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Truncated Non-Nuclear Transposable Elements in Grapevine: A Mini Review 葡萄中截断的非核转座因子:综述
Q3 Agricultural and Biological Sciences Pub Date : 2019-12-01 DOI: 10.2478/sab-2019-0030
A. Milovanov, J. Tello, U. Anhalt, A. Forneck
Abstract In this mini-review we present insight to the non-nuclear transposable elements and in silico analysis of miniature inverted transposable elements (MITEs) in the grapevine mitochondrial genome. Here we report the identification of 17 truncated sequences in grapevine (Vitis vinifera L.) mitochondrial genome which expectedly belongs to the four ancient transposon families (hAT, Tc1Mariner, Mutator and PIF/Harbinger). Some sequences with a high rate of homology in chloroplast and nuclear genomes were also identified. Thus, it suggests the intercellular gene transfer between these three organelles. These partial sequences showed a high level of similitude with full MITE sequences, and they were found in their inner region, supporting their MITE origin. Further analysis revealed these sequences in other life kingdoms (including eubacteria and archaea), which indicates their ancient origin. Further research showed that 13 out of the 17 sequences are conserved domains of the genes where they are located, suggesting their contribution to gene evolution. Therefore, we suppose that more studies of nature, origin and functional meaning of these sequences and their fusion with genes are necessary. In the light of our observations it will be useful for further studies of V. vinifera genome organizing and systematics, as well as for other species.
摘要在这篇综述中,我们介绍了葡萄线粒体基因组中的非核转座元件和微型倒转座元件(MITEs)的硅分析。本文报道了葡萄(Vitis vinifera L.)线粒体基因组中17个截断序列的鉴定,预计它们属于4个古老转座子家族(hAT, Tc1Mariner, Mutator和PIF/Harbinger)。在叶绿体和细胞核基因组中也发现了一些同源率较高的序列。因此,这表明在这三个细胞器之间存在细胞间基因转移。这些部分序列与完整序列具有较高的相似性,且分布在其内部区域,支持其螨起源。进一步的分析显示,这些序列在其他生命王国(包括真细菌和古细菌)中也存在,这表明它们的起源很古老。进一步的研究表明,17个序列中有13个是它们所在基因的保守结构域,这表明它们对基因进化的贡献。因此,我们认为有必要对这些序列的性质、起源、功能意义及其与基因的融合进行更多的研究。根据我们的观察结果,将对进一步研究葡萄花的基因组组织和系统分类,以及其他物种有一定的参考价值。
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引用次数: 1
Canopy-Dwelling Arthropod Response to Rynaxypyr and Lambda-Cyhalothrin Treatments in Maize 玉米冠层节肢动物对赖氨酰和氯氟氰菊酯处理的反应
Q3 Agricultural and Biological Sciences Pub Date : 2019-12-01 DOI: 10.2478/sab-2019-0033
S. Keszthelyi, Z. Pónya
Abstract The technology of grain corn production has recently been continuously changing due to spreading of insecticidal in-crop treatments in Europe. The aim of these interventions is to prevent damage caused by serious lepidopterous pests in maize. We carried out in-maize field experiments using two different active ingredients of insecticides in four consecutive years (2014–2017). A field experiment was conducted to compare the effect of applications of rynaxypyr (ANT) and rynaxypyr + lambda-cyhalothrin (PYR) on the canopy-dwelling arthropod community in commercial maize grain acreage. The effects of both ANT and PYR treatments against Ostrinia nubilalis Hübner (Lep.: Crambidae) were tested through four-year field experiments. The quantitative and qualitative assemblages of the perished arthropods and diversity alterations measured by canopy netting and grounded tarpaulins greatly differed in the different insecticide treatments. A significant number of dead arthropods was recorded after PYR treatment. Populations of other natural enemies (Coccinellidae, Chrysopidae, etc.) and endangered species (Calomobius filum, Rossi) were also negatively affected. The arthropod community of the examined maize plots was drastically altered by sprayings, which, among other factors, may account for the mass appearance of the other non-target pest organisms (Aphidae: Rhopalosiphum spp., Miridae: Trygonotylus spp.).
摘要由于杀虫剂在欧洲作物处理中的推广,谷物玉米生产技术最近不断发生变化。这些干预措施的目的是防止玉米中严重的鳞翅目害虫造成的损害。我们连续四年(2014-2017年)使用两种不同的杀虫剂活性成分进行了玉米田间试验。通过田间试验,比较了在商品玉米种植区施用丙氧基PYR(ANT)和丙氧基PYR+高效氯氟氰菊酯(PYR)对冠层节肢动物群落的影响。通过为期四年的田间试验,测试了ANT和PYR处理对小虫Ostrinia nubilalis Hübner(Lep.:Crambidae)的效果。在不同的杀虫剂处理中,通过遮篷网和防水油布测量的死亡节肢动物的数量和质量组合以及多样性变化差异很大。PYR处理后记录到大量节肢动物死亡。其他天敌(球虫科、金鳞科等)和濒危物种(灰蝶、罗西)的种群也受到了负面影响。所检查的玉米地的节肢动物群落因喷洒而发生了巨大变化,除其他因素外,这可能是其他非目标害虫生物(蚜科:Rhopalosiphum spp.,Miridae:锥虫属spp.)大量出现的原因。
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引用次数: 0
Calcium Chloride and Drought Stress Changed Grain Yield and Physiological Traits in Sesame (Sesamum indicum L.) 氯化钙和干旱胁迫对芝麻籽粒产量和生理性状的影响
Q3 Agricultural and Biological Sciences Pub Date : 2019-12-01 DOI: 10.2478/sab-2019-0029
M. Heidari, N. Amirfazli, H. Ghorbani, F. Zafarian
Abstract Water deficit or drought stress is one of the most significant abiotic stresses that induce reduction in plant growth and crops yield. Calcium chloride has been shown to ameliorate the adverse effects of drought stress on many plants. Therefore, this study aimed to investigate the role of calcium chloride in drought resistance and its effect on some physiological characteristics in sesame. Calcium is essential for good growth and structure of plants. In sesame (Sesamum indicum L.), the foliar application of calcium chloride (C1=0 (control), C2=5, C3=10 and C4=15 mM concentration) significantly affected on grain yield under drought stress (W1= 7 (control, no drought stress-plants irrigation at a 7-day interval), W2= 12 (severe drought and plants irrigation at a 12- day interval) and W3= 17 (the most severe drought and plants irrigation at a 17-day interval). Drought stress reduced grain yield and 1000 seed weight, but these were enhanced by foliar application of calcium chloride when drought levels increased from W1 to W3. Although the increasing calcium chloride concentration reduced the content of photosynthesis pigments in leaves, the drought treatment until W2 increased the content of photosynthetic pigments (chlorophyll “a” and carotenoids) in leaves. The foliar application of calcium chloride increased the seed weight in plant, the number of capsules per plant, plant height, and the concentration of potassium and phosphorus in leaves and seeds. The greatest amount of potassium in leaves and seeds were measured under the W1C3 treatment. Sesame plants under W3C3 and W2C3 had the highest amount of phosphorus in leaves and seeds, respectively. Overall, although drought stress reduced the growth and grain yield in sesame, the foliar application of calcium chloride at the concentration of 10 mM, prevented the drought-stressed sesame plants from damage by improving their physiological parameters.
水分亏缺或干旱胁迫是引起植物生长和作物减产的最重要的非生物胁迫之一。氯化钙已被证明可以改善干旱胁迫对许多植物的不利影响。因此,本研究旨在探讨氯化钙在芝麻抗旱性中的作用及其对芝麻某些生理特性的影响。钙是植物良好生长和结构所必需的。在芝麻(Sesamum indicum L.)中,叶面施用氯化钙(C1=0(对照)、C2=5、C3=10和C4=15 mM浓度)对干旱胁迫(W1= 7(对照,无干旱胁迫,间隔7 d灌溉)、W2= 12(严重干旱,间隔12 d灌溉)和W3= 17(最严重干旱,间隔17 d灌溉)下的籽粒产量有显著影响。干旱胁迫降低了籽粒产量和千粒重,但当干旱水平从W1增加到W3时,叶面施用氯化钙提高了产量和千粒重。虽然增加的氯化钙浓度降低了叶片光合色素的含量,但直到W2的干旱处理增加了叶片光合色素(叶绿素“a”和类胡萝卜素)的含量。叶面施用氯化钙增加了植株籽粒重、单株蒴果数、株高以及叶片和种子中钾、磷的浓度。叶片和种子中钾含量以W1C3处理最高。W3C3和W2C3处理的芝麻植株叶片磷含量最高,种子磷含量最高。总体而言,尽管干旱胁迫降低了芝麻的生长和籽粒产量,但叶面施用浓度为10 mM的氯化钙通过改善芝麻植株的生理参数,防止了干旱胁迫对芝麻植株的伤害。
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引用次数: 2
Quality Attributes that Matters When Purchasing Milk by Slovak Consumers 斯洛伐克消费者购买牛奶时考虑的质量因素
Q3 Agricultural and Biological Sciences Pub Date : 2019-12-01 DOI: 10.2478/sab-2019-0034
K. Kurajdová, J. Táborecká-Petrovičová, G. Nedelová
Abstract Food market has changed dramatically over past decades. Whereas in the past consumers faced problems with food availability and quantity, today they are more concerned with its qualitative side. Milk, Slovak traditional foodstuff, is now experiencing the declining consumer interest. Regarding this, the main aim of the article is to examine the importance and influence of quality attributes on Slovak consumers when purchasing milk. For accomplishing this, a quantitative consumer survey was arranged. Its results showed that to the group of top five most important quality attributes belonged healthiness, freshness, sensory appeals, processing and fat; and a presence of at least two dependencies in relation to age, gender, income and education individually was confirmed. This creates a valuable basis for the value concept development, segmentation, targeting and positioning projection, product strategy modification and innovation and segmentation and marketing mix strategy creation.
摘要食品市场在过去的几十年里发生了巨大的变化。过去,消费者面临食品供应和数量方面的问题,而今天,他们更关心食品质量方面的问题。斯洛伐克传统食品牛奶的消费者兴趣正在下降。关于这一点,本文的主要目的是考察质量属性对斯洛伐克消费者购买牛奶的重要性和影响。为了做到这一点,安排了一项定量消费者调查。结果表明,前五名中最重要的品质属性分别为健康度、新鲜度、感官吸引力、加工性和脂肪;证实了在年龄、性别、收入和教育方面存在至少两种依赖关系。这为价值概念开发、细分、目标定位预测、产品战略修改和创新、细分和营销组合战略创建奠定了宝贵的基础。
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引用次数: 1
Identification of the Location of Vanished Objects of Baroque Land in Lysá Nad Labem 莱士<s:1>纳德拉贝姆巴洛克时期土地上消失物品的位置鉴定
Q3 Agricultural and Biological Sciences Pub Date : 2019-12-01 DOI: 10.2478/sab-2019-0032
M. Havlová
Abstract The case study focuses on localizing the vanished complex of Count Špork’s baroque landscape at Lysá nad Labem using aerial photography. The only source localizing the former historical objects is Vogt’s veduta from 1712. The objects were not captured in any historical cartographic materials, as they had disappeared before the first mapping of the territory. So no relevant information on the vanished objectsis at disposal. The results of the present study are rectification of the objects in the southwestern part of Vogt’s veduta and aerial images of the previously unspecified structures near the former hospital Karlov. The study provides more information on one of the largest vanished baroque complexes in Central Europe and opens up further for closer studies of this area including thebaroque landscape. At the same time, the effectiveness of aerial survey for the needs of landscape planning has been confirmed. Thanks to localizing the vanished monuments, they can be incorporated into the landscape planning system and approached as a cultural heritage, a value and a limit of the territory.
本案例研究的重点是使用航空摄影技术将Count Špork在lys nad Labem的巴洛克式景观的消失综合体本地化。对这些历史物品进行定位的唯一来源是沃格特1712年的吠陀经。这些物体没有被记录在任何历史制图材料中,因为它们在首次绘制该领土之前就消失了。所以没有关于失踪物品的相关信息。目前研究的结果是对Vogt veduta西南部的物体进行了校正,并对Karlov前医院附近以前未指明的结构进行了航拍。这项研究为中欧最大的消失的巴洛克建筑群之一提供了更多的信息,并为该地区包括巴洛克景观的更深入研究开辟了道路。同时,证实了航空测量对景观规划需求的有效性。由于将消失的古迹本地化,它们可以被纳入景观规划系统,并作为文化遗产、价值和领土的限制来对待。
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引用次数: 0
Future Agriculturists: Czech and U.S. Agricultural Students’ Attitudes Towards Agriculture 未来的农业学家:捷克和美国农业学生对农业的态度
Q3 Agricultural and Biological Sciences Pub Date : 2019-12-01 DOI: 10.2478/sab-2019-0035
H. Chýlová, P. Michálek, P. Rymešová, L. Natovová
Abstract The future of agriculture in the EU is endangered by a constantly diminishing number of young skilled agricultural workers starting their career in agribusiness. The problem of not pursuing in the agricultural career after the university graduation forced us to compare different attitudes of students from the USA (Ohio State University) and the Czech Republic (Czech University of Life Sciences Prague). The article deals with the attitudes towards agriculture in two groups of agriculture university students (n = 201). The data were collected with the use of a standardized Questionnaire on Measuring the Affective and Cognitive Properties of Attitudes. The results of Man-Whitney test indicate that both groups of students show significantly more the affective than the cognitive base of their attitude. Furthermore, U.S. students display more positive attitudes towards the agriculture workers than Czech students. Authors bring forward the issue of attitudes in an attempt to find a way to influence the decision making of skilled agri-graduates to prefer the field of agribusiness over other careers and propose to focus on a change in attitudes, for example by the increase of the level of practical experience with agribusiness in school farm estates.
摘要欧盟农业的未来正受到越来越少的年轻技术农业工人在农业综合企业开始职业生涯的威胁。大学毕业后不从事农业职业的问题迫使我们比较美国(俄亥俄州立大学)和捷克共和国(捷克布拉格生命科学大学)学生的不同态度。本文研究了两组农业大学生(n=201)对农业的态度。这些数据是使用标准化的测量态度的情感和认知特性问卷收集的。Man-Whitney测试的结果表明,这两组学生在态度上都表现出了更多的情感基础,而不是认知基础。此外,美国学生对农业工人表现出比捷克学生更积极的态度。作者提出了态度问题,试图找到一种方法来影响技术农业毕业生的决策,使他们更喜欢农业综合企业领域而不是其他职业,并建议重点改变态度,例如提高学校农场农业综合企业的实践经验水平。
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引用次数: 0
Influence of Canahua and Quinoa Wholemeals on Properties of Non-Fermented Wheat Dough Canahua和Quinoa全麦对未发酵小麦面团性质的影响
Q3 Agricultural and Biological Sciences Pub Date : 2019-12-01 DOI: 10.2478/sab-2019-0031
I. Švec, M. Hrušková, R. Kapačinskaitė, T. Hofmanová
Abstract The study compares the influence of wheat flour replacement by 10 or 20 wt% of quinoa and canahua wholemeals on wheat dough technological quality and rheological properties. The technological quality of wheat flour was affected in terms of protein quality and amylases activity, associated with a high dietary fibre content of both tested non-traditional materials. A farinograph test revealed that quinoa partially increased water absorption; a higher amount of water resulted in the shortened stability of dough consistency during mixing and its weakened cohesiveness at the end of the test. The effect of canahua was unequivocal – water absorption decreased, stability was prolonged properly, but dough softening increased. An extensigraph test confirmed a positive effect of alternative crops on dough elasticity, but in general, the composite dough with 20% of canahua or quinoa showed worsened machinability. Multivariate statistical methods proved a stronger effect when quinoa was analysed solely than when added at complete samples a set, while for canahua-wheat samples the result was opposite.
本文比较了用10%或20%的藜麦和加拿大全麦代替小麦粉对小麦面团工艺质量和流变性能的影响。小麦粉的工艺质量受到蛋白质质量和淀粉酶活性的影响,这与两种测试的非传统材料的高膳食纤维含量有关。粉质测试显示藜麦部分增加了吸水率;较高的水量导致面团在混合过程中稠度的稳定性缩短,并且在测试结束时其内聚性减弱。卡纳华的效果是明确的——吸水率降低,稳定性适当延长,但面团软化度增加。一项延伸图测试证实了替代作物对面团弹性的积极影响,但总的来说,含有20%卡纳瓦或藜麦的复合面团显示出较差的可加工性。多元统计方法证明,单独分析藜麦比在一组完整样本中添加藜麦效果更强,而对于加拿大小麦样本,结果则相反。
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引用次数: 0
Isolation, Screening and Response of Maize to Plant Growth Promoting Rhizobacteria Inoculants 玉米对植物生长促进根际细菌菌种的分离、筛选及响应
Q3 Agricultural and Biological Sciences Pub Date : 2019-09-01 DOI: 10.2478/sab-2019-0025
A. Akintokun, E. Ezaka, P. Akintokun, O. Shittu, L. Taiwo
Abstract The use of plant growth promoting rhizobacteria (PGPR) as biofertilizer is a relatively safer, environment friendly and cost effective. This work was designed to assess plant growth promoting abilities of rhizobacteria and evaluate their effect on germination and growth of maize. The bacteria were isolated and screened for plant growth promoting abilities using Pikovskaya agar, Aleksandrov agar and Jensen media. Twelve isolates that showed multiple attributes were further screened for indole acetic acid (IAA) and gibberellic acid (GA) production; best five isolates were selected for further studies. The results of IAA and GA production showed a considerable amount of IAA and GA produced by the isolates which ranged between 9–94 and 21–97 mg l–1, respectively. The selected isolates identified as Bacillusmojavensis, Pseudomonas aeruginosa, Alcaligenes faecalis, Pseudomonas syringae and Bacillus cereus showed a significant difference(P≤0.05) in the amount of potassium(K) and phosphorus(P) solubilized at different source of K (KCl and mica powder) and P (Ca2PO4 and rock phosphate). The isolates also recorded significant level of nitrogen fixing ability with Alcaligenes faecalis strain P156 fixing the highest amount of nitrogen (11.4 mg N fixed per l) and least by Bacillus mojavensis strain NBSL51 (6.3 mg N fixed per l).The results of plant inoculation test showed that Bacillus cereus strain 20UPMNR significantly enhanced the root and shoot dry weight. All the selected isolates enhanced shoot and root length except Bacillus mojavensis which produced less effect on root length when compared to the control. These results have provided vital information for the development of a bio fertilizer for maize.
摘要利用植物促生根瘤菌(PGPR)作为生物肥料是一种相对安全、环保、经济的方法。本研究旨在评价根细菌对玉米种子萌发和生长的促进作用。分别用Pikovskaya琼脂、Aleksandrov琼脂和Jensen培养基对细菌进行了分离和促生长筛选。对12株具有多种性状的菌株进行进一步筛选,以产吲哚乙酸(IAA)和赤霉素酸(GA);筛选出5株最佳菌株进行进一步研究。IAA和GA的产量结果表明,菌株IAA和GA的产量分别在9-94和21-97 mg l-1之间。分离菌株mojavensis、Pseudomonas aeruginosa、Alcaligenes faecalis、Pseudomonas syringae和Bacillus cereus在不同K (KCl和云母粉)和P (Ca2PO4和岩磷矿)来源下的钾(K)和磷(P)溶出量差异显著(P≤0.05)。菌株P156的固氮量最高(11.4 mg / l),而菌株NBSL51的固氮量最低(6.3 mg / l)。植株接种试验结果表明,蜡样芽孢杆菌20UPMNR显著提高了根和茎干重。除莫氏芽孢杆菌对根长影响较小外,其余菌株均能显著提高植株的茎长和根长。这些结果为玉米生物肥料的开发提供了重要信息。
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引用次数: 15
Detection of Animal Occurrence Using an Unmanned System 使用无人系统检测动物事件
Q3 Agricultural and Biological Sciences Pub Date : 2019-09-01 DOI: 10.2478/sab-2019-0028
J. Lešetický, P. Matějka, M. Olmr
Abstract In recent decades, there has been an increase in the work speed and breadth of agricultural technology used to mow grasses. This modernization has resulted in a decline in wildlife. There are several conventional ways to prevent these losses. The most well-known and simplest technique is to search for wild animals using dogs and a phalanx. The dogs are trained to systematically search the area and drive the animals out. Efficiency is increased when visiting a site regularly, thus disturbing the animals, which are then consequently less likely to fawn. The effectiveness of the swarm line depends on the number of participants involved. The recommended spacing is set at 1–3 m. An effective modern means seems to be the use of an unmanned system and thermal cameras. This article presents a proof of concept of a detection system that is capable of detecting the object searched for in grassy vegetation with more than 96% success, regardless of the flight level. The study contributes to automated detection based on the basic principles of threshold.
摘要近几十年来,用于割草的农业技术的工作速度和广度都在提高。这种现代化导致了野生动物数量的减少。有几种传统的方法可以防止这些损失。最著名和最简单的技术是用狗和指骨寻找野生动物。训练这些狗系统地搜索该地区并将动物赶走。当定期访问一个地点时,效率会提高,从而干扰动物,从而减少它们的小鹿。群线的有效性取决于参与者的数量。建议的间距设置为1-3米。一种有效的现代手段似乎是使用无人系统和热像仪。本文提出了一种检测系统的概念验证,该系统能够检测在草地植被中搜索的物体,无论飞行高度如何,成功率都超过96%。这项研究有助于基于阈值基本原理的自动检测。
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引用次数: 0
Muscle Fibre Types and Their Relation to Meat Quality Traits in Pigs 猪肌纤维类型及其与肉质性状的关系
Q3 Agricultural and Biological Sciences Pub Date : 2019-09-01 DOI: 10.2478/sab-2019-0022
N. Lebedová, R. Stupka, J. Čítek, K. Zadinová, E. Kudrnáčová, M. Okrouhlá, P. Dundáčková
Abstract The authors have been studying various characteristics of muscle fibres and their relationship to the meat quality parameters for many years. However, the conclusions drawn by researchers often differ. A higher proportion of glycolytic IIB fibres in pig muscles is usually related to paler meat with lower water holding capacity. On the other hand the relationship between muscle fibres and meat texture parameters is not clear. Studies using immunohistochemistry methods that allow a more detailed classification of individual muscle fibre types could bring new findings in this area. It would thus be possible to influence muscle fibre type composition in the muscle to achieve the desired meat quality using various extrinsic and intrinsic factors. The main aim of this review is to summarise current knowledge on the description of muscle fibres typology and the effect of their morphological traits on pork meat quality.
摘要作者多年来一直在研究肌肉纤维的各种特性及其与肉质参数的关系。然而,研究人员得出的结论往往不同。猪肌肉中糖酵解IIB纤维比例较高通常与含水量较低的浅色肉有关。另一方面,肌肉纤维与肉质参数之间的关系尚不清楚。使用免疫组织化学方法对单个肌肉纤维类型进行更详细的分类的研究可能会在这一领域带来新的发现。因此,可以利用各种外在和内在因素来影响肌肉中的肌肉纤维类型组成,以达到所需的肉质。这篇综述的主要目的是总结目前关于肌肉纤维类型描述及其形态特征对猪肉品质的影响的知识。
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引用次数: 1
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Scientia Agriculturae Bohemica
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