Pub Date : 2018-04-06DOI: 10.4467/16890027AP.18.001.8395
O. Anderson
A Half-century of Research on Free-living Amoebae (1965-2017): Review of Biogeographic, Ecological and Physiological Studies
自由生活变形虫研究的半个世纪(1965-2017):生物地理学、生态学和生理学研究综述
{"title":"A half-century of research on free-living amoebae (1965–2017): review of biogeographic, ecological and physiological studies","authors":"O. Anderson","doi":"10.4467/16890027AP.18.001.8395","DOIUrl":"https://doi.org/10.4467/16890027AP.18.001.8395","url":null,"abstract":"A Half-century of Research on Free-living Amoebae (1965-2017): Review of Biogeographic, Ecological and Physiological Studies","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"57 1","pages":"1-28"},"PeriodicalIF":0.5,"publicationDate":"2018-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48418079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-06DOI: 10.4467/16890027ap.18.003.8397
Piotr Solarczyk, A. Wojtkowiak-Giera, M. Hołysz, Anna Słodkowicz-Kowalska, P. Jagodziński, K. Stojecki, A. Rocka, A. Majewska, Łukasz Skrzypczak
New primers for fast detection of Giardia duodenalis assemblages A and B using realtime PCR
实时PCR快速检测十二指肠贾第鞭毛虫组合A和B的新引物
{"title":"New primers for fast detection of Giardia duodenalis assemblages A and B using realtime PCR","authors":"Piotr Solarczyk, A. Wojtkowiak-Giera, M. Hołysz, Anna Słodkowicz-Kowalska, P. Jagodziński, K. Stojecki, A. Rocka, A. Majewska, Łukasz Skrzypczak","doi":"10.4467/16890027ap.18.003.8397","DOIUrl":"https://doi.org/10.4467/16890027ap.18.003.8397","url":null,"abstract":"New primers for fast detection of Giardia duodenalis assemblages A and B using realtime PCR","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"2018 1","pages":"43-48"},"PeriodicalIF":0.5,"publicationDate":"2018-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48493630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-06DOI: 10.4467/16890027AP.18.006.8400
Franciane Cedrola, P. Fregulia, M. D’Agosto, R. Dias
In our study, we aimed to identify and quantify the intestinal ciliates in a Brazilian capybara and to compare the obtained data with previous studies on the capybara ciliate community in other geographic locations within the American continent. We identified 20 species belonging to four families and ten genera. This is the first study on intestinal ciliates in Brazilian capybara since the last reports for the country in the 1960s. Among the identified species, Anacharon gracilis, A. lepturus, Cycloposthium bursa, Monoposthium cynodontum, Ogimotopsis pumila, Paracunhamunizia calocoma, Protohallia nana and Uropogon urai were recorded for the first time in Brazil, and the giant ciliate Muniziella cunhai was observed for the second time in a symbiotic association with capybaras in the country. The present study highlighted the importance of knowing the gastrointestinal ciliate community associated with wild hosts in order to better understand their geographic distribution and host specificity.
{"title":"Intestinal ciliates of Brazilian Capybara (Hydrochoerus hydrochaeris L.)","authors":"Franciane Cedrola, P. Fregulia, M. D’Agosto, R. Dias","doi":"10.4467/16890027AP.18.006.8400","DOIUrl":"https://doi.org/10.4467/16890027AP.18.006.8400","url":null,"abstract":"In our study, we aimed to identify and quantify the intestinal ciliates in a Brazilian capybara and to compare the obtained data with previous studies on the capybara ciliate community in other geographic locations within the American continent. We identified 20 species belonging to four families and ten genera. This is the first study on intestinal ciliates in Brazilian capybara since the last reports for the country in the 1960s. Among the identified species, Anacharon gracilis, A. lepturus, Cycloposthium bursa, Monoposthium cynodontum, Ogimotopsis pumila, Paracunhamunizia calocoma, Protohallia nana and Uropogon urai were recorded for the first time in Brazil, and the giant ciliate Muniziella cunhai was observed for the second time in a symbiotic association with capybaras in the country. The present study highlighted the importance of knowing the gastrointestinal ciliate community associated with wild hosts in order to better understand their geographic distribution and host specificity.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"2018 1","pages":"61-67"},"PeriodicalIF":0.5,"publicationDate":"2018-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48578102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"High Genetic Diversity of Amoebae Belonging to the Genus Mayorella (Amoebozoa, Discosea, Dermamoebida) in Natural Habitats","authors":"A. Glotova, N. Bondarenko, A. Smirnov","doi":"10.4467/16890027ap.18.002.8396","DOIUrl":"https://doi.org/10.4467/16890027ap.18.002.8396","url":null,"abstract":"High Genetic Diversity of Amoebae Belonging to the Genus Mayorella (Amoebozoa, Discosea, Dermamoebida) in Natural Habitats","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"2018 1","pages":"29-42"},"PeriodicalIF":0.5,"publicationDate":"2018-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49473256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4467/16890027ap.18.004.8398
I. Grushevaya, A. Ignatieva, S. Malysh, I. Senderskiy, I. V. Zubarev, A. Kononchuk
{"title":"Spore dimorphism in Nosema pyrausta (Microsporidia, Nosematidae): from morphological evidence to molecular genetic verification","authors":"I. Grushevaya, A. Ignatieva, S. Malysh, I. Senderskiy, I. V. Zubarev, A. Kononchuk","doi":"10.4467/16890027ap.18.004.8398","DOIUrl":"https://doi.org/10.4467/16890027ap.18.004.8398","url":null,"abstract":"","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"57 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70977872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4467/16890027ap.18.017.10093
H. Pérez-Juárez, A. Serrano-Vázquez, E. Lara, C. Ximénez, H. Godínez-Alvarez, S. Rodríguez-Zaragoza, M. M. H. Moreno, L. Eguiarte, Leonardo D. Fernández, L. Rojas-Velázquez, P. Morán, M. Castillo, V. Aguilar, Csic Madrid España Real Jardín Botánico
Population dynamics of amoeboid protists in a tropical desert: seasonal changes and effects of vegetation and soil conditions
热带沙漠中变形虫原生生物的种群动态:季节变化及植被和土壤条件的影响
{"title":"Population dynamics of amoeboid protists in a tropical desert: seasonal changes and effects of vegetation and soil conditions","authors":"H. Pérez-Juárez, A. Serrano-Vázquez, E. Lara, C. Ximénez, H. Godínez-Alvarez, S. Rodríguez-Zaragoza, M. M. H. Moreno, L. Eguiarte, Leonardo D. Fernández, L. Rojas-Velázquez, P. Morán, M. Castillo, V. Aguilar, Csic Madrid España Real Jardín Botánico","doi":"10.4467/16890027ap.18.017.10093","DOIUrl":"https://doi.org/10.4467/16890027ap.18.017.10093","url":null,"abstract":"Population dynamics of amoeboid protists in a tropical desert: seasonal changes and effects of vegetation and soil conditions","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"2018 1","pages":"231-242"},"PeriodicalIF":0.5,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70978362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-14DOI: 10.4467/16890027ap.17.023.7825
Ľ. Rajter, P. Vďačný
We investigated interrelationships between four free-living litostomatean lineages, using 18S rRNA gene and ITS region sequences as well as the secondary structure of the ITS2 molecules. Our phylogenetic analyses confirmed the deep split of free-living litostomateans into Rhynchostomatia and Haptoria represented here by Haptorida, Pleurostomatida, and Spathidiida. This bifurcation is also corroborated by the signature of the rhynchostomatian and haptorian ITS2 molecules. Specifically, the consensus stems of helices II and III are longer by one base pair in Rhynchostomatia, while the terminal loops of both helices are longer by one or two nucleotide/-s in Haptoria. A close relationship of Pleurostomatida and Haptorida is favored by quartet likelihood-mapping and supported by a 5’-AG vs. CU-3’ motif in the variable part of helix II and by two morphological apomorphies, i.e., meridionally extending somatic kineties and a non-three-rowed dorsal brush. Although monophyletic origin of Spathidiida is poorly supported in phylogenetic trees, the unique motif 5’-GA vs. UC-3’ present in the consensus helix II stem could be an important molecular synapomorphy of spathidiids, apart from the ancestrally anteriorly curved somatic kineties and the three-rowed dorsal brush. The peculiar family Pseudoholophryidae has very likely found its phylogenetic home among spathidiids, as an early branching lineage.
{"title":"Constraints on phylogenetic interrelationships among four free-living Litostomatean Lineages inferred from 18S rRNA gene-ITS region sequences and secondary structure of the ITS2 molecule","authors":"Ľ. Rajter, P. Vďačný","doi":"10.4467/16890027ap.17.023.7825","DOIUrl":"https://doi.org/10.4467/16890027ap.17.023.7825","url":null,"abstract":"We investigated interrelationships between four free-living litostomatean lineages, using 18S rRNA gene and ITS region sequences as well as the secondary structure of the ITS2 molecules. Our phylogenetic analyses confirmed the deep split of free-living litostomateans into Rhynchostomatia and Haptoria represented here by Haptorida, Pleurostomatida, and Spathidiida. This bifurcation is also corroborated by the signature of the rhynchostomatian and haptorian ITS2 molecules. Specifically, the consensus stems of helices II and III are longer by one base pair in Rhynchostomatia, while the terminal loops of both helices are longer by one or two nucleotide/-s in Haptoria. A close relationship of Pleurostomatida and Haptorida is favored by quartet likelihood-mapping and supported by a 5’-AG vs. CU-3’ motif in the variable part of helix II and by two morphological apomorphies, i.e., meridionally extending somatic kineties and a non-three-rowed dorsal brush. Although monophyletic origin of Spathidiida is poorly supported in phylogenetic trees, the unique motif 5’-GA vs. UC-3’ present in the consensus helix II stem could be an important molecular synapomorphy of spathidiids, apart from the ancestrally anteriorly curved somatic kineties and the three-rowed dorsal brush. The peculiar family Pseudoholophryidae has very likely found its phylogenetic home among spathidiids, as an early branching lineage.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"2017 1","pages":"255-281"},"PeriodicalIF":0.5,"publicationDate":"2017-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47045423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-14DOI: 10.4467/16890027AP.17.027.7829
T. Tanveer, A. Hameed, Abdul-Aziz A. Bin-Dukhyil, Mohammed A Alaidarous, A. Matin
Naegleria is well recognized for primary amoebic meningoencephalitis which always results into death. To date there is not a single report demonstrating molecular identification of Naegleria from water resources of Pakistan thus the aim of the proposed study. Here, in total 135 various water samples (like domestic tap water, municipal water, sea water, well water, tube well water, canal water, boring water, sewage water, lake water and stream water) were collected across Pakistan and evaluated for Naegleria species. Naegleria australiensis (pathogenic) and Naegleria lovaniensis (non pathogenic) were isolated on non nutrient agar plates and were further identified by PCR and sequencing. To best of our knowledge we have described for the first time the isolation and molecular identification of thermotolerant pathogenic and non pathogenic Naegleria species from diverse water samples including drinking water across Pakistan. The presence of pathogenic Naegleria species in diverse water samples may add the health threat to the community.
{"title":"Isolation of Thermotolerant Pathogenic Naegleria australiensis from Diverse Water Resources Including Household Drinking Water in Pakistan","authors":"T. Tanveer, A. Hameed, Abdul-Aziz A. Bin-Dukhyil, Mohammed A Alaidarous, A. Matin","doi":"10.4467/16890027AP.17.027.7829","DOIUrl":"https://doi.org/10.4467/16890027AP.17.027.7829","url":null,"abstract":"Naegleria is well recognized for primary amoebic meningoencephalitis which always results into death. To date there is not a single report demonstrating molecular identification of Naegleria from water resources of Pakistan thus the aim of the proposed study. Here, in total 135 various water samples (like domestic tap water, municipal water, sea water, well water, tube well water, canal water, boring water, sewage water, lake water and stream water) were collected across Pakistan and evaluated for Naegleria species. Naegleria australiensis (pathogenic) and Naegleria lovaniensis (non pathogenic) were isolated on non nutrient agar plates and were further identified by PCR and sequencing. To best of our knowledge we have described for the first time the isolation and molecular identification of thermotolerant pathogenic and non pathogenic Naegleria species from diverse water samples including drinking water across Pakistan. The presence of pathogenic Naegleria species in diverse water samples may add the health threat to the community.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"2017 1","pages":"317-322"},"PeriodicalIF":0.5,"publicationDate":"2017-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46564592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-14DOI: 10.4467/16890027AP.17.021.7823
E. Gentekaki, Chitchai Chantangsi
Opaline is an unusual group of protists, characterized by the presence of flagella covering their whole body. They reside in the intestinal tracts of various animals, most notably amphibians. While there is a wealth of data regarding the morphological features of opalines, molecular data are extremely sparse. Consequently, the extent of diversity of this understudied group remains unknown. Here, we examine opalines from the intestinal tract of the amphibian Hoplobatrachus rugulosus in Thailand. We provide micrographs obtained from light and scanning electron microscopy of various opalinid morphotypes. Furthermore, we enrich the database of opaline sequences by providing new molecular data of the small subunit ribosomal DNA gene of these species. In our phylogenetic analyses, the newly derived sequences form a cluster sister to Protoopalina.
{"title":"Low-level genetic diversity of opalinid morphotypes from the digestive tract of Hoplobatrachus rugulosus (Batrachia, Amphibia) in Thailand","authors":"E. Gentekaki, Chitchai Chantangsi","doi":"10.4467/16890027AP.17.021.7823","DOIUrl":"https://doi.org/10.4467/16890027AP.17.021.7823","url":null,"abstract":"Opaline is an unusual group of protists, characterized by the presence of flagella covering their whole body. They reside in the intestinal tracts of various animals, most notably amphibians. While there is a wealth of data regarding the morphological features of opalines, molecular data are extremely sparse. Consequently, the extent of diversity of this understudied group remains unknown. Here, we examine opalines from the intestinal tract of the amphibian Hoplobatrachus rugulosus in Thailand. We provide micrographs obtained from light and scanning electron microscopy of various opalinid morphotypes. Furthermore, we enrich the database of opaline sequences by providing new molecular data of the small subunit ribosomal DNA gene of these species. In our phylogenetic analyses, the newly derived sequences form a cluster sister to Protoopalina.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"2017 1","pages":"235-243"},"PeriodicalIF":0.5,"publicationDate":"2017-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43141243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-14DOI: 10.4467/16890027AP.17.024.7826
M. Yaman
Chrysomela populi L. (Coleoptera; Chrysomelidae) is one of the most serious pests affecting poplars. Chemical control is the most widely known suppressive method against this pest. This method is not acceptable in urban forests because of their proximity to people. Entomopathogenic organisms are environmentally friendly control agents and suppress the pest populations under natural conditions. In the present study, the occurrence and distribution of a new entomopathogenic protist, a neogregarine, Ophryocystis anatoliensis (Apicomplexa) in C. populi populations and a member of the family Chrysomelidae is presented for the first time. In total, 90 of 2185 C. populi adults and larvae collected from 16 localities during three years (from 2013 to 2015) were found to be infected by this pathogen. Neogregarine infection was observed in 14 of 16 investigated C. populi populations. The infection was variable between the populations and years. These results confirm that the neogregarine pathogen has a high dispersal potential through the C. populi populations and can be an effective natural biological suppressing factor on the pest populations.
{"title":"Distribution and occurrence of the neogregarine pathogen, Ophryocystis anatoliensis (Apicomplexa) in populations of Chrysomela populi L. (Coleoptera: Chrysomelidae)","authors":"M. Yaman","doi":"10.4467/16890027AP.17.024.7826","DOIUrl":"https://doi.org/10.4467/16890027AP.17.024.7826","url":null,"abstract":"Chrysomela populi L. (Coleoptera; Chrysomelidae) is one of the most serious pests affecting poplars. Chemical control is the most widely known suppressive method against this pest. This method is not acceptable in urban forests because of their proximity to people. Entomopathogenic organisms are environmentally friendly control agents and suppress the pest populations under natural conditions. In the present study, the occurrence and distribution of a new entomopathogenic protist, a neogregarine, Ophryocystis anatoliensis (Apicomplexa) in C. populi populations and a member of the family Chrysomelidae is presented for the first time. In total, 90 of 2185 C. populi adults and larvae collected from 16 localities during three years (from 2013 to 2015) were found to be infected by this pathogen. Neogregarine infection was observed in 14 of 16 investigated C. populi populations. The infection was variable between the populations and years. These results confirm that the neogregarine pathogen has a high dispersal potential through the C. populi populations and can be an effective natural biological suppressing factor on the pest populations.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"56 1","pages":"283-288"},"PeriodicalIF":0.5,"publicationDate":"2017-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45034217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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