Pub Date : 2020-08-24DOI: 10.3727/036012920x15958782196790
Winston I. Lu, D. Lu
The purpose of this research was to proce or disprove the widely held beliefs that X-ray radiation used in medical or dental applications may affect the normal oral flora, and may also have effects on the micro-organisms existing in the oral cavity of every person. Such beliefs might be due to the common knowledge that radiation therapy has been utilized for the long time in the cancer patients to destroy the cancerous cells, and that radiation are also widely used in agriculture to prolong the shelf life of the farm products by destroying the decay-causing microorganisms existing in the agricultural products such as meats, produce, etc. Since very few research has been conducted in this concerned area, and not much pertinent information in the scientific literature could be found in this regard, a research experiment was set up to find out if these common beliefs have any merits. The research experiment involved selecting BDORT(By-digital O-Ring Test) positive dishes to incubate human saliva collected from a volunteer patient. Once dish served as control which was not subjected X-ray irradiation. Other dishes were subjected X-ray irradiation with various doses of irradiation strength at various time interval such as 1, 3, 5, 7, and 9 seconds to find out if any significant change taken place in the bacteria colonies. The bacterial colonies in the irradiated dishes then compared with that of the control dish so as to draw a conclusion if radiation for medical and dental X-ray machines would actually have any bactericidal or/and bacteriostatic effects on the oral micro-organisms flora. Finally, dishes were brought to a local hospital Radiology Department to use high dose of irradaition (used for cancer therapy) on the dishes to find out to what extent if such a high dose X-ray irradiation emitted from therapeutic cancer therapy machine would affect the number of bacterial colonies in the dishes as when compared with when dishes were under low doses of X-ray irradiation from dental or medical diagnostic X-ray machines. During the experiment, dishes, when irradiated, were sent to a certified medical technologist in the hospital laboratory to count the number of the bacterial colonies, and each change in the bacterial colony number were recorded in to data for biostatic analysis. The data were compared with BDORT negativity scores collected each time before and after dishes were irradiated, thus to find out if any corresponding confirmity between traditional laboratory findings and the score changes from BDORT findings. The results of this experiment revealed the fact that radiation from regular diagnostic X-ray machines possess little effect on bacteria, and that they have little effect on both cells and bacteria present in the human body. Neverthless, dramatically increasing the high radiation dosages does have potential to both inhibit and destroy bacteria. Therefore,using irradiation techniques to inhibit bacterial growth is only useful
{"title":"The Bacteriostatic and Bactericidal Effects of Radiation from Dental and Medical X-Rays","authors":"Winston I. Lu, D. Lu","doi":"10.3727/036012920x15958782196790","DOIUrl":"https://doi.org/10.3727/036012920x15958782196790","url":null,"abstract":"The purpose of this research was to proce or disprove the widely held beliefs that X-ray radiation used in medical or dental applications may affect the normal oral flora, and may also have effects on the micro-organisms existing in the oral cavity of every person. Such beliefs might\u0000 be due to the common knowledge that radiation therapy has been utilized for the long time in the cancer patients to destroy the cancerous cells, and that radiation are also widely used in agriculture to prolong the shelf life of the farm products by destroying the decay-causing microorganisms\u0000 existing in the agricultural products such as meats, produce, etc. Since very few research has been conducted in this concerned area, and not much pertinent information in the scientific literature could be found in this regard, a research experiment was set up to find out if these common\u0000 beliefs have any merits. The research experiment involved selecting BDORT(By-digital O-Ring Test) positive dishes to incubate human saliva collected from a volunteer patient. Once dish served as control which was not subjected X-ray irradiation. Other dishes were subjected X-ray irradiation\u0000 with various doses of irradiation strength at various time interval such as 1, 3, 5, 7, and 9 seconds to find out if any significant change taken place in the bacteria colonies. The bacterial colonies in the irradiated dishes then compared with that of the control dish so as to draw a conclusion\u0000 if radiation for medical and dental X-ray machines would actually have any bactericidal or/and bacteriostatic effects on the oral micro-organisms flora. Finally, dishes were brought to a local hospital Radiology Department to use high dose of irradaition (used for cancer therapy) on the dishes\u0000 to find out to what extent if such a high dose X-ray irradiation emitted from therapeutic cancer therapy machine would affect the number of bacterial colonies in the dishes as when compared with when dishes were under low doses of X-ray irradiation from dental or medical diagnostic X-ray machines.\u0000 During the experiment, dishes, when irradiated, were sent to a certified medical technologist in the hospital laboratory to count the number of the bacterial colonies, and each change in the bacterial colony number were recorded in to data for biostatic analysis. The data were compared with\u0000 BDORT negativity scores collected each time before and after dishes were irradiated, thus to find out if any corresponding confirmity between traditional laboratory findings and the score changes from BDORT findings. The results of this experiment revealed the fact that radiation from regular\u0000 diagnostic X-ray machines possess little effect on bacteria, and that they have little effect on both cells and bacteria present in the human body. Neverthless, dramatically increasing the high radiation dosages does have potential to both inhibit and destroy bacteria. Therefore,using irradiation\u0000 techniques to inhibit bacterial growth is only useful ","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"45 1","pages":"3-14"},"PeriodicalIF":0.3,"publicationDate":"2020-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47956555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-18DOI: 10.21203/rs.3.rs-57478/v1
Jia Li, Wei Lu, Wei Huang, Song Wu, Yimeng Fu, Jianyi Chen, Zhongsheng Huang, Xiaohong Zhou, Hua Wang
Although acupuncture therapy, as a preventive and therapeutic technique, is low or no risk for some cardiovascular diseases, its role in safeguarding myocardial hypertrophy (MH) has not been clarified. Therefore, this study focused on examining whether electroacupuncture (EA) can alleviate MH by up-regulating tryptophan 5-serotonin (5-HT) metabolism, depressive disorder, and gastrointestinal dysfunction in MH rat models. We evaluated the effect of EA on myocardial ischemia in the ISO-induced myocardial hypertrophy (MH) rat model. Forty Sprague–Dawley (SD) rats aged 3 months were randomly divided into normal group, model group, EA group, and sham EA group with 10 rats in each group. Heart weight/body weight ratio (HW/BW) and left ventricular weight/body weight ratio (LVW/BW) were used to evaluate the regression of MH. Cardiac pathology was evaluated by ultrastructural observation and hematoxylin and eosin (H&E) staining. Sucrose preference test (SPT), forced swimming test (FSW), and open-air test (OFT) were used to study depressive behavior. The gastric retention and intestinal transport were evaluated by carbon powder labeling and the expression of 5-HT in the cerebral cortex and ileum was detected by immunohistochemistry. Depression-like symptoms in rats can be relieved by EA therapy. For instance, the consumption rate of sucrose solution was higher, the total distance and central distance in the open field test were increased, and EA treatment could reduce the immobility time of these rats. Swimming, struggling and immobility latencies were also increased in the forced swimming test. After EA treatment, the expression of 5-HT in the cerebral cortex and ileum increased. Finally, we found that EA ameliorated ischemia, depression, and gastrointestinal dysfunction in ISO-induced MH rats, which may be related to 5-HT metabolism.
{"title":"Electroacupuncture Attenuates Depressive Disorder and Gastrointestinal Dysfunction in Myocardial Hypertrophy via Upregulation of 5-HT on Rats","authors":"Jia Li, Wei Lu, Wei Huang, Song Wu, Yimeng Fu, Jianyi Chen, Zhongsheng Huang, Xiaohong Zhou, Hua Wang","doi":"10.21203/rs.3.rs-57478/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-57478/v1","url":null,"abstract":"\u0000 Although acupuncture therapy, as a preventive and therapeutic technique, is low or no risk for some cardiovascular diseases, its role in safeguarding myocardial hypertrophy (MH) has not been clarified. Therefore, this study focused on examining whether electroacupuncture (EA) can alleviate MH by up-regulating tryptophan 5-serotonin (5-HT) metabolism, depressive disorder, and gastrointestinal dysfunction in MH rat models. We evaluated the effect of EA on myocardial ischemia in the ISO-induced myocardial hypertrophy (MH) rat model. Forty Sprague–Dawley (SD) rats aged 3 months were randomly divided into normal group, model group, EA group, and sham EA group with 10 rats in each group. Heart weight/body weight ratio (HW/BW) and left ventricular weight/body weight ratio (LVW/BW) were used to evaluate the regression of MH. Cardiac pathology was evaluated by ultrastructural observation and hematoxylin and eosin (H&E) staining. Sucrose preference test (SPT), forced swimming test (FSW), and open-air test (OFT) were used to study depressive behavior. The gastric retention and intestinal transport were evaluated by carbon powder labeling and the expression of 5-HT in the cerebral cortex and ileum was detected by immunohistochemistry. Depression-like symptoms in rats can be relieved by EA therapy. For instance, the consumption rate of sucrose solution was higher, the total distance and central distance in the open field test were increased, and EA treatment could reduce the immobility time of these rats. Swimming, struggling and immobility latencies were also increased in the forced swimming test. After EA treatment, the expression of 5-HT in the cerebral cortex and ileum increased. Finally, we found that EA ameliorated ischemia, depression, and gastrointestinal dysfunction in ISO-induced MH rats, which may be related to 5-HT metabolism. \u0000","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42856255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-12DOI: 10.21203/rs.3.rs-52205/v1
Na Shi, Chuqiong Zhang, Long Han, Gang Ouyang
Background: To evaluate the effect of acupoint embedding on intestinal flora in rats with ovariectomized osteoporosis. Methods: Thirty-two 3-month-old female SD rats were randomized into four groups: Group A without operation or treatment, Group B treated with the sham operation, Group C treated with the operation and without any treatment, and Group E treated with operation and acupoint embedding therapy. The osteoporosis animal model was generated by removing bilateral ovaries of rats, and unilateral "Shenshu"(BL23) and "Zusanli" (ST36) of rats were selected for embedding therapy every time. The therapy was performed once every 1 week for 12 consecutive weeks. Results: The bone mineral density of femur and tibia and the estrogen levels in Group E are statistically higher than those in Group C (p<0.05). The Beta diversity analysis shows the difference in the diversity and abundance of flora between Group A and Group C is statistically significant (p<0.01). In Group E, the abundance of probiotics such as firmicutes and bacteroidetes at the phylum level, lactobacillaceae, lachnospiraceae, muribaculaceae, and bacteroidaceae at the family level, lactobacillus gasseri, lactobacillus reuteri, lactobacillus intestinalis, and bacteroides acidifaciens at the species level is higher than those in Group C. The contents of short-chain fatty acid in Group E are significantly higher than those in Group C (p<0.05). Conclusion: Acupoint catgut embedding can improve bone density and increase the estrogen level in rats with osteoporosis, which maybe attributed to regulating composition and abundance of intestinal flora, and increasing the content of short-chain fatty acids.
{"title":"Effect of Acupoint Catgut Embedding on Intestinal Flora in Rats with Ovariectomized Osteoporosis","authors":"Na Shi, Chuqiong Zhang, Long Han, Gang Ouyang","doi":"10.21203/rs.3.rs-52205/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-52205/v1","url":null,"abstract":"\u0000 Background: To evaluate the effect of acupoint embedding on intestinal flora in rats with ovariectomized osteoporosis. Methods: Thirty-two 3-month-old female SD rats were randomized into four groups: Group A without operation or treatment, Group B treated with the sham operation, Group C treated with the operation and without any treatment, and Group E treated with operation and acupoint embedding therapy. The osteoporosis animal model was generated by removing bilateral ovaries of rats, and unilateral \"Shenshu\"(BL23) and \"Zusanli\" (ST36) of rats were selected for embedding therapy every time. The therapy was performed once every 1 week for 12 consecutive weeks. Results: The bone mineral density of femur and tibia and the estrogen levels in Group E are statistically higher than those in Group C (p<0.05). The Beta diversity analysis shows the difference in the diversity and abundance of flora between Group A and Group C is statistically significant (p<0.01). In Group E, the abundance of probiotics such as firmicutes and bacteroidetes at the phylum level, lactobacillaceae, lachnospiraceae, muribaculaceae, and bacteroidaceae at the family level, lactobacillus gasseri, lactobacillus reuteri, lactobacillus intestinalis, and bacteroides acidifaciens at the species level is higher than those in Group C. The contents of short-chain fatty acid in Group E are significantly higher than those in Group C (p<0.05). Conclusion: Acupoint catgut embedding can improve bone density and increase the estrogen level in rats with osteoporosis, which maybe attributed to regulating composition and abundance of intestinal flora, and increasing the content of short-chain fatty acids.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42812119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-21DOI: 10.3727/036012920x15779969212982
Y. Omura
While a visiting Professor at the University of Paris, VI (formerly Sorvonne) more than 40 years ago, the Author became very good friends with Dr. Paul Nogier who periodically gave seminars and workshops in Paris. After the author diagnosed his cervical problem & offered him simple help, Dr. Nogier asked the Author to present lectures and demonstrations on the effects of ear stimulation, namely the effects of acupuncture & electrical stimulation of the ear lobules. It is only now, in 2019 that we have discovered 2–5 minute high frequency stimulation of the ear lobule inhibits cancer activity for 1– 4 hours post stimulation. Although the procedure is extremely simple. First take optimal dose of Vitamin D3, which has the most essential 10 unique beneficial factors required for every human cell activity. Next, apply high frequency stimulation to ear lobule while the worst ear lobule is held by all fingers with vibrator directly touching the surface of the worst ear lobule, preferably after patient repeatedly takes optimal dose of Vitamin D3. When the worst ear lobule is held between thumb & index fingers and applying mechanical stimulation of 250 ~ 500 mechanical vibration/second for 2 ~ 5 minutes using an electrical vibrator, there is rapid disappearance of cancer activity in both the brain and rest of the body for short time duration 1 ~ 4 hours. The effect often increases by additional pressure by holding fingers. As of May 2019, the Author found that many people from various regions of the world developed early stages of multiple cancers. For evaluation of this study, U. S. patented Bi-Digital O-Ring Test (BDORT) was used which was developed by the Author while doing his Graduate experimental physics research at Colombia University. BDORT was found to be most essential for determining the beneficial effects as well as harmful effects of any substance or treatment. Using BDORT, Author was the first to recognize severe increasing mid-backache was an early sign of pancreatic cancer of President of New York State Board of Medicine after top pain specialists failed to detect the cause after 3 years of effort, while the BDORT showed early stages of cancer whereas conventional X-Ray of the pancreas did not show any cancer image until 2 months after Author detected with BDORT. For example, the optimal dose of the banana is usually about 2.0 - 2.5 millimeters cross section of the banana. A whole banana is more than 50 ~ 100 times the optimal dose. Any substance eaten in more than 25 times of its optimal dose becomes highly toxic and creates DNA mutations which can cause multiple malignancies in the presence of strong electro-magnetic field. With standard medication given by doctor, patients often become sick and they are unable to reduce body weight, unless medication is reduced or completely stopped. When the amount of zinc is very high, DNA often becomes unstable and multiple cancers can grow rapidly in the presence of strong electromagnetic
{"title":"High Frequency Vibration of Worst Ear Lobule induced short period of Rapid Inhibition of any Cancer Activity including Advanced Terminal Cancers, Most Malignant Brain Tumor \"Glioblastoma\" as well as Cancer of Lung, Breast & Gastrointestinal Cancers particularly Pancreatic Cancer.","authors":"Y. Omura","doi":"10.3727/036012920x15779969212982","DOIUrl":"https://doi.org/10.3727/036012920x15779969212982","url":null,"abstract":"While a visiting Professor at the University of Paris, VI (formerly Sorvonne) more than 40 years ago, the Author became very good friends with Dr. Paul Nogier who periodically gave seminars and workshops in Paris. After the author diagnosed his cervical problem & offered him simple\u0000 help, Dr. Nogier asked the Author to present lectures and demonstrations on the effects of ear stimulation, namely the effects of acupuncture & electrical stimulation of the ear lobules. It is only now, in 2019 that we have discovered 2–5 minute high frequency stimulation of the\u0000 ear lobule inhibits cancer activity for 1– 4 hours post stimulation. Although the procedure is extremely simple. First take optimal dose of Vitamin D3, which has the most essential 10 unique beneficial factors required for every human cell activity. Next, apply high frequency stimulation\u0000 to ear lobule while the worst ear lobule is held by all fingers with vibrator directly touching the surface of the worst ear lobule, preferably after patient repeatedly takes optimal dose of Vitamin D3. When the worst ear lobule is held between thumb & index fingers and applying mechanical\u0000 stimulation of 250 ~ 500 mechanical vibration/second for 2 ~ 5 minutes using an electrical vibrator, there is rapid disappearance of cancer activity in both the brain and rest of the body for short time duration 1 ~ 4 hours. The effect often increases by additional pressure by holding fingers.\u0000 As of May 2019, the Author found that many people from various regions of the world developed early stages of multiple cancers. For evaluation of this study, U. S. patented Bi-Digital O-Ring Test (BDORT) was used which was developed by the Author while doing his Graduate experimental physics\u0000 research at Colombia University. BDORT was found to be most essential for determining the beneficial effects as well as harmful effects of any substance or treatment. Using BDORT, Author was the first to recognize severe increasing mid-backache was an early sign of pancreatic cancer of President\u0000 of New York State Board of Medicine after top pain specialists failed to detect the cause after 3 years of effort, while the BDORT showed early stages of cancer whereas conventional X-Ray of the pancreas did not show any cancer image until 2 months after Author detected with BDORT. For example,\u0000 the optimal dose of the banana is usually about 2.0 - 2.5 millimeters cross section of the banana. A whole banana is more than 50 ~ 100 times the optimal dose. Any substance eaten in more than 25 times of its optimal dose becomes highly toxic and creates DNA mutations which can cause multiple\u0000 malignancies in the presence of strong electro-magnetic field. With standard medication given by doctor, patients often become sick and they are unable to reduce body weight, unless medication is reduced or completely stopped. When the amount of zinc is very high, DNA often becomes unstable\u0000 and multiple cancers can grow rapidly in the presence of strong electromagnetic ","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"44 1","pages":"241-249"},"PeriodicalIF":0.3,"publicationDate":"2020-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45046420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-21DOI: 10.3727/036012920x15779969212928
Momir Dunjic, S. Turini, D. Krstić, Katarina Dunjić, M. Dunjic, Bojan Rajevic, Petar Miodragović
Radiofrequency therapy is an unconventional method, already applied for some time, with numerous results in numerous clinical pictures. Our group has developed a software, later called SONGENPROT-SOLARIS, capable of directly converting nucleotide sequences (DNA and/or RNA) and amino acid sequences (polypeptides and proteins) into musical sequences, based on mathematic matrices, designed by the French physicist and musician Joel Sternheimer, which allows to associate a musical note with a nucleotide or an amino acid. Innovation in our software is that, in the algorithm that defines it, a variant is directly implemented that allows the reproduction of sounds, phase-shifted by 30 Hz, between one ear and another reproducing the phenomenon of Binaural Tones, capable of induce a specific brain activity and also the release of particles called solitons. Thanks to this software we have developed a technique called MMT (Molecular Music Therapy) and currently, we are in the phase of applying the technique on a cohort of 91 patients, with a high spectrum of clinical pictures, examining the same, using the technique Bi-Digital-ORing-Test (BDORT), before and after treatment with MMT. Aim of project is to stimulate the expression of a specific gene (the same genetic sequence that the patient listens to, translated into music), only through the use of sound sequences. We have concentrated our attention on three main molecules: Sirtuin-1, Telomers and TP-53. The results obtained with BDORT, after treatment with MMT, showed a significant increase in the values of the three molecules, on all the examined patients, demonstrating the operative efficacy of the technique and the its applicability to numerous diseases. In order to confirm the data obtained by BDORT, we propose, with the help of an accredited laboratory, to perform epigenetic tests on the three parameters listed above, paving the way to understanding how frequencies can influence gene expression.
{"title":"Application of the Innovative and Non-Invasive Technique, Molecular Music Therapy (MMT), Bio-Frequency Therapy, for the Treatment of a Wide Range of Disorders and Pathologies, with Consequent Verification of Molecular Parameters by Using Bi-Digital O-Ring Test (BDORT)","authors":"Momir Dunjic, S. Turini, D. Krstić, Katarina Dunjić, M. Dunjic, Bojan Rajevic, Petar Miodragović","doi":"10.3727/036012920x15779969212928","DOIUrl":"https://doi.org/10.3727/036012920x15779969212928","url":null,"abstract":"Radiofrequency therapy is an unconventional method, already applied for some time, with numerous results in numerous clinical pictures. Our group has developed a software, later called SONGENPROT-SOLARIS, capable of directly converting nucleotide sequences (DNA and/or RNA) and amino\u0000 acid sequences (polypeptides and proteins) into musical sequences, based on mathematic matrices, designed by the French physicist and musician Joel Sternheimer, which allows to associate a musical note with a nucleotide or an amino acid. Innovation in our software is that, in the algorithm\u0000 that defines it, a variant is directly implemented that allows the reproduction of sounds, phase-shifted by 30 Hz, between one ear and another reproducing the phenomenon of Binaural Tones, capable of induce a specific brain activity and also the release of particles called solitons. Thanks\u0000 to this software we have developed a technique called MMT (Molecular Music Therapy) and currently, we are in the phase of applying the technique on a cohort of 91 patients, with a high spectrum of clinical pictures, examining the same, using the technique Bi-Digital-ORing-Test (BDORT), before\u0000 and after treatment with MMT. Aim of project is to stimulate the expression of a specific gene (the same genetic sequence that the patient listens to, translated into music), only through the use of sound sequences. We have concentrated our attention on three main molecules: Sirtuin-1, Telomers\u0000 and TP-53. The results obtained with BDORT, after treatment with MMT, showed a significant increase in the values of the three molecules, on all the examined patients, demonstrating the operative efficacy of the technique and the its applicability to numerous diseases. In order to confirm\u0000 the data obtained by BDORT, we propose, with the help of an accredited laboratory, to perform epigenetic tests on the three parameters listed above, paving the way to understanding how frequencies can influence gene expression.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"44 1","pages":"177-189"},"PeriodicalIF":0.3,"publicationDate":"2020-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43101954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-21DOI: 10.3727/036012920x15779969212955
B. Ebrahimi, F. Forouzanfar, H. Azizi, Hoda Khoshdel-sarkarizi, H. Sadeghnia, A. Rajabzadeh
Diabetes mellitus is a metabolic disorder with increasing global prevalence. It is characterized by impaired glucose utilization that leads to chronic hyperglycemia which is a result of the body's inability to produce insulin (diabetes type I) or inability to make use of insulin (diabetes type II). Long-term hyperglycemia can cause damage to multiple systems, and microvascular and macrovascular complications lead to myocardial infarction, blindness, stroke and renal failure. Diabetes affected 382 million people globally in 2013, and it is estimated to rise up to 592 million by 2035. In spite of its management, both microvascular and macrovascular complications partly linked to oxidative stress are not efficiently prevented. Glibenclamide was approved on the U. S. market for treatment of diabetes type II in 1984. ATP-sensitive potassium channels (KATP) are widely distributed and present in a number of tissues including muscle, pancreatic beta cells and the brain. Glibenclamide closed KATP channels, which leads to depolarization of the cells and insulin secretion. Acupuncture is also a very significant therapeutic method in the complementary medicine. ST36 (Zusanli), CV4 (Guanyuan) and CV12 (Zhongwan) are several acupoints that have been used for treatment of diabetes. In this study for evaluating the effects of glibenclamide and electroacupuncture, 3 parameters such as malondialdehyde, ferric reducing antioxidant power and thiol will be measured. Malondialdehyde (MDA) is the organic compound and it is a marker for oxidative stress. Antioxidants are compounds that inhibit oxidation. Oxidation is a chemical reaction that can produce free radicals, thereby leading to chain reactions that may damage the cells of organisms. Antioxidants such as FRAP and thiol are useful parameters of assessment of oxidative stress. The aim of this study was to evaluate the effects of Electroacupuncture (EA) plus glibenclamide (G) as a novel therapy on diabetic rats and maybe for human. Fifty-four male Wistar rats were randomly divided to 9 groups: 1 non-diabetic control group and 8 diabetic groups (1 sham control group and 7 experimental groups; D/G 2.5 mg/kg, D/G 5 mg/kg, D/G 10 mg/kg, EA, D/EA/G 2.5 mg/kg, D/EA/G 5 mg/kg, and D/EA/G 10 mg/kg). Diabetes was induced by intraperitoneal injection of streptozotocin with high-fat diet. At the end of course, blood samples were obtained. Combination therapy of EA and glibenclamide 5 mg/kg decreased blood glucose better than single therapies (p<0.05) and showed 41 percent decrease in blood glucose as compared to D/G 5 mg/kg group. Combination of EA and glibenclamide 10 mg/kg showed the best effect for decreasing the malondialdehyde level (p<0.05) and also showed 43 percent decrease in comparison to D/G 10 mg/kg group. Combination of glibenclamide 2.5 mg/kg and EA increased the FRAP level better than other treatment groups (P<0.001) andachieved the ferric reducing antioxidant power level near to normal range. Combina
{"title":"Effect of Electroacupuncture and Glibenclamide on Blood Glucose Level and Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats and Possible Human Implications","authors":"B. Ebrahimi, F. Forouzanfar, H. Azizi, Hoda Khoshdel-sarkarizi, H. Sadeghnia, A. Rajabzadeh","doi":"10.3727/036012920x15779969212955","DOIUrl":"https://doi.org/10.3727/036012920x15779969212955","url":null,"abstract":"Diabetes mellitus is a metabolic disorder with increasing global prevalence. It is characterized by impaired glucose utilization that leads to chronic hyperglycemia which is a result of the body's inability to produce insulin (diabetes type I) or inability to make use of insulin (diabetes\u0000 type II). Long-term hyperglycemia can cause damage to multiple systems, and microvascular and macrovascular complications lead to myocardial infarction, blindness, stroke and renal failure. Diabetes affected 382 million people globally in 2013, and it is estimated to rise up to 592 million\u0000 by 2035. In spite of its management, both microvascular and macrovascular complications partly linked to oxidative stress are not efficiently prevented. Glibenclamide was approved on the U. S. market for treatment of diabetes type II in 1984. ATP-sensitive potassium channels (KATP) are widely\u0000 distributed and present in a number of tissues including muscle, pancreatic beta cells and the brain. Glibenclamide closed KATP channels, which leads to depolarization of the cells and insulin secretion. Acupuncture is also a very significant therapeutic method in the complementary medicine.\u0000 ST36 (Zusanli), CV4 (Guanyuan) and CV12 (Zhongwan) are several acupoints that have been used for treatment of diabetes. In this study for evaluating the effects of glibenclamide and electroacupuncture, 3 parameters such as malondialdehyde, ferric reducing antioxidant power and thiol will be\u0000 measured. Malondialdehyde (MDA) is the organic compound and it is a marker for oxidative stress. Antioxidants are compounds that inhibit oxidation. Oxidation is a chemical reaction that can produce free radicals, thereby leading to chain reactions that may damage the cells of organisms. Antioxidants\u0000 such as FRAP and thiol are useful parameters of assessment of oxidative stress. The aim of this study was to evaluate the effects of Electroacupuncture (EA) plus glibenclamide (G) as a novel therapy on diabetic rats and maybe for human. Fifty-four male Wistar rats were randomly divided to\u0000 9 groups: 1 non-diabetic control group and 8 diabetic groups (1 sham control group and 7 experimental groups; D/G 2.5 mg/kg, D/G 5 mg/kg, D/G 10 mg/kg, EA, D/EA/G 2.5 mg/kg, D/EA/G 5 mg/kg, and D/EA/G 10 mg/kg). Diabetes was induced by intraperitoneal injection of streptozotocin with high-fat\u0000 diet. At the end of course, blood samples were obtained. Combination therapy of EA and glibenclamide 5 mg/kg decreased blood glucose better than single therapies (p<0.05) and showed 41 percent decrease in blood glucose as compared to D/G 5 mg/kg group. Combination of EA and glibenclamide\u0000 10 mg/kg showed the best effect for decreasing the malondialdehyde level (p<0.05) and also showed 43 percent decrease in comparison to D/G 10 mg/kg group. Combination of glibenclamide 2.5 mg/kg and EA increased the FRAP level better than other treatment groups (P<0.001) andachieved the\u0000 ferric reducing antioxidant power level near to normal range. Combina","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"44 1","pages":"213-228"},"PeriodicalIF":0.3,"publicationDate":"2020-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42541912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-21DOI: 10.3727/036012920x15779969212946
Hao Xu, Yingying Fan, Xiangzhong Meng, J. Luan, Hua Guan, Xianlin Su, Lixian Xu, D. Lu
The stress response can be triggered during the perioperative period by tension, fear, anesthesia, surgical trauma, and various postoperative adverse stimuli, leading to significant changes in the endocrine, metabolic, and immune systems of patients. In particular, immunosuppression induced by the stress response has adverse effects on the postoperative recovery of patients. As acupuncture stimulation-related technologies have been rapidly developed and applied in recent years, a number of basic and clinical studies have confirmed that acupoint stimulation can regulate the immune system via local, neurological, and endocrine pathways. Moreover, acupoint stimulation treatment has also been shown to reduce the adverse effects of immunosuppression by affecting the release of various cytokines, such as interleukin (IL) 1, IL-2, IL-4, IL-6, IL-10, IFN-γ, and tumor necrosis factor (TNF) β, immunoglobulins, complement proteins, and T cell markers such as CD3, CD4, and CD4/CD8 via the regulation of macrophages, neutrophils, NK cells, and endogenous opioids. In addition, acupuncture stimulation treatment during the perioperative period can also significantly decrease the amount of anesthetic required for anesthesia, effectively reduce nausea and vomiting, relieve post-operative pain, and accelerate the recovery of physiological functions. Therefore, acupuncture stimulation treatment has shown important potential for clinical applications.
{"title":"Use of Acupoint Stimulation to Regulate Perioperative Stress-Induced Immunosuppression","authors":"Hao Xu, Yingying Fan, Xiangzhong Meng, J. Luan, Hua Guan, Xianlin Su, Lixian Xu, D. Lu","doi":"10.3727/036012920x15779969212946","DOIUrl":"https://doi.org/10.3727/036012920x15779969212946","url":null,"abstract":"The stress response can be triggered during the perioperative period by tension, fear, anesthesia, surgical trauma, and various postoperative adverse stimuli, leading to significant changes in the endocrine, metabolic, and immune systems of patients. In particular, immunosuppression\u0000 induced by the stress response has adverse effects on the postoperative recovery of patients. As acupuncture stimulation-related technologies have been rapidly developed and applied in recent years, a number of basic and clinical studies have confirmed that acupoint stimulation can regulate\u0000 the immune system via local, neurological, and endocrine pathways. Moreover, acupoint stimulation treatment has also been shown to reduce the adverse effects of immunosuppression by affecting the release of various cytokines, such as interleukin (IL) 1, IL-2, IL-4, IL-6, IL-10, IFN-γ,\u0000 and tumor necrosis factor (TNF) β, immunoglobulins, complement proteins, and T cell markers such as CD3, CD4, and CD4/CD8 via the regulation of macrophages, neutrophils, NK cells, and endogenous opioids. In addition, acupuncture stimulation treatment during the perioperative period can\u0000 also significantly decrease the amount of anesthetic required for anesthesia, effectively reduce nausea and vomiting, relieve post-operative pain, and accelerate the recovery of physiological functions. Therefore, acupuncture stimulation treatment has shown important potential for clinical\u0000 applications.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"44 1","pages":"203-212"},"PeriodicalIF":0.3,"publicationDate":"2020-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44731833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-21DOI: 10.3727/036012920x15779969212973
Yasemin Gündüztepe, S. Mit, Ersel Geçioğlu, Neslihan Gürbüz, S. Neşelioğlu, O. Erel, C. Çevik
The aim of this study was to investigate the effect of acupuncture on dynamic thiol–disulphide homeostasis and ischemia-modified albumin (IMA) levels as a novel oxidative stress parameter in migraine patients. Acupuncture treatment was applied to the 22 volunteer migraine patients. The acupuncture treatment consists of 5 sessions with 2 sessions per week. Blood samples have been collected before performing acupuncture, after the 1st session and after the 5th session of the acupuncture. And for the control group blood samples were collected only once. A total of 44 participants were included in the study, and 24 of whom were diagnosed with migraine. The total thiol, native thiol and ischemia-modified albumin (IMA) levels in the serum were measured in all patients and healthy individuals. The dynamic disulphide bond and ischemia-modified albumin (IMA) levels were calculated from these values. The total and native thiol levels of migraine patients participating in the study were found to be significantly higher than the total and native thiol levels of healthy individuals. The total thiol, native thiol, and dynamic disulphide bond levels, and ischemia-modified albumin (IMA) were correlated with attack frequency, pain intensity, or migraine type. Thiol-disülfide homeostasis can play roles in the etiology and severity of Migraine.
{"title":"The Impact of Acupuncture Treatment on dynamic thiol–disulphide homeostasis and ischemia-modified albumin levels to assess the oxidative stress in Migraine Patients","authors":"Yasemin Gündüztepe, S. Mit, Ersel Geçioğlu, Neslihan Gürbüz, S. Neşelioğlu, O. Erel, C. Çevik","doi":"10.3727/036012920x15779969212973","DOIUrl":"https://doi.org/10.3727/036012920x15779969212973","url":null,"abstract":"The aim of this study was to investigate the effect of acupuncture on dynamic thiol–disulphide homeostasis and ischemia-modified albumin (IMA) levels as a novel oxidative stress parameter in migraine patients. Acupuncture treatment was applied to the 22 volunteer migraine patients.\u0000 The acupuncture treatment consists of 5 sessions with 2 sessions per week. Blood samples have been collected before performing acupuncture, after the 1st session and after the 5th session of the acupuncture. And for the control group blood samples were collected only once. A total of 44 participants\u0000 were included in the study, and 24 of whom were diagnosed with migraine. The total thiol, native thiol and ischemia-modified albumin (IMA) levels in the serum were measured in all patients and healthy individuals. The dynamic disulphide bond and ischemia-modified albumin (IMA) levels were\u0000 calculated from these values. The total and native thiol levels of migraine patients participating in the study were found to be significantly higher than the total and native thiol levels of healthy individuals. The total thiol, native thiol, and dynamic disulphide bond levels, and ischemia-modified\u0000 albumin (IMA) were correlated with attack frequency, pain intensity, or migraine type. Thiol-disülfide homeostasis can play roles in the etiology and severity of Migraine.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"44 1","pages":"229-240"},"PeriodicalIF":0.3,"publicationDate":"2020-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44315337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-21DOI: 10.3727/036012920x15779969212937
Jiarun Zhang, Weizhen Wu, Li Wan, Yun-xian Zhang, Ruoqi Li, Ning Li, D. Guo
Gastric ulcer is a common digestive system disease, which leads to the serious decline in the life quality and effective therapies are in urgent need. Midnightmidday ebb flow acupunctures including Najia and Nazi acupunctures have been applied in clinics in China for more than one thousand years and the acupoints are selected according to the time rhythm. Najia acupuncture, namely day-prescription of acupoints, divides ten days (one cycle) into 120 two-hour periods and each period is coordinated with a meridian. Meanwhile, Nazi acupuncture, namely hour-prescription of acupoints, divides a day(one cycle) into 12 two-hour periods and each period is coordinated with a meridian. When there is a disease in a certain meridian, the relevant acupoints are operated in the corresponding time. However, the comparative efficacies of Najia and Nazi acupunctures on promoting the healing of gastric ulcer are still unknown. In this study, gastric ulcer rats were induced by acetic acid and were randomly divided into four groups: non-acupuncture group, normal acupuncture group, Najia acupuncture group and Nazi acupuncture group. After different treatments for continuous 10 days, the gained weight, gastric ulcer area and the thickness of gastric mucosa were measured and analyzed. Decreased ulcer area, increased mucosa thickness and dense scar tissue occurred in the Najia group and Nazi group compared to that in the non-acupuncture group (P<0.05). However, there were no significant differences between Najia and Nazi acupuncture groups (P>0.05). Thickness of gastric mucosa in Nazi acupuncture group increased significantly compared to normal acupuncture group (P<0.05). Therefore, the findings in this study indicated that Najia and Nazi acupunctures had gastroprotective effects and accelerated the healing of acetic acid-induced gastric ulcer in rats, but no significant differences between them. It may provide valuable information for development of acupuncture for the treatment of gastric ulcer in clinics.
{"title":"Comparative Study of Najia and Nazi Acupunctures to Reduce Rat Gastric Ulcer","authors":"Jiarun Zhang, Weizhen Wu, Li Wan, Yun-xian Zhang, Ruoqi Li, Ning Li, D. Guo","doi":"10.3727/036012920x15779969212937","DOIUrl":"https://doi.org/10.3727/036012920x15779969212937","url":null,"abstract":"Gastric ulcer is a common digestive system disease, which leads to the serious decline in the life quality and effective therapies are in urgent need. Midnightmidday ebb flow acupunctures including Najia and Nazi acupunctures have been applied in clinics in China for more than one thousand\u0000 years and the acupoints are selected according to the time rhythm. Najia acupuncture, namely day-prescription of acupoints, divides ten days (one cycle) into 120 two-hour periods and each period is coordinated with a meridian. Meanwhile, Nazi acupuncture, namely hour-prescription of acupoints,\u0000 divides a day(one cycle) into 12 two-hour periods and each period is coordinated with a meridian. When there is a disease in a certain meridian, the relevant acupoints are operated in the corresponding time. However, the comparative efficacies of Najia and Nazi acupunctures on promoting the\u0000 healing of gastric ulcer are still unknown. In this study, gastric ulcer rats were induced by acetic acid and were randomly divided into four groups: non-acupuncture group, normal acupuncture group, Najia acupuncture group and Nazi acupuncture group. After different treatments for continuous\u0000 10 days, the gained weight, gastric ulcer area and the thickness of gastric mucosa were measured and analyzed. Decreased ulcer area, increased mucosa thickness and dense scar tissue occurred in the Najia group and Nazi group compared to that in the non-acupuncture group (P<0.05). However,\u0000 there were no significant differences between Najia and Nazi acupuncture groups (P>0.05). Thickness of gastric mucosa in Nazi acupuncture group increased significantly compared to normal acupuncture group (P<0.05). Therefore, the findings in this study indicated that Najia and Nazi acupunctures\u0000 had gastroprotective effects and accelerated the healing of acetic acid-induced gastric ulcer in rats, but no significant differences between them. It may provide valuable information for development of acupuncture for the treatment of gastric ulcer in clinics.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"44 1","pages":"191-201"},"PeriodicalIF":0.3,"publicationDate":"2020-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46449888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：