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Parasitological contamination of arable soil in selected regions of Poland - preliminary study. 波兰部分地区耕地土壤的寄生虫污染--初步研究。
IF 1.7 4区 医学 Q2 Agricultural and Biological Sciences Pub Date : 2023-12-22 Epub Date: 2023-12-21 DOI: 10.26444/aaem/176816
Jolanta Małgorzata Zdybel, Jacek Karamon, Jacek Sroka, Angelina Wójcik-Fatla, Piotr Skowron, Katarzyna Kowalczyk, Jan Jadczyszyn, Tomasz Cencek

Introduction and objective: The hygienic status of arable soils in most developed countries has been unknown. In the presented study, a preliminary investigation was undertaken to determine the contamination with eggs of parasitic nematodes in the soil of arable fields in Poland. The aim of the study was to determine whether such contamination is common enough to constitute a significant problem and what factors may influence it.

Material and methods: The study was conducted in 5 Polish provinces from autumn 2021 to spring 2022. The provinces differed significantly in terms of the area of agricultural land, agricultural suitability, type of soil, scale of cattle and pig breeding, production of manure and slurry, and the use of manures and organic fertilizers for fertilization. A total of 133 soil samples were collected. Parasitological examination of soil samples was carried out using the PN-Z-19006 method [1], with confirmed high sensitivity.

Results: Parasite eggs were found in a total of 67 samples, of which 56 samples contained eggs of roundworms of the genus Ascaris (an average of 3.29 eggs/100 g of soil), 23 contained eggs of whipworms (an average of 1.22 eggs/100 g), and 3 contained eggs of Toxocara (1 egg/100 g).

Conclusions: Differences in the percentage of positive samples were found depending on the period in which the samples were taken. The percentage of positive samples collected in autumn (53.57%) was higher than the percentage of positive samples collected in spring (48.05%). Similarly, the average number of eggs of in positive samples collected in autumn (3.43 eggs/100 g) was higher than the average number of eggs in samples collected in spring (2.90 eggs/100 g). Differences in the percentage of positive samples were also found depending on the region of origin of the samples.

引言和目的:大多数发达国家耕地土壤的卫生状况一直不为人知。本研究对波兰耕地土壤中寄生线虫虫卵的污染情况进行了初步调查。研究的目的是确定这种污染是否普遍到足以构成重大问题,以及哪些因素可能对其产生影响:研究于 2021 年秋季至 2022 年春季在波兰 5 个省进行。这些省份在农业用地面积、农业适宜性、土壤类型、养牛和养猪规模、粪肥和泥浆生产以及使用粪肥和有机肥施肥等方面存在显著差异。共采集了 133 个土壤样本。采用 PN-Z-19006 方法[1]对土壤样本进行了寄生虫学检测,结果表明该方法灵敏度高:结果:共在 67 个样本中发现寄生虫卵,其中 56 个样本含有蛔虫卵(平均每 100 克土壤中有 3.29 个虫卵),23 个样本含有鞭虫卵(平均每 100 克土壤中有 1.22 个虫卵),3 个样本含有弓形虫卵(每 100 克土壤中有 1 个虫卵):不同时期采集的样本阳性率不同。秋季采集的阳性样本比例(53.57%)高于春季采集的阳性样本比例(48.05%)。同样,秋季采集的阳性样本的平均卵数(每 100 克 3.43 个卵)也高于春季采集的样本的平均卵数(每 100 克 2.90 个卵)。根据样本来源地区的不同,阳性样本的百分比也存在差异。
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引用次数: 0
Antibiotic sensitivity of environmental Legionella pneumophila strains isolated in Poland. 波兰环境嗜肺军团菌菌株的抗生素敏感性
IF 1.7 4区 医学 Q2 Agricultural and Biological Sciences Pub Date : 2023-12-22 Epub Date: 2023-06-14 DOI: 10.26444/aaem/167934
Iwona Gładysz, Agnieszka Sikora, Małgorzata Wójtowicz-Bobin, Adam Szepeluk, Renata Matuszewska, Marta Bartosik, Jan Kazimierz Karczewski

Introduction and objective: Legionella bacteria are commonly found in natural aquatic environments such as rivers, lakes, ponds and hot springs. Legionella infection occurs through the inhalation of water-air aerosol generated, for example, by showers or hot tubs. The most common species responsible for infection is Legionella pneumophila, which can cause Pontiac fever, and Legionnaires' disease, as well as a rare extrapulmonary form. The aim of the study's is to assess the susceptibility of Legionella pneumophila bacteria isolated from water systems of public buildings in Poland to antibiotics and chemotherapeutic agents used in the treatment of Legionellosis pneumonia.

Material and methods: A total of 100 L. pneumophila strains isolated from public buildings, such as hospitals and water recreation facilities, were used for the study. The drug sensitivity of the following antibiotics was determined: erythromycin, azithromycin, ciprofloxacin, levofloxacin, rifampicin, trimethoprim-sulfamethoxazole and tetracycline. Mean MIC50 and MIC90 values were read using accepted standards.

Results: The highest mean MIC value was obtained for tetracycline 6,130+/-0,353 μg/ml (with a range from 1,500 μg/ml to 16,000 μg/ml. In contrast, the lowest MIC was recorded with rifampicin: 0.020+/-0.037 μg/ml (with a range from 0.016 μg/ml to 0.380 μg/ml).

Conclusions: The lowest biocidal concentration was found for levofloxacin, the highest for tetracycline. The highest MIC50 and MIC90 values were found for tetracycline and the lowest for rifampicin. The highest biocidal values were found for azithromycin and the lowest for tetracycline.

引言和目的。军团菌常见于河流、湖泊、池塘和温泉等天然水生环境中。军团菌感染是通过吸入例如淋浴或热水浴缸产生的水-空气气溶胶而发生的。最常见的感染种类是嗜肺军团菌,它可引起庞蒂亚克热和军团病,以及一种罕见的肺外感染。该研究的目的是评估从波兰公共建筑供水系统中分离出来的嗜肺军团菌对用于治疗肺炎军团菌的抗生素和化疗药物的敏感性。材料和方法。从医院和水上娱乐设施等公共建筑中分离的嗜肺乳杆菌共100株用于研究。对红霉素、阿奇霉素、环丙沙星、左氧氟沙星、利福平、甲氧苄啶-磺胺甲恶唑、四环素等抗生素进行敏感性测定。采用公认标准读取MIC50和MIC90平均值。结果。四环素的平均MIC值最高,为6130 +/- 0.353 μg/ml(范围为1500 ~ 16000 μg/ml)。相比之下,利福平的最低MIC为0.020±0.037 μg/ml(范围为0.016 ~ 0.380 μg/ml)。结论。左氧氟沙星杀菌剂浓度最低,四环素杀菌剂浓度最高。MIC50和MIC90值最高的是四环素,最低的是利福平。阿奇霉素的杀虫效果最好,四环素的杀虫效果最差。
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引用次数: 0
Shift work, body mass index and associated breast cancer risks in postmenopausal women. 倒班工作、体重指数和绝经后妇女相关乳腺癌风险
IF 1.7 4区 医学 Q2 Agricultural and Biological Sciences Pub Date : 2023-12-22 Epub Date: 2023-07-14 DOI: 10.26444/aaem/168414
Beata Świątkowska, Marta Szkiela, Radosław Zajdel, Kamila Gworys, Dorota Kaleta

Introduction and objective: Shift work increases the risk of breast cancer, but the mechanisms is still under discussion. This study evaluates the relationship between breast cancer and shift work on the basis of overweight and obesity among postmenopausal women.

Material and methods: We examined this association using data from a case-control study carried between 2015 and 2019. The study involved 111 postmenopausal women with breast cancer and the same number of control participants. A self-reporting questionnaire was used for data collection. Multivariate logistic regression was conducted to find correlations between variables and determine the strength of relationships.

Results: A 2.65-fold risk of breast cancer (OR=2.65; 95% CI: 1.34-5.22) was found among shift work women, compared with postmenopausal women not performing shift work. The association was modified by body mass index, showing a risk rate 9.84 times higher (OR=9.84; 95% CI: 2.14-45.19) among shift work and overweight women, compared to non-overweight women who had never been shift workers.

Conclusions: About 49% of controls and 72% of cases had ever worked in a job that required shift work. The risk of breast cancer in postmenopausal women is associated with shift work, especially among overweight women. Some preventive measures to reduce the risk of breast cancer, in particular regarding a healthy lifestyle and weight control in this group of working women, should be implemented.

引言和目的。轮班工作会增加患乳腺癌的风险,但其机制仍在讨论中。本研究在绝经后妇女超重和肥胖的基础上评估乳腺癌与轮班工作之间的关系。材料和方法。我们使用2015年至2019年进行的病例对照研究的数据来检验这种关联。该研究涉及111名绝经后乳腺癌妇女和相同数量的对照组。采用自我报告问卷进行数据收集。进行多元逻辑回归,以发现变量之间的相关性,并确定关系的强度。结果。乳腺癌风险为2.65倍(OR=2.65;95% CI: 1.34-5.22),与绝经后不进行轮班工作的妇女相比。这种关联被身体质量指数修正,显示风险率高出9.84倍(OR=9.84;95% CI: 2.14-45.19),与从未做过轮班工作的非超重女性相比。结论。大约49%的对照组和72%的病例曾经从事过需要倒班的工作。绝经后妇女患乳腺癌的风险与轮班工作有关,尤其是超重妇女。应该采取一些预防措施,减少患乳腺癌的风险,特别是在这一职业妇女群体中采取健康的生活方式和控制体重。
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引用次数: 0
Association between family meals vs. diet quality and leisure activities of young rural residents. 农村青年居民家庭聚餐、饮食质量与休闲活动的关系
IF 1.7 4区 医学 Q2 Agricultural and Biological Sciences Pub Date : 2023-12-22 Epub Date: 2023-05-08 DOI: 10.26444/aaem/163600
Marta Sajdakowska, Krystyna Gutkowska, Jerzy Gębski, Małgorzata Kosicka-Gębska, Andrzej Gantner

Introduction and objective: Research is required to better understand the variables related to the frequency and cohesiveness of family meals. The aim of the study was to determine the association of eating meals with the family with other types of eating behaviours, sedentary behaviours, and physical activity based on a self-reported questionnaire carried out on a sample of the Polish population of rural adolescents.

Material and methods: The analysis was performed among adolescents living in rural areas who participated in a survey conducted within the framework of the 'Trzymaj formę!' ('Keep Fit!') programme on eating behaviours. The survey questionnaire was completed by 2,362 teenagers aged 13-16.

Results: Generally, positive evidence in terms of eating behaviours resulted in a higher likelihood of eating meals together with family. Not eating breakfast resulted in a decreased chance of eating meals together with the family. The results show that with regard to the use of computers at weekends, in the case of using them for a rather limited time, i.e. from 0.5-2 hours a day, there was a greater chance of having meals together.

Conclusions: The results confirm the role of educational programmes in increasing the chances of sharing meals with the family, which confirms the validity of conducting this type of education among children and adolescents. It can be also concluded that the more positive eating behaviours of the surveyed junior high students aged 13-16, consisting in more frequent consumption of fruit, greater consumption of cereal products, and more water consumed during the day, is proof of the positive influence of parents on the nutritional behaviour of their children. This is consistent with good intra-family relationships reflected in a greater tendency to spend time together over a family meal.

引言和目标。需要进行研究,以更好地了解与家庭用餐频率和凝聚力相关的变量。该研究的目的是根据对波兰农村青少年样本进行的自我报告问卷,确定与家人一起吃饭与其他类型的饮食行为、久坐行为和体育活动的关系。材料和方法。这项分析是在农村地区的青少年中进行的,他们参加了在“Trzymaj formÉ!”框架内进行的一项调查(“保持健康!”)饮食行为计划。调查问卷由2362名13-16岁的青少年完成。后果一般来说,饮食行为方面的积极证据会导致与家人一起用餐的可能性更高。不吃早餐会减少与家人一起用餐的机会。结果表明,就周末使用电脑而言,在使用电脑的时间相当有限的情况下,即每天0.5-2小时,一起吃饭的机会更大。结论。研究结果证实了教育方案在增加与家人共享膳食的机会方面的作用,这证实了在儿童和青少年中进行这种教育的有效性。还可以得出结论,13-16岁的受访初中生更积极的饮食行为,包括更频繁地食用水果、更多地食用谷物产品和白天更多地喝水,证明了父母对孩子营养行为的积极影响。这与良好的家庭内部关系是一致的,这反映在更倾向于花时间在一起吃饭。
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引用次数: 0
Sedentary behaviour as a lifestyle risk factor in public health - Evidence of white-collar and blue-collar workers from Poland. 久坐行为是公共卫生中的生活方式风险因素——波兰白领和蓝领工人的证据
IF 1.7 4区 医学 Q2 Agricultural and Biological Sciences Pub Date : 2023-12-22 Epub Date: 2023-05-29 DOI: 10.26444/aaem/165980
Elżbieta Biernat, Monika Piątkowska

Introduction and objective: Relief from routine physical tasks leads to increasingly sedentary behaviour (SB) - a risk factor for non-communicable chronic diseases. It is necessary to gather interdisciplinary knowledge about the possibilities of its reduction. The aim of the study is to analyse SB and its conditions among Polish white-collar and blue-collar workers.

Material and methods: The study is based on data retrieved from a large-scale survey used to collect information on the physical activity of Polish society. The data were gathered on a representative sample of Poles at working age ≤69 years old in 2017 (n=2,131). The Polish long version of the International Physical Activity Questionnaire (IPAQ-LF) was used. Statistical inference was based on non-parametric (U-Mann Whitney and Kruskal-Wallis) tests.

Results: The total average sitting time of the respondents was 1,958.5 min./week. White-collar workers are more likely (p 7.5 h/day (20.2% vs 9%), and blue-collar - ≤4.5 h/day (56.5% vs 42.1%). On a weekday, white-collar workers are sitting significantly longer. The bigger the place of residence, the longer the time sitting in both groups (p=0.000). The time of sitting on a non-working day does not differ among white-collar (221.4 min./day) and blue-collar workers (230.4 min./day). White-collar workers sit longer in vehicles (p <0.0001): 482.4 vs blue-collar workers 326.8 min./week.

Conclusions: The results suggest a change in Polish recommendations regarding the SB. Blue-collar and white-collar workers need support from the State policy in this matter and need guidance from pro-health programmes. It is necessary to intensify coherent, interdisciplinary and intersectoral activities aimed at creating environments that effectively counteract SB in the place of work and residence, especially in the larger agglomerations.

导言和目标:从日常体力劳动中解脱出来会导致越来越多的久坐行为(SB)--这是导致非传染性慢性疾病的一个风险因素。有必要收集有关减少久坐的可能性的跨学科知识。本研究旨在分析波兰白领和蓝领工人的久坐行为及其状况:本研究基于一项大规模调查所获得的数据,该调查用于收集波兰社会体育活动方面的信息。数据收集对象为2017年工作年龄小于69岁的波兰人(n=2131)。使用的是波兰语长版国际体育活动问卷(IPAQ-LF)。统计推断基于非参数(U-Mann Whitney 和 Kruskal-Wallis)检验:受访者的总平均久坐时间为 1958.5 分钟/周。白领更有可能坐 7.5 小时/天(20.2% 对 9%),蓝领--≤4.5 小时/天(56.5% 对 42.1%)。在工作日,白领坐的时间明显更长。居住地越大,两组人坐的时间越长(P=0.000)。白领(221.4 分钟/天)和蓝领(230.4 分钟/天)在非工作日的坐着时间没有差异。白领工人在车内坐的时间更长(p 结论:白领工人在车内坐的时间更长:这些结果表明,波兰关于坐姿支撑的建议有所改变。蓝领工人和白领工人在这个问题上需要得到国家政策的支持,需要得到有利于健康计划的指导。有必要加强协调、跨学科和跨部门的活动,以便在工作和居住地,特别是在较大的城市群,创造有效抵制可吸入颗粒物的环境。
{"title":"Sedentary behaviour as a lifestyle risk factor in public health - Evidence of white-collar and blue-collar workers from Poland.","authors":"Elżbieta Biernat, Monika Piątkowska","doi":"10.26444/aaem/165980","DOIUrl":"10.26444/aaem/165980","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Relief from routine physical tasks leads to increasingly sedentary behaviour (SB) - a risk factor for non-communicable chronic diseases. It is necessary to gather interdisciplinary knowledge about the possibilities of its reduction. The aim of the study is to analyse SB and its conditions among Polish white-collar and blue-collar workers.</p><p><strong>Material and methods: </strong>The study is based on data retrieved from a large-scale survey used to collect information on the physical activity of Polish society. The data were gathered on a representative sample of Poles at working age ≤69 years old in 2017 (n=2,131). The Polish long version of the International Physical Activity Questionnaire (IPAQ-LF) was used. Statistical inference was based on non-parametric (U-Mann Whitney and Kruskal-Wallis) tests.</p><p><strong>Results: </strong>The total average sitting time of the respondents was 1,958.5 min./week. White-collar workers are more likely (p 7.5 h/day (20.2% vs 9%), and blue-collar - ≤4.5 h/day (56.5% vs 42.1%). On a weekday, white-collar workers are sitting significantly longer. The bigger the place of residence, the longer the time sitting in both groups (p=0.000). The time of sitting on a non-working day does not differ among white-collar (221.4 min./day) and blue-collar workers (230.4 min./day). White-collar workers sit longer in vehicles (p <0.0001): 482.4 vs blue-collar workers 326.8 min./week.</p><p><strong>Conclusions: </strong>The results suggest a change in Polish recommendations regarding the SB. Blue-collar and white-collar workers need support from the State policy in this matter and need guidance from pro-health programmes. It is necessary to intensify coherent, interdisciplinary and intersectoral activities aimed at creating environments that effectively counteract SB in the place of work and residence, especially in the larger agglomerations.</p>","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44495452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of 2.4 GHz radiofrequency electromagnetic field (RF-EMF) on glioblastoma cells (U -118 MG) 2.4 GHz 射频电磁场(RF-EMF)对胶质母细胞瘤细胞(U -118 MG)的影响
IF 1.7 4区 医学 Q2 Agricultural and Biological Sciences Pub Date : 2023-12-21 DOI: 10.26444/aaem/176405
A. Nowak-Terpiłowska, Romuald Górski, Małgorzata Marszałek, S. Wosiński, R. Przesmycki, M. Bugaj, L. Nowosielski, Mikołaj Baranowski, Joanna Zeyland
{"title":"Effects of 2.4 GHz radiofrequency electromagnetic field (RF-EMF) on glioblastoma cells (U -118 MG)","authors":"A. Nowak-Terpiłowska, Romuald Górski, Małgorzata Marszałek, S. Wosiński, R. Przesmycki, M. Bugaj, L. Nowosielski, Mikołaj Baranowski, Joanna Zeyland","doi":"10.26444/aaem/176405","DOIUrl":"https://doi.org/10.26444/aaem/176405","url":null,"abstract":"","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138951395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Surgical resection of lung cancer inhibits mRNA expression of GOT2 gene encoding kynurenine aminotransferase in leukocytes 肺癌手术切除可抑制白细胞中编码犬尿氨酸氨基转移酶的 GOT2 基因的 mRNA 表达
IF 1.7 4区 医学 Q2 Agricultural and Biological Sciences Pub Date : 2023-12-21 DOI: 10.26444/aaem/176813
Tomasz Prystupa, Dariusz Sagan, J. Kocki, Tomasz Kocki, Rafał Szymanowski, Jacek Bogucki
Introduction and Objective. Lung cancer is the most common malignant tumour. More than 80% of all diagnosed cases are non-small cell carcinoma which can be effectively treated by radical resection. Despite significant progress in the field of diagnostic and therapeutic methods, the results of lung cancer treatment are still unsatisfactory. Lung cancer is detected relatively late, which leads to an unfavourable prognosis. Kynurenine aminotransferases are an important element of the kynurenine pathway of tryptophan metabolism, which has recently aroused great interest from the aspect of possible use as a target point of personalized therapies in malignant tumours.The aim of the study was to analyze the expression of the selected gene of kynurenine aminotransferases GOT 2 at the mRNA level in peripheral blood leukocytes of patients with lung cancer. Materials and method. The mRNA expression of the GOT 2 gene was tested on blood samples from 50 patients treated surgically for non-small cell lung cancer.The control group consisted of 15 healthy individuals.The determination of mRNA expression of the GOT 2 gene was performed using the real-time PCR method.The GAPDH gene was used as the endogenous reference level. Results. The mRNA expression of the GOT2 gene on the 6th day after surgery was statistically significantly lower than before surgery (p = 0,05). In the study group, the average LogRQ mRNA expression of the GOT2 gene before the procedure was 0.192082±0.292174 in woman. This was statistically significantly higher than in men whose average LogRQ mRNA expression of the GOT2 gene before the procedure was 0.004210±0.235065 (p=0.0183). Conclusions. Surgical resection of lung cancer results in inhibition of GOT2 mRNA expression in leukocytes. Further studies are expected to show whether it may be used as a target point for personalized therapies in lung cancer.
导言和目的。肺癌是最常见的恶性肿瘤。在所有确诊病例中,80% 以上为非小细胞癌,可通过根治性切除术进行有效治疗。尽管在诊断和治疗方法领域取得了重大进展,但肺癌的治疗效果仍不尽如人意。肺癌的发现时间相对较晚,导致预后不良。犬尿氨酸氨基转移酶是色氨酸代谢过程中犬尿氨酸途径的重要组成部分,最近引起了人们的极大兴趣,因为它可能被用作恶性肿瘤个性化疗法的靶点。材料和方法。采用实时 PCR 法检测 GOT 2 基因 mRNA 的表达,以 GAPDH 基因作为内源性参考水平。结果显示术后第 6 天,GOT2 基因的 mRNA 表达量明显低于术前(P = 0.05)。在研究组中,女性术前 GOT2 基因的平均 LogRQ mRNA 表达量为 0.192082±0.292174。这在统计学上明显高于男性,男性术前 GOT2 基因的平均 LogRQ mRNA 表达量为 0.004210±0.235065 (p=0.0183)。结论肺癌手术切除会抑制白细胞中 GOT2 mRNA 的表达。进一步的研究有望显示它是否可作为肺癌个性化疗法的靶点。
{"title":"Surgical resection of lung cancer inhibits mRNA expression of GOT2 gene encoding kynurenine aminotransferase in leukocytes","authors":"Tomasz Prystupa, Dariusz Sagan, J. Kocki, Tomasz Kocki, Rafał Szymanowski, Jacek Bogucki","doi":"10.26444/aaem/176813","DOIUrl":"https://doi.org/10.26444/aaem/176813","url":null,"abstract":"Introduction and Objective. Lung cancer is the most common malignant tumour. More than 80% of all diagnosed cases are non-small cell carcinoma which can be effectively treated by radical resection. Despite significant progress in the field of diagnostic and therapeutic methods, the results of lung cancer treatment are still unsatisfactory. Lung cancer is detected relatively late, which leads to an unfavourable prognosis. Kynurenine aminotransferases are an important element of the kynurenine pathway of tryptophan metabolism, which has recently aroused great interest from the aspect of possible use as a target point of personalized therapies in malignant tumours.The aim of the study was to analyze the expression of the selected gene of kynurenine aminotransferases GOT 2 at the mRNA level in peripheral blood leukocytes of patients with lung cancer. Materials and method. The mRNA expression of the GOT 2 gene was tested on blood samples from 50 patients treated surgically for non-small cell lung cancer.The control group consisted of 15 healthy individuals.The determination of mRNA expression of the GOT 2 gene was performed using the real-time PCR method.The GAPDH gene was used as the endogenous reference level. Results. The mRNA expression of the GOT2 gene on the 6th day after surgery was statistically significantly lower than before surgery (p = 0,05). In the study group, the average LogRQ mRNA expression of the GOT2 gene before the procedure was 0.192082±0.292174 in woman. This was statistically significantly higher than in men whose average LogRQ mRNA expression of the GOT2 gene before the procedure was 0.004210±0.235065 (p=0.0183). Conclusions. Surgical resection of lung cancer results in inhibition of GOT2 mRNA expression in leukocytes. Further studies are expected to show whether it may be used as a target point for personalized therapies in lung cancer.","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138948371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microbial community, pathogenic bacteria and high-risk anti-biotic resistance genes at two tropical coastal beaches adjacent to villages in Hainan, China 中国海南毗邻村庄的两个热带沿海海滩的微生物群落、致病菌和高风险抗生素耐药基因
IF 1.7 4区 医学 Q2 Agricultural and Biological Sciences Pub Date : 2023-12-21 DOI: 10.26444/aaem/176090
Wenfang Long, mingxue Li, Yi Gao, Yu Zhang, xinyu Yuan, Haimei Ye, Hairong Huang, wenjuan Liang, rongguang Zhang, Guankui Du
Objective. The aim of the study was to explore the correlation between characteristics of microbial community, pathogenic bacteria and high-risk antibiotic-resistant genes, between coastal beaches and a multi-warm-blooded host, as well as to determine potential species biomarkers for faecal source contamination on tropical coastal beaches in China. Methods. The ‘One-Health’ approach was used in a microbiological study of beaches and warm-blooded hosts. The microbial.community was analyzed using 16S rRNA gene amplicons and shotgun metagenomics on Illumina NovaSeq. Results. The Chao, Simpson, Shannon, and ACE indices of non-salt beach were greater than those of salt beaches at the genus and OTU levels ( P < 0.001). Bacteroidota, Halanaerobiaeota, Cyanobacteria
研究目的本研究旨在探讨中国沿海海滩与多温血宿主之间微生物群落特征、致病菌和高风险抗生素耐药基因之间的相关性,并确定中国热带沿海海滩粪便源污染的潜在物种生物标志物。研究方法采用 "同一健康 "方法对海滩和温血宿主进行微生物研究。使用 16S rRNA 基因扩增子和 Illumina NovaSeq 上的猎枪元基因组学分析微生物群落。结果显示在属和 OTU 水平上,非盐滩的 Chao 指数、Simpson 指数、Shannon 指数和 ACE 指数均高于盐滩(P < 0.001)。类细菌群, Halanaerobiaeota, 蓝藻群
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引用次数: 0
Selected routine laboratory tests in the clinical assessment of patients with obstructive sleep apnea 阻塞性睡眠呼吸暂停患者临床评估中的部分常规实验室检查
IF 1.7 4区 医学 Q2 Agricultural and Biological Sciences Pub Date : 2023-12-21 DOI: 10.26444/aaem/177205
Jerzy Mosiewicz, Klaudia Brożyna-Tkaczyk, Elżbieta Reichert, Wojciech Myśliński, L. Panasiuk, Andrzej Jaroszyński, Barbara Mosiewicz-Madejska
{"title":"Selected routine laboratory tests in the clinical assessment of patients with obstructive sleep apnea","authors":"Jerzy Mosiewicz, Klaudia Brożyna-Tkaczyk, Elżbieta Reichert, Wojciech Myśliński, L. Panasiuk, Andrzej Jaroszyński, Barbara Mosiewicz-Madejska","doi":"10.26444/aaem/177205","DOIUrl":"https://doi.org/10.26444/aaem/177205","url":null,"abstract":"","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138950703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rehabilitation after replantation of a non-work-related finger amputation without vascular anastosmosis – case report 无血管吻合的非工作相关手指截肢再植术后的康复--病例报告
IF 1.7 4区 医学 Q2 Agricultural and Biological Sciences Pub Date : 2023-12-20 DOI: 10.26444/aaem/176089
Justyna Frasuńska, Dominika Kostka, Beata Tarnacka
Work in agriculture carries the risk of trauma as a result of mechanization (farm equipment and machinery), location of cultivated areas, and multi-functionality. In agriculture, the hands were the most affected parts of the body which sustained injury. Successful suturing of a finger initially disqualified from replantation is rare. The case is presented of a 55-year-old patient who had a non-work-related finger amputation. In a surgical centre not belonging to the reference ones in plastic surgery in Poland, a doctor with six months of surgical experience performed the replantation without vascular reconstruction (the amputated distal part of the finger served as a biological dressing). Despite the lack of vascular reconstruction and unfavourable prognosis, the amputated fragment of the finger maintained a partial blood supply. Subsequent flap surgery in the plastic surgery department, and rehabilitation of the patient due to finger contracture, led to functional recovery of the finger.
由于机械化(农用设备和机械)、耕地位置和多功能性,从事农业工作有遭受创伤的风险。在农业生产中,手是受伤最严重的部位。最初被取消再植资格的手指很少能成功缝合。本病例涉及一名 55 岁的患者,他的手指被非手术截肢。在波兰一家不属于整形外科参考中心的外科中心,一名只有六个月手术经验的医生在没有进行血管重建的情况下进行了再植手术(截取的手指远端部分用作生物敷料)。尽管没有进行血管重建,预后也不乐观,但断指部分仍保持了部分血液供应。随后在整形外科进行了皮瓣手术,患者因手指挛缩进行了康复治疗,手指功能得以恢复。
{"title":"Rehabilitation after replantation of a non-work-related finger amputation without vascular anastosmosis – case report","authors":"Justyna Frasuńska, Dominika Kostka, Beata Tarnacka","doi":"10.26444/aaem/176089","DOIUrl":"https://doi.org/10.26444/aaem/176089","url":null,"abstract":"Work in agriculture carries the risk of trauma as a result of mechanization (farm equipment and machinery), location of cultivated areas, and multi-functionality. In agriculture, the hands were the most affected parts of the body which sustained injury. Successful suturing of a finger initially disqualified from replantation is rare. The case is presented of a 55-year-old patient who had a non-work-related finger amputation. In a surgical centre not belonging to the reference ones in plastic surgery in Poland, a doctor with six months of surgical experience performed the replantation without vascular reconstruction (the amputated distal part of the finger served as a biological dressing). Despite the lack of vascular reconstruction and unfavourable prognosis, the amputated fragment of the finger maintained a partial blood supply. Subsequent flap surgery in the plastic surgery department, and rehabilitation of the patient due to finger contracture, led to functional recovery of the finger.","PeriodicalId":50970,"journal":{"name":"Annals of Agricultural and Environmental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139169423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Annals of Agricultural and Environmental Medicine
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