N. H. Aysa, Lena Fadhil Aljibouri, R. S. Almuttairi
Smart nanopolymers with the capability to release drugs on demand and in response to specific illness signals, rep¬resent a promising avenue in the field of drug delivery. Their synthesis and characterization process involves the careful design of nanopolymeric structures, incorporating stimuli-responsive elements. The responsiveness of these nanopolymers to specific illness signals is evaluated through in vitro studies that simulate physiological conditions. The potential of these nanopolymers is explored in the context of personalized medicine, where tailored drug delivery systems respond to individual patient needs. The characterization of these smart nanopolymers showcases their po¬tential as a novel and effective approach for on-demand drug release in response to illness signals. The findings contribute to the advancement of precision medicine and the development of innovative drug delivery systems with enhanced therapeutic efficacy and reduced side-effects.
{"title":"Characterization of promising smart nanopolymers that release drugs in response to illness-related signals: a review","authors":"N. H. Aysa, Lena Fadhil Aljibouri, R. S. Almuttairi","doi":"10.61873/hyis8690","DOIUrl":"https://doi.org/10.61873/hyis8690","url":null,"abstract":"Smart nanopolymers with the capability to release drugs on demand and in response to specific illness signals, rep¬resent a promising avenue in the field of drug delivery. Their synthesis and characterization process involves the careful design of nanopolymeric structures, incorporating stimuli-responsive elements. The responsiveness of these nanopolymers to specific illness signals is evaluated through in vitro studies that simulate physiological conditions. The potential of these nanopolymers is explored in the context of personalized medicine, where tailored drug delivery systems respond to individual patient needs. The characterization of these smart nanopolymers showcases their po¬tential as a novel and effective approach for on-demand drug release in response to illness signals. The findings contribute to the advancement of precision medicine and the development of innovative drug delivery systems with enhanced therapeutic efficacy and reduced side-effects.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"223 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141013027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eman Mobder Nayif, Hashim Abed Abass, Muthanna Abdulkhader Salh Al-Mahdawi, F. Abd
Stevia is herbal native to South-America that is renowned for its natural sweet leaves containing stevia glycosides. Our study aimed at examining and comparing the immunomodulatory effects of the Stevia rebaudiana leaf extract and of commercially-available stevia products in rats. Our experiment involved the preparation of Stevia rebaudiana leaf extract and the use of market-available stevia obtained from the local-market. Stevia rebaudiana leaf extraction was meticulously performed, and 60 healthy adult male rats were randomly separated into three groups: untreated control, commercial stevia treatment (25 mg/kg), and Stevia rebaudiana leaf extract treatment (25 mg/kg). The rats were orally administered the treatments for 60 days, after which blood samples were collected for analysis. Our results revealed a significant rise in interleukin-1 beta (IL-1β) levels in rats treated with the Stevia rebaudiana leaf extract and the commercially-available stevia when compared to the control group. Additionally, immunoglobulin A (IgA) levels exhibited a notable increase in both stevia-treated groups, with the Stevia rebaudiana leaf extract-receiving group showing higher IgA levels than the commercially-available stevia-treated one. Our findings suggest that stevia may influence the immune response, particularly the regulation of the pro-inflammatory cytokine IL-1β and the IgA levels. This study contributes valuable insights into stevia’s effects on the immune system.
甜叶菊是一种原产于南美洲的草本植物，以其含有甜菊糖苷的天然甜叶而闻名。我们的研究旨在研究和比较甜叶菊叶提取物和市售甜叶菊产品对大鼠的免疫调节作用。我们的实验涉及甜叶菊叶提取物的制备和从本地市场购买的市售甜叶菊的使用。我们精心提取了甜叶菊叶，并将 60 只健康的成年雄性大鼠随机分为三组：未处理对照组、商业甜叶菊处理组（25 毫克/千克）和甜叶菊叶提取物处理组（25 毫克/千克）。大鼠口服这些处理 60 天，然后采集血液样本进行分析。结果显示，与对照组相比，接受甜叶菊叶提取物和市售甜叶菊处理的大鼠体内白细胞介素-1β（IL-1β）水平明显升高。此外，两个甜菊糖处理组的免疫球蛋白 A（IgA）水平都有显著提高，其中甜叶菊叶提取物处理组的 IgA 水平高于市售甜菊糖处理组。我们的研究结果表明，甜菊糖可能会影响免疫反应，尤其是对促炎细胞因子 IL-1β 和 IgA 水平的调节。这项研究有助于深入了解甜菊糖对免疫系统的影响。
{"title":"Immunomodulatory effects of Stevia rebaudiana leaves and commercial stevia on rats: a comparative study","authors":"Eman Mobder Nayif, Hashim Abed Abass, Muthanna Abdulkhader Salh Al-Mahdawi, F. Abd","doi":"10.61873/ivjv6786","DOIUrl":"https://doi.org/10.61873/ivjv6786","url":null,"abstract":"Stevia is herbal native to South-America that is renowned for its natural sweet leaves containing stevia glycosides. Our study aimed at examining and comparing the immunomodulatory effects of the Stevia rebaudiana leaf extract and of commercially-available stevia products in rats. Our experiment involved the preparation of Stevia rebaudiana leaf extract and the use of market-available stevia obtained from the local-market. Stevia rebaudiana leaf extraction was meticulously performed, and 60 healthy adult male rats were randomly separated into three groups: untreated control, commercial stevia treatment (25 mg/kg), and Stevia rebaudiana leaf extract treatment (25 mg/kg). The rats were orally administered the treatments for 60 days, after which blood samples were collected for analysis. Our results revealed a significant rise in interleukin-1 beta (IL-1β) levels in rats treated with the Stevia rebaudiana leaf extract and the commercially-available stevia when compared to the control group. Additionally, immunoglobulin A (IgA) levels exhibited a notable increase in both stevia-treated groups, with the Stevia rebaudiana leaf extract-receiving group showing higher IgA levels than the commercially-available stevia-treated one. Our findings suggest that stevia may influence the immune response, particularly the regulation of the pro-inflammatory cytokine IL-1β and the IgA levels. This study contributes valuable insights into stevia’s effects on the immune system.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"316 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141012318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. A. Al-Hindy, Mazin J. Mousa, Muthanna Mohammed Joudah
Ceruloplasmin (CP) is an enzyme that has ferroxidase activity and is important in maintaining iron homeostasis and serving as a copper-transporter in the bloodstream. Beta-thalassemia major (BTM), a common hereditary disorder in Iraq, can affect CP activity in patients with iron overload resulting from frequent blood transfusions in order to sustain haemoglobin levels. The current study is a single-center observational research. CP activity was measured in 304 patients (120 females and 184 males). Anthropometric indices were recorded and the iron load status was determined by measuring serum ferritin. Ninety-two healthy individuals were also included as a control group. Our findings re¬vealed no relationship between age, body mass index, or plasma ferritin and CP activity in BTM patients. No significant influence of sex on CP activity was observed. The outcomes provide insight into assumed pathways regulating CP, and add to the growing body of research on CP’s contribution to iron metabolism in BTM patients. To our knowledge, this work is the only study of its kind in Iraq, provides the groundwork for upcoming studies and potential therapeutic lines by generating insightful data on the multifaceted relationships between iron homeostasis, CP, and BTM.
{"title":"Ceruloplasmin levels in β-thalassaemia major: therapeutic insights and implications for iron homeostasis","authors":"H. A. Al-Hindy, Mazin J. Mousa, Muthanna Mohammed Joudah","doi":"10.61873/sutm6297","DOIUrl":"https://doi.org/10.61873/sutm6297","url":null,"abstract":"Ceruloplasmin (CP) is an enzyme that has ferroxidase activity and is important in maintaining iron homeostasis and serving as a copper-transporter in the bloodstream. Beta-thalassemia major (BTM), a common hereditary disorder in Iraq, can affect CP activity in patients with iron overload resulting from frequent blood transfusions in order to sustain haemoglobin levels. The current study is a single-center observational research. CP activity was measured in 304 patients (120 females and 184 males). Anthropometric indices were recorded and the iron load status was determined by measuring serum ferritin. Ninety-two healthy individuals were also included as a control group. Our findings re¬vealed no relationship between age, body mass index, or plasma ferritin and CP activity in BTM patients. No significant influence of sex on CP activity was observed. The outcomes provide insight into assumed pathways regulating CP, and add to the growing body of research on CP’s contribution to iron metabolism in BTM patients. To our knowledge, this work is the only study of its kind in Iraq, provides the groundwork for upcoming studies and potential therapeutic lines by generating insightful data on the multifaceted relationships between iron homeostasis, CP, and BTM.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"275 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141012735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. F. Khalfa, Rawaa G. Abdullah, Q. Al-Daami, Aymen A. Bash, Saba Abdulmunem Habeeb Al-Qaysi, Ghassan Jasim Mohammed, Widad Abd Al-Jabbar Mozzan
A straightforward and sensitive method for high-performance liquid chromatography (HPLC) was created in order to separate and identify the di-stereoisomeric mixture of a spiro-oxalidinonic derivative of sorbinil. HPLC was carried out using a C18 reversed-phase column. The mobile phase used in the isocratic elution process was a 70:30 v/v combi¬nation of methanol and acetonitrile flowed at a rate of 2 mL/min. The procedure delivered the best result among the various attempts made. Two peaks were identified on the chromatogram, attributable to the two di-stereoisomers of the mixture analysed. Analogues of sorbinil, a powerful inhibitor of aldose reductase, have been synthesized and further tested on aldose reductase as mixtures of di-stereoisomers, exhibiting an IC50 in the order of the micromolar. The structure of the compounds was checked with nuclear magnetic resonance spectroscopy. The chemical shifts were expressed in ppm scale δ.
{"title":"Drug synthesis, separation, and identification of a di-stereoisomeric mixture of spiro-oxalidinonic derivatives of sorbinil by high-performance liquid chromatography","authors":"E. F. Khalfa, Rawaa G. Abdullah, Q. Al-Daami, Aymen A. Bash, Saba Abdulmunem Habeeb Al-Qaysi, Ghassan Jasim Mohammed, Widad Abd Al-Jabbar Mozzan","doi":"10.61873/orxr1322","DOIUrl":"https://doi.org/10.61873/orxr1322","url":null,"abstract":"A straightforward and sensitive method for high-performance liquid chromatography (HPLC) was created in order to separate and identify the di-stereoisomeric mixture of a spiro-oxalidinonic derivative of sorbinil. HPLC was carried out using a C18 reversed-phase column. The mobile phase used in the isocratic elution process was a 70:30 v/v combi¬nation of methanol and acetonitrile flowed at a rate of 2 mL/min. The procedure delivered the best result among the various attempts made. Two peaks were identified on the chromatogram, attributable to the two di-stereoisomers of the mixture analysed. Analogues of sorbinil, a powerful inhibitor of aldose reductase, have been synthesized and further tested on aldose reductase as mixtures of di-stereoisomers, exhibiting an IC50 in the order of the micromolar. The structure of the compounds was checked with nuclear magnetic resonance spectroscopy. The chemical shifts were expressed in ppm scale δ.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"54 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141011646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aftermath of monsoon rains that fell in Pakistan from June to November 2022 were catastrophic as millions of people were affected, water systems were damaged, and agricultural production was affected. The floods aggravated the already existing nutrition crisis which largely increased children’s vulnerability. Disaster Medicine and Dentistry (DMD), a UK-registered consultancy organization, provided support to the affected households in Dera Ismail Khan through the provision of water pumps, food rations, and climate-resistant houses. It also established a mobile medical unit that provided medical services to affected households. All the children that came for consultation were far below the expected height or weight range for their age, thus showing signs of chronic malnutrition. They also presented with multiorgan diseases as a result of the chronic malnutrition, and DMD’s onsite and remote consultants managed them all. We took a wholistic approach to managing the nutrition crisis and the floods, which significantly improved the health outcomes of the affected individuals and households.
{"title":"Chronic nutritional deficit resulting in multiorgan disease presentations in remote Pakistan: case reviews from natural disaster settings","authors":"Hadia Aslam","doi":"10.61873/hhhl5872","DOIUrl":"https://doi.org/10.61873/hhhl5872","url":null,"abstract":"The aftermath of monsoon rains that fell in Pakistan from June to November 2022 were catastrophic as millions of people were affected, water systems were damaged, and agricultural production was affected. The floods aggravated the already existing nutrition crisis which largely increased children’s vulnerability. Disaster Medicine and Dentistry (DMD), a UK-registered consultancy organization, provided support to the affected households in Dera Ismail Khan through the provision of water pumps, food rations, and climate-resistant houses. It also established a mobile medical unit that provided medical services to affected households. All the children that came for consultation were far below the expected height or weight range for their age, thus showing signs of chronic malnutrition. They also presented with multiorgan diseases as a result of the chronic malnutrition, and DMD’s onsite and remote consultants managed them all. We took a wholistic approach to managing the nutrition crisis and the floods, which significantly improved the health outcomes of the affected individuals and households.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"272 10‐34","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141012642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ameer Najy Obed, Thulfeqar A. Hamza, Amal Talib Al Sa’ady
COVID-19 has been a global challenge caused by a coronavirus that infects the respiratory system and poses a high risk to life. This study dealt with some immunological indicators associated with this disease. A total of 150 samples was collected from COVID-19 patients (120 samples) and non-infected individuals (30 samples; control group) who were admitted to the Marjan Medical City Hospital in Al-Hilla, Babylon, Iraq for the period from March 1 to August 30, 2022. Of these participants, 65% were men and 35% were women, with ages ranging from 16 to 75 years. The samples were collected under the supervision of specialized doctors, according to the approved by the Iraqi Ministry of Health protocols. The parameters examined in this study included the complete blood count, the red blood cell (RBC) count, the packed cell volume, the haemoglobin levels, the platelet count, the white blood cell (WBC) count, and the levels of immunological indicators such as interleukin-1 beta (IL-1β), interleukin-17 (IL-17), the tumour necro¬sis factor-alpha (TNF-α), the C-reactive protein (CRP), and the erythrocyte sedimentation rate (ESR). Our results indicate a clear increase in the WBC count, the IL-1β levels, IL-17 levels, the TNF-α levels, the CRP levels, the ESR, and the RBC count, as well as a clear decrease in the lymphocyte count, as a result of COVID-19.
{"title":"Study of some immunological indicators for COVID-19 patients in Babylon city","authors":"Ameer Najy Obed, Thulfeqar A. Hamza, Amal Talib Al Sa’ady","doi":"10.61873/getk6172","DOIUrl":"https://doi.org/10.61873/getk6172","url":null,"abstract":"COVID-19 has been a global challenge caused by a coronavirus that infects the respiratory system and poses a high risk to life. This study dealt with some immunological indicators associated with this disease. A total of 150 samples was collected from COVID-19 patients (120 samples) and non-infected individuals (30 samples; control group) who were admitted to the Marjan Medical City Hospital in Al-Hilla, Babylon, Iraq for the period from March 1 to August 30, 2022. Of these participants, 65% were men and 35% were women, with ages ranging from 16 to 75 years. The samples were collected under the supervision of specialized doctors, according to the approved by the Iraqi Ministry of Health protocols. The parameters examined in this study included the complete blood count, the red blood cell (RBC) count, the packed cell volume, the haemoglobin levels, the platelet count, the white blood cell (WBC) count, and the levels of immunological indicators such as interleukin-1 beta (IL-1β), interleukin-17 (IL-17), the tumour necro¬sis factor-alpha (TNF-α), the C-reactive protein (CRP), and the erythrocyte sedimentation rate (ESR). Our results indicate a clear increase in the WBC count, the IL-1β levels, IL-17 levels, the TNF-α levels, the CRP levels, the ESR, and the RBC count, as well as a clear decrease in the lymphocyte count, as a result of COVID-19.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"35 S1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141011393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Proper nutrition helps protect from illness and disease. Choline (Ch), an essential molecule of substantial importance for the optimal development and function of several biological systems, plays a crucial role in the pathway of one-carbon metabolism. On the other hand, Ch-deprivation (CD) has been linked with abnormal fat metabolism, insulin resistance, and myocardial dysfunction. The Ch-deficiency setting is an established experimental model of non-alco¬holic steatohepatitis that resembles the human non-alcoholic fatty liver disease (NAFLD); a disease with constantly increasing incidence and prevalence. NAFLD, commonly associated with metabolic comorbidities such as obesity and type 2 diabetes mellitus, consists a high risk for cardiovascular disease. Experimental data of dietary CD through the administration of a Ch-deficient diet to rodents have revealed myocardial monocyte infiltration along with cardiac interstitial oedema and fibrosis, as well as a deleterious effect on cardiac valves that could lead to impaired heart mechanical properties which resemble to a restrictive pattern of cardiomyopathy characterised mainly by diastolic dysfunction. In a Ch-deprived diabetic experimental model, the diastolic heart failure has been characterized by a concentric hypertrophied myocardium, a left ventricular cavity with a thinner wall, and an increased left ventricular diastolic diameter, in addition to a left atrial dilatation that could also exert functional derangement and provoke arrhythmogenesis, thereby jeopardising cardiac output.
{"title":"The interaction of choline and one-carbon / folate metabolism derangements on the cardiac remodeling process with or without diabetes","authors":"Charis Liapi","doi":"10.61873/ovue2319","DOIUrl":"https://doi.org/10.61873/ovue2319","url":null,"abstract":"Proper nutrition helps protect from illness and disease. Choline (Ch), an essential molecule of substantial importance for the optimal development and function of several biological systems, plays a crucial role in the pathway of one-carbon metabolism. On the other hand, Ch-deprivation (CD) has been linked with abnormal fat metabolism, insulin resistance, and myocardial dysfunction. The Ch-deficiency setting is an established experimental model of non-alco¬holic steatohepatitis that resembles the human non-alcoholic fatty liver disease (NAFLD); a disease with constantly increasing incidence and prevalence. NAFLD, commonly associated with metabolic comorbidities such as obesity and type 2 diabetes mellitus, consists a high risk for cardiovascular disease. Experimental data of dietary CD through the administration of a Ch-deficient diet to rodents have revealed myocardial monocyte infiltration along with cardiac interstitial oedema and fibrosis, as well as a deleterious effect on cardiac valves that could lead to impaired heart mechanical properties which resemble to a restrictive pattern of cardiomyopathy characterised mainly by diastolic dysfunction. In a Ch-deprived diabetic experimental model, the diastolic heart failure has been characterized by a concentric hypertrophied myocardium, a left ventricular cavity with a thinner wall, and an increased left ventricular diastolic diameter, in addition to a left atrial dilatation that could also exert functional derangement and provoke arrhythmogenesis, thereby jeopardising cardiac output.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"235 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141012989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Primary hepatocellular carcinoma is a devastating type of liver cancer. Silver nanoparticles (AgNPs) have been assessed for a variety of purposes, including being tested as an anticancer agent. The aim of this study was to assess the cytotoxicity of AgNPs that were biosynthesized from leech saliva on HepG2 cells, through the undertaking of a simple MTT assay. HepG2 cells were obtained from the cell bank of the Pasteur Institute of Iran. In this study, AgNP-treated HepG2 cells were cultured at a density of 104 cells per well, and 100 μL of MTT at a concentration of 0.5 mg/mL were added to each well; the treated cells were then let to incubate for 4 h. Subsequently, a plate reader device operating at a wavelength of 570 nm was used in order to determine the concentration of the chemical dissolved in isopropanol. Representative images of the cells show remarkable changes in their morphology at AgNP concentrations of 25 and 50 μg/mL. At 48 h, the nanoparticle’s IC50 value was 50 μg/mL. Our study shows that leech salivary extract-derived AgNPs are cytotoxic to HepG2 cells.
{"title":"Cytotoxic effect of silver nanoparticles biosynthesized from Hirudo medicinalis saliva on HepG2 cells","authors":"Luma Jasim Witwit","doi":"10.61873/rqjc3524","DOIUrl":"https://doi.org/10.61873/rqjc3524","url":null,"abstract":"Primary hepatocellular carcinoma is a devastating type of liver cancer. Silver nanoparticles (AgNPs) have been assessed for a variety of purposes, including being tested as an anticancer agent. The aim of this study was to assess the cytotoxicity of AgNPs that were biosynthesized from leech saliva on HepG2 cells, through the undertaking of a simple MTT assay. HepG2 cells were obtained from the cell bank of the Pasteur Institute of Iran. In this study, AgNP-treated HepG2 cells were cultured at a density of 104 cells per well, and 100 μL of MTT at a concentration of 0.5 mg/mL were added to each well; the treated cells were then let to incubate for 4 h. Subsequently, a plate reader device operating at a wavelength of 570 nm was used in order to determine the concentration of the chemical dissolved in isopropanol. Representative images of the cells show remarkable changes in their morphology at AgNP concentrations of 25 and 50 μg/mL. At 48 h, the nanoparticle’s IC50 value was 50 μg/mL. Our study shows that leech salivary extract-derived AgNPs are cytotoxic to HepG2 cells.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"306 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141012406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Network pharmacology as a “green approach”, predicting metabolite behaviours chemically and biologically and guid¬ing biological experimental design, is a new strategy aiming to uncover the mechanism of action of natural products as drug candidates. It provides a powerful way to identify novel mechanisms of natural products with potential thera¬peutic effects. This approach has emerged as a powerful tool to overcome the limitations of traditional methods, such as the ability to predict the adverse effects of a drug and the likelihood of failure during clinical trials, by applying systems biology principles to the field of pharmacology. This method combines the multi-omics dataset, computer modeling, and chemical biology so as to reveal pharmaceutical actions and guide drug discovery. Therefore, computer-aided drug design combined with network pharmacology can be viewed as a novel in silico screening ap¬proach to drug discovery, by utilising chemoinformatics, bioinformatics, structure biology, and chemical biology. This strategy includes target-based virtual screening - molecular docking, ligand similarity-based virtual screening, and inverse screening (Inver-dock), providing a powerful tool for target identification of drug candidates, multitarget dis¬covery, and natural bioactive product profiling. It can also be used for selectivity profiling of drugs, drug repositioning, safety profiling, and metabolism profiling prediction (ADMET).
{"title":"Network pharmacology speaking to ethnopharmacology: new data on an ancient remedy","authors":"Junying Liu","doi":"10.61873/zxqs4380","DOIUrl":"https://doi.org/10.61873/zxqs4380","url":null,"abstract":"Network pharmacology as a “green approach”, predicting metabolite behaviours chemically and biologically and guid¬ing biological experimental design, is a new strategy aiming to uncover the mechanism of action of natural products as drug candidates. It provides a powerful way to identify novel mechanisms of natural products with potential thera¬peutic effects. This approach has emerged as a powerful tool to overcome the limitations of traditional methods, such as the ability to predict the adverse effects of a drug and the likelihood of failure during clinical trials, by applying systems biology principles to the field of pharmacology. This method combines the multi-omics dataset, computer modeling, and chemical biology so as to reveal pharmaceutical actions and guide drug discovery. Therefore, computer-aided drug design combined with network pharmacology can be viewed as a novel in silico screening ap¬proach to drug discovery, by utilising chemoinformatics, bioinformatics, structure biology, and chemical biology. This strategy includes target-based virtual screening - molecular docking, ligand similarity-based virtual screening, and inverse screening (Inver-dock), providing a powerful tool for target identification of drug candidates, multitarget dis¬covery, and natural bioactive product profiling. It can also be used for selectivity profiling of drugs, drug repositioning, safety profiling, and metabolism profiling prediction (ADMET).","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"38 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141011548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vasculopathy is typically seen in diabetic patients, and can lead to foot ulcerations. Carbon dioxide (CO2) therapy was found to improve chronic wound healing in patients with vascular impairment. This type of therapy refers to the transcutaneous and subcutaneous application of CO2 as well as CO2 water baths for therapeutic purposes. In the method used herein, artificial CO2-containing water for foot bathing was generated by dissolving Carbothera® (MRE-SPA-MD; Mitsubishi Rayon Engineering, Tokyo, Japan) and generating CO2 (free CO2 concentration: 1,000–1,200 mg/L; pH 4.6). The foot of each patient was immersed in CO2-enriched water (depth of 20–30 cm, 37–38°C, 30-min duration) three times per week, for the next three months. Dramatic clinical improvement was observed in the CO2 water bath therapy group before and after the treatment, while both the blood levels of the vascular endothelial growth factor and of the tumour necrosis factor-alpha in these patients exhibited significant changes. The advantages of this method are the absence of pain and the protection against infection, while the improved angiogenesis and oxygena¬tion can result in healing of the chronic wound.
{"title":"Assessment of the CO2 water bath therapy effectiveness on diabetic foot ulcers through VEGF and TNF-α levels","authors":"Ghazwan Riyad Abdulhamza, Najeeb Hassan Mohammed","doi":"10.61873/onpk3289","DOIUrl":"https://doi.org/10.61873/onpk3289","url":null,"abstract":"Vasculopathy is typically seen in diabetic patients, and can lead to foot ulcerations. Carbon dioxide (CO2) therapy was found to improve chronic wound healing in patients with vascular impairment. This type of therapy refers to the transcutaneous and subcutaneous application of CO2 as well as CO2 water baths for therapeutic purposes. In the method used herein, artificial CO2-containing water for foot bathing was generated by dissolving Carbothera® (MRE-SPA-MD; Mitsubishi Rayon Engineering, Tokyo, Japan) and generating CO2 (free CO2 concentration: 1,000–1,200 mg/L; pH 4.6). The foot of each patient was immersed in CO2-enriched water (depth of 20–30 cm, 37–38°C, 30-min duration) three times per week, for the next three months. Dramatic clinical improvement was observed in the CO2 water bath therapy group before and after the treatment, while both the blood levels of the vascular endothelial growth factor and of the tumour necrosis factor-alpha in these patients exhibited significant changes. The advantages of this method are the absence of pain and the protection against infection, while the improved angiogenesis and oxygena¬tion can result in healing of the chronic wound.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"350 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141011936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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