
{"title":"Biocompatibility, Antimicrobial and Wound Healing Activities of Lavandula dentata Leaves Extract","authors":"N. Hussien, Hanan Ramadan Hamad Mohamed","doi":"10.18805/ijar.bf-1814","DOIUrl":"https://doi.org/10.18805/ijar.bf-1814","url":null,"abstract":"Background: The human skin is a natural barrier that protects against external stress, but its physiological structure can be compromised when it gets damaged. In the field of medical science, wounds are a significant issue that requires immediate attention. Lavandula species are used in the food, perfume and cosmetics industries due to their antimicrobial, anti-inflammatory and wound-healing properties. Methods: The present study was done to assess the biocompatibility, antimicrobial and wound-healing potential of L. dentata leave methanolic extract (LE) that is cultivated in the Taif region of Saudi Arabia. The biocompatibility of LE was analyzed against the normal human skin fibroblast (HSF) cell line using SRB assay. A quarter of LE IC50 was used for wound healing assay. Finally, Escherichia Coli, Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus pyogenes were used to determine LE antibacterial potential through the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) measurement. Result: LE appears safe on HSF cell line with IC50 = 97 mg/mL. LE decreased wound width, wound area and the mean cell migratory rate of wound border cells during scratch closure, while increased the wound closure% in a time-dependent manner compared to negative control. LE shows antimicrobial potential against all four strains at MIC and MBC greater than 3.0 mg/mL, except for Streptococcus pyogenes MIC= 3.0 mg/mL. In conclusion, LE offers therapeutic benefits against wound healing with an antimicrobial effect, however, higher concentrations of LE should be used to assess its effect.\u0000","PeriodicalId":507727,"journal":{"name":"Indian Journal of Animal Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141648175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guang-ping Ruan, Feng Yan, Xiang Yao, Jing Gao, Xiang-Yu Feng, Tao Ye, Xing-Hua Pan
Background: Natural killer (NK) cells are an important part of the immune system and play a central role in cell-mediated immune responses. Recent studies have shown that NK cells promote organ rejuvenation, thus achieving anti-aging effects from the inside to out. Methods: We amplified and cultured human peripheral blood NK cells and the positive rate reached 43.91%. After purification, the positive rate reached 92.65%. The cultured NK showed a good killing effect on tumor cells and the NK cells reached the standard preparation scale. The NK cells cultured by us were co-cultured with the PBMC of aging macaques and the quantitative PCR and WB results showed that the expression of aging gene and aging protein of the PBMC of aging macaques decreased significantly after co-culture, with statistical significance (P less than 0.01). Result: These results indicate that NK cells prepared with standard preparation have anti-aging effect, which will have extensive significance in preclinical research.
背景：自然杀伤（NK）细胞是免疫系统的重要组成部分，在细胞介导的免疫反应中发挥着核心作用。最新研究表明，NK 细胞能促进器官年轻化，从而达到由内而外的抗衰老效果。研究方法我们扩增并培养了人类外周血 NK 细胞，阳性率达到 43.91%。纯化后，阳性率达到 92.65%。培养出的 NK 对肿瘤细胞有很好的杀伤作用，达到了标准制备规模。我们将培养的 NK 细胞与衰老猕猴的 PBMC 共培养，定量 PCR 和 WB 结果显示，共培养后衰老猕猴 PBMC 的衰老基因和衰老蛋白的表达量明显下降，差异有统计学意义（P 小于 0.01）。结果这些结果表明，用标准制剂制备的 NK 细胞具有抗衰老作用，这将在临床前研究中具有广泛的意义。
{"title":"Study on the Standardized Preparation of NK Cells and the Mechanism of Anti-aging of Rhesus Monkey PBMC","authors":"Guang-ping Ruan, Feng Yan, Xiang Yao, Jing Gao, Xiang-Yu Feng, Tao Ye, Xing-Hua Pan","doi":"10.18805/ijar.bf-1779","DOIUrl":"https://doi.org/10.18805/ijar.bf-1779","url":null,"abstract":"Background: Natural killer (NK) cells are an important part of the immune system and play a central role in cell-mediated immune responses. Recent studies have shown that NK cells promote organ rejuvenation, thus achieving anti-aging effects from the inside to out. Methods: We amplified and cultured human peripheral blood NK cells and the positive rate reached 43.91%. After purification, the positive rate reached 92.65%. The cultured NK showed a good killing effect on tumor cells and the NK cells reached the standard preparation scale. The NK cells cultured by us were co-cultured with the PBMC of aging macaques and the quantitative PCR and WB results showed that the expression of aging gene and aging protein of the PBMC of aging macaques decreased significantly after co-culture, with statistical significance (P less than 0.01). Result: These results indicate that NK cells prepared with standard preparation have anti-aging effect, which will have extensive significance in preclinical research.\u0000","PeriodicalId":507727,"journal":{"name":"Indian Journal of Animal Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141646019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Rabbits are the most important protein sources of animals for humans all over the world. Its production has increased significantly in recent years and it has become one of the most important animal sources in the Kingdom of Saudi Arabia. Coccidiosis is one of the most dangerous diseases affecting rabbits and other animals causing global economic losses. Plant extracts have been used as potential alternatives to chemotherapy because they do not have the negative consequences of tissue deposits and drug resistance. Methods: The current study examined the antioxidant activity of Vitis vinifera leaf extract (VVLE) in New Zealand rabbits infected with Eimeria stiedae. The extract was subjected to phytochemical analysis by FT-IR. A total of 35 male New Zealand rabbits were divided into seven groups. Group 1 served as the uninfected, untreated (negative control) group. Group 2 uninfected and treated at 400 mg/kg of extract to test for toxicity. Group 3 represented the infected, non-treated (positive control) group. While, groups 4, 5 and 6 comprised infected treated with at100 mg/kg, 200 mg/kg and 400 mg/kg of extracts, respectively. Finally, group 7 consisted of infected rabbits treated with Toltrazuril at a dosage of 5 mg/kg body weight. Result: The analysis of phytochemicals by FT-IR for Vitis vinifera leaf extract revealed the existence of 15 biologically active compounds. VVLE was able to decrease the induced weight loss due to infection. Moreover, the oxidative status due to E. stiedae infection had been changed after treatment with VVLE where the level of glutathione, malondialdehyde and nitric oxide was improved indicating the antioxidant activity of the VVLE. Our findings suggested that VVLE could boost the induced oxidative stress in the liver of infected New Zealand rabbits, but studies are needed to determine the mechanism of VVLE.
{"title":"Effects Vitis vinifera Leaves on the Oxidative Status of New Zealand Rabbit Liver Infected with Eimeria stiedae","authors":"M. Mares, R. Abdel-Gaber, S. Al-quraishy","doi":"10.18805/ijar.bf-1805","DOIUrl":"https://doi.org/10.18805/ijar.bf-1805","url":null,"abstract":"Background: Rabbits are the most important protein sources of animals for humans all over the world. Its production has increased significantly in recent years and it has become one of the most important animal sources in the Kingdom of Saudi Arabia. Coccidiosis is one of the most dangerous diseases affecting rabbits and other animals causing global economic losses. Plant extracts have been used as potential alternatives to chemotherapy because they do not have the negative consequences of tissue deposits and drug resistance. Methods: The current study examined the antioxidant activity of Vitis vinifera leaf extract (VVLE) in New Zealand rabbits infected with Eimeria stiedae. The extract was subjected to phytochemical analysis by FT-IR. A total of 35 male New Zealand rabbits were divided into seven groups. Group 1 served as the uninfected, untreated (negative control) group. Group 2 uninfected and treated at 400 mg/kg of extract to test for toxicity. Group 3 represented the infected, non-treated (positive control) group. While, groups 4, 5 and 6 comprised infected treated with at100 mg/kg, 200 mg/kg and 400 mg/kg of extracts, respectively. Finally, group 7 consisted of infected rabbits treated with Toltrazuril at a dosage of 5 mg/kg body weight. Result: The analysis of phytochemicals by FT-IR for Vitis vinifera leaf extract revealed the existence of 15 biologically active compounds. VVLE was able to decrease the induced weight loss due to infection. Moreover, the oxidative status due to E. stiedae infection had been changed after treatment with VVLE where the level of glutathione, malondialdehyde and nitric oxide was improved indicating the antioxidant activity of the VVLE. Our findings suggested that VVLE could boost the induced oxidative stress in the liver of infected New Zealand rabbits, but studies are needed to determine the mechanism of VVLE.\u0000","PeriodicalId":507727,"journal":{"name":"Indian Journal of Animal Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141646864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Behera, R. Ravindran, R. S. Arya, P. Behera, G. Patra, S.J. Islam, R. Sharma, B. Sahoo, M. Mohanta
Background: Bovines are domesticated ungulates mainly raised as livestock for milk, meat and leather and as draught animals. A slaughterhouse study helps to assess the disease status of herds and contains valuable information about the incidence and epidemiology of animal diseases. Haemoprotozoan diseases are one of the major problems which adversely affect the health and productivity of cattle. The diverse climatic zones of India are highly conducive to the survival and propagation of vectors and vector-borne pathogens. However, there is no data on proper slaughterhouse study of bovines in Mizoram even in India for determination of the prevalence of haemoprotozoan infections. Methods: The blood samples were collected in an EDTA vials. The blood smear was prepared, stained with Giemsa stain and examined for the presence of haemoprotozoan. The DNA was isolated from positive samples by use of a DNeasy Blood and Tissue Kit. Then PCR was performed for all the isolated DNA collected from positive samples. After confirmation through PCR, the representative samples for Anaplasma sp. and Theileria sp. were sent for sequencing. Then the sequencing results were annotated using BLAST, ClustalW multiple alignment program of MEGA9 software. The phylogenetic tree was generated by using the Neighbour-Joining method, keeping bootstrap consensus from 1000 replicates. Result: After the blood smear examination, PCR was performed for confirmation. Molecular detection of Anaplasma marginale, Theileria orientalis was done. Molecular characterization of 2 isolates from Anaplasma marginale and Theileria orientalis was performed by sequencing. The sequence analysis of rpoB gene of isolates of Anaplasma revealed both the isolates were placed in the same clade in the phylogenetic tree. The phylogenetic analysis of Thileria isolates revealed that isolate Miz-86 was closely placed with the Type-N3 clade. Whereas the other isolate i.e. Miz-87, belonged to the Type-5 clade.
{"title":"Molecular Characterisation of Anaplasma marginale and Theileria orientalis in Slaughtered Bovines of Aizawl District, Mizoram","authors":"B. Behera, R. Ravindran, R. S. Arya, P. Behera, G. Patra, S.J. Islam, R. Sharma, B. Sahoo, M. Mohanta","doi":"10.18805/ijar.b-5255","DOIUrl":"https://doi.org/10.18805/ijar.b-5255","url":null,"abstract":"Background: Bovines are domesticated ungulates mainly raised as livestock for milk, meat and leather and as draught animals. A slaughterhouse study helps to assess the disease status of herds and contains valuable information about the incidence and epidemiology of animal diseases. Haemoprotozoan diseases are one of the major problems which adversely affect the health and productivity of cattle. The diverse climatic zones of India are highly conducive to the survival and propagation of vectors and vector-borne pathogens. However, there is no data on proper slaughterhouse study of bovines in Mizoram even in India for determination of the prevalence of haemoprotozoan infections. Methods: The blood samples were collected in an EDTA vials. The blood smear was prepared, stained with Giemsa stain and examined for the presence of haemoprotozoan. The DNA was isolated from positive samples by use of a DNeasy Blood and Tissue Kit. Then PCR was performed for all the isolated DNA collected from positive samples. After confirmation through PCR, the representative samples for Anaplasma sp. and Theileria sp. were sent for sequencing. Then the sequencing results were annotated using BLAST, ClustalW multiple alignment program of MEGA9 software. The phylogenetic tree was generated by using the Neighbour-Joining method, keeping bootstrap consensus from 1000 replicates. Result: After the blood smear examination, PCR was performed for confirmation. Molecular detection of Anaplasma marginale, Theileria orientalis was done. Molecular characterization of 2 isolates from Anaplasma marginale and Theileria orientalis was performed by sequencing. The sequence analysis of rpoB gene of isolates of Anaplasma revealed both the isolates were placed in the same clade in the phylogenetic tree. The phylogenetic analysis of Thileria isolates revealed that isolate Miz-86 was closely placed with the Type-N3 clade. Whereas the other isolate i.e. Miz-87, belonged to the Type-5 clade.\u0000","PeriodicalId":507727,"journal":{"name":"Indian Journal of Animal Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141650628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: This study was aimed to assess the potential renoprotective properties of broccoli extract in pre-pubertal Wistar rats subjected to lead acetate-induced renal toxicity. Lead acetate poses a significant health threat due to its toxic effects on various organ systems, particularly the kidneys, which play a crucial role in waste elimination and maintaining physiological balance. Methods: The study design involves the administration of lead acetate to induce subchronic intoxication in experimental rats, alongside treatment with broccoli extract and vitamin E to assess their protective effects. Result: Biochemical analyses reveal significant increases in urea, uric acid, creatinine and BUN levels in the lead acetate-treated group, indicative of impaired renal function. However, treatment with broccoli extract demonstrates a significant reduction in these biomarkers, comparable to the effects observed with vitamin E treatment, suggesting potential renoprotective benefits. Histopathological examination corroborates these findings, with the lead acetate group exhibiting severe renal damage, including degeneration, necrosis and inflammatory cell infiltration, while broccoli extract treatment partially mitigates these effects. The study highlights the importance of natural compounds like broccoli in combating heavy metal-induced renal toxicity and underscores the need for further investigation into their protective mechanisms. These findings hold implications for the development of preventive strategies and therapeutic interventions against lead-induced nephrotoxicity, offering valuable insights into the potential of broccoli extract as a natural renoprotective agent.
研究背景本研究旨在评估西兰花提取物对青春期前Wistar大鼠的潜在肾脏保护作用。醋酸铅对各器官系统都有毒性作用，尤其是对肾脏，因为肾脏在排泄废物和维持生理平衡方面起着至关重要的作用。研究方法研究设计包括给实验鼠施用醋酸铅诱导亚慢性中毒，同时用西兰花提取物和维生素 E 治疗，以评估它们的保护作用。研究结果生化分析表明，醋酸铅处理组的尿素、尿酸、肌酐和尿素氮水平明显升高，表明肾功能受损。然而，使用西兰花提取物治疗后，这些生物标志物明显减少，与维生素 E 治疗的效果相当，这表明西兰花提取物具有潜在的肾脏保护作用。组织病理学检查证实了这些发现，醋酸铅组表现出严重的肾损伤，包括变性、坏死和炎症细胞浸润，而西兰花提取物治疗则部分减轻了这些影响。这些发现对开发针对铅诱导的肾毒性的预防策略和治疗干预措施具有重要意义，并为西兰花提取物作为一种天然肾保护剂的潜力提供了宝贵的见解。
{"title":"Renoprotective Effect of Broccoli Extract in Pre-pubertal Wistar Rats Exposed to Lead Acetate Toxicity","authors":"Nithya Venugopal, Murali Punniakotti, G. Ramanujam, Abarajitha Shankara Narayanan, Shyamaladevi Babu, Radhika Jayaraman","doi":"10.18805/ijar.b-5354","DOIUrl":"https://doi.org/10.18805/ijar.b-5354","url":null,"abstract":"Background: This study was aimed to assess the potential renoprotective properties of broccoli extract in pre-pubertal Wistar rats subjected to lead acetate-induced renal toxicity. Lead acetate poses a significant health threat due to its toxic effects on various organ systems, particularly the kidneys, which play a crucial role in waste elimination and maintaining physiological balance. Methods: The study design involves the administration of lead acetate to induce subchronic intoxication in experimental rats, alongside treatment with broccoli extract and vitamin E to assess their protective effects. Result: Biochemical analyses reveal significant increases in urea, uric acid, creatinine and BUN levels in the lead acetate-treated group, indicative of impaired renal function. However, treatment with broccoli extract demonstrates a significant reduction in these biomarkers, comparable to the effects observed with vitamin E treatment, suggesting potential renoprotective benefits. Histopathological examination corroborates these findings, with the lead acetate group exhibiting severe renal damage, including degeneration, necrosis and inflammatory cell infiltration, while broccoli extract treatment partially mitigates these effects.\u0000The study highlights the importance of natural compounds like broccoli in combating heavy metal-induced renal toxicity and underscores the need for further investigation into their protective mechanisms. These findings hold implications for the development of preventive strategies and therapeutic interventions against lead-induced nephrotoxicity, offering valuable insights into the potential of broccoli extract as a natural renoprotective agent.\u0000","PeriodicalId":507727,"journal":{"name":"Indian Journal of Animal Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141653459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Shitikova, M. Atroshchenko, V. Zvyagina, E. Belskikh, A.I. Romanova
Background: The aim of this work is to study the significance of carnitine fractions and NO metabolites for the assessment of spermatozoa resistance to cryopreservation, for which purpose these parameters were investigated in seminal plasma of animals divided according to the indices of spermatozoa of stallions subjected to cryopreservation. Methods: There were 18 breeding stallions with an average age of 11.28±5.46 years, all 18 were of Arabian breed. Using cluster analysis of indicators of semen quality, animals were identified into two groups: with high and low sperm resistance to cryopreservation-thawing. We assessed standard quality parameters in fresh and cryopreserved sperm, as well as carnitine and its fractions and NO metabolites in stallions’ seminal plasma, steroid hormones level and carnitine fractions in blood serum. Data were analyzed using JASP. Differences at p less than 0.05 were considered statistically significant. Result: Seminal plasma obtained from animals from the cluster with low motility after cryopreservation-thawing was characterized by a lower concentration of nitric oxide metabolites in 1,79 time (p=0.0415) and free carnitine in 1,64 time (p=0.0349). Positive moderate strength correlation were established between the level of seminal plasma free carnitine and the percentage of live spermatozoa after sperm cryopreservation-thawing.
背景：这项工作的目的是研究肉碱组分和 NO 代谢物对评估精子抗冷冻能力的意义，为此研究了根据接受冷冻保存的种公马精子指数划分的动物精浆中的这些参数。研究方法18匹种公马的平均年龄为（11.28±5.46）岁，均为阿拉伯种。通过对精液质量指标进行聚类分析，将动物分为两组：精子对冷冻解冻的耐受性高和耐受性低。我们评估了新鲜精子和冷冻保存精子的标准质量参数，以及种公马精浆中的肉碱及其组分和氮氧化物代谢物、血清中的类固醇激素水平和肉碱组分。数据使用 JASP 进行分析。P小于0.05的差异被认为具有统计学意义。结果从低温解冻后运动能力低的组群中获得的精浆的特点是，一氧化氮代谢物的浓度在1.79倍时较低（p=0.0415），游离肉碱的浓度在1.64倍时较低（p=0.0349）。精子冷冻-解冻后，精浆游离肉碱水平与活精子百分比之间呈中度正相关。
{"title":"Importance of L-carnitine as Biochemical Marker for Semen Quality Preservation after Cryopreservation in Stallions","authors":"A. Shitikova, M. Atroshchenko, V. Zvyagina, E. Belskikh, A.I. Romanova","doi":"10.18805/ijar.bf-1792","DOIUrl":"https://doi.org/10.18805/ijar.bf-1792","url":null,"abstract":"Background: The aim of this work is to study the significance of carnitine fractions and NO metabolites for the assessment of spermatozoa resistance to cryopreservation, for which purpose these parameters were investigated in seminal plasma of animals divided according to the indices of spermatozoa of stallions subjected to cryopreservation. Methods: There were 18 breeding stallions with an average age of 11.28±5.46 years, all 18 were of Arabian breed. Using cluster analysis of indicators of semen quality, animals were identified into two groups: with high and low sperm resistance to cryopreservation-thawing. We assessed standard quality parameters in fresh and cryopreserved sperm, as well as carnitine and its fractions and NO metabolites in stallions’ seminal plasma, steroid hormones level and carnitine fractions in blood serum. Data were analyzed using JASP. Differences at p less than 0.05 were considered statistically significant. Result: Seminal plasma obtained from animals from the cluster with low motility after cryopreservation-thawing was characterized by a lower concentration of nitric oxide metabolites in 1,79 time (p=0.0415) and free carnitine in 1,64 time (p=0.0349). Positive moderate strength correlation were established between the level of seminal plasma free carnitine and the percentage of live spermatozoa after sperm cryopreservation-thawing.\u0000","PeriodicalId":507727,"journal":{"name":"Indian Journal of Animal Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141665612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Audarya, Y. Chatur, A. K. Niranjan, D. Chhabra, R. Gangil, R. Sharda, R. Aich, A.K. Mishra
Background: Brucellosis is one of India’s most important bacterial diseases of large ruminants. It is of economic importance. It is caused by members of the Brucella species and responsible for abortion in pregnant bovines usually during the first term and generally after the fifth month of gestation. Hence, livestock farm with a history of abortion in pregnant animals has to be investigated for Brucellosis. Methods: Whole blood samples (of 50 individual buffaloes) were collected aseptically from a herd of buffaloes experiencing a natural outbreak of Brucellosis. Extracted serum samples were tested after clarification in commercially procured competitive enzyme-linked immunosorbent assay for the serological laboratory diagnosis of Brucellosis. Result: Out of 50 buffalo serum samples tested by using commercially procured competitive enzyme-linked immunosorbent assay, a total of 24 buffalo serum samples tested positive for the presence of Antibodies against Brucella species. Overall seropositivity of 48% was recorded for Brucellosis in the investigated buffalo herd experiencing a natural infection in the present study even after long-term storage of serum samples at deep freezing conditions.
{"title":"Status of Infection during a Natural Outbreak of Brucellosis in Unvaccinated Buffaloes at a Livestock Farm of Madhya Pradesh in India by using Competitive Enzyme-linked Immunosorbent Assay","authors":"S. Audarya, Y. Chatur, A. K. Niranjan, D. Chhabra, R. Gangil, R. Sharda, R. Aich, A.K. Mishra","doi":"10.18805/ijar.b-5197","DOIUrl":"https://doi.org/10.18805/ijar.b-5197","url":null,"abstract":"Background: Brucellosis is one of India’s most important bacterial diseases of large ruminants. It is of economic importance. It is caused by members of the Brucella species and responsible for abortion in pregnant bovines usually during the first term and generally after the fifth month of gestation. Hence, livestock farm with a history of abortion in pregnant animals has to be investigated for Brucellosis. Methods: Whole blood samples (of 50 individual buffaloes) were collected aseptically from a herd of buffaloes experiencing a natural outbreak of Brucellosis. Extracted serum samples were tested after clarification in commercially procured competitive enzyme-linked immunosorbent assay for the serological laboratory diagnosis of Brucellosis. Result: Out of 50 buffalo serum samples tested by using commercially procured competitive enzyme-linked immunosorbent assay, a total of 24 buffalo serum samples tested positive for the presence of Antibodies against Brucella species. Overall seropositivity of 48% was recorded for Brucellosis in the investigated buffalo herd experiencing a natural infection in the present study even after long-term storage of serum samples at deep freezing conditions.\u0000","PeriodicalId":507727,"journal":{"name":"Indian Journal of Animal Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141668715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. S. Azad, J. K. Prasad, Anil Kumar, D. Sengupta, S. K. Sheetal, Ankesh Kumar, Ramesh Kumar Nirala, Avnish Kumar Gautam
Background: The Bachaur is a medium sized draft purpose breed mostly present in Madhubani, Darbhanga and Sitamarhi districts of Bihar and presently the breed is at the verge of extinction. Since no data is presently available regarding the estrus pattern in these cows therefore, present study was designed to document the oestrus intensity, estrus cycle length, estrus behaviour and duration of estrus in these animals. Methods: The total of nine healthy breeding Bachaur cows aged 3 to 5 years were maintained under identical managemental conditions.The estrous cycles of nine animals was synchronized using Cloprostenol Sodium @ 500 mg I/M (Pragma®, Intas, India) in cycling Bachaur cows. Estrus was detected in the morning and evening for external signs of estrus, estrus behavior, intensity of estrus, estrus duration and estrous cycle length. Result: In the present study, mounting or mounted by other animals was observed in more than 70% and sniffing, tried to lick and Flehmen’s reaction was observed in about 66.67% of treated cows. The other parameters like bellowing, restlessness, milk yield reduction, frequent micturition and Flehmen’s reaction were recorded in 44.44%, 55.56%, 44.44%, 55.56% and 66.67%, of animals, respectively. The most appreciable physical sign of estrus recorded was intense swelling of vulva (in 88.89%) however hyperaemia of vulva, copious estrual mucous discharge, pronounced tonicity of uterus, completely dilated os cervix and presence of large palpable follicle on the ovary was observed in 44.44%, 55.56%, 66.67%, 77.78% and 66.67%, of cows respectively.
{"title":"Reproductive Performance of Estrus Pattern in Bachaur Cows of Bihar","authors":"C. S. Azad, J. K. Prasad, Anil Kumar, D. Sengupta, S. K. Sheetal, Ankesh Kumar, Ramesh Kumar Nirala, Avnish Kumar Gautam","doi":"10.18805/ijar.b-5361","DOIUrl":"https://doi.org/10.18805/ijar.b-5361","url":null,"abstract":"Background: The Bachaur is a medium sized draft purpose breed mostly present in Madhubani, Darbhanga and Sitamarhi districts of Bihar and presently the breed is at the verge of extinction. Since no data is presently available regarding the estrus pattern in these cows therefore, present study was designed to document the oestrus intensity, estrus cycle length, estrus behaviour and duration of estrus in these animals. Methods: The total of nine healthy breeding Bachaur cows aged 3 to 5 years were maintained under identical managemental conditions.The estrous cycles of nine animals was synchronized using Cloprostenol Sodium @ 500 mg I/M (Pragma®, Intas, India) in cycling Bachaur cows. Estrus was detected in the morning and evening for external signs of estrus, estrus behavior, intensity of estrus, estrus duration and estrous cycle length. Result: In the present study, mounting or mounted by other animals was observed in more than 70% and sniffing, tried to lick and Flehmen’s reaction was observed in about 66.67% of treated cows. The other parameters like bellowing, restlessness, milk yield reduction, frequent micturition and Flehmen’s reaction were recorded in 44.44%, 55.56%, 44.44%, 55.56% and 66.67%, of animals, respectively. The most appreciable physical sign of estrus recorded was intense swelling of vulva (in 88.89%) however hyperaemia of vulva, copious estrual mucous discharge, pronounced tonicity of uterus, completely dilated os cervix and presence of large palpable follicle on the ovary was observed in 44.44%, 55.56%, 66.67%, 77.78% and 66.67%, of cows respectively.","PeriodicalId":507727,"journal":{"name":"Indian Journal of Animal Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141669993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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