Pub Date : 2023-12-22Epub Date: 2023-12-21DOI: 10.26444/aaem/176816
Jolanta Małgorzata Zdybel, Jacek Karamon, Jacek Sroka, Angelina Wójcik-Fatla, Piotr Skowron, Katarzyna Kowalczyk, Jan Jadczyszyn, Tomasz Cencek
Introduction and objective: The hygienic status of arable soils in most developed countries has been unknown. In the presented study, a preliminary investigation was undertaken to determine the contamination with eggs of parasitic nematodes in the soil of arable fields in Poland. The aim of the study was to determine whether such contamination is common enough to constitute a significant problem and what factors may influence it.
Material and methods: The study was conducted in 5 Polish provinces from autumn 2021 to spring 2022. The provinces differed significantly in terms of the area of agricultural land, agricultural suitability, type of soil, scale of cattle and pig breeding, production of manure and slurry, and the use of manures and organic fertilizers for fertilization. A total of 133 soil samples were collected. Parasitological examination of soil samples was carried out using the PN-Z-19006 method [1], with confirmed high sensitivity.
Results: Parasite eggs were found in a total of 67 samples, of which 56 samples contained eggs of roundworms of the genus Ascaris (an average of 3.29 eggs/100 g of soil), 23 contained eggs of whipworms (an average of 1.22 eggs/100 g), and 3 contained eggs of Toxocara (1 egg/100 g).
Conclusions: Differences in the percentage of positive samples were found depending on the period in which the samples were taken. The percentage of positive samples collected in autumn (53.57%) was higher than the percentage of positive samples collected in spring (48.05%). Similarly, the average number of eggs of in positive samples collected in autumn (3.43 eggs/100 g) was higher than the average number of eggs in samples collected in spring (2.90 eggs/100 g). Differences in the percentage of positive samples were also found depending on the region of origin of the samples.
{"title":"Parasitological contamination of arable soil in selected regions of Poland - preliminary study.","authors":"Jolanta Małgorzata Zdybel, Jacek Karamon, Jacek Sroka, Angelina Wójcik-Fatla, Piotr Skowron, Katarzyna Kowalczyk, Jan Jadczyszyn, Tomasz Cencek","doi":"10.26444/aaem/176816","DOIUrl":"10.26444/aaem/176816","url":null,"abstract":"<p><strong>Introduction and objective: </strong>The hygienic status of arable soils in most developed countries has been unknown. In the presented study, a preliminary investigation was undertaken to determine the contamination with eggs of parasitic nematodes in the soil of arable fields in Poland. The aim of the study was to determine whether such contamination is common enough to constitute a significant problem and what factors may influence it.</p><p><strong>Material and methods: </strong>The study was conducted in 5 Polish provinces from autumn 2021 to spring 2022. The provinces differed significantly in terms of the area of agricultural land, agricultural suitability, type of soil, scale of cattle and pig breeding, production of manure and slurry, and the use of manures and organic fertilizers for fertilization. A total of 133 soil samples were collected. Parasitological examination of soil samples was carried out using the PN-Z-19006 method [1], with confirmed high sensitivity.</p><p><strong>Results: </strong>Parasite eggs were found in a total of 67 samples, of which 56 samples contained eggs of roundworms of the genus <i>Ascaris</i> (an average of 3.29 eggs/100 g of soil), 23 contained eggs of whipworms (an average of 1.22 eggs/100 g), and 3 contained eggs of Toxocara (1 egg/100 g).</p><p><strong>Conclusions: </strong>Differences in the percentage of positive samples were found depending on the period in which the samples were taken. The percentage of positive samples collected in autumn (53.57%) was higher than the percentage of positive samples collected in spring (48.05%). Similarly, the average number of eggs of in positive samples collected in autumn (3.43 eggs/100 g) was higher than the average number of eggs in samples collected in spring (2.90 eggs/100 g). Differences in the percentage of positive samples were also found depending on the region of origin of the samples.</p>","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139049732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-22Epub Date: 2023-06-14DOI: 10.26444/aaem/167934
Iwona Gładysz, Agnieszka Sikora, Małgorzata Wójtowicz-Bobin, Adam Szepeluk, Renata Matuszewska, Marta Bartosik, Jan Kazimierz Karczewski
Introduction and objective: Legionella bacteria are commonly found in natural aquatic environments such as rivers, lakes, ponds and hot springs. Legionella infection occurs through the inhalation of water-air aerosol generated, for example, by showers or hot tubs. The most common species responsible for infection is Legionella pneumophila, which can cause Pontiac fever, and Legionnaires' disease, as well as a rare extrapulmonary form. The aim of the study's is to assess the susceptibility of Legionella pneumophila bacteria isolated from water systems of public buildings in Poland to antibiotics and chemotherapeutic agents used in the treatment of Legionellosis pneumonia.
Material and methods: A total of 100 L. pneumophila strains isolated from public buildings, such as hospitals and water recreation facilities, were used for the study. The drug sensitivity of the following antibiotics was determined: erythromycin, azithromycin, ciprofloxacin, levofloxacin, rifampicin, trimethoprim-sulfamethoxazole and tetracycline. Mean MIC50 and MIC90 values were read using accepted standards.
Results: The highest mean MIC value was obtained for tetracycline 6,130+/-0,353 μg/ml (with a range from 1,500 μg/ml to 16,000 μg/ml. In contrast, the lowest MIC was recorded with rifampicin: 0.020+/-0.037 μg/ml (with a range from 0.016 μg/ml to 0.380 μg/ml).
Conclusions: The lowest biocidal concentration was found for levofloxacin, the highest for tetracycline. The highest MIC50 and MIC90 values were found for tetracycline and the lowest for rifampicin. The highest biocidal values were found for azithromycin and the lowest for tetracycline.
{"title":"Antibiotic sensitivity of environmental Legionella pneumophila strains isolated in Poland.","authors":"Iwona Gładysz, Agnieszka Sikora, Małgorzata Wójtowicz-Bobin, Adam Szepeluk, Renata Matuszewska, Marta Bartosik, Jan Kazimierz Karczewski","doi":"10.26444/aaem/167934","DOIUrl":"10.26444/aaem/167934","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Legionella bacteria are commonly found in natural aquatic environments such as rivers, lakes, ponds and hot springs. Legionella infection occurs through the inhalation of water-air aerosol generated, for example, by showers or hot tubs. The most common species responsible for infection is Legionella pneumophila, which can cause Pontiac fever, and Legionnaires' disease, as well as a rare extrapulmonary form. The aim of the study's is to assess the susceptibility of Legionella pneumophila bacteria isolated from water systems of public buildings in Poland to antibiotics and chemotherapeutic agents used in the treatment of Legionellosis pneumonia.</p><p><strong>Material and methods: </strong>A total of 100 L. pneumophila strains isolated from public buildings, such as hospitals and water recreation facilities, were used for the study. The drug sensitivity of the following antibiotics was determined: erythromycin, azithromycin, ciprofloxacin, levofloxacin, rifampicin, trimethoprim-sulfamethoxazole and tetracycline. Mean MIC50 and MIC90 values were read using accepted standards.</p><p><strong>Results: </strong>The highest mean MIC value was obtained for tetracycline 6,130+/-0,353 μg/ml (with a range from 1,500 μg/ml to 16,000 μg/ml. In contrast, the lowest MIC was recorded with rifampicin: 0.020+/-0.037 μg/ml (with a range from 0.016 μg/ml to 0.380 μg/ml).</p><p><strong>Conclusions: </strong>The lowest biocidal concentration was found for levofloxacin, the highest for tetracycline. The highest MIC50 and MIC90 values were found for tetracycline and the lowest for rifampicin. The highest biocidal values were found for azithromycin and the lowest for tetracycline.</p>","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43506222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-22Epub Date: 2023-07-14DOI: 10.26444/aaem/168414
Beata Świątkowska, Marta Szkiela, Radosław Zajdel, Kamila Gworys, Dorota Kaleta
Introduction and objective: Shift work increases the risk of breast cancer, but the mechanisms is still under discussion. This study evaluates the relationship between breast cancer and shift work on the basis of overweight and obesity among postmenopausal women.
Material and methods: We examined this association using data from a case-control study carried between 2015 and 2019. The study involved 111 postmenopausal women with breast cancer and the same number of control participants. A self-reporting questionnaire was used for data collection. Multivariate logistic regression was conducted to find correlations between variables and determine the strength of relationships.
Results: A 2.65-fold risk of breast cancer (OR=2.65; 95% CI: 1.34-5.22) was found among shift work women, compared with postmenopausal women not performing shift work. The association was modified by body mass index, showing a risk rate 9.84 times higher (OR=9.84; 95% CI: 2.14-45.19) among shift work and overweight women, compared to non-overweight women who had never been shift workers.
Conclusions: About 49% of controls and 72% of cases had ever worked in a job that required shift work. The risk of breast cancer in postmenopausal women is associated with shift work, especially among overweight women. Some preventive measures to reduce the risk of breast cancer, in particular regarding a healthy lifestyle and weight control in this group of working women, should be implemented.
{"title":"Shift work, body mass index and associated breast cancer risks in postmenopausal women.","authors":"Beata Świątkowska, Marta Szkiela, Radosław Zajdel, Kamila Gworys, Dorota Kaleta","doi":"10.26444/aaem/168414","DOIUrl":"10.26444/aaem/168414","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Shift work increases the risk of breast cancer, but the mechanisms is still under discussion. This study evaluates the relationship between breast cancer and shift work on the basis of overweight and obesity among postmenopausal women.</p><p><strong>Material and methods: </strong>We examined this association using data from a case-control study carried between 2015 and 2019. The study involved 111 postmenopausal women with breast cancer and the same number of control participants. A self-reporting questionnaire was used for data collection. Multivariate logistic regression was conducted to find correlations between variables and determine the strength of relationships.</p><p><strong>Results: </strong>A 2.65-fold risk of breast cancer (OR=2.65; 95% CI: 1.34-5.22) was found among shift work women, compared with postmenopausal women not performing shift work. The association was modified by body mass index, showing a risk rate 9.84 times higher (OR=9.84; 95% CI: 2.14-45.19) among shift work and overweight women, compared to non-overweight women who had never been shift workers.</p><p><strong>Conclusions: </strong>About 49% of controls and 72% of cases had ever worked in a job that required shift work. The risk of breast cancer in postmenopausal women is associated with shift work, especially among overweight women. Some preventive measures to reduce the risk of breast cancer, in particular regarding a healthy lifestyle and weight control in this group of working women, should be implemented.</p>","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43888476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-22Epub Date: 2023-05-08DOI: 10.26444/aaem/163600
Marta Sajdakowska, Krystyna Gutkowska, Jerzy Gębski, Małgorzata Kosicka-Gębska, Andrzej Gantner
Introduction and objective: Research is required to better understand the variables related to the frequency and cohesiveness of family meals. The aim of the study was to determine the association of eating meals with the family with other types of eating behaviours, sedentary behaviours, and physical activity based on a self-reported questionnaire carried out on a sample of the Polish population of rural adolescents.
Material and methods: The analysis was performed among adolescents living in rural areas who participated in a survey conducted within the framework of the 'Trzymaj formę!' ('Keep Fit!') programme on eating behaviours. The survey questionnaire was completed by 2,362 teenagers aged 13-16.
Results: Generally, positive evidence in terms of eating behaviours resulted in a higher likelihood of eating meals together with family. Not eating breakfast resulted in a decreased chance of eating meals together with the family. The results show that with regard to the use of computers at weekends, in the case of using them for a rather limited time, i.e. from 0.5-2 hours a day, there was a greater chance of having meals together.
Conclusions: The results confirm the role of educational programmes in increasing the chances of sharing meals with the family, which confirms the validity of conducting this type of education among children and adolescents. It can be also concluded that the more positive eating behaviours of the surveyed junior high students aged 13-16, consisting in more frequent consumption of fruit, greater consumption of cereal products, and more water consumed during the day, is proof of the positive influence of parents on the nutritional behaviour of their children. This is consistent with good intra-family relationships reflected in a greater tendency to spend time together over a family meal.
{"title":"Association between family meals vs. diet quality and leisure activities of young rural residents.","authors":"Marta Sajdakowska, Krystyna Gutkowska, Jerzy Gębski, Małgorzata Kosicka-Gębska, Andrzej Gantner","doi":"10.26444/aaem/163600","DOIUrl":"10.26444/aaem/163600","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Research is required to better understand the variables related to the frequency and cohesiveness of family meals. The aim of the study was to determine the association of eating meals with the family with other types of eating behaviours, sedentary behaviours, and physical activity based on a self-reported questionnaire carried out on a sample of the Polish population of rural adolescents.</p><p><strong>Material and methods: </strong>The analysis was performed among adolescents living in rural areas who participated in a survey conducted within the framework of the 'Trzymaj formę!' ('Keep Fit!') programme on eating behaviours. The survey questionnaire was completed by 2,362 teenagers aged 13-16.</p><p><strong>Results: </strong>Generally, positive evidence in terms of eating behaviours resulted in a higher likelihood of eating meals together with family. Not eating breakfast resulted in a decreased chance of eating meals together with the family. The results show that with regard to the use of computers at weekends, in the case of using them for a rather limited time, i.e. from 0.5-2 hours a day, there was a greater chance of having meals together.</p><p><strong>Conclusions: </strong>The results confirm the role of educational programmes in increasing the chances of sharing meals with the family, which confirms the validity of conducting this type of education among children and adolescents. It can be also concluded that the more positive eating behaviours of the surveyed junior high students aged 13-16, consisting in more frequent consumption of fruit, greater consumption of cereal products, and more water consumed during the day, is proof of the positive influence of parents on the nutritional behaviour of their children. This is consistent with good intra-family relationships reflected in a greater tendency to spend time together over a family meal.</p>","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42964002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-22Epub Date: 2023-05-29DOI: 10.26444/aaem/165980
Elżbieta Biernat, Monika Piątkowska
Introduction and objective: Relief from routine physical tasks leads to increasingly sedentary behaviour (SB) - a risk factor for non-communicable chronic diseases. It is necessary to gather interdisciplinary knowledge about the possibilities of its reduction. The aim of the study is to analyse SB and its conditions among Polish white-collar and blue-collar workers.
Material and methods: The study is based on data retrieved from a large-scale survey used to collect information on the physical activity of Polish society. The data were gathered on a representative sample of Poles at working age ≤69 years old in 2017 (n=2,131). The Polish long version of the International Physical Activity Questionnaire (IPAQ-LF) was used. Statistical inference was based on non-parametric (U-Mann Whitney and Kruskal-Wallis) tests.
Results: The total average sitting time of the respondents was 1,958.5 min./week. White-collar workers are more likely (p 7.5 h/day (20.2% vs 9%), and blue-collar - ≤4.5 h/day (56.5% vs 42.1%). On a weekday, white-collar workers are sitting significantly longer. The bigger the place of residence, the longer the time sitting in both groups (p=0.000). The time of sitting on a non-working day does not differ among white-collar (221.4 min./day) and blue-collar workers (230.4 min./day). White-collar workers sit longer in vehicles (p <0.0001): 482.4 vs blue-collar workers 326.8 min./week.
Conclusions: The results suggest a change in Polish recommendations regarding the SB. Blue-collar and white-collar workers need support from the State policy in this matter and need guidance from pro-health programmes. It is necessary to intensify coherent, interdisciplinary and intersectoral activities aimed at creating environments that effectively counteract SB in the place of work and residence, especially in the larger agglomerations.
{"title":"Sedentary behaviour as a lifestyle risk factor in public health - Evidence of white-collar and blue-collar workers from Poland.","authors":"Elżbieta Biernat, Monika Piątkowska","doi":"10.26444/aaem/165980","DOIUrl":"10.26444/aaem/165980","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Relief from routine physical tasks leads to increasingly sedentary behaviour (SB) - a risk factor for non-communicable chronic diseases. It is necessary to gather interdisciplinary knowledge about the possibilities of its reduction. The aim of the study is to analyse SB and its conditions among Polish white-collar and blue-collar workers.</p><p><strong>Material and methods: </strong>The study is based on data retrieved from a large-scale survey used to collect information on the physical activity of Polish society. The data were gathered on a representative sample of Poles at working age ≤69 years old in 2017 (n=2,131). The Polish long version of the International Physical Activity Questionnaire (IPAQ-LF) was used. Statistical inference was based on non-parametric (U-Mann Whitney and Kruskal-Wallis) tests.</p><p><strong>Results: </strong>The total average sitting time of the respondents was 1,958.5 min./week. White-collar workers are more likely (p 7.5 h/day (20.2% vs 9%), and blue-collar - ≤4.5 h/day (56.5% vs 42.1%). On a weekday, white-collar workers are sitting significantly longer. The bigger the place of residence, the longer the time sitting in both groups (p=0.000). The time of sitting on a non-working day does not differ among white-collar (221.4 min./day) and blue-collar workers (230.4 min./day). White-collar workers sit longer in vehicles (p <0.0001): 482.4 vs blue-collar workers 326.8 min./week.</p><p><strong>Conclusions: </strong>The results suggest a change in Polish recommendations regarding the SB. Blue-collar and white-collar workers need support from the State policy in this matter and need guidance from pro-health programmes. It is necessary to intensify coherent, interdisciplinary and intersectoral activities aimed at creating environments that effectively counteract SB in the place of work and residence, especially in the larger agglomerations.</p>","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44495452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Nowak-Terpiłowska, Romuald Górski, Małgorzata Marszałek, S. Wosiński, R. Przesmycki, M. Bugaj, L. Nowosielski, Mikołaj Baranowski, Joanna Zeyland
{"title":"Effects of 2.4 GHz radiofrequency electromagnetic field (RF-EMF) on glioblastoma cells (U -118 MG)","authors":"A. Nowak-Terpiłowska, Romuald Górski, Małgorzata Marszałek, S. Wosiński, R. Przesmycki, M. Bugaj, L. Nowosielski, Mikołaj Baranowski, Joanna Zeyland","doi":"10.26444/aaem/176405","DOIUrl":"https://doi.org/10.26444/aaem/176405","url":null,"abstract":"","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138951395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tomasz Prystupa, Dariusz Sagan, J. Kocki, Tomasz Kocki, Rafał Szymanowski, Jacek Bogucki
Introduction and Objective. Lung cancer is the most common malignant tumour. More than 80% of all diagnosed cases are non-small cell carcinoma which can be effectively treated by radical resection. Despite significant progress in the field of diagnostic and therapeutic methods, the results of lung cancer treatment are still unsatisfactory. Lung cancer is detected relatively late, which leads to an unfavourable prognosis. Kynurenine aminotransferases are an important element of the kynurenine pathway of tryptophan metabolism, which has recently aroused great interest from the aspect of possible use as a target point of personalized therapies in malignant tumours.The aim of the study was to analyze the expression of the selected gene of kynurenine aminotransferases GOT 2 at the mRNA level in peripheral blood leukocytes of patients with lung cancer. Materials and method. The mRNA expression of the GOT 2 gene was tested on blood samples from 50 patients treated surgically for non-small cell lung cancer.The control group consisted of 15 healthy individuals.The determination of mRNA expression of the GOT 2 gene was performed using the real-time PCR method.The GAPDH gene was used as the endogenous reference level. Results. The mRNA expression of the GOT2 gene on the 6th day after surgery was statistically significantly lower than before surgery (p = 0,05). In the study group, the average LogRQ mRNA expression of the GOT2 gene before the procedure was 0.192082±0.292174 in woman. This was statistically significantly higher than in men whose average LogRQ mRNA expression of the GOT2 gene before the procedure was 0.004210±0.235065 (p=0.0183). Conclusions. Surgical resection of lung cancer results in inhibition of GOT2 mRNA expression in leukocytes. Further studies are expected to show whether it may be used as a target point for personalized therapies in lung cancer.
{"title":"Surgical resection of lung cancer inhibits mRNA expression of GOT2 gene encoding kynurenine aminotransferase in leukocytes","authors":"Tomasz Prystupa, Dariusz Sagan, J. Kocki, Tomasz Kocki, Rafał Szymanowski, Jacek Bogucki","doi":"10.26444/aaem/176813","DOIUrl":"https://doi.org/10.26444/aaem/176813","url":null,"abstract":"Introduction and Objective. Lung cancer is the most common malignant tumour. More than 80% of all diagnosed cases are non-small cell carcinoma which can be effectively treated by radical resection. Despite significant progress in the field of diagnostic and therapeutic methods, the results of lung cancer treatment are still unsatisfactory. Lung cancer is detected relatively late, which leads to an unfavourable prognosis. Kynurenine aminotransferases are an important element of the kynurenine pathway of tryptophan metabolism, which has recently aroused great interest from the aspect of possible use as a target point of personalized therapies in malignant tumours.The aim of the study was to analyze the expression of the selected gene of kynurenine aminotransferases GOT 2 at the mRNA level in peripheral blood leukocytes of patients with lung cancer. Materials and method. The mRNA expression of the GOT 2 gene was tested on blood samples from 50 patients treated surgically for non-small cell lung cancer.The control group consisted of 15 healthy individuals.The determination of mRNA expression of the GOT 2 gene was performed using the real-time PCR method.The GAPDH gene was used as the endogenous reference level. Results. The mRNA expression of the GOT2 gene on the 6th day after surgery was statistically significantly lower than before surgery (p = 0,05). In the study group, the average LogRQ mRNA expression of the GOT2 gene before the procedure was 0.192082±0.292174 in woman. This was statistically significantly higher than in men whose average LogRQ mRNA expression of the GOT2 gene before the procedure was 0.004210±0.235065 (p=0.0183). Conclusions. Surgical resection of lung cancer results in inhibition of GOT2 mRNA expression in leukocytes. Further studies are expected to show whether it may be used as a target point for personalized therapies in lung cancer.","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138948371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wenfang Long, mingxue Li, Yi Gao, Yu Zhang, xinyu Yuan, Haimei Ye, Hairong Huang, wenjuan Liang, rongguang Zhang, Guankui Du
Objective. The aim of the study was to explore the correlation between characteristics of microbial community, pathogenic bacteria and high-risk antibiotic-resistant genes, between coastal beaches and a multi-warm-blooded host, as well as to determine potential species biomarkers for faecal source contamination on tropical coastal beaches in China. Methods. The ‘One-Health’ approach was used in a microbiological study of beaches and warm-blooded hosts. The microbial.community was analyzed using 16S rRNA gene amplicons and shotgun metagenomics on Illumina NovaSeq. Results. The Chao, Simpson, Shannon, and ACE indices of non-salt beach were greater than those of salt beaches at the genus and OTU levels ( P < 0.001). Bacteroidota, Halanaerobiaeota, Cyanobacteria
{"title":"Microbial community, pathogenic bacteria and high-risk anti-biotic resistance genes at two tropical coastal beaches adjacent to villages in Hainan, China","authors":"Wenfang Long, mingxue Li, Yi Gao, Yu Zhang, xinyu Yuan, Haimei Ye, Hairong Huang, wenjuan Liang, rongguang Zhang, Guankui Du","doi":"10.26444/aaem/176090","DOIUrl":"https://doi.org/10.26444/aaem/176090","url":null,"abstract":"Objective. The aim of the study was to explore the correlation between characteristics of microbial community, pathogenic bacteria and high-risk antibiotic-resistant genes, between coastal beaches and a multi-warm-blooded host, as well as to determine potential species biomarkers for faecal source contamination on tropical coastal beaches in China. Methods. The ‘One-Health’ approach was used in a microbiological study of beaches and warm-blooded hosts. The microbial.community was analyzed using 16S rRNA gene amplicons and shotgun metagenomics on Illumina NovaSeq. Results. The Chao, Simpson, Shannon, and ACE indices of non-salt beach were greater than those of salt beaches at the genus and OTU levels ( P < 0.001). Bacteroidota, Halanaerobiaeota, Cyanobacteria","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138949702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jerzy Mosiewicz, Klaudia Brożyna-Tkaczyk, Elżbieta Reichert, Wojciech Myśliński, L. Panasiuk, Andrzej Jaroszyński, Barbara Mosiewicz-Madejska
{"title":"Selected routine laboratory tests in the clinical assessment of patients with obstructive sleep apnea","authors":"Jerzy Mosiewicz, Klaudia Brożyna-Tkaczyk, Elżbieta Reichert, Wojciech Myśliński, L. Panasiuk, Andrzej Jaroszyński, Barbara Mosiewicz-Madejska","doi":"10.26444/aaem/177205","DOIUrl":"https://doi.org/10.26444/aaem/177205","url":null,"abstract":"","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138950703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Work in agriculture carries the risk of trauma as a result of mechanization (farm equipment and machinery), location of cultivated areas, and multi-functionality. In agriculture, the hands were the most affected parts of the body which sustained injury. Successful suturing of a finger initially disqualified from replantation is rare. The case is presented of a 55-year-old patient who had a non-work-related finger amputation. In a surgical centre not belonging to the reference ones in plastic surgery in Poland, a doctor with six months of surgical experience performed the replantation without vascular reconstruction (the amputated distal part of the finger served as a biological dressing). Despite the lack of vascular reconstruction and unfavourable prognosis, the amputated fragment of the finger maintained a partial blood supply. Subsequent flap surgery in the plastic surgery department, and rehabilitation of the patient due to finger contracture, led to functional recovery of the finger.
{"title":"Rehabilitation after replantation of a non-work-related finger amputation without vascular anastosmosis – case report","authors":"Justyna Frasuńska, Dominika Kostka, Beata Tarnacka","doi":"10.26444/aaem/176089","DOIUrl":"https://doi.org/10.26444/aaem/176089","url":null,"abstract":"Work in agriculture carries the risk of trauma as a result of mechanization (farm equipment and machinery), location of cultivated areas, and multi-functionality. In agriculture, the hands were the most affected parts of the body which sustained injury. Successful suturing of a finger initially disqualified from replantation is rare. The case is presented of a 55-year-old patient who had a non-work-related finger amputation. In a surgical centre not belonging to the reference ones in plastic surgery in Poland, a doctor with six months of surgical experience performed the replantation without vascular reconstruction (the amputated distal part of the finger served as a biological dressing). Despite the lack of vascular reconstruction and unfavourable prognosis, the amputated fragment of the finger maintained a partial blood supply. Subsequent flap surgery in the plastic surgery department, and rehabilitation of the patient due to finger contracture, led to functional recovery of the finger.","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139169423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}