Annals of Agricultural Science最新文献

Sperm motility, the percentage of sperm with forward progressions, is one of the semen quality traits that is highly related with male fertility potential in farm animals. The proportion of roosters with low sperm motility impedes the reproduction efficiency. This study aimed to determine crucial testicular proteins linked to sperm motility defects as causes of subfertility in roosters. iTRAQ was performed with the testis from five low sperm motility roosters and three high ones. A total of 2345 proteins were identified, of which, 86 were differentially expressed (DEPs), including 5 up-regulated and 88 down-regulated proteins in the low group. These DEPs were mainly enriched in cytoskeleton and cilium cell component, and biological processes related to sperm motility, and protein transport, and cellular protein localization. A cluster of 13 down-regulated proteins such as IFT88, TEKT1, ACTN2, DNAH5, RSPH9, and SPAG6, were associated with axoneme assembly. Further western blot and immunohistochemical analysis confirmed the down-regulated SPAG6 expression in low group, and indicated its expression in other cell types in testis beyond round spermatids, and that its pattern was in accordance with testis development and recession pace. Silencing transcription factor SOX5 down-regulated SPAG6 transcripts and impaired the cell proliferation and migration. In summary, this study highlights that down-regulated sperm flagellar structure associated proteins were the potential cause of low sperm motility. More specifically, the highly conserved SPAG6 protein across species is a positive regulator for testis development, spermatogenesis, and sperm motility regulation in chickens.

Large quantities of waste generated from papaya fruit processing, especially seeds, can be recovered and combined to obtain added-value products. This investigation aimed to evaluate the physicochemical properties and antimicrobial activity of papaya seed powder (PSP) and papaya seed oil (PSO), and their applications as novel nontraditional food ingredients in cupcakes to promote them as high-value products. The GC–MS analysis for PSP revealed 39 compounds possess various biological activities, indicating the safety of its consumption. Papaya seeds are an excellent source of protein (27.95 %), oil (31.83 %), ash (7.86 %) and fibers (18.53 %). They have the potential to produce high-quality oleic oil with highly similar physicochemical properties and fatty acids profile as olive oil. The broad-spectrum inhibition of PSP and PSO against the tested pathogens proved their potential as natural antimicrobial agents. PSP can successfully be used (as nutritive ingredients and antimicrobial agents) in combination with wheat flour to obtain delicious and healthy nutritious chocolate cupcakes. The supplemented cupcakes (up to 15 % level) had improved quality attributes and enhanced nutritional value with respect to protein (16.89 %), ash (4.17 %) and fibers (3.28 %). PSP and PSO are safety, edible, nutritious, antimicrobial and can be processed into value-added products. They can be used as novel functional food ingredients or natural additives, providing added value to the food processing.

A 2-year field experiment was conducted to analyse the effects of fresh and aged biochar on soil properties, as well as herbicide effectiveness and rice-yield components in a Mediterranean environment. Six managements were used: no-tillage and sprinkler irrigation either without (NoT) or with first-year biochar application (NoTB), conventional tillage and sprinkler irrigation either without (ConvT) or with first-year biochar application (ConvTB), conventional tillage and flooding irrigation either without (ConvTF) or with first-year biochar application (ConvTFB). The measurements done in the first (2018) and second (2019) years after biochar addition were taken to determine its fresh and aged effects, respectively. The application of biochar led to an improvement of the soil properties such as increase in the soil's organic carbon content, pH, and dehydrogenase activity. It also reduced weed pressure, regardless of the management used, and sprinkler systems were found to have the greatest herbicide effectiveness, especially in ConvT and ConvTB. In terms of production, there were significant increases in yield with the application of fresh biochar, in the amended managements under tillage compared with their unamended counterparts. The greatest yields were found under ConvT and ConvTB, with mean values for both treatments of 10,770 and 11,299 kg ha⁻¹ in the fresh and aged years, respectively. Furthermore, water productivity reached its greatest values when sprinkler was used, especially with tillage for which the application of fresh biochar increased water productivity by a factor of 1.09 relative to ConvT. Therefore, sprinkler irrigation in combination with tillage and biochar applications can be regarded as an efficient alternative to flooding irrigation in order to ensure productive viability of rice in water-stressed regions.

Iron (Fe) plays a pivotal role in biogeochemical cycling in the soil-plant-human continuum; however, much research has focused on Fe deficiency with limited knowledge about Fe toxicity impacts on micronutrient (e.g., zinc: Zn) accumulation in cassava, an important staple tuber food in the Tropics. A sand-hydroponic-hybrid technique was undertaken for 60 days to investigate the effects of variable Fe concentrations (0.1–500 μmol Fe L⁻¹) on the root-proton and root-organic acid release, rhizosphere Fe solubility, and levels of cassava nutrient accumulation. The results showed that high Fe concentrations (50–500 μmol Fe L⁻¹) significantly decreased the root (0.34–0.51 g dry weight) biomass compared to the control (0.82–0.83 g dry weight). The Fe concentrations significantly regulated the net proton release from the roots by upregulating (25–45 μmol L⁻¹ h⁻¹ g⁻¹ fresh weight root) and downregulating (1.8–7.1 μmol L⁻¹ h⁻¹ g⁻¹ fresh weight root) the net proton efflux at low (1.0 μmol Fe L⁻¹) and high Fe concentrations (500 μmol Fe L⁻¹), respectively. Succinic acid was the sole organic acid observed and only in the Fe-sensitive cultivar. The water-extractable Fe in the rhizosphere sand (average 3.37 mg kg⁻¹) was lower than in the bulk sand (average 5.31 mg kg⁻¹), indicating that net proton efflux controlled rhizosphere Fe solubility. The high Fe concentrations significantly decreased Zn accumulation (20–29 mg Zn kg⁻¹) but increased phosphorus (P) accumulation (2.7–3.4 g P kg⁻¹) in the roots compared to the control (48–59 mg Zn kg⁻¹ and 2.0–2.3 g P kg⁻¹, respectively), implying that Fe toxicity could be responsible for the undernourished Zn content in the tuber. This study suggested the importance of alleviating Fe toxicity in soil-plant systems to mitigate cassava root Zn deficiency, which is relevant to human Zn malnutrition in countries consuming cassava as the main staple diet.

Study aimed to investigate the impact of long-term high ambient temperature (HAT) exposure and dietary antioxidant supplementation (elevated levels and in combination) on nutrient digestibility, metabolism and immune response of pigs. A total of 36 Danbred barrows (65.1 ± 2.81 kg) were allotted to four treatments: Trm1: HAT (28.9 ± 0.9 °C) + control diet (CD); Trm2: HAT + elevated vitamin C and E and Se and Zn; Trm3: HAT + further elevated vitamin C and E and Se and Zn; and Trm4: thermo-neutral ambient temperature (19.5 ± 0.9 °C) + CD. Nutrients (DM, CP, Cfat, CF, CA, GE) and minerals (Ca, P, Na, Zn, Se) digestibility were determined. Plasma metabolite and cytokine concentrations were investigated from the blood samples collected on d 15 and 28. Heat shock proteins (HSP 70 and 90) and tumor necrosis factor-alpha (TNF-α) expressions were investigated from jejunum samples of pigs. HAT did not significantly affect pigs' nutrient digestibility and retention (P > 0.05). However, Trm2 pigs had greater digestibility in terms of DM, CF, Ca, Zn, Se and retention of Ca, Zn, Na, and Se than Trm1 and Trm4 groups. Trm3 pigs had significantly higher (P < 0.05) creatinine concentrations than Trm4 pigs. HAT did not significantly affect the expression of cytokines; however, vitamins and micro-minerals supplementation in the diet significantly (P < 0.05) improved interleukin (IL) 10 expression, reduced TNF-α, and HSP70 expressions. Vitamin and micro-mineral fortified diet can improve pigs' nutrient and mineral digestibility and could alleviate inflammatory response in pigs exposed to HAT.

Jerusalem artichoke is known as a good source of inulin beside other essential bioactive molecules that may promote human health. In the present study the Jerusalem artichoke powder (JAP) was used for fortification of dumplings' dough in different incorporation levels (2, 2.5, 5, 7.5 and 10 %). Furthermore, the physicochemical, rheological, and organoleptic characteristics have been evaluated for the fortified dumplings and developing a novel and functional an inulin-rich dumplings' dough. Jerusalem artichoke showed a fiber content (21.4 ± 0.26 %) with high content of inulin and pectin (38.70 ± 0.19 % and 13.5 ± 0.36 % out of total carbohydrates respectively). JAP containing calcium, potassium and iron (3.9, 2.75 and 7.48 times respectively) more than wheat flour. Thiamin, riboflavin and niacin showed a content 4.47, 84.5 and 5.7 times higher than wheat flour. JAP is containing an ascorbic acid (23.57 ± 2.15 mg/100) and β-carotene (57.14 ± 1.45 mg/100 g) which are not existing in wheat flour. Adding up to 7.5 % of Jerusalem artichoke powder improved the rheological and organoleptic properties of dough. The fortified dumplings' dough prepared with 7.5 % of JAP recorded the highest sensory score (∼8 out of 9) and preferred by the panelists. In conclusion the fortification of dumpling's dough with up to 7.5 % JAP has improved the nutritional, rheological and organoleptic properties of the product which is promising for a wider application in different functional bakery products.

Globally, around 800 million hectares are affected by salinity. This abiotic stress causes plant growth inhibition, disruptions in physiological processes in plant cells, and yield losses in many crops. Sunflower is the third-most oilseed crop globally produced, and it is considered moderately tolerant to salinity. There are few studies about the genotypic variability existing in sunflower for responses to salinity, especially the changes in yield and oil content and quality under salinity. The present work aimed to study the effects of salinity on achene yield in four sunflower genotypes and their components and on the oil content and quality and their relationships. Four sunflower hybrids (ACA885, TRITON MAX, SRM769 and SRM779) were grown at 130 mM NaCl irrigation solution under controlled environmental conditions. The achene yield, the yield components, and the content and oil quality were determined. Based on the four studied genotypes, salinity decreased achene yield by 75.1 %. SRM779CL had a lower achene yield reduction by salinity. Yield component that most explained this tolerance was the number of achenes per plant. SRM779CL was the hybrid with the highest oil percentage loss. Contrarily, salinity increased the oil content in ACA885. Salinity decreased the ratio between oleic (18:1) and linoleic acid (18:2) in all genotypes. Therefore, salt stress increased the percentage of unsaturations in the four genotypes examined in this work. Finally, considering the 12 parameters measured, principal components analysis could determine that SRM779CL showed the best performance under control conditions while ACA885 was the most tolerant under salinity.