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RNA interference-mediated functional analysis of vitellogenin and vitellogenin receptor in the predatory mite Neoseiulus californicus RNA 干扰介导的捕食螨 Neoseiulus californicus 体内卵黄素和卵黄素受体的功能分析
IF 1.2 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-03-25 DOI: 10.11158/saa.29.3.3
Rui Zhu, Tianci Yi, Run-dong Liu, Dao-chao Jin
Neoseiulus californicus is a key natural predator of spider mites and small arthropod pests. Although vitellogenin (Vg) and vitellogenin receptor (VgR) genes are important regulators of oocyte maturation, egg formation, and embryonic development in spider mites and ticks, their role in phytoseiid mites is less understood. Investigating the reproductive mechanism of predatory mites is crucial for understanding their reproduction and evolution. In this study, three Vg genes and one VgR gene were identified, cloned, and characterized in N. californicus. Vg1, Vg2, Vg3, and VgR mRNAs were 6066, 5728, 4515, and 5331 bp long, encoding proteins comprising 1851, 1849, 1471, and 1722 amino acids, respectively. Temporal expression analysis revealed elevated Vg1, Vg2, and VgR levels in fertilized female adult mites, whereas Vg3 exhibited peak expression during the developmental stage. RNA interference–mediated suppression of Vg1 and Vg2 resulted in 14.66% and 11.59% decreases in fecundity, respectively. Vg1 silencing notably reduced egg hatching rate and female offspring ratio. In contrast, Vg3 and VgR silencing had no significant impact on fecundity and egg hatching rate. These findings provide insights into the regulatory roles of Vg and VgR in predatory mite reproduction, offering a theoretical foundation for understanding their evolution and reproductive strategies.
加利福尼亚新薮蛛是蜘蛛螨和小型节肢动物害虫的主要天敌。虽然卵黄原素(Vg)和卵黄原素受体(VgR)基因是蜘蛛螨和蜱卵母细胞成熟、卵形成和胚胎发育的重要调节因子,但它们在植食螨中的作用却鲜为人知。研究捕食螨的繁殖机制对于了解它们的繁殖和进化至关重要。在这项研究中,发现、克隆并鉴定了加利福尼亚螨(N. californicus)的三个 Vg 基因和一个 VgR 基因。Vg1、Vg2、Vg3和VgR mRNA长分别为6066、5728、4515和5331 bp,编码的蛋白质分别包含1851、1849、1471和1722个氨基酸。时间表达分析表明,受精雌成螨的 Vg1、Vg2 和 VgR 水平升高,而 Vg3 则在发育阶段达到表达峰值。RNA 干扰介导的 Vg1 和 Vg2 抑制分别导致受精率下降 14.66% 和 11.59%。Vg1 的沉默显著降低了卵孵化率和雌性后代比例。相比之下,Vg3 和 VgR 的沉默对繁殖力和卵孵化率没有显著影响。这些发现深入揭示了Vg和VgR在捕食螨繁殖过程中的调控作用,为理解捕食螨的进化和繁殖策略提供了理论基础。
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引用次数: 0
The effects of cyflumetofen on life history traits and population parameters of Amblyseius swirskii 三氟氯氰菊酯对鞘氨蝶啶生活史特征和种群参数的影响
IF 1.2 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-03-08 DOI: 10.11158/saa.29.3.2
M. Havasi, K. Kheradmand
Even as new chemical substances show promise as selectivity for controlling pests due to their high effectiveness in inhibiting pests, their side effects on non-target organisms must nevertheless be evaluated before they can be included in integrated pest management systems. We examined both lethal and sublethal (LC10 and LC30) effects of cyflumetofen on the predatory potential of the Amblyseius swirskii Athias Henriot (Acari: Phytoseiidae) which        is among the most dominant mite predators in tropical agricultural agroecosystems. The experiments were performed under laboratory conditions at 25 ± 2°C, 65 ± 5% relative humidity (RH), and a photoperiod of 16:8 (L: D) hours. Results were analyzed based on age-stage, two-sex life table analysis. The data showed that sublethal concentrations of the pesticides suppress fecundity, total life span (both female and male individuals), and the intrinsic and finite rate of increase significantly. The result show, the fecundity in the LC10, LC30, and control was 14.97, 11.87, and 17.28, eggs/female for LC10 and LC30, respectively, showing significant differences. The values of the intrinsic rate of increase (r) on the above-mentioned concentrations were 0.1333, 0.1166, and 0.1430 day-1, respectively. Consequently, according to the data, it seems that the selected acaricide is not suitable to use with A. swiriskii in integrated pest management programs due to their unfavorable effects on the population parameters of this predatory mite.
即使新化学物质因其抑制害虫的高效性而显示出控制害虫的选择性前景,但在将其纳入害虫综合管理系统之前,必须评估其对非目标生物的副作用。我们研究了氟虫腈对热带农业生态系统中最主要的螨类捕食者之一 Amblyseius swirskii Athias Henriot(螨类:Phytoseiidae)的致死和亚致死(LC10 和 LC30)影响。实验在实验室条件下进行,温度为 25 ± 2°C,相对湿度(RH)为 65 ± 5%,光周期为 16:8 (L: D) 小时。实验结果根据年龄阶段、双性别生命表分析得出。数据显示,亚致死浓度的农药会显著抑制繁殖力、总寿命(包括雌性和雄性个体)、固有增长率和有限增长率。结果表明,LC10、LC30 和对照组的繁殖力分别为 14.97、11.87 和 17.28 个卵/雌性,差异明显。上述浓度的内在增长率(r)值分别为 0.1333、0.1166 和 0.1430 天-1。因此,根据这些数据,所选杀螨剂似乎不适合在害虫综合治理方案中与 A. swiriskii 一起使用,因为它们对这种捕食性螨虫的种群参数有不利影响。
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引用次数: 0
Two new species of Limnesia (Acari, Hydrachnidiae, Lebertioidea) from China, with the descriptions of two instars 来自中国的两个 Limnesia(蛔虫纲,蛛形纲,Lebertioidea)新种,并描述了两个雏形
IF 1.2 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-03-08 DOI: 10.11158/saa.29.3.1
Hai-tao Li, Xin-yao Gu, Yu-hao Zhang, D. Jin, Jian-jun Guo
A brief introduction of Chinese Limnesiidae taxonomy is provided. Adults of two new species, Limnesia (Limnesia) lupingae Li & Guo sp. nov. and Limnesia (Limnesia) macrophthalma Li & Guo sp. nov., from Heilongjiang and Hubei provinces respectively, are described in detail. Subtle structures are recorded with scanning electron micrographs. In addition, deutonymphs of two new species are also described in detail and compared with the adults in discussion.
本文简要介绍了中国菱角科的分类。详细描述了分别产于黑龙江省和湖北省的两个新种--Limnesia (Limnesia) lupingae Li & Guo sp.nov.和Limnesia (Limnesia) macrophthalma Li & Guo sp.nov.的成虫。用扫描电子显微照记录了微妙的结构。此外,还详细描述了两个新种的幼虫,并与成虫进行了比较讨论。
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引用次数: 0
Enhancing the efficiency of egg collection of the astigmatid mite Carpoglyphus lactis (Acari: Carpoglyphidae) as a diet for predatory mites 提高作为捕食螨食物的星螨 Carpoglyphus lactis(Acari: Carpoglyphidae)的采卵效率
IF 1.2 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-29 DOI: 10.11158/saa.29.2.14
Zhenguo Liu, Keshi Zhang, Zhi-qiang Zhang
N/A
不适用
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引用次数: 0
Contribution to knowledge of the oribatid mite genus Protozetes (Acari, Oribatida, Microzetidae), with description of a new species from Guatemala 对原螨属(Acari, Oribatida, Microzetidae)知识的贡献,并描述了危地马拉的一个新物种
IF 1.2 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-29 DOI: 10.11158/saa.29.2.13
S. Ermilov
The genus Protozetes (Oribatida, Microzetidae) is recorded in Guatemala for the first time. A new species—Protozetes janosbaloghi sp. nov.—is described from damp litter in an alpine cloud forest in Guatemala. It differs from all known species of the genus in the number of the interlamellar apophyses, the morphology of the head of the bothridial seta, the ornamentation of the pteromorph, and the length of the interlamellar and notogastral setae. A revised generic diagnosis is presented. An identification key and data on distribution and habitat of the known species of Protozetes are provided.
首次在危地马拉记录到 Protozetes 属(Oribatida,Microzetidae)。新种--Protozetes janosbaloghi sp.它与该属所有已知种的不同之处包括:膜间节的数量、两鳃刚毛头部的形态、翼形体的装饰以及膜间刚毛和喙刚毛的长度。本文提出了经修订的属种诊断。此外,还提供了一个识别钥匙以及已知原虫物种的分布和栖息地数据。
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引用次数: 0
Rubroscirus karnatakaensis sp. nov. and redescription of R. sinensis Fan (Acari: Cunaxidae) from plants in India 印度植物中的Rubroscirus karnatakaensis sp.
IF 1.2 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-29 DOI: 10.11158/saa.29.2.11
R. Hiremath, A. Khaustov, N. Srinivasa, Channegowda Chinnamade Gowda, Anna Jose
Two species of Rubroscirus (Acari: Cunaxidae) were collected from the Eastern dry zone of Karnataka, India. Females and males of Rubroscirus sinensis Fan, 1992, collected from five different species of plants, are recorded for the first time from India and redescribed. The female of Rubroscirus karnatakaensis sp. nov. collected on three species of plants is described. This bring the total number of Rubroscirus species from India to three.
从印度卡纳塔克邦东部干旱地区采集到两种Rubroscirus(Acari:Cunaxidae)。从 5 种不同植物上采集的 Rubroscirus sinensis 的雌性和雄性是首次从印度记录并重新描述。在三种植物上采集到的 Rubroscirus karnatakaensis sp.这使得来自印度的 Rubroscirus 种类总数达到 3 种。
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引用次数: 0
Development and reproductive potential of Oulenziella bakeri Hughes (Acari: Winterschmidtiidae) at different constant temperatures Oulenziella bakeri Hughes(Acari:Winterschmidtiidae)在不同恒温条件下的发育和繁殖潜力
IF 1.2 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-16 DOI: 10.11158/saa.29.2.8
Zhao-hua Zeng, Shi-yong Xie, Ling-hong Lin, Jian-cheng Huang, De-yi Yu, Jie Ji
Oulenziella bakeri Hughes is a promising alternative prey for mass rearing of predatory mites. However, little is known regarding the effects of different temperatures on the population growth of O. bakeri. In the present study, the developmental duration, survival rate, longevity, fecundity, and sex ratio of O. bakeri were observed under laboratory conditions at 16°C, 20°C, 24°C, 28°C, and 32°C. The developmental duration and whole generation time of O. bakeri shortened with increasing temperature, except for the protonymphal duration, which was the shortest at 24°C. The maximum fecundity of O. bakeri was recorded at 28°C (176.73 eggs/female) but did not significantly differ from that at 24°C (173.80 eggs/female). The sex ratio for the progeny of O. bakeri was female-biased, and showed no significant difference among 16°C, 20°C, and 24°C, but a significant decrease as the temperature further increased. Life table analysis indicated that the net reproductive rate of O. bakeri was highest at 28°C, with a value of 140.37. Furthermore, the intrinsic rate of increase (rm) of O. bakeri ranged from 0.12 to 0.38, and increased with the temperature. Our findings suggested that temperatures of 24°C and 28°C were most suitable among the tested temperatures for rearing O. bakeri.
Hughes Oulenziella bakeri 是大量饲养捕食螨的一种很有前途的替代性猎物。然而,人们对不同温度对 O. bakeri 种群增长的影响知之甚少。本研究在 16°C、20°C、24°C、28°C 和 32°C 的实验室条件下观察了 O. bakeri 的发育期、存活率、寿命、繁殖力和性别比。随着温度的升高,O. bakeri 的发育持续时间和整个世代时间都缩短了,只有原肠持续时间在 24°C 时最短。28°C时的卵数最多(176.73枚/雌性),但与24°C时的卵数(173.80枚/雌性)相比没有显著差异。雌性偏多,16°C、20°C 和 24°C 三种温度下的性别比差异不大,但随着温度的升高,性别比显著下降。生命表分析表明,O. bakeri 的净生殖率在 28°C 时最高,为 140.37。此外,O. bakeri 的内在增长率(rm)在 0.12 至 0.38 之间,并随温度升高而增加。我们的研究结果表明,在所测试的温度中,24°C 和 28°C 最适合饲养 O. bakeri。
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引用次数: 0
Two new species of Oripodoidea (Acari, Oribatida) from Nepal 来自尼泊尔的两个新种 Oripodoidea(蛔虫,Oribatida
IF 1.2 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-16 DOI: 10.11158/saa.29.2.9
S. Ermilov, Jochen Martens
Two new species of oribatid mites of the superfamily Oripodoidea—Peloribates (Peloribates) ausobskyi sp. nov. (Haplozetidae) and Scheloribates (Perscheloribates) smetanai sp. nov. (Scheloribatidae)—are described from litter and upper soil stratum of Rhododendron–Lithocarpus mountain forest from eastern Nepal. Identification keys to the known species of Peloribates (Peloribates) and Scheloribates (Perscheloribates) from Nepal are provided.
描述了尼泊尔东部杜鹃-石松山林落叶层和上土层中的两个新种--Oripodoidea超科的眶螨--Peloribates (Peloribates) ausobskyi sp.报告还提供了尼泊尔已知的Peloribates和Scheloribates的识别钥匙。
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引用次数: 0
Complementary description of three species of the subgenus Anthoseius De Leon (Acari: Phytoseiidae) from India 印度 Anthoseius De Leon 亚属(蛔虫:Phytoseiidae)三个种的补充描述
IF 1.2 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-16 DOI: 10.11158/saa.29.2.10
Anna Jose, Ismail Döker, Channegowda Chinnamade Gowda, R. Hiremath
Taxonomy of the subgenus Anthoseius De Leon (Acari: Phytoseiidae) has become challenging and often causing confusions among taxonomists. We here provide complementary descriptions of three species, T. (A.) bengalensis Karmakar et al., T. (A.) bulbosis Karmakar et al., and T. (A.) serrulatus Ehara, based on the specimens collected from Karnataka, India. During the identification of our specimens, we observed several important morphological differences between the new specimens and the original descriptions of the first two species. The morphological differences include number of dorsal solenostomes (absence or presence of gd5), position of preanal pores, chelicera dentition including position of teeth, shape of calyx of spermatheca, and leg chaetotaxy including number of setae on genu II, number of macrosetae and shape of ventral setae. Since these characters are crucial for phytoseiid taxonomy, we decided to examine the type specimens of these two species. Our examination on their holotypes confirmed that the morphological characters mentioned above are different than those reported in their original descriptions, but identical to the complementary descriptions provided here. In addition, T. (A.) serrulatus has not been recognized by several acarologists and repeatedly described under different names. We proposed five new and three suspected synonymies for this species. Lastly, we also provided the first description of males of T. (A.) bengalensis.
Anthoseius De Leon 亚属(蛔虫纲:Phytoseiidae)的分类已变得极具挑战性,常常引起分类学家的混淆。(A.) bengalensis Karmakar et al.、T. (A.) bulbosis Karmakar et al.和T. (A.) serrulatus Ehara这三个种的补充描述。在对标本进行鉴定的过程中,我们观察到新标本与前两个物种的原始描述之间存在一些重要的形态差异。这些形态差异包括背侧独角虫的数量(gd5 的存在与否)、肛前孔的位置、螯齿(包括牙齿的位置)、精鞘花萼的形状以及腿部的序列(包括属Ⅱ上刚毛的数量、大刚毛的数量和腹侧刚毛的形状)。由于这些特征对植物鞘蝶的分类至关重要,我们决定对这两个物种的模式标本进行研究。我们对这两个物种的模式标本进行了研究,结果表明上述形态特征与原始描述中的特征不同,但与本文的补充描述相同。此外,T. (A.) serrulatus 并未得到多位刺桐学家的认可,并多次以不同的名称进行描述。我们为该物种提出了五个新异名和三个疑似异名。最后,我们还首次描述了 T. (A.) bengalensis 的雄性。
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引用次数: 0
Redescription of Scirula papilata Lin, 1997 (Acariformes: Cunaxidae), with the first description of males for the species 重新描述 Scirula papilata Lin,1997 年(蛛形纲:Cunaxidae),首次描述该物种的雄性
IF 1.2 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-14 DOI: 10.11158/saa.29.2.6
Jian-Xin Chen, Maoyuan Yao, Yao-fang Wu, Tianci Yi, Jian-jun Guo, D. Jin
A known species, Scirula papilata Lin, 1997 based on samples collected from Hubei Province, China, is redescribed with the detailed illustration and the first description of males.
根据从中国湖北省采集的样本,对已知种 Scirula papilata Lin(1997 年)进行了重新描述,并附有详细插图和对雄性的首次描述。
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引用次数: 0
期刊
Systematic and Applied Acarology
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