There is a growing body of literature highlighting the potential of game-based learning (GBL) in creating immersive, highly engaging, active learning experiences. Furthermore, advances in digital technology along with the demand for online learning during the COVID-19 pandemic has brought digital GBL to the forefront of innovative teaching practice. Here, I share some of the digital GBL strategies we have developed to support our pharmacology teaching. In collab¬oration with our students, we have co-created a series of “choose your own adventure” style games using Twine; an open-source storytelling game engine. Importantly, we have shown that adoption of these GBL approaches is highly effective in promoting student engagement, subject understanding, and learning community. Furthermore, I also dis¬cuss how AI tools can allow educators with little knowledge or experience of game development to create unique and engaging learning experiences for their students.
{"title":"Technology-enhanced learning in Pharmacology through non-linear storytelling","authors":"Christina Elliott","doi":"10.61873/agtj1593","DOIUrl":"https://doi.org/10.61873/agtj1593","url":null,"abstract":"There is a growing body of literature highlighting the potential of game-based learning (GBL) in creating immersive, highly engaging, active learning experiences. Furthermore, advances in digital technology along with the demand for online learning during the COVID-19 pandemic has brought digital GBL to the forefront of innovative teaching practice. Here, I share some of the digital GBL strategies we have developed to support our pharmacology teaching. In collab¬oration with our students, we have co-created a series of “choose your own adventure” style games using Twine; an open-source storytelling game engine. Importantly, we have shown that adoption of these GBL approaches is highly effective in promoting student engagement, subject understanding, and learning community. Furthermore, I also dis¬cuss how AI tools can allow educators with little knowledge or experience of game development to create unique and engaging learning experiences for their students.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"265 33‐37","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141012800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Innovative methods and significant developments in designing new synthetic inorganic materials have been used to overcome limitations of current drug delivery systems. Inorganic polymers are widely used in the field of biomedicine, imaging, tissue engineering and drug delivery because of their bioactivity, biocompatibility, and stability. A few of the more well-known wholly inorganic polymers are portland cement, silicon dioxide, polyanionic glasses (including titania- and aluminosilicate glasses), poly(sulphur nitride), polycrystalline diamond, graphite, poly(sulphur nitride), and alumi¬num-silicate materials. Inorganic polymers, especially those possessing significant porosity, are good potential candi¬dates for the delivery of several drugs (anticancer, antibiotics, and anti-inflammatories), providing advantages such as encapsulation, controlled delivery, and improved targeting of drugs. Choosing a suitable drug carrier with a selec¬tive targeting potential also seems to be a very promising way for improving stability as well as selectivity. Despite all the advances, developing homogeneous inorganic polymers with narrow molecular weight distributions is a multidis¬ciplinary challenge. The current keynote speech provides a review of the opportunities and challenges of using inor¬ganic polymers as drug carriers.
{"title":"Inorganic polymers as drug carriers: opportunities and challenges","authors":"B. Divband","doi":"10.61873/ccdw2092","DOIUrl":"https://doi.org/10.61873/ccdw2092","url":null,"abstract":"Innovative methods and significant developments in designing new synthetic inorganic materials have been used to overcome limitations of current drug delivery systems. Inorganic polymers are widely used in the field of biomedicine, imaging, tissue engineering and drug delivery because of their bioactivity, biocompatibility, and stability. A few of the more well-known wholly inorganic polymers are portland cement, silicon dioxide, polyanionic glasses (including titania- and aluminosilicate glasses), poly(sulphur nitride), polycrystalline diamond, graphite, poly(sulphur nitride), and alumi¬num-silicate materials. Inorganic polymers, especially those possessing significant porosity, are good potential candi¬dates for the delivery of several drugs (anticancer, antibiotics, and anti-inflammatories), providing advantages such as encapsulation, controlled delivery, and improved targeting of drugs. Choosing a suitable drug carrier with a selec¬tive targeting potential also seems to be a very promising way for improving stability as well as selectivity. Despite all the advances, developing homogeneous inorganic polymers with narrow molecular weight distributions is a multidis¬ciplinary challenge. The current keynote speech provides a review of the opportunities and challenges of using inor¬ganic polymers as drug carriers.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"273 29‐32","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141012941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Jalil Al-Saigh, Ihab Saeed Ahmed, Sally Saad Bash, Muataz Fawzi Hussein, Mohamed AbdElrahman, Rafal J. Al-Saigh
Acute decompensated heart failure (ADHF) is a leading cause of hospital admission and many factors are known to precipitate decompensation. We aimed to assess the decompensating factors of heart failure and the management of patients admitted to the emergency department (ED). A total of 107 patients were examined, all diagnosed with ADHF in the ED of the Baghdad Teaching Hospital, from June 2017 to December 2017, and presenting with decom¬pensation (pulmonary oedema, peripheral oedema, and fatigue). The mean patient age was 62.5 ± 9.8 years (range: 43–85 years); the majority of them were in their 7th decade (37.4%), and men were slightly more than women. Hy¬pertension was the most commonly associated comorbidity (68.2%), followed by diabetes mellitus (57.9%), coronary artery disease (51.4%), dyslipidaemia (37.4%), arrhythmia (28%), and chronic obstructive pulmonary disease / asthma (23.4%). The most common presentation was pulmonary oedema (88.8%) followed by peripheral oedema (61.7%), and fatigue (26.2%). Uncontrolled hypertension was the most common precipitating condition for decompen¬sation (58.9%), followed by infection (39.3%), acute coronary syndrome (31.8%), arrhythmia (27.1%), non-compliance (11.2%), and anaemia (2.8%). The majority of the admitted patients were managed with intravenously-administered (i.v.) diuretics (92.5%) that may have been combined with oxygen therapy (63.6%), antibiotics (58.9%), β-blockers (50.5%), nitroglycerin (40.2%), i.v. fluids (38.3%), and/or digoxin (19.6%).
{"title":"Emergency department presentation and management of patients with acute decompensated heart failure at the Baghdad Teaching Hospital","authors":"Ali Jalil Al-Saigh, Ihab Saeed Ahmed, Sally Saad Bash, Muataz Fawzi Hussein, Mohamed AbdElrahman, Rafal J. Al-Saigh","doi":"10.61873/rygm9424","DOIUrl":"https://doi.org/10.61873/rygm9424","url":null,"abstract":"Acute decompensated heart failure (ADHF) is a leading cause of hospital admission and many factors are known to precipitate decompensation. We aimed to assess the decompensating factors of heart failure and the management of patients admitted to the emergency department (ED). A total of 107 patients were examined, all diagnosed with ADHF in the ED of the Baghdad Teaching Hospital, from June 2017 to December 2017, and presenting with decom¬pensation (pulmonary oedema, peripheral oedema, and fatigue). The mean patient age was 62.5 ± 9.8 years (range: 43–85 years); the majority of them were in their 7th decade (37.4%), and men were slightly more than women. Hy¬pertension was the most commonly associated comorbidity (68.2%), followed by diabetes mellitus (57.9%), coronary artery disease (51.4%), dyslipidaemia (37.4%), arrhythmia (28%), and chronic obstructive pulmonary disease / asthma (23.4%). The most common presentation was pulmonary oedema (88.8%) followed by peripheral oedema (61.7%), and fatigue (26.2%). Uncontrolled hypertension was the most common precipitating condition for decompen¬sation (58.9%), followed by infection (39.3%), acute coronary syndrome (31.8%), arrhythmia (27.1%), non-compliance (11.2%), and anaemia (2.8%). The majority of the admitted patients were managed with intravenously-administered (i.v.) diuretics (92.5%) that may have been combined with oxygen therapy (63.6%), antibiotics (58.9%), β-blockers (50.5%), nitroglycerin (40.2%), i.v. fluids (38.3%), and/or digoxin (19.6%).","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"270 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141012660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alaa Hamady Obeid Al-Taei, R. Saleh, S. Kadhum, A. Omran, R. M. Ewadh, H. H. Owadh
The antimicrobial activity of the aqueous and ethanolic extracts of Myrtus communis, Ammi visnaga, and Equisetum arvense was investigated against Staphylococcus aureus, Escherichia coli, and Candida albicans by using the agar well diffusion method. Serial concentrations (15%, 30%, and 50%) of the extracts of each plant were tested and compared with gentamicin (10 μg) and fluconazole (25 μg). Most of the extract concentrations showed a relatively high antimicrobial activity against all the tested microbes, and the ethanolic extract was more effective than the aque¬ous extract. The activity of plant extracts increased with the increasing extract concentration of Myrtus communis, which appeared to possess a more antimicrobial activity than the other plants assessed; in fact, its ethanolic extract exhibited the highest inhibition zone against S. aureus (32 mm). The ethanolic plant extracts at a concentration of 50% displayed the maximum activity against the herein assessed isolates. Moreover, E. coli showed a higher sensi¬tivity to most extracts, while the lowest effect being noticed on C. albicans.
{"title":"Phytochemical screening and antimicrobial activity of some medicinal plants","authors":"Alaa Hamady Obeid Al-Taei, R. Saleh, S. Kadhum, A. Omran, R. M. Ewadh, H. H. Owadh","doi":"10.61873/txet1445","DOIUrl":"https://doi.org/10.61873/txet1445","url":null,"abstract":"The antimicrobial activity of the aqueous and ethanolic extracts of Myrtus communis, Ammi visnaga, and Equisetum arvense was investigated against Staphylococcus aureus, Escherichia coli, and Candida albicans by using the agar well diffusion method. Serial concentrations (15%, 30%, and 50%) of the extracts of each plant were tested and compared with gentamicin (10 μg) and fluconazole (25 μg). Most of the extract concentrations showed a relatively high antimicrobial activity against all the tested microbes, and the ethanolic extract was more effective than the aque¬ous extract. The activity of plant extracts increased with the increasing extract concentration of Myrtus communis, which appeared to possess a more antimicrobial activity than the other plants assessed; in fact, its ethanolic extract exhibited the highest inhibition zone against S. aureus (32 mm). The ethanolic plant extracts at a concentration of 50% displayed the maximum activity against the herein assessed isolates. Moreover, E. coli showed a higher sensi¬tivity to most extracts, while the lowest effect being noticed on C. albicans.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"340 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141011972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mazin J. Mousa, H. A. Al-Hindy, Amir Saheb Al-Mumin
Cystatin C (CysC) levels in patients with multiple myeloma (MM) have been linked to tumour load and outcomes’ prediction. This study aimed at assessing the diagnostic utility of CysC in distinguishing MM patients from controls and advanced stages of MM. In total, 98 MM patients and 57 healthy controls participated in this cross-sectional case-control study. Demographic, clinical, and biochemical data were assessed. The study groups exhibited significantly diverse measures of urea, creatinine, CysC, β2-microglobulin, and lactate dehydrogenase activity. β2-Microglobulin was found to be a reliable predictor for both the MM staging and its diagnosis, but CysC was found to only possess a partial capacity of predicting advanced MM stages. Our results highlight the significance of taking into account many biomarkers in the therapy of MM, so as to achieve effective clinical evaluation. More research is required in order to clarify the CysC implication in the prognosis and management of MM.
多发性骨髓瘤（MM）患者的胱抑素C（CysC）水平与肿瘤负荷和预后有关。本研究旨在评估胱抑素C在区分多发性骨髓瘤患者和对照组以及晚期多发性骨髓瘤方面的诊断作用。共有98名MM患者和57名健康对照者参加了这项横断面病例对照研究。研究人员评估了人口统计学、临床和生化数据。研究组在尿素、肌酐、CysC、β2-微球蛋白和乳酸脱氢酶活性方面表现出明显的差异。研究发现，β2-微球蛋白是预测 MM 分期及其诊断的可靠指标，但 CysC 仅具有预测 MM 晚期的部分能力。我们的研究结果凸显了在治疗 MM 时考虑多种生物标志物以实现有效临床评估的重要性。为了明确CysC在MM预后和治疗中的作用，还需要更多的研究。
{"title":"Evaluation of serum cystatin C levels in multiple myeloma: diagnostic significance and clinical implications","authors":"Mazin J. Mousa, H. A. Al-Hindy, Amir Saheb Al-Mumin","doi":"10.61873/hhpt2430","DOIUrl":"https://doi.org/10.61873/hhpt2430","url":null,"abstract":"Cystatin C (CysC) levels in patients with multiple myeloma (MM) have been linked to tumour load and outcomes’ prediction. This study aimed at assessing the diagnostic utility of CysC in distinguishing MM patients from controls and advanced stages of MM. In total, 98 MM patients and 57 healthy controls participated in this cross-sectional case-control study. Demographic, clinical, and biochemical data were assessed. The study groups exhibited significantly diverse measures of urea, creatinine, CysC, β2-microglobulin, and lactate dehydrogenase activity. β2-Microglobulin was found to be a reliable predictor for both the MM staging and its diagnosis, but CysC was found to only possess a partial capacity of predicting advanced MM stages. Our results highlight the significance of taking into account many biomarkers in the therapy of MM, so as to achieve effective clinical evaluation. More research is required in order to clarify the CysC implication in the prognosis and management of MM.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141011895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. M. Al-Kawaz, Hussein Jasim Alharbi, Fakhir Magtoof Al-Zubaidy
Gastric ulcer is the most common health concern due to alcohol consumption, smoking, and physiological stress. An ethanol-induced gastric ulcer in an animal model resembles the pathophysiology of the human ulcer. The present study attempted to detect the protective effects of esomeprazole, curcumin, chitosan, and a mixture of curcumin and chitosan on ethanol-induced gastric ulcers in female rats. The present study included 60 rats with an average weight between 179.1 and 180.3 g, divided into two control groups and four treated groups (esomeprazole, curcumin, chi¬tosan, and mixture), where each group included 10 rats. All groups were treated for 30 days. In order to induce a gastric ulcer, absolute ethanol (2 mL/rat) was given orally to all groups (except the negative control ones) after a period of fasting of 20 h. All animals were sacrificed 5 h later. The gastric ulceration was studied by comparing the volume and the pH of the gastric juice, the ulcer index as well as the protective index. Our results revealed a significant decrease (P<0.05) in the values of the ulcer index and the volume of gastric juice in the esomeprazole-, curcumin-, chitosan-, and mixture-treated rats as compared to those of the positive control group. The value of the gastric juice pH exhibited a significant increase (P<0.05) in these same groups.
{"title":"Protective effects of esomeprazole, curcumin, chitosan, and curcumin-chitosan mixture on ethanol-induced gastric mucosal injuries in female rats","authors":"J. M. Al-Kawaz, Hussein Jasim Alharbi, Fakhir Magtoof Al-Zubaidy","doi":"10.61873/danb7861","DOIUrl":"https://doi.org/10.61873/danb7861","url":null,"abstract":"Gastric ulcer is the most common health concern due to alcohol consumption, smoking, and physiological stress. An ethanol-induced gastric ulcer in an animal model resembles the pathophysiology of the human ulcer. The present study attempted to detect the protective effects of esomeprazole, curcumin, chitosan, and a mixture of curcumin and chitosan on ethanol-induced gastric ulcers in female rats. The present study included 60 rats with an average weight between 179.1 and 180.3 g, divided into two control groups and four treated groups (esomeprazole, curcumin, chi¬tosan, and mixture), where each group included 10 rats. All groups were treated for 30 days. In order to induce a gastric ulcer, absolute ethanol (2 mL/rat) was given orally to all groups (except the negative control ones) after a period of fasting of 20 h. All animals were sacrificed 5 h later. The gastric ulceration was studied by comparing the volume and the pH of the gastric juice, the ulcer index as well as the protective index. Our results revealed a significant decrease (P<0.05) in the values of the ulcer index and the volume of gastric juice in the esomeprazole-, curcumin-, chitosan-, and mixture-treated rats as compared to those of the positive control group. The value of the gastric juice pH exhibited a significant increase (P<0.05) in these same groups.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"352 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141011930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Azelnidipine is a calcium-channel antagonist classified as a “class 2” drug with high permeability and low aqueous solubility. It is used in the treatment of angina pectoris and hypertension without reflex tachycardia. Improvement of the solubility of azelnidipine and increasing drug’s bioavailability can be achieved through the drug encapsulation in solutol / D-α-tocopheryl polyethylene glycol succinate (TPGS) micelles. Six formulas were prepared by direct disso¬lution after using different amounts of solutol and TPGS. TPGS and solutol act as solubilizers, permeation enhancers, and P-glycoprotein inhibitors. The particle size, particle size distribution, zeta potential, and entrapment efficiency were determined. Depending on particle size and entrapment efficiency, formula #6 was selected and subjected to in vitro dilution stability and in vitro release studies. The results obtained showed that formula #6 was the best formula, with a high entrapment efficiency percentage equal to 86.5%±0.58% and a small particle size equal to 21.9±7.75 nm that did not change significantly after dilution up to 100-fold; a fact that reveals the high thermodynamic and kinetic stability of the optimum formula. The formula #6 release profile showed a controlled release of the drug from micelles when compared to plain drug release. Based on these results, polymeric nanomicelles are regarded as a promising delivery system for azelnidipine.
{"title":"Preparation and characterization of azelnidipine-loaded D-α-tocopheryl polyethylene glycol succinate (TPGS) / solutol micelles","authors":"Ali Kathem Ala Allah, Shaimaa Nazar Abd Alhammid","doi":"10.61873/jyhn9753","DOIUrl":"https://doi.org/10.61873/jyhn9753","url":null,"abstract":"Azelnidipine is a calcium-channel antagonist classified as a “class 2” drug with high permeability and low aqueous solubility. It is used in the treatment of angina pectoris and hypertension without reflex tachycardia. Improvement of the solubility of azelnidipine and increasing drug’s bioavailability can be achieved through the drug encapsulation in solutol / D-α-tocopheryl polyethylene glycol succinate (TPGS) micelles. Six formulas were prepared by direct disso¬lution after using different amounts of solutol and TPGS. TPGS and solutol act as solubilizers, permeation enhancers, and P-glycoprotein inhibitors. The particle size, particle size distribution, zeta potential, and entrapment efficiency were determined. Depending on particle size and entrapment efficiency, formula #6 was selected and subjected to in vitro dilution stability and in vitro release studies. The results obtained showed that formula #6 was the best formula, with a high entrapment efficiency percentage equal to 86.5%±0.58% and a small particle size equal to 21.9±7.75 nm that did not change significantly after dilution up to 100-fold; a fact that reveals the high thermodynamic and kinetic stability of the optimum formula. The formula #6 release profile showed a controlled release of the drug from micelles when compared to plain drug release. Based on these results, polymeric nanomicelles are regarded as a promising delivery system for azelnidipine.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"262 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141012958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Regulatory B-cells (Breg cells) represent as an important modulator of the immune system and their role is unique in autoimmunity, infection, tolerance to transplants, allergy, and cancer. Several regulatory mechanisms exist by which Breg cells can control the function of other immune cells through two main pathways: the secretion of soluble mole¬cules and the use of cell surface-expressed molecules. Anti-inflammatory cytokine interleukin-10 acts as the hallmark of Breg cell function; other cytokines with a similar role include the transforming growth factor-beta and interleukin-35. Breg B cells also release the cytotoxic granzyme B that mediates cell apoptosis. Cell surface-expressed proteins include FasL, CD80, CD86, CD73, CD1d, and PD-L1. The present article reviews the immunosuppressive pathways in order to understand how they emerge and are induced to evoke their regulatory activities, and how we can benefit from them in the field of immunotherapy.
调节性 B 细胞（Breg 细胞）是免疫系统的重要调节因子，在自身免疫、感染、移植耐受、过敏和癌症中发挥着独特的作用。Breg细胞可通过两种主要途径控制其他免疫细胞的功能：分泌可溶性分子和利用细胞表面表达的分子。抗炎细胞因子白细胞介素-10是Breg细胞功能的标志；其他具有类似作用的细胞因子包括转化生长因子-β和白细胞介素-35。Breg B 细胞还会释放细胞毒性颗粒酶 B，介导细胞凋亡。细胞表面表达的蛋白包括 FasL、CD80、CD86、CD73、CD1d 和 PD-L1。本文回顾了这些免疫抑制途径，以了解它们是如何出现和被诱导以唤起其调节活动的，以及我们如何在免疫疗法领域从中获益。
{"title":"Regulatory B-cells: immunomodulating mechanisms and important cellular targets underlining immunotherapy by immunoregulation","authors":"Zaman I. L. Al-Kaabi","doi":"10.61873/baow5445","DOIUrl":"https://doi.org/10.61873/baow5445","url":null,"abstract":"Regulatory B-cells (Breg cells) represent as an important modulator of the immune system and their role is unique in autoimmunity, infection, tolerance to transplants, allergy, and cancer. Several regulatory mechanisms exist by which Breg cells can control the function of other immune cells through two main pathways: the secretion of soluble mole¬cules and the use of cell surface-expressed molecules. Anti-inflammatory cytokine interleukin-10 acts as the hallmark of Breg cell function; other cytokines with a similar role include the transforming growth factor-beta and interleukin-35. Breg B cells also release the cytotoxic granzyme B that mediates cell apoptosis. Cell surface-expressed proteins include FasL, CD80, CD86, CD73, CD1d, and PD-L1. The present article reviews the immunosuppressive pathways in order to understand how they emerge and are induced to evoke their regulatory activities, and how we can benefit from them in the field of immunotherapy.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"21 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141011730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hutham Abdulilah Amer Aladeli, A. Althahab, S. Jabuk
A total of 164 food samples were collected from various supermarkets and farmers in various areas of Babylon, were placed in plastic containers, and were transported to the laboratory. The isolation of Bacillus species was performed by culture in blood agar, chromogenic agar, and the absence of growth on McConkey agar, by staining with Gram stain, and through molecular identification of the species’ 16SrRNA gene and sequencing. The antibiotic susceptibility test used eight types of antibiotics. The undertaken sequencing identified the Bacillus isolates to belong to the follow¬ing species: B. subtilis (2), B. cereus (6), B. thuringiensis (1), B. anthracis (1), and B. spizizenii (1). The susceptibility test of the six B. cereus isolates revealed that 5 (83.3%), 4 (66.6%), 3 (50%), 2 (33.3%), 2 (33.3%), 2 (33.3%), 1 (16.6%), and 0 (0%) were resistant to rifampicin, clindamycin, erythromycin, tetracycline, trimethoprim, nitrofurantoin, gentamicin, and ciprofloxacin, while the respective resistance numbers for the two isolates of B. subtilis were 2 (100%), 2 (100%), 2 (100%), 0 (0%), 1 (50%), 0 (0%), 0 (0%), and 0 (0%). One isolate of B. thuringiensis presented resistance to erythromycin, erythromycin, trimethoprim, and rifampicin, while one isolate of B. anthracis was found to be resistant to gentamicin, erythromycin, nitrofurantoin, and rifampicin. The B. spizizenii isolate was resistant to all antibiotics except gentamicin and trimethoprim.
{"title":"Antibiotic resistance of Bacillus species isolated in foodstuff samples that were collected in Babylon (Iraq)","authors":"Hutham Abdulilah Amer Aladeli, A. Althahab, S. Jabuk","doi":"10.61873/xrsq1725","DOIUrl":"https://doi.org/10.61873/xrsq1725","url":null,"abstract":"A total of 164 food samples were collected from various supermarkets and farmers in various areas of Babylon, were placed in plastic containers, and were transported to the laboratory. The isolation of Bacillus species was performed by culture in blood agar, chromogenic agar, and the absence of growth on McConkey agar, by staining with Gram stain, and through molecular identification of the species’ 16SrRNA gene and sequencing. The antibiotic susceptibility test used eight types of antibiotics. The undertaken sequencing identified the Bacillus isolates to belong to the follow¬ing species: B. subtilis (2), B. cereus (6), B. thuringiensis (1), B. anthracis (1), and B. spizizenii (1). The susceptibility test of the six B. cereus isolates revealed that 5 (83.3%), 4 (66.6%), 3 (50%), 2 (33.3%), 2 (33.3%), 2 (33.3%), 1 (16.6%), and 0 (0%) were resistant to rifampicin, clindamycin, erythromycin, tetracycline, trimethoprim, nitrofurantoin, gentamicin, and ciprofloxacin, while the respective resistance numbers for the two isolates of B. subtilis were 2 (100%), 2 (100%), 2 (100%), 0 (0%), 1 (50%), 0 (0%), 0 (0%), and 0 (0%). One isolate of B. thuringiensis presented resistance to erythromycin, erythromycin, trimethoprim, and rifampicin, while one isolate of B. anthracis was found to be resistant to gentamicin, erythromycin, nitrofurantoin, and rifampicin. The B. spizizenii isolate was resistant to all antibiotics except gentamicin and trimethoprim.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"322 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141012028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Due to their unique physical and chemical properties, the potential application of nanomaterials in medicine is partic¬ularly attractive. Despite the many advantages that nanomaterials can offer as diagnostic and therapeutic tools, their transition from the bench to clinical practice is extremely challenging. One of the many barriers that nanomedicines may encounter is their toxicological effect. In fact, the development of novel nanomaterials / nanoparticles must pro¬ceed always in tandem with the assessment of any potential toxicological effects associated to them. Once nano¬materials reach the systemic circulation, they interact with endothelial cells, plasma proteins, and other blood compo¬nents. There is no doubt that the study of nanomaterials-blood interactions is crucial to warrant the biocompatibility of nanomaterials developed for human use.
{"title":"Applications of quartz crystal microbalance with dissipation in nanomedicine (QCM-D): a personal experience","authors":"M. Santos-Martinez","doi":"10.61873/fkiq6626","DOIUrl":"https://doi.org/10.61873/fkiq6626","url":null,"abstract":"Due to their unique physical and chemical properties, the potential application of nanomaterials in medicine is partic¬ularly attractive. Despite the many advantages that nanomaterials can offer as diagnostic and therapeutic tools, their transition from the bench to clinical practice is extremely challenging. One of the many barriers that nanomedicines may encounter is their toxicological effect. In fact, the development of novel nanomaterials / nanoparticles must pro¬ceed always in tandem with the assessment of any potential toxicological effects associated to them. Once nano¬materials reach the systemic circulation, they interact with endothelial cells, plasma proteins, and other blood compo¬nents. There is no doubt that the study of nanomaterials-blood interactions is crucial to warrant the biocompatibility of nanomaterials developed for human use.","PeriodicalId":515365,"journal":{"name":"Review of Clinical Pharmacology and Pharmacokinetics - International Edition","volume":"23 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141012031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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