Purpose
The widespread adoption of mechanical harvesters in tea plantations has intensified soil compaction, posing a major abiotic constraint on tea plant growth. Elucidating the physiological mechanisms underlying such stress is critical for developing effective mitigation strategies.
Methods
A four-year field experiment comparing tillage (T), no-tillage (NT), and mechanical compaction (MC) treatments on plant physiological, metabolic, and transcriptional responses of tea root and leaf tissues were employed in this study.
Results
T treatment significantly enhanced soil nutrient availability, with available nitrogen, phosphorus, potassium, iron, and manganese concentrations 19.32–109.16 % higher than those under MC treatment. MC reduced plant height, suppressed root architecture development, and induced antioxidant defenses. Both roots and leaves showed increased malondialdehyde (MDA) levels and elevated activities of CAT and POD by 18.23–77.01 %, indicating oxidative stress mitigation. Metabolomic profiling identified flavonoid biosynthesis as the dominant response pathway under compaction, with key flavonoid metabolites including epicatechin, luteoforol, and phloretin, accumulating markedly in both tissues. Hormonal analysis showed increased levels of gibberellin A7 and brassinolide under MC, and organ-specific expression of regulatory genes (e.g., CHS, DFR, IAA, PYL) coordinated these metabolic adjustments.
Conclusions
This study demonstrates that soil compaction severely limits tea plant growth while triggering defense-related metabolic regulation. In contrast, tillage enhances nutrient availability and promotes plant development, whereas compaction induces oxidative stress and stimulates flavonoid-mediated defense responses. Overall, these findings provide mechanistic insights into tea plant adaptation to soil compaction and offer valuable guidance for sustainable management practices in tea cultivation.
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