Pub Date : 2020-07-21DOI: 10.46300/91011.2020.14.15
E. A. Kolesnik, M. Derkho, V. Strizhikov, S. Strizhikova, F. Gizatullina, T. Ponomaryova
In accordance with the recommendations of The International Council for Standardization in Haematology (ICSH, https://icsh.org/), this article describes the morphophysiological characteristics of the precursors and mature erythroid cells in the early period of postnatal development of birds (Gallus gallus L.) including calculation of the surface area of these cells (S, μm2 , X±SEM). Depending on cell shape, cytoplasm color, and chromatin organization in nucleus, the following types are distinguished: basophilic erythroblasts (69.60±4.01 μm2 , p≤0.05), polychromatophilic erythroblasts (65.42±2.49 μm2 , p≤0.05), and oxyphilic erythroblasts (71.10±4.43 μm2). Formation of cell pool is characteristic for erythropoiesis in birds due to mitotic proliferation of basophilic erythroblasts. There are often proerythroblasts and polychromatophilic erythroblasts. The nucleus of a polychromatophilic proerythroblast contains a large number of histone proteins; therefore, it has an intensely basophilic color with a pronounced oxyphilic hue (proteinrelated oxyphilia). The accumulation of hemoglobin in the protoplasm of these cells contributes to the gradual transition of the basophilic staining of cytoplasm to the oxyphilic one which is typical for mature red blood cells (73.95±2.10 μm2 , p≤0.05). Cell shape and the structure of erythroblast nucleus approaches to these of mature red blood cells.
{"title":"Differential Morphophysiological Characteristics of Erythrocyte Precursors and Mature Erythroid Cells in Early Postnatal Ontogenesis of Birds","authors":"E. A. Kolesnik, M. Derkho, V. Strizhikov, S. Strizhikova, F. Gizatullina, T. Ponomaryova","doi":"10.46300/91011.2020.14.15","DOIUrl":"https://doi.org/10.46300/91011.2020.14.15","url":null,"abstract":"In accordance with the recommendations of The International Council for Standardization in Haematology (ICSH, https://icsh.org/), this article describes the morphophysiological characteristics of the precursors and mature erythroid cells in the early period of postnatal development of birds (Gallus gallus L.) including calculation of the surface area of these cells (S, μm2 , X±SEM). Depending on cell shape, cytoplasm color, and chromatin organization in nucleus, the following types are distinguished: basophilic erythroblasts (69.60±4.01 μm2 , p≤0.05), polychromatophilic erythroblasts (65.42±2.49 μm2 , p≤0.05), and oxyphilic erythroblasts (71.10±4.43 μm2). Formation of cell pool is characteristic for erythropoiesis in birds due to mitotic proliferation of basophilic erythroblasts. There are often proerythroblasts and polychromatophilic erythroblasts. The nucleus of a polychromatophilic proerythroblast contains a large number of histone proteins; therefore, it has an intensely basophilic color with a pronounced oxyphilic hue (proteinrelated oxyphilia). The accumulation of hemoglobin in the protoplasm of these cells contributes to the gradual transition of the basophilic staining of cytoplasm to the oxyphilic one which is typical for mature red blood cells (73.95±2.10 μm2 , p≤0.05). Cell shape and the structure of erythroblast nucleus approaches to these of mature red blood cells.","PeriodicalId":53488,"journal":{"name":"International Journal of Biology and Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48460617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-21DOI: 10.46300/91011.2020.14.14
G. Sebastiani, Stig Uteng, F. Godtliebsen, J. Polák, J. Brož
In this paper, we describe a new statistical approach to estimate blood glucose concentration along time during endurance sports based on measurements of glucose concentration in subcutaneous interstitial tissue. The final goal is the monitoring of glucose concentration in blood to maximize performance in endurance sports. Blood glucose concentration control during and after aerobic physical activity could also be useful to reduce the risk of hypoglycemia in type 1 diabetes mellitus subjects. By means of a low invasive technology known as "continuous glucose monitoring", glucose concentration in subcutaneous interstitial tissue can now be measured every five minutes. However, it can be expressed as function of blood glucose concentration along time by means of a convolution integral equation. In the training phase of the proposed approach, based on measurements of glucose concentration in both artery and subcutaneous interstitial tissue during physical activity, the parameters of the convolution kernel are estimated. Then, given a new subject performing aerobic physical activity, a deconvolution problem is solved to estimate glucose concentration in blood from continuous glucose monitoring measurements
{"title":"Estimation of Blood Glucose Concentration During Endurance Sports","authors":"G. Sebastiani, Stig Uteng, F. Godtliebsen, J. Polák, J. Brož","doi":"10.46300/91011.2020.14.14","DOIUrl":"https://doi.org/10.46300/91011.2020.14.14","url":null,"abstract":"In this paper, we describe a new statistical approach to estimate blood glucose concentration along time during endurance sports based on measurements of glucose concentration in subcutaneous interstitial tissue. The final goal is the monitoring of glucose concentration in blood to maximize performance in endurance sports. Blood glucose concentration control during and after aerobic physical activity could also be useful to reduce the risk of hypoglycemia in type 1 diabetes mellitus subjects. By means of a low invasive technology known as \"continuous glucose monitoring\", glucose concentration in subcutaneous interstitial tissue can now be measured every five minutes. However, it can be expressed as function of blood glucose concentration along time by means of a convolution integral equation. In the training phase of the proposed approach, based on measurements of glucose concentration in both artery and subcutaneous interstitial tissue during physical activity, the parameters of the convolution kernel are estimated. Then, given a new subject performing aerobic physical activity, a deconvolution problem is solved to estimate glucose concentration in blood from continuous glucose monitoring measurements","PeriodicalId":53488,"journal":{"name":"International Journal of Biology and Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45483090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-20DOI: 10.46300/91011.2020.14.12
Recent developments in drug discovery have highlighted the ability of hydroxamic acids to form complexes with various metal ions, in particular iron, zinc, magnesium and calcium, and this imparts them with a number of unique biological and pharmacological properties. This review provides information on the most significant developments of the hydroxamate compounds in the medicinal area with a focus on Suberoylanilide hydroxamic acid (SAHA) and its derivatives with antibacterial, antifungal, antiviral, antimalarial, antitubercular and anticancer effect and their possible molecular mechanisms. One of the latest favorable developments has been on developing SAHA and its hybrids as potent anti-tuberculosis drugs. Moreover, the combination of the hydroxamic acids with other pharmacophore groups in one molecule can lead to the creation of a huge number of new compounds with promising properties as a multifunctional drug. Also, within the last decade, active research has focused to create these compounds into a class of new antiviral drugs designed to treat influenza, hepatitis C, and other viral infections. Thus, both linear hydroxamic acids and cyclic acids having the uniqueness of the set of bioactive properties, have been compiled here and could be looked forward as the basis for the development of effective drugs and forpilot scale productions through nitrile and amides via chemical and biological transformations using amidase and acyltransferase enzymes. Such synthesis of medicinally significant compounds from amide containing waste derived feedstocks using microbial agents is also a key factor for stimulating bioeconomy.
{"title":"A review on Hydroxamic Acids: Widespectrum Chemotherapeutic Agents","authors":"","doi":"10.46300/91011.2020.14.12","DOIUrl":"https://doi.org/10.46300/91011.2020.14.12","url":null,"abstract":"Recent developments in drug discovery have highlighted the ability of hydroxamic acids to form complexes with various metal ions, in particular iron, zinc, magnesium and calcium, and this imparts them with a number of unique biological and pharmacological properties. This review provides information on the most significant developments of the hydroxamate compounds in the medicinal area with a focus on Suberoylanilide hydroxamic acid (SAHA) and its derivatives with antibacterial, antifungal, antiviral, antimalarial, antitubercular and anticancer effect and their possible molecular mechanisms. One of the latest favorable developments has been on developing SAHA and its hybrids as potent anti-tuberculosis drugs. Moreover, the combination of the hydroxamic acids with other pharmacophore groups in one molecule can lead to the creation of a huge number of new compounds with promising properties as a multifunctional drug. Also, within the last decade, active research has focused to create these compounds into a class of new antiviral drugs designed to treat influenza, hepatitis C, and other viral infections. Thus, both linear hydroxamic acids and cyclic acids having the uniqueness of the set of bioactive properties, have been compiled here and could be looked forward as the basis for the development of effective drugs and forpilot scale productions through nitrile and amides via chemical and biological transformations using amidase and acyltransferase enzymes. Such synthesis of medicinally significant compounds from amide containing waste derived feedstocks using microbial agents is also a key factor for stimulating bioeconomy.","PeriodicalId":53488,"journal":{"name":"International Journal of Biology and Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42116328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-02DOI: 10.46300/91011.2020.14.11
A. Aliev, B. B. Bulathanov, A. Barkova
The aim of the vark was to study of changes in the structure of the mammary gland in vork after treatment of catarrhal mastitis by preparation dioxinor with combined use of oxytocin and novocain blockade according to D.D. Logvinov. The work was carried out in SEC "Gasan" of Dagestan Republic on the ewes of Dagestan rock, in number of 37 heads with catarrhal mastitis. Three sheep for study of pathoanatomical and histological studies were scored before treatment, and three-after treatment. In sik ewes, a thickening of the mucous membrane of the cistern and large ducts was noted macroscopically, in places with spot-spot hemorrhages, histological studies established dystrophy and necrobiosis of lactocytes, which was accompanied by active desquamation. In the treatment of catarrhal mastitis with a drug dioxinor in combination with oxytocin and novocaine blockade on the seventh day, with a pathoanatomical study, the structure of the mammary gland was normal, and histological examination revealed the formation of a stable recovery process in the mammary gland, manifested in the purification of the alveoli from the exudate, the restoration of the epithelium.
{"title":"Effective Method of Therapy of Catarrhal Mastitis of Sheep","authors":"A. Aliev, B. B. Bulathanov, A. Barkova","doi":"10.46300/91011.2020.14.11","DOIUrl":"https://doi.org/10.46300/91011.2020.14.11","url":null,"abstract":"The aim of the vark was to study of changes in the structure of the mammary gland in vork after treatment of catarrhal mastitis by preparation dioxinor with combined use of oxytocin and novocain blockade according to D.D. Logvinov. The work was carried out in SEC \"Gasan\" of Dagestan Republic on the ewes of Dagestan rock, in number of 37 heads with catarrhal mastitis. Three sheep for study of pathoanatomical and histological studies were scored before treatment, and three-after treatment. In sik ewes, a thickening of the mucous membrane of the cistern and large ducts was noted macroscopically, in places with spot-spot hemorrhages, histological studies established dystrophy and necrobiosis of lactocytes, which was accompanied by active desquamation. In the treatment of catarrhal mastitis with a drug dioxinor in combination with oxytocin and novocaine blockade on the seventh day, with a pathoanatomical study, the structure of the mammary gland was normal, and histological examination revealed the formation of a stable recovery process in the mammary gland, manifested in the purification of the alveoli from the exudate, the restoration of the epithelium.","PeriodicalId":53488,"journal":{"name":"International Journal of Biology and Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44963483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-14DOI: 10.46300/91011.2020.14.9
A. Panfilov, N. N. Zezin, N. Kazakova, M. A. Namyatov
Middle and Southern Urals’ maize growing area is characterised by a wide range of edaphoclimatic conditions and by a variety of factors which may limit growth and development of maize. The factors may vary from severe warmth shortage in the North of the region to sharp aridity in the South. Consequently, fast-ripening maize breeding is dictated by limited heat resources. Depending on the growing area and the purpose of the crops (silage or corn), hybrids adapted for the regions must be characterised within the limits of 110 and 170 according to FAO classification. The purpose of the research is to measure ultra-early maize hybrids’ adaptability within forest-meadow and forest steppe zones of the Middle and the South Urals on maize’s growth stability and ripening, crop productivity and grain moisture at harvest. During the period from 1999 to 2019 experimental hybrid combinations and FAO 100-120 type industrial hybrids were assessed. These hybrids were created with the use of a local maize variety of Northern Caucasia, West Siberia and foothill areas of the Republic of Altay as a source material. Local maize varieties of Northern Caucasia, West Siberia and foothill areas of the Republic of Altay were used as initial material to create these hybrids. As a result of these three research phases early blossom maize varieties’ competitive advantages were discovered. These varieties’ grain formation and grain filling last within a favourable temperature background and have their biological ripeness before diurnal temperature achieves biological minimum required for maize growth. This advantage ensures minimum grain moisture at harvest and maximum genetic potentiality’s productivity implementation thanks to grain filling of full value. This advantage also assures high starch content and highly digestible energy concentration in dry matter. It has been established that ultra-early hybrids’ cultivation is the main condition to obtain high energy fodder in northern areas of the Urals despite of minor gross productivity loss. Commercial maize hybrids F1 Koubanskii 101 SV and Koubanskii 102 MV pass development stages from sprouts to ear corn blossom within the limits of 44 to 58 days depending on the vegetation period conditions. These hybrids’ cultivation in forest-steppe areas is of importance to produce dried and canned corn. Forest-meadow cultivation is of importance to obtain high grain canned products and high energy silage
{"title":"Adaptive Approach in Maize Breeding for the Urals Region","authors":"A. Panfilov, N. N. Zezin, N. Kazakova, M. A. Namyatov","doi":"10.46300/91011.2020.14.9","DOIUrl":"https://doi.org/10.46300/91011.2020.14.9","url":null,"abstract":"Middle and Southern Urals’ maize growing area is characterised by a wide range of edaphoclimatic conditions and by a variety of factors which may limit growth and development of maize. The factors may vary from severe warmth shortage in the North of the region to sharp aridity in the South. Consequently, fast-ripening maize breeding is dictated by limited heat resources. Depending on the growing area and the purpose of the crops (silage or corn), hybrids adapted for the regions must be characterised within the limits of 110 and 170 according to FAO classification. The purpose of the research is to measure ultra-early maize hybrids’ adaptability within forest-meadow and forest steppe zones of the Middle and the South Urals on maize’s growth stability and ripening, crop productivity and grain moisture at harvest. During the period from 1999 to 2019 experimental hybrid combinations and FAO 100-120 type industrial hybrids were assessed. These hybrids were created with the use of a local maize variety of Northern Caucasia, West Siberia and foothill areas of the Republic of Altay as a source material. Local maize varieties of Northern Caucasia, West Siberia and foothill areas of the Republic of Altay were used as initial material to create these hybrids. As a result of these three research phases early blossom maize varieties’ competitive advantages were discovered. These varieties’ grain formation and grain filling last within a favourable temperature background and have their biological ripeness before diurnal temperature achieves biological minimum required for maize growth. This advantage ensures minimum grain moisture at harvest and maximum genetic potentiality’s productivity implementation thanks to grain filling of full value. This advantage also assures high starch content and highly digestible energy concentration in dry matter. It has been established that ultra-early hybrids’ cultivation is the main condition to obtain high energy fodder in northern areas of the Urals despite of minor gross productivity loss. Commercial maize hybrids F1 Koubanskii 101 SV and Koubanskii 102 MV pass development stages from sprouts to ear corn blossom within the limits of 44 to 58 days depending on the vegetation period conditions. These hybrids’ cultivation in forest-steppe areas is of importance to produce dried and canned corn. Forest-meadow cultivation is of importance to obtain high grain canned products and high energy silage","PeriodicalId":53488,"journal":{"name":"International Journal of Biology and Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41968689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-09DOI: 10.46300/91011.2020.14.8
N. Bezborodova, O. V. Sokolova, I. Shkuratova, M. Ryaposova, Y. Lysova, M. Isakova, V. Kozhukhovskaya
This paper contains the results of complex microbiological studies (culture method, real-time PCR) of biological material from cows affected by inflammatory diseases of reproductive organs and mammary gland. Milk microbiota with underlying subclinical mastitis was represented by pathogenic (S. aureus, enteropathogenic E. coli), opportunistic bacteria (Staphylococcus spp., E. coli, E. faecium, Streptococcus spp., S. agalactiae, P. aeruginosa) and yeast-like fungi of Candida spp. in association. Combined infectious and inflammatory diseases of genital tract and mammary gland in cows resulted in the same types of microorganisms in microflora composition, which confirms information obtained by other researchers on associated microbiota during inflammatory processes. S. aureus, P. aeruginosa, E. coli, E. faecium, E. faecalis, S. epidermidis, S. saprophyticus, Bacillus spp., and yeast-like fungi of Candida spp. were obtained from milk samples from cows with acute clinical mastitis and in vaginal washes from cows with acute postpartum endometritis. In the course of defining the sensitivity to antibiotics and identifying genes of antimicrobial resistance, multidrug resistance was established in most part of obtained strains, which should be taken into account when planning treatment measures.
{"title":"Sensitivity and Resistance of the Microbiota of Reproductive Organs and Mammary Gland of Cows to Anti-microbial Agents in Cases of Inflammation","authors":"N. Bezborodova, O. V. Sokolova, I. Shkuratova, M. Ryaposova, Y. Lysova, M. Isakova, V. Kozhukhovskaya","doi":"10.46300/91011.2020.14.8","DOIUrl":"https://doi.org/10.46300/91011.2020.14.8","url":null,"abstract":"This paper contains the results of complex microbiological studies (culture method, real-time PCR) of biological material from cows affected by inflammatory diseases of reproductive organs and mammary gland. Milk microbiota with underlying subclinical mastitis was represented by pathogenic (S. aureus, enteropathogenic E. coli), opportunistic bacteria (Staphylococcus spp., E. coli, E. faecium, Streptococcus spp., S. agalactiae, P. aeruginosa) and yeast-like fungi of Candida spp. in association. Combined infectious and inflammatory diseases of genital tract and mammary gland in cows resulted in the same types of microorganisms in microflora composition, which confirms information obtained by other researchers on associated microbiota during inflammatory processes. S. aureus, P. aeruginosa, E. coli, E. faecium, E. faecalis, S. epidermidis, S. saprophyticus, Bacillus spp., and yeast-like fungi of Candida spp. were obtained from milk samples from cows with acute clinical mastitis and in vaginal washes from cows with acute postpartum endometritis. In the course of defining the sensitivity to antibiotics and identifying genes of antimicrobial resistance, multidrug resistance was established in most part of obtained strains, which should be taken into account when planning treatment measures.","PeriodicalId":53488,"journal":{"name":"International Journal of Biology and Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45240676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-25DOI: 10.46300/91011.2020.14.7
M. Tormozin, A. A. Zyryantseva
The article presents the results of studying the numbers of alfalfa variable in breeding nursery (2011- 2019 and 2015-2019) on the complex of economically valuable traits. High winter resistance of all varieties was revealed. On average, for three years in the breeding nursery the establishment of 2015-2019 years significantly exceeded the standard number on the seed yield: Victoria — by 70%, CHP-1 — 84%, CHP-2 — 24%, 192-92 (f) — 41%, 213 -11 — 74%. For three years of testing all these promising numbers significantly exceeded the Sarga variety (standard) in seeds yield. On average for three years the yield of green mass was 5.0-20.1 kg/10 m2 . According to this indicator the following samples significantly exceed the standard: Victoria — by 20.4%, 213-11 -5.1%, 199-06 — 15.0%. Dry matter collection was 1.08-4.27 kg/10 m2 (standard 3.78 kg/10 m2 ), excess of 4.5- 13.0%. During the longevity test in the breeding nursery of alfalfa (sowed in 2011) the following data was obtained: the seeds yield of breeding numbers in 2019 was 50.0 - 333.3 g from 10 m2 . The highest yield was noted in numbers: 20-89N (st), 203-06, 197-06/1, 27-86 (f), 199-06/1, 101-2 (st), 20-89N (st).
{"title":"Screening of Promising Selection Samples of Alfalfa Variable in Productivity and Longevity","authors":"M. Tormozin, A. A. Zyryantseva","doi":"10.46300/91011.2020.14.7","DOIUrl":"https://doi.org/10.46300/91011.2020.14.7","url":null,"abstract":"The article presents the results of studying the numbers of alfalfa variable in breeding nursery (2011- 2019 and 2015-2019) on the complex of economically valuable traits. High winter resistance of all varieties was revealed. On average, for three years in the breeding nursery the establishment of 2015-2019 years significantly exceeded the standard number on the seed yield: Victoria — by 70%, CHP-1 — 84%, CHP-2 — 24%, 192-92 (f) — 41%, 213 -11 — 74%. For three years of testing all these promising numbers significantly exceeded the Sarga variety (standard) in seeds yield. On average for three years the yield of green mass was 5.0-20.1 kg/10 m2 . According to this indicator the following samples significantly exceed the standard: Victoria — by 20.4%, 213-11 -5.1%, 199-06 — 15.0%. Dry matter collection was 1.08-4.27 kg/10 m2 (standard 3.78 kg/10 m2 ), excess of 4.5- 13.0%. During the longevity test in the breeding nursery of alfalfa (sowed in 2011) the following data was obtained: the seeds yield of breeding numbers in 2019 was 50.0 - 333.3 g from 10 m2 . The highest yield was noted in numbers: 20-89N (st), 203-06, 197-06/1, 27-86 (f), 199-06/1, 101-2 (st), 20-89N (st).","PeriodicalId":53488,"journal":{"name":"International Journal of Biology and Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45719791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-15DOI: 10.46300/91011.2020.14.6
B. Jeon
The analysis of the Ct and standard curve produced by real-time polymerase chain reaction (PCR) is a well-established method for the quantification of nucleic acids. However, this method assumes that the PCR efficiency between the unknown specimen and standard is equal, resulting in the possibility of significant inaccuracies due to the presence of inhibitory agents in the unknown specimen. Although numerous methods have been proposed to correct this issue, the understanding of the differences in PCR efficiencies in clinical samples is limited. In this study, 1185 cytomegalovirus (CMV) DNA real-time PCR test results from 106 batches were analyzed. The PCR efficiencies were calculated using the cpD2, maxE, Cy0, maxRatio and window-of-linearity (WoL) methods. The concentrations were calculated using the cpD2, Cy0, maxRatio, WoL, and take off point (TOP) methods. The coefficient of variation (CV) in the efficiency of the quantification standards was less than 5% in all methods. Positive samples with high quantification values demonstrated lower PCR efficiency compared to the quantification standards. This suggests possible inaccuracies in quantification using quantification standards in clinical samples.
{"title":"Variation in PCR Efficiencies between Quantification Standards and Clinical Specimens using Different Real-Time Quantitative PCR Interpretation Methods","authors":"B. Jeon","doi":"10.46300/91011.2020.14.6","DOIUrl":"https://doi.org/10.46300/91011.2020.14.6","url":null,"abstract":"The analysis of the Ct and standard curve produced by real-time polymerase chain reaction (PCR) is a well-established method for the quantification of nucleic acids. However, this method assumes that the PCR efficiency between the unknown specimen and standard is equal, resulting in the possibility of significant inaccuracies due to the presence of inhibitory agents in the unknown specimen. Although numerous methods have been proposed to correct this issue, the understanding of the differences in PCR efficiencies in clinical samples is limited. In this study, 1185 cytomegalovirus (CMV) DNA real-time PCR test results from 106 batches were analyzed. The PCR efficiencies were calculated using the cpD2, maxE, Cy0, maxRatio and window-of-linearity (WoL) methods. The concentrations were calculated using the cpD2, Cy0, maxRatio, WoL, and take off point (TOP) methods. The coefficient of variation (CV) in the efficiency of the quantification standards was less than 5% in all methods. Positive samples with high quantification values demonstrated lower PCR efficiency compared to the quantification standards. This suggests possible inaccuracies in quantification using quantification standards in clinical samples.","PeriodicalId":53488,"journal":{"name":"International Journal of Biology and Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41952266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-15DOI: 10.46300/91011.2020.14.5
J. Qar, M. A. Zoubi, Ibrahim Baydoun, A. Aljabali, Bahaa Al-Trad, Firas A Rabi, K. Batayneh
Hemorrhoids and varicose veins are conditions resulting from loss of vascular integrity and, despite being worldwide health concerns, their pathogenesis has not been clearly defined. Many risk factors have been linked to the development of these complications including diet, defecating habits, alcohol consumption and other physiological factors. There are limited studies involving the possible role of genetic mutations in the development of hemorrhoids and varicose veins. FoxC2 is an important transcription factor that plays many roles in a variety of embryonic developmental processes, including angiogenesis. In the current study, we aimed to investigate the role of the FOXC2 gene variations in the development of familial hemorrhoids and varicose veins in the Jordanian population. Thirty-two samples were collected from eight families manifested hemorrhoids and/or varicose veins conditions. DNA sequencing was performed to screen variation in the FOXC2 gene. Two individuals with severe and early onset of hemorrhoids and varicose veins from the same family showed a frameshift mutation (881'inT) in the coding exon of the FOXC2 gene resulting in a premature stop codon at position +1386 (294 residues truncated peptide). In conclusion, our results support a possible role of genetic predisposition in the development of hemorrhoids and varicose veins with a frequency of 6% in the selected population
{"title":"Truncating Mutation in FOXC2 Gene in Familial Hemorrhoids and Varicose Veins","authors":"J. Qar, M. A. Zoubi, Ibrahim Baydoun, A. Aljabali, Bahaa Al-Trad, Firas A Rabi, K. Batayneh","doi":"10.46300/91011.2020.14.5","DOIUrl":"https://doi.org/10.46300/91011.2020.14.5","url":null,"abstract":"Hemorrhoids and varicose veins are conditions resulting from loss of vascular integrity and, despite being worldwide health concerns, their pathogenesis has not been clearly defined. Many risk factors have been linked to the development of these complications including diet, defecating habits, alcohol consumption and other physiological factors. There are limited studies involving the possible role of genetic mutations in the development of hemorrhoids and varicose veins. FoxC2 is an important transcription factor that plays many roles in a variety of embryonic developmental processes, including angiogenesis. In the current study, we aimed to investigate the role of the FOXC2 gene variations in the development of familial hemorrhoids and varicose veins in the Jordanian population. Thirty-two samples were collected from eight families manifested hemorrhoids and/or varicose veins conditions. DNA sequencing was performed to screen variation in the FOXC2 gene. Two individuals with severe and early onset of hemorrhoids and varicose veins from the same family showed a frameshift mutation (881'inT) in the coding exon of the FOXC2 gene resulting in a premature stop codon at position +1386 (294 residues truncated peptide). In conclusion, our results support a possible role of genetic predisposition in the development of hemorrhoids and varicose veins with a frequency of 6% in the selected population","PeriodicalId":53488,"journal":{"name":"International Journal of Biology and Biomedical Engineering","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70491518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-05DOI: 10.46300/91011.2020.14.4
S. Kostarev, O. Kochetova, N. Tatarnikova, T. Sereda
Coronaviruses take the lead in gastrointestinal pathologies of animals and are spread all around the world. Causative agents of coronaviruses belong to Nidovirales order, Coronaviridae family which includes 2 subfamilies: Toroviridna (genera Torovirus and Bafinivirus) and Coronaviridna (genera Alphavirus, Betavirus, Gammavirus). Dividing of the latter by genera (groups I-III) was based on serological cross-reactions. Group I includes pathogens causing diseases in animals, such as swine coronavirus, feline infectious peritonitis virus. Group II includes pathogens of veterinary importance, such as BCoV, porcine hemagglutinating encephalomyelitis virus, horse coronavirus, viruses infecting mice and rats, as well as human coronaviruses that cause respiratory damage. Group III includes at the moment only avian coronaviruses. Coronavirus got its name in 1968 because of its special structure and appearance – it has spikes which resemble solar corona. In humans, coronavirus was first isolated by D. Tyrrell and M. Bynoe in 1965 from a patient with acute respiratory disease. Severe acute respiratory syndrome (SARS) in 2002, and then Middle East respiratory syndrome (MERS) in 2012 made specialists significantly increase the level of epidemic danger of coronaviruses. Due to its high virulence, virus multiplication rate in macrophages, pathogen replication, and antibody production increase significantly. At the end of 2019, coronavirus strain 2019-nCoV of Betacoronavirus genus was found in patients with pneumonia in China, and by early 2020 it spread all around the world. In animals, coronavirus leads to damage to mucous membranes. The amount of economic damage associated with disease caused by coronavirus is significant and includes death of animals, decreased meat and dairy productivity, decreased weight gain, culling, loss from abortion and infertility. In this regard, a demand arose for developing project of a device for express analysis for Coronaviridae antigen for the early diagnosis of coronaviruses. Based on the express analysis technique using solid-phase immuno-chromatographic medium, a project for express analysis for Coronaviridae antigen based on Omron industrial programmable logic controller was developed; it includes process chart for equipment, functional diagram and logical equations
{"title":"Project Design of a Device for Express Analysis for Coronaviridae Antigens based on Omron Industrial Programmable Logic Controller","authors":"S. Kostarev, O. Kochetova, N. Tatarnikova, T. Sereda","doi":"10.46300/91011.2020.14.4","DOIUrl":"https://doi.org/10.46300/91011.2020.14.4","url":null,"abstract":"Coronaviruses take the lead in gastrointestinal pathologies of animals and are spread all around the world. Causative agents of coronaviruses belong to Nidovirales order, Coronaviridae family which includes 2 subfamilies: Toroviridna (genera Torovirus and Bafinivirus) and Coronaviridna (genera Alphavirus, Betavirus, Gammavirus). Dividing of the latter by genera (groups I-III) was based on serological cross-reactions. Group I includes pathogens causing diseases in animals, such as swine coronavirus, feline infectious peritonitis virus. Group II includes pathogens of veterinary importance, such as BCoV, porcine hemagglutinating encephalomyelitis virus, horse coronavirus, viruses infecting mice and rats, as well as human coronaviruses that cause respiratory damage. Group III includes at the moment only avian coronaviruses. Coronavirus got its name in 1968 because of its special structure and appearance – it has spikes which resemble solar corona. In humans, coronavirus was first isolated by D. Tyrrell and M. Bynoe in 1965 from a patient with acute respiratory disease. Severe acute respiratory syndrome (SARS) in 2002, and then Middle East respiratory syndrome (MERS) in 2012 made specialists significantly increase the level of epidemic danger of coronaviruses. Due to its high virulence, virus multiplication rate in macrophages, pathogen replication, and antibody production increase significantly. At the end of 2019, coronavirus strain 2019-nCoV of Betacoronavirus genus was found in patients with pneumonia in China, and by early 2020 it spread all around the world. In animals, coronavirus leads to damage to mucous membranes. The amount of economic damage associated with disease caused by coronavirus is significant and includes death of animals, decreased meat and dairy productivity, decreased weight gain, culling, loss from abortion and infertility. In this regard, a demand arose for developing project of a device for express analysis for Coronaviridae antigen for the early diagnosis of coronaviruses. Based on the express analysis technique using solid-phase immuno-chromatographic medium, a project for express analysis for Coronaviridae antigen based on Omron industrial programmable logic controller was developed; it includes process chart for equipment, functional diagram and logical equations","PeriodicalId":53488,"journal":{"name":"International Journal of Biology and Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48439549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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