The tendency to interpret ambiguous behavior of others as hostile (Hostile Attribution Bias, HAB) has been associated with aggressive behavior across clinical and non-clinical populations. Although there are several studies investigating the neural correlates of the HAB, prior reviews have not specifically focused on this aspect. This systematic review examines the existing literature on the neural correlates of the HAB and its association with aggression. PsycInfo, Embase, and PubMed databases were searched and 21 studies were included. Findings mainly from magnetic resonance imaging and electroencephalography reveal alterations in the attention/perception salience network and the theory of mind network, as well as, the attention-related P300 and the N400 component encoding expectation violation. However, the literature on this topic is very heterogeneous, inconsistent, and sparse, so conclusions about neural correlates are restricted. This review offers a comprehensive overview of neuroimaging research on HAB, underscoring the importance of incorporating adequate and standardized HAB measures in future studies. Furthermore, we discuss the potential therapeutic implications of neuroimaging findings for interventions targeting the HAB.